For research use only.

Catalog No.S8648

2 publications

ACY-738 Chemical Structure

CAS No. 1375465-91-0

ACY-738 inhibits HDAC6 with low nanomolar potency (IC50=1.7 nM) and a selectivity of 60- to 1500-fold over class I HDACs.

Selleck's ACY-738 has been cited by 2 publications

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Biological Activity

Description ACY-738 inhibits HDAC6 with low nanomolar potency (IC50=1.7 nM) and a selectivity of 60- to 1500-fold over class I HDACs.
HDAC6 [1]
1.7 nM
Methods Test Index PMID
Western blot
acet α-tubulin; 

PubMed: 27957719     

Dose-response of trichostatin A (TSA), ACY-738, or ACY-775 on acetylation of α-tubulin was established in N2a cells and determined by Western blot.

acet H3 / acet α-tubulin ; 

PubMed: 27957719     

Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.

In vivo ACY-738 induces dramatic increases in α-tubulin acetylation in brain and stimulate mouse exploratory behaviors in novel, but not familiar environments. ACY-738 has the antidepressant-like properties. Elimination of ACY-738 from plasma is rapid, with plasmatic half-life of 12 min and concentration below 10 ng/ml after 2 h. ACY-738 rapidly distributes to the brain leading to a total drug exposure in CNS comparable to that of peripheral tissues[1]. ACY-738 decreases several characteristics of SLE (Systemic lupus erythematosus) in NZB/W mice by dictating B cell development in the bone marrow. ACY-738 treatment increases the percentage of B cells in early developmental stages, while decreasing the percentage of cells in late pre-B cell fraction F. ACY-738 regulation of BM B cell development could be due to regulation developmental checkpoints known to be dysfunctional during SLE[2].


Cell Research:


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  • Cell lines: Undifferentiated RN46A-B14 cells
  • Concentrations: 2.5 μM
  • Incubation Time: 4 h
  • Method:

    Undifferentiated RN46A-B14 cells, a line of immortalized rat raphe neuronal precursors, are grown. They are treated with 2.5 μM ACY-738, ACY-775, tubastatin A, or 0.6 μM TSA, or vehicle (0.1% DMSO) for 4 h. Samples are processed using Epigentek histone extraction kit and quantified using protein assay.

    (Only for Reference)
Animal Research:


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  • Animal Models: Male NIH Swiss mice
  • Dosages: 5 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (199.78 mM)
Ethanol 1 mg/mL (3.69 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 270.29


CAS No. 1375465-91-0
Storage powder
in solvent
Synonyms N/A
Smiles C1CC1(C2=CC=CC=C2)NC3=NC=C(C=N3)C(=O)NO

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HDAC Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID