4-Phenylbutyric acid (4-PBA)

Catalog No.S3592 Synonyms: Benzenebutyric acid

For research use only.

4-Phenylbutyric acid (4-PBA, Benzenebutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.

4-Phenylbutyric acid (4-PBA) Chemical Structure

CAS No. 1821-12-1

Selleck's 4-Phenylbutyric acid (4-PBA) has been cited by 4 Publications

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Biological Activity

Description 4-Phenylbutyric acid (4-PBA, Benzenebutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.
Targets
HDAC [1]
In vitro

4-PBA inhibits the ER stress induced by SiNPs in RAW264.7 cells, as evidenced by the expansion and degranulation of ER, as well as greatly up-regulated Bip and CHOP expressions.[3]

In vivo

4-PBA attenuates LPS-induced bone loss in lipopolysaccharide (LPS)-treated mice, by increasing area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments.[4]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: Mouse macrophage cell line, RAW264.7 cells
  • Concentrations: 3 mM
  • Incubation Time: 6 h
  • Method:

    Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 164.20
Formula

C10H12O2

CAS No. 1821-12-1
Storage 3 years -20°C powder
2 years -80°C in solvent

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00067756 Completed Drug: 4-PBA Alpha 1-Antitrypsin Deficiency University of Florida|Alpha-1 Foundation|Brantly Mark L. M.D. November 2001 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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