Panobinostat (LBH589)

Catalog No.S1030 Synonyms: NVP-LBH589

For research use only.

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.

Panobinostat (LBH589) Chemical Structure

CAS No. 404950-80-7

Selleck's Panobinostat (LBH589) has been cited by 336 publications

Purity & Quality Control

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Biological Activity

Description Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Targets
HDAC (MOLT-4 cells) [1] HDAC (Reh cells) [1]
5 nM 20 nM
In vitro

LBH589 induces apoptosis among MOLT-4 and Reh cells in a time- and dose-dependent manner. Moreover, LBH589 is more potent in MOLT-4 than in Reh cells. LBH589 markedly prevents the growth of both MOLT-4 and Reh cells in a dose-dependent manner at 48 hours. LBH589 treatment causes a 2- to 3-fold increase in the number of cells in the G2/M phase of the cell cycle compared with the control cells. LBH589 is associated with induction of histone H3K9 and histone H4K8 acetylation as well as decreasing levels of c-Myc expression in a dose-dependent manner. LBH589 treatment also increases the levels of p21 expression. LBH589 treatment also decreases the levels of c-Myc after an initial increase at the lowest dose (10 nM) in Reh cells. In addition, LBH589 gives rise to substantial increases in mRNA levels of proapoptosis and DNA repair genes. LBH589 induces increased levels of acetylated histone H3 and H4 at the GADD45G promoter. [1] Besides, LBH589 inhibits growth of non small cell lung cancer cell lines (such as human H1299, L55 and A549 with IC50 of 5 nM, 11 nM and 30 nM, respectively), mesothelioma (such as human OK-6 and Ok-5 with IC50 of 5 nM and 7 nM, respectively) and small cell lung cancer cell lines (such as human RG-1 and LD-T with IC50 of 4 nM and 5 nM, respectively). [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT29 NHj6[lhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPMNE0yOCEQvF2= MXOwMVQh\A>? MoLqbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HepG2 NGXLTWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHldY4xNTFyIN88US=> NGLMO2wxNTRiZB?= NYTuXZNwcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M3raT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{CyO|g1Lz5{NkewNlc5PDxxYU6=
HT29 NX3lfGtpTnWwY4Tpc44hSXO|YYm= M4TjelUxyqCwTR?= MVWyOE04OiCq MnG5bY5lfWOnZDDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UhOyCjZoTldkA1QMLiaNMg NW\Xd2U5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFI4QDRpPkK2O|AzPzh2PD;hQi=>
HepG2 MnT3SpVv[3Srb36gRZN{[Xl? MlnTOVDDqG6P NXPSNnJjOjRvN{KgbC=> MUHpcoR2[2WmIHHjeIl3[XSrb36gc4Yh[2G|cHHz[UA{KGGodHXyJFI1yqCqwrC= MlzYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HCC827 NUWwRpUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzqPYQ2NzdwNT:xNEBvVQ>? NUnLZXkxPzMEoHi= NU\1TYxETE2VTx?= NV64Z3JV\W6qYX7j[ZMhfGinIHHueIlxem:uaX\ldoF1cX[nIHXm[oVkfCCxZjDldoxwfGmwaXK= NYXtb4tMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2O|U1QDRpPkK2Olc2PDh2PD;hQi=>
A549  NEPuO|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjERW9oOTBxMUWvNlAhdk1? NYXOVoJSPzMEoHi= M1vySmROW09? M2XLZYVvcGGwY3XzJJRp\SCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5Qhd2ZiZYLsc5Rqdmmk MmrPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4N{W0PFQoRjJ4Nke1OFg1RC:jPh?=
NCI-H460  M3GwSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fzb|ExNzJyL{OwJI5O M{iyPFczyqCq NF:0e4lFVVOR NWrwXI9b\W6qYX7j[ZMhfGinIHHueIlxem:uaX\ldoF1cX[nIHXm[oVkfCCxZjDldoxwfGmwaXK= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZ5NUS4OEc,OjZ4N{W0PFQ9N2F-
J89GFP NYnlXGRHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\tZWROW00EoB?= MULFR|UxRTR7Lki1JOKyKDF{Lk[1JI5O M3POS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NU[zOVY5Lz5{NkW2N|U3QDxxYU6=
THP89GFP M3HGN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXUd3RDTE2VT9Mg MXvFR|UxRTF7LkO0JOKyKDZwNEOgcm0> NFPZeXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2N|U3QCd-Mk[1OlM2Pjh:L3G+
SK-NEP-1 NXjKZoFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVqwMlAy6oDVMUCuNEDPxE1? NH[4TFQzPCCq NGrmWFRFVVORwrC= NYnEdY1zUUN3ME23Ok4{PCCwTR?= NUCzSJRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 M2LqVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{P2PFAvODIkgKOxNE4xKM7:TR?= MnzONlQhcA>? NFPve3FFVVORwrC= M4nzOWlEPTB;MUSzMlAzKG6P NF[2OG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
SK-NEP-1 NEPKcGJE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1O3N|UxKG6P MUGx5qCUPCCm M4Xp[2ROW00EoB?= NXjXS4o3emWmdXPld{Bk\WyuIIP1dpZqfmGuIHnuJIEhfGmvZTDk[ZBmdmSnboSgcYFvdmW{ MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 NGXSNXRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXPtPXI{PTBibl2= MXyx5qCUPCCm MYLEUXNQyqB? M3vzXpJm\HWlZYOgZ4VtdCC|dYL2bZZidCCrbjDhJJRqdWViZHXw[Y5l\W62IH3hco5meg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
SK-NEP-1 NWC1b5VVSXCxcITvd4l{KEG|c3H5 M{Lad|UxNzFyMDDuUS=> NEHzOJczPCCq NHm1fFhFVVORwrC= MkjtbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? Mk\6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
G401 Mm\6RZBweHSxc3nzJGF{e2G7 MkizOVAwOTByIH7N NWfoeG1ZOjRiaB?= NUKybHhXTE2VT9Mg NVm5fpJRcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NGHCWlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
SK-NEP-1 M3\3XGZ2dmO2aX;uJGF{e2G7 NFrUUI02OC9zMECgcm0> NVHMUmF7OjRiaB?= M2TyWmROW00EoB?= NFjQe5R{cG:5czD0bIUhcW6mdXP0bY9vKG:oIFTORUBnemGpbXXueIF1cW:w MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 MYTGeY5kfGmxbjDBd5NigQ>? NG\lPWE2OC9zMECgcm0> MY[yOEBp NXT0[XFQTE2VT9Mg M4XEVZNpd3e|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25? NInGUY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
SK-NEP-1 MnvKSpVv[3Srb36gRZN{[Xl? M4LBSVUxNzFyMDDuUS=> NGewdZYzPCCq NX:yenV5TE2VT9Mg MUXpcoR2[2W|IHPlcIwh[3mlbHWg[Il{d3KmZYNCpC=> MlfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
G401 M{fNdWZ2dmO2aX;uJGF{e2G7 MYG1NE8yODBibl2= NFH6bHozPCCq Ml3YSG1UV8Li MoiybY5lfWOnczDj[YxtKGO7Y3zlJIRqe2:{ZHXyxsA> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
RPMI 8226 M33FPGNmdGxiU4Xyeol3[WxiQYPzZZk> MnT5Nk81NzZibl2= MlnVOFjjiImq NHm3UG5qdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq MonKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
OPM2 MlvHR4VtdCCVdYL2bZZidCCDc4PhfS=> M1\tclIwPC94IH7N MkXIOFjjiImq NEW1TYdqdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq NF7mXm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCwNFI6Oid-Mk[wNFAzQTJ:L3G+
U266 NHnWfINE\WyuIGP1dpZqfmGuIFHzd4F6 NX;4OYdSOi92L{[gcm0> NYXDZ|k1PDkkgJno M2nZc4lv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? NI\hZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCwNFI6Oid-Mk[wNFAzQTJ:L3G+
H929 NHTMNHZE\WyuIGP1dpZqfmGuIFHzd4F6 NYXaUFh6Oi92L{[gcm0> MmPWOFjjiImq M1HqcYlv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? NV3ZTJRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
RPMI 8226  MVTBdI9xfG:|aYOgRZN{[Xl? Mn3JOQKBkW6P NHnseoczPC92ODDo MlLYbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? NGXWc5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCwNFI6Oid-Mk[wNFAzQTJ:L3G+
HCC827 NYTTTWF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXexNEBvVQ>? M3[2N|Q5KGh? NFTMVI1FVVOR MlPa[Y5p[W6lZYOgZ4l{eGyjdHnuJJNmdnOrdHn2bZR6yqB? NH3vV3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0OFYyPyd-MkW5OFQ3OTd:L3G+
NCI-H23 MkLwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnWNVAhdk1? MUW0PEBp MX3EUXNQ Mnn5[Y5p[W6lZYOgZ4l{eGyjdHnuJJNmdnOrdHn2bZR6yqB? MljhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NES2NVcoRjJ3OUS0OlE4RC:jPh?=
AML3 MYHGeY5kfGmxbjDBd5NigQ>? M3fSTFAuOSEQvF2= NWDwVnQ2OjUEoHi= NF;EbJJqdmS3Y3XzJGRPSSCocnHncYVvfGG2aX;uxsBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M1LndFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
ML-1 M{HxXGZ2dmO2aX;uJGF{e2G7 NH;HS4cxNTFizszN NYL1XFVVOjUEoHi= MVfpcoR2[2W|IFTORUBnemGpbXXueIF1cW:wwrDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M2rXWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
RPMI-8226vr10  Mn7wSpVv[3Srb36gRZN{[Xl? NHKwOHgxNTFizszN NXHBeYtuOjUEoHi= NGixW2ZqdmS3Y3XzJGRPSSCocnHncYVvfGG2aX;uxsBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NUjaVW5{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
ML-1 M33DbWZ2dmO2aX;uJGF{e2G7 NVv3R5BnOSEQvF2= MWCyOOKhcA>? MWfpcoNz\WG|ZYOgZ4F{eGG|ZT2zJIFkfGm4aYT5JFQu\m:uZB?= M{LrRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
RPMI-8226vr10  M13qeWZ2dmO2aX;uJGF{e2G7 MkjDNUDPxE1? M4LtcFI1yqCq MojQbY5kemWjc3XzJINie3Cjc3WtN{Bi[3Srdnn0fUAzNjVvZn;s[C=> NXztXY83RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
SK-N-BE (2) M2r3[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYmyOQKBkWh? MWPJR|UxRTFyND6w5qCKyrIkgJm3Mlghdk1? NFzMVpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), PAN  MK NFjYOYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGX3do0zPOLCiXi= M1zwNGlEPTB;MUC0MlDjiIoEsfMAjVcvQCCwTR?= NYn5XVl[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N|A5QTF4PD;hQi=>
SK-N-BE (2), MK  PAN NFLkNYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fOUFI16oDLaB?= M{fNemlEPTB;M{iyMlDjiIoEsfMAjVQ{NjJibl2= MkjDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-AS M{C5eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\aOYozPOLCiXi= Mmi0TWM2OD1|Nz6x5qCKyrIkgJmyMlQhdk1? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV|MEi5NVY9N2F-
SK-N-DZ MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XPPFI16oDLaB?= M3;IV2lEPTB;MUeuNgKBkcLz4pEJNE41KG6P MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV|MEi5NVY9N2F-
Caki-1 NGrO[plIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3exXFExNzJ3L{WwJI5O MVe0PEBp NF\xd5VqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meiC|eX7ldodqe3SrY3HscJkhf2m2aDDybZRwdmG4aYK= M1fkbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke5NVkyLz5{NUK3PVE6OTxxYU6=
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SK-N-DZ M1HPNmZ2dmO2aX;uJGF{e2G7 M{PIZlDjiJN6MDDuUS=> M3XM[FQ5KGh? M2DPOYlv\HWlZYOgZUBld3OnLXTldIVv\GWwdDDjcIVifmGpZTDv[kBk[XOyYYPlJFMh[W6mIGDBVnA> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEe5PUc,OjRyOUi3PVk9N2F-
SK-N-SH MlfDSpVv[3Srb36gRZN{[Xl? MV:w5qCUPDBibl2= MWC0PEBp MXPpcoR2[2W|IHGg[I9{\S2mZYDlcoRmdnRiY3zlZZZi\2Vib3[gZ4F{eGG|ZTCzJIFv\CCSQWLQ MljqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
SK-N-BE MnziSpVv[3Srb36gRZN{[Xl? M4DldFDjiJN2MDDuUS=> M{PsblQ5KGh? MYHpcoR2[2W|IHGg[I9{\S2mZYDlcoRmdnRiY3zlZZZi\2Vib3[gZ4F{eGG|ZTCzJIFv\CCSQWLQ NUW0VFV[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg4QTlpPkK0NFk5Pzl7PD;hQi=>
HCC-LM3 NUP4[WZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPWNU0yODByIH7N M1fFUFI1NzR6L{eyJIg> MlfTSG1UVw>? NH;FS|VqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
HepG2 M2q2dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1q0d|EuOTByMDDuUS=> MlXyNlQwPDhxN{KgbC=> NVGyTI5VTE2VTx?= NWPiT|VkcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy Mme4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 M4jHXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTRem5FOS1zMECwJI5O M3u2eVI1NzR6L{eyJIg> MUXEUXNQ MnT1bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ Ml3LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HCC-LM3 NF3Id41CeG:ydH;zbZMhSXO|YYm= MVi1NEBvVQ>? M3G1SFQ5KGh? MWfEUXNQ MkTkbY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|aXfubYZq[2GwdHz5JIlvKGFiY3HzdIF{\S2mZYDlcoRmdnRibXHucoVzKGK7IHPs[YF3[WenIH;mJINie3Cjc3XzJFMtKDhiYX7kJFk> Mly5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HepG2 M1LDdGFxd3C2b4Ppd{BCe3OjeR?= MV[1NEBvVQ>? MljhOFghcA>? M2XJcGROW09? M3vFdolv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3nncolncWOjboTsfUBqdiCjIHPhd5Bie2VvZHXw[Y5l\W62IH3hco5meiCkeTDjcIVifmGpZTDv[kBk[XOyYYPld{A{NCB6IHHu[EA6 MlXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 M4fNdGFxd3C2b4Ppd{BCe3OjeR?= MmHVOVAhdk1? M1faUVQ5KGh? NYj3UpFFTE2VTx?= MWnpcoR2[2W|IHPlcIwh[XCxcITvd4l{KHOrZ37p[olk[W62bImgbY4h[SClYYPwZZNmNWSncHXu[IVvfCCvYX7u[ZIh[nliY3zlZZZi\2Vib3[gZ4F{eGG|ZYOgN{whQCCjbnSgPS=> M4HaN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
HCC-LM3 MYjGeY5kfGmxbjDBd5NigQ>? NFvJXok2OC9zMECgcm0> MXqyOEBp MXHEUXNQ M3SyXYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC= MkLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HepG2 MX;GeY5kfGmxbjDBd5NigQ>? MYS1NE8yODBibl2= M{HqV|I1KGh? NWrYWW9rTE2VTx?= Mlm3[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicD3TWGFVOyCjbnSgdE1Cc3UEoB?= MoexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 M4DVbGZ2dmO2aX;uJGF{e2G7 MXe1NE8yODBibl2= NXOzOHR7OjRiaB?= Mn7TSG1UVw>? NUnNOnU2\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdE1UXEGWMzDhcoQheC2Da4VCpC=> NF6xVGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3 NVTsZ3lRTnWwY4Tpc44hSXO|YYm= NYXDfHpIPTBxMUCwJI5O MXqyOEBp NHvHWodFVVOR NX;1VVVJ\G:5boLl[5Vt[XSnczDCZ4wugExiZYjwdoV{e2mxbh?= NFnMbIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HepG2 NIXUVnBHfW6ldHnvckBCe3OjeR?= MVW1NE8yODBibl2= NHvpS4czPCCq NIfr[21FVVOR MXPkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v MkXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 M4HNdGZ2dmO2aX;uJGF{e2G7 NIGwd4U2OC9zMECgcm0> MmfwNlQhcA>? MoK5SG1UVw>? MYLkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v MnnUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
FaDu NIf6UnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWexNFDjiIWwTR?= M4nYOlgwOTBxMUKgbC=> MnK1[Il{eGyjeXXkJIEhe2mpbnnmbYNidnRiYX7kJJBzd2yxbnfl[EBIOi:PIHHydoV{fCCjdDC4JIFv\CBzMvMAiYgheG:|dDDy[Yxm[XOn MlG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMk[0PFIoRjJ2MEK2OFgzRC:jPh?=
FaDu NISzdphHfW6ldHnvckBCe3OjeR?= NVnpc4o5OTBy4pEFcm0> NEHkTo4zNzRxOD:xNkBp MVfpcoR2[2WmIICyNXdi\jFxQ3nwNeKh\XiycnXzd4lwdg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB{NkS4Nkc,OjRyMk[0PFI9N2F-
PC-3  MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWOwMVExKM7:TR?= MmrGNlQwPDhxN{KgbC=> M3nUVYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NEHQblU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m5NVIyPid-MkO5PVEzOTZ:L3G+
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RWPE-1  M2PKb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmn1NE0zOCEQvF2= M3zsU|I1NzR6L{eyJIg> MmfjbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl7MUKxOkc,OjN7OUGyNVY9N2F-
Capan-1 NU\GPVdrTnWwY4Tpc44hSXO|YYm= NXWyV2lsOjVxNUCvNVAxKG6P M2DlblgwOjRxNEigbC=> Ml\aSG1UVw>? NYHFVoNH\G:5boLl[5Vt[XSnZDDSc44hdVKQQTDhcoQheHKxdHXpckBmgHC{ZYPzbY9vKGGwZDDkc5dve3S{ZXHtJJNq\26jbHnu[y=> NUTOXpdXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
L3.6pl M1rzemZ2dmO2aX;uJGF{e2G7 MWCyOU82OC9zMECgcm0> NUPvV2lOQC9{ND:0PEBp NHHY[GtFVVOR M2L5OYRwf26{ZXf1cIF1\WRiUn;uJI1TVkFiYX7kJJBzd3SnaX6g[ZhxemW|c3nvckBidmRiZH;3cpN1emWjbTDzbYdv[Wyrbne= Mk\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ|OUKyPFg3RC:jPh?=
CFPAC-1  MlfhSpVv[3Srb36gRZN{[Xl? MVqyOU82OC9zMECgcm0> MYO4M|I1NzR6IHi= M4PWSmROW09? M3\nZoRwf26{ZXf1cIF1\WRiUn;uJI1TVkFiYX7kJJBzd3SnaX6g[ZhxemW|c3nvckBidmRiZH;3cpN1emWjbTDzbYdv[Wyrbne= NF\OO3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{myNlg5Pid-MkO5NlI5QDZ:L3G+
Capan-1 MoTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnBOGV4OjVxNUCvNVAxKG6P Ml\aOFghcA>? NVzZcnpUTE2VTx?= MUPy[YR2[2W|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MlH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ|OUKyPFg3RC:jPh?=
L3.6pl MoLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v3flI2NzVyL{GwNEBvVQ>? NEnKfpU1QCCq MVfEUXNQ M2niTZJm\HWlZYOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> Ml72QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ|OUKyPFg3RC:jPh?=
CFPAC-1  M1fQSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fjXlI2NzVyL{GwNEBvVQ>? NEjZR4k1QCCq NHGz[HBFVVOR NGTmXpFz\WS3Y3XzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NGL4ToU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{myNlg5Pid-MkO5NlI5QDZ:L3G+
Capan-1 MXfBdI9xfG:|aYOgRZN{[Xl? M2r0RVI2NzVyL{GwNEBvVQ>? NWK0[3NoPDhiaB?= M{TJeWROW09? MnnDbY5lfWOnczDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NYrkNWVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
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CFPAC-1  M17xbmFxd3C2b4Ppd{BCe3OjeR?= MVuyOU82OC9zMECgcm0> M2H2XlQ5KGh? MkSwSG1UVw>? MX;pcoR2[2W|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NYDhSmRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
HN22 M1XOcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\MbIl4OC1{MDDuUS=> NWnldYtOOjRxNEigbC=> M3H3T2ROW09? NF7nTZRqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIITpcYUuKGGwZDDkc5NmNSCmZYDlcoRmdnRibXHucoVz NEK5SlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3O|I{PSd-MkO4O|czOzV:L3G+
HSC4  NVHpS5NKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX:wMVIxKG6P NEW0RZEzPC92ODDo NFHrNVBFVVOR MYjpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMUBl\XCnbnTlcpQhdWGwbnXy MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HN22 NY\HO3p{SXCxcITvd4l{KEG|c3H5 NYjWRo5POC1{MDDuUS=> MXK0PEBp MonjSG1UVw>? MoPZbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MkC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{eyN|UoRjJ|OEe3NlM2RC:jPh?=
HSC4  MXfBdI9xfG:|aYOgRZN{[Xl? NXnPdlBrOC1{MDDuUS=> M2PXVFQ5KGh? Mmi5SG1UVw>? MnPMbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? M3XDclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe3NlM2Lz5{M{i3O|I{PTxxYU6=
HN22 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHQOYMxNTJyIH7N Mn3HOFghcA>? MVHEUXNQ NV;IVZlqcW6mdXPld{BIOSCyaHHz[UBk\WyuIHP5Z4xmKGG{cnXzeOKh MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HSC4  NIn3TWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLwS3ZkOC1{MDDuUS=> Mkn0OFghcA>? NIX1dGVFVVOR NHfxW5ZqdmS3Y3XzJGcyKHCqYYPlJINmdGxiY4njcIUh[XK{ZYP0xsA> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HN22 M37Jb2Z2dmO2aX;uJGF{e2G7 MViwMVIxKG6P MVi0PEBp M{XHVmROW09? MXjzeZBxemW|c3XzJHNxOSCneIDy[ZN{cW:wwrC= M1TpXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe3NlM2Lz5{M{i3O|I{PTxxYU6=
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Assay
Methods Test Index PMID
Western blot DNMT1 / EZH2 ; caspase-8 / cleaved caspase-8 / Sp1 ; c-Myc / IRF4 ; Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1 ; RAD51 / BRCA1 / CHK1 / RPL13a ; H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP 19279403 27738323 28915627 24244429 31071955
Immunofluorescence Synaptophysin / NACM ; α-tubulin / Acetyl-α-tubulin ; BiP ; ATF4 ; IRE1α / S724-IRE1α 28915627 29983882 23544167
Growth inhibition assay Cell viability 27738323
In vivo In lung cancer and mesothelioma animal models, LBH589 markedly decreases tumor growth by 62%. LBH589 is equally effective in immunocompetent and severe combined immunodeficien-cymice, suggesting that the inhibition of tumor growth by LBH589 is not due to direct immunologic effects. Daily LBH589, given i.p. at 20 mg/kg for 5 days per week, leading to an average decrease in growth of 70%. Compared with the corresponding control tumors, LBH589 leads to a 53% decrease for H526-derived tumors, an 81% decrease for BK-T-derived tumors, a 76% decrease for RG-1- derived tumors, and a 70% decrease for H69-derived tumors. In contrast to the lack of tumor regression notes in NSCLC and Meso-derived xenografted tumors that are treated under identical conditions and doses, LBH589 results in dramatic tumor regression in SCLC-derived tumors and RG-1-derived tumor. [2]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: MOLT-4 cell lines and Reh (pre-B cells)
  • Concentrations: 50 nM
  • Incubation Time: 48 hours
  • Method: Untreated and LBH589-treated cells [human Ph- acute lymphoblastic leukemia MOLT-4 (T cells) and Reh (pre-B cells)] are stained with annexin V and propidium iodide using annexin V-FITC apoptosis detection kit I. The percentage of apoptotic and nonviable cells is determined by flow cytometry. At least 5 × 104 cells are collected with a CyAn ADP Violet cytometer. Percentage apoptosis is calculated considering all the annexin V-positive plus the annexin V/PI-positive cells; percentage loss of cell viability is calculated considering all the annexin V-positive plus the PI-positive and the annexinV/PI-positive cells.
Animal Research:[2]
  • Animal Models: Severe combined immunodeficiency (SCID) mice with M30 (107 cells) or A549 (5 × 106 cells), H69 (2.5 × 106 cells), BK-T (6.5 × 106), H526 (10 × 106), and RG1 (10 × 106) cells
  • Dosages: 10 mg/kg, 20 mg/kg
  • Administration: Administered via i.p. injection

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+48% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 349.43
Formula

C21H23N3O2

CAS No. 404950-80-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C2=CC=CC=C2N1)CCNCC3=CC=C(C=C3)C=CC(=O)NO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04341311 Active not recruiting Drug: Marizomib|Drug: Panobinostat Diffuse Intrinsic Pontine Glioma|Pediatric Brainstem Glioma|Pediatric Brainstem Gliosarcoma Recurrent|Pediatric Cancer|Pediatric Brain Tumor|Diffuse Glioma Dana-Farber Cancer Institute|Celgene|Secura Bio Inc. August 10 2020 Phase 1
NCT03632317 Withdrawn Drug: Panobinostat|Drug: Everolimus Glioma|Diffuse Intrinsic Pontine Glioma University of Michigan Rogel Cancer Center October 2019 Phase 2
NCT03982134 Withdrawn Drug: PDR001|Drug: Panobinostat Melanoma|Non Small Cell Lung Cancer Muhammad Furqan|Novartis Pharmaceuticals|University of Iowa September 2019 Phase 1
NCT04326764 Active not recruiting Drug: Panobinostat Acute Myeloid Leukaemia (AML)|Myelodysplastic Syndromes (MDS) Goethe University|Jan J. Cornelissen (HOVON-SAKK)|Sebastian Giebel (PALG) July 24 2018 Phase 3
NCT03515915 Unknown status -- Patients With Recurrent or Refractory Multiple Myeloma University Hospital Montpellier|Poitiers University Hospital April 23 2018 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo mice study?

Answer:
We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

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