Panobinostat (LBH589)

For research use only.

Catalog No.S1030 Synonyms: NVP-LBH589

313 publications

Panobinostat (LBH589) Chemical Structure

CAS No. 404950-80-7

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.

Selleck's Panobinostat (LBH589) has been cited by 313 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Targets
HDAC (MOLT-4 cells) [1] HDAC (Reh cells) [1]
5 nM 20 nM
In vitro

LBH589 induces apoptosis among MOLT-4 and Reh cells in a time- and dose-dependent manner. Moreover, LBH589 is more potent in MOLT-4 than in Reh cells. LBH589 markedly prevents the growth of both MOLT-4 and Reh cells in a dose-dependent manner at 48 hours. LBH589 treatment causes a 2- to 3-fold increase in the number of cells in the G2/M phase of the cell cycle compared with the control cells. LBH589 is associated with induction of histone H3K9 and histone H4K8 acetylation as well as decreasing levels of c-Myc expression in a dose-dependent manner. LBH589 treatment also increases the levels of p21 expression. LBH589 treatment also decreases the levels of c-Myc after an initial increase at the lowest dose (10 nM) in Reh cells. In addition, LBH589 gives rise to substantial increases in mRNA levels of proapoptosis and DNA repair genes. LBH589 induces increased levels of acetylated histone H3 and H4 at the GADD45G promoter. [1] Besides, LBH589 inhibits growth of non small cell lung cancer cell lines (such as human H1299, L55 and A549 with IC50 of 5 nM, 11 nM and 30 nM, respectively), mesothelioma (such as human OK-6 and Ok-5 with IC50 of 5 nM and 7 nM, respectively) and small cell lung cancer cell lines (such as human RG-1 and LD-T with IC50 of 4 nM and 5 nM, respectively). [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT29 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHi2[Y8xNTFyIN88US=> NGf0SlMxNTRiZB?= M1fPWYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= Mn3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HepG2 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rjdlAuOTBizszN NVLkPZd7OC12IHS= MmP4bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NWjwfmJnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3NFI4QDRpPkK2O|AzPzh2PD;hQi=>
HT29 NULueZRWTnWwY4Tpc44hSXO|YYm= MVO1NOKhdk1? NVy1foFWOjRvN{KgbC=> M4fYbIlv\HWlZXSgZYN1cX[jdHnvckBw\iClYYPwZZNmKDNiYX\0[ZIhPDkEoHlCpC=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HepG2 Mn;1SpVv[3Srb36gRZN{[Xl? MnjhOVDDqG6P MX2yOE04OiCq MknTbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UhOyCjZoTldkAzPMLiaNMg MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HCC827 NGW0U|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnrc|M2NzdwNT:xNEBvVQ>? NEjtb5g4OsLiaB?= Ml3uSG1UVw>? NFG1V5FmdmijbnPld{B1cGViYX70bZBzd2yrZnXyZZRqfmViZX\m[YN1KG:oIHXycI91cW6rYh?= NWn3XZpMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2O|U1QDRpPkK2Olc2PDh2PD;hQi=>
A549  MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXv1OoNyOTBxMUWvNlAhdk1? M3rsUFczyqCq MYHEUXNQ MoPt[Y5p[W6lZYOgeIhmKGGwdHnwdo9tcW[ncnH0bZZmKGWoZnXjeEBw\iCncnzveIlvcWJ? NIqwVYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[3OVQ5PCd-Mk[2O|U1QDR:L3G+
NCI-H460  MlXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;qbZlvOTBxMkCvN|Ahdk1? MUi3NuKhcA>? MUTEUXNQ NGfUdndmdmijbnPld{B1cGViYX70bZBzd2yrZnXyZZRqfmViZX\m[YN1KG:oIHXycI91cW6rYh?= NXL0NmVURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2O|U1QDRpPkK2Olc2PDh2PD;hQi=>
J89GFP Mn70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXTSG1UV8Li MXfFR|UxRTR7Lki1JOKyKDF{Lk[1JI5O M4\hTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NU[zOVY5Lz5{NkW2N|U3QDxxYU6=
THP89GFP MojSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPLSG1UV8Li NFG3R5JGSzVyPUG5MlM1KMLzIE[uOFMhdk1? MnrGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkO1OlgoRjJ4NU[zOVY5RC:jPh?=
SK-NEP-1 Mnq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4L3dFAvODIkgKOxNE4xKM7:TR?= MYmyOEBp MnPGSG1UV8Li MULJR|UxRTd4LkO0JI5O NEPk[Y89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
G401 NX2zZ41mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mli1NE4xOeLCk{GwMlAh|ryP M{m2TlI1KGh? NGj2dG9FVVORwrC= MWLJR|UxRTF2Mz6wNkBvVQ>? M3fYdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
SK-NEP-1 MoD5R4VtdCCYaXHibYxqfHliQYPzZZk> Mn60OVAhdk1? Mkm1NgKBmzRiZB?= MlP1SG1UV8Li MYDy[YR2[2W|IHPlcIwhe3W{dnn2ZYwhcW5iYTD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 MknjR4VtdCCYaXHibYxqfHliQYPzZZk> MV21NEBvVQ>? M1nNTVHjiJN2IHS= M{fHNGROW00EoB?= NEf2fIFz\WS3Y3XzJINmdGxic4Xyeol3[WxiaX6gZUB1cW2nIHTldIVv\GWwdDDtZY5v\XJ? M3XCUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
SK-NEP-1 NE\zXoRCeG:ydH;zbZMhSXO|YYm= NV;nblNFPTBxMUCwJI5O MWSyOEBp NX3I[2ZYTE2VT9Mg MnnNbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 MVjBdI9xfG:|aYOgRZN{[Xl? MoXwOVAwOTByIH7N NX\XcZNrOjRiaB?= M3zqSWROW00EoB?= MnXubY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M3O5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
SK-NEP-1 NFnrVJRHfW6ldHnvckBCe3OjeR?= Mn3FOVAwOTByIH7N NFr3UIgzPCCq MUjEUXNQyqB? MVPzbI94eyC2aHWgbY5lfWO2aX;uJI9nKESQQTDmdoFodWWwdHH0bY9v MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 M3K2Z2Z2dmO2aX;uJGF{e2G7 MnzOOVAwOTByIH7N MlLhNlQhcA>? NX3jWZgxTE2VT9Mg NGnte3h{cG:5czD0bIUhcW6mdXP0bY9vKG:oIFTORUBnemGpbXXueIF1cW:w MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
SK-NEP-1 NILtfZpHfW6ldHnvckBCe3OjeR?= NHT3RWw2OC9zMECgcm0> MmPSNlQhcA>? MUfEUXNQyqB? MXPpcoR2[2W|IHPlcIwh[3mlbHWg[Il{d3KmZYNCpC=> NYDZZlFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 NVvBPJkyTnWwY4Tpc44hSXO|YYm= M2POU|UxNzFyMDDuUS=> M2PybVI1KGh? NXvjZ2V5TE2VT9Mg MnTEbY5lfWOnczDj[YxtKGO7Y3zlJIRqe2:{ZHXyxsA> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
RPMI 8226 MnOwR4VtdCCVdYL2bZZidCCDc4PhfS=> NXfufIxPOi92L{[gcm0> NYH5c2xTPDkkgJno NF;hNnpqdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq NWjke25nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
OPM2 MoHJR4VtdCCVdYL2bZZidCCDc4PhfS=> NX3lemg1Oi92L{[gcm0> MXS0PQKBkWh? NIDo[49qdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq NWL1Omw2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
U266 NH:2fmZE\WyuIGP1dpZqfmGuIFHzd4F6 MUGyM|QwPiCwTR?= MVG0PQKBkWh? M3O1VIlv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? NUjLUlA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
H929 MWPD[YxtKFO3co\peoFtKEG|c3H5 MWiyM|QwPiCwTR?= M{XLVFQ56oDLaB?= M{XBVYlv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
RPMI 8226  Mor6RZBweHSxc3nzJGF{e2G7 NVrtTZVFPOLCiX7N NFr3O3UzPC92ODDo M2j5N4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NXG2d4RkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
HCC827 M{PxSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXOxNEBvVQ>? M3;Cc|Q5KGh? NXH1fYVSTE2VTx?= M3q2fIVvcGGwY3XzJINqe3CuYYTpckB{\W6|aYTpeol1gcLi M3q1VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUS0OlE4Lz5{NUm0OFYyPzxxYU6=
NCI-H23 NVjRVWI3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIf1bpoyOCCwTR?= NV:4RnA{PDhiaB?= M2TGTWROW09? Mm\i[Y5p[W6lZYOgZ4l{eGyjdHnuJJNmdnOrdHn2bZR6yqB? NWjxN2tYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OFQ3OTdpPkK1PVQ1PjF5PD;hQi=>
AML3 MljySpVv[3Srb36gRZN{[Xl? NGiycW0xNTFizszN M4jucFI1yqCq M2D0d4lv\HWlZYOgSG5CKG[{YXft[Y51[XSrb39CpIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NG\tTWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[xNlk1OSd-MkW2NVI6PDF:L3G+
ML-1 NIDRc3VHfW6ldHnvckBCe3OjeR?= M1HKVlAuOSEQvF2= MnzFNlTDqGh? MmKybY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NX;DRZl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
RPMI-8226vr10  Mkf1SpVv[3Srb36gRZN{[Xl? MoHhNE0yKM7:TR?= MnnnNlTDqGh? NHrOTm5qdmS3Y3XzJGRPSSCocnHncYVvfGG2aX;uxsBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NXK1[I5ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
ML-1 M{DVU2Z2dmO2aX;uJGF{e2G7 MX6xJO69VQ>? Mln3NlTDqGh? M4PZRolv[3KnYYPld{Bk[XOyYYPlMVMh[WO2aY\peJkhPC2ob3zk M17oc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
RPMI-8226vr10  MWDGeY5kfGmxbjDBd5NigQ>? MWixJO69VQ>? M{i1[VI1yqCq MlLzbY5kemWjc3XzJINie3Cjc3WtN{Bi[3Srdnn0fUAzNjVvZn;s[C=> NYPHTm9TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
SK-N-BE (2) MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33VZVI16oDLaB?= NHvTRY5KSzVyPUGwOE4x6oDLwsJihKk4Njhibl2= MmDVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-BE (2), PAN  MK M1\lT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjheWdYOjUkgJno MX\JR|UxRTFyND6w5qCKyrIkgJm3Mlghdk1? MlzaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-BE (2), MK  PAN NX;SToVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\memYzPOLCiXi= NITqUnRKSzVyPUO4Nk4x6oDLwsJihKk1Oy5{IH7N MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV|MEi5NVY9N2F-
SK-N-AS M3noRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmD1NlTjiImq NY[xS|d3UUN3ME2zO{4y6oDLwsJihKkzNjRibl2= NHLiNYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-DZ NUi5enQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUKyOQKBkWh? NG\qbHhKSzVyPUG3MlHjiIoEsfMAjVAvPCCwTR?= NXrTPZhRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N|A5QTF4PD;hQi=>
Caki-1 NX61RVhrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjjUGEyOC9{NT:1NEBvVQ>? NIXESZI1QCCq MYXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldkB{gW6ncnfpd5Rq[2GubImge4l1cCC{aYTvcoF3cXJ? NF;kbFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PVE6OSd-MkWyO|kyQTF:L3G+
ACHN NFrVT4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrNNVAwOjVxNUCgcm0> Mk\5OFghcA>? MX3pcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldkB{gW6ncnfpd5Rq[2GubImge4l1cCC{aYTvcoF3cXJ? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5OUG5NUc,OjV{N{mxPVE9N2F-
769-P NXvzSWVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYqxNE8zPS93MDDuUS=> NULsdJZNPDhiaB?= MXTpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldkB{gW6ncnfpd5Rq[2GubImge4l1cCC{aYTvcoF3cXJ? Mkm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{mxPVEoRjJ3Mke5NVkyRC:jPh?=
786-O  M4\FWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTZNVAwOjVxNUCgcm0> MVG0PEBp NHP4PW1qdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meiC|eX7ldodqe3SrY3HscJkhf2m2aDDybZRwdmG4aYK= NEDH[nU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PVE6OSd-MkWyO|kyQTF:L3G+
Caki-1 M3\6XGFxd3C2b4Ppd{BCe3OjeR?= MoC2OVAhdk1? M2myUFQ5KGh? M2HuV4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3;tZolv\WRicnn0c45ifmm{ Mny4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{mxPVEoRjJ3Mke5NVkyRC:jPh?=
ACHN NIjQbVVCeG:ydH;zbZMhSXO|YYm= MkXvOVAhdk1? NYLENlZjPDhiaB?= M4n1W4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3;tZolv\WRicnn0c45ifmm{ NHnVWmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PVE6OSd-MkWyO|kyQTF:L3G+
769-P MmfTRZBweHSxc3nzJGF{e2G7 MorBOVAhdk1? MVe0PEBp M2rlc4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3;tZolv\WRicnn0c45ifmm{ NWnmbmd1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO|kyQTFpPkK1Nlc6OTlzPD;hQi=>
786-O  MXfBdI9xfG:|aYOgRZN{[Xl? NWj6V|loPTBibl2= NFH4c4c1QCCq NV34RZRPcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDjc41jcW6nZDDybZRwdmG4aYK= NUXlcIsxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO|kyQTFpPkK1Nlc6OTlzPD;hQi=>
Caki-1 NInEe|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX2yOU82OCCwTR?= NVvrZXRYPDhiaB?= MmHPSG1UVw>? NYnzU2xGcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJic4nu[ZJocXO2aXPhcIx6KHerdHigZo9zfGW8b33pZi=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
ACHN NFrvZYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzsNlUwPTBibl2= NHnIV5I1QCCq NELld4VFVVOR MlHVbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZIhe3mwZYLnbZN1cWOjbHz5JJdqfGhiYn;yeIV7d22rYh?= NH\sTGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG3OlM2PCd-MkWxO|Y{PTR:L3G+
769-P MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TKOlI2NzVyIH7N MUe0PEBp NIfF[YhFVVOR MojDbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZIhe3mwZYLnbZN1cWOjbHz5JJdqfGhiYn;yeIV7d22rYh?= M1roSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUe2N|U1Lz5{NUG3OlM2PDxxYU6=
Caki-1 NF;ufVVEd2yxbomgSo9zdWG2aX;uJGF{e2G7 NWXpXVBxPTBibl2= MoH4O{0yPCCm M{DWN2ROW09? MYjzeZBxemW|c3XkJINwdG:weTDmc5Ju[XSrb36gd4lodmmoaXPhcpRtgSClb33ibY5m\CC5aYToJJdqfGhiYn;yeIV7d22rYjFCpC=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
ACHN MmXlR49td267IF\vdo1ifGmxbjDBd5NigQ>? MkiyOVAhdk1? M3X2fFcuOTRiZB?= NFOzSFFFVVOR Mofxd5VxeHKnc4Pl[EBkd2yxbomg[o9zdWG2aX;uJJNq\26rZnnjZY51dHliY3;tZolv\WRid3n0bEB4cXSqIHLvdpRmgm:vaXKgxsA> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
769-P M3XmU2NwdG:weTDGc5Ju[XSrb36gRZN{[Xl? MVy1NEBvVQ>? M1zNV|cuOTRiZB?= NHe2d4hFVVOR NHrjTHl{fXCycnXzd4VlKGOxbH;ufUBnd3KvYYTpc44he2mpbnnmbYNidnSueTDjc41jcW6nZDD3bZRpKHerdHigZo9zfGW8b33pZkDDqA>? Mlf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzN{[zOVQoRjJ3MUe2N|U1RC:jPh?=
Caki-1 M4j2TWFxd3C2b4Ppd{BCe3OjeR?= NGe4RnY2OCCwTR?= NGK3U|k1QCCq MWnEUXNQ M2HZOYlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
ACHN MoHsRZBweHSxc3nzJGF{e2G7 M2TpXVUxKG6P NHX0R|Y1QCCq NWjnWHdtTE2VTx?= NIPydZlqdmS3Y3XzJINmdGxiYYDvdJRwe2m| MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5NkO1OEc,OjVzN{[zOVQ9N2F-
769-P NE\OXXlCeG:ydH;zbZMhSXO|YYm= MVy1NEBvVQ>? MXK0PEBp M{T2U2ROW09? NXz1VpUzcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NFzpNYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG3OlM2PCd-MkWxO|Y{PTR:L3G+
MDA-MB-231 MVvNc5JxcG:ub3fpZ4FtKEO{eYP0ZYwhXmmxbHX0JEhEXiliQYPzZZk> M4HKRVExyqCwTR?= MV[zxsBl Mn;ySG1UVw>? MWDhcJRmenNiY3XscEBud3KyaH;sc4d6yqB? NELURnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixNFQ6Pyd-MkS4NVA1QTd:L3G+
BT-549 MXLNc5JxcG:ub3fpZ4FtKEO{eYP0ZYwhXmmxbHX0JEhEXiliQYPzZZk> Mnj5NVDDqG6P M2nsblPDqGR? NFP0fWhFVVOR NYGxS2VY[Wy2ZYLzJINmdGxibX;ydIhwdG:pedMg NIfCfWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixNFQ6Pyd-MkS4NVA1QTd:L3G+
MCF-7  NV;wdJkyVW:{cHjvcI9ocWOjbDDDdpl{fGGuIG\pc4xmfCBqQ2[pJGF{e2G7 MUGxNOKhdk1? NEDxT|U{yqCm M2LuWWROW09? NEPUXnVidHSncoOgZ4VtdCCvb4LwbI9td2e7wrC= MmDYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUC0PVcoRjJ2OEGwOFk4RC:jPh?=
MCF-7 MojISpVv[3Srb36gRZN{[Xl? NFLBUlE2NTVyIH7N MkO3NlQhcA>? MX3EUXNQ NULnTHZKemWmdXPl[EB1cGVibHX2[Ywhd2ZiZYjwdoV{e2mxbjDv[kBGWs7zLDDQVkBidmRiRn;4RVHDqA>? M4PEU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[2OFA4Lz5{NEO2OlQxPzxxYU6=
CTS M{LmNmFxd3C2b4Ppd{BCe3OjeR?= M3jJV|DjiJN2MDDuUeKh NVLveY5jPDhiaB?= M3zOO4lv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NYCwem9WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQ1OjlpPkK0NlQ1PDJ7PD;hQi=>
OCI-AML3  M2n3Z2Fxd3C2b4Ppd{BCe3OjeR?= M1vGclDjiJN2MDDuUeKh M4nLfFQ5KGh? MYDpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NESyPUc,OjR{NES0Nlk9N2F-
U937 Mk\LRZBweHSxc3nzJGF{e2G7 NI\JN48x6oDVNECgcm3DqA>? M{PUUFQ5KGh? M2jLWIlv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NESyPUc,OjR{NES0Nlk9N2F-
PC3 Mm\3RZBweHSxc3nzJGF{e2G7 M{flW|AuOTByIH7N NH7HSpczPC92ODDo MoTCbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4M{KzNEc,OjRzNkOyN|A9N2F-
PC3-AR NE[zTG1CeG:ydH;zbZMhSXO|YYm= MmfKNE0yODBibl2= MnnYNlQwPDhiaB?= NHX4SJpqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NU\JbYc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlMzOzBpPkK0NVY{OjNyPD;hQi=>
PC3 NXLDd5ZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDqNE0yODBibl2= MX2yOE81QCCq MYnpcoR2[2W|IHHjZ5VufWyjdHnvckBw\iC|dXLHNUBxd3C3bHH0bY9v NVHndHRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlMzOzBpPkK0NVY{OjNyPD;hQi=>
PC3-AR NU[zSVN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnkeGtEOC1zMECgcm0> M1y1SlI1NzR6IHi= M4PaRYlv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgbY4hfGinIFeyUUBxcGG|ZR?= NHntNow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG2N|I{OCd-MkSxOlMzOzB:L3G+
PC3 NGHFW3dHfW6ldHnvckBCe3OjeR?= MWewMVExOCCwTR?= NIH4SWozPCCq NF;KR4x{fXCycnXzd4V{KGW6cILld5Nqd25ib3[gZYN1cX[jdHXkJGFVVSxiQXv0JIFv\CCHcnuxM|IheHKxdHXpci=> M1\rWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[zNlMxLz5{NEG2N|I{ODxxYU6=
PC3-AR MnH3SpVv[3Srb36gRZN{[Xl? MVewMVExOCCwTR?= NXLzbodwOjRiaB?= NVLxbldje3WycILld5NmeyCneIDy[ZN{cW:wIH;mJIFkfGm4YYTl[EBCXE1uIFHreEBidmRiRYLrNU8zKHC{b4TlbY4> NFTQOZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG2N|I{OCd-MkSxOlMzOzB:L3G+
OS-RC-2 NVrr[nZmS2WubDDWbYFjcWyrdImgRZN{[Xl? NY\ke3h7OC1zMECwJI5O Mny0NlQwPDhxN{KgbC=> MkezSG1UVw>? M1:1e4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M4HzUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS0O|M4Lz5{NEG0OFc{PzxxYU6=
OS-RC-2 MlHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HxVVUxKG6P M2LRNVQ5KGh? M4fxd2ROW09? M2XuNolv\HWlZYOgS|IwVSCjcoLld5Q> NIqzVm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0OFc{Pyd-MkSxOFQ4Ozd:L3G+
OS-RC-2 MV3BdI9xfG:|aYOgRZN{[Xl? MXq1NEBvVQ>? M1O1VVQ5KGh? M1TqWmROW09? Ml\GbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NV21W|VuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFQ4OzdpPkK0NVQ1PzN5PD;hQi=>
SK-N-AS MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIezRpgx6oDVOECgcm0> M1TvNVQ5KGh? NGDVbXlKSzVyPUK3MlQhdk1? MmD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
SK-N-DZ NV3sWVBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofPNQKBmzhyIH7N Mmj3OFghcA>? M4DoW2lEPTB;MkGuPUBvVQ>? NV\Cb25FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg4QTlpPkK0NFk5Pzl7PD;hQi=>
SK-N-SH M{LXdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\0clAx6oDVOECgcm0> MUW0PEBp M2P5XGlEPTB;N{KuN{BvVQ>? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEe5PUc,OjRyOUi3PVk9N2F-
SK-N-BE NH3QXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXmw5qCUQDBibl2= MknhOFghcA>? MknJTWM2OD15NT60JI5O MnXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
SK-N-AS MUfBdI9xfG:|aYOgRZN{[Xl? NYHHWJFkOOLCk{iwJI5O NIfCdIc1QCCq MVTwc5RmdnSueTDpcoR2[2WmIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JIZie2irb36= MnP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
SK-N-DZ NWXGdoZrSXCxcITvd4l{KEG|c3H5 M{LiblDjiJN6MDDuUS=> NEDibG41QCCq MXnwc5RmdnSueTDpcoR2[2WmIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JIZie2irb36= NXvBU|FrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg4QTlpPkK0NFk5Pzl7PD;hQi=>
SK-N-SH NFvXWZNCeG:ydH;zbZMhSXO|YYm= Mkj6NQKBmzRyIH7N NVXvSoFYPDhiaB?= NVfa[HpveG:2ZX70cJkhcW6mdXPl[EBieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCoYYPobY9v MlzVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
SK-N-BE MYLBdI9xfG:|aYOgRZN{[Xl? M4L3XVDjiJN2MDDuUS=> NGHBeHg1QCCq NW\jUphpeG:2ZX70cJkhcW6mdXPl[EBieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCoYYPobY9v M1;hUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm4O|k6Lz5{NEC5PFc6QTxxYU6=
SK-N-AS M3:wSmZ2dmO2aX;uJGF{e2G7 NHqyZ2kx6oDVOECgcm0> M{XifVQ5KGh? M4ftbolv\HWlZYOgZUBld3OnLXTldIVv\GWwdDDjcIVifmGpZTDv[kBk[XOyYYPlJFMh[W6mIGDBVnA> MlTwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3PVkoRjJ2MEm4O|k6RC:jPh?=
SK-N-DZ NF\LSVJHfW6ldHnvckBCe3OjeR?= MoriNQKBmzhyIH7N Mo\6OFghcA>? MnrnbY5lfWOnczDhJIRwe2VvZHXw[Y5l\W62IHPs[YF3[WenIH;mJINie3Cjc3WgN{BidmRiUFHSVC=> NV\pUoVKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg4QTlpPkK0NFk5Pzl7PD;hQi=>
SK-N-SH MYXGeY5kfGmxbjDBd5NigQ>? M4LxclDjiJN2MDDuUS=> NVTFSpNGPDhiaB?= MV3pcoR2[2W|IHGg[I9{\S2mZYDlcoRmdnRiY3zlZZZi\2Vib3[gZ4F{eGG|ZTCzJIFv\CCSQWLQ NH7hdnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5PFc6QSd-MkSwPVg4QTl:L3G+
SK-N-BE MX7GeY5kfGmxbjDBd5NigQ>? MnHFNQKBmzRyIH7N MlnMOFghcA>? MkntbY5lfWOnczDhJIRwe2VvZHXw[Y5l\W62IHPs[YF3[WenIH;mJINie3Cjc3WgN{BidmRiUFHSVC=> M{PQdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm4O|k6Lz5{NEC5PFc6QTxxYU6=
HCC-LM3 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX[1fXJDOS1zMECwJI5O MViyOE81QC95MjDo MmH6SG1UVw>? NXXQdoNPcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NUfEVY1oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
HepG2 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NED6V5cyNTFyMECgcm0> NYDGTJh{OjRxNEivO|IhcA>? MmP2SG1UVw>? NYfjNJc3cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
SMMC-7721 MmD5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoK2NU0yODByIH7N MUSyOE81QC95MjDo M3TJO2ROW09? MXvpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
HCC-LM3 M3G3VmFxd3C2b4Ppd{BCe3OjeR?= MUe1NEBvVQ>? M{TCO|Q5KGh? NGfPW3dFVVOR M4LFRolv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3nncolncWOjboTsfUBqdiCjIHPhd5Bie2VvZHXw[Y5l\W62IH3hco5meiCkeTDjcIVifmGpZTDv[kBk[XOyYYPld{A{NCB6IHHu[EA6 M2rIUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
HepG2 M1LHPGFxd3C2b4Ppd{BCe3OjeR?= NHz4fm42OCCwTR?= MnfwOFghcA>? NGHofoZFVVOR NV\oPWpkcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzbYdvcW[rY3HueIx6KGmwIHGgZ4F{eGG|ZT3k[ZBmdmSnboSgcYFvdmW{IHL5JINt\WG4YXflJI9nKGOjc4Dhd4V{KDNuIEigZY5lKDl? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
SMMC-7721 NEm2SZdCeG:ydH;zbZMhSXO|YYm= NYW0ZlRrPTBibl2= NFnoW241QCCq Mn7VSG1UVw>? M2LKZolv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3nncolncWOjboTsfUBqdiCjIHPhd5Bie2VvZHXw[Y5l\W62IH3hco5meiCkeTDjcIVifmGpZTDv[kBk[XOyYYPld{A{NCB6IHHu[EA6 NFzifVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3 NGHINGVHfW6ldHnvckBCe3OjeR?= NUHSUYdKPTBxMUCwJI5O M2jUN|I1KGh? MknsSG1UVw>? MWPk[YNz\WG|ZYOgeIhmKGyndnXsd{Bw\iCyLWPURXQ{KGGwZDDwMWFsfMLi NHvQNZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HepG2 NELib2tHfW6ldHnvckBCe3OjeR?= NGn3NI82OC9zMECgcm0> MVKyOEBp M13WV2ROW09? NIjIcFNl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDwMXNVSVR|IHHu[EBxNUGtdNMg MnHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 Ml7xSpVv[3Srb36gRZN{[Xl? M1XnOFUxNzFyMDDuUS=> NXvXXXBpOjRiaB?= M3\2WGROW09? MoLQ[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicD3TWGFVOyCjbnSgdE1Cc3UEoB?= M4XGdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
HCC-LM3 MnfFSpVv[3Srb36gRZN{[Xl? MnLzOVAwOTByIH7N NY\mU5VYOjRiaB?= MnT5SG1UVw>? MUDkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
HepG2 NEjPdnBHfW6ldHnvckBCe3OjeR?= MoTNOVAwOTByIH7N MmfqNlQhcA>? M4HPSGROW09? NGrS[mlld3ewcnXneYxifGW|IFLjcE15VCCneIDy[ZN{cW:w M{LBfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
SMMC-7721 M4rlXWZ2dmO2aX;uJGF{e2G7 MWW1NE8yODBibl2= NGKwbHczPCCq NXnrW29MTE2VTx?= M3fQN4Rwf26{ZXf1cIF1\XNiQnPsMZhNKGW6cILld5Nqd25? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7M{m1Okc,OjRyOUO5OVY9N2F-
FaDu Mm[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXKxNFDjiIWwTR?= MnvzPE8yOC9zMjDo Mnew[Il{eGyjeXXkJIEhe2mpbnnmbYNidnRiYX7kJJBzd2yxbnfl[EBIOi:PIHHydoV{fCCjdDC4JIFv\CBzMvMAiYgheG:|dDDy[Yxm[XOn M4PUVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK2OFgzLz5{NECyOlQ5OjxxYU6=
FaDu NYrJNGZYTnWwY4Tpc44hSXO|YYm= M1PCVlExOOLChX7N NIWyTpYzNzRxOD:xNkBp NUP2Vow3cW6mdXPl[EBxOjGZYX[xM2NqeDIEoHX4dJJme3Orb36= NXXWS4ZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlY1QDJpPkK0NFI3PDh{PD;hQi=>
PC-3  M{HaPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2i1eVAuOTBizszN NXrLeVhCOjRxNEivO|IhcA>? NV34ZoN7cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M2nzdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUmxNlE3Lz5{M{m5NVIyPjxxYU6=
LNCaP NHOxU2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M173c|AuPSEQvF2= NEOzN4QzPC92OD:3NkBp NXyxNGI3cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NWDa[oFGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PVEzOTZpPkKzPVkyOjF4PD;hQi=>
RWPE-1  MnzzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zOclAuOjBizszN MkL1NlQwPDhxN{KgbC=> Mmn5bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MoX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7OUGyNVYoRjJ|OUmxNlE3RC:jPh?=
Capan-1 NF7mR4dHfW6ldHnvckBCe3OjeR?= NGrwfYMzPS93MD:xNFAhdk1? MoPNPE8zPC92ODDo NVLnRmh4TE2VTx?= NXnGUYpj\G:5boLl[5Vt[XSnZDDSc44hdVKQQTDhcoQheHKxdHXpckBmgHC{ZYPzbY9vKGGwZDDkc5dve3S{ZXHtJJNq\26jbHnu[y=> MlfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ|OUKyPFg3RC:jPh?=
L3.6pl NGjtZ|ZHfW6ldHnvckBCe3OjeR?= NWDmbFZ3OjVxNUCvNVAxKG6P NEDoXHQ5NzJ2L{S4JIg> NHjjRYpFVVOR NFz1NWZld3ewcnXneYxifGWmIGLvckBuWk6DIHHu[EBxem:2ZXnuJIV5eHKnc4Ppc44h[W6mIHTve45{fHKnYX2gd4lodmGuaX7n NVz3Z|IxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
CFPAC-1  NGXDcoxHfW6ldHnvckBCe3OjeR?= NX;meWJjOjVxNUCvNVAxKG6P MkTwPE8zPC92ODDo NW\sRphYTE2VTx?= MW\kc5dvemWpdXzheIVlKFKxbjDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36gZY5lKGSxd37zeJJm[W1ic3nncoFtcW6p M3TaRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUKyPFg3Lz5{M{myNlg5PjxxYU6=
Capan-1 MmmwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXmyOU82OC9zMECgcm0> MmPrOFghcA>? MkHwSG1UVw>? NHvDeXhz\WS3Y3XzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl{Mki4Okc,OjN7MkK4PFY9N2F-
L3.6pl MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXneYxyOjVxNUCvNVAxKG6P M3uxT|Q5KGh? NYTkenNZTE2VTx?= M2\xepJm\HWlZYOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1fsUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUKyPFg3Lz5{M{myNlg5PjxxYU6=
CFPAC-1  Mn3mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWCyOU82OC9zMECgcm0> NH3zSXU1QCCq NGP0RXBFVVOR NE[5NnRz\WS3Y3XzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl{Mki4Okc,OjN7MkK4PFY9N2F-
Capan-1 MUjBdI9xfG:|aYOgRZN{[Xl? NVzlenpXOjVxNUCvNVAxKG6P M3v0OFQ5KGh? NE\jeWNFVVOR MkX3bY5lfWOnczDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M4nLfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUKyPFg3Lz5{M{myNlg5PjxxYU6=
L3.6pl M3TPbGFxd3C2b4Ppd{BCe3OjeR?= MnLKNlUwPTBxMUCwJI5O MYG0PEBp NFfKPVFFVVOR MYTpcoR2[2W|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NFvUPZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{myNlg5Pid-MkO5NlI5QDZ:L3G+
CFPAC-1  MXfBdI9xfG:|aYOgRZN{[Xl? NH7qcmIzPS93MD:xNFAhdk1? NVHvdnA{PDhiaB?= NVvGVmVsTE2VTx?= Mn7DbY5lfWOnczDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NYe3V4NRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
HN22 Ml:zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGP6VIgxNTJyIH7N MnuxNlQwPDhiaB?= NH\uSmJFVVOR NGfJNHBqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIITpcYUuKGGwZDDkc5NmNSCmZYDlcoRmdnRibXHucoVz NYjtWXJVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|czOzVpPkKzPFc4OjN3PD;hQi=>
HSC4  MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2m1flAuOjBibl2= NVX5S5dnOjRxNEigbC=> NIXsT5RFVVOR NGrUVIpqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBjd3SqIITpcYUuKGGwZDDkc5NmNSCmZYDlcoRmdnRibXHucoVz NYP5cYxqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|czOzVpPkKzPFc4OjN3PD;hQi=>
HN22 NX;NcHk2SXCxcITvd4l{KEG|c3H5 NX7NVHhiOC1{MDDuUS=> M{DGdlQ5KGh? MVHEUXNQ M4rPbYlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NFLJN2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3O|I{PSd-MkO4O|czOzV:L3G+
HSC4  M3TQe2Fxd3C2b4Ppd{BCe3OjeR?= M36zXVAuOjBibl2= MmTYOFghcA>? Mkj0SG1UVw>? NX\zRmRrcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HN22 NFrReFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWiwMVIxKG6P NFn1c2c1QCCq Mm\1SG1UVw>? MWnpcoR2[2W|IFexJJBp[XOnIHPlcIwh[3mlbHWgZZJz\XO2wrC= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HSC4  NH7K[nVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVqwMVIxKG6P MoTMOFghcA>? NYXxPXB4TE2VTx?= MX;pcoR2[2W|IFexJJBp[XOnIHPlcIwh[3mlbHWgZZJz\XO2wrC= MlnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{eyN|UoRjJ|OEe3NlM2RC:jPh?=
HN22 MXTGeY5kfGmxbjDBd5NigQ>? NHKw[IIxNTJyIH7N M{DXdlQ5KGh? M1TxcmROW09? MXLzeZBxemW|c3XzJHNxOSCneIDy[ZN{cW:wwrC= MnPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{eyN|UoRjJ|OEe3NlM2RC:jPh?=
HSC4  MWHGeY5kfGmxbjDBd5NigQ>? MoGyNE0zOCCwTR?= NEjlR5I1QCCq MnPxSG1UVw>? M{DnNZN2eHC{ZYPz[ZMhW3BzIHX4dJJme3Orb39CpC=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
Cal62 MljIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk[5TWM2OD1|MzFCtUA1KG6P M4rkdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK0NFY1Lz5{M{iyOFA3PDxxYU6=
Hth7 NEfYW3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDCTWM2OD1zNTFCtUAzKG6P MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC2OEc,OjN6MkSwOlQ9N2F-
Hth83 Mle4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUG5OnoxUUN3ME2zOEDDuSB3IH7N NEnYOJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOFA3PCd-MkO4NlQxPjR:L3G+
C643 Mmj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7pT5VvUUN3ME23NUDDuSBzMDDuUS=> NW\M[WpSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlQxPjRpPkKzPFI1ODZ2PD;hQi=>
SW1736 NHLNfYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPrTWM2OD1|NTFCtUA5KG6P MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC2OEc,OjN6MkSwOlQ9N2F-
T241 M1\YO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG[xVZBKSzVyPU[1JOKyKDdibl2= MoSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkSwOlQoRjJ|OEK0NFY1RC:jPh?=
T351 M1\uO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzlTpZKSzVyPUWwJOKyKDFyIH7N NHXiZ4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOFA3PCd-MkO4NlQxPjR:L3G+
BHP2-7 NUe0eZdST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zOXGlEPTB;M{egxtEhPiCwTR?= M2r0WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK0NFY1Lz5{M{iyOFA3PDxxYU6=
T238 MnLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTFuNUCwJOKyKDJyMDDuUS=> MnraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkSwOlQoRjJ|OEK0NFY1RC:jPh?=
HCT8 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXkO|IhcA>? NEPyOYRFVVOR NWOxdZZ2UUN3ME2xNk466oDLwsJihKkyNjlibl2= M4P2U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mkm5N|g5Lz5{M{K5PVM5QDxxYU6=
H630 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETyOI44OiCq NUjGdYdJTE2VTx?= NWjoPFJ{UUN3ME2xNk416oDLwsJihKk{NjFibl2= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7OUO4PEc,OjN{OUmzPFg9N2F-
cH630 5-FU-res MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYGydm13PzJiaB?= NVXJcYgzTE2VTx?= NFvDeJBKSzVyPUG1MlXjiIoEsfMAjVEvOiCwTR?= MlzMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUmzPFgoRjJ|Mkm5N|g5RC:jPh?=
HCT116 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXG3NkBp MW\EUXNQ MXvJR|UxRTFyLkhihKnDueLCiUKuNkBvVQ>? NIfSOJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
HCT116 p53−/− MnSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\ZN|I4OiCq M1i0dWROW09? MkHCTWM2OD16LkdihKnDueLCiUGuO{BvVQ>? NHf5TmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
dHCT116 p21−/− MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vwc|czKGh? NGrlNmVFVVOR M1X6TGlEPTB;NT655qCKyrIkgJmxMlMhdk1? NYiyOItZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPVk{QDhpPkKzNlk6Ozh6PD;hQi=>
HT29 NHHkdoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfqO|IhcA>? NH7OV2dFVVOR NWTLfFY{UUN3ME2xOk4{6oDLwsJihKkzNjNibl2= NFTBRnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
LoVo MoTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3:2NFczKGh? MXfEUXNQ NFfJdZBKSzVyPUWuNgKBkcLz4pEJNE43KG6P NHGyV449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5PVM5QCd-MkOyPVk{QDh:L3G+
RKO NUX1cphXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfnd204OiCq MUTEUXNQ MVzJR|UxRTdwOfMAjeKy6oDLMj6yJI5O MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7OUO4PEc,OjN{OUmzPFg9N2F-
SW480 NHXjOWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU[3NkBp NVflXpJnTE2VTx?= NXv1dY9SUUN3ME2xO{426oDLwsJihKkxNjhibl2= MlP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUmzPFgoRjJ|Mkm5N|g5RC:jPh?=
eSW620 NWT0d|R1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjaO|IhcA>? NWC1e5RpTE2VTx?= NGLTU3dKSzVyPUmuNgKBkcLz4pEJNk4yKG6P MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7OUO4PEc,OjN{OUmzPFg9N2F-
S2 NGqxXZFHfW6ldHnvckBie3OjeR?= NU\OU2pLUW6qaXLpeIlwdiCxZjDQcIF{dW:maYXtJIZidGOrcHHyeY0hUESDQ{Gg[ZhxemW|c3XkJIlvKES{b4PvdIhqdGFibXXsZY5w\2G|dHXyJHMzKGOnbHzzMEBKSzVyIE2gNE4xODF6IN88UU4> NELiVoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOxO|Q2OCd-MUmzNVc1PTB:L3G+
COLO205 NXTiW2dGSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NWrkdXBiQTZiaILz NWDYOo5MSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDU2xQOjB3IHPlcIx{KGGodHXyJFk3KGi{czDifUBk\WyudHn0[ZIhQTZiYYPzZZktKEmFNUCgQUAxNjBzODFOwG0v M13nZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkO0OFMxLz5{MU[zOFQ{ODxxYU6=
PC3 NF7qT4tCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MoPjPVYhcHK| NFzyPVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDh[pRmeiB7NjDodpMh[nliY3XscJRqfGW{IEm2JIF{e2G7LDDJR|UxKD1iMD6wNlQh|ryPLh?= NX;ZT3N[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2N|Q1OzBpPkKxOlM1PDNyPD;hQi=>
A2780 NXn5VJBtSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1;sbFk3KGi{cx?= NUHQbnQ2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC5OkBpenNiYomgZ4VtdHSrdHXyJFk3KGG|c3H5MEBKSzVyIE2gNE4xOzVizszNMi=> NXq5Rm8xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2N|Q1OzBpPkKxOlM1PDNyPD;hQi=>
HCT116 M2\PRWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnewPVYhcHK| M3fTcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDj[YxtfGm2ZYKgPVYh[XO|YYmsJGlEPTBiPTCwMlA1QCEQvF2u MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ|NESzNEc,OjF4M{S0N|A9N2F-
HEK293 NUDPbpVuTnWwY4Tpc44h[XO|YYm= NHe1OXNKdmirYnn0bY9vKG:oIFjERWM{KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{B2e2mwZzDbN2he[WOndInsZZRm\CCqdX3hckBpcXO2b37lJGg1KHCncITp[IUh[XNic4Xid5Rz[XSnIHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFnDOVAhRSByLkCwNlEh|ryPLh?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2NEewNUc,OjJ|NES3NFE9N2F-
HEK293 NXPiSJZ6TnWwY4Tpc44h[XO|YYm= MVjJcohq[mm2aX;uJI9nKEiGQVOxJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczD1d4lv\yCdM1jdZYNmfHmuYYTl[EBpfW2jbjDobZN1d26nIFi0JJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUCgQUAxNjByMkWg{txONg>? NFy3dXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO0OFcxOSd-MkKzOFQ4ODF:L3G+
HEK293 NGK4TlRHfW6ldHnvckBie3OjeR?= MYrJcohq[mm2aX;uJI9nKEiGQVO2JEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczD1d4lv\yCdM1jdZYNmfHmuYYTl[EBpfW2jbjDobZN1d26nIFi0JJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUCgQUAxNjBzMTFOwG0v MmT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NES3NFEoRjJ{M{S0O|AyRC:jPh?=
SF21 NGHYXlZHfW6ldHnvckBie3OjeR?= M17GNWlvcGmkaYTpc44hd2ZiZnzh[{11[WepZXSgTGRCSzJiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gV2YzOSClZXzsd{B2e2mwZzDbN2he[WOndInsZZRm\CCqdX3hckBpcXO2b37lJGg1KHCncITp[IUh[XNic4Xid5Rz[XSnIHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFnDOVAhRSByLkCxN{DPxE1w M1r3ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{S0O|AyLz5{MkO0OFcxOTxxYU6=
HEK293 MUPGeY5kfGmxbjDhd5NigQ>? MkmxTY5pcWKrdHnvckBw\iCKRFHDOEApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgeZNqdmdiW{PIYYFk\XS7bHH0[YQhcHWvYX6gbIl{fG:wZTDIOEBx\XC2aXTlJIF{KHO3YoP0doF1\SCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCLQ{WwJF0hOC5{IN88UU4> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2NEewNUc,OjJ|NES3NFE9N2F-
SF9 NGnPdZVHfW6ldHnvckBie3OjeR?= NGS4WFhKdmirYnn0bY9vKG:oIHjpd{1{fHKncD30ZYdo\WRiSFTBR|ghMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iU1[5JINmdGy|IIXzbY5oKFt|SG3hZ4V1gWyjdHXkJIh2dWGwIHjpd5RwdmViSESgdIVxfGmmZTDhd{B{fWK|dILheIUh[nlic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whUUN3MDC9JFAvOjhizszNMi=> MkmyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NES3NFEoRjJ{M{S0O|AyRC:jPh?=
B16 M{HRR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NU\DdIxWPDhiaILz M1z5N2dzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJGIyPiClZXzsd{BqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGdKPTBiPTCwMlE2KM7:TT6= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzByOUKwN{c,OjNyMEmyNFM9N2F-
HeLa NXu1So04TnWwY4Tpc44h[XO|YYm= NWXVOJZuUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHGgZ4VtdHNidYPpcochTmy3b4Kg[IUhVHm|IHHzJJN2[nO2cnH0[UBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCzJO69VS5? NUDwcnJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2N|k2OzdpPkKzOlM6PTN5PD;hQi=>
HuH7 Mmr6R5l1d3SxeHnjbZR6KGG|c3H5 MVSzJIRigXN? MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCC4aXHibYxqfHliYX\0[ZIhOyCmYYnzJIJ6KEOnbHzUbZRmeiB7NjDhd5NigSxiQ1O1NEA:KDBwMECzOUDPxE1w NHTBZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NFcxOCd-MkW0PVA4ODB:L3G+
Sf9 NUfGc5RYTnWwY4Tpc44h[XO|YYm= NG\xfoQyPSCvaX7z NHvMN3lKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJGMufGW{bXnuZYwhUGm|L1\MRWcufGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JGhFSUNzIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCrboPlZ5QhW2Z7IHPlcIx{KHW|aX7nJGFkNXCncITp[IUuSU2FIHHzJJN2[nO2cnH0[UBie3Onc4Pl[EBieyC{ZXzlZZNmKG:oIFHNR{BxemWrbnP1ZoF1\WRiZn;yJFE2KG2rboOg[o9tdG:5ZXSgZpkhe3Wkc4TyZZRmKGGmZHn0bY9vKG2nYYP1doVlKGFuIFnDOVAhRSByLkCwNVI3KM7:TT6= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O|Y9N2F-
Sf9 NGjDXIxHfW6ldHnvckBie3OjeR?= NEDjO3IyPSCvaX7z MmPiTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDDMZRmem2rbnHsJGhqey22YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQhUESDQ{OvUmNQWjJiKEO5OUB1dyB2OEmgdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDpcpNm[3RiU3[5JINmdGy|IIXzbY5oKEGlLYDldJRq\GVvQV3DJIF{KHO3YoP0doF1\SCjc4Pld5Nm\CCjczDy[Yxm[XOnIH;mJGFOSyCycnXpcoN2[mG2ZXSg[o9zKDF3IH3pcpMh\m:ubH;3[YQh[nlic4Xid5RzNCCLQ{WwJF0hOC5yMEKyO{DPxE1w NG\lfVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
MV4-11 NV3M[JhHS3m2b4TvfIlkcXS7IHHzd4F6 NUXjfVV2OjRiaILz NHWzcXVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFAzQTdizszNMi=> Ml3ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O|YoRjJ5MUi2Olc3RC:jPh?=
Sf9 MlHNSpVv[3Srb36gZZN{[Xl? NXvSZ2x4OTVibXnudy=> Ml:2TY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOiCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6|ZXP0JHNnQSClZXzsd{B2e2mwZzDBZ{1x\XC2aXTlMWFOSyCjczDzeYJ{fHKjdHWgZZN{\XO|ZXSgZZMhemWuZXHz[UBw\iCDTVOgdJJmcW6ldXLheIVlKG[xcjCxOUBucW6|IH\vcIxwf2WmIHL5JJN2[nO2cnH0[UBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjCxJIhzKGK7IH\seY9z\XOlZX7j[UBiNCCLQ{WwJF0hOC5yMEOyPEDPxE1w MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O|Y9N2F-
HCT116 MoKwR5l1d3SxeHnjbZR6KGG|c3H5 M4GzcFI1KGi{cx?= NHjCSo9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFA{OzZizszNMi=> M4q4fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9 NX\VUnBnTnWwY4Tpc44h[XO|YYm= NUX4cmpCOTVibXnudy=> MXjJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IFjERWM3KGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFHjMZBmeHSrZHWtRW1EKGG|IIP1ZpN1emG2ZTDhd5Nme3OnZDDhd{Bz\WynYYPlJI9nKEGPQzDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEGgbJIh[nliZnz1c5Jme2OnbnPlJIEtKEmFNUCgQUAxNjByNEG2JO69VS5? NWD2clhDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPFY3PzZpPkK3NVg3Pjd4PD;hQi=>
Sf9 MVrJcohq[mm2aX;uJI9nKGi3bXHu NFTpZmMyPSCvaX7z NHvQNnFKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyOCCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6|ZXP0JHNnQSClZXzsd{B2e2mwZzDBZ{1x\XC2aXTlMWFOSyCjczDzeYJ{fHKjdHWgZZN{\XO|ZXSgZZMhemWuZXHz[UBw\iCDTVOgdJJmcW6ldXLheIVlKG[xcjCxOUBucW6|IH\vcIxwf2WmIHL5JJN2[nO2cnH0[UBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjCxJIhzKGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFA1PDVizszNMi=> M1yzN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9 MV\GeY5kfGmxbjDhd5NigQ>? NVvIeINnOTVibXnudy=> Mki4TY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDDMZRmem2rbnHsJGhqey22YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQhUESDQ{ig[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCCVZkmgZ4VtdHNidYPpcochSWNvcHXweIll\S2DTVOgZZMhe3Wkc4TyZZRmKGG|c3Xzd4VlKGG|IILlcIVie2Vib3[gRW1EKHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bnd2yub4fl[EBjgSC|dXLzeJJifGViYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhNCCLQ{WwJF0hOC5yMES4OkDPxE1w NHf2cok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
A2780S NH\XcXREgXSxdH;4bYNqfHliYYPzZZk> NITSRYozPCCqcoO= M1HGTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyUzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wNFg{OiEQvF2u MlvFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O|YoRjJ5MUi2Olc3RC:jPh?=
A2780S MkfPSpVv[3Srb36gZZN{[Xl? MoW0OkBpenN? NIK5[HlKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEF{N{iwV{Bk\WyuczDhd5Nme3OnZDDhd{B1fWK3bHnuJIFk\XS7bHH0bY9vKGmwY4XiZZRm\CCob4KgOkBpenNiYomgZ5l1d2Kub4SgZZN{[XluIFXDOVAhRSByLkG1NFcyKM7:TT6= MmH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O|YoRjJ5MUi2Olc3RC:jPh?=
A2780S NFH3VYpHfW6ldHnvckBie3OjeR?= M4XjcFYhcHK| Mn7STY5pcWKrdHnvckBw\iCKRFHDNU8zNzNiaX6gbJVu[W5iQUK3PFBUKGOnbHzzJIF{e2W|c3XkJIF{KGirc4TvcoUhUDNiYXPleJlt[XSrb36gbY5kfWKjdHXkJIZweiB4IHjyd{BjgSCleYTvZoxwfCCjc4PhfUwhTUN3MDC9JFAvOTZ7NTFOwG0v MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O|Y9N2F-
Sf9 M3[5WmZ2dmO2aX;uJIF{e2G7 MUOxOUBucW6| NIe0b5hKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM2KGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFHjMZBmeHSrZHWtRW1EKGG|IIP1ZpN1emG2ZTDhd5Nme3OnZDDhd{Bz\WynYYPlJI9nKEGPQzDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEGgbJIh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6xPVA{KM7:TT6= NVzwZ4FZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPFY3PzZpPkK3NVg3Pjd4PD;hQi=>
Sf9 M2P6NmZ2dmO2aX;uJIF{e2G7 M3nye|E2KG2rboO= NUfwSpdMUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC:FLYTldo1qdmGuIFjpd{11[WepZXSgbJVu[W5icnXjc41jcW6jboSgTGRCSzRiKE[yO{B1dyBzMEi0JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgbY5{\WO2IGPmPUBk\WyuczD1d4lv\yCDYz3w[ZB1cWSnLVHNR{BieyC|dXLzeJJifGViYYPz[ZN{\WRiYYOgdoVt\WG|ZTDv[kBCVUNicILlbY5kfWKjdHXkJIZweiBzNTDtbY5{KG[xbHzve4VlKGK7IIP1ZpN1emG2LDDJR|UxKD1iMD6zN|c5KM7:TT6= M1viTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
Sf9 NF[wNWFHfW6ldHnvckBie3OjeR?= NH\repUyPSCvaX7z NW\zSpd7UW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGhqey22YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQhUESDQ{mgLFYxPCC2bzCxNFY3KHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6|ZXP0JHNnQSClZXzsd{B2e2mwZzDBZ{1x\XC2aXTlMWFOSyCjczDzeYJ{fHKjdHWgZZN{\XO|ZXSgZZMhemWuZXHz[UBw\iCDTVOgdJJmcW6ldXLheIVlKG[xcjCxOUBucW6|IH\vcIxwf2WmIHL5JJN2[nO2cnH0[UBi\GSrdHnvckBu\WG|LDDJR|UxKD1iMD64PFc5KM7:TT6= NF:yWnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
Sf9 M2f6emZ2dmO2aX;uJIF{e2G7 M4jXdlE2KG2rboO= NIHVVlBKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIILlZ49u[mmwYX70JGhFSUNzMTDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiaX7z[YN1KFOoOTDj[YxteyC3c3nu[{BC[y2yZYD0bYRmNUGPQzDhd{B{fWK|dILheIUh[XO|ZYPz[YQh[XNicnXs[YF{\SCxZjDBUWMheHKnaX7jeYJifGWmIH\vdkAyPSCvaX7zJIZwdGyxd3XkJIJ6KHO3YoP0doF1\SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAyKGi{IHL5JIZtfW:{ZYPj[Y5k\SBuIFnDOVAhRSB2LkGxNkDPxE1w NGGweG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
Sf9 MlfOSpVv[3Srb36gZZN{[Xl? NVHU[FBSOTVibXnudy=> NVX3dXZvUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQhUESDQ{egLFUyQCC2bzDlcoQhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFHjMZBmeHSrZHWtRW1EKGG|IIP1ZpN1emG2ZTDhd5Nme3OnZDDhd{Bz\WynYYPlJI9nKEGPQzDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XO3LDDJR|UxKD1iND6zOVQh|ryPLh?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O|Y9N2F-
Sf9 NEm5Sm9HfW6ldHnvckBie3OjeR?= M3;6[2lvcGmkaYTpc44hd2ZiQz30[ZJucW6jbDDIbZMufGGpZ3XkJIFv\CCFLYTldo1qdmGuIF\MRWcufGGpZ3XkJIZ2dGxibHXu[5RpKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDjc4V5eHKnc4Pl[EBqdiCoYXzsJIFzdXm5b4LtJHNnQSClZXzsd{B2e2mwZzDjZZJjd3i7Znz1c5Jme2OnaX6gLGZCVSlvbHHi[Yxm\CCjY3X0fYxifGWmLzD0dolndHWxcn;hZ4V1gWyjdHXkJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKCxiSVO1NEA:KDBwMECxJO69VS5? M4nX[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5M{e3PFY1Lz5{N{O3O|g3PDxxYU6=
Sf9 MmLySpVv[3Srb36gZZN{[Xl? MkTMOlAhdWmwcx?= NUT2e5JrUW6qaXLpeIlwdiCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOiCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMh[2:neIDy[ZN{\WRiaX6g[oFtdCCjcn35e49zdSCVZkmgZ4VtdHNidYPpcoch[2G{Yn;4fYZtfW:{ZYPj[YlvKCiIQV2pMYxi[mWuZXSgZYNmfHmuYYTl[E8hfHKrZnz1c5Jw[WOndInsZZRm\CCyZYD0bYRmKGG|IIP1ZpN1emG2ZTDh[pRmeiB4MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFAzKM7:TT6= M{mzXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5M{e3PFY1Lz5{N{O3O|g3PDxxYU6=
Sf9 NXqyPFNPTnWwY4Tpc44h[XO|YYm= NGHaOW03OCCvaX7z NELJZ2lKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM3KGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bkd2W6cILld5Nm\CCrbjDmZYxtKGG{bYn3c5JuKFOoOTDj[YxteyC3c3nu[{Bk[XKkb4j5[ox2d3Knc3PlbY4hME[DTTmtcIFj\WynZDDhZ4V1gWyjdHXkM{B1emmobIXvdo9i[2W2eXzheIVlKHCncITp[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDZyIH3pcpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6wNFIh|ryPLh?= NUCyS|FHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezO|c5PjRpPkK3N|c4QDZ2PD;hQi=>
Sf9 MlTQSpVv[3Srb36gZZN{[Xl? NIjPUpY3OCCvaX7z NEnkeXBKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygS3NVNXSjZ3fl[EBnfWyuIHzlcod1cCCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgZ49mgHC{ZYPz[YQhcW5iZnHscEBiem27d3;ycUBU\jliY3XscJMhfXOrbnegZ4Fz[m:6eX\seY9z\XOlZXnuJEhHSU1rLXzhZoVt\WRiYXPleJlt[XSnZD:geJJq\my3b4LvZYNmfHmuYYTl[EBx\XC2aXTlJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KG[udX;y[ZNk\W5uIFnDOVAhRSByLkC5NkDPxE1w NGfBZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O3O|g3PCd-MkezO|c5PjR:L3G+
Sf9 M{DrSmZ2dmO2aX;uJIF{e2G7 NX\JUpZpPjBibXnudy=> NUThVFM1UW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGhqey22YXfn[YQh\nWubDDs[Y5ofGhiaIXtZY4hemWlb33ibY5idnRiSFTBR|gh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJINw\XiycnXzd4VlKGmwIH\hcIwh[XKveYfvdo0hW2Z7IHPlcIx{KHW|aX7nJINiemKxeInmcJVwemW|Y3XpckApTkGPKT3sZYJmdGWmIHHj[ZR6dGG2ZXSvJJRzcW[udX;yc4Fk\XS7bHH0[YQheGWydHnk[UBieyC|dXLzeJJifGViYX\0[ZIhPjBibXnud{BjgSCobIXvdoV{[2WwLDDJR|UxKD1iMD6yN|Eh|ryPLh?= NYDXV3g2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezO|c5PjRpPkK3N|c4QDZ2PD;hQi=>
Sf9 MlTMSpVv[3Srb36gZZN{[Xl? M2XpfmlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDHV3QufGGpZ3XkJIFv\CCFLYTldo1qdmGuIFjpd{11[WepZXSgbJVu[W5icnXjc41jcW6jboSgTGRCSzRiKE[yO{B1dyBzMEi0JJJme2mmdXXzJEkh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJINw\XiycnXzd4VlKGmwIH\hcIwh[XKveYfvdo0hW2Z7IHPlcIx{KHW|aX7nJINiemKxeInmcJVwemW|Y3XpckApTkGPKT3sZYJmdGWmIHHj[ZR6dGG2ZXSvJJRzcW[udX;yc4Fk\XS7bHH0[YQheGWydHnk[UBieyxiSVO1NEA:KDBwM{ezJO69VS5? MmXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd|N{e4OlQoRjJ5M{e3PFY1RC:jPh?=
Sf9 NXLNNnJITnWwY4Tpc44h[XO|YYm= NF3aXVM3OCCvaX7z MlfVTY5pcWKrdHnvckBw\iCFLYTldo1qdmGuIFjpd{11[WepZXSgbJVu[W5icnXjc41jcW6jboSgTGRCSzliKE[wOEB1dyBzME[2JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgZ49mgHC{ZYPz[YQhcW5iZnHscEBiem27d3;ycUBU\jliY3XscJMhfXOrbnegZ4Fz[m:6eX\seY9z\XOlZXnuJEhHSU1rLXzhZoVt\WRiYXPleJlt[XSnZD:geJJq\my3b4LvZYNmfHmuYYTl[EBx\XC2aXTlJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6NCCLQ{WwJF0hOi54ODFOwG0v MnvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd|N{e4OlQoRjJ5M{e3PFY1RC:jPh?=
Sf9 MY\GeY5kfGmxbjDhd5NigQ>? M3rWT|YxKG2rboO= MWPJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiR2PUMZRi\2enZDDoeY1idiC{ZXPvcYJqdmGwdDDISGFEPyBqNUG4JJRwKGWwZDDy[ZNq\HWnczmg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHPv[ZhxemW|c3XkJIlvKG[jbHygZZJugXexcn2gV4Y6KGOnbHzzJJV{cW6pIHPhdoJwgHmobIXvdoV{[2WrbjCoSmFOMS2uYXLlcIVlKGGlZYT5cIF1\WRxIITybYZtfW:{b3Hj[ZR6dGG2ZXSgdIVxfGmmZTDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUAtKEmFNUCgQUAzNjh|IN88UU4> NIHBWW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O3O|g3PCd-MkezO|c5PjR:L3G+
HEK293 M4\zbmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXXycJV[PDhiaILz NVToO2tTS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDy[ZNignW{aX6gZZN{[XluIFnDOVAhRSByLkC3JO69VS5? NH3xTpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK0NVEyOid-MkiyOFEyOTJ:L3G+
NFF MYfDfZRwfG:6aXPpeJkh[XO|YYm= NUfnVYFOPzJiaILz MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOSmYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IECuNFch|ryPLh?= M{jBV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkSxNVEzLz5{OEK0NVEyOjxxYU6=
HUT78 M4HqfmFxd3C2b4Ppd{Bie3OjeR?= NViwS4xIOThiaILz MonUVJJwNWGyb4D0c5Rq[yCjY4Tpeol1gSCrbjDoeY1idiCKVWS3PEBk\WyuczDh[pRmeiBzODDodpMh[nliY3HzdIF{\S2JbH:gN{84KGG|c3H5MEBGSzVyIE2gNE4xODR|IN88UU4> M1zoRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUKyNlI4Lz5|MEGyNlIzPzxxYU6=
NFF MVfDfZRwfG:6aXPpeJkh[XO|YYm= Mkm3O|IhcHK| NYrPSpp2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVk[IIHPlcIx{KGGodHXyJFczKGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDJR|UxKD1iMD6wO{DPxE1w MoX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NEW0NFIoRjNyMkS1OFAzRC:jPh?=
HEK293 NHTqdGlEgXSxdH;4bYNqfHliYYPzZZk> MorQOFghcHK| NUHNUpI4S3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDy[ZNignW{aX6g[JlmKGKjc3XkJIF{e2G7LDDJR|UxKD1iMD6wO{DPxE1w MnjzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NEW0NFIoRjNyMkS1OFAzRC:jPh?=
M14 M1LRPWFxd3C2b4Ppd{Bie3OjeR?= M3PHXlI1KHSxIES4JIhzew>? NF74XnhKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHj1cYFvKE1zNDDj[YxteyCjc4Pld5Nm\CCjczDjZZNx[XOnIHHjeIl3cXS7IHHmeIVzKDJ2IITvJFQ5KGi{czD1d4lv\yCGRW\EJJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGK7IFHwc3RwgC2JbH:geJJqeGyneDDhd5NigQ>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5MUS2Okc,OjR2N{G0OlY9N2F-
U937 M2Pj[2Z2dmO2aX;uJIF{e2G7 NV3PTGg1OSC3TR?= MYGyOEBpenN? M2P2TWlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hXTl|NzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBqdnS{YXPlcIx2dGG{IHHj[ZR6dGG2ZXSgZYxxcGFvdIXieYxqdiCuZY\lcEBifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NFfLUFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5OFA2PSd-MkS2PVQxPTV:L3G+
U937 NFfzUIxHfW6ldHnvckBie3OjeR?= NVzYbnFuOSC3TR?= NFP5VXMzPCCqcoO= NVviSnJkUW6qaXLpeIlwdiCxZjDISGFEOSCrbjDoeY1idiCXOUO3JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGmwdILhZ4VtdHWuYYKgZYNmfHmuYYTl[EBpcXO2b37lJGg{KGyndnXsJIF1KDFidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NIPDT2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5OFA2PSd-MkS2PVQxPTV:L3G+
U937 MmPKSpVv[3Srb36gZZN{[Xl? MnXnNUB2VQ>? NIDGfZczPCCqcoO= MWLJcohq[mm2aX;uJI9nKEiGQVOyJIlvKGi3bXHuJHU6OzdiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gbY51emGlZXzseYxieiCjY3X0fYxifGWmIHjpd5RwdmViSEOgcIV3\WxiYYSgNUB2VSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7NEC1OUc,OjR4OUSwOVU9N2F-
U937 MlzCSpVv[3Srb36gZZN{[Xl? MkH3NUB2VQ>? MlmwNlQhcHK| NUn4NIxkUW6qaXLpeIlwdiCxZjDISGFEOyCrbjDoeY1idiCXOUO3JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGmwdILhZ4VtdHWuYYKgZYNmfHmuYYTl[EBpcXO2b37lJGg1KGyndnXsJIF1KDFidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NH7He|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5OFA2PSd-MkS2PVQxPTV:L3G+
MV4-11 MUnGeY5kfGmxbjDhd5NigQ>? NYfNbpBNOTBidH:gNVAxOCCwTR?= MUC2JIhzew>? MXHJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJG1XPC1zMTDj[YxteyCjc4Pld5Nm\CCjczD1dJJm\3WuYYTpc44hd2ZiYXzwbIEhfHWkdXzpckBi[2W2eXzheIlwdiCjdDCxNEB1dyBzMECwJI5OKGGodHXyJFYhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? M2nqc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NESzNFc5Lz5{NkS0N|A4QDxxYU6=
HCT116 NYnDZ5E5TnWwY4Tpc44h[XO|YYm= MYWxNEB1dyBzMECwJI5O M1LCeFYhcHK| M4HCW2lvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJJVxemWpdXzheIlwdiCxZjDhcJBp[SC2dXL1cIlvKGGlZYT5cIF1cW:wIHH0JFExKHSxIEGwNFAhdk1iYX\0[ZIhPiCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR2M{C3PEc,OjZ2NEOwO|g9N2F-
HCT116 M1K0[GZ2dmO2aX;uJIF{e2G7 MWexNEB1dyBzMECwJI5O MVK2JIhzew>? M3XOfWlvcGmkaYTpc44hd2ZiSFTBR|EwOi9|IHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4YhcGm|dH;u[UBJOyCjY3X0fYxifGmxbjDheEAyOCC2bzCxNFAxKG6PIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR2M{C3PEc,OjZ2NEOwO|g9N2F-
MV4-11 NWHueZg{TnWwY4Tpc44h[XO|YYm= MVixNEB1dyBzMECwJI5O MYm2JIhzew>? M4XaXmlvcGmkaYTpc44hd2ZiSFTBR|EwOi9|IHnuJIh2dWGwIF3WOE0yOSClZXzsd{Bie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4YhcGm|dH;u[UBJOyCjY3X0fYxifGmxbjDheEAyOCC2bzCxNFAxKG6PIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NEXI[YY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkS0N|A4QCd-Mk[0OFMxPzh:L3G+
Raji MkPMR5l1d3SxeHnjbZR6KGG|c3H5 MlftNlQhcHK| Mnj1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6 NEXJS|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G4OlY4Pid-MkexPFY3PzZ:L3G+
Ramos NUfTZZJXS3m2b4TvfIlkcXS7IHHzd4F6 NYPkVINnOjRiaILz NWTSNVdGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWmGvb4OgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O|Y9N2F-
U266 NF3VNYNEgXSxdH;4bYNqfHliYYPzZZk> NXLye5d5OjRiaILz NHXacWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWOjZ4IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[Xl? MoG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O|YoRjJ5MUi2Olc3RC:jPh?=
RPMI8226 MkD6R5l1d3SxeHnjbZR6KGG|c3H5 MX[yOEBpenN? NH;TdYxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTWE2LOEKyOkBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5 MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF6Nk[3Okc,OjdzOE[2O|Y9N2F-
HBL1 MVPDfZRwfG:6aXPpeJkh[XO|YYm= MVeyOEBpenN? M3rrNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhDVDFiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>? MoPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O|YoRjJ5MUi2Olc3RC:jPh?=
MM1S Mof3R5l1d3SxeHnjbZR6KGG|c3H5 NFv6WnozPCCqcoO= MkPUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUW0yWyClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6 MnPSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzOE[2O|YoRjJ5MUi2Olc3RC:jPh?=
OCI-LY1 NHzKOIFEgXSxdH;4bYNqfHliYYPzZZk> MVWyOEBpenN? NHHn[2lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBQS0lvTGmxJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYm= NYrI[oFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPFY3PzZpPkK3NVg3Pjd4PD;hQi=>
SUDHL4 NWnhN3pFS3m2b4TvfIlkcXS7IHHzd4F6 NYPE[mxrOjRiaILz NFrVWo5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUXUSKTESgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?= M2TlNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUi2Olc3Lz5{N{G4OlY4PjxxYU6=
MV4-11 NEjOSGtHfW6ldHnvckBie3OjeR?= MVu1NEBvVQ>? MUGyOEBpenN? MX\JcoR2[3Srb36gc4YhUESDQ{[g[IVoemGmYYTpc44hcW5iaIXtZY4hVVZ2LUGxJINmdGy|IHH0JFUxKG6PIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
MV4-11 NFX2NIRHfW6ldHnvckBie3OjeR?= NX3aS4Z6PTBibl2= MmPxNkB1dyB{NDDodpM> NF\SWoxKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKE2YND2xNUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiYXzwbIEufHWkdXzpckBpgXCncnHj[ZR6dGG2aX;uJIF1KDVyIH7NJIFnfGW{IEKgeI8hOjRiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R? Mn2wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
MV4-11 M1;RbGNmdGxiY4njcIUh[XK{ZYP0JIF{e2G7 NXjxfIo3OzBidH:gOVAhdk1? MlXCNlQhcHK| NFTKeWhE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IIP1Zk1IOSCyaHHz[UBifCB|MDD0c{A2OCCwTTDh[pRmeiB{NDDodpMh[nlicILvdIllcXWvIHnv[Ill\SC|dHHpcolv\y2kYYPl[EBndG:5IHP5eI9u\XS{aXOgcYV1cG:m MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
MV4-11 MU\BdI9xfG:|aYOgZZN{[Xl? MV6zNEBvVQ>? M1TrNFI1KHSxIES4JIhzew>? MYrJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2YND2xNUBk\WyuczDheEA{OCCwTTDh[pRmeiB{NDD0c{A1QCCqcoOgZpkhSW6wZYjpckBXNVCLIIP0ZYlvcW6pIHLhd4VlKG[ub4egZ5l1d22ndIL5 MnzZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5M{i5OVMoRjJ7N{O4PVU{RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
DNMT1 / EZH2; 

PubMed: 19279403     


Western blot of DNMT1 and EZH2 in K562 and LAMA84 following 24 hours treatment with the indicated doses of panobinostat (PS). The levels of β-actin served as the loading control.

caspase-8 / cleaved caspase-8 / Sp1; 

PubMed: 27738323     


The indicated MM cell lines were cultured for 24 hours in the absence or presence of panobinostat at the indicated concentrations. Then, the protein levels of caspase-8, cleaved caspase 8 and Sp1 were analyzed by Western blotting. 

c-Myc / IRF4; 

PubMed: 27738323     


RPMI8226 cells were cultured for 24 hours in the absence or presence of panobinostat at the indicated concentrations. Then, the protein levels of cMyc and IRF4 were analyzed by Western blotting.

Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1; 

PubMed: 28915627     


Immunoblotting of whole cell lysates prepared from MB cells treated with panobinostat. The representative western blot result shows panobinostat clearly regulated acetyl-Histone H3 (Ac-H3), cleaved caspase-3, CCND1, IDs, synaptophysin and NeuroD1.

RAD51 / BRCA1 / CHK1 / RPL13a; 

PubMed: 24244429     


CTS, OCI-AML3 or U937 AML cells were treated with variable concentrations of panobinostat for 48 h. Whole cell lysates were subjected to Western blotting to measure protein levels for BRCA1, CHK1, and RAD51 in the cells.

H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP; 

PubMed: 31071955     


Immunoblot analyses of histone acetylation (H3K9AC, H3K18AC, H3K56AC, H4K8AC and H4K16AC), cell cycle arrest (p27 and p27) and apoptotic-related protein (C-Caspase 3 and C-PARP) expression following various panobinostat treatments for 6 h and 24 h on K562 cells. H3, H4 and GAPDH immunoblots served as internal controls.

19279403 27738323 28915627 24244429 31071955
Immunofluorescence
Synaptophysin / NACM; 

PubMed: 28915627     


Panobinostat treated cells are positive for (B) synaptophysin (red) and (C) NCAM (red). Scale bars, 50 μm.

α-tubulin / Acetyl-α-tubulin; 

PubMed: 29983882     


CLBL-1 cells were treated with 10 nM of panobinostat for 24 h and then microtubules were visualized by immunofluorescence labeling using antibodies against tubulin and acetylated tubulin (ac-tubulin) using the appropriate excitation and emission filters as described in the material and methods section. Representative microphotographs with tubulin and ac-tubulin labeling (green) and DAPI stained-nuclei (blue) at 100× magnification are shown. Scale bar, 5 μm.

BiP; 

PubMed: 23544167     


Immunofluorescence analysis of BiP after 24 and 48 hours of treatment in HepG2 (upper panel) and Hep3B (lower panel) cells with 0.1 µM panobinostat and 10 nM thapsigargin, showing an increase and a different protein distribution in panobinostat-treated cells, comparable to 10 nM thapsigargin effect, used as a positive control. Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is x630, and scale bar represents 10 µm.

ATF4; 

PubMed: 23544167     


HepG2 (left panel) and Hep3B (right panel) cells were treated for 24 and 48 hours with 0.1 µM panobinostat and the immunofluorescence results show an increase of ATF4 and its nuclear localization in both cell lines. Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is x630, and scale bar represents 10 µm.

IRE1α / S724-IRE1α; 

PubMed: 23544167     


Analysis of IRE1α/XBP1 involvement. Immunofluorescence results of IRE1α and its phosphorylated form in HepG2 (A) and Hep3B (B) cell lines after 24 and 48 hours of treatment with 0.1 µM panobinostat. The results indicate that panobinostat induces an increase of both the total and the phosphorylated form of IRE1α and a spotted distribution of this protein (enlargement showed for phospho-IRE1α, B). Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is x630, and scale bar represents 10 µm.

28915627 29983882 23544167
Growth inhibition assay
Cell viability; 

PubMed: 27738323     


The indicated MM cell lines were cultured in triplicate in the absence or presence of panobinostat at the indicated concentrations. After culturing for 48 hours, cell viability was measured by a WST-8 cell proliferation assay. Results were expressed as the mean +/− SD.

27738323
In vivo In lung cancer and mesothelioma animal models, LBH589 markedly decreases tumor growth by 62%. LBH589 is equally effective in immunocompetent and severe combined immunodeficien-cymice, suggesting that the inhibition of tumor growth by LBH589 is not due to direct immunologic effects. Daily LBH589, given i.p. at 20 mg/kg for 5 days per week, leading to an average decrease in growth of 70%. Compared with the corresponding control tumors, LBH589 leads to a 53% decrease for H526-derived tumors, an 81% decrease for BK-T-derived tumors, a 76% decrease for RG-1- derived tumors, and a 70% decrease for H69-derived tumors. In contrast to the lack of tumor regression notes in NSCLC and Meso-derived xenografted tumors that are treated under identical conditions and doses, LBH589 results in dramatic tumor regression in SCLC-derived tumors and RG-1-derived tumor. [2]

Protocol

Cell Research:[1]
- Collapse
  • Cell lines: MOLT-4 cell lines and Reh (pre-B cells)
  • Concentrations: 50 nM
  • Incubation Time: 48 hours
  • Method: Untreated and LBH589-treated cells [human Ph- acute lymphoblastic leukemia MOLT-4 (T cells) and Reh (pre-B cells)] are stained with annexin V and propidium iodide using annexin V-FITC apoptosis detection kit I. The percentage of apoptotic and nonviable cells is determined by flow cytometry. At least 5 × 104 cells are collected with a CyAn ADP Violet cytometer. Percentage apoptosis is calculated considering all the annexin V-positive plus the annexin V/PI-positive cells; percentage loss of cell viability is calculated considering all the annexin V-positive plus the PI-positive and the annexinV/PI-positive cells.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: Severe combined immunodeficiency (SCID) mice with M30 (107 cells) or A549 (5 × 106 cells), H69 (2.5 × 106 cells), BK-T (6.5 × 106), H526 (10 × 106), and RG1 (10 × 106) cells
  • Dosages: 10 mg/kg, 20 mg/kg
  • Administration: Administered via i.p. injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 69 mg/mL (197.46 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+48% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 349.43
Formula

C21H23N3O2

CAS No. 404950-80-7
Storage powder
in solvent
Synonyms NVP-LBH589
Smiles CC1=C(C2=CC=CC=C2N1)CCNCC3=CC=C(C=C3)C=CC(=O)NO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04341311 Active not recruiting Drug: Marizomib|Drug: Panobinostat Diffuse Intrinsic Pontine Glioma|Pediatric Brainstem Glioma|Pediatric Brainstem Gliosarcoma Recurrent|Pediatric Cancer|Pediatric Brain Tumor|Diffuse Glioma Dana-Farber Cancer Institute|Celgene|Secura Bio Inc. August 10 2020 Phase 1
NCT03632317 Withdrawn Drug: Panobinostat|Drug: Everolimus Glioma|Diffuse Intrinsic Pontine Glioma University of Michigan Rogel Cancer Center October 2019 Phase 2
NCT03982134 Withdrawn Drug: PDR001|Drug: Panobinostat Melanoma|Non Small Cell Lung Cancer Muhammad Furqan|Novartis Pharmaceuticals|University of Iowa September 2019 Phase 1
NCT04326764 Recruiting Drug: Panobinostat Acute Myeloid Leukaemia (AML)|Myelodysplastic Syndromes (MDS) Goethe University|Jan J. Cornelissen (HOVON-SAKK)|Sebastian Giebel (PALG) July 24 2018 Phase 3
NCT03515915 Unknown status -- Patients With Recurrent or Refractory Multiple Myeloma University Hospital Montpellier|Poitiers University Hospital April 23 2018 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How to reconstitute the compound for in vivo mice study?

  • Answer:

    We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

Related HDAC Products

Tags: buy Panobinostat (LBH589) | Panobinostat (LBH589) supplier | purchase Panobinostat (LBH589) | Panobinostat (LBH589) cost | Panobinostat (LBH589) manufacturer | order Panobinostat (LBH589) | Panobinostat (LBH589) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID