Trichostatin A (TSA)

For research use only.

Catalog No.S1045

194 publications

Trichostatin A (TSA) Chemical Structure

CAS No. 58880-19-6

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

Selleck's Trichostatin A (TSA) has been cited by 194 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
Targets
HDAC [1]
(Cell-free assay)
~1.8 nM
In vitro

Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NEPkNFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3YNFNKOTBxM{CvOVAhdk1? NHzMVJk4OiCq NYDS[INPTE2VTx?= NEXrbJBqdmO{ZXHz[ZMhfGinIH71cYJmeiCxZjDNUXAhMM7WzrjtLUBtd3O|IHPlcIx{KGSxc3Wg[IVx\W6mZX70cJk> NUO4fG5kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml36NlAhdk1? NHnqN|I4OiCq Mkj6SG1UVw>? NX7hV|JYUUN3MDDv[kAzOG6P NEXhU5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2OVc1QCd-MkOxOlU4PDh:L3G+
HeLa MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3j2elQxKG6P MVy0PEBp MlLzSG1UVw>? NVLGT3FZUUN3MDDv[kA1OG6P M{jS[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MU[1O|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa Mk[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HxUlExOCCwTR?= NUjHSmNWOjRiaB?= M1LmXGROW09? NHHBWHJKSzVyIH;mJFExOG6P NULWRpVXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
Caco-2  NGHGcmhHfW6ldHnvckBCe3OjeR?= NHzRPW4yKML3TdMg NIHxWmwzPCCq NVrFU|l2\GWlcnXhd4V{KFOHUmSgdJJwfGWrbjDlfJBz\XO|aX;u MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7NUC3NEc,OjNzOUWwO|A9N2F-
HTK NHfTcW1HfW6ldHnvckBCe3OjeR?= MnvQNVAxNThyMH7N NYLVNWx5PzJiaB?= NIXtPYJjdG:la4OgWGdHNc7{4pETTY5lfWOnZDDSU3Mh[W6mIFiyU|LDqEGlY4XteYxifGmxbh?= MnfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OESwNFIoRjJ|Mki0NFAzRC:jPh?=
HTK NF\5b2ZHfW6ldHnvckBCe3OjeR?= MVu0NFAhdk1? MWW3NkBp M1G3ZolvcGmkaYTzJHRITi4QsvMAl2lv\HWlZXSgUZlw\mmkcn;icIF{fCCGaX\m[ZJmdnSrYYTpc44> MnTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OESwNFIoRjJ|Mki0NFAzRC:jPh?=
HEK293 NIjjdHpHfW6ldHnvckBCe3OjeR?= M1nOW|EhyrWPwrC= M1rLWlE5KGkEoB?= NX\0V2h7cW6qaXLpeJMhfGinIHvhcIlzcW5vNz3t[YRq[XSnZDDy[YNzfWm2bXXueEBw\iC|eX7wbIltcW5vMTDh[4dz\WejdHXzJIlvfG9iYXfndoV{d22ncx?= Mn3RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OES4OFgoRjJ|Mki0PFQ5RC:jPh?=
SH-SY5Y M4PVcWZ2dmO2aX;uJGF{e2G7 M4rocVI2OCCwTR?= M{TTTVE3KGh? M2rIO4Np[W6pZYOg[ZhxemW|c3nvckBx[XS2ZYLuJI9nKGenbnXzJIlvfm:udnXkJIlvKGOqb3zld5Rmem:uIIP5cpRp\XOrczygeZB1[WunIHHu[EBm\m[udYi= Mn;zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|Mk[0NlIoRjJ|M{K2OFIzRC:jPh?=
THP1 M1vlcGFxd3C2b4Ppd{BCe3OjeR?= M2\oXVEh|ryP NH3jNFEzPCCq M1z4Tolv\HWlZYRCpINmdGxiZHXheIg> NXvscoliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
K562 NF3DZYNCeG:ydH;zbZMhSXO|YYm= NYO4W4hoOSEQvF2= NGTKSZczPCCq NEPPN2NqdmS3Y3XzxsBk\WyuIHTlZZRp NUDvN|NKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
Kazumi M1XVNGFxd3C2b4Ppd{BCe3OjeR?= MmLCNUDPxE1? NIfpc4gzPCCq NUfXVWtkcW6mdXPld:Kh[2WubDDk[YF1cA>? NUf1XmQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
KG1 M1j4cWFxd3C2b4Ppd{BCe3OjeR?= M3Pm[FEh|ryP MUmyOEBp MVrpcoR2[2W|wrDj[YxtKGSnYYTo NXHyWYJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
HL60 NXTIU5BVSXCxcITvd4l{KEG|c3H5 MWKxJO69VQ>? NUC1fINOOjRiaB?= MonWbY5lfWOnc9MgZ4VtdCCmZXH0bC=> NW\BVYZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
HEL MlnDSpVv[3Srb36gRZN{[Xl? NGC1NW4xNjVizszN NWLCeFZLOjRiaB?= M3LrSYV1cGGwb3y= MV;k[YNz\WG|ZYOgeIhmKGWweont[UBi[3Srdnn0fUBw\iCKRFHDd{Bkdy22cnXheI1mdnRid3n0bEBkfXKldX3pci=> M1u1OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEOwPVU4Lz5{M{SzNFk2PzxxYU6=
K562 MoTDSpVv[3Srb36gRZN{[Xl? M4W0SVAvPSEQvF2= NWHuS5JKOjRiaB?= M3W2Z4V1cGGwb3y= M2\ybYRm[3KnYYPld{B1cGViZX76fY1mKGGldHn2bZR6KG:oIFjERWN{KGOxLYTy[YF1dWWwdDD3bZRpKGO3cnP1cYlv NVTXfIY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0N|A6PTdpPkKzOFMxQTV5PD;hQi=>
LM8 MkC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HhWVAvOyEQvF2= MkWyNVIuQTZiaB?= M1jRcmROW09? M{G5XIlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDjc{11emWjdH3lcpQhf2m2aDDt[ZRnd3KvaX6= NHrLdWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1NVgyPyd-MkO0OVE5OTd:L3G+
MG-63  MlrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXOwMlMh|ryP M{HRfVEzNTl4IHi= NFHuVnBFVVOR NIG3XYNqdmirYnn0d{B1cGViY3XscEBoem:5dHigZ48ufHKnYYTt[Y51KHerdHigcYV1\m:{bXnu NHzvPYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1NVgyPyd-MkO0OVE5OTd:L3G+
H1299 NVj4XFV4TnWwY4Tpc44hSXO|YYm= M4LIXFAvPSEQvF2= MlzwOFghcA>? M2PYZ2ROW09? NIi0XY5qdmirYnn0d{B1cGViaX72ZZNqfmWwZYPzxsAh[2:vYnnu[UB4cXSqIIPpcIljcW6rbh?= MmWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NkG5O|UoRjJ|NE[xPVc2RC:jPh?=
H1299 MYLGeY5kfGmxbjDBd5NigQ>? NIS1cGkxNjVizszN MnvDOFghcA>? MnXHSG1UVw>? MX7pcohq[mm2czD0bIUhdWmpcnH0c5J6KHCxdHXueIlidCClb33ibY5mKHerdHigd4ltcWKrbnnu MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR4MUm3OUc,OjN2NkG5O|U9N2F-
H1299  MWHGeY5kfGmxbjDBd5NigQ>? NHTlcGExNjN|4pETNeKhyrWP NFXFPXEzPC92ODDo M1HaOGROW09? MX3pcoNz\WG|ZYOgSU1k[WSqZYLpckBxem:2ZXnuJIxmfmWuczDkc5NmKGSncHXu[IVvfGy7 NH:1fZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S2NVk4PSd-MkO0OlE6PzV:L3G+
MCF7 Moj4SpVv[3Srb36gRZN{[Xl? MVWwMlA2NTJizszN NE[5SXQzPCCq M1jES5Jm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDwOVMu\GWyZX7k[Y51KGenbnXzJIJ6KE63dHzpck0{ Mn[1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{C1OFAoRjJ|NEewOVQxRC:jPh?=
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HASMCs MWDGeY5kfGmxbjDBd5NigQ>? MVqwMVUxOCCwTR?= MX22JIQ> M2LEdoRwf25vcnXneYxifGW|IITo[UBmgHC{ZYPzbY9vKG:oIFjBSGMh[W6mIHPlcIwhfmmjYnnsbZR6 MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVzOES2O{c,OjN3MUi0Olc9N2F-
MFE296 MULGeY5kfGmxbjDBd5NigQ>? NFrhUmU2ODBibl2= M2[3fFUh\A>? NXnOO|ZyTE2VTx?= Ml3ZbY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MEe2PUc,OjN3M{C3Olk9N2F-
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SRA01/04  NVzWUY5QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXVeIdOOC5{IN88US=> MkP2OFghcA>? M3u0VWROW09? MlzXd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM> NUHnVHI4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
A2780 Ml;uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1n5OFAvODVvMjFOwG0> MYSxNE8zPC92ODDo NUnUOJZ2TE2VTx?= M3XCWI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NG[wU|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN|gxOSd-MkSyNlM5ODF:L3G+
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hMSCs NGTRTINHfW6ldHnvckBCe3OjeR?= NX63NYF3Pi5{NTDuUS=> MYqyOEBp M{f3d2ROW09? NIPDfJl{fGGkaXzpfoV{KEirc4TvcoUhSWOndInsZZRqd25iYX7kJJRp\SCHeIDy[ZN{cW:wIH;mJHBtfXKrcH;0[Y51KEenbnXz NWHC[lQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVI{PTZpPkK0N|EzOzV4PD;hQi=>
HT29  MkDRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUKxPFAhdk1? NF7X[FE4OiCq NYDleXVVUUN3MDDv[kAyQDBibl2= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4OEK2OUc,OjR|NkiyOlU9N2F-
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U87 GBM MkC2SpVv[3Srb36gRZN{[Xl? MY[xNFAwPTByIF7t MkT3OFghcA>? NE\1UHgyODBnIHX0bIFvd2x? M2TNN2lv\HWlZYOgV4Vv\XOlZX7j[U1NcWunIFHseIVz[XSrb37zJIlvKE63Y3zlZZIhVW:{cHjvcI9ogQ>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
U87 GBM NXTmO5pHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\6V5UyODBxM{CwM|UxOCCwTR?= NXzWU4lJPzJiaB?= MYmxNFAmKGW2aHHuc4w> M12ydpJm\HWlZYOgcYVidiClZXzsJI52dWKncjDifUA{OSxiNUSsJIFv\CB3ONMgKS=> NXvEdVJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlQ5PDFpPkK0OFY1QDRzPD;hQi=>
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HeLa NHXFdlZHfW6ldHnvckBCe3OjeR?= M1r6OlFuVQ>? MnG3NE42cA>? M1vh[4Fjd2yrc3jld{B1cGVidH;0ZYwhUESDQzDhZ5Rqfmm2eR?= NHXOVWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEewO|Q4PCd-MkS3NFc1PzR:L3G+
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MCF7 NE\lc2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmP3NE03ODCwTR?= NXnGdJczOjSq NULXcGtUTE2VTx?= NGr3VVJKSzVyIH;mJFc2dk1? M4jCPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUmyO|IyLz5{NUG5NlczOTxxYU6=
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A431 NWrmdY5rTnWwY4Tpc44hSXO|YYm= Mkj6OVBvVQ>? M332cVIwPi9zMj:yOIg> MoTNSG1UVw>? MYDhZ5RqfmG2ZYOgdFIyKGGwZDDpcohq[mm2czDBWGY{KGW6cILld5Nqd25? M1n2bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{exNFY6Lz5{NUO3NVA3QTxxYU6=
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SW480  NUfpNmF5TnWwY4Tpc44hSXO|YYm= M17Nc|AvOc7:TR?= NYTKWmRvPDiq M3zFZWROW09? M4HieIlv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ? NYL5cGw6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QTdpPkK1OFM1QTl5PD;hQi=>
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melanoma B16/BL6 MlnZSpVv[3Srb36gZZN{[Xl? NEPWd4VKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcGm|dH;u[UBl\WGlZYT5cIF{\XNiKFjERWMyMSCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjBzOTFOwG0v NISycnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
melanoma B16/BL6 NV3meGRLTnWwY4Tpc44h[XO|YYm= NVrxeFl1UW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGirc4TvcoUh\GWjY3X0fYxie2ViKFjERWM1MSCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB{IN88UU4> M3L1RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MUC5OlI3Lz5zNUGwPVYzPjxxYU6=
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melanoma B16/BL6 NUPJVJlNTnWwY4Tpc44h[XO|YYm= Mk\FTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhQCCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB2IN88UU4> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
293T M4\FdWZ2dmO2aX;uJIF{e2G7 NEW2VnlKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViNDDwdoVx[XKnZDDmdo9uKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCyJO69VS5? NXO2dVhuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
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293T NHi0eWdHfW6ldHnvckBie3OjeR?= Mni0TY5pcWKrdHnvckBw\iCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNkDPxE1w MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzlyMEmxNUc,OTd7MEC5NVE9N2F-
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MCF7 NVPEbINbTnWwY4Tpc44h[XO|YYm= MWqwMlUhfU1? MWeyOEBpenN? NXG2OnBoUW6qaXLpeIlwdiCxZjDISGFEOSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJJVxNXKnZ4XsZZRqd25ib3[gZYNmfHmuYYTl[EBpcXO2b37lJGg{KHC{b4TlbY4hdGW4ZXygZZQhOC53IIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M1e3TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkiwN|Y{Lz5{MkK4NFM3OzxxYU6=
PC12 NVPyOFJMTnWwY4Tpc44h[XO|YYm= NVvYPVNOOTBidV2= MUW0PEBpenN? M33rOWlvcGmkaYTpc44hd2ZiSFTBR|YhcW5icnH0JHBEOTJiY3XscJMh[XO|ZYPz[YQh[XNiYXPleJlt[XSrb36gc4YhcGm|dH;u[UBJOyCjdDCxNEB2VSCjZoTldkA1QCCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[{BidmGueYPpdy=> NV;Ib2t{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
PC12 MmL0SpVv[3Srb36gZZN{[Xl? NIfYfVMyOCC3TR?= M4\OcVQ5KGi{cx?= M3\PWmlvcGmkaYTpc44hd2ZiSFTBR|YhcW5icnH0JHBEOTJiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36gZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IGfld5Rmem5iYnzveJRqdmdiYX7hcJl{cXN? NVLtUVl4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
PC12 NX7v[24yTnWwY4Tpc44h[XO|YYm= M1jHblExKHWP NGnLNnE2KGSjeYO= M3X0fWlv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO= M3nodlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
SH-SY5Y NEfJZ4ZHfW6ldHnvckBie3OjeR?= Mn;ONVAhfU1? Ml\IOUBl[Xm| MoXVTY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{ NGrodXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
A549 NYTsXIxVSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4rHXVAvPCC3TR?= NX;sWJh{OjRiaILz NGToUWpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhe2i{dX7r[Y4h[W6mIIPoZZJxKHOqYYDlJIF1KDBwNDD1UUBi\nSncjCyOEBpenNiYomgdIhie2ViY3;ueJJie3RibXnjdo9{[2:yeR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6NEGxNkc,OjV6OESxNVI9N2F-
HeLa NGS2ToRHfW6ldHnvckBie3OjeR?= MYXJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNidIXieYxqdiCjY3X0fYxifGmxbjDpcoN2[mG2ZXSg[o9zKG:4ZYLubYdpfCCkeTDpcY12dm:obIXvdoV{[2WwY3WgcYlkem:|Y3;wbYMh[W6jbInzbZM> NFTJTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[xNVkyQSd-Mk[2NVE6OTl:L3G+
C127-LT MVHGeY5kfGmxbjDhd5NigQ>? MUiyNFAhdk1? M2\wblI1KGi{cx?= NIP5VmZKdmirYnn0bY9vKG:oIFjERWMhcW5ibX;1d4UhSzF{Nz3MWEBk\WyuczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIFXHSnAh\XiycnXzd4lwdiCjdDCyNFAhdk1iYX\0[ZIhOjRiaILzJIJ6KG[udX;y[ZNk\W6lZTDtbYNzd3Olb4D5 NFH6XFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa nuclear M4rzXmZ2dmO2aX;uJIF{e2G7 NEHQOJcyOCC2bzCyOUB2VQ>? Mki0N|AhdWmwcx?= M1myd2NwdXCndHn0bZZmKGmwaHnibZRqd25ib3[gTGRCSyCrbjDoeY1idiCKZVzhJI52[2ynYYKgZ4VtdHNiYYSgNVAhfG9iMkWgeW0hfXOrbnegRo9kNWGlZYT5cE1tgXOrbnWtRW1EKGG|IIP1ZpN1emG2ZTDh[pRmeiB|MDDtbY5{KGK7IFzpcoV4\WG4ZYKtRpVzcyCybH;0JIFv[Wy7c3nz M1LQXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm2N|czLz5{Nkm5OlM4OjxxYU6=
HeLa NIq3fYpHfW6ldHnvckBie3OjeR?= NEDifGkzKHWP MlnnNlQhcHK| NUK3Wok2UW6qaXLpeIlwdiCxZjDISGFEOSCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIh6eGW{YXPleJlt[XSrb36gc4YhcGm|dH;u[UBJOyCjdDCyJJVOKGmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NFX6O5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
HN13 MWPGeY5kfGmxbjDhd5NigQ>? MnOzNlQhcHK| MoDrTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhPOTNiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIhqe3SxbnWgTFMhVHm|OTDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDpcY12dm:obIXvdoV{[2WwY3WgZZN{[Xl? NX7NUZlzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|ExPjZpPkK5NFMyODZ4PD;hQi=>
HeLaS3 NHjNVZZHfW6ldHnvckBie3OjeR?= MV:xNEB2VQ>? M{\x[WlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjU{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm|dH;u[UBJO0t7IHHj[ZR6dGG2aX;uJIF1KDFyIIXNJI1m[XO3cnXkJIludWWmaXH0[Yx6KGK7IHntcZVvd2Kub4SgZY5idHm|aYO= MlPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N|UoRjNyMkW4OVM2RC:jPh?=
HeLaS3 MmnHSpVv[3Srb36gZZN{[Xl? NUfnemxxOTBidV2= M1f6[FE3KGi{cx?= M{LLW2lvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjU{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm|dH;u[UBJO0t7IHHj[ZR6dGG2aX;uJIF1KDFyIIXNJJRz\WG2ZXSg[o9zKDF4IHjyd{Bnd2yub4fl[EBjgSClb33wc5Vv\CC5YYPoc5V1KGGwZDDt[YF{fXKnZDD1dEB1dyB{NDDodpMh[nliaX3teY5w[myxdDDhcoFtgXOrcx?= MnGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N|UoRjNyMkW4OVM2RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
EGFR / STAT3 ; 

PubMed: 31289542     


Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting.

Cyclin D1 / CDK6 / CDK4 / p-RB / RB; 

PubMed: 31289542     


Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. 

MMP-1 / MMP-3 / MMP-13 / TIMP-1; 

PubMed: 27431944     


Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h.

Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); 

PubMed: 27431944     


Chondrocytes were treated with increasing concentrations of TSA for 24 h.

α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; 

PubMed: 23802098     


Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).

31289542 27431944 23802098
Immunofluorescence
α-C/EBPβ / α-HP1α ; 

PubMed: 21122806     


3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. 

α-HDAC1 ; 

PubMed: 29045501     


MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels.

α-tubulin / acetylated histone 3; 

PubMed: 27957719     


Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.

21122806 29045501 27957719
Growth inhibition assay
Cell viability (A549 cells); 

PubMed: 27571418     


Cytotoxicity of histamine determined by MTT assay in A549 cells.

Cell viability (MDA-MB-231 cells); 

PubMed: 27548148     


The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8.

Cell viability (PC3 cells); 

PubMed: 31289542     


PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.

27571418 27548148 31289542
In vivo Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]

Protocol

Kinase Assay:

[1]

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In vitro HDAC activity:

Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.
Cell Research:

[1]

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  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
  • Concentrations: Dissolved in absolute ethanol, final concentrations ~10 μM
  • Incubation Time: 96 hours
  • Method:

    Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
  • Dosages: ~5 mg/kg/day
  • Administration: Injection s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (76.05 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 302.4
Formula

C17H22N2O3

CAS No. 58880-19-6
Storage powder
in solvent
Synonyms N/A
Smiles CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT03901053 Recruiting Device: Reaktiv AFO|Device: PhatBrace AFO Foot Injuries and Disorders Jason Wilken|Minneapolis Veterans Affairs Medical Center|Walter Reed National Military Medical Center|Henry M. Jackson Foundation for the Advancement of Military Medicine|Center for Veterans Research and Education|University of Delaware|Johns Hopkins Bloomberg School of Public Health|University of Iowa February 27 2020 Not Applicable
NCT03887650 Recruiting Drug: Liposomal Bupivicaine 1.3%|Drug: Bupivacaine 0.5% Post-operative Pain|Total Shoulder Arthroplasty|Osteoarthritis of the Shoulder|Pain Management Hartford Hospital January 14 2019 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

  • Answer:

    Our S1045 Trichostatin A (TSA) is R enantiomer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID