Panobinostat (LBH589)

Catalog No.S1030 Synonyms: NVP-LBH589

For research use only.

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.

Panobinostat (LBH589) Chemical Structure

CAS No. 404950-80-7

Selleck's Panobinostat (LBH589) has been cited by 325 publications

Purity & Quality Control

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Biological Activity

Description Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Targets
HDAC (MOLT-4 cells) [1] HDAC (Reh cells) [1]
5 nM 20 nM
In vitro

LBH589 induces apoptosis among MOLT-4 and Reh cells in a time- and dose-dependent manner. Moreover, LBH589 is more potent in MOLT-4 than in Reh cells. LBH589 markedly prevents the growth of both MOLT-4 and Reh cells in a dose-dependent manner at 48 hours. LBH589 treatment causes a 2- to 3-fold increase in the number of cells in the G2/M phase of the cell cycle compared with the control cells. LBH589 is associated with induction of histone H3K9 and histone H4K8 acetylation as well as decreasing levels of c-Myc expression in a dose-dependent manner. LBH589 treatment also increases the levels of p21 expression. LBH589 treatment also decreases the levels of c-Myc after an initial increase at the lowest dose (10 nM) in Reh cells. In addition, LBH589 gives rise to substantial increases in mRNA levels of proapoptosis and DNA repair genes. LBH589 induces increased levels of acetylated histone H3 and H4 at the GADD45G promoter. [1] Besides, LBH589 inhibits growth of non small cell lung cancer cell lines (such as human H1299, L55 and A549 with IC50 of 5 nM, 11 nM and 30 nM, respectively), mesothelioma (such as human OK-6 and Ok-5 with IC50 of 5 nM and 7 nM, respectively) and small cell lung cancer cell lines (such as human RG-1 and LD-T with IC50 of 4 nM and 5 nM, respectively). [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT29 M{\2Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zWWlAuOTBizszN NVrnR2dIOC12IHS= NIPCWGFqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M1\KWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{CyO|g1Lz5{NkewNlc5PDxxYU6=
HepG2 MmqzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmO1NE0yOCEQvF2= M1Xac|AuPCCm NWTPc|dNcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NVvvNph2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFI4QDRpPkK2O|AzPzh2PD;hQi=>
HT29 NEnY[5RHfW6ldHnvckBCe3OjeR?= M4TGTVUxyqCwTR?= M17UOVI1NTd{IHi= Mmq3bY5lfWOnZDDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UhOyCjZoTldkA1QMLiaNMg NYjsRmFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFI4QDRpPkK2O|AzPzh2PD;hQi=>
HepG2 MUDGeY5kfGmxbjDBd5NigQ>? M4XPdlUxyqCwTR?= MYeyOE04OiCq NETWenpqdmS3Y3XkJIFkfGm4YYTpc44hd2ZiY3HzdIF{\SB|IHHmeIVzKDJ2wrDoxsA> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HCC827 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\xcphkPS95LkWvNVAhdk1? MlTtO|LDqGh? MmXRSG1UVw>? Ml\G[Y5p[W6lZYOgeIhmKGGwdHnwdo9tcW[ncnH0bZZmKGWoZnXjeEBw\iCncnzveIlvcWJ? MkXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4N{W0PFQoRjJ4Nke1OFg1RC:jPh?=
A549  MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzvWZUyOC9zNT:yNEBvVQ>? M2jaNFczyqCq NVrnN5FOTE2VTx?= MXflcohidmOnczD0bIUh[W62aYDyc4xq\mW{YYTpeoUh\W[oZXP0JI9nKGW{bH;0bY5q[g>? NVzVbWVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2O|U1QDRpPkK2Olc2PDh2PD;hQi=>
NCI-H460  MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfMe4UyOC9{MD:zNEBvVQ>? NGm1XpE4OsLiaB?= MnzMSG1UVw>? NITCeYFmdmijbnPld{B1cGViYX70bZBzd2yrZnXyZZRqfmViZX\m[YN1KG:oIHXycI91cW6rYh?= M{\yRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nke1OFg1Lz5{Nk[3OVQ5PDxxYU6=
J89GFP MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvSSG1UV8Li MmHKSWM2OD12OT64OUDDuSBzMj62OUBvVQ>? NHPaPFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2N|U3QCd-Mk[1OlM2Pjh:L3G+
THP89GFP NHq1bGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TQZ2ROW00EoB?= MWrFR|UxRTF7LkO0JOKyKDZwNEOgcm0> NVrDfolSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlM2PjhpPkK2OVY{PTZ6PD;hQi=>
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G401 M2DzTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLRTXFGOC5yMfMAl|ExNjBizszN NHfn[4UzPCCq M4XC[2ROW00EoB?= Mmr0TWM2OD1zNEOuNFIhdk1? NGPJdHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
SK-NEP-1 M{jwcWNmdGxiVnnhZoltcXS7IFHzd4F6 MYW1NEBvVQ>? MYGx5qCUPCCm Mnn4SG1UV8Li NYLERmJDemWmdXPld{Bk\WyuIIP1dpZqfmGuIHnuJIEhfGmvZTDk[ZBmdmSnboSgcYFvdmW{ MnfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
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SK-NEP-1 M1q3fmFxd3C2b4Ppd{BCe3OjeR?= MkHFOVAwOTByIH7N MnrBNlQhcA>? NFzZbI5FVVORwrC= Ml:5bY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 MmTzRZBweHSxc3nzJGF{e2G7 M{LkS|UxNzFyMDDuUS=> NF;5T5UzPCCq MVXEUXNQyqB? M4PMd4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MmnIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
SK-NEP-1 MlT5SpVv[3Srb36gRZN{[Xl? M{DDfFUxNzFyMDDuUS=> NWDiNY1xOjRiaB?= M2jue2ROW00EoB?= NX\Lc3REe2ixd4OgeIhmKGmwZIXjeIlwdiCxZjDEUmEh\nKjZ33lcpRifGmxbh?= NVf3bJpKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 NHK2W5dHfW6ldHnvckBCe3OjeR?= M{i5TlUxNzFyMDDuUS=> MnjhNlQhcA>? M1fhNmROW00EoB?= MXLzbI94eyC2aHWgbY5lfWO2aX;uJI9nKESQQTDmdoFodWWwdHH0bY9v MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
SK-NEP-1 NWDCbGRbTnWwY4Tpc44hSXO|YYm= NGrMW442OC9zMECgcm0> M2jR[VI1KGh? NVfYW2h2TE2VT9Mg NYK0NmRHcW6mdXPld{Bk\WyuIHP5Z4xmKGSrc3;y[IVzyqB? NXnoWJJwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 M4LIdGZ2dmO2aX;uJGF{e2G7 MX[1NE8yODBibl2= MXyyOEBp M4jES2ROW00EoB?= MlHFbY5lfWOnczDj[YxtKGO7Y3zlJIRqe2:{ZHXyxsA> M1;iXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
RPMI 8226 MWLD[YxtKFO3co\peoFtKEG|c3H5 MoXhNk81NzZibl2= MoX5OFjjiImq MmHwbY5lfWOnczDhJJNq\26rZnnjZY51KGSnY4LlZZNmKGmwIITo[UBk\WyuIHfyc5d1cA>? Mnv0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
OPM2 NXG5bmFlS2WubDDTeZJ3cX[jbDDBd5NigQ>? MkPjNk81NzZibl2= MVW0PQKBkWh? M4HRUYlv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? NY\V[ZI6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
U266 M3nLWmNmdGxiU4Xyeol3[WxiQYPzZZk> MX[yM|QwPiCwTR?= MXO0PQKBkWh? M1nyO4lv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
H929 M{jwV2NmdGxiU4Xyeol3[WxiQYPzZZk> MX2yM|QwPiCwTR?= MXq0PQKBkWh? NUXZ[pRlcW6mdXPld{BiKHOrZ37p[olk[W62IHTlZ5Jm[XOnIHnuJJRp\SClZXzsJIdzd3e2aB?= M4jqVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwNlkzLz5{NkCwNFI6OjxxYU6=
RPMI 8226  M3:zTGFxd3C2b4Ppd{BCe3OjeR?= M2e4blTjiImwTR?= Ml7ONlQwPDhiaB?= MoL2bY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? NGnhbJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCwNFI6Oid-Mk[wNFAzQTJ:L3G+
HCC827 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33EZVExKG6P M1O2VFQ5KGh? NWnZc4t6TE2VTx?= NFiyVopmdmijbnPld{BkcXOybHH0bY4he2Wwc3n0bZZqfHoEoB?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl2NE[xO{c,OjV7NES2NVc9N2F-
NCI-H23 NUfMfZJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWCxNEBvVQ>? M3XSPVQ5KGh? MV\EUXNQ NHTxd3RmdmijbnPld{BkcXOybHH0bY4he2Wwc3n0bZZqfHoEoB?= M4n1N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUS0OlE4Lz5{NUm0OFYyPzxxYU6=
AML3 M1nvdWZ2dmO2aX;uJGF{e2G7 MlLINE0yKM7:TR?= M4HDVVI1yqCq MnnwbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NYLUS3l2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
ML-1 NIe3PFBHfW6ldHnvckBCe3OjeR?= MUOwMVEh|ryP MXSyOOKhcA>? MoDrbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NWf4WZV2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
RPMI-8226vr10  M2L6ZWZ2dmO2aX;uJGF{e2G7 MYWwMVEh|ryP M4SwN|I1yqCq NYfMTmtDcW6mdXPld{BFVkFiZoLh[41mdnSjdHnvcuKhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NFK2NIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[xNlk1OSd-MkW2NVI6PDF:L3G+
ML-1 Mm\zSpVv[3Srb36gRZN{[Xl? Mn3WNUDPxE1? MV[yOOKhcA>? Mo\VbY5kemWjc3XzJINie3Cjc3WtN{Bi[3Srdnn0fUA1NW[xbHS= NEHmOVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[xNlk1OSd-MkW2NVI6PDF:L3G+
RPMI-8226vr10  M{D4TmZ2dmO2aX;uJGF{e2G7 NVPWWHB5OSEQvF2= M3;QNFI1yqCq M{jGSIlv[3KnYYPld{Bk[XOyYYPlMVMh[WO2aY\peJkhOi53LX\vcIQ> M1\nPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
SK-N-BE (2) MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fHN|I16oDLaB?= NGXidIhKSzVyPUGwOE4x6oDLwsJihKk4Njhibl2= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV|MEi5NVY9N2F-
SK-N-BE (2), PAN  MK MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnuWWgzPOLCiXi= Ml7rTWM2OD1zMESuNQKBkcLz4pEJO{45KG6P NWDvXHhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N|A5QTF4PD;hQi=>
SK-N-BE (2), MK  PAN MnvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITM[5IzPOLCiXi= NYe0WYJyUUN3ME2zPFIvOOLCidMx5qCKPDNwMjDuUS=> MmXkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-AS MnzCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4mwU|I16oDLaB?= NY\6S41yUUN3ME2zO{4y6oDLwsJihKkzNjRibl2= MlrPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-DZ NWLLeVRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzOdpdzOjUkgJno NF\adXlKSzVyPUG3MlHjiIoEsfMAjVAvPCCwTR?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV|MEi5NVY9N2F-
Caki-1 MoXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHISYdpOTBxMkWvOVAhdk1? M{XqelQ5KGh? M3zpUolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVzKHO7bnXy[4l{fGmlYXzsfUB4cXSqIILpeI9v[X[rch?= M3K3fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke5NVkyLz5{NUK3PVE6OTxxYU6=
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SK-N-BE MXvGeY5kfGmxbjDBd5NigQ>? NUf0RYo6OOLCk{SwJI5O Mon5OFghcA>? NX6yfWZvcW6mdXPld{BiKGSxc3Wt[IVx\W6mZX70JINt\WG4YXflJI9nKGOjc4Dhd4UhOyCjbnSgVGFTWA>? MmjJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
HCC-LM3 M4n5TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnPW|EyNTFyMECgcm0> MlfmNlQwPDhxN{KgbC=> NHfje5RFVVOR MYPpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NY\vbZJHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
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HepG2 M3K2ZmFxd3C2b4Ppd{BCe3OjeR?= Mmr1OVAhdk1? NHXhPXY1QCCq NGDueHBFVVOR M2DuR4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3nncolncWOjboTsfUBqdiCjIHPhd5Bie2VvZHXw[Y5l\W62IH3hco5meiCkeTDjcIVifmGpZTDv[kBk[XOyYYPld{A{NCB6IHHu[EA6 MkGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 MoPmRZBweHSxc3nzJGF{e2G7 MWS1NEBvVQ>? M1HLVlQ5KGh? NEOxRZZFVVOR MmjGbY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|aXfubYZq[2GwdHz5JIlvKGFiY3HzdIF{\S2mZYDlcoRmdnRibXHucoVzKGK7IHPs[YF3[WenIH;mJINie3Cjc3XzJFMtKDhiYX7kJFk> MlixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HCC-LM3 NYe1ZWs3TnWwY4Tpc44hSXO|YYm= NYLLNGNyPTBxMUCwJI5O M3;FeVI1KGh? NWq1UHJITE2VTx?= M3zGfYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC= NHHI[lc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HepG2 M2O1cWZ2dmO2aX;uJGF{e2G7 NWftWXBVPTBxMUCwJI5O MYCyOEBp MoLESG1UVw>? NXXTNlFL\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdE1UXEGWMzDhcoQheC2Da4VCpC=> NXXJOnlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
SMMC-7721 MoTQSpVv[3Srb36gRZN{[Xl? NFT0NGg2OC9zMECgcm0> NIrj[mYzPCCq NX3BZXdRTE2VTx?= NH[yXIRl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDwMXNVSVR|IHHu[EBxNUGtdNMg Mmq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HCC-LM3 NHGySWJHfW6ldHnvckBCe3OjeR?= MlHCOVAwOTByIH7N NUO4cYgyOjRiaB?= NUnDPG9oTE2VTx?= M3XHZ4Rwf26{ZXf1cIF1\XNiQnPsMZhNKGW6cILld5Nqd25? NH\2NFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HepG2 MoDLSpVv[3Srb36gRZN{[Xl? MYq1NE8yODBibl2= M{XId|I1KGh? MlLySG1UVw>? MofQ[I94dnKnZ4XsZZRmeyCEY3ytfGwh\XiycnXzd4lwdg>? M2rXWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
SMMC-7721 MmjjSpVv[3Srb36gRZN{[Xl? NXHRcnBrPTBxMUCwJI5O M3q1NFI1KGh? MofrSG1UVw>? MmHq[I94dnKnZ4XsZZRmeyCEY3ytfGwh\XiycnXzd4lwdg>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
FaDu NGGyW3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HzTVExOOLChX7N NFzCc2Q5NzFyL{GyJIg> NHHNUodlcXOybHH5[YQh[SC|aXfubYZq[2GwdDDhcoQheHKxbH;u[4VlKEd{L12gZZJz\XO2IHH0JFgh[W6mIEGy5qCGcCCyb4P0JJJmdGWjc3W= NEW1O5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyOlQ5Oid-MkSwNlY1QDJ:L3G+
FaDu NXLNbZltTnWwY4Tpc44hSXO|YYm= M2XOfVExOOLChX7N Mm[5Nk81NzhxMUKgbC=> MljNbY5lfWOnZDDwNlFY[WZzL1PpdFHDqGW6cILld5Nqd25? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB{NkS4Nkc,OjRyMk[0PFI9N2F-
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RWPE-1  M1zuUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCwMVIxKM7:TR?= NXfZSIVSOjRxNEivO|IhcA>? MXjpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NFLwV|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m5NVIyPid-MkO5PVEzOTZ:L3G+
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CFPAC-1  M2rNRWZ2dmO2aX;uJGF{e2G7 MW[yOU82OC9zMECgcm0> M2DPV|gwOjRxNEigbC=> M3r0bmROW09? M4K5UYRwf26{ZXf1cIF1\WRiUn;uJI1TVkFiYX7kJJBzd3SnaX6g[ZhxemW|c3nvckBidmRiZH;3cpN1emWjbTDzbYdv[Wyrbne= NX\XVHg5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
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CFPAC-1  NGfnfnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzycYEzPS93MD:xNFAhdk1? MUm0PEBp M17SeGROW09? NIDKbZhz\WS3Y3XzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl{Mki4Okc,OjN7MkK4PFY9N2F-
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MV4-11 NGLIW5ZHfW6ldHnvckBie3OjeR?= NYfFUXhHPTBibl2= NIjuOJIzKHSxIEK0JIhzew>? M1TORmlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hVVZ2LUGxJINmdGy|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iCjbIDoZU11fWK3bHnuJIh6eGW{YXPleJlt[XSrb36gZZQhPTBibl2gZYZ1\XJiMjD0c{AzPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? M3T2[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
MV4-11 NHfXO5FE\WyuIHP5Z4xmKGG{cnXzeEBie3OjeR?= MUSzNEB1dyB3MDDuUS=> M4j3PVI1KGi{cx?= Ml\CR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCC|dXKtS|EheGijc3WgZZQhOzBidH:gOVAhdk1iYX\0[ZIhOjRiaILzJIJ6KHC{b4Dp[Il2dSCrb3Tp[IUhe3SjaX7pcocu[mG|ZXSg[oxwfyCleYTvcYV1emmlIH3leIhw\A>? NVPDdoM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
MV4-11 M3\XVmFxd3C2b4Ppd{Bie3OjeR?= NVvL[|c2OzBibl2= MUmyOEB1dyB2ODDodpM> Mn3ETY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCPVkStNVEh[2WubIOgZZQhOzBibl2gZYZ1\XJiMkSgeI8hPDhiaILzJIJ6KEGwbnX4bY4hXi2SSTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd|OEm1N{c,Ojl5M{i5OVM9N2F-
Assay
Methods Test Index PMID
Western blot DNMT1 / EZH2 ; caspase-8 / cleaved caspase-8 / Sp1 ; c-Myc / IRF4 ; Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1 ; RAD51 / BRCA1 / CHK1 / RPL13a ; H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP 19279403 27738323 28915627 24244429 31071955
Immunofluorescence Synaptophysin / NACM ; α-tubulin / Acetyl-α-tubulin ; BiP ; ATF4 ; IRE1α / S724-IRE1α 28915627 29983882 23544167
Growth inhibition assay Cell viability 27738323
In vivo In lung cancer and mesothelioma animal models, LBH589 markedly decreases tumor growth by 62%. LBH589 is equally effective in immunocompetent and severe combined immunodeficien-cymice, suggesting that the inhibition of tumor growth by LBH589 is not due to direct immunologic effects. Daily LBH589, given i.p. at 20 mg/kg for 5 days per week, leading to an average decrease in growth of 70%. Compared with the corresponding control tumors, LBH589 leads to a 53% decrease for H526-derived tumors, an 81% decrease for BK-T-derived tumors, a 76% decrease for RG-1- derived tumors, and a 70% decrease for H69-derived tumors. In contrast to the lack of tumor regression notes in NSCLC and Meso-derived xenografted tumors that are treated under identical conditions and doses, LBH589 results in dramatic tumor regression in SCLC-derived tumors and RG-1-derived tumor. [2]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: MOLT-4 cell lines and Reh (pre-B cells)
  • Concentrations: 50 nM
  • Incubation Time: 48 hours
  • Method: Untreated and LBH589-treated cells [human Ph- acute lymphoblastic leukemia MOLT-4 (T cells) and Reh (pre-B cells)] are stained with annexin V and propidium iodide using annexin V-FITC apoptosis detection kit I. The percentage of apoptotic and nonviable cells is determined by flow cytometry. At least 5 × 104 cells are collected with a CyAn ADP Violet cytometer. Percentage apoptosis is calculated considering all the annexin V-positive plus the annexin V/PI-positive cells; percentage loss of cell viability is calculated considering all the annexin V-positive plus the PI-positive and the annexinV/PI-positive cells.
Animal Research:[2]
  • Animal Models: Severe combined immunodeficiency (SCID) mice with M30 (107 cells) or A549 (5 × 106 cells), H69 (2.5 × 106 cells), BK-T (6.5 × 106), H526 (10 × 106), and RG1 (10 × 106) cells
  • Dosages: 10 mg/kg, 20 mg/kg
  • Administration: Administered via i.p. injection

Solubility (25°C)

In vitro

DMSO 69 mg/mL
(197.46 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+48% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 349.43
Formula

C21H23N3O2

CAS No. 404950-80-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C2=CC=CC=C2N1)CCNCC3=CC=C(C=C3)C=CC(=O)NO

In vivo Formulation Calculator (Clear solution)

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% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04341311 Active not recruiting Drug: Marizomib|Drug: Panobinostat Diffuse Intrinsic Pontine Glioma|Pediatric Brainstem Glioma|Pediatric Brainstem Gliosarcoma Recurrent|Pediatric Cancer|Pediatric Brain Tumor|Diffuse Glioma Dana-Farber Cancer Institute|Celgene|Secura Bio Inc. August 10 2020 Phase 1
NCT03632317 Withdrawn Drug: Panobinostat|Drug: Everolimus Glioma|Diffuse Intrinsic Pontine Glioma University of Michigan Rogel Cancer Center October 2019 Phase 2
NCT03982134 Withdrawn Drug: PDR001|Drug: Panobinostat Melanoma|Non Small Cell Lung Cancer Muhammad Furqan|Novartis Pharmaceuticals|University of Iowa September 2019 Phase 1
NCT04326764 Active not recruiting Drug: Panobinostat Acute Myeloid Leukaemia (AML)|Myelodysplastic Syndromes (MDS) Goethe University|Jan J. Cornelissen (HOVON-SAKK)|Sebastian Giebel (PALG) July 24 2018 Phase 3
NCT03515915 Unknown status -- Patients With Recurrent or Refractory Multiple Myeloma University Hospital Montpellier|Poitiers University Hospital April 23 2018 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo mice study?

Answer:
We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

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