Choose Your Country or Region

Panobinostat (LBH589)

Name: Panobinostat (LBH589)
Brand: Selleck Chemicals
SKU: S1030
Rating: 5 (320 reviews)

Catalog No.S1030 Synonyms: NVP-LBH589

For research use only.

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.

Panobinostat (LBH589) Chemical Structure

CAS No. 404950-80-7

Selleck's Panobinostat (LBH589) has been cited by 320 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Related Compound Libraries

Other HDAC Products

Download Inhibitor Catalog

HDAC Signaling Pathway Map

Biological Activity

Description

Targets

HDAC (MOLT-4 cells) ^[1]	HDAC (Reh cells) ^[1]
5 nM	20 nM

In vitro

LBH589 induces apoptosis among MOLT-4 and Reh cells in a time- and dose-dependent manner. Moreover, LBH589 is more potent in MOLT-4 than in Reh cells. LBH589 markedly prevents the growth of both MOLT-4 and Reh cells in a dose-dependent manner at 48 hours. LBH589 treatment causes a 2- to 3-fold increase in the number of cells in the G2/M phase of the cell cycle compared with the control cells. LBH589 is associated with induction of histone H3K9 and histone H4K8 acetylation as well as decreasing levels of c-Myc expression in a dose-dependent manner. LBH589 treatment also increases the levels of p21 expression. LBH589 treatment also decreases the levels of c-Myc after an initial increase at the lowest dose (10 nM) in Reh cells. In addition, LBH589 gives rise to substantial increases in mRNA levels of proapoptosis and DNA repair genes. LBH589 induces increased levels of acetylated histone H3 and H4 at the GADD45G promoter. ^[1] Besides, LBH589 inhibits growth of non small cell lung cancer cell lines (such as human H1299, L55 and A549 with IC50 of 5 nM, 11 nM and 30 nM, respectively), mesothelioma (such as human OK-6 and Ok-5 with IC50 of 5 nM and 7 nM, respectively) and small cell lung cancer cell lines (such as human RG-1 and LD-T with IC50 of 4 nM and 5 nM, respectively). ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HT29	NWfmXG5xT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MUKwMVExKM7:TR?=	NHvRVnIxNTRiZB?=		MYfpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ?	NH;IPXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewNlc5PCd-Mk[3NFI4QDR:L3G+
HepG2	NHy3[IJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHHlVIwxNTFyIN88US=>	MVywMVQh\A>?		NV\LdllxcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	MmPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO\|g1RC:jPh?=
HT29	NYPk[ZpbTnWwY4Tpc44hSXO\|YYm=	M2fKVVUxyqCwTR?=	M1HL[FI1NTd{IHi=		Mm\mbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UhOyCjZoTldkA1QMLiaNMg	MnX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO\|g1RC:jPh?=
HepG2	NILIVJJHfW6ldHnvckBCe3OjeR?=	MWW1NOKhdk1?	MWKyOE04OiCq		MUPpcoR2[2WmIHHjeIl3[XSrb36gc4Yh[2G\|cHHz[UA{KGGodHXyJFI1yqCqwrC=	M4XGNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{CyO\|g1Lz5{NkewNlc5PDxxYU6=
HCC827	M3\6RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2S0VFUwPy53L{GwJI5O	M2PjZVczyqCq	MW\EUXNQ	NX3RZmFE\W6qYX7j[ZMhfGinIHHueIlxem:uaX\ldoF1cX[nIHXm[oVkfCCxZjDldoxwfGmwaXK=	NUnMOFNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2O\|U1QDRpPkK2Olc2PDh2PD;hQi=>
A549	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUfsVJA2OTBxMUWvNlAhdk1?	NV;2SpRsPzMEoHi=	M4rLd2ROW09?	M2[5N4VvcGGwY3XzJJRp\SCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5Qhd2ZiZYLsc5Rqdmmk	NGfrTVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[3OVQ5PCd-Mk[2O\|U1QDR:L3G+
NCI-H460	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mn:2NVAwOjBxM{Cgcm0>	NHrZW4s4OsLiaB?=	NVvpcpJ4TE2VTx?=	M2jiPYVvcGGwY3XzJJRp\SCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5Qhd2ZiZYLsc5Rqdmmk	M2iwfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nke1OFg1Lz5{Nk[3OVQ5PDxxYU6=
J89GFP	M2HvTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MnqzSG1UV8Li	M{XwVmVEPTB;NEmuPFUhyrFiMUKuOlUhdk1?	NVPDN3F[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlM2PjhpPkK2OVY{PTZ6PD;hQi=>
THP89GFP	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NVH2cmtiTE2VT9Mg	NEHzRZZGSzVyPUG5MlM1KMLzIE[uOFMhdk1?	NEPxbmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2N\|U3QCd-Mk[1OlM2Pjh:L3G+
SK-NEP-1	MnTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUTNUVB7OC5yMfMAl\|ExNjBizszN	NE\zRokzPCCq	M2jyTGROW00EoB?=	NH\TVJJKSzVyPUe2MlM1KG6P	M2fmU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
G401	NHqyO2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3fnd\|AvODIkgKOxNE4xKM7:TR?=	NWnBSXI{OjRiaB?=	M4DQU2ROW00EoB?=	M365NWlEPTB;MUSzMlAzKG6P	MlHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
SK-NEP-1	NH33dYhE\WyuIG\pZYJqdGm2eTDBd5NigQ>?	M4HyclUxKG6P	MkG1NgKBmzRiZB?=	NELjSJFFVVORwrC=	M1XReJJm\HWlZYOgZ4VtdCC\|dYL2bZZidCCrbjDhJJRqdWViZHXw[Y5l\W62IH3hco5meg>?	M3\vO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
G401	MWTD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	NUHCe\|F2PTBibl2=	M2nOXlHjiJN2IHS=	NFTyTZZFVVORwrC=	MnTndoVlfWOnczDj[YxtKHO3co\peoFtKGmwIHGgeIlu\SCmZYDlcoRmdnRibXHucoVz	M1W3N\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
SK-NEP-1	NGfUW3hCeG:ydH;zbZMhSXO\|YYm=	M{DGZ\|UxNzFyMDDuUS=>	NUPUe\|EyOjRiaB?=	NFG1fIJFVVORwrC=	MWfpcoR2[2W\|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401	MXrBdI9xfG:\|aYOgRZN{[Xl?	MlvMOVAwOTByIH7N	MWKyOEBp	NWTTfW4{TE2VT9Mg	NXLvV5BJcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	MmjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
SK-NEP-1	NWPKblh{TnWwY4Tpc44hSXO\|YYm=	MmDPOVAwOTByIH7N	MmXmNlQhcA>?	MnLDSG1UV8Li	M4TiVZNpd3e\|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25?	NVfjdHRoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO\|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401	MVjGeY5kfGmxbjDBd5NigQ>?	MnvSOVAwOTByIH7N	M1nJVFI1KGh?	NFXFdFdFVVORwrC=	M{nkPZNpd3e\|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25?	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
SK-NEP-1	MonsSpVv[3Srb36gRZN{[Xl?	NWPqc\|RrPTBxMUCwJI5O	NVXDNY55OjRiaB?=	NGCyeYRFVVORwrC=	NYCxSYh6cW6mdXPld{Bk\WyuIHP5Z4xmKGSrc3;y[IVzyqB?	NIP1OZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO\|YzOTl:L3G+
G401	NY\w[JBiTnWwY4Tpc44hSXO\|YYm=	MXy1NE8yODBibl2=	M3SzRlI1KGh?	NV;ySo1CTE2VT9Mg	MX7pcoR2[2W\|IHPlcIwh[3mlbHWg[Il{d3KmZYNCpC=>	NFW2SHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO\|YzOTl:L3G+
RPMI 8226	Mlj3R4VtdCCVdYL2bZZidCCDc4PhfS=>	NHPvNGkzNzRxNjDuUS=>	Mnv0OFjjiImq		MX7pcoR2[2W\|IHGgd4lodmmoaXPhcpQh\GWlcnXhd4UhcW5idHjlJINmdGxiZ4Lve5Rp	Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
OPM2	MUPD[YxtKFO3co\peoFtKEG\|c3H5	NWPBWJpCOi92L{[gcm0>	NVrFRnVtPDkkgJno		NHX6boVqdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq	NVfNUIRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
U266	NX[zeoZRS2WubDDTeZJ3cX[jbDDBd5NigQ>?	MWeyM\|QwPiCwTR?=	M{L6W\|Q56oDLaB?=		M3S5c4lv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh?	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
H929	M{O2emNmdGxiU4Xyeol3[WxiQYPzZZk>	MkXkNk81NzZibl2=	MkOwOFjjiImq		MYXpcoR2[2W\|IHGgd4lodmmoaXPhcpQh\GWlcnXhd4UhcW5idHjlJINmdGxiZ4Lve5Rp	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
RPMI 8226	MVzBdI9xfG:\|aYOgRZN{[Xl?	MlH4OQKBkW6P	MVWyOE81QCCq		MVvpcoR2[2W\|IHPlcIwh[XCxcITvd4l{KGmwIHGgeIlu\S2mZYDlcoRmdnRibXHucoVz	MnWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
HCC827	NIDKVXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MljrNVAhdk1?	MYC0PEBp	NW\Ie2tqTE2VTx?=	M{XxT4VvcGGwY3XzJINqe3CuYYTpckB{\W6\|aYTpeol1gcLi	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl2NE[xO{c,OjV7NES2NVc9N2F-
NCI-H23	NYX5XGRtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmfpNVAhdk1?	MmnHOFghcA>?	NHHvXJlFVVOR	M3;nNYVvcGGwY3XzJINqe3CuYYTpckB{\W6\|aYTpeol1gcLi	NH21V4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0OFYyPyd-MkW5OFQ3OTd:L3G+
AML3	NESwRllHfW6ldHnvckBCe3OjeR?=	NFXnXIMxNTFizszN	NIP2T5AzPMLiaB?=		MnfTbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK=	NUC0PZE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
ML-1	M16zc2Z2dmO2aX;uJGF{e2G7	MmXWNE0yKM7:TR?=	NETGTnkzPMLiaB?=		MlTIbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK=	NVq2cVVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
RPMI-8226vr10	M1r1PGZ2dmO2aX;uJGF{e2G7	NFzHOW0xNTFizszN	NXzvcJd6OjUEoHi=		NV\pdnplcW6mdXPld{BFVkFiZoLh[41mdnSjdHnvcuKhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI>	M1L6[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
ML-1	NEjQNJhHfW6ldHnvckBCe3OjeR?=	MXqxJO69VQ>?	M2jTOFI1yqCq		NVjnU2RZcW6lcnXhd4V{KGOjc4Dhd4UuOyCjY4Tpeol1gSB2LX\vcIQ>	M{\3eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
RPMI-8226vr10	NUTUNphMTnWwY4Tpc44hSXO\|YYm=	MlPmNUDPxE1?	M1\FbFI1yqCq		NIfDRWhqdmO{ZXHz[ZMh[2G\|cHHz[U0{KGGldHn2bZR6KDJwNT3mc4xl	M3H2N\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
SK-N-BE (2)	NHT0RpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MnexNlTjiImq		MXHJR\|UxRTFyND6w5qCKyrIkgJm3Mlghdk1?	NIXVTW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), PAN MK	NVTtXJhKT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M1n6O\|I16oDLaB?=		NHXJdVRKSzVyPUGwOE4x6oDLwsJihKk4Njhibl2=	Ml7SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV\|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-BE (2), MK PAN	NYXBZ\|R3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M4TQSVI16oDLaB?=		MWnJR\|UxRTN6Mj6w5qCKyrIkgJm0N{4zKG6P	NHTjdFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-AS	MlvKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NUn4SWR3OjUkgJno		MnuyTWM2OD1\|Nz6x5qCKyrIkgJmyMlQhdk1?	NX3GeW9zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N\|A5QTF4PD;hQi=>
SK-N-DZ	MnG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MXSyOQKBkWh?		NYnmN5RSUUN3ME2xO{4y6oDLwsJihKkxNjRibl2=	NG\WT449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
Caki-1	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoD4NVAwOjVxNUCgcm0>	NV3lfGEyPDhiaB?=		NInvTldqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meiC\|eX7ldodqe3SrY3HscJkhf2m2aDDybZRwdmG4aYK=	NYDWSJRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO\|kyQTFpPkK1Nlc6OTlzPD;hQi=>
ACHN	NEjMWHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4XHeVExNzJ3L{WwJI5O	MYW0PEBp		NIjrXIdqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meiC\|eX7ldodqe3SrY3HscJkhf2m2aDDybZRwdmG4aYK=	MlLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{mxPVEoRjJ3Mke5NVkyRC:jPh?=
769-P	NHPoO5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUCxNE8zPS93MDDuUS=>	MWK0PEBp		NVr5UYdrcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJic4nu[ZJocXO2aXPhcIx6KHerdHigdol1d26jdnny	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5OUG5NUc,OjV{N{mxPVE9N2F-
786-O	M{i0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MX:xNE8zPS93MDDuUS=>	MVO0PEBp		NWTHZ\|JQcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJic4nu[ZJocXO2aXPhcIx6KHerdHigdol1d26jdnny	MkLDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{mxPVEoRjJ3Mke5NVkyRC:jPh?=
Caki-1	NIDWfY1CeG:ydH;zbZMhSXO\|YYm=	MX:1NEBvVQ>?	M{\id\|Q5KGh?		NUHTNYNvcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDjc41jcW6nZDDybZRwdmG4aYK=	NEnrUZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PVE6OSd-MkWyO\|kyQTF:L3G+
ACHN	NFnRdlRCeG:ydH;zbZMhSXO\|YYm=	M2fLdlUxKG6P	NX3hZ2NMPDhiaB?=		MWPpcoR2[2W\|IHPlcIwh[XCxcITvd4l{KGOxbXLpcoVlKHKrdH;uZZZqeg>?	NWfIUpE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO\|kyQTFpPkK1Nlc6OTlzPD;hQi=>
769-P	NWXPPW1PSXCxcITvd4l{KEG\|c3H5	Ml7yOVAhdk1?	NGrlfVg1QCCq		M1G0XYlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3;tZolv\WRicnn0c45ifmm{	M3ro[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke5NVkyLz5{NUK3PVE6OTxxYU6=
786-O	MlTHRZBweHSxc3nzJGF{e2G7	M{C3b\|UxKG6P	NYrUV2NUPDhiaB?=		MXLpcoR2[2W\|IHPlcIwh[XCxcITvd4l{KGOxbXLpcoVlKHKrdH;uZZZqeg>?	NEG5ZnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PVE6OSd-MkWyO\|kyQTF:L3G+
Caki-1	NX7GSYJlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUfDRnBjOjVxNUCgcm0>	MXy0PEBp	MmTpSG1UVw>?	M2OxVIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVzKHO7bnXy[4l{fGmlYXzsfUB4cXSqIHLvdpRmgm:vaXK=	NX3PZW5vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxO\|Y{PTRpPkK1NVc3OzV2PD;hQi=>
ACHN	M{HMRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NV\VO\|V{OjVxNUCgcm0>	MXW0PEBp	NIrz[oNFVVOR	NYPhPVc3cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJic4nu[ZJocXO2aXPhcIx6KHerdHigZo9zfGW8b33pZi=>	NXfCWoZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxO\|Y{PTRpPkK1NVc3OzV2PD;hQi=>
769-P	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH\BT3IzPS93MDDuUS=>	MV60PEBp	M1u4TGROW09?	Ml\RbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZIhe3mwZYLnbZN1cWOjbHz5JJdqfGhiYn;yeIV7d22rYh?=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
Caki-1	NWHLWJlyS2:ub375JGZwem2jdHnvckBCe3OjeR?=	MmDZOVAhdk1?	MY[3MVE1KGR?	NW[2eYhkTE2VTx?=	NUHPS25Te3WycILld5Nm\CClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bImgZ49u[mmwZXSge4l1cCC5aYToJIJwenSnen;tbYIhyqB?	NGXO[II9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG3OlM2PCd-MkWxO\|Y{PTR:L3G+
ACHN	NULKVmZXS2:ub375JGZwem2jdHnvckBCe3OjeR?=	MWC1NEBvVQ>?	NIrVT4c4NTF2IHS=	MUfEUXNQ	NWrweWg4e3WycILld5Nm\CClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bImgZ49u[mmwZXSge4l1cCC5aYToJIJwenSnen;tbYIhyqB?	M3POXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUe2N\|U1Lz5{NUG3OlM2PDxxYU6=
769-P	MXLDc4xwdnliRn;ycYF1cW:wIFHzd4F6	Mli0OVAhdk1?	MWi3MVE1KGR?	NXjjbVkzTE2VTx?=	NYnOXpBNe3WycILld5Nm\CClb3zvcpkh\m:{bXH0bY9vKHOrZ37p[olk[W62bImgZ49u[mmwZXSge4l1cCC5aYToJIJwenSnen;tbYIhyqB?	NFrSTWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG3OlM2PCd-MkWxO\|Y{PTR:L3G+
Caki-1	M125RWFxd3C2b4Ppd{BCe3OjeR?=	M1S2cFUxKG6P	NXrrOWx6PDhiaB?=	NYX2eIl5TE2VTx?=	M3;q[Ylv\HWlZYOgZ4VtdCCjcH;weI9{cXN?	Ml3yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzN{[zOVQoRjJ3MUe2N\|U1RC:jPh?=
ACHN	M{e1SmFxd3C2b4Ppd{BCe3OjeR?=	NYfrUoloPTBibl2=	NGXMPXA1QCCq	NVWwbY1nTE2VTx?=	NYD1W5RWcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
769-P	M2TufWFxd3C2b4Ppd{BCe3OjeR?=	MXG1NEBvVQ>?	MYi0PEBp	Mk\iSG1UVw>?	NXHLSlRTcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
MDA-MB-231	M3W5RW1wenCqb3zv[4lk[WxiQ4L5d5RidCCYaX;s[ZQhMEOYKTDBd5NigQ>?	MmLGNVDDqG6P	MkfON:Kh\A>?	MXfEUXNQ	MmHCZYx1\XK\|IHPlcIwhdW:{cHjvcI9ogcLi	M4LyXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEGwOFk4Lz5{NEixNFQ6PzxxYU6=
BT-549	NF[xZ2FOd3KyaH;sc4dq[2GuIFPyfZN1[WxiVnnvcIV1KCiFVjmgRZN{[Xl?	MnW3NVDDqG6P	Ml7hN:Kh\A>?	M{i0eGROW09?	NXK5fHdF[Wy2ZYLzJINmdGxibX;ydIhwdG:pedMg	M3;TZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEGwOFk4Lz5{NEixNFQ6PzxxYU6=
MCF-7	NFz3bWJOd3KyaH;sc4dq[2GuIFPyfZN1[WxiVnnvcIV1KCiFVjmgRZN{[Xl?	MlPmNVDDqG6P	NHXQdlM{yqCm	MYfEUXNQ	MUDhcJRmenNiY3XscEBud3KyaH;sc4d6yqB?	NWLzZZc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVA1QTdpPkK0PFExPDl5PD;hQi=>
MCF-7	NHTuWIdHfW6ldHnvckBCe3OjeR?=	NYLWS\|J2PS13MDDuUS=>	MlPtNlQhcA>?	MmiySG1UVw>?	M2DhOZJm\HWlZXSgeIhmKGyndnXsJI9nKGW6cILld5Nqd25ib3[gSXLPuSxiUGKgZY5lKE[xeFGxxsA>	NGX0fIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2OlQxPyd-MkSzOlY1ODd:L3G+
CTS	MnHYRZBweHSxc3nzJGF{e2G7	MV[w5qCUPDBibl5CpC=>	NYTwRXJqPDhiaB?=		MVfpcoR2[2W\|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NESyPUc,OjR{NES0Nlk9N2F-
OCI-AML3	MoPqRZBweHSxc3nzJGF{e2G7	NHvxR5Mx6oDVNECgcm3DqA>?	MkX6OFghcA>?		M4DpUolv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	NXPkZnF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQ1OjlpPkK0NlQ1PDJ7PD;hQi=>
U937	M1K0dGFxd3C2b4Ppd{BCe3OjeR?=	Mlm1NQKBmzRyIH7NxsA>	NGrqcXU1QCCq		M4q1c4lv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NESyPUc,OjR{NES0Nlk9N2F-
PC3	MmDGRZBweHSxc3nzJGF{e2G7	NWrNcVVKOC1zMECgcm0>	M2HqV\|I1NzR6IHi=		NWDLU3V1cW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI>	M1SxO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[zNlMxLz5{NEG2N\|I{ODxxYU6=
PC3-AR	NETrb3ZCeG:ydH;zbZMhSXO\|YYm=	MmPVNE0yODBibl2=	NIPSSGczPC92ODDo		NIXZephqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ?	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4M{KzNEc,OjRzNkOyN\|A9N2F-
PC3	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M{HCe\|AuOTByIH7N	M2iwT\|I1NzR6IHi=		MXzpcoR2[2W\|IHHjZ5VufWyjdHnvckBw\iC\|dXLHNUBxd3C3bHH0bY9v	M1XhN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[zNlMxLz5{NEG2N\|I{ODxxYU6=
PC3-AR	NXHVS2QxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NYLnRZE3OC1zMECgcm0>	NFGwRmgzPC92ODDo		MoTtbY5lfWOnczDj[YxtKGO7Y3zlJIFzemW\|dDDpckB1cGViR{LNJJBp[XOn	MlftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNkOyN\|AoRjJ2MU[zNlMxRC:jPh?=
PC3	NWHSdWk6TnWwY4Tpc44hSXO\|YYm=	M4LKNlAuOTByIH7N	MWiyOEBp		Mmfzd5VxeHKnc4Pld{BmgHC{ZYPzbY9vKG:oIHHjeIl3[XSnZDDBWG0tKEGtdDDhcoQhTXKtMT:yJJBzd3SnaX6=	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4M{KzNEc,OjRzNkOyN\|A9N2F-
PC3-AR	NH3nZZJHfW6ldHnvckBCe3OjeR?=	NInsRnoxNTFyMDDuUS=>	MX2yOEBp		MoPId5VxeHKnc4Pld{BmgHC{ZYPzbY9vKG:oIHHjeIl3[XSnZDDBWG0tKEGtdDDhcoQhTXKtMT:yJJBzd3SnaX6=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4M{KzNEc,OjRzNkOyN\|A9N2F-
OS-RC-2	M1noRWNmdGxiVnnhZoltcXS7IFHzd4F6	MkHyNE0yODByIH7N	MnzaNlQwPDhxN{KgbC=>	M1jHc2ROW09?	NWXCdoVE\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGKxdHigeIlu\S1iYX7kJIRwe2VvZHXw[Y5l\W62IH3hco5meg>?	NHywdVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0OFc{Pyd-MkSxOFQ4Ozd:L3G+
OS-RC-2	M2XIUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{nLfVUxKG6P	M2fDd\|Q5KGh?	NIDGXWtFVVOR	MVjpcoR2[2W\|IFeyM20h[XK{ZYP0	NUPLNnN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFQ4OzdpPkK0NVQ1PzN5PD;hQi=>
OS-RC-2	MXzBdI9xfG:\|aYOgRZN{[Xl?	MoPuOVAhdk1?	MXe0PEBp	NHPHTo1FVVOR	MVHpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	M3XkW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS0O\|M4Lz5{NEG0OFc{PzxxYU6=
SK-N-AS	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYHxeHFsOOLCk{iwJI5O	Mmf4OFghcA>?		M3PZd2lEPTB;MkeuOEBvVQ>?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEe5PUc,OjRyOUi3PVk9N2F-
SK-N-DZ	NGH2OGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVWw5qCUQDBibl2=	NYXHV2pPPDhiaB?=		M{PDdGlEPTB;MkGuPUBvVQ>?	NX;jSGFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg4QTlpPkK0NFk5Pzl7PD;hQi=>
SK-N-SH	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWqw5qCUQDBibl2=	M{WyVFQ5KGh?		MXfJR\|UxRTd{LkOgcm0>	NULPV41mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg4QTlpPkK0NFk5Pzl7PD;hQi=>
SK-N-BE	NVP0T3BxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MkS5NQKBmzhyIH7N	M1XDO\|Q5KGh?		NUj3RnFDUUN3ME23OU41KG6P	MmXoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O\|k6RC:jPh?=
SK-N-AS	MVjBdI9xfG:\|aYOgRZN{[Xl?	NHHuWoox6oDVOECgcm0>	MUi0PEBp		NEP4dplxd3SnboTsfUBqdmS3Y3XkJIFxd3C2b4Ppd{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG[jc3jpc44>	NIjwRmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5PFc6QSd-MkSwPVg4QTl:L3G+
SK-N-DZ	M3joc2Fxd3C2b4Ppd{BCe3OjeR?=	NHfM[44x6oDVOECgcm0>	M1KzcVQ5KGh?		NXzpZZZReG:2ZX70cJkhcW6mdXPl[EBieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCoYYPobY9v	NIjLR3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5PFc6QSd-MkSwPVg4QTl:L3G+
SK-N-SH	M2DjWmFxd3C2b4Ppd{BCe3OjeR?=	M{ixZ\|DjiJN2MDDuUS=>	M3WwPVQ5KGh?		M3XMepBwfGWwdHz5JIlv\HWlZXSgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQh\mG\|aHnvci=>	M1f6T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm4O\|k6Lz5{NEC5PFc6QTxxYU6=
SK-N-BE	MluxRZBweHSxc3nzJGF{e2G7	MViw5qCUPDBibl2=	M3\UXVQ5KGh?		NWjz[VlSeG:2ZX70cJkhcW6mdXPl[EBieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCoYYPobY9v	MkO1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O\|k6RC:jPh?=
SK-N-AS	NVPjNYs4TnWwY4Tpc44hSXO\|YYm=	MUGw5qCUQDBibl2=	M2i2PFQ5KGh?		MWjpcoR2[2W\|IHGg[I9{\S2mZYDlcoRmdnRiY3zlZZZi\2Vib3[gZ4F{eGG\|ZTCzJIFv\CCSQWLQ	Ml7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O\|k6RC:jPh?=
SK-N-DZ	MYHGeY5kfGmxbjDBd5NigQ>?	MWiw5qCUQDBibl2=	NHrKVGo1QCCq		MVTpcoR2[2W\|IHGg[I9{\S2mZYDlcoRmdnRiY3zlZZZi\2Vib3[gZ4F{eGG\|ZTCzJIFv\CCSQWLQ	NHu4cHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5PFc6QSd-MkSwPVg4QTl:L3G+
SK-N-SH	NGDqS2FHfW6ldHnvckBCe3OjeR?=	NFHuOmYx6oDVNECgcm0>	MUm0PEBp		NI[yUlFqdmS3Y3XzJIEh\G:\|ZT3k[ZBmdmSnboSgZ4xm[X[jZ3Wgc4Yh[2G\|cHHz[UA{KGGwZDDQRXJR	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEe5PUc,OjRyOUi3PVk9N2F-
SK-N-BE	MU\GeY5kfGmxbjDBd5NigQ>?	MVqw5qCUPDBibl2=	M1vrRVQ5KGh?		NG[2WmZqdmS3Y3XzJIEh\G:\|ZT3k[ZBmdmSnboSgZ4xm[X[jZ3Wgc4Yh[2G\|cHHz[UA{KGGwZDDQRXJR	NHTYNlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5PFc6QSd-MkSwPVg4QTl:L3G+
HCC-LM3	NGj3SmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NXO0[GwxOS1zMECwJI5O	MUSyOE81QC95MjDo	M1rHRmROW09?	M3\nPIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	M2S2R\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N\|k2PjxxYU6=
HepG2	M{nubGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYj4PFZIOS1zMECwJI5O	MVqyOE81QC95MjDo	Ml\zSG1UVw>?	MYDpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ?	M3zPW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N\|k2PjxxYU6=
SMMC-7721	NHTaRYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MV[xMVExODBibl2=	MlfyNlQwPDhxN{KgbC=>	NHiw[5dFVVOR	M2XuNYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	NIfwNlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N\|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3	NIPDVIVCeG:ydH;zbZMhSXO\|YYm=	MV:1NEBvVQ>?	M1O2VVQ5KGh?	MonVSG1UVw>?	MYjpcoR2[2W\|IHPlcIwh[XCxcITvd4l{KHOrZ37p[olk[W62bImgbY4h[SClYYPwZZNmNWSncHXu[IVvfCCvYX7u[ZIh[nliY3zlZZZi\2Vib3[gZ4F{eGG\|ZYOgN{whQCCjbnSgPS=>	MoXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HepG2	MULBdI9xfG:\|aYOgRZN{[Xl?	NVvxTlNyPTBibl2=	M1vzflQ5KGh?	MVzEUXNQ	NWrVU\|B2cW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzbYdvcW[rY3HueIx6KGmwIHGgZ4F{eGG\|ZT3k[ZBmdmSnboSgcYFvdmW{IHL5JINt\WG4YXflJI9nKGOjc4Dhd4V{KDNuIEigZY5lKDl?	M4rQOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N\|k2PjxxYU6=
SMMC-7721	MYjBdI9xfG:\|aYOgRZN{[Xl?	NHfWToI2OCCwTR?=	MkXWOFghcA>?	NVfWWlVZTE2VTx?=	NUPTZWhrcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzbYdvcW[rY3HueIx6KGmwIHGgZ4F{eGG\|ZT3k[ZBmdmSnboSgcYFvdmW{IHL5JINt\WG4YXflJI9nKGOjc4Dhd4V{KDNuIEigZY5lKDl?	NF\rVVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N\|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3	Mn3SSpVv[3Srb36gRZN{[Xl?	M{CxeVUxNzFyMDDuUS=>	MmGyNlQhcA>?	NHvGWHVFVVOR	M4PIPYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC=	MkXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HepG2	MV\GeY5kfGmxbjDBd5NigQ>?	MojmOVAwOTByIH7N	MmnKNlQhcA>?	NF3JfodFVVOR	M1zqOYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC=	MoT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721	MkHwSpVv[3Srb36gRZN{[Xl?	MV21NE8yODBibl2=	MWSyOEBp	MlH3SG1UVw>?	MlHU[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicD3TWGFVOyCjbnSgdE1Cc3UEoB?=	NIO3U4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N\|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3	M4XUR2Z2dmO2aX;uJGF{e2G7	MW[1NE8yODBibl2=	NYDHbZg1OjRiaB?=	NYPhbXFiTE2VTx?=	MYPkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v	NEPab449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N\|k2Pid-MkSwPVM6PTZ:L3G+
HepG2	MW\GeY5kfGmxbjDBd5NigQ>?	NGL6SHM2OC9zMECgcm0>	NIDhd5gzPCCq	MV;EUXNQ	NEO0V4Vld3ewcnXneYxifGW\|IFLjcE15VCCneIDy[ZN{cW:w	M4jWXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N\|k2PjxxYU6=
SMMC-7721	NHvPUHRHfW6ldHnvckBCe3OjeR?=	M2r1SlUxNzFyMDDuUS=>	MYiyOEBp	NUn4b3VyTE2VTx?=	MXHkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v	NIXB[\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N\|k2Pid-MkSwPVM6PTZ:L3G+
FaDu	M2rpT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MoLlNVAx6oDHbl2=	NUW1Z4J4QC9zMD:xNkBp		M1fUfoRqe3CuYYnl[EBiKHOrZ37p[olk[W62IHHu[EBxem:ub37n[YQhTzJxTTDhdpJme3RiYYSgPEBidmRiMUNihKVpKHCxc4SgdoVt\WG\|ZR?=	NFL0OI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyOlQ5Oid-MkSwNlY1QDJ:L3G+
FaDu	NWL5S4xxTnWwY4Tpc44hSXO\|YYm=	NHXuSlEyODEkgJXuUS=>	M{i4O\|IwPC96L{GyJIg>		M4PnfIlv\HWlZXSgdFIyX2GoMT;DbZAyyqCneIDy[ZN{cW:w	M1u1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK2OFgzLz5{NECyOlQ5OjxxYU6=
PC-3	M{nXOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1LaO\|AuOTBizszN	MVKyOE81QC95MjDo		M3\QeYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	NUXNbFUzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PVEzOTZpPkKzPVkyOjF4PD;hQi=>
LNCaP	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mnr5NE02KM7:TR?=	M2XJXlI1NzR6L{eyJIg>		MXPpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ?	M1O3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUmxNlE3Lz5{M{m5NVIyPjxxYU6=
RWPE-1	NEfRZo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVPsdXdjOC1{MDFOwG0>	M3jWfVI1NzR6L{eyJIg>		NFftXZFqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK=	M3O1d\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUmxNlE3Lz5{M{m5NVIyPjxxYU6=
Capan-1	NVjV[ldWTnWwY4Tpc44hSXO\|YYm=	NFv0PWQzPS93MD:xNFAhdk1?	MXG4M\|I1NzR6IHi=	NVKwR5p{TE2VTx?=	NHHlTFZld3ewcnXneYxifGWmIGLvckBuWk6DIHHu[EBxem:2ZXnuJIV5eHKnc4Ppc44h[W6mIHTve45{fHKnYX2gd4lodmGuaX7n	NGDNTnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{myNlg5Pid-MkO5NlI5QDZ:L3G+
L3.6pl	NXrybWlQTnWwY4Tpc44hSXO\|YYm=	MnftNlUwPTBxMUCwJI5O	M3XiVFgwOjRxNEigbC=>	NG\Ze3ZFVVOR	MUfkc5dvemWpdXzheIVlKFKxbjDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36gZY5lKGSxd37zeJJm[W1ic3nncoFtcW6p	M{TrT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUKyPFg3Lz5{M{myNlg5PjxxYU6=
CFPAC-1	MYHGeY5kfGmxbjDBd5NigQ>?	NHnrW5IzPS93MD:xNFAhdk1?	MoDzPE8zPC92ODDo	MlrVSG1UVw>?	MnvW[I94dnKnZ4XsZZRm\CCUb36gcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO\|aX;uJIFv\CCmb4fud5Rz\WGvIIPp[45idGmwZx?=	Mly4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ\|OUKyPFg3RC:jPh?=
Capan-1	NWXuUmg{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MlPRNlUwPTBxMUCwJI5O	NVnJdZF6PDhiaB?=	M{fQSmROW09?	NGTOeFFz\WS3Y3XzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz	NUSwSWJKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
L3.6pl	NY\ld2RZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{\XfFI2NzVyL{GwNEBvVQ>?	NH3FclE1QCCq	NFvkXnRFVVOR	Mm\wdoVlfWOnczDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>	NF;Hd2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{myNlg5Pid-MkO5NlI5QDZ:L3G+
CFPAC-1	NEXsUnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NXXobXdSOjVxNUCvNVAxKG6P	NFnMc4E1QCCq	Ml\rSG1UVw>?	NYraN2tSemWmdXPld{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>?	NYL6fFA6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
Capan-1	M2S1cmFxd3C2b4Ppd{BCe3OjeR?=	NXPJPG0zOjVxNUCvNVAxKG6P	M4nFdFQ5KGh?	MWrEUXNQ	MYTpcoR2[2W\|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl{Mki4Okc,OjN7MkK4PFY9N2F-
L3.6pl	MX7BdI9xfG:\|aYOgRZN{[Xl?	M33sUFI2NzVyL{GwNEBvVQ>?	Mn3NOFghcA>?	NFS0OFNFVVOR	MYHpcoR2[2W\|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	MkPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ\|OUKyPFg3RC:jPh?=
CFPAC-1	M1HYO2Fxd3C2b4Ppd{BCe3OjeR?=	Mn72NlUwPTBxMUCwJI5O	NWT0bI8xPDhiaB?=	NYrkfIR4TE2VTx?=	Mn3xbY5lfWOnczDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl{Mki4Okc,OjN7MkK4PFY9N2F-
HN22	Mn\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NY\u[Xc6OC1{MDDuUS=>	Mm[2NlQwPDhiaB?=	M3XCbmROW09?	MVHpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMUBl\XCnbnTlcpQhdWGwbnXy	MkHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{eyN\|UoRjJ\|OEe3NlM2RC:jPh?=
HSC4	MlvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXnzZ4QxOC1{MDDuUS=>	Ml72NlQwPDhiaB?=	MYDEUXNQ	MVXpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMUBl\XCnbnTlcpQhdWGwbnXy	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN\|U9N2F-
HN22	M4DoUmFxd3C2b4Ppd{BCe3OjeR?=	M2\TSVAuOjBibl2=	MlHyOFghcA>?	NUO1PWd4TE2VTx?=	NXLlN3BXcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN\|U9N2F-
HSC4	NF[wXpNCeG:ydH;zbZMhSXO\|YYm=	M3\jflAuOjBibl2=	M3X6ZlQ5KGh?	M1XlcWROW09?	MoDEbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	M1npbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEe3NlM2Lz5{M{i3O\|I{PTxxYU6=
HN22	MlnNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MXewMVIxKG6P	NGHwdYU1QCCq	MkHlSG1UVw>?	NVO1e2N4cW6mdXPld{BIOSCyaHHz[UBk\WyuIHP5Z4xmKGG{cnXzeOKh	NWTMSWFGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O\|czOzVpPkKzPFc4OjN3PD;hQi=>
HSC4	NXXqXJFtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MnrONE0zOCCwTR?=	NGrIbYQ1QCCq	M4i2fGROW09?	NUT0eHdncW6mdXPld{BIOSCyaHHz[UBk\WyuIHP5Z4xmKGG{cnXzeOKh	NHKzS2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3O\|I{PSd-MkO4O\|czOzV:L3G+
HN22	MkPwSpVv[3Srb36gRZN{[Xl?	NF2wT3UxNTJyIH7N	MoP5OFghcA>?	MmPZSG1UVw>?	NYXISWRbe3WycILld5NmeyCVcEGg[ZhxemW\|c3nvcuKh	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN\|U9N2F-
HSC4	M4Kwe2Z2dmO2aX;uJGF{e2G7	NUTsTmdbOC1{MDDuUS=>	MmfaOFghcA>?	MkO0SG1UVw>?	NH3HOmR{fXCycnXzd4V{KFOyMTDlfJBz\XO\|aX;uxsA>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN\|U9N2F-
Cal62	NXPNdZV{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnrnTWM2OD1\|MzFCtUA1KG6P	NGDNWYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOFA3PCd-MkO4NlQxPjR:L3G+
Hth7	NVu0UndUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NX\tS2FYUUN3ME2xOUDDuSB{IH7N	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC2OEc,OjN6MkSwOlQ9N2F-
Hth83	MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVnBS3FGUUN3ME2zOEDDuSB3IH7N	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC2OEc,OjN6MkSwOlQ9N2F-
C643	NH;leWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NI\wSXdKSzVyPUexJOKyKDFyIH7N	NEC0Um49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOFA3PCd-MkO4NlQxPjR:L3G+
SW1736	NH3XcYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1y5b2lEPTB;M{WgxtEhQCCwTR?=	M1XwV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEK0NFY1Lz5{M{iyOFA3PDxxYU6=
T241	MoDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFvUcVJKSzVyPU[1JOKyKDdibl2=	NUXxdVV3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlQxPjRpPkKzPFI1ODZ2PD;hQi=>
T351	MlfTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MU\JR\|UxRTVyINMxJFExKG6P	NET0TWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOFA3PCd-MkO4NlQxPjR:L3G+
BHP2-7	NHjFdWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlXvTWM2OD1\|NzFCtUA3KG6P	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC2OEc,OjN6MkSwOlQ9N2F-
T238	M3\Wb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MkPjTWM2OD1zLEWwNEDDuSB{MECgcm0>	NFLtOJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOFA3PCd-MkO4NlQxPjR:L3G+
HCT8	NEjTfnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		M13IRlczKGh?	M2PSfGROW09?	NHSzUGpKSzVyPUGyMlnjiIoEsfMAjVEvQSCwTR?=	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7OUO4PEc,OjN{OUmzPFg9N2F-
H630	NHf3TWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NIPVdI04OiCq	NFXTUXVFVVOR	M17mXWlEPTB;MUKuOQKBkcLz4pEJN{4yKG6P	M{\lVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mkm5N\|g5Lz5{M{K5PVM5QDxxYU6=
cH630 5-FU-res	M1e0OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NELuTmg4OiCq	Moe1SG1UVw>?	NIjFdoRKSzVyPUG1MlXjiIoEsfMAjVEvOiCwTR?=	NYPiZVBERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPVk{QDhpPkKzNlk6Ozh6PD;hQi=>
HCT116	M1;HZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		Mn;1O\|IhcA>?	NHPWRm9FVVOR	Mnq0TWM2OD1zMD635qCKyrIkgJmyMlIhdk1?	NFvlRW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
HCT116 p53−/−	NFG0O5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NFi0S2s4OiCq	NGiwclRFVVOR	MlPOTWM2OD16LkdihKnDueLCiUGuO{BvVQ>?	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7OUO4PEc,OjN{OUmzPFg9N2F-
dHCT116 p21−/−	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MnLDO\|IhcA>?	NF33folFVVOR	M3vVUGlEPTB;NT655qCKyrIkgJmxMlMhdk1?	NHH6fnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
HT29	M{DQ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NYHqVlhtPzJiaB?=	NGHUNW1FVVOR	M{TJSWlEPTB;MU[uN-KBkcLz4pEJNk4{KG6P	NI\SOGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
LoVo	NGLpWnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NUnpRZd7PzJiaB?=	MX7EUXNQ	M3LPbmlEPTB;NT6x5qCKyrIkgJmwMlYhdk1?	NGXUTmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
RKO	MnnWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MonoO\|IhcA>?	NYXKRW5PTE2VTx?=	NEfRRpVKSzVyPUeuPgKBkcLz4pEJNk4zKG6P	M3PxSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mkm5N\|g5Lz5{M{K5PVM5QDxxYU6=
SW480	NF\lcVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NHPhUpo4OiCq	NFq2XG1FVVOR	MWHJR\|UxRTF5LkZihKnDueLCiUCuPEBvVQ>?	NXTyOVFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPVk{QDhpPkKzNlk6Ozh6PD;hQi=>
eSW620	NYTyTmtCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NWP3WpdUPzJiaB?=	M1fZ[WROW09?	MX7JR\|UxRTlwMfMAjeKy6oDLMj6xJI5O	M2LsRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mkm5N\|g5Lz5{M{K5PVM5QDxxYU6=
S2	M3T4WmZ2dmO2aX;uJIF{e2G7				MWrJcohq[mm2aX;uJI9nKFCuYYPtc4RqfW1iZnHsZ4lx[XK3bTDISGFEOSCneIDy[ZN{\WRiaX6gSJJwe2:yaHnsZUBu\Wyjbn;nZZN1\XJiU{KgZ4VtdHNuIFnDOVAhRSByLkCwNVgh\|ryPLh?=	NGPNTVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOxO\|Q2OCd-MUmzNVc1PTB:L3G+
COLO205	MmX0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M2rOV\|k3KGi{cx?=		MnPZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCFT1zPNlA2KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDj[YxtfGm2ZYKgPVYh[XO\|YYmsJGlEPTBiPTCwMlAyQCEQvF2u	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ\|NESzNEc,OjF4M{S0N\|A9N2F-
PC3	NUfvU5FLSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MUi5OkBpenN?		NEfVV\|BCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDh[pRmeiB7NjDodpMh[nliY3XscJRqfGW{IEm2JIF{e2G7LDDJR\|UxKD1iMD6wNlQh\|ryPLh?=	M{XmflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkO0OFMxLz5{MU[zOFQ{ODxxYU6=
A2780	NV;RXndsSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NFX5Vmg6PiCqcoO=		NHvBXYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGyO\|gxKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDj[YxtfGm2ZYKgPVYh[XO\|YYmsJGlEPTBiPTCwMlA{PSEQvF2u	M2DwfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkO0OFMxLz5{MU[zOFQ{ODxxYU6=
HCT116	MW\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NX3IcpdFQTZiaILz		MnTyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiOU[gbJJ{KGK7IHPlcIx1cXSncjC5OkBie3OjeTygTWM2OCB;IECuNFQ5KM7:TT6=	M2ri[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkO0OFMxLz5{MU[zOFQ{ODxxYU6=
HEK293	NGnXZWJHfW6ldHnvckBie3OjeR?=				MlHWTY5pcWKrdHnvckBw\iCKRFHDN{ApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgeZNqdmdiW{PIYYFk\XS7bHH0[YQhcHWvYX6gbIl{fG:wZTDIOEBx\XC2aXTlJIF{KHO3YoP0doF1\SCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCLQ{WwJF0hOC5yMEKxJO69VS5?	NX\0dXBkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOFQ4ODFpPkKyN\|Q1PzBzPD;hQi=>
HEK293	M1HpUmZ2dmO2aX;uJIF{e2G7				NYG3THdRUW6qaXLpeIlwdiCxZjDISGFEOSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMhfXOrbnegX\|NJZWGlZYT5cIF1\WRiaIXtZY4hcGm\|dH;u[UBJPCCyZYD0bYRmKGG\|IIP1ZpN1emG2ZTDifUB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDJR\|UxKD1iMD6wNFI2KM7:TT6=	NIDhdJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO0OFcxOSd-MkKzOFQ4ODF:L3G+
HEK293	MUDGeY5kfGmxbjDhd5NigQ>?				Mle0TY5pcWKrdHnvckBw\iCKRFHDOkApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgeZNqdmdiW{PIYYFk\XS7bHH0[YQhcHWvYX6gbIl{fG:wZTDIOEBx\XC2aXTlJIF{KHO3YoP0doF1\SCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCLQ{WwJF0hOC5yMUGg{txONg>?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2NEewNUc,OjJ\|NES3NFE9N2F-
SF21	NU\Wbo5ITnWwY4Tpc44h[XO\|YYm=				MWDJcohq[mm2aX;uJI9nKG[uYXeteIFo\2WmIFjERWMzKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIGPGNlEh[2WubIOgeZNqdmdiW{PIYYFk\XS7bHH0[YQhcHWvYX6gbIl{fG:wZTDIOEBx\XC2aXTlJIF{KHO3YoP0doF1\SCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCLQ{WwJF0hOC5yMUOg{txONg>?	MomzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|NES3NFEoRjJ{M{S0O\|AyRC:jPh?=
HEK293	MUPGeY5kfGmxbjDhd5NigQ>?				M3jBWmlvcGmkaYTpc44hd2ZiSFTBR\|QhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJJV{cW6pIGuzTH1i[2W2eXzheIVlKGi3bXHuJIhqe3SxbnWgTFQheGWydHnk[UBieyC\|dXLzeJJifGViYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiSVO1NEA:KDBwMjFOwG0v	Mlv4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|NES3NFEoRjJ{M{S0O\|AyRC:jPh?=
SF9	Mn30SpVv[3Srb36gZZN{[Xl?				NGfnXVJKdmirYnn0bY9vKG:oIHjpd{1{fHKncD30ZYdo\WRiSFTBR\|ghMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iU1[5JINmdGy\|IIXzbY5oKFt\|SG3hZ4V1gWyjdHXkJIh2dWGwIHjpd5RwdmViSESgdIVxfGmmZTDhd{B{fWK\|dILheIUh[nlic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whUUN3MDC9JFAvOjhizszNMi=>	NGnYTXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO0OFcxOSd-MkKzOFQ4ODF:L3G+
B16	MlXoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M3[0WVQ5KGi{cx?=		MVjHdo94fGhiaX7obYJqfGmxbjDv[kBud3W\|ZTDCNVYh[2WubIOgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliTWTUJIF{e2G7LDDHTVUxKD1iMD6xOUDPxE1w	NUTFNHNERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFkzODNpPkKzNFA6OjB\|PD;hQi=>
HeLa	MWLGeY5kfGmxbjDhd5NigQ>?				NXrpR2hGUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHGgZ4VtdHNidYPpcochTmy3b4Kg[IUhVHm\|IHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCzJO69VS5?	NHLiVok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[zPVU{Pyd-MkO2N\|k2Ozd:L3G+
HuH7	NGfReW9EgXSxdH;4bYNqfHliYYPzZZk>		NH7IO2E{KGSjeYO=		Mo\oR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxidnnhZoltcXS7IHHmeIVzKDNiZHH5d{BjgSCFZXzsWIl1\XJiOU[gZZN{[XluIFPDOVAhRSByLkCwN\|Uh\|ryPLh?=	MmXMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUC3NFAoRjJ3NEmwO\|AxRC:jPh?=
Sf9	MkPPSpVv[3Srb36gZZN{[Xl?		NHvX[2oyPSCvaX7z		MUDJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIFOteIVzdWmwYXygTIl{N0[OQVeteIFo\2WmIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFHjMZBmeHSrZHWtRW1EKGG\|IIP1ZpN1emG2ZTDhd5Nme3OnZDDhd{Bz\WynYYPlJI9nKEGPQzDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XO3cnXkJIEtKEmFNUCgQUAxNjByMUK2JO69VS5?	MmLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O\|YoRjJ5MUi2Olc3RC:jPh?=
Sf9	NHH2S5RHfW6ldHnvckBie3OjeR?=		NILlTY0yPSCvaX7z		MYnJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIFOteIVzdWmwYXygTIl{NXSjZ3fl[EBpfW2jbjDy[YNwdWKrbnHueEBJTEGFMz;OR29TOiBqM{m1JJRwKDR6OTDy[ZNq\HWnczmg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCCVZkmgZ4VtdHNidYPpcochSWNvcHXweIll\S2DTVOgZZMhe3Wkc4TyZZRmKGG\|c3Xzd4VlKGG\|IILlcIVie2Vib3[gRW1EKHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bnd2yub4fl[EBjgSC\|dXLzeJItKEmFNUCgQUAxNjByMkK3JO69VS5?	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O\|Y9N2F-
MV4-11	M3zufmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MlHRNlQhcHK\|		MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNWlQuOTFiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMECyPVch\|ryPLh?=	M2TmNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9	NWWzbWdzTnWwY4Tpc44h[XO\|YYm=		NVr5b2ZMOTVibXnudy=>		M2\mS2lvcGmkaYTpc44hd2ZiZoXscEBt\W6pdHigbJVu[W5icnXjc41jcW6jboSgTGRCSzJiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBU\jliY3XscJMhfXOrbnegRYMueGWydHnk[U1CVUNiYYOgd5Vje3S{YYTlJIF{e2W\|c3XkJIF{KHKnbHXhd4Uhd2ZiQV3DJJBz\WmwY4XiZZRm\CCob4KgNVUhdWmwczDmc4xtd3enZDDifUB{fWK\|dILheIUh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNUBpeiCkeTDmcJVwemW\|Y3XuZ4Uh[SxiSVO1NEA:KDBwMECzNlgh\|ryPLh?=	MkfRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O\|YoRjJ5MUi2Olc3RC:jPh?=
HCT116	MXnDfZRwfG:6aXPpeJkh[XO\|YYm=		M3nPNlI1KGi{cx?=		M2TIXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMD6wNFM{PiEQvF2u	M1rtRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9	NYfaXox2TnWwY4Tpc44h[XO\|YYm=		Ml;sNVUhdWmwcx?=		NYm3VFNjUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDy[YNwdWKrbnHueEBJTEGFNjDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiaX7z[YN1KFOoOTDj[YxteyC3c3nu[{BC[y2yZYD0bYRmNUGPQzDhd{B{fWK\|dILheIUh[XO\|ZYPz[YQh[XNicnXs[YF{\SCxZjDBUWMheHKnaX7jeYJifGWmIH\vdkAyPSCvaX7zJIZwdGyxd3XkJIJ6KHO3YoP0doF1\SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAyKGi{IHL5JIZtfW:{ZYPj[Y5k\SCjLDDJR\|UxKD1iMD6wNFQyPiEQvF2u	MmrtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O\|YoRjJ5MUi2Olc3RC:jPh?=
Sf9	M1Xw[2lvcGmkaYTpc44hd2ZiaIXtZY4>		NF\3d20yPSCvaX7z		M4DSeGlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR\|ExKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFHjMZBmeHSrZHWtRW1EKGG\|IIP1ZpN1emG2ZTDhd5Nme3OnZDDhd{Bz\WynYYPlJI9nKEGPQzDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEGgbJIh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxKD1iMD6wNFQ1PSEQvF2u	M37lXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9	M2D4PWZ2dmO2aX;uJIF{e2G7		MXWxOUBucW6\|		MojhTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDDMZRmem2rbnHsJGhqey22YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQhUESDQ{ig[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCCVZkmgZ4VtdHNidYPpcochSWNvcHXweIll\S2DTVOgZZMhe3Wkc4TyZZRmKGG\|c3Xzd4VlKGG\|IILlcIVie2Vib3[gRW1EKHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bnd2yub4fl[EBjgSC\|dXLzeJJifGViYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhNCCLQ{WwJF0hOC5yMES4OkDPxE1w	M4GxdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
A2780S	MXXDfZRwfG:6aXPpeJkh[XO\|YYm=		MV:yOEBpenN?		NH;JTFVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MGOgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFA5OzJizszNMi=>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O\|Y9N2F-
A2780S	M4K2c2Z2dmO2aX;uJIF{e2G7		M2TVWVYhcHK\|		NYPIcJZZUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCDMke4NHMh[2WubIOgZZN{\XO\|ZXSgZZMhfHWkdXzpckBi[2W2eXzheIlwdiCrbnP1ZoF1\WRiZn;yJFYhcHK\|IHL5JIN6fG:kbH;0JIF{e2G7LDDFR\|UxKD1iMD6xOVA4OSEQvF2u	MmPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O\|YoRjJ5MUi2Olc3RC:jPh?=
A2780S	NEHJNnFHfW6ldHnvckBie3OjeR?=		MlPmOkBpenN?		NWTRT5RYUW6qaXLpeIlwdiCxZjDISGFEOS9{L{OgbY4hcHWvYX6gRVI4QDCVIHPlcIx{KGG\|c3Xzd4VlKGG\|IHjpd5RwdmViSEOgZYNmfHmuYYTpc44hcW6ldXLheIVlKG[xcjC2JIhzeyCkeTDjfZRw[myxdDDhd5NigSxiRVO1NEA:KDBwMU[5OUDPxE1w	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O\|Y9N2F-
Sf9	MUPGeY5kfGmxbjDhd5NigQ>?		NHjKZWwyPSCvaX7z		NYXM[Y5rUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEPSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6\|ZXP0JHNnQSClZXzsd{B2e2mwZzDBZ{1x\XC2aXTlMWFOSyCjczDzeYJ{fHKjdHWgZZN{\XO\|ZXSgZZMhemWuZXHz[UBw\iCDTVOgdJJmcW6ldXLheIVlKG[xcjCxOUBucW6\|IH\vcIxwf2WmIHL5JJN2[nO2cnH0[UBi\GSrdHnvckBu\WG\|dYLl[EBi\nSncjCxJIhzKGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNVkxOyEQvF2u	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O\|Y9N2F-
Sf9	M3\6UWZ2dmO2aX;uJIF{e2G7		M1HPW\|E2KG2rboO=		MV\Jcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiR2PUM2MufGW{bXnuZYwhUGm\|LYTh[4dm\CCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOEApPjJ5IITvJFExQDRicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCrboPlZ5QhW2Z7IHPlcIx{KHW\|aX7nJGFkNXCncITp[IUuSU2FIHHzJJN2[nO2cnH0[UBie3Onc4Pl[EBieyC{ZXzlZZNmKG:oIFHNR{BxemWrbnP1ZoF1\WRiZn;yJFE2KG2rboOg[o9tdG:5ZXSgZpkhe3Wkc4TyZZQtKEmFNUCgQUAxNjN\|N{ig{txONg>?	NF7kN2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
Sf9	M4\seWZ2dmO2aX;uJIF{e2G7		MUKxOUBucW6\|		NGjsV3RKdmirYnn0bY9vKG:oIFOteIVzdWmwYXygTIl{NXSjZ3fl[EBpfW2jbjDy[YNwdWKrbnHueEBJTEGFOTCoOlA1KHSxIEGwOlYhemW\|aXT1[ZMqKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFHjMZBmeHSrZHWtRW1EKGG\|IIP1ZpN1emG2ZTDhd5Nme3OnZDDhd{Bz\WynYYPlJI9nKEGPQzDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XNuIFnDOVAhRSByLki4O\|gh\|ryPLh?=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O\|Y9N2F-
Sf9	NWLhTllGTnWwY4Tpc44h[XO\|YYm=		NILj[o4yPSCvaX7z		NUP4TWJxUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDy[YNwdWKrbnHueEBJTEGFMUGg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCCVZkmgZ4VtdHNidYPpcochSWNvcHXweIll\S2DTVOgZZMhe3Wkc4TyZZRmKGG\|c3Xzd4VlKGG\|IILlcIVie2Vib3[gRW1EKHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bnd2yub4fl[EBjgSC\|dXLzeJJifGViYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOSCqcjDifUBndHWxcnXzZ4Vv[2ViLDDJR\|UxKD1iND6xNVIh\|ryPLh?=	M4m5R\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9	NEXUNXNHfW6ldHnvckBie3OjeR?=		NYnYZ41kOTVibXnudy=>		MWHJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiR2PUMZRi\2enZDDoeY1idiC{ZXPvcYJqdmGwdDDISGFEPyBqNUG4JJRwKGWwZDDy[ZNq\HWnczmg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCCVZkmgZ4VtdHNidYPpcochSWNvcHXweIll\S2DTVOgZZMhe3Wkc4TyZZRmKGG\|c3Xzd4VlKGG\|IILlcIVie2Vib3[gRW1EKHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bnd2yub4fl[EBjgSC\|dXLzeJJifGViYXTkbZRqd25ibXXhd5UtKEmFNUCgQUA1NjN3NDFOwG0v	M{TPUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9	MUjGeY5kfGmxbjDhd5NigQ>?				M3rzeWlvcGmkaYTpc44hd2ZiQz30[ZJucW6jbDDIbZMufGGpZ3XkJIFv\CCFLYTldo1qdmGuIF\MRWcufGGpZ3XkJIZ2dGxibHXu[5RpKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDjc4V5eHKnc4Pl[EBqdiCoYXzsJIFzdXm5b4LtJHNnQSClZXzsd{B2e2mwZzDjZZJjd3i7Znz1c5Jme2OnaX6gLGZCVSlvbHHi[Yxm\CCjY3X0fYxifGWmLzD0dolndHWxcn;hZ4V1gWyjdHXkJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKCxiSVO1NEA:KDBwMECxJO69VS5?	NH3Rb3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O3O\|g3PCd-MkezO\|c5PjR:L3G+
Sf9	NEPGSmlHfW6ldHnvckBie3OjeR?=		NH\tRlk3OCCvaX7z		MXLJcohq[mm2aX;uJIZ2dGxibHXu[5RpKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOyJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDjc4V5eHKnc4Pl[EBqdiCoYXzsJIFzdXm5b4LtJHNnQSClZXzsd{B2e2mwZzDjZZJjd3i7Znz1c5Jme2OnaX6gLGZCVSlvbHHi[Yxm\CCjY3X0fYxifGWmLzD0dolndHWxcn;hZ4V1gWyjdHXkJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5yMEKg{txONg>?	NHPHU2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O3O\|g3PCd-MkezO\|c5PjR:L3G+
Sf9	MV;GeY5kfGmxbjDhd5NigQ>?		MmLmOlAhdWmwcx?=		MVPJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDjc4V5eHKnc4Pl[EBqdiCoYXzsJIFzdXm5b4LtJHNnQSClZXzsd{B2e2mwZzDjZZJjd3i7Znz1c5Jme2OnaX6gLGZCVSlvbHHi[Yxm\CCjY3X0fYxifGWmLzD0dolndHWxcn;hZ4V1gWyjdHXkJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5yMEKg{txONg>?	NVviW2U1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezO\|c5PjRpPkK3N\|c4QDZ2PD;hQi=>
Sf9	MYnGeY5kfGmxbjDhd5NigQ>?		NVzkS3RMPjBibXnudy=>		M3TMfmlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDHV3QufGGpZ3XkJIZ2dGxibHXu[5RpKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO1JIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDjc4V5eHKnc4Pl[EBqdiCoYXzsJIFzdXm5b4LtJHNnQSClZXzsd{B2e2mwZzDjZZJjd3i7Znz1c5Jme2OnaX6gLGZCVSlvbHHi[Yxm\CCjY3X0fYxifGWmLzD0dolndHWxcn;hZ4V1gWyjdHXkJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4Lld4NmdixiSVO1NEA:KDBwMEmyJO69VS5?	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN5N{i2OEc,Ojd\|N{e4OlQ9N2F-
Sf9	NVHlc5c5TnWwY4Tpc44h[XO\|YYm=		M4HwTFYxKG2rboO=		MXjJcohq[mm2aX;uJI9nKENvdHXycYlv[WxiSHnzMZRi\2enZDDmeYxtKGynbnf0bEBpfW2jbjDy[YNwdWKrbnHueEBJTEGFODDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNiY3;lfJBz\XO\|ZXSgbY4h\mGubDDhdo16f2:{bTDT[lkh[2WubIOgeZNqdmdiY3HyZo95gW[udX;y[ZNk\WmwIDjGRW0qNWyjYnXs[YQh[WOndInsZZRm\C9idILp[ox2d3KxYXPleJlt[XSnZDDw[ZB1cWSnIHHzJJN2[nO2cnH0[UBi\nSncjC2NEBucW6\|IHL5JIZtfW:{ZYPj[Y4tKEmFNUCgQUAxNjJ\|MTFOwG0v	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN5N{i2OEc,Ojd\|N{e4OlQ9N2F-
Sf9	MmH6SpVv[3Srb36gZZN{[Xl?				M1HtPGlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDHV3QufGGpZ3XkJIFv\CCFLYTldo1qdmGuIFjpd{11[WepZXSgbJVu[W5icnXjc41jcW6jboSgTGRCSzRiKE[yO{B1dyBzMEi0JJJme2mmdXXzJEkh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJINw\XiycnXzd4VlKGmwIH\hcIwh[XKveYfvdo0hW2Z7IHPlcIx{KHW\|aX7nJINiemKxeInmcJVwemW\|Y3XpckApTkGPKT3sZYJmdGWmIHHj[ZR6dGG2ZXSvJJRzcW[udX;yc4Fk\XS7bHH0[YQheGWydHnk[UBieyxiSVO1NEA:KDBwM{ezJO69VS5?	M{nDVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5M{e3PFY1Lz5{N{O3O\|g3PDxxYU6=
Sf9	NUPYWmpmTnWwY4Tpc44h[XO\|YYm=		MmL3OlAhdWmwcx?=		MoPlTY5pcWKrdHnvckBw\iCFLYTldo1qdmGuIFjpd{11[WepZXSgbJVu[W5icnXjc41jcW6jboSgTGRCSzliKE[wOEB1dyBzME[2JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgZ49mgHC{ZYPz[YQhcW5iZnHscEBiem27d3;ycUBU\jliY3XscJMhfXOrbnegZ4Fz[m:6eX\seY9z\XOlZXnuJEhHSU1rLXzhZoVt\WRiYXPleJlt[XSnZD:geJJq\my3b4LvZYNmfHmuYYTl[EBx\XC2aXTlJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6NCCLQ{WwJF0hOi54ODFOwG0v	NEXabo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O3O\|g3PCd-MkezO\|c5PjR:L3G+
Sf9	M{fUdGZ2dmO2aX;uJIF{e2G7		Ml\pOlAhdWmwcx?=		NWrSW3g2UW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQhUESDQ{egLFUyQCC2bzDlcoQhemW\|aXT1[ZMqKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bkd2W6cILld5Nm\CCrbjDmZYxtKGG{bYn3c5JuKFOoOTDj[YxteyC3c3nu[{Bk[XKkb4j5[ox2d3Knc3PlbY4hME[DTTmtcIFj\WynZDDhZ4V1gWyjdHXkM{B1emmobIXvdo9i[2W2eXzheIVlKHCncITp[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDZyIH3pcpMh[nliLDDJR\|UxKD1iMj64N{DPxE1w	M{H0Z\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5M{e3PFY1Lz5{N{O3O\|g3PDxxYU6=
HEK293	M{XIOWN6fG:2b4jpZ4l1gSCjc4PhfS=>		Mk\qOFghcHK\|		MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KHKnc3H6eZJqdiCjc4PhfUwhUUN3MDC9JFAvODdizszNMi=>	NWS0N\|hzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyOFEyOTJpPkK4NlQyOTF{PD;hQi=>
NFF	M3\vZmN6fG:2b4jpZ4l1gSCjc4PhfS=>		NGL5OHc4OiCqcoO=		MkOyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUmZHKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO\|YYmsJGlEPTBiPTCwMlA4KM7:TT6=	M2\sdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkSxNVEzLz5{OEK0NVEyOjxxYU6=
HUT78	MW\BdI9xfG:\|aYOgZZN{[Xl?		MV[xPEBpenN?		MX3Qdo8u[XCxcITveIlkKGGldHn2bZR6KGmwIHj1cYFvKEiXVEe4JINmdGy\|IHHmeIVzKDF6IHjyd{BjgSClYYPwZZNmNUeubzCzM\|ch[XO\|YYmsJGVEPTBiPTCwMlAxPDNizszNMi=>	Mkj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzMkKyNlcoRjNyMUKyNlI4RC:jPh?=
NFF	NFvnbnVEgXSxdH;4bYNqfHliYYPzZZk>		NIO0dno4OiCqcoO=		M3vNe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG5HTiClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NEA:KDBwMEeg{txONg>?	M2n3fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkS1OFAzLz5\|MEK0OVQxOjxxYU6=
HEK293	NHnXdWFEgXSxdH;4bYNqfHliYYPzZZk>		MnjTOFghcHK\|		MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KHKnc3H6eZJqdiCmeXWgZoF{\WRiYYPzZZktKEmFNUCgQUAxNjB5IN88UU4>	MnTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NEW0NFIoRjNyMkS1OFAzRC:jPh?=
M14	NH\tNWtCeG:ydH;zbZMh[XO\|YYm=		NY[1TY12OjRidH:gOFghcHK\|		MULJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGi3bXHuJG0yPCClZXzsd{Bie3Onc4Pl[EBieyClYYPwZZNmKGGldHn2bZR6KGGodHXyJFI1KHSxIES4JIhzeyC3c3nu[{BFTV[GIIDldJRq\GViYYOgd5Vje3S{YYTlJIJ6KEGyb2TvfE1IdG9idILpdIxmgCCjc4PhfS=>	NGL5VZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3NVQ3Pid-MkS0O\|E1PjZ:L3G+
U937	NU\tPXhPTnWwY4Tpc44h[XO\|YYm=	MUCxJJVO	NXGycmgyOjRiaILz		MXHJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJHU6OzdiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2Vib3[gbY51emGlZXzseYxieiCjY3X0fYxifGWmIHHsdIhiNXS3YoXsbY4hdGW4ZXygZZQhOSC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	M4TQ[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nkm0NFU2Lz5{NE[5OFA2PTxxYU6=
U937	M2XqT2Z2dmO2aX;uJIF{e2G7	MUCxJJVO	MV[yOEBpenN?		M{HGPGlvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hXTl\|NzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDv[kBqdnS{YXPlcIx2dGG{IHHj[ZR6dGG2ZXSgbIl{fG:wZTDIN{Bt\X[nbDDheEAyKHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	NVvJd2dVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2PVQxPTVpPkK0Olk1ODV3PD;hQi=>
U937	NXjIZ3F1TnWwY4Tpc44h[XO\|YYm=	MnLtNUB2VQ>?	NGjOSpMzPCCqcoO=		Ml7RTY5pcWKrdHnvckBw\iCKRFHDNkBqdiCqdX3hckBWQTN5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIH;mJIlvfHKjY3XscJVt[XJiYXPleJlt[XSnZDDobZN1d26nIFizJIxmfmWuIHH0JFEhfU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7NEC1OUc,OjR4OUSwOVU9N2F-
U937	MXnGeY5kfGmxbjDhd5NigQ>?	MknCNUB2VQ>?	NX7LU\|k{OjRiaILz		MUHJcohq[mm2aX;uJI9nKEiGQVOzJIlvKGi3bXHuJHU6OzdiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2Vib3[gbY51emGlZXzseYxieiCjY3X0fYxifGWmIHjpd5RwdmViSESgcIV3\WxiYYSgNUB2VSCjZoTldkAzPCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|	M4K4UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nkm0NFU2Lz5{NE[5OFA2PTxxYU6=
MV4-11	NILpUHZHfW6ldHnvckBie3OjeR?=	NFrtbpUyOCC2bzCxNFAxKG6P	NWDje\|lpPiCqcoO=		M2XmWWlvcGmkaYTpc44hd2ZiSFTBR\|YhcW5iaIXtZY4hVVZ2LUGxJINmdGy\|IHHzd4V{e2WmIHHzJJVxemWpdXzheIlwdiCxZjDhcJBp[SC2dXL1cIlvKGGlZYT5cIF1cW:wIHH0JFExKHSxIEGwNFAhdk1iYX\0[ZIhPiCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR2M{C3PEc,OjZ2NEOwO\|g9N2F-
HCT116	M4DzeWZ2dmO2aX;uJIF{e2G7	MkX0NVAhfG9iMUCwNEBvVQ>?	MYi2JIhzew>?		NFvGWVlKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{B2eHKnZ4XsZZRqd25ib3[gZYxxcGFidIXieYxqdiCjY3X0fYxifGmxbjDheEAyOCC2bzCxNFAxKG6PIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	MlvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2NEOwO\|goRjJ4NESzNFc5RC:jPh?=
HCT116	MlzESpVv[3Srb36gZZN{[Xl?	Mn3qNVAhfG9iMUCwNEBvVQ>?	NUjqUXlbPiCqcoO=		NET3fXpKdmirYnn0bY9vKG:oIFjERWMyNzJxMzDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XO\|ZYPz[YQh[XNidYDy[Yd2dGG2aX;uJI9nKGirc4TvcoUhUDNiYXPleJlt[XSrb36gZZQhOTBidH:gNVAxOCCwTTDh[pRmeiB4IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR2M{C3PEc,OjZ2NEOwO\|g9N2F-
MV4-11	M374bmZ2dmO2aX;uJIF{e2G7	MVWxNEB1dyBzMECwJI5O	MV[2JIhzew>?		M3zDWGlvcGmkaYTpc44hd2ZiSFTBR\|EwOi9\|IHnuJIh2dWGwIF3WOE0yOSClZXzsd{Bie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4YhcGm\|dH;u[UBJOyCjY3X0fYxifGmxbjDheEAyOCC2bzCxNFAxKG6PIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	NYXlbIhDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0OFMxPzhpPkK2OFQ{ODd6PD;hQi=>
Raji	MnvkR5l1d3SxeHnjbZR6KGG\|c3H5		M2rCUlI1KGi{cx?=		MkD3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6	MnLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O\|YoRjJ5MUi2Olc3RC:jPh?=
Ramos	NX;2R5lUS3m2b4TvfIlkcXS7IHHzd4F6		NWDmdGg6OjRiaILz		MnrWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVoFud3NiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>?	NGDCd4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
U266	NFvqZoFEgXSxdH;4bYNqfHliYYPzZZk>		M1LYTVI1KGi{cx?=		M1L2[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHUzPjZiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>?	M{nhXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
RPMI8226	MljzR5l1d3SxeHnjbZR6KGG\|c3H5		Ml:3NlQhcHK\|		NFH3UXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTWE2LOEKyOkBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5	M3WwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
HBL1	M1vSfWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NWnZfohGOjRiaILz		NXrCUnJES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEKOMTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7	MnXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O\|YoRjJ5MUi2Olc3RC:jPh?=
MM1S	M1fOZmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MoXINlQhcHK\|		NXrwUYxxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVU1zUzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7	NYTWblN[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPFY3PzZpPkK3NVg3Pjd4PD;hQi=>
OCI-LY1	NXW2ZZJoS3m2b4TvfIlkcXS7IHHzd4F6		MX[yOEBpenN?		MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDPR2kuVFlzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[Xl?	NH\YRZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
SUDHL4	M{Tjd2N6fG:2b4jpZ4l1gSCjc4PhfS=>		NYHlbZVMOjRiaILz		M3jRN2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNWTEiONDDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7	NWDQW3FRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPFY3PzZpPkK3NVg3Pjd4PD;hQi=>
MV4-11	M4XOeGZ2dmO2aX;uJIF{e2G7	M3PlNVUxKG6P	MX:yOEBpenN?		NWXpWYxYUW6mdXP0bY9vKG:oIFjERWM3KGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3WOE0yOSClZXzsd{BifCB3MDDuUUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	M3rGbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
MV4-11	NYjtcFBVTnWwY4Tpc44h[XO\|YYm=	NELs[HU2OCCwTR?=	MVGyJJRwKDJ2IHjydy=>		MUHJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJG1XPC1zMTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WyyaHGteJVjfWyrbjDofZBmemGlZYT5cIF1cW:wIHH0JFUxKG6PIHHmeIVzKDJidH:gNlQhcHK\|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q>	NFy3SVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
MV4-11	Mor0R4VtdCCleXPs[UBienKnc4SgZZN{[Xl?	NGH0fHc{OCC2bzC1NEBvVQ>?	MlznNlQhcHK\|		Mk[yR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHHjZ5VufWyjdHnvckBifCC\|dXKtS\|EheGijc3WgZZQhOzBidH:gOVAhdk1iYX\0[ZIhOjRiaILzJIJ6KHC{b4Dp[Il2dSCrb3Tp[IUhe3SjaX7pcocu[mG\|ZXSg[oxwfyCleYTvcYV1emmlIH3leIhw\A>?	NXfaUVNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
MV4-11	NWXKNYJFSXCxcITvd4l{KGG\|c3H5	Mm\iN\|Ahdk1?	NVi5d2Z{OjRidH:gOFghcHK\|		MWTJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2YND2xNUBk\WyuczDheEA{OCCwTTDh[pRmeiB{NDD0c{A1QCCqcoOgZpkhSW6wZYjpckBXNVCLIIP0ZYlvcW6pIHLhd4VlKG[ub4egZ5l1d22ndIL5	Ml7CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5M{i5OVMoRjJ7N{O4PVU{RC:jPh?=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	DNMT1 / EZH2 ; caspase-8 / cleaved caspase-8 / Sp1 ; c-Myc / IRF4 ; Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1 ; RAD51 / BRCA1 / CHK1 / RPL13a ; H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP	19279403 27738323 28915627 24244429 31071955
Immunofluorescence	Synaptophysin / NACM ; α-tubulin / Acetyl-α-tubulin ; BiP ; ATF4 ; IRE1α / S724-IRE1α	28915627 29983882 23544167
Growth inhibition assay	Cell viability	27738323

In vivo

In lung cancer and mesothelioma animal models, LBH589 markedly decreases tumor growth by 62%. LBH589 is equally effective in immunocompetent and severe combined immunodeficien-cymice, suggesting that the inhibition of tumor growth by LBH589 is not due to direct immunologic effects. Daily LBH589, given i.p. at 20 mg/kg for 5 days per week, leading to an average decrease in growth of 70%. Compared with the corresponding control tumors, LBH589 leads to a 53% decrease for H526-derived tumors, an 81% decrease for BK-T-derived tumors, a 76% decrease for RG-1- derived tumors, and a 70% decrease for H69-derived tumors. In contrast to the lack of tumor regression notes in NSCLC and Meso-derived xenografted tumors that are treated under identical conditions and doses, LBH589 results in dramatic tumor regression in SCLC-derived tumors and RG-1-derived tumor. ^[2]

Protocol (from reference)

Cell Research:^[1]

Cell lines: MOLT-4 cell lines and Reh (pre-B cells)
Concentrations: 50 nM
Incubation Time: 48 hours
Method: Untreated and LBH589-treated cells [human Ph- acute lymphoblastic leukemia MOLT-4 (T cells) and Reh (pre-B cells)] are stained with annexin V and propidium iodide using annexin V-FITC apoptosis detection kit I. The percentage of apoptotic and nonviable cells is determined by flow cytometry. At least 5 × 10⁴ cells are collected with a CyAn ADP Violet cytometer. Percentage apoptosis is calculated considering all the annexin V-positive plus the annexin V/PI-positive cells; percentage loss of cell viability is calculated considering all the annexin V-positive plus the PI-positive and the annexinV/PI-positive cells.
(Only for Reference)

Animal Research:^[2]

Animal Models: Severe combined immunodeficiency (SCID) mice with M30 (10⁷ cells) or A549 (5 × 10⁶ cells), H69 (2.5 × 10⁶ cells), BK-T (6.5 × 10⁶), H526 (10 × 10⁶), and RG1 (10 × 10⁶) cells
Dosages: 10 mg/kg, 20 mg/kg
Administration: Administered via i.p. injection
(Only for Reference)

References

+ Expand

Solubility (25°C)

In vitro	DMSO	69 mg/mL (197.46 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 2% DMSO+48% PEG 300+2% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	349.43
Formula	C₂₁H₂₃N₃O₂
CAS No.	404950-80-7
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1=C(C2=CC=CC=C2N1)CCNCC3=CC=C(C=C3)C=CC(=O)NO

Download Panobinostat (LBH589) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04341311	Active not recruiting	Drug: Marizomib\|Drug: Panobinostat	Diffuse Intrinsic Pontine Glioma\|Pediatric Brainstem Glioma\|Pediatric Brainstem Gliosarcoma Recurrent\|Pediatric Cancer\|Pediatric Brain Tumor\|Diffuse Glioma	Dana-Farber Cancer Institute\|Celgene\|Secura Bio Inc.	August 10 2020	Phase 1
NCT03632317	Withdrawn	Drug: Panobinostat\|Drug: Everolimus	Glioma\|Diffuse Intrinsic Pontine Glioma	University of Michigan Rogel Cancer Center	October 2019	Phase 2
NCT03982134	Withdrawn	Drug: PDR001\|Drug: Panobinostat	Melanoma\|Non Small Cell Lung Cancer	Muhammad Furqan\|Novartis Pharmaceuticals\|University of Iowa	September 2019	Phase 1
NCT04326764	Recruiting	Drug: Panobinostat	Acute Myeloid Leukaemia (AML)\|Myelodysplastic Syndromes (MDS)	Goethe University\|Jan J. Cornelissen (HOVON-SAKK)\|Sebastian Giebel (PALG)	July 24 2018	Phase 3
NCT03515915	Unknown status	--	Patients With Recurrent or Refractory Multiple Myeloma	University Hospital Montpellier\|Poitiers University Hospital	April 23 2018	--

(data from https://clinicaltrials.gov, updated on 2021-09-06)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo mice study?

Answer:
We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):