Batimastat (BB-94)

Catalog No.S7155 Batch:S715502

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Technical Data

Formula

C23H31N3O4S2
Molecular Weight 477.64 CAS No. 130370-60-4
Solubility (25°C)* In vitro DMSO 96 mg/mL (200.98 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 6.000mg/ml (12.56mM) Taking the 1 mL working solution as an example, add 50 μL of 120 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.
Targets
MMP-1 [1] MMP-2 [1] MMP-9 [1] MMP-7 [1] MMP-3 [1]
3 nM 4 nM 4 nM 6 nM 20 nM
In vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. [1]

Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. [2]
In vivo

Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. [1]

In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).[3]

Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. [4]

Protocol (from reference)

Kinase Assay:

[1]
  • Enzyme assays

    IC50s are determined for batimastat in vitro against different metalloproteinases in enzyme assays.
Cell Assay:

[1]
  • Cell lines

    B16-BL6 cells

  • Concentrations

    3 nM (MMP-1), 4 nM (MMP-2 & MMP-9), 6 nM (MMP-7) and 20 nM (MMP-3), respectively

  • Incubation Time

    22 h

  • Method

    Cells were treated with different concentrations of batimastat was dissolved in absolute ethanol and were incubated for 22 hr and IC50 was measured.
Animal Study:

[3]
  • Animal Models

    Nude mouse

  • Dosages

    30mg/ml

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/8050828/
  • https://pubmed.ncbi.nlm.nih.gov/8610113/
  • https://pubmed.ncbi.nlm.nih.gov/8062271/
  • https://pubmed.ncbi.nlm.nih.gov/7535861/

Customer Product Validation

Proliferation indices of SU-pcGBM2 cells exposed to plain medium (aCSF) or active conditioned medium generated in the presence or absence of pan-MMP inhibitor (BAT).

Data from [ , , Nature, 2017, 549(7673):533-537 ]

To assess cleavage, 2 μg of pro-TNF-α was incubated with either 2 μg ADAM17 or 2 μg MMP9 in the presence or absence of inhibitor. Lanes are as follows: 1. Molecular weight markers, 2. Pro-TNF-α alone, 3. Pro-TNF-α + ADAM17, 4. pro-TNF-α+ ADAM17 + BB-94 (10 μM), 5. Pro-TNF-α + MMP9, 6. Pro-TNF-α + MMP9 + AB0041 (10 μM), 7. Pro-TNF-α+ MMP9 + BB-94 (10 μM), 8. Soluble TNF-α.

Data from [ , , PLoS One, 2015, 10(5): e0127063 ]

Effect of the broad spectrum hydroxamate inhibitors batimastat and ilomastat on a disintegrin and metalloproteinase with thombospondin type 1 motifs (ADAMTS) 13 and ADAMTS15. (A) While batimastat is a good inhibitor against ADAMTS15, ilomastat has no ability to inhibit the enzyme. ADAMTS15 (20 nM) was pre-incubated with two doses (300 nM or 3 µM) of batimastat and ilomastat prior to the addition of aggrecan substrate G1-IGD-G2. The inhibitory effect of batimastat can be clearly discerned from the undigested 130 kDa G1-IGD-G2 bands. (B) Calculation of the Kiapp value of batimastat by densitometric analysis of the undigested G1-IGD-G2 bands on a 12% SDS-PAGE.

Data from [ , , Biomed Rep, 2016, 4(1):73-78. ]

Calculation of the Ki<sup>app</sup> value of batimastat by densitometric analysis of the undigested G1-IGD-G2 bands on a 12% SDS-PAGE.

Data from [ , , Biomed Rep, 2016, 4(1):73-78 ]

Selleck's Batimastat (BB-94) Has Been Cited by 39 Publications

A pancreatic cancer organoid biobank links multi-omics signatures to therapeutic response and clinical evaluation of statin combination therapy [ Cell Stem Cell, 2025, S1934-5909(25)00265-6] PubMed: 40812300
The late-onset Alzheimer's disease risk factor RHBDF2 is a modifier of microglial TREM2 proteolysis [ Life Sci Alliance, 2025, 8(5)e202403080] PubMed: 40081988
UNC5B is an isoform-dependent target for ectodomain shedding [ J Biochem, 2025, mvaf043] PubMed: 40631620
Functional Differences Between SIRPα Splice Isoforms [ Genes Cells, 2025, 30(5):e70041] PubMed: 40763927
The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress [ bioRxiv, 2025, 2025.07.13.663563] PubMed: 40791532
Omicron Spike confers enhanced infectivity and interferon resistance to SARS-CoV-2 in human nasal tissue [ Nat Commun, 2024, 15(1):889] PubMed: 38291024
Amino-terminally elongated Aβ peptides are generated by the secreted metalloprotease ADAMTS4 and deposit in a subset of Alzheimer's disease brains [ Neuropathol Appl Neurobiol, 2024, 50(3):e12991] PubMed: 38867123
Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions [ Adv Sci (Weinh), 2023, e2207084.] PubMed: 36737855
FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid rafts [ Cancer Gene Ther, 2023, 30(4):608-621] PubMed: 36494579
Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation [ Sci Adv, 2023, 9(7):eade8939] PubMed: 36791204

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.