Catalog No.S8648

For research use only.

ACY-738 inhibits HDAC6 with low nanomolar potency (IC50=1.7 nM) and a selectivity of 60- to 1500-fold over class I HDACs.

ACY-738 Chemical Structure

CAS No. 1375465-91-0

Selleck's ACY-738 has been cited by 2 Publications

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Biological Activity

Description ACY-738 inhibits HDAC6 with low nanomolar potency (IC50=1.7 nM) and a selectivity of 60- to 1500-fold over class I HDACs.
HDAC6 [1]
1.7 nM
Methods Test Index PMID
Western blot acet α-tubulin 27957719
Immunofluorescence acet H3 / acet α-tubulin 27957719
In vivo ACY-738 induces dramatic increases in α-tubulin acetylation in brain and stimulate mouse exploratory behaviors in novel, but not familiar environments. ACY-738 has the antidepressant-like properties. Elimination of ACY-738 from plasma is rapid, with plasmatic half-life of 12 min and concentration below 10 ng/ml after 2 h. ACY-738 rapidly distributes to the brain leading to a total drug exposure in CNS comparable to that of peripheral tissues[1]. ACY-738 decreases several characteristics of SLE (Systemic lupus erythematosus) in NZB/W mice by dictating B cell development in the bone marrow. ACY-738 treatment increases the percentage of B cells in early developmental stages, while decreasing the percentage of cells in late pre-B cell fraction F. ACY-738 regulation of BM B cell development could be due to regulation developmental checkpoints known to be dysfunctional during SLE[2].

Protocol (from reference)

Cell Research:


  • Cell lines: Undifferentiated RN46A-B14 cells
  • Concentrations: 2.5 μM
  • Incubation Time: 4 h
  • Method:

    Undifferentiated RN46A-B14 cells, a line of immortalized rat raphe neuronal precursors, are grown. They are treated with 2.5 μM ACY-738, ACY-775, tubastatin A, or 0.6 μM TSA, or vehicle (0.1% DMSO) for 4 h. Samples are processed using Epigentek histone extraction kit and quantified using protein assay.

Animal Research:


  • Animal Models: Male NIH Swiss mice
  • Dosages: 5 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 270.29


CAS No. 1375465-91-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CC1(C2=CC=CC=C2)NC3=NC=C(C=N3)C(=O)NO

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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