Panobinostat (LBH589)

Catalog No.S1030 Synonyms: NVP-LBH589

For research use only.

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.

Panobinostat (LBH589) Chemical Structure

CAS No. 404950-80-7

Selleck's Panobinostat (LBH589) has been cited by 320 publications

Purity & Quality Control

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Biological Activity

Description Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Targets
HDAC (MOLT-4 cells) [1] HDAC (Reh cells) [1]
5 nM 20 nM
In vitro

LBH589 induces apoptosis among MOLT-4 and Reh cells in a time- and dose-dependent manner. Moreover, LBH589 is more potent in MOLT-4 than in Reh cells. LBH589 markedly prevents the growth of both MOLT-4 and Reh cells in a dose-dependent manner at 48 hours. LBH589 treatment causes a 2- to 3-fold increase in the number of cells in the G2/M phase of the cell cycle compared with the control cells. LBH589 is associated with induction of histone H3K9 and histone H4K8 acetylation as well as decreasing levels of c-Myc expression in a dose-dependent manner. LBH589 treatment also increases the levels of p21 expression. LBH589 treatment also decreases the levels of c-Myc after an initial increase at the lowest dose (10 nM) in Reh cells. In addition, LBH589 gives rise to substantial increases in mRNA levels of proapoptosis and DNA repair genes. LBH589 induces increased levels of acetylated histone H3 and H4 at the GADD45G promoter. [1] Besides, LBH589 inhibits growth of non small cell lung cancer cell lines (such as human H1299, L55 and A549 with IC50 of 5 nM, 11 nM and 30 nM, respectively), mesothelioma (such as human OK-6 and Ok-5 with IC50 of 5 nM and 7 nM, respectively) and small cell lung cancer cell lines (such as human RG-1 and LD-T with IC50 of 4 nM and 5 nM, respectively). [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT29 NWfmXG5xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUKwMVExKM7:TR?= NHvRVnIxNTRiZB?= MYfpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NH;IPXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewNlc5PCd-Mk[3NFI4QDR:L3G+
HepG2 NHy3[IJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHlVIwxNTFyIN88US=> MVywMVQh\A>? NV\LdllxcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MmPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HT29 NYPk[ZpbTnWwY4Tpc44hSXO|YYm= M2fKVVUxyqCwTR?= M1HL[FI1NTd{IHi= Mm\mbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UhOyCjZoTldkA1QMLiaNMg MnX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HepG2 NILIVJJHfW6ldHnvckBCe3OjeR?= MWW1NOKhdk1? MWKyOE04OiCq MUPpcoR2[2WmIHHjeIl3[XSrb36gc4Yh[2G|cHHz[UA{KGGodHXyJFI1yqCqwrC= M4XGNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{CyO|g1Lz5{NkewNlc5PDxxYU6=
HCC827 M3\6RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2S0VFUwPy53L{GwJI5O M2PjZVczyqCq MW\EUXNQ NX3RZmFE\W6qYX7j[ZMhfGinIHHueIlxem:uaX\ldoF1cX[nIHXm[oVkfCCxZjDldoxwfGmwaXK= NUnMOFNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2O|U1QDRpPkK2Olc2PDh2PD;hQi=>
A549  MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfsVJA2OTBxMUWvNlAhdk1? NV;2SpRsPzMEoHi= M4rLd2ROW09? M2[5N4VvcGGwY3XzJJRp\SCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5Qhd2ZiZYLsc5Rqdmmk NGfrTVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[3OVQ5PCd-Mk[2O|U1QDR:L3G+
NCI-H460  MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn:2NVAwOjBxM{Cgcm0> NHrZW4s4OsLiaB?= NVvpcpJ4TE2VTx?= M2jiPYVvcGGwY3XzJJRp\SCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5Qhd2ZiZYLsc5Rqdmmk M2iwfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Nke1OFg1Lz5{Nk[3OVQ5PDxxYU6=
J89GFP M2HvTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnqzSG1UV8Li M{XwVmVEPTB;NEmuPFUhyrFiMUKuOlUhdk1? NVPDN3F[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlM2PjhpPkK2OVY{PTZ6PD;hQi=>
THP89GFP MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVH2cmtiTE2VT9Mg NEHzRZZGSzVyPUG5MlM1KMLzIE[uOFMhdk1? NEPxbmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2N|U3QCd-Mk[1OlM2Pjh:L3G+
SK-NEP-1 MnTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTNUVB7OC5yMfMAl|ExNjBizszN NE\zRokzPCCq M2jyTGROW00EoB?= NH\TVJJKSzVyPUe2MlM1KG6P M2fmU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
G401 NHqyO2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fnd|AvODIkgKOxNE4xKM7:TR?= NWnBSXI{OjRiaB?= M4DQU2ROW00EoB?= M365NWlEPTB;MUSzMlAzKG6P MlHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
SK-NEP-1 NH33dYhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M4HyclUxKG6P MkG1NgKBmzRiZB?= NELjSJFFVVORwrC= M1XReJJm\HWlZYOgZ4VtdCC|dYL2bZZidCCrbjDhJJRqdWViZHXw[Y5l\W62IH3hco5meg>? M3\vO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
G401 MWTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NUHCe|F2PTBibl2= M2nOXlHjiJN2IHS= NFTyTZZFVVORwrC= MnTndoVlfWOnczDj[YxtKHO3co\peoFtKGmwIHGgeIlu\SCmZYDlcoRmdnRibXHucoVz M1W3N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUe2NlE6Lz5{NkG3OlIyQTxxYU6=
SK-NEP-1 NGfUW3hCeG:ydH;zbZMhSXO|YYm= M{DGZ|UxNzFyMDDuUS=> NUPUe|EyOjRiaB?= NFG1fIJFVVORwrC= MWfpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 MXrBdI9xfG:|aYOgRZN{[Xl? MlvMOVAwOTByIH7N MWKyOEBp NWTTfW4{TE2VT9Mg NXLvV5BJcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MmjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
SK-NEP-1 NWPKblh{TnWwY4Tpc44hSXO|YYm= MmDPOVAwOTByIH7N MmXmNlQhcA>? MnLDSG1UV8Li M4TiVZNpd3e|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25? NVfjdHRoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 MVjGeY5kfGmxbjDBd5NigQ>? MnvSOVAwOTByIH7N M1nJVFI1KGh? NFXFdFdFVVORwrC= M{nkPZNpd3e|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
SK-NEP-1 MonsSpVv[3Srb36gRZN{[Xl? NWPqc|RrPTBxMUCwJI5O NVXDNY55OjRiaB?= NGCyeYRFVVORwrC= NYCxSYh6cW6mdXPld{Bk\WyuIHP5Z4xmKGSrc3;y[IVzyqB? NIP1OZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
G401 NY\w[JBiTnWwY4Tpc44hSXO|YYm= MXy1NE8yODBibl2= M3SzRlI1KGh? NV;ySo1CTE2VT9Mg MX7pcoR2[2W|IHPlcIwh[3mlbHWg[Il{d3KmZYNCpC=> NFW2SHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
RPMI 8226 Mlj3R4VtdCCVdYL2bZZidCCDc4PhfS=> NHPvNGkzNzRxNjDuUS=> Mnv0OFjjiImq MX7pcoR2[2W|IHGgd4lodmmoaXPhcpQh\GWlcnXhd4UhcW5idHjlJINmdGxiZ4Lve5Rp Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
OPM2 MUPD[YxtKFO3co\peoFtKEG|c3H5 NWPBWJpCOi92L{[gcm0> NVrFRnVtPDkkgJno NHX6boVqdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq NVfNUIRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFAzQTJpPkK2NFAxOjl{PD;hQi=>
U266 NX[zeoZRS2WubDDTeZJ3cX[jbDDBd5NigQ>? MWeyM|QwPiCwTR?= M{L6W|Q56oDLaB?= M3S5c4lv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
H929 M{O2emNmdGxiU4Xyeol3[WxiQYPzZZk> MkXkNk81NzZibl2= MkOwOFjjiImq MYXpcoR2[2W|IHGgd4lodmmoaXPhcpQh\GWlcnXhd4UhcW5idHjlJINmdGxiZ4Lve5Rp MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
RPMI 8226  MVzBdI9xfG:|aYOgRZN{[Xl? MlH4OQKBkW6P MVWyOE81QCCq MVvpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGmwIHGgeIlu\S2mZYDlcoRmdnRibXHucoVz MnWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
HCC827 NIDKVXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljrNVAhdk1? MYC0PEBp NW\Ie2tqTE2VTx?= M{XxT4VvcGGwY3XzJINqe3CuYYTpckB{\W6|aYTpeol1gcLi MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl2NE[xO{c,OjV7NES2NVc9N2F-
NCI-H23 NYX5XGRtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfpNVAhdk1? MmnHOFghcA>? NHHvXJlFVVOR M3;nNYVvcGGwY3XzJINqe3CuYYTpckB{\W6|aYTpeol1gcLi NH21V4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0OFYyPyd-MkW5OFQ3OTd:L3G+
AML3 NESwRllHfW6ldHnvckBCe3OjeR?= NFXnXIMxNTFizszN NIP2T5AzPMLiaB?= MnfTbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NUC0PZE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
ML-1 M16zc2Z2dmO2aX;uJGF{e2G7 MmXWNE0yKM7:TR?= NETGTnkzPMLiaB?= MlTIbY5lfWOnczDEUmEh\nKjZ33lcpRifGmxbtMgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NVq2cVVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
RPMI-8226vr10  M1r1PGZ2dmO2aX;uJGF{e2G7 NFzHOW0xNTFizszN NXzvcJd6OjUEoHi= NV\pdnplcW6mdXPld{BFVkFiZoLh[41mdnSjdHnvcuKhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1L6[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
ML-1 NEjQNJhHfW6ldHnvckBCe3OjeR?= MXqxJO69VQ>? M2jTOFI1yqCq NVjnU2RZcW6lcnXhd4V{KGOjc4Dhd4UuOyCjY4Tpeol1gSB2LX\vcIQ> M{\3eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
RPMI-8226vr10  NUTUNphMTnWwY4Tpc44hSXO|YYm= MlPmNUDPxE1? M1\FbFI1yqCq NIfDRWhqdmO{ZXHz[ZMh[2G|cHHz[U0{KGGldHn2bZR6KDJwNT3mc4xl M3H2N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
SK-N-BE (2) NHT0RpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnexNlTjiImq MXHJR|UxRTFyND6w5qCKyrIkgJm3Mlghdk1? NIXVTW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), PAN  MK NVTtXJhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1n6O|I16oDLaB?= NHXJdVRKSzVyPUGwOE4x6oDLwsJihKk4Njhibl2= Ml7SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-BE (2), MK  PAN NYXBZ|R3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TQSVI16oDLaB?= MWnJR|UxRTN6Mj6w5qCKyrIkgJm0N{4zKG6P NHTjdFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-AS MlvKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUn4SWR3OjUkgJno MnuyTWM2OD1|Nz6x5qCKyrIkgJmyMlQhdk1? NX3GeW9zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N|A5QTF4PD;hQi=>
SK-N-DZ MnG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXSyOQKBkWh? NYnmN5RSUUN3ME2xO{4y6oDLwsJihKkxNjRibl2= NG\WT449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
Caki-1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoD4NVAwOjVxNUCgcm0> NV3lfGEyPDhiaB?= NInvTldqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meiC|eX7ldodqe3SrY3HscJkhf2m2aDDybZRwdmG4aYK= NYDWSJRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO|kyQTFpPkK1Nlc6OTlzPD;hQi=>
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HCC-LM3 NGj3SmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXO0[GwxOS1zMECwJI5O MUSyOE81QC95MjDo M1rHRmROW09? M3\nPIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M2S2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
HepG2 M{nubGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYj4PFZIOS1zMECwJI5O MVqyOE81QC95MjDo Ml\zSG1UVw>? MYDpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3zPW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
SMMC-7721 NHTaRYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV[xMVExODBibl2= MlfyNlQwPDhxN{KgbC=> NHiw[5dFVVOR M2XuNYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NIfwNlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3 NIPDVIVCeG:ydH;zbZMhSXO|YYm= MV:1NEBvVQ>? M1O2VVQ5KGh? MonVSG1UVw>? MYjpcoR2[2W|IHPlcIwh[XCxcITvd4l{KHOrZ37p[olk[W62bImgbY4h[SClYYPwZZNmNWSncHXu[IVvfCCvYX7u[ZIh[nliY3zlZZZi\2Vib3[gZ4F{eGG|ZYOgN{whQCCjbnSgPS=> MoXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HepG2 MULBdI9xfG:|aYOgRZN{[Xl? NVvxTlNyPTBibl2= M1vzflQ5KGh? MVzEUXNQ NWrVU|B2cW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzbYdvcW[rY3HueIx6KGmwIHGgZ4F{eGG|ZT3k[ZBmdmSnboSgcYFvdmW{IHL5JINt\WG4YXflJI9nKGOjc4Dhd4V{KDNuIEigZY5lKDl? M4rQOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
SMMC-7721 MYjBdI9xfG:|aYOgRZN{[Xl? NHfWToI2OCCwTR?= MkXWOFghcA>? NVfWWlVZTE2VTx?= NUPTZWhrcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzbYdvcW[rY3HueIx6KGmwIHGgZ4F{eGG|ZT3k[ZBmdmSnboSgcYFvdmW{IHL5JINt\WG4YXflJI9nKGOjc4Dhd4V{KDNuIEigZY5lKDl? NF\rVVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3 Mn3SSpVv[3Srb36gRZN{[Xl? M{CxeVUxNzFyMDDuUS=> MmGyNlQhcA>? NHvGWHVFVVOR M4PIPYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC= MkXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
HepG2 MV\GeY5kfGmxbjDBd5NigQ>? MojmOVAwOTByIH7N MmnKNlQhcA>? NF3JfodFVVOR M1zqOYRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIICtV3RCXDNiYX7kJJAuSWu2wrC= MoT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
SMMC-7721 MkHwSpVv[3Srb36gRZN{[Xl? MV21NE8yODBibl2= MWSyOEBp MlH3SG1UVw>? MlHU[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicD3TWGFVOyCjbnSgdE1Cc3UEoB?= NIO3U4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HCC-LM3 M4XUR2Z2dmO2aX;uJGF{e2G7 MW[1NE8yODBibl2= NYDHbZg1OjRiaB?= NYPhbXFiTE2VTx?= MYPkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v NEPab449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
HepG2 MW\GeY5kfGmxbjDBd5NigQ>? NGL6SHM2OC9zMECgcm0> NIDhd5gzPCCq MV;EUXNQ NEO0V4Vld3ewcnXneYxifGW|IFLjcE15VCCneIDy[ZN{cW:w M4jWXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEmzPVU3Lz5{NEC5N|k2PjxxYU6=
SMMC-7721 NHvPUHRHfW6ldHnvckBCe3OjeR?= M2r1SlUxNzFyMDDuUS=> MYiyOEBp NUn4b3VyTE2VTx?= MXHkc5dvemWpdXzheIV{KEKlbD34UEBmgHC{ZYPzbY9v NIXB[|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5N|k2Pid-MkSwPVM6PTZ:L3G+
FaDu M2rpT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLlNVAx6oDHbl2= NUW1Z4J4QC9zMD:xNkBp M1fUfoRqe3CuYYnl[EBiKHOrZ37p[olk[W62IHHu[EBxem:ub37n[YQhTzJxTTDhdpJme3RiYYSgPEBidmRiMUNihKVpKHCxc4SgdoVt\WG|ZR?= NFL0OI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyOlQ5Oid-MkSwNlY1QDJ:L3G+
FaDu NWL5S4xxTnWwY4Tpc44hSXO|YYm= NHXuSlEyODEkgJXuUS=> M{i4O|IwPC96L{GyJIg> M4PnfIlv\HWlZXSgdFIyX2GoMT;DbZAyyqCneIDy[ZN{cW:w M1u1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK2OFgzLz5{NECyOlQ5OjxxYU6=
PC-3  M{nXOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LaO|AuOTBizszN MVKyOE81QC95MjDo M3\QeYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZT2gZY5lKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NUXNbFUzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PVEzOTZpPkKzPVkyOjF4PD;hQi=>
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RWPE-1  NEfRZo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPsdXdjOC1{MDFOwG0> M3jWfVI1NzR6L{eyJIg> NFftXZFqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M3O1d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUmxNlE3Lz5{M{m5NVIyPjxxYU6=
Capan-1 NVjV[ldWTnWwY4Tpc44hSXO|YYm= NFv0PWQzPS93MD:xNFAhdk1? MXG4M|I1NzR6IHi= NVKwR5p{TE2VTx?= NHHlTFZld3ewcnXneYxifGWmIGLvckBuWk6DIHHu[EBxem:2ZXnuJIV5eHKnc4Ppc44h[W6mIHTve45{fHKnYX2gd4lodmGuaX7n NGDNTnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{myNlg5Pid-MkO5NlI5QDZ:L3G+
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CFPAC-1  MYHGeY5kfGmxbjDBd5NigQ>? NHnrW5IzPS93MD:xNFAhdk1? MoDzPE8zPC92ODDo MlrVSG1UVw>? MnvW[I94dnKnZ4XsZZRm\CCUb36gcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO|aX;uJIFv\CCmb4fud5Rz\WGvIIPp[45idGmwZx?= Mly4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MkK4PFYoRjJ|OUKyPFg3RC:jPh?=
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CFPAC-1  NEXsUnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXobXdSOjVxNUCvNVAxKG6P NFnMc4E1QCCq Ml\rSG1UVw>? NYraN2tSemWmdXPld{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NYL6fFA6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NlI5QDZpPkKzPVIzQDh4PD;hQi=>
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HN22 Mn\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\u[Xc6OC1{MDDuUS=> Mm[2NlQwPDhiaB?= M3XCbmROW09? MVHpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCrbjDic5RpKHSrbXWtJIFv\CCmb4PlMUBl\XCnbnTlcpQhdWGwbnXy MkHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{eyN|UoRjJ|OEe3NlM2RC:jPh?=
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HN22 M4DoUmFxd3C2b4Ppd{BCe3OjeR?= M2\TSVAuOjBibl2= MlHyOFghcA>? NUO1PWd4TE2VTx?= NXLlN3BXcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HSC4  NF[wXpNCeG:ydH;zbZMhSXO|YYm= M3\jflAuOjBibl2= M3X6ZlQ5KGh? M1XlcWROW09? MoDEbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? M1npbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe3NlM2Lz5{M{i3O|I{PTxxYU6=
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HN22 MkPwSpVv[3Srb36gRZN{[Xl? NF2wT3UxNTJyIH7N MoP5OFghcA>? MmPZSG1UVw>? NYXISWRbe3WycILld5NmeyCVcEGg[ZhxemW|c3nvcuKh MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
HSC4  M4Kwe2Z2dmO2aX;uJGF{e2G7 NUTsTmdbOC1{MDDuUS=> MmfaOFghcA>? MkO0SG1UVw>? NH3HOmR{fXCycnXzd4V{KFOyMTDlfJBz\XO|aX;uxsA> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5N{KzOUc,OjN6N{eyN|U9N2F-
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Assay
Methods Test Index PMID
Western blot DNMT1 / EZH2 ; caspase-8 / cleaved caspase-8 / Sp1 ; c-Myc / IRF4 ; Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1 ; RAD51 / BRCA1 / CHK1 / RPL13a ; H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP 19279403 27738323 28915627 24244429 31071955
Immunofluorescence Synaptophysin / NACM ; α-tubulin / Acetyl-α-tubulin ; BiP ; ATF4 ; IRE1α / S724-IRE1α 28915627 29983882 23544167
Growth inhibition assay Cell viability 27738323
In vivo In lung cancer and mesothelioma animal models, LBH589 markedly decreases tumor growth by 62%. LBH589 is equally effective in immunocompetent and severe combined immunodeficien-cymice, suggesting that the inhibition of tumor growth by LBH589 is not due to direct immunologic effects. Daily LBH589, given i.p. at 20 mg/kg for 5 days per week, leading to an average decrease in growth of 70%. Compared with the corresponding control tumors, LBH589 leads to a 53% decrease for H526-derived tumors, an 81% decrease for BK-T-derived tumors, a 76% decrease for RG-1- derived tumors, and a 70% decrease for H69-derived tumors. In contrast to the lack of tumor regression notes in NSCLC and Meso-derived xenografted tumors that are treated under identical conditions and doses, LBH589 results in dramatic tumor regression in SCLC-derived tumors and RG-1-derived tumor. [2]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: MOLT-4 cell lines and Reh (pre-B cells)
  • Concentrations: 50 nM
  • Incubation Time: 48 hours
  • Method: Untreated and LBH589-treated cells [human Ph- acute lymphoblastic leukemia MOLT-4 (T cells) and Reh (pre-B cells)] are stained with annexin V and propidium iodide using annexin V-FITC apoptosis detection kit I. The percentage of apoptotic and nonviable cells is determined by flow cytometry. At least 5 × 104 cells are collected with a CyAn ADP Violet cytometer. Percentage apoptosis is calculated considering all the annexin V-positive plus the annexin V/PI-positive cells; percentage loss of cell viability is calculated considering all the annexin V-positive plus the PI-positive and the annexinV/PI-positive cells.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Severe combined immunodeficiency (SCID) mice with M30 (107 cells) or A549 (5 × 106 cells), H69 (2.5 × 106 cells), BK-T (6.5 × 106), H526 (10 × 106), and RG1 (10 × 106) cells
  • Dosages: 10 mg/kg, 20 mg/kg
  • Administration: Administered via i.p. injection
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 69 mg/mL
(197.46 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+48% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 349.43
Formula

C21H23N3O2

CAS No. 404950-80-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C2=CC=CC=C2N1)CCNCC3=CC=C(C=C3)C=CC(=O)NO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04341311 Active not recruiting Drug: Marizomib|Drug: Panobinostat Diffuse Intrinsic Pontine Glioma|Pediatric Brainstem Glioma|Pediatric Brainstem Gliosarcoma Recurrent|Pediatric Cancer|Pediatric Brain Tumor|Diffuse Glioma Dana-Farber Cancer Institute|Celgene|Secura Bio Inc. August 10 2020 Phase 1
NCT03632317 Withdrawn Drug: Panobinostat|Drug: Everolimus Glioma|Diffuse Intrinsic Pontine Glioma University of Michigan Rogel Cancer Center October 2019 Phase 2
NCT03982134 Withdrawn Drug: PDR001|Drug: Panobinostat Melanoma|Non Small Cell Lung Cancer Muhammad Furqan|Novartis Pharmaceuticals|University of Iowa September 2019 Phase 1
NCT04326764 Recruiting Drug: Panobinostat Acute Myeloid Leukaemia (AML)|Myelodysplastic Syndromes (MDS) Goethe University|Jan J. Cornelissen (HOVON-SAKK)|Sebastian Giebel (PALG) July 24 2018 Phase 3
NCT03515915 Unknown status -- Patients With Recurrent or Refractory Multiple Myeloma University Hospital Montpellier|Poitiers University Hospital April 23 2018 --

(data from https://clinicaltrials.gov, updated on 2021-09-06)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo mice study?

Answer:
We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

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