Choose Your Country or Region

Trichostatin A (TSA)

Name: Trichostatin A (TSA)
Brand: Selleck Chemicals
SKU: S1045
Rating: 5 (203 reviews)

Catalog No.S1045

For research use only.

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

CAS No. 58880-19-6

Selleck's Trichostatin A (TSA) has been cited by 203 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Related Compound Libraries

Other HDAC Products

Download Inhibitor Catalog

HDAC Signaling Pathway Map

Biological Activity

Description

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

Targets

HDAC ^[1]
(Cell-free assay)

~1.8 nM

In vitro

Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. ^[1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. ^[2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HeLa	NWDhWIRpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MnTRNVAwOzBxNUCgcm0>	NEXWdGo4OiCq	NGfPVXFFVVOR	MXPpcoNz\WG\|ZYOgeIhmKG63bXLldkBw\iCPTWCgLO6V\|qivKTDsc5N{KGOnbHzzJIRwe2ViZHXw[Y5l\W62bIm=	NWC4N4lERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXKyNEBvVQ>?	NFPXUpQ4OiCq	M3nkd2ROW09?	NVXE[FZjUUN3MDDv[kAzOG6P	M3PuelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MU[1O\|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa	NYPQS5dlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUTtOYdFPDBibl2=	NFSyTIU1QCCq	Ml7zSG1UVw>?	NYLLfolqUUN3MDDv[kA1OG6P	M4\yXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MU[1O\|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa	M{[wdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MX2xNFAhdk1?	MYGyOEBp	M4H3XmROW09?	Mlj2TWM2OCCxZjCxNFBvVQ>?	NYnOUYdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
Caco-2	MlPrSpVv[3Srb36gRZN{[Xl?	NHjaVoYyKML3TdMg	NFvUVY4zPCCq		MX;k[YNz\WG\|ZYOgV2VTXCCycn;0[YlvKGW6cILld5Nqd25?	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7NUC3NEc,OjNzOUWwO\|A9N2F-
HTK	M4DYZ2Z2dmO2aX;uJGF{e2G7	MnLiNVAxNThyMH7N	NG\ER484OiCq		MV7icI9kc3NiVFfGMe6z6oDVSX7keYNm\CCUT2OgZY5lKEh{T{NCpGFk[3WvdXzheIlwdg>?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
HTK	MlfMSpVv[3Srb36gRZN{[Xl?	NVmyUHB[PDByIH7N	NXTqR5FOPzJiaB?=		NU\oUGpEcW6qaXLpeJMhXEeILd8y5qCUUW6mdXPl[EBOgW:oaXLyc4Jt[XO2IFTp[oZmemWwdHnheIlwdg>?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
HEK293	NX;VUmQ{TnWwY4Tpc44hSXO\|YYm=	MYSxJOK2VcLi	MnOyNVghcMLi		NV[0WIxmcW6qaXLpeJMhfGinIHvhcIlzcW5vNz3t[YRq[XSnZDDy[YNzfWm2bXXueEBw\iC\|eX7wbIltcW5vMTDh[4dz\WejdHXzJIlvfG9iYXfndoV{d22ncx?=	M1L1bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki0PFQ5Lz5{M{K4OFg1QDxxYU6=
SH-SY5Y	M3XXcGZ2dmO2aX;uJGF{e2G7	MXeyOVAhdk1?	NXvFfFk2OTZiaB?=		MVfjbIFv\2W\|IHX4dJJme3Orb36gdIF1fGW{bjDv[kBo\W6nczDpcpZwdH[nZDDpckBkcG:uZYP0[ZJwdCC\|eX70bIV{cXNuIIXweIFs\SCjbnSg[YZndHW6	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{NkSyNkc,OjN\|Mk[0NlI9N2F-
THP1	NHSzc4pCeG:ydH;zbZMhSXO\|YYm=	NUGwO5RQOSEQvF2=	NWe1VXNZOjRiaB?=		MWjpcoR2[2W\|wrDj[YxtKGSnYYTo	NXviTVh6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
K562	MoPzRZBweHSxc3nzJGF{e2G7	M4TQdFEh\|ryP	MXSyOEBp		MlnNbY5lfWOnc9MgZ4VtdCCmZXH0bC=>	NXLiSpJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
Kazumi	Mnz1RZBweHSxc3nzJGF{e2G7	M3LET\|Eh\|ryP	M2XjZ\|I1KGh?		M4nBPIlv\HWlZYRCpINmdGxiZHXheIg>	M1;lfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NECwOVE6Lz5{M{SwNFUyQTxxYU6=
KG1	Mm\iRZBweHSxc3nzJGF{e2G7	MWixJO69VQ>?	NFnvR\|MzPCCq		NUSwZmxJcW6mdXPld:Kh[2WubDDk[YF1cA>?	Ml24QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEC1NVkoRjJ\|NECwOVE6RC:jPh?=
HL60	MWfBdI9xfG:\|aYOgRZN{[Xl?	M1zQeVEh\|ryP	M3rDOlI1KGh?		MlnsbY5lfWOnc9MgZ4VtdCCmZXH0bC=>	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRyMEWxPUc,OjN2MEC1NVk9N2F-
HEL	NFrycYhHfW6ldHnvckBCe3OjeR?=	NI\2R\|gxNjVizszN	NYXMcpRkOjRiaB?=	NEW0UGNmfGijbn;s	NFnpTJRl\WO{ZXHz[ZMhfGinIHXufplu\SCjY4Tpeol1gSCxZjDISGFEeyClbz30doVifG2nboSge4l1cCCldYLjeY1qdg>?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR\|MEm1O{c,OjN2M{C5OVc9N2F-
K562	MlW2SpVv[3Srb36gRZN{[Xl?	MkTONE42KM7:TR?=	M4n5[FI1KGh?	Mn;W[ZRp[W6xbB?=	NV;mRYU2\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5?	MnP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2M{C5OVcoRjJ\|NEOwPVU4RC:jPh?=
LM8	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NITGOVUxNjNizszN	Mk[xNVIuQTZiaB?=	M1fCXWROW09?	NVi1dox5cW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJINwNXS{ZXH0cYVvfCC5aYToJI1mfG[xcn3pci=>	M2\xR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEWxPFE4Lz5{M{S1NVgyPzxxYU6=
MG-63	NX;6ZpQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmLpNE4{KM7:TR?=	NHTJWJgyOi17NjDo	NIjndWZFVVOR	Mn7xbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHPvMZRz\WG2bXXueEB4cXSqIH3leIZwem2rbh?=	M{jhPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEWxPFE4Lz5{M{S1NVgyPzxxYU6=
H1299	MXnGeY5kfGmxbjDBd5NigQ>?	NGfaVW4xNjVizszN	M{DyNlQ5KGh?	MmXuSG1UVw>?	NYr5VlJ4cW6qaXLpeJMhfGinIHnueoF{cX[nbnXzd:KhKGOxbXLpcoUhf2m2aDDzbYxq[mmwaX6=	MkG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NkG5O\|UoRjJ\|NE[xPVc2RC:jPh?=
H1299	MmnrSpVv[3Srb36gRZN{[Xl?	MXuwMlUh\|ryP	MX20PEBp	NX3YOVJITE2VTx?=	MkTBbY5pcWKrdIOgeIhmKG2rZ4LheI9zgSCyb4TlcpRq[WxiY3;tZolv\SC5aYToJJNqdGmkaX7pci=>	M2TGOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NE[xPVc2Lz5{M{S2NVk4PTxxYU6=
H1299	M2PNeWZ2dmO2aX;uJGF{e2G7	NYfKXVBNOC5\|M,MAl\|HDqML3TR?=	M3TrflI1NzR6IHi=	NWDUbXB5TE2VTx?=	MUfpcoNz\WG\|ZYOgSU1k[WSqZYLpckBxem:2ZXnuJIxmfmWuczDkc5NmKGSncHXu[IVvfGy7	NGKwc\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S2NVk4PSd-MkO0OlE6PzV:L3G+
MCF7	M375XWZ2dmO2aX;uJGF{e2G7	MWqwMlA2NTJizszN	NHrjU5IzPCCq		M2W5NZJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDwOVMu\GWyZX7k[Y51KGenbnXzJIJ6KE63dHzpck0{	Mnr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{C1OFAoRjJ\|NEewOVQxRC:jPh?=
ARN8	MmjOSpVv[3Srb36gRZN{[Xl?	MVKwMlA2NTJizszN	NUnNb4ZoOjRiaB?=		MkfadoVlfWOnczD0bIUhcW6mdXP0bY9vKG:oIIC1N{1l\XCnbnTlcpQh\2WwZYOgZpkhVnW2bHnuMVM>	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR5MEW0NEc,OjN2N{C1OFA9N2F-
U373	MlrGSpVv[3Srb36gRZN{[Xl?	NVP4TFd4OC5{NT:wMlUwOSEQvF2=	MVWyOEBp		NIXiN4ZqdmO{ZXHz[ZMhWFJvQjDlfJBz\XO\|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFVCgmGmQx?=	M4mzbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEe0NVcyLz5{M{S3OFE4OTxxYU6=
HASMCs	M3vvZmZ2dmO2aX;uJGF{e2G7	NEfpcJQxNTVyMDDuUS=>	NUHEN3pCPiCm		MXjkc5dvNXKnZ4XsZZRmeyC2aHWg[ZhxemW\|c3nvckBw\iCKQVTDJIFv\CClZXzsJJZq[WKrbHn0fS=>	M1XJUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUG4OFY4Lz5{M{WxPFQ3PzxxYU6=
MFE296	NGTpSmFHfW6ldHnvckBCe3OjeR?=	NGLpSGY2ODBibl2=	MlzIOUBl	NESyWVVFVVOR	NGPlb5hqdmS3Y3XzJGwyS0GPwrDlfJBz\XO\|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?=	MoLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ\|NUOwO\|Y6RC:jPh?=
EN1	NYnqZ\|RCTnWwY4Tpc44hSXO\|YYm=	M{\D[FUxOCCwTR?=	NYL0W4psPSCm	M{f4fWROW09?	NIPObGtqdmS3Y3XzJGwyS0GPwrDlfJBz\XO\|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?=	NW[xWoI1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N\|A4PjlpPkKzOVMxPzZ7PD;hQi=>
HEC1A	M1\uO2Z2dmO2aX;uJGF{e2G7	Mn7qOVAxKG6P	MoXaOUBl	M{W4RWROW09?	M3vDXIlv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF	MnrsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ\|NUOwO\|Y6RC:jPh?=
ECC1	MW\GeY5kfGmxbjDBd5NigQ>?	NXLHPHhSPTByIH7N	NXzBb3BzPSCm	MX\EUXNQ	MnnIbY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN?	MoXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ\|NUOwO\|Y6RC:jPh?=
AGS	MnPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MX[wMlAyPS1zIN88US=>	NF;yV2E4OiCq		NYHrdnlMcW6mdXPld{Bk\WyuIHTlZZRpKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk>	NY[wc445RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OFUxOjRpPkKzO\|Q2ODJ2PD;hQi=>
Huh7	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUfPUodWOSEQvF2=	M1XYb\|I1KGh?	MYHEUXNQ	M3\QZpJm\HWlZYOgZYJwfXRiMkClJJZq[WKrbHn0feKh	MnXmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NEO5N\|MoRjJ\|NkSzPVM{RC:jPh?=
Lovo	M3H2bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MljsNE4zNzFwMD:1MlAh\|ryP	MX[xNk8zPC9\|Nj:0PEBp		NXrmSHgycW6mdXPld{Bk\WyuIHTlZZRpKGmwIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI>	MlnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5N{CwNFAoRjJ\|N{ewNFAxRC:jPh?=
HCT1	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mn\kNE4zNzFwMD:1MlAh\|ryP	NFfWOWkyOi9{ND:zOk81QCCq		Mni3bY5lfWOnczDj[YxtKGSnYYToJIlvKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK=	M3qwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{ewNFAxLz5{M{e3NFAxODxxYU6=
SUM190PT	MoDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MoP5OVAwOTByL{K1NEBvVQ>?	NVizU4pEPDhiaB?=	NWT1[oJ4TE2VTx?=	MlLvbY5lfWOnczDj[YxtKGSnYYToJIF1KDVyIH7NJJNmdnOrdHn2[Yx6	NYC2Xpc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI3OzhpPkKzO\|kzPjN6PD;hQi=>
SUM149PT	NUjieZBsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NI\CS40zNzdwNT:xNEDPxE1?	NGjDXGk1QCCq	M1\6NGROW09?	NHnzWYxqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6\|ZX7zbZRqfmWueR?=	M2HOblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{myOlM5Lz5{M{e5NlY{QDxxYU6=
H1299	NHL4PZBCeG:ydH;zbZMhSXO\|YYm=	MWmwMlUwOSEQvF2=	NHvaOlY1QCCq	MWjleIhidm:u	MoLGbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	M3\IUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OE[3PVkyLz5{M{i2O\|k6OTxxYU6=
A549	M{XIcmFxd3C2b4Ppd{BCe3OjeR?=	MV6wMlUwOSEQvF2=	NH:xW\|U1QCCq	M4DCS4V1cGGwb3y=	NYPobFZtcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4N{m5NUc,OjN6Nke5PVE9N2F-
H1299	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3\XeVAvOjVxMD61M\|Eh\|ryP	NUnZO3VxOjRxNEivO\|IhcA>?	NIrZSnhmfGijbn;s	MkfEbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnuJINwdmOnboTyZZRqd25iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4N{m5NUc,OjN6Nke5PVE9N2F-
A549	MlXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1zjb\|AvOjVxMD61M\|Eh\|ryP	MY[yOE81QC95MjDo	M4\yNIV1cGGwb3y=	M33DOIlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDpckBkd26lZX70doF1cW:wIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ?	NGjxdIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i2O\|k6OSd-MkO4Olc6QTF:L3G+
H1299	NFTaWnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVuxxsDPxE4EoB?=	MVSyOE81QC95MjDo	NYK5fHRz\XSqYX7vcC=>	M3[wcYlv[3KnYYPld{Bk\WyuIHTlZZRpKGGodHXyJFI1cA>?	MnfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MU[2NFkoRjJ\|OUG2OlA6RC:jPh?=
PMNs	NGLyR3lHfW6ldHnvckBCe3OjeR?=	NXr2boo6OzBibl2=	NH7LdIY1QCCq		M2S1fIlvcGmkaYTzJIVn\mW{b3P5eI9{cXNuIFjERWMh[WO2aY\peJkh[W6mIGLhZ{Bi[3Srdnn0fS=>	NI\DNI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m4PFYyPyd-MkO5PFg3OTd:L3G+
CA46	NVjCWoZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NIPwRmE{NzZxMUKvNlQwPDhibl2=	Ml\2OFghcA>?		NIHqdGlqdmirYnn0d{B1cGViY3XscEBxem:uaX\ldoF1cW:wIHH0JFI1KGGwZDC0PEBvVcLi	MmTxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNkS5OVEoRjJ2ME[0PVUyRC:jPh?=
HCT116	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M2r6[lAvOiEQvF2=	MnTwNVIhcA>?		M13PPYVvcGGwY3XzJINmdGxiYYDvdJRwe2m\|IHnu[JVk\WRiYomgdoFlcWG2aX;u	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF{MkKzNUc,OjRzMkKyN\|E9N2F-
HLEB3	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MlqxNE41NzBwODFOwG0>	MV[0PEBp	MmH4SG1UVw>?	Mmr5bY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	MkPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUe4O\|goRjJ2MUW3PFc5RC:jPh?=
SRA01/04	M1rpfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{jzUVAvPC9yLkig{txO	MknKOFghcA>?	MXTEUXNQ	M4XKTIlv\HWlZYOgZ4VtdCCjcH;weI9{cXN?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O\|g9N2F-
HLEB3	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYKwMlIh\|ryP	NYjPSWdiPDhiaB?=	NV2yZVdMTE2VTx?=	NXjlfGwxe3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC\|dYDwdoV{e2mwZzD0bIUhWEl\|Sz;Bb5QtKHB\|OF3BVGsh[W6mIFXST\|EwOiC\|aXfuZYxqdmdicHH0bJdigXN?	NFfnbnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1O\|g4QCd-MkSxOVc5Pzh:L3G+
SRA01/04	NXLXOXBiT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NV7wUGtyOC5{IN88US=>	NYPqZWwzPDhiaB?=	NHzoO21FVVOR	NUHYUIdDe3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC\|dYDwdoV{e2mwZzD0bIUhWEl\|Sz;Bb5QtKHB\|OF3BVGsh[W6mIFXST\|EwOiC\|aXfuZYxqdmdicHH0bJdigXN?	NWrUOndoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
A2780	M4W1NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2q5XlAvODVvMjFOwG0>	M4DOZ\|ExNzJ2L{S4JIg>	M{XzfGROW09?	M4XVWI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{	NV[2foNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlM5ODFpPkK0NlI{QDBzPD;hQi=>
SKOV3	MmKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M17T[VAvODVvMjFOwG0>	NX21[\|FvOTBxMkSvOFghcA>?	MWTEUXNQ	NU\4RoRxdWWmaXH0[ZMh\3Kxd4ToJIFzemW\|dDDpckBiKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK=	NHLadWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN\|gxOSd-MkSyNlM5ODF:L3G+
Huh7	MUPGeY5kfGmxbjDBd5NigQ>?	NFjsTY8xNjFxMD61M\|EvOCEQvF2=	NEHZSIwzPGh?		NEDjOHd{fXCycnXzd4V{KFmWSFTDNkBo\W6nIHX4dJJme3Orb36=	M{O2VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[5OlczLz5{NEK2PVY4OjxxYU6=
hMSCs	M{XYeGZ2dmO2aX;uJGF{e2G7	NF;ZTZQ3NjJ3IH7N	NXzkfVQxOjRiaB?=	NULsSVBOTE2VTx?=	MWDzeIFjcWyrenXzJGhqe3SxbnWgRYNmfHmuYYTpc44h[W6mIITo[UBGgHC{ZYPzbY9vKG:oIGDseZJqeG:2ZX70JGdmdmW\|	NXLxZ3JIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVI{PTZpPkK0N\|EzOzV4PD;hQi=>
HT29	NUH0WYlIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVuxPFAhdk1?	NIXMU5M4OiCq		NYq3T3ZYUUN3MDDv[kAyQDBibl2=	M2m5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[4NlY2Lz5{NEO2PFI3PTxxYU6=
RPE	NHP1cYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3;jdFAvOi9yLkSvNE45NzFizszN	NUDRbWhROjRxNEivO\|IhcA>?	MV3EUXNQ	NVHXOWlWcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44h[nliY3XscEBkgWOuZTDhdpJme3R?	NGKwVI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1OlYxOid-MkS0OVY3ODJ:L3G+
U87 GBM	M{\ReWZ2dmO2aX;uJGF{e2G7	MVOxNFAwPTByIF7t	NF7ve4w1QCCq	NWHYfoFLOTByJTDleIhidm:u	MV3JcoR2[2W\|IGPlcoV{[2WwY3WtUIls\SCDbITldoF1cW:wczDpckBPfWOuZXHyJG1wenCqb3zv[5k>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
U87 GBM	M1nEOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXHHSoJpOTByL{OwNE82ODBibl2=	Mlj3O\|IhcA>?	NGHtOIgyODBnIHX0bIFvd2x?	Mmr6doVlfWOnczDt[YFvKGOnbHygcpVu[mW{IHL5JFMyNCB3NDygZY5lKDV6wrCl	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
ACP03	Mn7LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NGHVT5czPTBxM{WwM\|UxOG6P	NFvqXYYzPGh?		Mn\v[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>?	MmTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Nki1OFcoRjJ2Nk[4OVQ4RC:jPh?=
ACP02	NITvNpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MX2yOVAwOzVyL{WwNI5O	M{DYTlI1cA>?		NX7rWplm\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JI9nKGGycILvfIlu[XSnbImgO\|DDqCViYYSgNlUxyqCwTR?=	NEjEeXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
HeLa	NIHseY5HfW6ldHnvckBCe3OjeR?=	NW[yXHlYOW2P	NWGzdZJVOC53aB?=		NGPSRYhi[m:uaYPo[ZMhfGinIITveIFtKEiGQVOgZYN1cX[rdIm=	MmS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MEe0O\|QoRjJ2N{C3OFc1RC:jPh?=
HMEC-1	MVfGeY5kfGmxbjDBd5NigQ>?	MlrUN\|Axdk1?	M4HifFI1cA>?		M3\kRolv[3KnYYPld{BmgHC{ZYPzbY9vKG:owrDWSWdHWjQEoH3SUmE>	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzME[zNUc,OjR5MUC2N\|E9N2F-
HeLa	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGjsXZgz\|ryP	MnjnOFhp		MYTk[YNz\WG\|ZYOgZ4VtdCC4aXHibYxqfHliYXLveZQhOjVn	NHXDS\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0OlE{PSd-MkS4OFYyOzV:L3G+
LNCaP	NGXDTJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NF3OdHcyODBvMUCwNI5O	MWOyOIg>		MlHpTWM2OCCxZjCzNFBvVQ>?	NYfveHBYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVQ3PThpPkK0PFU1PjV6PD;hQi=>
PC3	MnXqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4jSZVExOC1zMECwcm0>	NF;RUGMzPGh?		M3nWTWlEPTBib3[gN\|Axdk1?	NIrVenc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi1OFY2QCd-MkS4OVQ3PTh:L3G+
CNE2	NFflOZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXGxNFAuPjBybl2=	NFrkXFgzPC92OD:3Nog>		MV\pcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK=	NILnZoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2PVkxOSd-MkS5Olk6ODF:L3G+
HeLa	NHLwOGdHfW6ldHnvckBCe3OjeR?=	NFvW[Y4zPTCwTR?=	M1zWclE3cA>?	MlWxSG1UVw>?	MojqbY5kemWjc3XzJGN[WDGDMTDtVm5CKGW6cILld5Nqd28EoB?=	NGL6RWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxOlY5QCd-MkWxNVY3QDh:L3G+
A549	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MV:wMlAyNTBwOd88US=>	NGjaS\|Q4Omh?	MXLEUXNQ	MY\JR\|UxKG:oIECuNFbPxE1?	Mn\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
SKOV-3	MkDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4TEfFAvODFvMD65{txO	NX6yfGJ6PzKq	M4jDSmROW09?	NYTYb20{UUN3MDDv[kAxNjN{zszN	M1TCVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
A549	MlP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3PiUFAvOS1zzszN	NUjabFR3PDiq	MnfuSG1UVw>?	MoDmTWM2OCCxZjCwMlI5\|ryP	M1[1elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
SKOV-3	M2PSe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Ml\WNE4yNTIQvF2=	MVq0PIg>	M1fjbWROW09?	MkTXTWM2OCCxZjCwMlfPxE1?	NVzpWW9bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
A549	NGrpWVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NIKyfI4yNTFyzszN	MWeyOIg>	NYn5ZnpWTE2VTx?=	NF;UZ\|dKSzVyIH;mJFMvOs7:TR?=	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
SKOV-3	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MX6xMVEx\|ryP	MnK5NlRp	M4TMc2ROW09?	MYXJR\|UxKG:oIEWuOu69VQ>?	NIPEWIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
MCF7	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NVL1S4hYOC14MEDuUS=>	Mn[5NlRp	NF\V[XRFVVOR	NF3aWYNKSzVyIH;mJFc2dk1?	Ml\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUK3NlEoRjJ3MUmyO\|IyRC:jPh?=
MDA-MB-231	Mk\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MX6wMVYxOG6P	MX6yOIg>	NXzDV2hFTE2VTx?=	MY\JR\|UxKG:oIEGwNI5O	NYTXUGh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPVI4OjFpPkK1NVkzPzJzPD;hQi=>
A431	NVXZNnNoTnWwY4Tpc44hSXO\|YYm=	MYO1NI5O	NWey[Ih{Oi94L{GyM\|I1cA>?	NVLhU2NuTE2VTx?=	NEDsOG5i[3SrdnH0[ZMheDJzIHHu[EBqdmirYnn0d{BCXEZ\|IHX4dJJme3Orb36=	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5MUC2PUc,OjV\|N{GwOlk9N2F-
A431	M4\sXmFxd3C2b4Ppd{BCe3OjeR?=	M1vkR\|IwOTBxNUCvNVAxdk1?	NUfqOlB4PDiq	MlyxSG1UVw>?	MXnpcohq[mm2czD0bIUh[2WubDDndo94fGh?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5MUC2PUc,OjV\|N{GwOlk9N2F-
PC3	NH\2RnlHfW6ldHnvckBCe3OjeR?=	NE\QbnUxNjIQvF2=	M1XIXFQ5cA>?	MVzEUXNQ	NWXGUoh7cW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR\|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?=	Mo\SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
SW480	MlSzSpVv[3Srb36gRZN{[Xl?	MlXiNE4y\|ryP	MV20PIg>	M2r4OGROW09?	MVjpcoR2[2W\|IHnuZ5Jm[XOnIH;mJGhFSUNzIHHu[EBJTEGFMjDvckBUdHWpIHflcoV{KHC{b33veIVz	Mn3pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
PC3	NHHZ[VVHfW6ldHnvckBCe3OjeR?=	NYXJPWhjOC5zzszN	NGTJZ\|A1QGh?	MV3EUXNQ	MnnEZZR1\W63YYTld{Bqdn[jc3nvckBidmRibXnndoF1cW:w	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
PC3	MYjGeY5kfGmxbjDBd5NigQ>?	MUSwMlHPxE1?	NGiz[4c1QGh?	Mmf4SG1UVw>?	MoHTdoV3\XK\|ZYOgSW1V	NVfOPVN{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480	M2rLTWZ2dmO2aX;uJGF{e2G7	MUCwMlHPxE1?	M4rnd\|Q5cA>?	NF;EO\|hFVVOR	MYfheJRmdnWjdHXzJIlvfmG\|aX;uJIFv\CCvaXfyZZRqd25?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
SW480	NEjy[GJHfW6ldHnvckBCe3OjeR?=	M1XSUFAvOc7:TR?=	M4\KfFQ5cA>?	Mnn4SG1UVw>?	NXfhTZhNemW4ZYLz[ZMhTU2W	MkLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
MEFs	NX7lSpRLTnWwY4Tpc44hSXO\|YYm=	M4jxSVXPxE1?	M2nH[\|E3cA>?		NUjTU3FXcW6lcnXhd4V{KHSqZTDFVGVEKGG2dHHjbI1mdnRuIGTpdkBl\WyrdnXyfUBidmRidHjlJIVn\mmlaXXuZ5khd2ZicHXk[ZN1[WxiZn;ycYF1cW:w	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6Mk[zOEc,OjV2OEK2N\|Q9N2F-
TE13	NILPWIdCeG:ydH;zbZMhSXO\|YYm=	NGi2dnkxNjQQvF2=	NYHhOVRuOjSq		M37J[4lvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25?	MmnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3N{m2OlUoRjJ3NUe5OlY2RC:jPh?=
TE13	M2qxTGZ2dmO2aX;uJGF{e2G7	NXvUfWlbOC5\|zszN	MmLqNlRp		MX\1dE1z\We3bHH0[ZMhWkGVU1[1RUBt\X[nbB?=	M2HVXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUe5OlY2Lz5{NUW3PVY3PTxxYU6=
HEK 293	MVPGeY5kfGmxbjDBd5NigQ>?	NF;1WIExNjgQvF2=	NGPEUFAzPGh?	MUTleIhidm:u	NWPaeFVX\W6qYX7j[ZMhTU6jQzDhZ4V1gWyjdHnvckBidmRiaX7jdoVie2W\|IFXOZWMh[WK3bnThcoNmKGmwIITo[UB1d3SjbDDj[YxtKGy7c3H0[UBidmRiYYSgeIhmKGOnbHygd5Vz\mGlZR?=	MnPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5OEewO\|koRjJ3N{i3NFc6RC:jPh?=
MDA-MB-231	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGH1SI0zPS12MECgcm0>	MXS0PEBp		MV\JR\|UxKG:oIEK2N{4zdk4EoB?=	M1zaXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEW1NVk5Lz5{M{C1OVE6QDxxYU6=
MCF-7	M1jkZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmjUNlUuPDByIH7N	NVnwcopRPDhiaB?=		NVWyfWhmUUN3MDDv[kAzOjBwNH7N	NFmzfY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C1OVE6QCd-MkOwOVUyQTh:L3G+
ECC-1	NHHVXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVOxNFAhdk1?	Mn\JNlQhcA>?	MYXleIhidm:u	NHLBSFhqdmO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh[XCxcITveIlkKG63Y3zlbUB1dyB\|NTW=	M3Gwc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK4PFA{Lz5{M{CyPFgxOzxxYU6=
HEC-1A	M4rvbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NFPLbG4yODBibl2=	MUeyOEBp	NVvqc\|Fq\XSqYX7vcC=>	MnL3bY5kemWjc3XzJJRp\SCycn;wc5J1cW:wIH;mJIFxd3C2b4TpZ{BvfWOuZXmgeI8hOzln	M4n5flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK4PFA{Lz5{M{CyPFgxOzxxYU6=
NHAC-kn	M4XkeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NX3uV2VbOTBxMUCwM\|UxOCCwTR?=	M4fwSVEzKGh?	M3zHT2ROW09?	M1XVNWlEPTBib3[gOVAxdk1?	NV;vNpJtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVc5PzFpPkKzNFE4QDdzPD;hQi=>
A549	M1fDfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEfPU4wzPTBibl2=	NVrZR5pKPi15MjDo	NGPQTVNFVVOR	MkHSZ4F2e2W\|IHGg[5Jm[XSncjDpcohq[mm2b4L5JIVn\mWldDDjc41jcW6nIIfpeIghXFiWIH;yJIVzdG:2aX7pZi=>	NEX0[Y89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm5OFc5OCd-MkK5PVQ4QDB:L3G+
MG-63	MnjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NIfKXVE{ODBibl2=	M37t[\|EzKGh?	NXnYWpAzTE2VTx?=	NGTZSHJqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hQDZn	Mn\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5OUmzN\|goRjJ{N{m5N\|M5RC:jPh?=
MG-63	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH\ZeVY{ODBibl2=	MmTsNlQhcA>?	NEL1W5RFVVOR	MYXpcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gOlcm	M2HFWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{m5N\|M5Lz5{Mke5PVM{QDxxYU6=
MG-63	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NF3DTHk{ODBibl2=	MVW0PEBp	MVPEUXNQ	NELSelRqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPTZn	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd7OUOzPEc,OjJ5OUmzN\|g9N2F-
HL60	MlXMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYX4co01OTVyLUO1NEBvVQ>?	MUOyOEBp		MYPpcoNz\WG\|ZYOgZ4VtdCCjcH;weI9{cXQEoIfo[Y4h[2:wY3XueJJifGmxboOgbIlocGW{IIToZY4hOjVyIH7N	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3M{ezPUc,OjJ5NUO3N\|k9N2F-
U937	M1Tae2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVe0TZNROTVyLUO1NEBvVQ>?	NH3lbIwzPCCq		NIG3fI1qdmO{ZXHz[ZMh[2WubDDhdI9xfG:\|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3M{ezPUc,OjJ5NUO3N\|k9N2F-
SCC-6	NX\ueoNNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVSyNFAuOzJyMDDuUS=>	NH23[HoyOi9{ND:0PEBp	NFr1SnlFVVOR	NYX0NY9KcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hd2ZiU1PDMVYh[2WubIOgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy	NFjSdlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW1NlMzOSd-MkK1OVI{OjF:L3G+
U87	Mmq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHzaNFkyODBvM{CwJI5o	NF\lSHYzPCCq		NGS3N2tqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPzJnIDDheEAzODCwZx?=	NUW4cWhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyO\|A5PDlpPkKyNlcxQDR7PD;hQi=>
K562	M3yyc2Z2dmO2aX;uJGF{e2G7	NFnlSYEyKM7:TR?=	MmXFNVIhcA>?	NFjnWHdFVVOR	MU\lcohidmOnczD0bIUh\XiycnXzd4lwdiCxZjDSWW5ZOyCrbnT1Z4VlKGK7IEWtZZpiNUOmUh?=	NXjXNopPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxO\|kyQThpPkKyNVc6OTl6PD;hQi=>
Reh	M1zufmZ2dmO2aX;uJGF{e2G7	MWmwMlMwOSEQvF2=	NH63cngyOiCq	MlriSG1UVw>?	NITUNFRmdmijbnPld{B1cGViZYjwdoV{e2mxbjDv[kBTXU6[MzDpcoR2[2WmIHL5JFUu[XqjLVPkVi=>	M1PMS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUe5NVk5Lz5{MkG3PVE6QDxxYU6=
Mv1Lu	MYXGeY5kfGmxbjDhd5NigQ>?		NID3dHkyQCCqcoO=		NXyz[GhiUW6mdXP0bY9vKG:oIIfpcIQufHmyZTDwNlEheHKxbX;0[ZIh[WO2aY\heIlwdiCrbjDtbY5sKE24MVz1JINmdGy\|IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6KHKnbHH0bZZmKHSxIHLhd4FtKGyndnXsMEBGSzFyMECgQUAxNjF7IN88UU4>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh6MUKzNkc,OTd6OEGyN\|I9N2F-
HeLa	NHTLNGNHfW6ldHnvckBie3OjeR?=		MlLLNlQhcHK\|		NI\EWWVKdmS3Y4Tpc44hd2ZicEKxJIV5eHKnc4Ppc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjZoTldkAzPCCqcoOsJGVEOTByMDC9JFAvODFzNTFOwG0v	M{HYUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{m3PFI3Lz5zOEO5O\|gzPjxxYU6=
Mv1Lu	NFHYcXhHfW6ldHnvckBie3OjeR?=		NEnwdZozPCCqcoO=		NYTlZnhFUW6lcnXhd4UhcW5iaIXtZY4hf2muZDD0fZBmKHB{MTDwdo91\WmwIHX4dJJme3Orb36gbY4hdWmwazDNelFNfSClZXzsd{Bi\nSncjCyOEBpenNiYomgdFIyKHC{b33veIVzKGG\|c3H5MEBGSzFyMECgQUAxNjB{IN88UU4>	M4rkUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm3OVc5Lz5{NEm5O\|U4QDxxYU6=
HCT 116	NFrO[VFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				M3[x[mlvKH[rdILvJIFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iY3;sc44h[2GwY3XyJGhEXCBzMU[gZ4VtdHNiZHX0[ZJucW6nZDDifUBOVVRiYYPzZZktKEWFNUCgQUAyKM7:TT6=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV7N{SxN{c,OTF3OUe0NVM9N2F-
T24	NF;zT5VHfW6ldHnvckBie3OjeR?=				NYjhd2ExS2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBz\XG3aYLl[EBnd3JiYXPleJlt[XSrb36gc4YhcGm\|dH;u[U01KGmwIHj1cYFvKFR{NDDjZY5k\XJiY3XscJMtKEWFNUCgQUAyKM7:TT6=	NEfWWmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5O\|QyOyd-MUG1PVc1OTN:L3G+
T24	MWrGeY5kfGmxbjDhd5NigQ>?				M{nRfmlvcGmkaYTpc44hd2ZiYXPleJlt[XSrb36gc4YhcGm\|dH;u[U01KGmwIHj1cYFvKFRvMkSgZ4Fv[2W{IHPlcIx{NCCHQ{WwJF0hOSEQvF2u	NGDMXWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNE[xN\|MyOid-MUS2NVM{OTJ:L3G+
T24	MVfGeY5kfGmxbjDhd5NigQ>?				NWjiXYQzUW6qaXLpeIlwdiCxZjDhZ4V1gWyjdHnvckBw\iCqaYP0c45mNTRiaX6gbJVu[W5iVD2yOEBk[W6lZYKgZ4VtdHNuIFXDOVAhRSB2IN88UU4>	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDZzM{OxNkc,OTR4MUOzNVI9N2F-
T24	MV7GeY5kfGmxbjDhd5NigQ>?				NFSxRZZKdmS3Y4Tpc44hd2ZiYXzwbIEhfHWkdXzpckBi[2W2eXzheIlwdiCrbjDoeY1idiCWMkSgZ4VtdHNuIFXDOVAhRSByLkCxJO69VS5?	M2TJcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUGxOFY3Lz5zOUGxNVQ3PjxxYU6=
T24	Ml24SpVv[3Srb36gZZN{[Xl?				NHHBW3dKdmS3Y4Tpc44hd2ZiaHnzeI9v\SCKMzDhZ4V1gWyjdHnvckBqdiCqdX3hckBVOjRiY3XscJMtKEWFNUCgQUAxNjBzIN88UU4>	M4\BTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUGxOFY3Lz5zOUGxNVQ3PjxxYU6=
HepG2	Mlz2SpVv[3Srb36gZZN{[Xl?				M{PlOXVxNXKnZ4XsZZRqd25ib3[geJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IH;mJIh2dWGwIFPMRU0yKHC{b33veIVzKHKnZ3nvckAuOTB3NTD0c{AuPjKkcDD0doFve2[nY4Tl[EBqdiCKZYDHNkBk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO4JO69VS5?	M4fSNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW2NVAzLz5{NUW1OlExOjxxYU6=
HeLa	MYDGeY5kfGmxbjDhd5NigQ>?				MUXJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJGhmVGFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB1fWK3bHnuJIRm[WOndInsZZRqd25iaX7jeYJifGWmIH\vdkBwfmW{bnnnbJQh[nliY1XMTXNCNCCHQ{WwJF0hOC5yMEWyJO69VS5?	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzMUmxPUc,OjZ4MUG5NVk9N2F-
HL60	NH;zfoZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M4DW[lI1KGi{cx?=		NUniW29iSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGdKPTBiPTCwMlU6KM7:TT6=	NHPMXFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS1OFkyQCd-Mkm0OVQ6OTh:L3G+
MDA-MB-435	NEHhXZhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MnK1NlQhcHK\|		NFv3UINCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi12M{WgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDFwN{ig{txONg>?	Mn\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NUS5NVgoRjJ7NEW0PVE5RC:jPh?=
K562	Mn;6SpVv[3Srb36gZZN{[Xl?				MV;Jcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgdIFzfGmjbHz5JJB2emmoaXXkJIhqe3SxbnWg[IVi[2W2eXzhd4Uhd2ZiaIXtZY4hdGW3a3XtbYEhUzV4MjDj[Yxtey5uIFnDOVAhRSByLkCwOFYh\|ryPLh?=	NUnHT4JYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0NlUyOTBpPkGwOFI2OTFyPD;hQi=>
A20	NHrmbFdHfW6ldHnvckBie3OjeR?=				MWLJckB3cXS{bzDmc5Ih[W62aT3ISGFEOSBqSHnzeI9v\SCmZXHj[ZR6dGG\|ZTCxLUBi[3Srdnn0fUBqdiCvb4Xz[UBCOjBiY3XscJMtKEmFNUCgQUAxNjByMjFOwG0v	NHu1NJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUm2NFQ5QSd-MUG5OlA1QDl:L3G+
K 562	MY\GeY5kfGmxbjDhd5NigQ>?				NHzwVnBKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUGm\|dH;u[UBl\WGlZYT5cIF{\SBqSFTBR{khcW5iSzC1OlIh\XK7dHjyc4xmfWunbXnhJINmdGy\|LDDJR\|UxKD1iMD6wNFM1KM7:TT6=	NIX5SXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEW5NlQ4Oyd-MUS1PVI1PzN:L3G+
friend cell	M2TMd2Z2dmO2aX;uJIF{e2G7				MXHJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCocnnlcoQh[2WubIOgdJJwdGmoZYLheIlwdixiSVO1NEA:KDBwMESg{txONg>?	M2W3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NkGzN\|EzLz5zNE[xN\|MyOjxxYU6=
SNU-16	Mm\CSpVv[3Srb36gZZN{[Xl?				NH:ySmpKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUGm\|dH;u[UBl\WGlZYT5cIF{\SBqSFTBR{kh\nKxbTDTUnUuOTZiKHj1cYFvKGejc4TybYMh[WSnbn;jZZJkcW6xbXGpJINmdGy\|LDDJR\|UxKD1iMD6wOVMzQSEQvF2u	M{PlVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2Nk[3NlI4Lz5zNE[2O\|IzPzxxYU6=
melanoma B16/BL6	M1q0bGZ2dmO2aX;uJIF{e2G7				MYLJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbIl{fG:wZTDk[YFk\XS7bHHz[ZMhMEiGQVOxLUBxemWyYYLl[EBnem:vIH3veZNmKG2nbHHuc41iKEJzNj;CUFYh[2WubIOsJGlEPTBiPTCwMlAyQSEQvF2u	M1vkSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MUC5OlI3Lz5zNUGwPVYzPjxxYU6=
melanoma B16/BL6	MVLGeY5kfGmxbjDhd5NigQ>?				M{fvdWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKCiKRFHDOEkheHKncHHy[YQh\nKxbTDtc5V{\SCvZXzhco9u[SCEMU[vRmw3KGOnbHzzMEBKSzVyIE2gNE4xOiEQvF2u	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6	NGjuXFdHfW6ldHnvckBie3OjeR?=				MoTaTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB{ODFOwG0v	NIO1[4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
melanoma B16/BL6	MYDGeY5kfGmxbjDhd5NigQ>?				M2D4R2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKDhicILldIFz\WRiZoLvcUBud3W\|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yNDFOwG0v	MlrNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVzMEm2NlYoRjF3MUC5OlI3RC:jPh?=
293T	NYrJWJFXTnWwY4Tpc44h[XO\|YYm=				NE\ZVVZKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViNDDwdoVx[XKnZDDmdo9uKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCyJO69VS5?	NWL5ZlJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
293T	MYTGeY5kfGmxbjDhd5NigQ>?				M4X2XGlvcGmkaYTpc44hd2ZiaIXtZY4hcGm\|dH;u[UBl\WGlZYT5cIF{\SBzIIDy[ZBiemWmIH\yc40hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>?	NVrLTlNuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
293T	M{HmfmZ2dmO2aX;uJIF{e2G7				M{nlVmlvcGmkaYTpc44hd2ZiaIXtZY4hcGm\|dH;u[UBl\WGlZYT5cIF{\SB4IIDy[ZBiemWmIH\yc40hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>?	M1T2PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEW0NlI1Lz5zNUS1OFIzPDxxYU6=
293T	NVexRXJDTnWwY4Tpc44h[XO\|YYm=				M4XIT2lvcGmkaYTpc44hd2ZiaIXtZY4hcGm\|dH;u[UBl\WGlZYT5cIF{\SB6IIDy[ZBiemWmIH\yc40hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMESg{txONg>?	NUmwT3lWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
HeLa	MUPGeY5kfGmxbjDhd5NigQ>?				Moq5TY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4hcGm\|dH;u[UBl\WGlZYT5cIF{\SBqSFTBR{kh\nKxbTDI[WxiKGOnbHzzJJdqfGhic4Xid5Rz[XSnIEXhMEBKSzVyIE2gNE4xPTR3IN88UU4>	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NkK2O{c,OTV2NU[yOlc9N2F-
HCT116	MXTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				MoX2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDIR3QyOTZiY3XscJMtKEmFNUCgQUAxNjByMUOg{txONg>?	NGXKNGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmwOFg6OCd-MU[5NFQ5QTB:L3G+
H1299	NVX1SHNVSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFixNlk6KGOnbHzzMEBKSzVyIE2gNE4yKM7:TT6=	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlyNEi5NEc,OTZ7MES4PVA9N2F-
H661	MmPNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?				MmXoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDIOlYyKGOnbHzzMEBKSzVyIE2gNE4{KM7:TT6=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlyNEi5NEc,OTZ7MES4PVA9N2F-
H661	NVjXSpFNSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NVu4c2hjPDhiaILz		NHrqZ3ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGg3PjFiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JHhVXCCjc4PhfUwhUUN3MDC9JFAvODh3IN88UU4>	NY\NdGp6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NlQ4PzNpPkG3OlI1Pzd\|PD;hQi=>
WiDr	M3r3cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7				NUjTeJp[UW6qaXLpeIlwdiCxZjDTRXAyOzBibXXkbYF1\WRiY3XscEBoem:5dHigbY4hcHWvYX6gW4lFeiClZXzsd{whUUN3MDC9JFAvPTd5NjFOwG0v	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ2M{GxNkc,OTd4NEOxNVI9N2F-
U251	NVnWXW13TnWwY4Tpc44h[XO\|YYm=				MnnETY5pcWKrdHnvckBw\iCVQWCxN\|AhcW5iVlXHSk1{fGmvdXzheIVlKGi3bXHuJHUzPTFiY3XscJMh[nliUFzBVEBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC54MUGyJO69VS5?	MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NEOxNVIoRjF5NkSzNVEzRC:jPh?=
293T	NYrmVpBqTnWwY4Tpc44h[XO\|YYm=				MnnmTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDlfJBz\XO\|ZXSgbY4hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMECxPUDPxE1w	M1qxPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T	NUeyOJE5TnWwY4Tpc44h[XO\|YYm=				NYTZWIJ1UW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFAzKM7:TT6=	M2nJcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T	M1PPSGZ2dmO2aX;uJIF{e2G7				NGf0bVNKdmirYnn0bY9vKG:oIH3veZNmKEiGQVO2JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNFI5KM7:TT6=	Ml32QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN\|IoRjF5OEixNlMzRC:jPh?=
H661	NVLXVVltSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NITGR2M1QCCqcoO=		Mk\sRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDIOlYyKGOnbHzzJIFnfGW{IES4JIhzeyxiSVO1NEA:KDBwMEi1JO69VS5?	M2rn[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEm3PFI1Lz5zN{i5O\|gzPDxxYU6=
293T	NF[zbFhHfW6ldHnvckBie3OjeR?=				NVjMSZNQUW6qaXLpeIlwdiCxZjDISGFEOSCneIDy[ZN{\WRiaX6gNlk{XCClZXzsd{whUUN3MDC9JFAvOTlizszNMi=>	NHfOSok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
293T	M2CyNGZ2dmO2aX;uJIF{e2G7				M1W3ZWlvcGmkaYTpc44hd2ZiSFTBR\|Qh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlIh\|ryPLh?=	NVu2NXpDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T	M3vZcmZ2dmO2aX;uJIF{e2G7				MoHSTY5pcWKrdHnvckBw\iCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNlgh\|ryPLh?=	NVWx[FZ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
NIH3T3	MYXGeY5kfGmxbjDhd5NigQ>?				NEnMSYFKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDOTWg{XDNiY3XscJMtKEmFNUCgQUAxNjB{MTFOwG0v	MmD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O\|k5RC:jPh?=
human 293	NV3iSWZbTnWwY4Tpc44h[XO\|YYm=				MlPSTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzJINmdGy\|LDDJR\|UxKD1iMD6wN\|Qh\|ryPLh?=	NULKRoF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Nlk4QThpPkG3PVI6Pzl6PD;hQi=>
mouse 293	NYDtN41VTnWwY4Tpc44h[XO\|YYm=				NV2wTZhDUW6qaXLpeIlwdiCxZjDtc5V{\SC{ZXPvcYJqdmGwdDDISGFEPiCneIDy[ZN{\WRiaX6gNlk{KGOnbHzzMEBKSzVyIE2gNE4xQDFizszNMi=>	M{DZNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUK5O\|k5Lz5zN{myPVc6QDxxYU6=
SKBR3	M1ixUWFvfGmycn;sbYZmemG2aY\lJIF{e2G7				NYfhPZBzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT2JTOyClZXzsd{whUUN3MDC9JFAvODJizszNMi=>	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L	NIS0PVlHfW6ldHnvckBie3OjeR?=				MV7Jcohq[mm2aX;uJI9nKGi3bXHuJG1OQT[OIHPlcIx{NCCLQ{WwJF0hOC5yMzFOwG0v	NGjlSVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
SNU16	NXi0bFk{TnWwY4Tpc44h[XO\|YYm=				M1TVXmlvcGmkaYTpc44hd2ZiSFTBR{Bnem:vIHj1cYFvKFOQVUG2JINmdGy\|LDDJR\|UxKD1iMD6wOVMh\|ryPLh?=	NIWwTHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
A549	NH\2dGtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				MkXORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDNUS5JINmdGy\|LDDJR\|UxKD1iMD6wPEDPxE1w	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MKN45	MWfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				NEPBTFZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3LUlQ2KGOnbHzzMEBKSzVyIE2gNE4yKM7:TT6=	NHy5VFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
SQ20B	M4jjfmZ2dmO2aX;uJIF{e2G7				MWjJcohq[mm2aX;uJI9nKGi3bXHuJHNSOjCEIHPlcIx{NCCLQ{WwJF0hOC5{IN88UU4>	M4HRN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
neonatal foreskin fibroblasts	MmLCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?				NWXadZl7T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hdmWxbnH0ZYwh\m:{ZYPrbY4h\mmkcn;icIF{fHNiY3XscJMtKEmFNUCgQUAxNjJizszNMi=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
HeLa	NHWx[ZFHfW6ldHnvckBie3OjeR?=				MoLjTY5pcWKrdHnvckBw\iCKRFHDJIZzd21iaIXtZY4hUGWOYTDj[YxteyxiSVO1NEA:KDBwMzFOwG0v	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L/NFF	NEjPXWpHfW6ldHnvckBie3OjeR?=				NWTMXmRwW2WuZXP0bZZqfHliaX7k[ZgtKHKjdHnvJI9nKEmFNUCg[o9zKGi3bXHuJG1OQT[OIHPlcIx{KHSxIFnDOVAh\m:{IHj1cYFvKE6IRjDj[YxteyxiSVO1NEA:KDZwNzFOwG0v	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
293T	MoflSpVv[3Srb36gZZN{[Xl?				M3WwTmlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Gg[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCzOlYh\|ryPLh?=	M3HuclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{m3PFI3Lz5zOEO5O\|gzPjxxYU6=
293T	MW\GeY5kfGmxbjDhd5NigQ>?				M1juOmlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Sg[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkC2Nlkh\|ryPLh?=	NGfxVWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O\|gzPid-MUizPVc5OjZ:L3G+
293T	NXfEenR3TnWwY4Tpc44h[XO\|YYm=				NYXCRlZJUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFg{OyEQvF2u	NE\DO5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O\|gzPid-MUizPVc5OjZ:L3G+
HeLa	NHTTR\|NHfW6ldHnvckBie3OjeR?=				MnnQTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFIHnuJGhmVGFiY3XscJMh[nliZnzveZIh\GVibInzJIF{e2G7LDDJR\|UxKD1iMD6wNFUh\|ryPLh?=	NXPYcHlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVc5OjdpPkG4N\|k4QDJ5PD;hQi=>
S2	MUTGeY5kfGmxbjDhd5NigQ>?				MkLPTY5pcWKrdHnvckBw\iCSbHHzcY9lcXWvIH\hcINqeGG{dX2gTGRCSzFiZYjwdoV{e2WmIHnuJGRzd3OxcHjpcIEhdWWuYX7v[4F{fGW{IGOyJINmdGy\|LDDJR\|UxKD1iMD6wNFA3KM7:TT6=	NETzZY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOxO\|Q2OCd-MUmzNVc1PTB:L3G+
NCI-H661	NXHjNodsSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MkLrOFghcHK\|		NFrKcllCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTU1JPjZzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjFizszNMi=>	MljyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|OEW2NFAoRjF7M{i1OlAxRC:jPh?=
NIH3T3	MoLGSpVv[3Srb36gZZN{[Xl?				M4jJemlvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hVkmKM2SzJINmdGy\|IHL5JJJi\GmxbXX0dolkKGirc4TvcoUh\GWjY3X0fYxifGmxbjDhd5NigSxiSVO1NEA:KDBwMEC2JO69VS5?	M4Kz[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEW3OlU6Lz5zOUS1O\|Y2QTxxYU6=
HeLa	MkPmSpVv[3Srb36gZZN{[Xl?				MXfJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBJ\UyjIHPlcIx{KGK7IH\seY9zd2enbnnjJIVvgnmvZTDhd5NigSxiSVO1NEA:KDBwMzFOwG0v	NXmyXphsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVc3PTlpPkG5OFU4PjV7PD;hQi=>
ML1	NGP3RnVCdnSrdIXtc5Ih[XO\|YYm=				NVm0OHA4SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhdW:3c3WgUWwyKGOnbHzzMEBKSzVyIE2gNE4zPSEQvF2u	NIfaWFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWzOFU{PCd-MUm1N\|Q2OzR:L3G+
HCT116	NInjZlJEgXSxdH;4bYNqfHliYYPzZZk>		NVTmOplXOyCmYYnz		NF7aWpJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KFeVVD2xJIF{e2G7LDDJR\|UxKD1iMD64JO69VS5?	NFjvOFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPid-MkC0OVIzOjZ:L3G+
HCT116	NHvpd2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NYXSWFlzPDhiaILz		MYrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvODR\|IN88UU4>	NEHRbI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC3N\|E3OCd-MkGwO\|MyPjB:L3G+
H460	Mk\lS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MlPyOFghcHK\|		NX;E[XF4T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUDR4MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5zIN88UU4>	NIT0cW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC3N\|E3OCd-MkGwO\|MyPjB:L3G+
DMS53	MmPDR5l1d3SxeHnjbZR6KGG\|c3H5		NIHOdoszKGSjeYO=		NX3DXJh3S3m2b4P0ZZRq[yCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGROWzV\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB{NTFOwG0v	NIfnVGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWwOFIyPCd-MkG1NFQzOTR:L3G+
HeLa	NX7nRZhuTnWwY4Tpc44h[XO\|YYm=		M4DNVFIxKG2rboO=		NHjP[HFKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDj[YxteyC3c3nu[{BC[y2DcnetS4x6NUy7czjBZ{kuSU2FIHHzJIZtfW:{ZYPj[Y51KHO3YoP0doF1\SCjZoTldkAzOCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMECzJO69VS5?	Mli2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4MkG4PFMoRjJzNkKxPFg{RC:jPh?=
Sf9	MU\GeY5kfGmxbjDhd5NigQ>?		NF7mWlAyKGi{		MYLJcohq[mm2aX;uJI9nKE5idHXycYlv[WxiaHX4ZYhqe3SrZHnu[U11[WepZXSgbJVu[W5iSFTBR\|gh\XiycnXzd4VlKGmwIGPmPUBk\WyuczDh[pRmeiBzIHjyJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwOU[g{txONg>?	NGrueGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUeyN\|c{Oyd-MkG3NlM4OzN:L3G+
MCF7	M{nCcWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NYrJdHpIPDhiaILz		NHvGVGNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuO{DPxE1w	Mnv1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkWwPVEoRjJ{NE[1NFkyRC:jPh?=
BE(2)-C	NWq3bHluSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MVy3NkBpenN?		Mm\pRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCERTiyLU1EKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDBcIFu[XJiYnz1[UBie3OjeTygTWM2OCB;IECuNFUh\|ryPLh?=	M2\IWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUOyN\|E3Lz5{MkmzNlMyPjxxYU6=
Sf9	MnP0SpVv[3Srb36gZZN{[Xl?		Ml;kNkBpenN?		MYXJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JIV5eHKnc4Pl[EBqdiCVZkmgZ4VtdHNiaX7jeYJifGWmIH\vdkAzKGi{czD1d4lv\yCUSFvLMWFkKG[udX;yc4dmdmmlIIP1ZpN1emG2ZTygTWM2OCB;IECuNFAyOiEQvF2u	M4LzRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEC5NlA{Lz5{M{CwPVIxOzxxYU6=
Sf9	MXnGeY5kfGmxbjDhd5NigQ>?		MWKyJIhzew>?		NH;MZ\|VKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgbY5kfWKjdHXkJIZweiB{IHjyd{B2e2mwZzDSTGtMNUGlIH\seY9zd2enbnnjJJN2[nO2cnH0[UwhUUN3MDC9JFAvODB3IN88UU4>	NXTjUmt6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFkzODNpPkKzNFA6OjB\|PD;hQi=>
A2780	MWrDfZRwfG:6aXPpeJkh[XO\|YYm=		M4nDN\|czKGi{cx?=		MkjmR5l1d3SxeHnjbZR6KGGpYXnud5Qh[2m\|cHzheIlvKHKnc3nzeIFvfCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{MjFOwG0v	NH\jWVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
A2780	MXHGeY5kfGmxbjDhd5NigQ>?		NFW2VI0yQCCqcoO=		Mof4TY5pcWKrdHnvckBw\iCKRFHDJIlvKGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMkWg{txONg>?	MnO3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ\|MkWyOlA{RC:jPh?=
A2780	NHTNV3NEgXSxdH;4bYNqfHliYYPzZZk>		MX:3NkBpenN?		M4fybWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ7IN88UU4>	NYTDPYNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB\|PD;hQi=>
A2780	MXvGeY5kfGmxbjDhd5NigQ>?		NIHBVGIyQCCqcoO=		MmXCTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFE5KGi{czDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjR\|IN88UU4>	MmDGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ\|MkWyOlA{RC:jPh?=
Sf9	MlviSpVv[3Srb36gZZN{[Xl?				MmW2TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOkBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOvd4Y6KGOnbHzzJJV{cW6pIGLIT2tC[yCjczDzeYJ{fHKjdHWsJGlEPTBiPTCwMlAxODlizszNMi=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
Sf9	NILXNFBHfW6ldHnvckBie3OjeR?=				NYXBXHhKUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMwe2Z7IHPlcIx{KHW\|aX7nJHJJU0uDYzDhd{B{fWK\|dILheIUtKEmFNUCgQUAxNjByNTFOwG0v	M{DCOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
Sf9	Mn\uSpVv[3Srb36gZZN{[Xl?				M1\D[mlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR\|Ih\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzM5NnQSClZXzsd{B2e2mwZzDSTGtMSWNiYYOgd5Vje3S{YYTlMEBKSzVyIE2gNE4xODVizszNMi=>	NFj6cFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
Sf9	NInqSohHfW6ldHnvckBie3OjeR?=				MknsTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDNVAh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzM5NnQSClZXzsd{B2e2mwZzDSTGtMSWNiYYOgd5Vje3S{YYTlMEBKSzVyIE2gNE4xODlizszNMi=>	NWrlOm5HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
Sf9	MXnGeY5kfGmxbjDhd5NigQ>?				MXvJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxNUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOvd4Y6KGOnbHzzJJV{cW6pIGLIT2tC[yCjczDzeYJ{fHKjdHWsJGlEPTBiPTCwMlAyPzNizszNMi=>	MkK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ\|OUC1OlgxRC:jPh?=
Sf9	M3HpNmZ2dmO2aX;uJIF{e2G7				MnP1TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDPEBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOvd4Y6KGOnbHzzJJV{cW6pIGLIT2FkU0GlIHHzJJN2[nO2cnH0[UwhUUN3MDC9JFAvOTJ7IN88UU4>	M3HwRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
MOLT4	MUjDfZRwfG:6aXPpeJkh[XO\|YYm=		MVy3NkBpenN?		MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkCzJO69VS5?	NGnIb3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
A549	M1e4WWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NFTUS2U4OiCqcoO=		MlLNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkC1JO69VS5?	NU[5OVdzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
MCF7	NYPxendmS3m2b4TvfIlkcXS7IHHzd4F6		NYrzR5JlPzJiaILz		NFLzWJhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuNFYh\|ryPLh?=	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4ME[yO{c,OjR7NkC2Nlc9N2F-
HL60	NXHxW49CS3m2b4TvfIlkcXS7IHHzd4F6		MVi3NkBpenN?		M3O1SmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFQ1u4JIF{e2G7LDDJR\|UxKD1iMD6wPEDPxE1w	NGD6eWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
BGC823	M2[0[WN6fG:2b4jpZ4l1gSCjc4PhfS=>		MVW3NkBpenN?		Ml\GR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuNFgh\|ryPLh?=	Mlm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
HuH7	NIDYeFNEgXSxdH;4bYNqfHliYYPzZZk>		NYnDRmMyPzJiaILz		MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhS0ONODDhd5NigSxiSVO1NEA:KDBwMTFOwG0v	M1PhWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wOlI4Lz5{NEm2NFYzPzxxYU6=
U937	M4HnWmN6fG:2b4jpZ4l1gSCjc4PhfS=>		M33DWFczKGi{cx?=		MmLPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkGg{txONg>?	NXHEbnhsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
HeLa	MlXLR5l1d3SxeHnjbZR6KGG\|c3H5		M4Xk[\|czKGi{cx?=		MoPlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkGxJO69VS5?	NFP2PW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
NCI-H1975	MmrOR5l1d3SxeHnjbZR6KGG\|c3H5		NYTCbZZYPzJiaILz		NEThW4ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSEG5O\|Uh[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBES0t6IHHzd4F6NCCLQ{WwJF0hOC5{MTFOwG0v	Mn\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
K562	M{LFXmN6fG:2b4jpZ4l1gSCjc4PhfS=>		NU\VWFJiPzJiaILz		NIXZcINEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuOFEh\|ryPLh?=	NWP2Ooo6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
HEK293	MX7DfZRwfG:6aXPpeJkh[XO\|YYm=		M37iS\|k3KGi{cx?=		NUXGd5FuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEWNMkmzJINmdGy\|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkG2JO69VS5?	MnHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NU[xNFIoRjJ3NUW2NVAzRC:jPh?=
LO2	MXzDfZRwfG:6aXPpeJkh[XO\|YYm=		NVy3VoE2QTZiaILz		M1exN2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxQOiClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNE4zOSEQvF2u	M3m2eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW2NVAzLz5{NUW1OlExOjxxYU6=
HeLa	NIr1dWNEgXSxdH;4bYNqfHliYYPzZZk>		MUW5OkBpenN?		MkHNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNE45PSEQvF2u	MoHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NU[xNFIoRjJ3NUW2NVAzRC:jPh?=
HepG2	M2[5cWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MX:5OkBpenN?		MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNE46PiEQvF2u	NI\SXYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OlExOid-MkW1OVYyODJ:L3G+
U2OS	NYfQSodES3m2b4TvfIlkcXS7IHHzd4F6		NVnJXFhqQTZiaILz		M{XiR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHUzV1NiY3XscJMh[W[2ZYKgPVYhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFE1Njl\|IN88UU4>	MmnaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NU[xNFIoRjJ3NUW2NVAzRC:jPh?=
Sf9	MV\GeY5kfGmxbjDhd5NigQ>?		NFvVSnI6OCCvaX7z		MV\Jcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IF6teIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFNjCoNUB1dyBzMkG1JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5ic3[5JINmdGy\|IIXzbY5oKFKKSz3LLGFkMS2DTVOgZZMhe3Wkc4TyZZRmKGmwY4XiZZRm\CCob4KgPVAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjByMUexOkDPxE1w	M1LGWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO\|Y1Lz5{N{C2NFc3PDxxYU6=
Sf21	NIryc4NHfW6ldHnvckBie3OjeR?=		M2Lt[FYxKG2rboO=		MoPoTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGZNSUdvSHnzMZRi\2enZDDISGFEOSBqMTD0c{A1QDJicnXzbYR2\XNrIHX4dJJme3OnZDDpckBU\jJzIHPlcIx{KHW\|aX7nJHJJUy2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIlv[3WkYYTl[EBnd3JiNkCgcYlveyCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTBiPTCwMlAxPjV5NDFOwG0v	NY\ZNY5XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
insect cell	M2nte2Z2dmO2aX;uJIF{e2G7		MYO2NEBucW6\|		NEXEN3pKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIILlZ49u[mmwYX70JGMufGW{bXnuZYwhT1OWLYTh[4dm\CCKRFHDNkApOSC2bzC0PFghemW\|aXT1[ZMqKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMhfXOrbnegVmhMNUtqQXOpMWFOSyCjczDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB4MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFExPTFizszNMi=>	NWXDPHozRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
insect cell	NWHHb5NNTnWwY4Tpc44h[XO\|YYm=		MYe2NEBucW6\|		MoDHTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{OgLFEhfG9iNEK4JJJme2mmdXXzLU9pfW2jbjDy[YNwdWKrbnHueEBPNXSncn3pcoFtKEeVVD30ZYdo\WRiTlPPVlIh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{B2e2mwZzDSTGsuUyiDYzmtRW1EKGG\|IIP1ZpN1emG2ZTDpcoN2[mG2ZXSg[o9zKDZyIH3pcpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxKD1iMD6wNVExPyEQvF2u	NVHvcYVzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
insect cell	NGLqOW9HfW6ldHnvckBie3OjeR?=				NHfZV4RKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IF6teIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFMUGgLFEhfG9iM{S3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzM4Ji[3Wub4\pdpV{KGW6cILld5Nqd25ic4nzeIVuKHW\|aX7nJHJJUy2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMEGxN\|ch\|ryPLh?=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
insect cell	MUfGeY5kfGmxbjDhd5NigQ>?		NWnOUmF2PjBibXnudy=>		NYjXUVVZUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{igLFEhfG9iM{e3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3LLGFkMS2DTVOgZZMhe3Wkc4TyZZRmKGmwY4XiZZRm\CCob4KgOlAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjNyN{Sg{txONg>?	MnnIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO\|Y1RC:jPh?=
SHSY5Y	Ml:ySpVv[3Srb36gZZN{[Xl?		M1y5fVghcHK\|		M3fjWGlvcGmkaYTpc44hd2ZiSFTBR\|YhcW5iaIXtZY4hW0iVWUXZJINmdGy\|IIXzbY5oKEKDVFPQJIF{KHO3YoP0doF1\SCjZoTldkA5KGi{czDifUBWUFCOQz3FV2kuVVNxTWOgZY5idHm\|aYOsJGlEPTBiPTCwMlAxPzhizszNMi=>	NILwbWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[1NFkzPSd-Mke2OVA6OjV:L3G+
SHSY5Y	MkXlSpVv[3Srb36gZZN{[Xl?		Mnf1PEBpenN?		NUDlPY1pUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKFOKU2m1XUBk\WyuczD1d4lv\yCPQVygZZMhe3Wkc4TyZZRmKGGodHXyJFghcHK\|IHL5JHVJWEyFLVXTTU1OWy:PUzDhcoFtgXOrczygTWM2OCB;IECuNFA6OSEQvF2u	NV3zPGxIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
Sf9	NGfURlNHfW6ldHnvckBie3OjeR?=		M1LXV\|QhcHK\|		NV3o[Fc3UW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDHV3QufGGpZ3XkJGhFSUNzIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCVZkmgbY5{\WO2IHPlcIx{KHW\|aX7nJG1QS1CDQzDhd{B{fWK\|dILheIUh[W[2ZYKgOEBpenNiYomgWWhRVENvRWPJMW1UN02VIHHuZYx6e2m\|LDDJR\|UxKD1iMD6wNVU4KM7:TT6=	NX\zcVBURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
insect cell	MoG1SpVv[3Srb36gZZN{[Xl?		M2Pm[VQhcHK\|		MWPJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDpcpNm[3RiY3XscJMhfXOrbnegRmFVS1BiYYOgd5Vje3S{YYTlJIFnfGW{IESgbJJ{KGK7IGXIVGxENUWVST3NV{9OWyCjbnHsfZNqeyxiSVO1NEA:KDBwMEG2JO69VS5?	MlOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4NUC5NlUoRjJ5NkWwPVI2RC:jPh?=
SHSY5Y	MnSwSpVv[3Srb36gZZN{[Xl?		MnHDPEBpenN?		M17WfGlvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hW0iVWUXZJINmdGy\|IIXzbY5oKE2RQ2DBR{BieyC\|dXLzeJJifGViYX\0[ZIhQCCqcoOgZpkhXUiSTFOtSXNKNU2VL13TJIFv[Wy7c3nzMEBKSzVyIE2gNE4xOTh6IN88UU4>	M4rFfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkWwPVI2Lz5{N{[1NFkzPTxxYU6=
HEK293T	M4rxcGZ2dmO2aX;uJIF{e2G7		M4j1[VMxKG2rboO=		NHjuS5FKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFjERWMyKGW6cILld5Nm\CCrbjDISWszQTOWIHPlcIx{KHW\|aX7nJGFkNUuJTFfLLGFkMS2PQ1GgZZMhe3Wkc4TyZZRmKGGodHXyJFMxKG2rboOgbY4heHKnc3XuZ4Uhd2ZiRGTUJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMEG5JO69VS5?	M2fvdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUG5OlA1Lz5{OUWxPVYxPDxxYU6=
HEK293T	M1PX[GZ2dmO2aX;uJIF{e2G7		NI\aWVA{OCCvaX7z		MUPJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IH3veZNmKEiGQVO2JIV5eHKnc4Pl[EBqdiCKRVuyPVNVKGOnbHzzJINwNWW6cILld5NqdmdibX;1d4UhUESDMjD1d4lv\yCDYz3LS2xIUyiDYzmtUWNCKGG\|IIP1ZpN1emG2ZTDh[pRmeiB\|MDDtbY5{KGmwIIDy[ZNmdmOnIH;mJGRVXCCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTBiPTCwMlAzQCEQvF2u	NELDR4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxPVYxPCd-Mkm1NVk3ODR:L3G+
NFF	MVTDfZRwfG:6aXPpeJkh[XO\|YYm=				Mk\ZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUmZHKGOnbHzzMEBKSzVyIE2gNE4zKM7:TT6=	NFrMXXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK0OVQxOid-M{CyOFU1ODJ:L3G+
insect cell	MnLaSpVv[3Srb36gZZN{[Xl?				NUO0d3JbUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{[g[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD6wNFg2KM7:TT6=	NYTjUWFMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN\|Y2QDl{PD;hQi=>
Sf21	NGrVNGpHfW6ldHnvckBie3OjeR?=				NXfZd\|MyUW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGZNSUdvSHnzMZRi\2enZDDmeYxtKGynbnf0bEBz\WOxbXLpcoFvfCCqdX3hckBJTEGFMTDlfJBz\XO\|ZXSgbY4hW2Z{MTDpcpNm[3RiY3XscJMhfXOrbnegVmhMUyiDYznBUWMh[XNic4Xid5Rz[XSnIHL5JIZtfW:{ZYPj[Y5k\S2kYYPl[EBie3OjeTygTWM2OCB;IECuNFA6PCEQvF2u	NFr1TFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEO2OVg6Oid-M{CzOlU5QTJ:L3G+
insect cell	NUnvVnR4TnWwY4Tpc44h[XO\|YYm=				M{WzTmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iSFTBR\|MwVkORUkKg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD6wNVI4KM7:TT6=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB\|NkW4PVI9N2F-
insect cell	NHjJNZNHfW6ldHnvckBie3OjeR?=				NVrW[GtNUW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{Kg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD6wNlY4KM7:TT6=	M3zXOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{[1PFkzLz5\|MEO2OVg6OjxxYU6=
insect cell	MXjGeY5kfGmxbjDhd5NigQ>?				NFLyUVZKdmirYnn0bY9vKG:oIFOteIVzdWmwYXygTIl{NXSjZ3fl[EBz\WOxbXLpcoFvfCCqdX3hckBJTEGFODDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemW\|Y3XuZ4Uu[mG\|ZXSgZZN{[XluIFnDOVAhRSByLk[wPUDPxE1w	NUjqPIt[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN\|Y2QDl{PD;hQi=>
A20	NUnvWoFrTnWwY4Tpc44h[XO\|YYm=				NGGxdpZDcW6maX7nJIFn\mmwaYT5JJRwf2G{ZIOgTGRCSzFiKFjpd5RwdmViZHXhZ4V1gWyjc3WgNUkhcW5ibX;1d4UhSTJyIHPlcIx{NCCneIDy[ZN{\WRiYYOgZolv\GmwZzDjc45{fGGwdDCodGtqMSxiS3mgQUAxNjByMUC3NkDPxE1w	NXHMdYlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5OlA1QDlpPkGxPVYxPDh7PD;hQi=>
Hela	NFT5SZJHfW6ldHnvckBie3OjeR?=				MWHJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVt[SClZXzsd{BvfWOuZXHyJIV5fHKjY4TzJIJ6KG[udX;ybY1mfHKrYzDhd5NigSxiS3mgQUAxNjVyNDFOwG0v	NIHwT\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWyNFU5OCd-MUm1NlA2QDB:L3G+
Sf9	NELkZXNHfW6ldHnvckBie3OjeR?=		Mo\TPVAhdWmwcx?=		MYXJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIF6teIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFNjDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiU3[5JIlve2WldDDj[YxteyC3c3nu[{BTUEuNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiOUCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlAxODV6IN88UU4>	NELac4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
Sf21	M2\EdmZ2dmO2aX;uJIF{e2G7		MnfQOlAhdWmwcx?=		MV;Jcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKENvdHXycYlv[WxiRlzBS{9JcXNvdHHn[4VlKEiGQVOxJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3cz3pcoZm[3SnZDDT[lIyKGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK\|dILheIUh[W[2ZYKgOlAhdWmwczDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjByME[0JO69VS5?	NF35Uow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell	MoWySpVv[3Srb36gZZN{[Xl?		M1XxelYxKG2rboO=		NGHUeoZKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJJJm[2:vYnnuZY51KGi3bXHuJGMufGW{bXnuZYwhUGm\|LYTh[4dm\CCKRFHDN{9PNXSncn3pcoFtKEeVVD30ZYdo\WRicnXjc41jcW6jboSgbJVu[W5iTlPPVlIhMDN7NTD0c{A1QDlicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdiUljLT{hC[ymDTVOgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMEC4OEDPxE1w	M1flbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell	MVnGeY5kfGmxbjDhd5NigQ>?		NFTSbJI3OCCvaX7z		MWLJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIFOteIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFMjDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KG[udX;ybY1mfGW{LDDLbUA:KDBwMECzOVUh\|ryPLh?=	NFfOR\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell	NV7He4lrTnWwY4Tpc44h[XO\|YYm=				NV34cYtbUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDISGFEOTBiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCobIXvdo9o\W6rYzDw[ZB1cWSnIGLIT2tC[yCjczDzeYJ{fHKjdHWgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMES3OkDPxE1w	M2TTNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell	M3vRdmZ2dmO2aX;uJIF{e2G7		M1S0OVYxKG2rboO=		NUfUZWNoUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDDMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{igLFEhfG9iM{e3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOtbY5n\WO2ZXSgbY5{\WO2IHPlcIx{KHW\|aX7nJHJJUyiDYznLLGFkMUGPQzDhd{B{fWK\|dILheIUh[W[2ZYKgOlAhdWmwczDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjF2NTFOwG0v	NVfkVGpnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell	M135R2Z2dmO2aX;uJIF{e2G7				MUTJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE5vdHXycYlv[WxiSHnzMZRi\2enZDDISGFEOTFiKEGgeI8hOzR5IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemmvZYTldkwhU2liPTCwMlQxOSEQvF2u	NFjkTpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
GM15850	MnXhSpVv[3Srb36gZZN{[Xl?	MUGwMlEhfU1?	NFLucIwyOiCqcoO=		NW\sPWtiUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEePMUW4OVAh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5idH;0ZYwh[WOndInsZZRm\CCqaYP0c45mKGyndnXsJIF1KDBwMTD1UUBi\nSncjCxNkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
A549	NF\5SJhHfW6ldHnvckBie3OjeR?=	M1W4T\|AvOSC3TR?=			NIDIPHNKdmirYnn0bY9vKG:oIGPJVnQzKGmwIFG1OFkh[2WubIOgZZN{\XO\|ZXSgZZMh[WKrbHn0fUB1dyCrbnT1Z4UhcHmyZYLhZ4V1gWyjdHnvckBw\iC2dXL1cIlvKGK7IGfld5Rmem5iYnzveEBidmGueYPpd{BifCByLkGgeW0>	NEDpRXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G0PVg3OCd-MUexOFk5PjB:L3G+
ST14A	NVzEXZVLTnWwY4Tpc44h[XO\|YYm=	NUnVNYt6PzVibl2=			NWTmSGd[UW6mdXP0bY9vKG:oIGLFV3QwVlKVRj3jc5VxdGWmIILldJJme3Orb36gc4YhdHWlaX\ldoF{\SC2YXfn[YQhemG2IGLFNU9PWlOHIFLEUmYheHKxbX;0[ZIh[WO2aY\peJkh\XiycnXzd4VlKGmwIGPUNVRCKGOnbHzzJIF1KDd3IH7NJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHz1Z4ln\XKjc3WgZYN1cX[rdIm=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4NUm5N{c,OTd3NkW5PVM9N2F-
HEK293	NFjpd4tHfW6ldHnvckBie3OjeR?=	NWniTmMxPjByIH7N			MVPJcoNz\WG\|ZTDpckBIWlB5ODDwdo91\WmwIHzleoVtKGmwIFjFT\|I6OyClZXzsd{BifCB4MECgcm0h[nliaX3teY5w[myxdB?=	NUjoO5NmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlY4OzJpPkG3OVY3PzN{PD;hQi=>
HEK293	MoDjSpVv[3Srb36gZZN{[Xl?	NIj6eFQ3ODBibl2=			M1zobmlv[3KnYYPlJIlvKHC{b4TlbY4h\Gm\|dXzmbYRmKGm\|b33ldoF{\SCuZY\lcEBqdiCKRVuyPVMh[2WubIOgZZQhPjByIH7NJIJ6KGmvbYXuc4Jtd3R?	NF7LRpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2Olc{Oid-MUe1OlY4OzJ:L3G+
HeLa	NUjuflU{TnWwY4Tpc44h[XO\|YYm=	MYexJJVO	M2PmXlI1KGi{cx?=		MX7JcoR2[3Srb36gc4YheDF4IHX4dJJme3Orb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6p	Mmm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NEOxNVEoRjF5NkSzNVEyRC:jPh?=
HeLa	Ml\kSpVv[3Srb36gZZN{[Xl?	M3O3d\|EhfU1?	MUKyOEBpenN?		Moe2TY5lfWO2aX;uJI9nKHB{NzDlfJBz\XO\|aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91fGmwZx?=	NVO5PGZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OFMyOTFpPkG3OlQ{OTFzPD;hQi=>
HeLa	MmrxSpVv[3Srb36gZZN{[Xl?	M3TNcFEhfU1?	NYeybmJEOjRiaILz		Mn\ZTY5lfWO2aX;uJI9nKHB{MTDlfJBz\XO\|aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91fGmwZx?=	NG\sRW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[0N\|EyOSd-MUe2OFMyOTF:L3G+
HCT116	NUXlbXprTnWwY4Tpc44h[XO\|YYm=				Mkj1TY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDhd5Nme3OnZDDhd{BpcXO2b37lJIh6eGW{YXPleJlt[XSrb36gbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGK7IGfld5Rmem5iYnzveC=>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB3NEKzPUc,OThyNUSyN\|k9N2F-
HCT116	MmrZSpVv[3Srb36gZZN{[Xl?				NVizSIdbUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOkBie3Onc4Pl[EBieyC2dXL1cIlvKGi7cHXyZYNmfHmuYYTpc44hcW5iaIXtZY4hUEOWMUG2JINmdGy\|IHL5JHdme3Sncn6gZoxwfA>?	M1HZZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEW0NlM6Lz5zOEC1OFI{QTxxYU6=
HEK293 STF	M3vVcGZ2dmO2aX;uJIF{e2G7		MWGyOEBpenN?		NVvveHlnSWO2aY\heIlwdiCxZjDtc5V{\SCZboSzZUB{cWewYXzpcoch\XiycnXzd4VlKGmwIFjFT\|I6OyCVVF[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmVvYnHz[YQhdGGldHH0[UBl\Wi7ZILv[4Vv[XOnIHHzd4F6	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB{Mk[3OUc,OTlyMkK2O\|U9N2F-
NCI-H661	NHzIPYtHfW6ldHnvckBie3OjeR?=	NUPpZ\|d[OzByIH7N	M3XG[\|YhcHK\|		MUDJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gUmNKNUh4NkGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKGirc4TvcoUhUDRiYXPleJlt[XSrb36gZZQhOzByIH7NJIFnfGW{IE[gbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	M1fDRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{i1OlAxLz5zOUO4OVYxODxxYU6=
NCI-H661	NFq4[XVHfW6ldHnvckBie3OjeR?=	NEHiXFU{ODBibl2=	M13KXFYhcHK\|		NHHoXFVKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hVkOLLVi2OlEh[2WubIOgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIHHsdIhiNXS3YoXsbY4h[WOndInsZZRqd25iYYSgN\|AxKG6PIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?=	NIfEU4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO4OVYxOCd-MUmzPFU3ODB:L3G+
HL60	MXvGeY5kfGmxbjDhd5NigQ>?	MVeyNEB2\y:vbB?=	M3rqelI1KGi{cx?=		MkSzTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhNPjBiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdiCjdDCyNEB2\y:vbDDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	M2PlbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUO0OFcyLz5zOUWzOFQ4OTxxYU6=
primary bronchial epithelial cell	MoT3SpVv[3Srb36gZZN{[Xl?	M1;LOVAvOSC3TR?=			M4iybGlvcGmkaYTpc44hd2ZiSFTBR\|chcW5iaIXtZY4heHKrbXHyfUBjem:wY3jpZYwh\XCrdHjlcIlidCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gcZV1[W62IF\k[Yx1[TVyODDDSnRTKHC{b4TlbY4h[XCrY3HsJJN2em[jY3WgcI9k[WyrenH0bY9vKGG2IECuNUB2VQ>?	NITrbG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
MCF7	MVPGeY5kfGmxbjDhd5NigQ>?	NXu0WppPOC5zIIXN	NHGx[pcyPiCqcoO=		Ml;mTY5pcWKrdHnvckBw\iCVSWLUNkBqdiCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IIT1ZpVtcW5iaInw[ZJi[2W2eXzheIlwdiCjdDCwMlEhfU1iYX\0[ZIhOTZiaILzJIJ6KFenc4Tldo4h[myxdB?=	NImwUpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MECzNFM1Oyd-MkCwN\|A{PDN:L3G+
HeLa	NH;T[HJHfW6ldHnvckBie3OjeR?=	NVPlRpJsOSC3TR?=	NGXrOmEyOCCvaX7z		NFzxbW5KdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxJJVOKGGodHXyJFExKG2rboOgZpkh\my3b4LvcYV1emmlIHHzd4F6	Moi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyNUW4OFgoRjJzMEW1PFQ5RC:jPh?=
MCF7	M4jnSWZ2dmO2aX;uJIF{e2G7	MmHhNE42KHWP	NIHPS28zPCCqcoO=		M1rMeGlvcGmkaYTpc44hd2ZiQ3zhd5MhOSCqaYP0c45mKGSnYXPleJlt[XOnIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhfXBvcnXneYxifGmxbjDv[kBxOjFicILveIVqdiCuZY\lcEBifCByLkWgeW0h[W[2ZYKgNlQhcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>?	MoPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN\|Y{RC:jPh?=
MCF7	NYLoUpAyTnWwY4Tpc44h[XO\|YYm=	M364VlAvPSC3TR?=	MXOyOEBpenN?		NHX6U3lKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgZ4hidmenIHnuJJR2[nWuaX6gZYNmfHmuYYTpc44hdGW4ZXygZZQhOC53IIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
MCF7	MoHGSpVv[3Srb36gZZN{[Xl?	M1jzRlAvPSC3TR?=	NU\jVpNQOjRiaILz		MnrHTY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IIXwMZJm\3WuYYTpc44hd2ZiYXPleJlt[XSnZDDobZN1d26nIFizJJBzd3SnaX6gcIV3\WxiYYSgNE42KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	Mm\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN\|Y{RC:jPh?=
PC12	M1nReWZ2dmO2aX;uJIF{e2G7	NUTufnpTOTBidV2=	NIDnVVI1QCCqcoO=		NWjGcnlbUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDhZ4V1gWyjdHnvckBw\iCqaYP0c45mKEh\|IHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
PC12	MWnGeY5kfGmxbjDhd5NigQ>?	MorYNVAhfU1?	MXi0PEBpenN?		MXfJcohq[mm2aX;uJI9nKEiGQVO2JIlvKHKjdDDQR\|EzKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:wIHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{	MoDPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ\|OUC1OlgxRC:jPh?=
PC12	NF64d\|JHfW6ldHnvckBie3OjeR?=	NUnXeG9kOTBidV2=	NUXafmVWPSCmYYnz		NEnxdlNKdmS3Y4Tpc44hd2ZibnX1dol1\SCxdYTndo94fGhiaX6gdoF1KFCFMUKgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhPSCmYYnz	MnnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ\|OUC1OlgxRC:jPh?=
SH-SY5Y	MkG3SpVv[3Srb36gZZN{[Xl?	M3HsOVExKHWP	MV:1JIRigXN?		MkS2TY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{	M1X6NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
A549	M{O5UGFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NXXLUIdnOC52IIXN	M4m4dFI1KGi{cx?=		NEjnbZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO\|ZXSgZZMhe2i{dX7r[Y4h[W6mIIPoZZJxKHOqYYDlJIF1KDBwNDD1UUBi\nSncjCyOEBpenNiYomgdIhie2ViY3;ueJJie3RibXnjdo9{[2:yeR?=	M3\LclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi0NVEzLz5{NUi4OFEyOjxxYU6=
HeLa	NEX0bY9HfW6ldHnvckBie3OjeR?=				NIjCRlFKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgeJVjfWyrbjDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IH;2[ZJvcWeqdDDifUBqdW23bn;mcJVwemW\|Y3XuZ4UhdWmlcn;zZ49xcWNiYX7hcJl{cXN?	NE\LXmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[xNVkyQSd-Mk[2NVE6OTl:L3G+
C127-LT	M3G0emZ2dmO2aX;uJIF{e2G7	M3jvZVIxOCCwTR?=	NUnpdppsOjRiaILz		MYDJcohq[mm2aX;uJI9nKEiGQVOgbY4hdW:3c3WgR\|EzPy2OVDDj[YxteyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKEWJRmCg[ZhxemW\|c3nvckBifCB{MECgcm0h[W[2ZYKgNlQhcHK\|IHL5JIZtfW:{ZYPj[Y5k\SCvaXPyc5Nkd3C7	M2TDWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm2N\|czLz5{Nkm5OlM4OjxxYU6=
HeLa nuclear	NEe5PXNHfW6ldHnvckBie3OjeR?=	NYjz[JFjOTBidH:gNlUhfU1?	M{fiUVMxKG2rboO=		NX7qWY9vS2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBJTEGFIHnuJIh2dWGwIFjlUIEhdnWlbHXhdkBk\WyuczDheEAyOCC2bzCyOUB2VSC3c3nu[{BDd2NvYXPleJltNWy7c3nu[U1CVUNiYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiYomgUIlv\XenYY\ldk1DfXKtIIDsc5Qh[W6jbInzbZM>	NGDTdlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa	Ml\mSpVv[3Srb36gZZN{[Xl?	MofONkB2VQ>?	M4nFPFI1KGi{cx?=		Mn2xTY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHj5dIVz[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCB{IIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
HN13	MYnGeY5kfGmxbjDhd5NigQ>?		MWWyOEBpenN?		M336[GlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJVjF\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBpcXO2b37lJGg{KEy7c{mgZYNmfHmuYYTpc44hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJIF{e2G7	MnPSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{GwOlYoRjJ7MEOxNFY3RC:jPh?=
HeLaS3	M{HpTGZ2dmO2aX;uJIF{e2G7	NFu2OGIyOCC3TR?=			MULJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTDt[YF{fXKnZDDpcY1m\GmjdHXsfUBjgSCrbX31co9jdG:2IHHuZYx6e2m\|	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N\|U9N2F-
HeLaS3	NFj0NZFHfW6ldHnvckBie3OjeR?=	M3Pkc\|ExKHWP	NX7QW2tZOTZiaILz		M{nLR2lvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjU{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm\|dH;u[UBJO0t7IHHj[ZR6dGG2aX;uJIF1KDFyIIXNJJRz\WG2ZXSg[o9zKDF4IHjyd{Bnd2yub4fl[EBjgSClb33wc5Vv\CC5YYPoc5V1KGGwZDDt[YF{fXKnZDD1dEB1dyB{NDDodpMh[nliaX3teY5w[myxdDDhcoFtgXOrcx?=	MlKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N\|UoRjNyMkW4OVM2RC:jPh?=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	EGFR / STAT3 ; Cyclin D1 / CDK6 / CDK4 / p-RB / RB ; MMP-1 / MMP-3 / MMP-13 / TIMP-1 ; Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1) ; α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2	31289542 27431944 23802098
Immunofluorescence	α-C/EBPβ / α-HP1α ; α-HDAC1 ; α-tubulin / acetylated histone 3	21122806 29045501 27957719
Growth inhibition assay	Cell viability (A549 cells) ; Cell viability (MDA-MB-231 cells) ; Cell viability (PC3 cells)	27571418 27548148 31289542

In vivo

Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. ^[1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. ^[5]

Protocol (from reference)

Kinase Assay: ^[1]	In vitro HDAC activity: Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×10⁵ cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 10⁶ cpm) of [³H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [³H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [³H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.
Cell Research: ^[1]	Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 Concentrations: Dissolved in absolute ethanol, final concentrations ~10 μM Incubation Time: 96 hours Method: Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion. (Only for Reference)
Animal Research: ^[1]	Animal Models: Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU Dosages: ~5 mg/kg/day Administration: Injection s.c. (Only for Reference)

References

+ Expand

Solubility (25°C)

In vitro	DMSO	23 mg/mL (76.05 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+2% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	1.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	302.4
Formula	C₁₇H₂₂N₂O₃
CAS No.	58880-19-6
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C

Download Trichostatin A (TSA) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04606771	Recruiting	Drug: Osimertinib + Savolitinib\|Drug: Savolitinib + Placebo	Non-Small Cell Lung Cancer	AstraZeneca	September 28 2020	Phase 2
NCT03901053	Recruiting	Device: Reaktiv AFO\|Device: PhatBrace AFO	Foot Injuries and Disorders	Jason Wilken\|Minneapolis Veterans Affairs Medical Center\|Walter Reed National Military Medical Center\|Henry M. Jackson Foundation for the Advancement of Military Medicine\|Center for Veterans Research and Education\|University of Delaware\|Johns Hopkins Bloomberg School of Public Health\|University of Iowa	February 27 2020	Not Applicable
NCT03887650	Recruiting	Drug: Liposomal Bupivicaine 1.3%\|Drug: Bupivacaine 0.5%	Post-operative Pain\|Total Shoulder Arthroplasty\|Osteoarthritis of the Shoulder\|Pain Management	Hartford Hospital	January 14 2019	Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Frequently Asked Questions

Question 1:
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

Answer:
Our S1045 Trichostatin A (TSA) is R enantiomer.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):