Trichostatin A (TSA)

For research use only.

Catalog No.S1045

185 publications

Trichostatin A (TSA) Chemical Structure

CAS No. 58880-19-6

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

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Selleck's Trichostatin A (TSA) has been cited by 185 publications

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Biological Activity

Description Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
Targets
HDAC [1]
(Cell-free assay)
~1.8 nM
In vitro

Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HeLa NXX3T|BHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHv0SIUzOCCwTR?= M4jFTVczKGh? M1GwU2ROW09? NXLvXFdZUUN3MDDv[kAzOG6P NUfvNFVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa NFTYXGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3riOVQxKG6P MUi0PEBp MVrEUXNQ NEPyWZhKSzVyIH;mJFQxdk1? Ml31QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ|MU[1O|Q5RC:jPh?=
HeLa MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPaNVAxKG6P NEO5VGgzPCCq M2ewPWROW09? MorqTWM2OCCxZjCxNFBvVQ>? M1PtO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MU[1O|Q5Lz5{M{G2OVc1QDxxYU6=
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HTK MoDvSpVv[3Srb36gRZN{[Xl? Mn[zNVAxNThyMH7N NWfVWHJyPzJiaB?= M3K5WYJtd2OtczDUS2Yu|rMkgKPJcoR2[2WmIGLPV{BidmRiSELPNuKhSWOldX31cIF1cW:w MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
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SRA01/04  MkTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzvO24xNjJizszN MUm0PEBp MljMSG1UVw>? MoKzd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM> NWHtbo5pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
A2780 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLXR|MxNjB3LUKg{txO MVyxNE8zPC92ODDo MV;EUXNQ MkHmcYVlcWG2ZYOg[5Jwf3SqIHHydoV{fCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NXjCUWIzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlM5ODFpPkK0NlI{QDBzPD;hQi=>
SKOV3 NHz2dJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DrfVAvODVvMjFOwG0> M1\vdFExNzJ2L{S4JIg> MnzlSG1UVw>? MVjt[YRq[XSnczDndo94fGhiYYLy[ZN1KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? NGfzdmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN|gxOSd-MkSyNlM5ODF:L3G+
Huh7  NXu5TlJETnWwY4Tpc44hSXO|YYm= Ml;4NE4yNzBwNT:xMlAh|ryP NEDqSWQzPGh? M3zldpN2eHC{ZYPz[ZMhYVSKRFOyJIdmdmViZYjwdoV{e2mxbh?= NVu4RoJjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3PzJpPkK0NlY6Pjd{PD;hQi=>
hMSCs Ml\wSpVv[3Srb36gRZN{[Xl? NHnkRog3NjJ3IH7N M3zOUFI1KGh? MmjZSG1UVw>? MYrzeIFjcWyrenXzJGhqe3SxbnWgRYNmfHmuYYTpc44h[W6mIITo[UBGgHC{ZYPzbY9vKG:oIGDseZJqeG:2ZX70JGdmdmW| MmjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUKzOVYoRjJ2M{GyN|U3RC:jPh?=
HT29  NFLJXZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\ENVgxKG6P MkjBO|IhcA>? NF7NeG1KSzVyIH;mJFE5OCCwTR?= MmCwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkiyOlUoRjJ2M{[4NlY2RC:jPh?=
RPE NEC1NllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjnNWk2OC5{L{CuOE8xNjhxMTFOwG0> MVWyOE81QC95MjDo NFrLfI5FVVOR MmS3bY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gZpkh[2WubDDjfYNt\SCjcoLld5Q> NYe2VotLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVY3ODJpPkK0OFU3PjB{PD;hQi=>
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U87 GBM MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zaSVExOC9|MECvOVAxKG6P NXTlWmxNPzJiaB?= NYfDOJpkOTByJTDleIhidm:u MY\y[YR2[2W|IH3lZY4h[2WubDDueY1j\XJiYomgN|EtKDV2LDDhcoQhPTkEoDW= NXXqd5k4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlQ5PDFpPkK0OFY1QDRzPD;hQi=>
ACP03 NFjUS|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTnNlUxNzN3MD:1NFBvVQ>? NVH4e245OjSq M1nwOYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1? NF;qdYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
ACP02 NEjGXZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXWyV5Z6OjVyL{O1NE82ODCwTR?= MY[yOIg> MYHk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlib3[gZZBxem:6aX3heIVtgSB5MNMgKUBifCB{NUFCpI5O NH\Z[4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
HeLa MmT1SpVv[3Srb36gRZN{[Xl? NEPXXJEydU1? NEHlO3kxNjWq NULtWHFu[WKxbHnzbIV{KHSqZTD0c5RidCCKRFHDJIFkfGm4aYT5 NVjCUmM6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NFc1PzRpPkK0O|A4PDd2PD;hQi=>
HMEC-1  M{f5SGZ2dmO2aX;uJGF{e2G7 M4TQ[VMxOG6P NGPsSoczPGh? MVfpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\sLiVlXHSnI{yqCvUl7B MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzME[zNUc,OjR5MUC2N|E9N2F-
HeLa  MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrNToIz|ryP NX;UflhFPDiq NH;X[ndl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZYJwfXRiMkWl MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2NkGzOUc,OjR6NE[xN|U9N2F-
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PC3 NXq0eG04T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjqNVAxNTFyMEDuUS=> NWTTUZN[OjSq NH\2ZohKSzVyIH;mJFMxOG6P NI\vXWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi1OFY2QCd-MkS4OVQ3PTh:L3G+
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A549 NVPyUoZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV[wMlAyNTBwOd88US=> MknCO|Jp MX;EUXNQ NIjWcHlKSzVyIH;mJFAvODcQvF2= NXrWS2w5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
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A549 NXjIW4g4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYGxMVEx|ryP NELyVFgzPGh? M2HtRWROW09? NGHFW4JKSzVyIH;mJFMvOs7:TR?= NH\pT5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
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MCF7 NXfubnF5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3SwUFAuPjBybl2= MoTkNlRp M{frbGROW09? NYnq[ZJPUUN3MDDv[kA4PW6P MlLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUK3NlEoRjJ3MUmyO|IyRC:jPh?=
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A431 MkS4SpVv[3Srb36gRZN{[Xl? MWC1NI5O MkPVNk83NzF{L{K0bC=> Ml\XSG1UVw>? NYnafWtP[WO2aY\heIV{KHB{MTDhcoQhcW6qaXLpeJMhSVSIMzDlfJBz\XO|aX;u NEXXUIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO3NVA3QSd-MkWzO|ExPjl:L3G+
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PC3  NVv0dFJGTnWwY4Tpc44hSXO|YYm= NUCzfZNLOC5zzszN MmqxOFhp MlnySG1UVw>? MXrpcoR2[2W|IHnuZ5Jm[XOnIH;mJGhFSUNzIHHu[EBJTEGFMjDvckBUdHWpIHflcoV{KHC{b33veIVz MmXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
SW480  MYXGeY5kfGmxbjDBd5NigQ>? NF6zcFgxNjIQvF2= NGPKdVM1QGh? Ml;USG1UVw>? M1TLcolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ? NEmweWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOFk6Pyd-MkW0N|Q6QTd:L3G+
PC3  MXPGeY5kfGmxbjDBd5NigQ>? MXSwMlHPxE1? NWDhboY5PDiq NXL5PVRxTE2VTx?= NX7WfoN6[XS2ZX71ZZRmeyCrbo\hd4lwdiCjbnSgcYloemG2aX;u M4LwcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
PC3  M4HJdGZ2dmO2aX;uJGF{e2G7 NG\ubmUxNjIQvF2= NYTmdIx[PDiq MXjEUXNQ NHW2dWFz\X[ncoPld{BGVVR? M{fXcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
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melanoma B16/BL6 MlfGSpVv[3Srb36gZZN{[Xl? M3PjPWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKCiKRFHDOEkheHKncHHy[YQh\nKxbTDtc5V{\SCvZXzhco9u[SCEMU[vRmw3KGOnbHzzMEBKSzVyIE2gNE4xOiEQvF2u NU\nNnRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxNFk3OjZpPkG1NVA6PjJ4PD;hQi=>
melanoma B16/BL6 M3vNPGZ2dmO2aX;uJIF{e2G7 NXfK[INmUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGirc4TvcoUh\GWjY3X0fYxie2ViNjDwdoVx[XKnZDDmdo9uKG2xdYPlJI1mdGGwb33hJGIyPi:ETE[gZ4VtdHNuIFnDOVAhRSByLkCyPEDPxE1w M4PNVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MUC5OlI3Lz5zNUGwPVYzPjxxYU6=
melanoma B16/BL6 MoDsSpVv[3Srb36gZZN{[Xl? NWH4[YxxUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGirc4TvcoUh\GWjY3X0fYxie2ViODDwdoVx[XKnZDDmdo9uKG2xdYPlJI1mdGGwb33hJGIyPi:ETE[gZ4VtdHNuIFnDOVAhRSByLkC0JO69VS5? M3f6[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MUC5OlI3Lz5zNUGwPVYzPjxxYU6=
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293T NYDMdog3TnWwY4Tpc44h[XO|YYm= M2rPOmlvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SB6IIDy[ZBiemWmIH\yc40hOjl|VDDj[YxteyxiSVO1NEA:KDBwMESg{txONg>? MkKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
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H661 MoLZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M176flQ5KGi{cx?= NFTXUJRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGg3PjFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHhVXCCjc4PhfUwhUUN3MDC9JFAvODh3IN88UU4> MknqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MkS3O|MoRjF5NkK0O|c{RC:jPh?=
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293T MYLGeY5kfGmxbjDhd5NigQ>? NEfCSIJKdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOxJIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy|LDDJR|UxKD1iMD6wNFE6KM7:TT6= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh6MUKzNkc,OTd6OEGyN|I9N2F-
293T MmTFSpVv[3Srb36gZZN{[Xl? NUf0WWlRUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFAzKM7:TT6= NVeweIJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PFEzOzJpPkG3PFgyOjN{PD;hQi=>
293T M3m5UGZ2dmO2aX;uJIF{e2G7 MmXYTY5pcWKrdHnvckBw\iCvb4Xz[UBJTEGFNjDlfJBz\XO|ZXSgbY4hOjl|VDDj[YxteyxiSVO1NEA:KDBwMECyPEDPxE1w Mnz6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN|IoRjF5OEixNlMzRC:jPh?=
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293T MmHKSpVv[3Srb36gZZN{[Xl? M1L1VWlvcGmkaYTpc44hd2ZiSFTBR|Eh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlE6KM7:TT6= M2ewclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUCwPVEyLz5zN{mwNFkyOTxxYU6=
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293T NVK3[3pbTnWwY4Tpc44h[XO|YYm= MlPiTY5pcWKrdHnvckBw\iCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNlgh|ryPLh?= M4LrSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUCwPVEyLz5zN{mwNFkyOTxxYU6=
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neonatal foreskin fibroblasts MoPLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoG4S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gcoVwdmG2YXyg[o9z\XOtaX6g[oljem:kbHHzeJMh[2WubIOsJGlEPTBiPTCwMlIh|ryPLh?= NGDUO|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O|U2PCd-MUiyOFc2PTR:L3G+
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MCF7 NFnV[2VHfW6ldHnvckBie3OjeR?= MYewMlUhfU1? M3\YcFI1KGi{cx?= MYTJcohq[mm2aX;uJI9nKEiGQVOxJIlvKGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNidYCtdoVofWyjdHnvckBw\iCjY3X0fYxifGWmIHjpd5RwdmViSEOgdJJwfGWrbjDs[ZZmdCCjdDCwMlUhfU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
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PC12 M3TXXWZ2dmO2aX;uJIF{e2G7 M13IRVExKHWP NHXLdGI1QCCqcoO= Mk\qTY5pcWKrdHnvckBw\iCKRFHDOkBqdiC{YYSgVGMyOiClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxxcGFvdIXieYxqdiCjY3X0fYxifGmxbjDheEAyOCC3TTDh[pRmeiB2ODDodpMh[nliV3XzeIVzdiCkbH;0eIlv\yCjbnHsfZNqew>? MmjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
PC12 NHfyTGxHfW6ldHnvckBie3OjeR?= MmnsNVAhfU1? NIPKTI42KGSjeYO= MmXsTY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIILheEBRSzF{IHPlcIx{KGG2IEGwJJVOKGGodHXyJFUh\GG7cx?= M4H0OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
SH-SY5Y NEfURYpHfW6ldHnvckBie3OjeR?= NGTVbmYyOCC3TR?= NV\MTpdkPSCmYYnz MWDJcoR2[3Srb36gc4YhdmW3cnn0[UBwfXSpcn;3eIghcW5iaIXtZY4hW0hvU2m1XUBk\WyuczDheEAyOCC3TTDh[pRmeiB3IHThfZM> NWThc45URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
A549 MnfYRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGS0XIExNjRidV2= MVyyOEBpenN? M1PCdWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{B{cHK3bnvlckBidmRic3jhdpAhe2ijcHWgZZQhOC52IIXNJIFnfGW{IEK0JIhzeyCkeTDwbIF{\SClb370doF{fCCvaXPyc5Nkd3C7 Mn;uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OESxNVIoRjJ3OEi0NVEzRC:jPh?=
HeLa NFnTNnVHfW6ldHnvckBie3OjeR?= M2HqSmlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczD0eYJ2dGmwIHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3Jib4\ldo5q\2i2IHL5JIludXWwb3\seY9z\XOlZX7j[UBucWO{b4Pjc5Bq[yCjbnHsfZNqew>? NXPv[GliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2NVE6OTlpPkK2OlEyQTF7PD;hQi=>
C127-LT NHXy[IpHfW6ldHnvckBie3OjeR?= M4j6WlIxOCCwTR?= MV6yOEBpenN? MW\Jcohq[mm2aX;uJI9nKEiGQVOgbY4hdW:3c3WgR|EzPy2OVDDj[YxteyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKEWJRmCg[ZhxemW|c3nvckBifCB{MECgcm0h[W[2ZYKgNlQhcHK|IHL5JIZtfW:{ZYPj[Y5k\SCvaXPyc5Nkd3C7 NYrTbFVGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa nuclear MXvGeY5kfGmxbjDhd5NigQ>? NGHqdogyOCC2bzCyOUB2VQ>? NFfPVo8{OCCvaX7z M3nNb2NwdXCndHn0bZZmKGmwaHnibZRqd25ib3[gTGRCSyCrbjDoeY1idiCKZVzhJI52[2ynYYKgZ4VtdHNiYYSgNVAhfG9iMkWgeW0hfXOrbnegRo9kNWGlZYT5cE1tgXOrbnWtRW1EKGG|IIP1ZpN1emG2ZTDh[pRmeiB|MDDtbY5{KGK7IFzpcoV4\WG4ZYKtRpVzcyCybH;0JIFv[Wy7c3nz Mki4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7OU[zO|IoRjJ4OUm2N|czRC:jPh?=
HeLa M4X1V2Z2dmO2aX;uJIF{e2G7 MlT1NkB2VQ>? M2PT[FI1KGi{cx?= MXLJcohq[mm2aX;uJI9nKEiGQVOxJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiaInw[ZJi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KDJidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NGrGb4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
HN13 M2DycGZ2dmO2aX;uJIF{e2G7 NVvUO5NOOjRiaILz M3W5XGlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJVjF|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBpcXO2b37lJGg{KEy7c{mgZYNmfHmuYYTpc44hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJIF{e2G7 M{X6N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEOxNFY3Lz5{OUCzNVA3PjxxYU6=
HeLaS3 MVzGeY5kfGmxbjDhd5NigQ>? M3vYelExKHWP MmXSTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhmVGGVMzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBpcXO2b37lJGg{UzliYXPleJlt[XSrb36gZZQhOTBidV2gcYVie3W{ZXSgbY1u\WSrYYTlcJkh[nliaX3teY5w[myxdDDhcoFtgXOrcx?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N|U9N2F-
HeLaS3 MnTjSpVv[3Srb36gZZN{[Xl? NHi3[HkyOCC3TR?= MX:xOkBpenN? MUnJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN|IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTD0doVifGWmIH\vdkAyPiCqcoOg[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G|aH;1eEBidmRibXXhd5Vz\WRidYCgeI8hOjRiaILzJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXN? M2nnOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkW4OVM2Lz5|MEK1PFU{PTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
EGFR / STAT3 ; 

PubMed: 31289542     


Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting.

Cyclin D1 / CDK6 / CDK4 / p-RB / RB; 

PubMed: 31289542     


Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. 

MMP-1 / MMP-3 / MMP-13 / TIMP-1; 

PubMed: 27431944     


Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h.

Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); 

PubMed: 27431944     


Chondrocytes were treated with increasing concentrations of TSA for 24 h.

α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; 

PubMed: 23802098     


Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).

31289542 27431944 23802098
Immunofluorescence
α-C/EBPβ / α-HP1α ; 

PubMed: 21122806     


3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. 

α-HDAC1 ; 

PubMed: 29045501     


MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels.

α-tubulin / acetylated histone 3; 

PubMed: 27957719     


Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.

21122806 29045501 27957719
Growth inhibition assay
Cell viability (A549 cells); 

PubMed: 27571418     


Cytotoxicity of histamine determined by MTT assay in A549 cells.

Cell viability (MDA-MB-231 cells); 

PubMed: 27548148     


The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8.

Cell viability (PC3 cells); 

PubMed: 31289542     


PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.

27571418 27548148 31289542
In vivo Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]

Protocol

Kinase Assay:

[1]

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In vitro HDAC activity:

Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.
Cell Research:

[1]

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  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
  • Concentrations: Dissolved in absolute ethanol, final concentrations ~10 μM
  • Incubation Time: 96 hours
  • Method:

    Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
  • Dosages: ~5 mg/kg/day
  • Administration: Injection s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (76.05 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
1.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 302.4
Formula

C17H22N2O3

CAS No. 58880-19-6
Storage powder
in solvent
Synonyms N/A
Smiles CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03887650 Unknown status Drug: Liposomal Bupivicaine 1.3%|Drug: Bupivacaine 0.5% Post-operative Pain|Total Shoulder Arthroplasty|Osteoarthritis of the Shoulder|Pain Management Hartford Hospital January 14 2019 Phase 4
NCT03838926 Unknown status Drug: Trichostatin A Relapsed or Refractory Hematologic Malignancies Vanda Pharmaceuticals September 27 2018 Phase 1
NCT02976272 Unknown status Other: bortezomib Multiple Myeloma|Bortezomib Regimen|Adult University Hospital Clermont-Ferrand|Laboratoires Takeda June 7 2016 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

  • Answer:

    Our S1045 Trichostatin A (TSA) is R enantiomer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID