Panobinostat (LBH589)

For research use only.

Catalog No.S1030 Synonyms: NVP-LBH589

289 publications

Panobinostat (LBH589) Chemical Structure

CAS No. 404950-80-7

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.

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Selleck's Panobinostat (LBH589) has been cited by 289 publications

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Biological Activity

Description Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Targets
HDAC (MOLT-4 cells) [1] HDAC (Reh cells) [1]
5 nM 20 nM
In vitro

LBH589 induces apoptosis among MOLT-4 and Reh cells in a time- and dose-dependent manner. Moreover, LBH589 is more potent in MOLT-4 than in Reh cells. LBH589 markedly prevents the growth of both MOLT-4 and Reh cells in a dose-dependent manner at 48 hours. LBH589 treatment causes a 2- to 3-fold increase in the number of cells in the G2/M phase of the cell cycle compared with the control cells. LBH589 is associated with induction of histone H3K9 and histone H4K8 acetylation as well as decreasing levels of c-Myc expression in a dose-dependent manner. LBH589 treatment also increases the levels of p21 expression. LBH589 treatment also decreases the levels of c-Myc after an initial increase at the lowest dose (10 nM) in Reh cells. In addition, LBH589 gives rise to substantial increases in mRNA levels of proapoptosis and DNA repair genes. LBH589 induces increased levels of acetylated histone H3 and H4 at the GADD45G promoter. [1] Besides, LBH589 inhibits growth of non small cell lung cancer cell lines (such as human H1299, L55 and A549 with IC50 of 5 nM, 11 nM and 30 nM, respectively), mesothelioma (such as human OK-6 and Ok-5 with IC50 of 5 nM and 7 nM, respectively) and small cell lung cancer cell lines (such as human RG-1 and LD-T with IC50 of 4 nM and 5 nM, respectively). [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT29 NXKxN2lRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\5eopVOC1zMDFOwG0> MUOwMVQh\A>? MlrObY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HepG2 MojRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTLb3UxNTFyIN88US=> MVSwMVQh\A>? NYDIWohvcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy Mnz4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HT29 MoP4SpVv[3Srb36gRZN{[Xl? NUjOS|d5PTEEoH7N NFrUNWMzPC15MjDo NUXzdVNtcW6mdXPl[EBi[3SrdnH0bY9vKG:oIHPhd5Bie2ViMzDh[pRmeiB2ONMgbOKh NHj4epU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewNlc5PCd-Mk[3NFI4QDR:L3G+
HepG2 MmPxSpVv[3Srb36gRZN{[Xl? NYXnUItPPTEEoH7N MWGyOE04OiCq NHG5c4VqdmS3Y3XkJIFkfGm4YYTpc44hd2ZiY3HzdIF{\SB|IHHmeIVzKDJ2wrDoxsA> MnPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HCC827 M{DTcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPpcXlbPS95LkWvNVAhdk1? MljjO|LDqGh? M{DxcGROW09? NFzjdYNmdmijbnPld{B1cGViYX70bZBzd2yrZnXyZZRqfmViZX\m[YN1KG:oIHXycI91cW6rYh?= NGf3VY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[3OVQ5PCd-Mk[2O|U1QDR:L3G+
A549  MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjRVVAyOC9zNT:yNEBvVQ>? MnPrO|LDqGh? M1XxTmROW09? M3TjN4VvcGGwY3XzJJRp\SCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5Qhd2ZiZYLsc5Rqdmmk M3L1S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nke1OFg1Lz5{Nk[3OVQ5PDxxYU6=
NCI-H460  NEDDXZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUGxNE8zOC9|MDDuUS=> NV[5SYUyPzMEoHi= MkDHSG1UVw>? Mo\o[Y5p[W6lZYOgeIhmKGGwdHnwdo9tcW[ncnH0bZZmKGWoZnXjeEBw\iCncnzveIlvcWJ? M4rETVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nke1OFg1Lz5{Nk[3OVQ5PDxxYU6=
J89GFP NFzWVIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXjNW9FVVORwrC= Mo[2SWM2OD12OT64OUDDuSBzMj62OUBvVQ>? Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkO1OlgoRjJ4NU[zOVY5RC:jPh?=
THP89GFP NIXWUpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jXOWROW00EoB?= NFfmeHJGSzVyPUG5MlM1KMLzIE[uOFMhdk1? NXnCTZFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlM2PjhpPkK2OVY{PTZ6PD;hQi=>
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G401 NX\kd3lqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDjNE4xOeLCk{GwMlAh|ryP MVSyOEBp NIPSUZFFVVORwrC= NVzaNZY1UUN3ME2xOFMvODJibl2= MkT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
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SK-NEP-1 MnLoRZBweHSxc3nzJGF{e2G7 NV7kSZlQPTBxMUCwJI5O Mnr6NlQhcA>? M{fuUWROW00EoB?= NIC4OWFqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ M13Q[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
G401 NVvOTpM3SXCxcITvd4l{KEG|c3H5 MVi1NE8yODBibl2= M4jJTFI1KGh? MYXEUXNQyqB? M1vOW4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NVHWS4lSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
SK-NEP-1 M1[4eWZ2dmO2aX;uJGF{e2G7 M4SxfVUxNzFyMDDuUS=> MWqyOEBp MoPvSG1UV8Li NV3aOIt2e2ixd4OgeIhmKGmwZIXjeIlwdiCxZjDEUmEh\nKjZ33lcpRifGmxbh?= NWfuU5NmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 Ml3RSpVv[3Srb36gRZN{[Xl? Ml\zOVAwOTByIH7N M2H4WlI1KGh? NIrqNndFVVORwrC= MnLVd4hwf3NidHjlJIlv\HWldHnvckBw\iCGTlGg[pJi\22nboTheIlwdg>? M{fZWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
SK-NEP-1 M325SWZ2dmO2aX;uJGF{e2G7 NVTjT4JPPTBxMUCwJI5O MoD3NlQhcA>? M2jKPWROW00EoB?= MoG2bY5lfWOnczDj[YxtKGO7Y3zlJIRqe2:{ZHXyxsA> MoLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
G401 M3;1RWZ2dmO2aX;uJGF{e2G7 MmT5OVAwOTByIH7N MmPhNlQhcA>? NWL1WYNUTE2VT9Mg MnPqbY5lfWOnczDj[YxtKGO7Y3zlJIRqe2:{ZHXyxsA> M17oPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
RPMI 8226 NFmyb2VE\WyuIGP1dpZqfmGuIFHzd4F6 M2K0elIwPC94IH7N MkXaOFjjiImq M1ftUYlv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? Mlz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
OPM2 MkfwR4VtdCCVdYL2bZZidCCDc4PhfS=> NYL4TGVnOi92L{[gcm0> NGfXblU1QOLCiXi= MY\pcoR2[2W|IHGgd4lodmmoaXPhcpQh\GWlcnXhd4UhcW5idHjlJINmdGxiZ4Lve5Rp M2HWdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwNlkzLz5{NkCwNFI6OjxxYU6=
U266 Ml7sR4VtdCCVdYL2bZZidCCDc4PhfS=> MlTwNk81NzZibl2= Ml;3OFjjiImq M17tUIlv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? M17XdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwNlkzLz5{NkCwNFI6OjxxYU6=
H929 NGLTPXdE\WyuIGP1dpZqfmGuIFHzd4F6 MWCyM|QwPiCwTR?= NVracoRDPDkkgJno Mo[xbY5lfWOnczDhJJNq\26rZnnjZY51KGSnY4LlZZNmKGmwIITo[UBk\WyuIHfyc5d1cA>? M{jXZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwNlkzLz5{NkCwNFI6OjxxYU6=
RPMI 8226  NImxSpVCeG:ydH;zbZMhSXO|YYm= MXq05qCKdk1? Mof2NlQwPDhiaB?= NGTpbGFqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IHnuJIEhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ Mmq1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
HCC827 NHHvTXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfWNVAhdk1? M4jWUVQ5KGh? Mly1SG1UVw>? NWDjeWk5\W6qYX7j[ZMh[2m|cHzheIlvKHOnboPpeIl3cXS7wrC= NY\XXZZ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OFQ3OTdpPkK1PVQ1PjF5PD;hQi=>
NCI-H23 NYPVZZU4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2W4VlExKG6P NV\0SlRvPDhiaB?= MlPvSG1UVw>? NV\xdGdE\W6qYX7j[ZMh[2m|cHzheIlvKHOnboPpeIl3cXS7wrC= MonPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NES2NVcoRjJ3OUS0OlE4RC:jPh?=
AML3 MkTxSpVv[3Srb36gRZN{[Xl? MVqwMVEh|ryP NEfuUmwzPMLiaB?= NVXZS3oxcW6mdXPld{BFVkFiZoLh[41mdnSjdHnvcuKhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1jlSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
ML-1 NYHZeHFQTnWwY4Tpc44hSXO|YYm= NHjO[pkxNTFizszN NEXFbZMzPMLiaB?= MmnwbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M3zLWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
RPMI-8226vr10  NX;4TotiTnWwY4Tpc44hSXO|YYm= MoLsNE0yKM7:TR?= NXPOe5dNOjUEoHi= NFnVRY9qdmS3Y3XzJGRPSSCocnHncYVvfGG2aX;uxsBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M4fzT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
ML-1 MlLMSpVv[3Srb36gRZN{[Xl? Mk\6NUDPxE1? NV\yfo5ZOjUEoHi= MlrGbY5kemWjc3XzJINie3Cjc3WtN{Bi[3Srdnn0fUA1NW[xbHS= NYfidWlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
RPMI-8226vr10  MXTGeY5kfGmxbjDBd5NigQ>? MoS1NUDPxE1? MnvhNlTDqGh? M2ToSIlv[3KnYYPld{Bk[XOyYYPlMVMh[WO2aY\peJkhOi53LX\vcIQ> MmG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MUK5OFEoRjJ3NkGyPVQyRC:jPh?=
SK-N-BE (2) M3[2fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\qTJUzPOLCiXi= M4PrZ2lEPTB;MUC0MlDjiIoEsfMAjVcvQCCwTR?= MmLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-BE (2), PAN  MK Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILVeFQzPOLCiXi= NFHLfWVKSzVyPUGwOE4x6oDLwsJihKk4Njhibl2= NEToW4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), MK  PAN NFfpSGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LTfFI16oDLaB?= NUDRdIFiUUN3ME2zPFIvOOLCidMx5qCKPDNwMjDuUS=> MoKwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-AS NYHJN5hMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPxbHAzPOLCiXi= MmmzTWM2OD1|Nz6x5qCKyrIkgJmyMlQhdk1? MnzXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-DZ NFLKSWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fvNFI16oDLaB?= NITUN3VKSzVyPUG3MlHjiIoEsfMAjVAvPCCwTR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV|MEi5NVY9N2F-
Caki-1 MnPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nYZ|ExNzJ3L{WwJI5O M1v2eVQ5KGh? MXjpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldkB{gW6ncnfpd5Rq[2GubImge4l1cCC{aYTvcoF3cXJ? MkjaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{mxPVEoRjJ3Mke5NVkyRC:jPh?=
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SK-N-BE NGT2boVHfW6ldHnvckBCe3OjeR?= NUT0SlV7OOLCk{SwJI5O MnK1OFghcA>? MmPKbY5lfWOnczDhJIRwe2VvZHXw[Y5l\W62IHPs[YF3[WenIH;mJINie3Cjc3WgN{BidmRiUFHSVC=> Ml3jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
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HepG2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TK[lEuOTByMDDuUS=> NIXtNFYzPC92OD:3NkBp NFTCTWVFVVOR M2XkeIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NUDuZldQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
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HCC-LM3 MlvHRZBweHSxc3nzJGF{e2G7 MkDLOVAhdk1? MXi0PEBp M332TGROW09? NHjxXXdqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IIPp[45q\mmlYX70cJkhcW5iYTDjZZNx[XOnLXTldIVv\GWwdDDtZY5v\XJiYomgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[ZMhOyxiODDhcoQhQQ>? NYC3VYE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
HepG2 M1;wWGFxd3C2b4Ppd{BCe3OjeR?= MWK1NEBvVQ>? NGrsT5U1QCCq NFz5WYdFVVOR MnrNbY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|aXfubYZq[2GwdHz5JIlvKGFiY3HzdIF{\S2mZYDlcoRmdnRibXHucoVzKGK7IHPs[YF3[WenIH;mJINie3Cjc3XzJFMtKDhiYX7kJFk> MlnVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 MW\BdI9xfG:|aYOgRZN{[Xl? Mmq2OVAhdk1? MWe0PEBp NHLDOVdFVVOR NFz1eVRqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IIPp[45q\mmlYX70cJkhcW5iYTDjZZNx[XOnLXTldIVv\GWwdDDtZY5v\XJiYomgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[ZMhOyxiODDhcoQhQQ>? MmTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HCC-LM3 M2HRO2Z2dmO2aX;uJGF{e2G7 NHX5VmU2OC9zMECgcm0> MWSyOEBp MoLGSG1UVw>? M1HTdIRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC= NUe5TVlnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
HepG2 MkXTSpVv[3Srb36gRZN{[Xl? MnTNOVAwOTByIH7N MYqyOEBp NGnKVJlFVVOR MnXx[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicD3TWGFVOyCjbnSgdE1Cc3UEoB?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
SMMC-7721 NEHhWHBHfW6ldHnvckBCe3OjeR?= NFrPOZM2OC9zMECgcm0> M4HwS|I1KGh? M2PGbWROW09? M1SxZYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
HCC-LM3 NEPTW2hHfW6ldHnvckBCe3OjeR?= MUW1NE8yODBibl2= NH3OSm0zPCCq MlLwSG1UVw>? NE\NPINld3ewcnXneYxifGW|IFLjcE15VCCneIDy[ZN{cW:w MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
HepG2 M1\GTmZ2dmO2aX;uJGF{e2G7 NXTuNXhVPTBxMUCwJI5O MWOyOEBp M3z3XmROW09? MmHZ[I94dnKnZ4XsZZRmeyCEY3ytfGwh\XiycnXzd4lwdg>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
SMMC-7721 M2Cx[WZ2dmO2aX;uJGF{e2G7 NX\zV5NlPTBxMUCwJI5O MX2yOEBp MUHEUXNQ M{LPWoRwf26{ZXf1cIF1\XNiQnPsMZhNKGW6cILld5Nqd25? NV:5cGxKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
FaDu MkfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnsNVAx6oDHbl2= NFLzR3Q5NzFyL{GyJIg> M4ezU4Rqe3CuYYnl[EBiKHOrZ37p[olk[W62IHHu[EBxem:ub37n[YQhTzJxTTDhdpJme3RiYYSgPEBidmRiMUNihKVpKHCxc4SgdoVt\WG|ZR?= M2rUflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK2OFgzLz5{NECyOlQ5OjxxYU6=
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LNCaP M1jxXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vk[lAuPSEQvF2= NVz3bY1mOjRxNEivO|IhcA>? NG\0eGJqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MlfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7OUGyNVYoRjJ|OUmxNlE3RC:jPh?=
RWPE-1  Ml;XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVj5PXFEOC1{MDFOwG0> MYiyOE81QC95MjDo MWHpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl7MUKxOkc,OjN7OUGyNVY9N2F-
Capan-1 MmLZSpVv[3Srb36gRZN{[Xl? M1\zeFI2NzVyL{GwNEBvVQ>? M4jhfVgwOjRxNEigbC=> MX7EUXNQ NI[4NHJld3ewcnXneYxifGWmIGLvckBuWk6DIHHu[EBxem:2ZXnuJIV5eHKnc4Ppc44h[W6mIHTve45{fHKnYX2gd4lodmGuaX7n NVmyUFEzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
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CFPAC-1  Mmf6SpVv[3Srb36gRZN{[Xl? MY[yOU82OC9zMECgcm0> MkCzPE8zPC92ODDo NFXlN3NFVVOR MV\kc5dvemWpdXzheIVlKFKxbjDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36gZY5lKGSxd37zeJJm[W1ic3nncoFtcW6p NXW0PHJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
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CFPAC-1  NETQfmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;pNlUwPTBxMUCwJI5O M4ToRVQ5KGh? NF;6T5ZFVVOR M3qzZpJm\HWlZYOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl{Mki4Okc,OjN7MkK4PFY9N2F-
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
DNMT1 / EZH2; 

PubMed: 19279403     


Western blot of DNMT1 and EZH2 in K562 and LAMA84 following 24 hours treatment with the indicated doses of panobinostat (PS). The levels of β-actin served as the loading control.

caspase-8 / cleaved caspase-8 / Sp1; 

PubMed: 27738323     


The indicated MM cell lines were cultured for 24 hours in the absence or presence of panobinostat at the indicated concentrations. Then, the protein levels of caspase-8, cleaved caspase 8 and Sp1 were analyzed by Western blotting. 

c-Myc / IRF4; 

PubMed: 27738323     


RPMI8226 cells were cultured for 24 hours in the absence or presence of panobinostat at the indicated concentrations. Then, the protein levels of cMyc and IRF4 were analyzed by Western blotting.

Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1; 

PubMed: 28915627     


Immunoblotting of whole cell lysates prepared from MB cells treated with panobinostat. The representative western blot result shows panobinostat clearly regulated acetyl-Histone H3 (Ac-H3), cleaved caspase-3, CCND1, IDs, synaptophysin and NeuroD1.

RAD51 / BRCA1 / CHK1 / RPL13a; 

PubMed: 24244429     


CTS, OCI-AML3 or U937 AML cells were treated with variable concentrations of panobinostat for 48 h. Whole cell lysates were subjected to Western blotting to measure protein levels for BRCA1, CHK1, and RAD51 in the cells.

H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP; 

PubMed: 31071955     


Immunoblot analyses of histone acetylation (H3K9AC, H3K18AC, H3K56AC, H4K8AC and H4K16AC), cell cycle arrest (p27 and p27) and apoptotic-related protein (C-Caspase 3 and C-PARP) expression following various panobinostat treatments for 6 h and 24 h on K562 cells. H3, H4 and GAPDH immunoblots served as internal controls.

19279403 27738323 28915627 24244429 31071955
Immunofluorescence
Synaptophysin / NACM; 

PubMed: 28915627     


Panobinostat treated cells are positive for (B) synaptophysin (red) and (C) NCAM (red). Scale bars, 50 μm.

α-tubulin / Acetyl-α-tubulin; 

PubMed: 29983882     


CLBL-1 cells were treated with 10 nM of panobinostat for 24 h and then microtubules were visualized by immunofluorescence labeling using antibodies against tubulin and acetylated tubulin (ac-tubulin) using the appropriate excitation and emission filters as described in the material and methods section. Representative microphotographs with tubulin and ac-tubulin labeling (green) and DAPI stained-nuclei (blue) at 100× magnification are shown. Scale bar, 5 μm.

BiP; 

PubMed: 23544167     


Immunofluorescence analysis of BiP after 24 and 48 hours of treatment in HepG2 (upper panel) and Hep3B (lower panel) cells with 0.1 µM panobinostat and 10 nM thapsigargin, showing an increase and a different protein distribution in panobinostat-treated cells, comparable to 10 nM thapsigargin effect, used as a positive control. Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is x630, and scale bar represents 10 µm.

ATF4; 

PubMed: 23544167     


HepG2 (left panel) and Hep3B (right panel) cells were treated for 24 and 48 hours with 0.1 µM panobinostat and the immunofluorescence results show an increase of ATF4 and its nuclear localization in both cell lines. Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is x630, and scale bar represents 10 µm.

IRE1α / S724-IRE1α; 

PubMed: 23544167     


Analysis of IRE1α/XBP1 involvement. Immunofluorescence results of IRE1α and its phosphorylated form in HepG2 (A) and Hep3B (B) cell lines after 24 and 48 hours of treatment with 0.1 µM panobinostat. The results indicate that panobinostat induces an increase of both the total and the phosphorylated form of IRE1α and a spotted distribution of this protein (enlargement showed for phospho-IRE1α, B). Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is x630, and scale bar represents 10 µm.

28915627 29983882 23544167
Growth inhibition assay
Cell viability; 

PubMed: 27738323     


The indicated MM cell lines were cultured in triplicate in the absence or presence of panobinostat at the indicated concentrations. After culturing for 48 hours, cell viability was measured by a WST-8 cell proliferation assay. Results were expressed as the mean +/− SD.

27738323
In vivo In lung cancer and mesothelioma animal models, LBH589 markedly decreases tumor growth by 62%. LBH589 is equally effective in immunocompetent and severe combined immunodeficien-cymice, suggesting that the inhibition of tumor growth by LBH589 is not due to direct immunologic effects. Daily LBH589, given i.p. at 20 mg/kg for 5 days per week, leading to an average decrease in growth of 70%. Compared with the corresponding control tumors, LBH589 leads to a 53% decrease for H526-derived tumors, an 81% decrease for BK-T-derived tumors, a 76% decrease for RG-1- derived tumors, and a 70% decrease for H69-derived tumors. In contrast to the lack of tumor regression notes in NSCLC and Meso-derived xenografted tumors that are treated under identical conditions and doses, LBH589 results in dramatic tumor regression in SCLC-derived tumors and RG-1-derived tumor. [2]

Protocol

Cell Research:[1]
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  • Cell lines: MOLT-4 cell lines and Reh (pre-B cells)
  • Concentrations: 50 nM
  • Incubation Time: 48 hours
  • Method: Untreated and LBH589-treated cells [human Ph- acute lymphoblastic leukemia MOLT-4 (T cells) and Reh (pre-B cells)] are stained with annexin V and propidium iodide using annexin V-FITC apoptosis detection kit I. The percentage of apoptotic and nonviable cells is determined by flow cytometry. At least 5 × 104 cells are collected with a CyAn ADP Violet cytometer. Percentage apoptosis is calculated considering all the annexin V-positive plus the annexin V/PI-positive cells; percentage loss of cell viability is calculated considering all the annexin V-positive plus the PI-positive and the annexinV/PI-positive cells.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Severe combined immunodeficiency (SCID) mice with M30 (107 cells) or A549 (5 × 106 cells), H69 (2.5 × 106 cells), BK-T (6.5 × 106), H526 (10 × 106), and RG1 (10 × 106) cells
  • Dosages: 10 mg/kg, 20 mg/kg
  • Administration: Administered via i.p. injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 69 mg/mL (197.46 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+48% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 349.43
Formula

C21H23N3O2

CAS No. 404950-80-7
Storage powder
in solvent
Synonyms NVP-LBH589
Smiles CC1=C(C2=CC=CC=C2N1)CCNCC3=CC=C(C=C3)C=CC(=O)NO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04341311 Recruiting Drug: Marizomib|Drug: Panobinostat Diffuse Intrinsic Pontine Glioma|Pediatric Brainstem Glioma|Pediatric Brainstem Gliosarcoma Recurrent|Pediatric Cancer|Pediatric Brain Tumor|Diffuse Glioma Dana-Farber Cancer Institute|Celgene|Secura Bio August 10 2020 Phase 1
NCT03632317 Withdrawn Drug: Panobinostat|Drug: Everolimus Glioma|Diffuse Intrinsic Pontine Glioma University of Michigan Rogel Cancer Center October 2019 Phase 2
NCT03982134 Withdrawn Drug: PDR001|Drug: Panobinostat Melanoma|Non Small Cell Lung Cancer Muhammad Furqan|Novartis Pharmaceuticals|University of Iowa September 2019 Phase 1
NCT04326764 Recruiting Drug: Panobinostat Acute Myeloid Leukaemia (AML)|Myelodysplastic Syndromes (MDS) Goethe University|Jan J. Cornelissen (HOVON-SAKK)|Sebastian Giebel (PALG) July 24 2018 Phase 3
NCT03515915 Unknown status -- Patients With Recurrent or Refractory Multiple Myeloma University Hospital Montpellier|Poitiers University Hospital April 23 2018 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to reconstitute the compound for in vivo mice study?

  • Answer:

    We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID