Trichostatin A (TSA)

Catalog No.S1045

For research use only.

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

Trichostatin A (TSA) Chemical Structure

CAS No. 58880-19-6

Selleck's Trichostatin A (TSA) has been cited by 217 publications

Purity & Quality Control

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Biological Activity

Description Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
Targets
HDAC [1]
(Cell-free assay)
~1.8 nM
In vitro

Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HeLa NX\rOJYyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrjNlAhdk1? MlvDO|IhcA>? NID3O2dFVVOR M3jVN2lEPTBib3[gNlBvVQ>? MmC2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ|MU[1O|Q5RC:jPh?=
HeLa M{DYcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHROFAhdk1? NHfPSGM1QCCq M3PVRmROW09? M2Pw[2lEPTBib3[gOFBvVQ>? NV\pNJVHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa MkfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\TXlExOCCwTR?= MXOyOEBp MUTEUXNQ NVG4PYsxUUN3MDDv[kAyODCwTR?= NFXaSok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2OVc1QCd-MkOxOlU4PDh:L3G+
Caco-2  NF3hTFRHfW6ldHnvckBCe3OjeR?= MW[xJOK2VcLi NWD0[Jp7OjRiaB?= NXjSd|c5\GWlcnXhd4V{KFOHUmSgdJJwfGWrbjDlfJBz\XO|aX;u MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7NUC3NEc,OjNzOUWwO|A9N2F-
HTK NWS1OoE4TnWwY4Tpc44hSXO|YYm= M2P5bFExOC16MEDuUS=> Mnf3O|IhcA>? M4XyXIJtd2OtczDUS2Yu|rMkgKPJcoR2[2WmIGLPV{BidmRiSELPNuKhSWOldX31cIF1cW:w MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
HTK M2TZO2Z2dmO2aX;uJGF{e2G7 NIrZPG01ODBibl2= MnjXO|IhcA>? MXvpcohq[mm2czDUS2Yu|rMkgKPJcoR2[2WmIF35c4Zq[nKxYnzhd5QhTGmoZnXy[Y51cWG2aX;u MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
HEK293 NWDNTI9YTnWwY4Tpc44hSXO|YYm= MlrYNUDDvU4EoB?= MXGxPEBpyqB? MX3pcohq[mm2czD0bIUhc2GuaYLpck04NW2nZHnheIVlKHKnY4L1bZRu\W62IH;mJJN6dnCqaXzpck0yKGGpZ4Ll[4F1\XNiaX70c{Bi\2e{ZYPvcYV{ NHWyV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4OFg1QCd-MkOyPFQ5PDh:L3G+
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K562 NVHtdVQ1SXCxcITvd4l{KEG|c3H5 NEfrW|IyKM7:TR?= NFHlTlIzPCCq MWLpcoR2[2W|wrDj[YxtKGSnYYTo MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRyMEWxPUc,OjN2MEC1NVk9N2F-
Kazumi NITQe29CeG:ydH;zbZMhSXO|YYm= NYjUNFBxOSEQvF2= Mn7FNlQhcA>? MX\pcoR2[2W|wrDj[YxtKGSnYYTo NVHYPW9nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
KG1 M1PIbWFxd3C2b4Ppd{BCe3OjeR?= M2HwV|Eh|ryP M{fMRlI1KGh? MXPpcoR2[2W|wrDj[YxtKGSnYYTo NYHBdWJsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
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HEL MoH4SpVv[3Srb36gRZN{[Xl? MXGwMlUh|ryP NHL1clczPCCq NY\0dldQ\XSqYX7vcC=> M3LxeIRm[3KnYYPld{B1cGViZX76fY1mKGGldHn2bZR6KG:oIFjERWN{KGOxLYTy[YF1dWWwdDD3bZRpKGO3cnP1cYlv MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR|MEm1O{c,OjN2M{C5OVc9N2F-
K562 MWfGeY5kfGmxbjDBd5NigQ>? MoDWNE42KM7:TR?= NWXzc2xMOjRiaB?= MWDleIhidm:u MYDk[YNz\WG|ZYOgeIhmKGWweont[UBi[3Srdnn0fUBw\iCKRFHDd{Bkdy22cnXheI1mdnRid3n0bEBkfXKldX3pci=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR|MEm1O{c,OjN2M{C5OVc9N2F-
LM8 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jBNlAvOyEQvF2= NWKxV4dTOTJvOU[gbC=> NHfoPGNFVVOR MXLpcohq[mm2czD0bIUh[2WubDDndo94fGhiY3:teJJm[XSvZX70JJdqfGhibXX0[o9zdWmw NWjNVm5IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVE5OTdpPkKzOFUyQDF5PD;hQi=>
MG-63  MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXH4RZdZOC5|IN88US=> NFfsXGwyOi17NjDo NInQb5ZFVVOR MknrbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHPvMZRz\WG2bXXueEB4cXSqIH3leIZwem2rbh?= MkTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUG4NVcoRjJ|NEWxPFE4RC:jPh?=
H1299 MYPGeY5kfGmxbjDBd5NigQ>? NUjkdXpIOC53IN88US=> MUK0PEBp M1HNWmROW09? MoS4bY5pcWKrdIOgeIhmKGmwdnHzbZZmdmW|c9MgJINwdWKrbnWge4l1cCC|aXzpZolvcW5? M4jwclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NE[xPVc2Lz5{M{S2NVk4PTxxYU6=
H1299 MlXNSpVv[3Srb36gRZN{[Xl? MWmwMlUh|ryP NGrqR2g1QCCq M4PKPWROW09? MmLHbY5pcWKrdIOgeIhmKG2rZ4LheI9zgSCyb4TlcpRq[WxiY3;tZolv\SC5aYToJJNqdGmkaX7pci=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR4MUm3OUc,OjN2NkG5O|U9N2F-
H1299  NXm4[HhnTnWwY4Tpc44hSXO|YYm= MXSwMlM{6oDVMdMgxtVO MXWyOE81QCCq MVjEUXNQ M{PDOolv[3KnYYPld{BGNWOjZHjldolvKHC{b4TlbY4hdGW4ZXzzJIRwe2ViZHXw[Y5l\W62bIm= MnX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NkG5O|UoRjJ|NE[xPVc2RC:jPh?=
MCF7 NF33d2FHfW6ldHnvckBCe3OjeR?= NVjLe|NTOC5yNT2yJO69VQ>? NHL0c4ozPCCq MomwdoVlfWOnczD0bIUhcW6mdXP0bY9vKG:oIIC1N{1l\XCnbnTlcpQh\2WwZYOgZpkhVnW2bHnuMVM> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR5MEW0NEc,OjN2N{C1OFA9N2F-
ARN8  MmrkSpVv[3Srb36gRZN{[Xl? MW[wMlA2NTJizszN NYTZbFUxOjRiaB?= NWj5XppTemWmdXPld{B1cGViaX7keYN1cW:wIH;mJJA2Oy2mZYDlcoRmdnRiZ3Xu[ZMh[nliToX0cIlvNTN? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR5MEW0NEc,OjN2N{C1OFA9N2F-
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A2780 M1O1[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPxNE4xPS1{IN88US=> NVHmUHVjOTBxMkSvOFghcA>? M2TpUmROW09? M1[4eY1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{M{iwNUc,OjR{MkO4NFE9N2F-
SKOV3 NX:0eJd7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfVNE4xPS1{IN88US=> M1\BfFExNzJ2L{S4JIg> NGHzeotFVVOR M13hfY1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ M1fRcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkKzPFAyLz5{NEKyN|gxOTxxYU6=
Huh7  MWjGeY5kfGmxbjDBd5NigQ>? NHfHbFAxNjFxMD61M|EvOCEQvF2= M4fWZVI1cA>? MU\zeZBxemW|c3XzJHlVUESFMjDn[Y5mKGW6cILld5Nqd25? NWnTNFU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3PzJpPkK0NlY6Pjd{PD;hQi=>
hMSCs M2jk[mZ2dmO2aX;uJGF{e2G7 MlThOk4zPSCwTR?= NHzZfo4zPCCq NUX0SVFkTE2VTx?= M36z[JN1[WKrbHn6[ZMhUGm|dH;u[UBC[2W2eXzheIlwdiCjbnSgeIhmKEW6cILld5Nqd25ib3[gVIx2emmyb4TlcpQhT2WwZYO= NFPOe4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOxNlM2Pid-MkSzNVI{PTZ:L3G+
HT29  NHzFVINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXexPFAhdk1? NGLLNVM4OiCq M3jyTWlEPTBib3[gNVgxKG6P MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4OEK2OUc,OjR|NkiyOlU9N2F-
RPE MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV:wMlIwOC52L{CuPE8yKM7:TR?= NFfDTpEzPC92OD:3NkBp NXPmcGVVTE2VTx?= NGXjZnZqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBjgSClZXzsJIN6[2ynIHHydoV{fA>? NYm1UYRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVY3ODJpPkK0OFU3PjB{PD;hQi=>
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U87 GBM M1joRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX2xNFAwOzByL{WwNEBvVQ>? NHm4b2g4OiCq MXuxNFAmKGW2aHHuc4w> NY\RZ5lMemWmdXPld{Bu\WGwIHPlcIwhdnWvYnXyJIJ6KDNzLDC1OEwh[W6mIEW4xsAm M4\EOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NE[0PFQyLz5{NES2OFg1OTxxYU6=
ACP03 M4nnNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3[4[|I2OC9|NUCvOVAxdk1? M4XiTFI1cA>? MYnk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlib3[gZZBxem:6aX3heIVtgSB5MNMgKUBifCB{NUFCpI5O NHXqcYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
ACP02 NFnQU|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLQd|czPTBxM{WwM|UxOG6P MWiyOIg> NFnXOXNl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P M{TSVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nk[4OVQ4Lz5{NE[2PFU1PzxxYU6=
HeLa M4XWPGZ2dmO2aX;uJGF{e2G7 NYjnUYJJOW2P MYewMlVp NVTJ[m1z[WKxbHnzbIV{KHSqZTD0c5RidCCKRFHDJIFkfGm4aYT5 NYDXcFdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NFc1PzRpPkK0O|A4PDd2PD;hQi=>
HMEC-1  Mlq4SpVv[3Srb36gRZN{[Xl? NH60emU{ODCwTR?= MWeyOIg> NYLJenQycW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzME[zNUc,OjR5MUC2N|E9N2F-
HeLa  MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofONu69VQ>? NV\PRo5rPDiq NWP3XVND\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIFjd3W2IEK1KS=> MnHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NE[xN|UoRjJ2OES2NVM2RC:jPh?=
LNCaP NGX4SVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrkNVAxNTFyMEDuUS=> MlPLNlRp NUjBUYExUUN3MDDv[kA{ODCwTR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh3NE[1PEc,OjR6NUS2OVg9N2F-
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A549 NX3FcnhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPTSoUxNjFvMd88US=> NV;5PGJHPDiq M4\CfGROW09? NHuxTlRKSzVyIH;mJFAvOjkQvF2= NGT0RnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
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SKOV-3 Mor1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDyc5NzOS1zMN88US=> NFTqUW0zPGh? NHu5cJhFVVOR MY\JR|UxKG:oIEWuOu69VQ>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
MCF7 M1rEcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4WxUVAuPjBybl2= Mn32NlRp MlTiSG1UVw>? NY\tTJFyUUN3MDDv[kA4PW6P NWX5SndHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPVI4OjFpPkK1NVkzPzJzPD;hQi=>
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A431 MW\GeY5kfGmxbjDBd5NigQ>? NEXuTpM2OG6P NHHXdXMzNzZxMUKvNlRp M3;UemROW09? NVLHZ4R3[WO2aY\heIV{KHB{MTDhcoQhcW6qaXLpeJMhSVSIMzDlfJBz\XO|aX;u M{f1OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{exNFY6Lz5{NUO3NVA3QTxxYU6=
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PC3  NUXKXGNVTnWwY4Tpc44hSXO|YYm= NV3XZmxUOC5zzszN Ml;COFhp M1vQSGROW09? NVqxcY53cW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?= MnrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
SW480  NF7UbZVHfW6ldHnvckBCe3OjeR?= NYPzPIRoOC5zzszN MXy0PIg> NGS4fmdFVVOR M37icIlv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ? NVfjUmwxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QTdpPkK1OFM1QTl5PD;hQi=>
PC3  NVT5T4RGTnWwY4Tpc44hSXO|YYm= NHP5[pUxNjIQvF2= MmLjOFhp NG\PXZBFVVOR M1LwOIF1fGWwdXH0[ZMhcW64YYPpc44h[W6mIH3p[5JifGmxbh?= M4\RWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
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PC12 M4i1b2Z2dmO2aX;uJIF{e2G7 NIDCcGQyOCC3TR?= MXu0PEBpenN? M{XwXmlvcGmkaYTpc44hd2ZiSFTBR|YhcW5icnH0JHBEOTJiY3XscJMh[XO|ZYPz[YQh[XNiYXPleJlt[XSrb36gc4YhcGm|dH;u[UBJOyCjdDCxNEB2VSCjZoTldkA1QCCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[{BidmGueYPpdy=> NGTkO2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
PC12 MXjGeY5kfGmxbjDhd5NigQ>? NVX1fHhZOTBidV2= NXzFc|BKPDhiaILz MlLmTY5pcWKrdHnvckBw\iCKRFHDOkBqdiC{YYSgVGMyOiClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxxcGFvdIXieYxqdiCjY3X0fYxifGmxbjDheEAyOCC3TTDh[pRmeiB2ODDodpMh[nliV3XzeIVzdiCkbH;0eIlv\yCjbnHsfZNqew>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
PC12 Ml72SpVv[3Srb36gZZN{[Xl? NWXnW5lFOTBidV2= NXqwWoNKPSCmYYnz M2jpTGlv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO= M4rYPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
SH-SY5Y NVzMSW1xTnWwY4Tpc44h[XO|YYm= NUCyfmN[OTBidV2= MV[1JIRigXN? MkPBTY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{ NHPScnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
A549 NEe3Z4dCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MnjtNE41KHWP M1nleVI1KGi{cx?= NF7CR49CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhe2i{dX7r[Y4h[W6mIIPoZZJxKHOqYYDlJIF1KDBwNDD1UUBi\nSncjCyOEBpenNiYomgdIhie2ViY3;ueJJie3RibXnjdo9{[2:yeR?= NHHjZWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4OFEyOid-MkW4PFQyOTJ:L3G+
HeLa Ml;XSpVv[3Srb36gZZN{[Xl? NUTGWmVEUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJJR2[nWuaX6gZYNmfHmuYYTpc44hcW6ldXLheIVlKG[xcjDveoVzdmmpaISgZpkhcW2vdX7v[ox2d3Knc3PlcoNmKG2rY4Lvd4NweGmlIHHuZYx6e2m| M3LhVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NkGxPVE6Lz5{Nk[xNVkyQTxxYU6=
C127-LT MlrESpVv[3Srb36gZZN{[Xl? Ml7MNlAxKG6P MlPaNlQhcHK| Mlf3TY5pcWKrdHnvckBw\iCKRFHDJIlvKG2xdYPlJGMyOjdvTGSgZ4VtdHNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iCHR1\QJIV5eHKnc4Ppc44h[XRiMkCwJI5OKGGodHXyJFI1KGi{czDifUBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yeR?= NYW1UHM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa nuclear NF7Qd4lHfW6ldHnvckBie3OjeR?= MUCxNEB1dyB{NTD1US=> NHjtdos{OCCvaX7z MojiR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHGgcpVkdGWjcjDj[YxteyCjdDCxNEB1dyB{NTD1UUB2e2mwZzDCc4Mu[WOndInsMYx6e2mwZT3BUWMh[XNic4Xid5Rz[XSnIHHmeIVzKDNyIH3pcpMh[nliTHnu[Zdm[X[ncj3CeZJsKHCub4SgZY5idHm|aYO= NF\aT2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa MYLGeY5kfGmxbjDhd5NigQ>? NVTpbpViOiC3TR?= M1PMblI1KGi{cx?= MWfJcohq[mm2aX;uJI9nKEiGQVOxJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiaInw[ZJi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KDJidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
HN13 M2nPT2Z2dmO2aX;uJIF{e2G7 NFyyclAzPCCqcoO= NYLyfmRpUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEiQMUOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGirc4TvcoUhUDNiTInzPUBi[2W2eXzheIlwdiCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBqdW23bn;mcJVwemW|Y3XuZ4Uh[XO|YYm= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MUC2Okc,OjlyM{GwOlY9N2F-
HeLaS3 M{TXbGZ2dmO2aX;uJIF{e2G7 NUTPdoVWOTBidV2= MkKxTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhmVGGVMzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBpcXO2b37lJGg{UzliYXPleJlt[XSrb36gZZQhOTBidV2gcYVie3W{ZXSgbY1u\WSrYYTlcJkh[nliaX3teY5w[myxdDDhcoFtgXOrcx?= NY\aTmR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyOVg2OzVpPkOwNlU5PTN3PD;hQi=>
HeLaS3 NVr2bZhETnWwY4Tpc44h[XO|YYm= MVexNEB2VQ>? NYnEbFY6OTZiaILz NITTfGpKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYWOzJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGirc4TvcoUhUDONOTDhZ4V1gWyjdHnvckBifCBzMDD1UUB1emWjdHXkJIZweiBzNjDodpMh\m:ubH;3[YQh[nliY3;tdI92dmRid3HzbI92fCCjbnSgcYVie3W{ZXSgeZAhfG9iMkSgbJJ{KGK7IHntcZVvd2Kub4SgZY5idHm|aYO= NH[3bJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK1PFU{PSd-M{CyOVg2OzV:L3G+
Assay
Methods Test Index PMID
Western blot EGFR / STAT3 ; Cyclin D1 / CDK6 / CDK4 / p-RB / RB ; MMP-1 / MMP-3 / MMP-13 / TIMP-1 ; Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1) ; α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2 31289542 27431944 23802098
Immunofluorescence α-C/EBPβ / α-HP1α ; α-HDAC1 ; α-tubulin / acetylated histone 3 21122806 29045501 27957719
Growth inhibition assay Cell viability (A549 cells) ; Cell viability (MDA-MB-231 cells) ; Cell viability (PC3 cells) 27571418 27548148 31289542
In vivo Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro HDAC activity:

    Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

Cell Research:

[1]

  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
  • Concentrations: Dissolved in absolute ethanol, final concentrations ~10 μM
  • Incubation Time: 96 hours
  • Method:

    Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

Animal Research:

[1]

  • Animal Models: Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
  • Dosages: ~5 mg/kg/day
  • Administration: Injection s.c.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

1.5mg/mL

Chemical Information

Molecular Weight 302.4
Formula

C17H22N2O3

CAS No. 58880-19-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT03901053 Recruiting Device: Reaktiv AFO|Device: PhatBrace AFO Foot Injuries and Disorders Jason Wilken|Minneapolis Veterans Affairs Medical Center|Walter Reed National Military Medical Center|Henry M. Jackson Foundation for the Advancement of Military Medicine|Center for Veterans Research and Education|University of Delaware|Johns Hopkins Bloomberg School of Public Health|University of Iowa February 27 2020 Not Applicable
NCT03887650 Recruiting Drug: Liposomal Bupivicaine 1.3%|Drug: Bupivacaine 0.5% Post-operative Pain|Total Shoulder Arthroplasty|Osteoarthritis of the Shoulder|Pain Management Hartford Hospital January 14 2019 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

Answer:
Our S1045 Trichostatin A (TSA) is R enantiomer.

Tags: buy Trichostatin A (TSA) | Trichostatin A (TSA) supplier | purchase Trichostatin A (TSA) | Trichostatin A (TSA) cost | Trichostatin A (TSA) manufacturer | order Trichostatin A (TSA) | Trichostatin A (TSA) distributor