Trichostatin A (TSA)

Catalog No.S1045

For research use only.

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

Trichostatin A (TSA) Chemical Structure

CAS No. 58880-19-6

Selleck's Trichostatin A (TSA) has been cited by 203 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Other HDAC Products

Biological Activity

Description Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
Targets
HDAC [1]
(Cell-free assay)
~1.8 nM
In vitro

Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NWDhWIRpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTRNVAwOzBxNUCgcm0> NEXWdGo4OiCq NGfPVXFFVVOR MXPpcoNz\WG|ZYOgeIhmKG63bXLldkBw\iCPTWCgLO6V|qivKTDsc5N{KGOnbHzzJIRwe2ViZHXw[Y5l\W62bIm= NWC4N4lERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXKyNEBvVQ>? NFPXUpQ4OiCq M3nkd2ROW09? NVXE[FZjUUN3MDDv[kAzOG6P M3PuelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MU[1O|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa NYPQS5dlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTtOYdFPDBibl2= NFSyTIU1QCCq Ml7zSG1UVw>? NYLLfolqUUN3MDDv[kA1OG6P M4\yXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MU[1O|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa M{[wdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX2xNFAhdk1? MYGyOEBp M4H3XmROW09? Mlj2TWM2OCCxZjCxNFBvVQ>? NYnOUYdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
Caco-2  MlPrSpVv[3Srb36gRZN{[Xl? NHjaVoYyKML3TdMg NFvUVY4zPCCq MX;k[YNz\WG|ZYOgV2VTXCCycn;0[YlvKGW6cILld5Nqd25? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7NUC3NEc,OjNzOUWwO|A9N2F-
HTK M4DYZ2Z2dmO2aX;uJGF{e2G7 MnLiNVAxNThyMH7N NG\ER484OiCq MV7icI9kc3NiVFfGMe6z6oDVSX7keYNm\CCUT2OgZY5lKEh{T{NCpGFk[3WvdXzheIlwdg>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
HTK MlfMSpVv[3Srb36gRZN{[Xl? NVmyUHB[PDByIH7N NXTqR5FOPzJiaB?= NU\oUGpEcW6qaXLpeJMhXEeILd8y5qCUUW6mdXPl[EBOgW:oaXLyc4Jt[XO2IFTp[oZmemWwdHnheIlwdg>? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NECwNkc,OjN{OESwNFI9N2F-
HEK293 NX;VUmQ{TnWwY4Tpc44hSXO|YYm= MYSxJOK2VcLi MnOyNVghcMLi NV[0WIxmcW6qaXLpeJMhfGinIHvhcIlzcW5vNz3t[YRq[XSnZDDy[YNzfWm2bXXueEBw\iC|eX7wbIltcW5vMTDh[4dz\WejdHXzJIlvfG9iYXfndoV{d22ncx?= M1L1bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mki0PFQ5Lz5{M{K4OFg1QDxxYU6=
SH-SY5Y M3XXcGZ2dmO2aX;uJGF{e2G7 MXeyOVAhdk1? NXvFfFk2OTZiaB?= MVfjbIFv\2W|IHX4dJJme3Orb36gdIF1fGW{bjDv[kBo\W6nczDpcpZwdH[nZDDpckBkcG:uZYP0[ZJwdCC|eX70bIV{cXNuIIXweIFs\SCjbnSg[YZndHW6 MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{NkSyNkc,OjN|Mk[0NlI9N2F-
THP1 NHSzc4pCeG:ydH;zbZMhSXO|YYm= NUGwO5RQOSEQvF2= NWe1VXNZOjRiaB?= MWjpcoR2[2W|wrDj[YxtKGSnYYTo NXviTVh6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
K562 MoPzRZBweHSxc3nzJGF{e2G7 M4TQdFEh|ryP MXSyOEBp MlnNbY5lfWOnc9MgZ4VtdCCmZXH0bC=> NXLiSpJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
Kazumi Mnz1RZBweHSxc3nzJGF{e2G7 M3LET|Eh|ryP M2XjZ|I1KGh? M4nBPIlv\HWlZYRCpINmdGxiZHXheIg> M1;lfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NECwOVE6Lz5{M{SwNFUyQTxxYU6=
KG1 Mm\iRZBweHSxc3nzJGF{e2G7 MWixJO69VQ>? NFnvR|MzPCCq NUSwZmxJcW6mdXPld:Kh[2WubDDk[YF1cA>? Ml24QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEC1NVkoRjJ|NECwOVE6RC:jPh?=
HL60 MWfBdI9xfG:|aYOgRZN{[Xl? M1zQeVEh|ryP M3rDOlI1KGh? MlnsbY5lfWOnc9MgZ4VtdCCmZXH0bC=> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRyMEWxPUc,OjN2MEC1NVk9N2F-
HEL NFrycYhHfW6ldHnvckBCe3OjeR?= NI\2R|gxNjVizszN NYXMcpRkOjRiaB?= NEW0UGNmfGijbn;s NFnpTJRl\WO{ZXHz[ZMhfGinIHXufplu\SCjY4Tpeol1gSCxZjDISGFEeyClbz30doVifG2nboSge4l1cCCldYLjeY1qdg>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR|MEm1O{c,OjN2M{C5OVc9N2F-
K562 MlW2SpVv[3Srb36gRZN{[Xl? MkTONE42KM7:TR?= M4n5[FI1KGh? Mn;W[ZRp[W6xbB?= NV;mRYU2\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5? MnP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2M{C5OVcoRjJ|NEOwPVU4RC:jPh?=
LM8 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITGOVUxNjNizszN Mk[xNVIuQTZiaB?= M1fCXWROW09? NVi1dox5cW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJINwNXS{ZXH0cYVvfCC5aYToJI1mfG[xcn3pci=> M2\xR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEWxPFE4Lz5{M{S1NVgyPzxxYU6=
MG-63  NX;6ZpQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLpNE4{KM7:TR?= NHTJWJgyOi17NjDo NIjndWZFVVOR Mn7xbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHPvMZRz\WG2bXXueEB4cXSqIH3leIZwem2rbh?= M{jhPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEWxPFE4Lz5{M{S1NVgyPzxxYU6=
H1299 MXnGeY5kfGmxbjDBd5NigQ>? NGfaVW4xNjVizszN M{DyNlQ5KGh? MmXuSG1UVw>? NYr5VlJ4cW6qaXLpeJMhfGinIHnueoF{cX[nbnXzd:KhKGOxbXLpcoUhf2m2aDDzbYxq[mmwaX6= MkG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NkG5O|UoRjJ|NE[xPVc2RC:jPh?=
H1299 MmnrSpVv[3Srb36gRZN{[Xl? MXuwMlUh|ryP MX20PEBp NX3YOVJITE2VTx?= MkTBbY5pcWKrdIOgeIhmKG2rZ4LheI9zgSCyb4TlcpRq[WxiY3;tZolv\SC5aYToJJNqdGmkaX7pci=> M2TGOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NE[xPVc2Lz5{M{S2NVk4PTxxYU6=
H1299  M2PNeWZ2dmO2aX;uJGF{e2G7 NYfKXVBNOC5|M,MAl|HDqML3TR?= M3TrflI1NzR6IHi= NWDUbXB5TE2VTx?= MUfpcoNz\WG|ZYOgSU1k[WSqZYLpckBxem:2ZXnuJIxmfmWuczDkc5NmKGSncHXu[IVvfGy7 NGKwc|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S2NVk4PSd-MkO0OlE6PzV:L3G+
MCF7 M375XWZ2dmO2aX;uJGF{e2G7 MWqwMlA2NTJizszN NHrjU5IzPCCq M2W5NZJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDwOVMu\GWyZX7k[Y51KGenbnXzJIJ6KE63dHzpck0{ Mnr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{C1OFAoRjJ|NEewOVQxRC:jPh?=
ARN8  MmjOSpVv[3Srb36gRZN{[Xl? MVKwMlA2NTJizszN NUnNb4ZoOjRiaB?= MkfadoVlfWOnczD0bIUhcW6mdXP0bY9vKG:oIIC1N{1l\XCnbnTlcpQh\2WwZYOgZpkhVnW2bHnuMVM> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR5MEW0NEc,OjN2N{C1OFA9N2F-
U373 MlrGSpVv[3Srb36gRZN{[Xl? NVP4TFd4OC5{NT:wMlUwOSEQvF2= MVWyOEBp NIXiN4ZqdmO{ZXHz[ZMhWFJvQjDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFVCgmGmQx?= M4mzbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEe0NVcyLz5{M{S3OFE4OTxxYU6=
HASMCs M3vvZmZ2dmO2aX;uJGF{e2G7 NEfpcJQxNTVyMDDuUS=> NUHEN3pCPiCm MXjkc5dvNXKnZ4XsZZRmeyC2aHWg[ZhxemW|c3nvckBw\iCKQVTDJIFv\CClZXzsJJZq[WKrbHn0fS=> M1XJUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUG4OFY4Lz5{M{WxPFQ3PzxxYU6=
MFE296 NGTpSmFHfW6ldHnvckBCe3OjeR?= NGLpSGY2ODBibl2= MlzIOUBl NESyWVVFVVOR NGPlb5hqdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= MoLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ|NUOwO|Y6RC:jPh?=
EN1 NYnqZ|RCTnWwY4Tpc44hSXO|YYm= M{\D[FUxOCCwTR?= NYL0W4psPSCm M{f4fWROW09? NIPObGtqdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= NW[xWoI1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N|A4PjlpPkKzOVMxPzZ7PD;hQi=>
HEC1A M1\uO2Z2dmO2aX;uJGF{e2G7 Mn7qOVAxKG6P MoXaOUBl M{W4RWROW09? M3vDXIlv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF MnrsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ|NUOwO|Y6RC:jPh?=
ECC1 MW\GeY5kfGmxbjDBd5NigQ>? NXLHPHhSPTByIH7N NXzBb3BzPSCm MX\EUXNQ MnnIbY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN? MoXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ|NUOwO|Y6RC:jPh?=
AGS MnPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX[wMlAyPS1zIN88US=> NF;yV2E4OiCq NYHrdnlMcW6mdXPld{Bk\WyuIHTlZZRpKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NY[wc445RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OFUxOjRpPkKzO|Q2ODJ2PD;hQi=>
Huh7 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfPUodWOSEQvF2= M1XYb|I1KGh? MYHEUXNQ M3\QZpJm\HWlZYOgZYJwfXRiMkClJJZq[WKrbHn0feKh MnXmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NEO5N|MoRjJ|NkSzPVM{RC:jPh?=
Lovo M3H2bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljsNE4zNzFwMD:1MlAh|ryP MX[xNk8zPC9|Nj:0PEBp NXrmSHgycW6mdXPld{Bk\WyuIHTlZZRpKGmwIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MlnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5N{CwNFAoRjJ|N{ewNFAxRC:jPh?=
HCT1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\kNE4zNzFwMD:1MlAh|ryP NFfWOWkyOi9{ND:zOk81QCCq Mni3bY5lfWOnczDj[YxtKGSnYYToJIlvKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= M3qwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{ewNFAxLz5{M{e3NFAxODxxYU6=
SUM190PT MoDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoP5OVAwOTByL{K1NEBvVQ>? NVizU4pEPDhiaB?= NWT1[oJ4TE2VTx?= MlLvbY5lfWOnczDj[YxtKGSnYYToJIF1KDVyIH7NJJNmdnOrdHn2[Yx6 NYC2Xpc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI3OzhpPkKzO|kzPjN6PD;hQi=>
SUM149PT NUjieZBsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\CS40zNzdwNT:xNEDPxE1? NGjDXGk1QCCq M1\6NGROW09? NHnzWYxqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6|ZX7zbZRqfmWueR?= M2HOblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOlM5Lz5{M{e5NlY{QDxxYU6=
H1299 NHL4PZBCeG:ydH;zbZMhSXO|YYm= MWmwMlUwOSEQvF2= NHvaOlY1QCCq MWjleIhidm:u MoLGbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? M3\IUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OE[3PVkyLz5{M{i2O|k6OTxxYU6=
A549 M{XIcmFxd3C2b4Ppd{BCe3OjeR?= MV6wMlUwOSEQvF2= NH:xW|U1QCCq M4DCS4V1cGGwb3y= NYPobFZtcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4N{m5NUc,OjN6Nke5PVE9N2F-
H1299 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\XeVAvOjVxMD61M|Eh|ryP NUnZO3VxOjRxNEivO|IhcA>? NIrZSnhmfGijbn;s MkfEbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnuJINwdmOnboTyZZRqd25iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4N{m5NUc,OjN6Nke5PVE9N2F-
A549 MlXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zjb|AvOjVxMD61M|Eh|ryP MY[yOE81QC95MjDo M4\yNIV1cGGwb3y= M33DOIlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDpckBkd26lZX70doF1cW:wIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NGjxdIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i2O|k6OSd-MkO4Olc6QTF:L3G+
H1299 NFTaWnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVuxxsDPxE4EoB?= MVSyOE81QC95MjDo NYK5fHRz\XSqYX7vcC=> M3[wcYlv[3KnYYPld{Bk\WyuIHTlZZRpKGGodHXyJFI1cA>? MnfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MU[2NFkoRjJ|OUG2OlA6RC:jPh?=
PMNs NGLyR3lHfW6ldHnvckBCe3OjeR?= NXr2boo6OzBibl2= NH7LdIY1QCCq M2S1fIlvcGmkaYTzJIVn\mW{b3P5eI9{cXNuIFjERWMh[WO2aY\peJkh[W6mIGLhZ{Bi[3Srdnn0fS=> NI\DNI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m4PFYyPyd-MkO5PFg3OTd:L3G+
CA46 NVjCWoZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPwRmE{NzZxMUKvNlQwPDhibl2= Ml\2OFghcA>? NIHqdGlqdmirYnn0d{B1cGViY3XscEBxem:uaX\ldoF1cW:wIHH0JFI1KGGwZDC0PEBvVcLi MmTxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNkS5OVEoRjJ2ME[0PVUyRC:jPh?=
HCT116 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2r6[lAvOiEQvF2= MnTwNVIhcA>? M13PPYVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgdoFlcWG2aX;u MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF{MkKzNUc,OjRzMkKyN|E9N2F-
HLEB3 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlqxNE41NzBwODFOwG0> MV[0PEBp MmH4SG1UVw>? Mmr5bY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MkPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUe4O|goRjJ2MUW3PFc5RC:jPh?=
SRA01/04  M1rpfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{jzUVAvPC9yLkig{txO MknKOFghcA>? MXTEUXNQ M4XKTIlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O|g9N2F-
HLEB3 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYKwMlIh|ryP NYjPSWdiPDhiaB?= NV2yZVdMTE2VTx?= NXjlfGwxe3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? NFfnbnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1O|g4QCd-MkSxOVc5Pzh:L3G+
SRA01/04  NXLXOXBiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7wUGtyOC5{IN88US=> NYPqZWwzPDhiaB?= NHzoO21FVVOR NUHYUIdDe3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? NWrUOndoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
A2780 M4W1NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2q5XlAvODVvMjFOwG0> M4DOZ|ExNzJ2L{S4JIg> M{XzfGROW09? M4XVWI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NV[2foNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlM5ODFpPkK0NlI{QDBzPD;hQi=>
SKOV3 MmKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17T[VAvODVvMjFOwG0> NX21[|FvOTBxMkSvOFghcA>? MWTEUXNQ NU\4RoRxdWWmaXH0[ZMh\3Kxd4ToJIFzemW|dDDpckBiKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NHLadWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN|gxOSd-MkSyNlM5ODF:L3G+
Huh7  MUPGeY5kfGmxbjDBd5NigQ>? NFjsTY8xNjFxMD61M|EvOCEQvF2= NEHZSIwzPGh? NEDjOHd{fXCycnXzd4V{KFmWSFTDNkBo\W6nIHX4dJJme3Orb36= M{O2VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[5OlczLz5{NEK2PVY4OjxxYU6=
hMSCs M{XYeGZ2dmO2aX;uJGF{e2G7 NF;ZTZQ3NjJ3IH7N NXzkfVQxOjRiaB?= NULsSVBOTE2VTx?= MWDzeIFjcWyrenXzJGhqe3SxbnWgRYNmfHmuYYTpc44h[W6mIITo[UBGgHC{ZYPzbY9vKG:oIGDseZJqeG:2ZX70JGdmdmW| NXLxZ3JIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVI{PTZpPkK0N|EzOzV4PD;hQi=>
HT29  NUH0WYlIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVuxPFAhdk1? NIXMU5M4OiCq NYq3T3ZYUUN3MDDv[kAyQDBibl2= M2m5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[4NlY2Lz5{NEO2PFI3PTxxYU6=
RPE NHP1cYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;jdFAvOi9yLkSvNE45NzFizszN NUDRbWhROjRxNEivO|IhcA>? MV3EUXNQ NVHXOWlWcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44h[nliY3XscEBkgWOuZTDhdpJme3R? NGKwVI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1OlYxOid-MkS0OVY3ODJ:L3G+
U87 GBM M{\ReWZ2dmO2aX;uJGF{e2G7 MVOxNFAwPTByIF7t NF7ve4w1QCCq NWHYfoFLOTByJTDleIhidm:u MV3JcoR2[2W|IGPlcoV{[2WwY3WtUIls\SCDbITldoF1cW:wczDpckBPfWOuZXHyJG1wenCqb3zv[5k> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
U87 GBM M1nEOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHHSoJpOTByL{OwNE82ODBibl2= Mlj3O|IhcA>? NGHtOIgyODBnIHX0bIFvd2x? Mmr6doVlfWOnczDt[YFvKGOnbHygcpVu[mW{IHL5JFMyNCB3NDygZY5lKDV6wrCl MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
ACP03 Mn7LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHVT5czPTBxM{WwM|UxOG6P NFvqXYYzPGh? Mn\v[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>? MmTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Nki1OFcoRjJ2Nk[4OVQ4RC:jPh?=
ACP02 NITvNpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX2yOVAwOzVyL{WwNI5O M{DYTlI1cA>? NX7rWplm\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JI9nKGGycILvfIlu[XSnbImgO|DDqCViYYSgNlUxyqCwTR?= NEjEeXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
HeLa NIHseY5HfW6ldHnvckBCe3OjeR?= NW[yXHlYOW2P NWGzdZJVOC53aB?= NGPSRYhi[m:uaYPo[ZMhfGinIITveIFtKEiGQVOgZYN1cX[rdIm= MmS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MEe0O|QoRjJ2N{C3OFc1RC:jPh?=
HMEC-1  MVfGeY5kfGmxbjDBd5NigQ>? MlrUN|Axdk1? M4HifFI1cA>? M3\kRolv[3KnYYPld{BmgHC{ZYPzbY9vKG:owrDWSWdHWjQEoH3SUmE> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzME[zNUc,OjR5MUC2N|E9N2F-
HeLa  MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjsXZgz|ryP MnjnOFhp MYTk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliYXLveZQhOjVn NHXDS|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0OlE{PSd-MkS4OFYyOzV:L3G+
LNCaP NGXDTJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3OdHcyODBvMUCwNI5O MWOyOIg> MlHpTWM2OCCxZjCzNFBvVQ>? NYfveHBYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVQ3PThpPkK0PFU1PjV6PD;hQi=>
PC3 MnXqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jSZVExOC1zMECwcm0> NF;RUGMzPGh? M3nWTWlEPTBib3[gN|Axdk1? NIrVenc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi1OFY2QCd-MkS4OVQ3PTh:L3G+
CNE2 NFflOZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXGxNFAuPjBybl2= NFrkXFgzPC92OD:3Nog> MV\pcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NILnZoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2PVkxOSd-MkS5Olk6ODF:L3G+
HeLa NHLwOGdHfW6ldHnvckBCe3OjeR?= NFvW[Y4zPTCwTR?= M1zWclE3cA>? MlWxSG1UVw>? MojqbY5kemWjc3XzJGN[WDGDMTDtVm5CKGW6cILld5Nqd28EoB?= NGL6RWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxOlY5QCd-MkWxNVY3QDh:L3G+
A549 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV:wMlAyNTBwOd88US=> NGjaS|Q4Omh? MXLEUXNQ MY\JR|UxKG:oIECuNFbPxE1? Mn\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
SKOV-3 MkDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TEfFAvODFvMD65{txO NX6yfGJ6PzKq M4jDSmROW09? NYTYb20{UUN3MDDv[kAxNjN{zszN M1TCVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
A549 MlP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PiUFAvOS1zzszN NUjabFR3PDiq MnfuSG1UVw>? MoDmTWM2OCCxZjCwMlI5|ryP M1[1elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
SKOV-3 M2PSe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\WNE4yNTIQvF2= MVq0PIg> M1fjbWROW09? MkTXTWM2OCCxZjCwMlfPxE1? NVzpWW9bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
A549 NGrpWVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIKyfI4yNTFyzszN MWeyOIg> NYn5ZnpWTE2VTx?= NF;UZ|dKSzVyIH;mJFMvOs7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
SKOV-3 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX6xMVEx|ryP MnK5NlRp M4TMc2ROW09? MYXJR|UxKG:oIEWuOu69VQ>? NIPEWIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
MCF7 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL1S4hYOC14MEDuUS=> Mn[5NlRp NF\V[XRFVVOR NF3aWYNKSzVyIH;mJFc2dk1? Ml\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUK3NlEoRjJ3MUmyO|IyRC:jPh?=
MDA-MB-231 Mk\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX6wMVYxOG6P MX6yOIg> NXzDV2hFTE2VTx?= MY\JR|UxKG:oIEGwNI5O NYTXUGh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPVI4OjFpPkK1NVkzPzJzPD;hQi=>
A431 NVXZNnNoTnWwY4Tpc44hSXO|YYm= MYO1NI5O NWey[Ih{Oi94L{GyM|I1cA>? NVLhU2NuTE2VTx?= NEDsOG5i[3SrdnH0[ZMheDJzIHHu[EBqdmirYnn0d{BCXEZ|IHX4dJJme3Orb36= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5MUC2PUc,OjV|N{GwOlk9N2F-
A431 M4\sXmFxd3C2b4Ppd{BCe3OjeR?= M1vkR|IwOTBxNUCvNVAxdk1? NUfqOlB4PDiq MlyxSG1UVw>? MXnpcohq[mm2czD0bIUh[2WubDDndo94fGh? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5MUC2PUc,OjV|N{GwOlk9N2F-
PC3  NH\2RnlHfW6ldHnvckBCe3OjeR?= NE\QbnUxNjIQvF2= M1XIXFQ5cA>? MVzEUXNQ NWXGUoh7cW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?= Mo\SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
SW480  MlSzSpVv[3Srb36gRZN{[Xl? MlXiNE4y|ryP MV20PIg> M2r4OGROW09? MVjpcoR2[2W|IHnuZ5Jm[XOnIH;mJGhFSUNzIHHu[EBJTEGFMjDvckBUdHWpIHflcoV{KHC{b33veIVz Mn3pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
PC3  NHHZ[VVHfW6ldHnvckBCe3OjeR?= NYXJPWhjOC5zzszN NGTJZ|A1QGh? MV3EUXNQ MnnEZZR1\W63YYTld{Bqdn[jc3nvckBidmRibXnndoF1cW:w MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|NEm5O{c,OjV2M{S5PVc9N2F-
PC3  MYjGeY5kfGmxbjDBd5NigQ>? MUSwMlHPxE1? NGiz[4c1QGh? Mmf4SG1UVw>? MoHTdoV3\XK|ZYOgSW1V NVfOPVN{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480  M2rLTWZ2dmO2aX;uJGF{e2G7 MUCwMlHPxE1? M4rnd|Q5cA>? NF;EO|hFVVOR MYfheJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|NEm5O{c,OjV2M{S5PVc9N2F-
SW480  NEjy[GJHfW6ldHnvckBCe3OjeR?= M1XSUFAvOc7:TR?= M4\KfFQ5cA>? Mnn4SG1UVw>? NXfhTZhNemW4ZYLz[ZMhTU2W MkLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
MEFs NX7lSpRLTnWwY4Tpc44hSXO|YYm= M4jxSVXPxE1? M2nH[|E3cA>? NUjTU3FXcW6lcnXhd4V{KHSqZTDFVGVEKGG2dHHjbI1mdnRuIGTpdkBl\WyrdnXyfUBidmRidHjlJIVn\mmlaXXuZ5khd2ZicHXk[ZN1[WxiZn;ycYF1cW:w MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6Mk[zOEc,OjV2OEK2N|Q9N2F-
TE13 NILPWIdCeG:ydH;zbZMhSXO|YYm= NGi2dnkxNjQQvF2= NYHhOVRuOjSq M37J[4lvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25? MmnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3N{m2OlUoRjJ3NUe5OlY2RC:jPh?=
TE13 M2qxTGZ2dmO2aX;uJGF{e2G7 NXvUfWlbOC5|zszN MmLqNlRp MX\1dE1z\We3bHH0[ZMhWkGVU1[1RUBt\X[nbB?= M2HVXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUe5OlY2Lz5{NUW3PVY3PTxxYU6=
HEK 293 MVPGeY5kfGmxbjDBd5NigQ>? NF;1WIExNjgQvF2= NGPEUFAzPGh? MUTleIhidm:u NWPaeFVX\W6qYX7j[ZMhTU6jQzDhZ4V1gWyjdHnvckBidmRiaX7jdoVie2W|IFXOZWMh[WK3bnThcoNmKGmwIITo[UB1d3SjbDDj[YxtKGy7c3H0[UBidmRiYYSgeIhmKGOnbHygd5Vz\mGlZR?= MnPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5OEewO|koRjJ3N{i3NFc6RC:jPh?=
MDA-MB-231 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGH1SI0zPS12MECgcm0> MXS0PEBp MV\JR|UxKG:oIEK2N{4zdk4EoB?= M1zaXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEW1NVk5Lz5{M{C1OVE6QDxxYU6=
MCF-7  M1jkZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjUNlUuPDByIH7N NVnwcopRPDhiaB?= NVWyfWhmUUN3MDDv[kAzOjBwNH7N NFmzfY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C1OVE6QCd-MkOwOVUyQTh:L3G+
ECC-1  NHHVXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVOxNFAhdk1? Mn\JNlQhcA>? MYXleIhidm:u NHLBSFhqdmO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh[XCxcITveIlkKG63Y3zlbUB1dyB|NTW= M3Gwc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEK4PFA{Lz5{M{CyPFgxOzxxYU6=
HEC-1A M4rvbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPLbG4yODBibl2= MUeyOEBp NVvqc|Fq\XSqYX7vcC=> MnL3bY5kemWjc3XzJJRp\SCycn;wc5J1cW:wIH;mJIFxd3C2b4TpZ{BvfWOuZXmgeI8hOzln M4n5flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEK4PFA{Lz5{M{CyPFgxOzxxYU6=
NHAC-kn M4XkeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3uV2VbOTBxMUCwM|UxOCCwTR?= M4fwSVEzKGh? M3zHT2ROW09? M1XVNWlEPTBib3[gOVAxdk1? NV;vNpJtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVc5PzFpPkKzNFE4QDdzPD;hQi=>
A549 M1fDfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEfPU4wzPTBibl2= NVrZR5pKPi15MjDo NGPQTVNFVVOR MkHSZ4F2e2W|IHGg[5Jm[XSncjDpcohq[mm2b4L5JIVn\mWldDDjc41jcW6nIIfpeIghXFiWIH;yJIVzdG:2aX7pZi=> NEX0[Y89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm5OFc5OCd-MkK5PVQ4QDB:L3G+
MG-63 MnjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfKXVE{ODBibl2= M37t[|EzKGh? NXnYWpAzTE2VTx?= NGTZSHJqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hQDZn Mn\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5OUmzN|goRjJ{N{m5N|M5RC:jPh?=
MG-63 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\ZeVY{ODBibl2= MmTsNlQhcA>? NEL1W5RFVVOR MYXpcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gOlcm M2HFWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{m5N|M5Lz5{Mke5PVM{QDxxYU6=
MG-63 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3DTHk{ODBibl2= MVW0PEBp MVPEUXNQ NELSelRqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPTZn MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd7OUOzPEc,OjJ5OUmzN|g9N2F-
HL60  MlXMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYX4co01OTVyLUO1NEBvVQ>? MUOyOEBp MYPpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXQEoIfo[Y4h[2:wY3XueJJifGmxboOgbIlocGW{IIToZY4hOjVyIH7N MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3M{ezPUc,OjJ5NUO3N|k9N2F-
U937 M1Tae2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVe0TZNROTVyLUO1NEBvVQ>? NH3lbIwzPCCq NIG3fI1qdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3M{ezPUc,OjJ5NUO3N|k9N2F-
SCC-6 NX\ueoNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVSyNFAuOzJyMDDuUS=> NH23[HoyOi9{ND:0PEBp NFr1SnlFVVOR NYX0NY9KcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hd2ZiU1PDMVYh[2WubIOgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NFjSdlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW1NlMzOSd-MkK1OVI{OjF:L3G+
U87  Mmq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzaNFkyODBvM{CwJI5o NF\lSHYzPCCq NGS3N2tqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPzJnIDDheEAzODCwZx?= NUW4cWhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyO|A5PDlpPkKyNlcxQDR7PD;hQi=>
K562 M3yyc2Z2dmO2aX;uJGF{e2G7 NFnlSYEyKM7:TR?= MmXFNVIhcA>? NFjnWHdFVVOR MU\lcohidmOnczD0bIUh\XiycnXzd4lwdiCxZjDSWW5ZOyCrbnT1Z4VlKGK7IEWtZZpiNUOmUh?= NXjXNopPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxO|kyQThpPkKyNVc6OTl6PD;hQi=>
Reh M1zufmZ2dmO2aX;uJGF{e2G7 MWmwMlMwOSEQvF2= NH63cngyOiCq MlriSG1UVw>? NITUNFRmdmijbnPld{B1cGViZYjwdoV{e2mxbjDv[kBTXU6[MzDpcoR2[2WmIHL5JFUu[XqjLVPkVi=> M1PMS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUe5NVk5Lz5{MkG3PVE6QDxxYU6=
Mv1Lu MYXGeY5kfGmxbjDhd5NigQ>? NID3dHkyQCCqcoO= NXyz[GhiUW6mdXP0bY9vKG:oIIfpcIQufHmyZTDwNlEheHKxbX;0[ZIh[WO2aY\heIlwdiCrbjDtbY5sKE24MVz1JINmdGy|IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6KHKnbHH0bZZmKHSxIHLhd4FtKGyndnXsMEBGSzFyMECgQUAxNjF7IN88UU4> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh6MUKzNkc,OTd6OEGyN|I9N2F-
HeLa NHTLNGNHfW6ldHnvckBie3OjeR?= MlLLNlQhcHK| NI\EWWVKdmS3Y4Tpc44hd2ZicEKxJIV5eHKnc4Ppc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjZoTldkAzPCCqcoOsJGVEOTByMDC9JFAvODFzNTFOwG0v M{HYUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{m3PFI3Lz5zOEO5O|gzPjxxYU6=
Mv1Lu NFHYcXhHfW6ldHnvckBie3OjeR?= NEnwdZozPCCqcoO= NYTlZnhFUW6lcnXhd4UhcW5iaIXtZY4hf2muZDD0fZBmKHB{MTDwdo91\WmwIHX4dJJme3Orb36gbY4hdWmwazDNelFNfSClZXzsd{Bi\nSncjCyOEBpenNiYomgdFIyKHC{b33veIVzKGG|c3H5MEBGSzFyMECgQUAxNjB{IN88UU4> M4rkUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm3OVc5Lz5{NEm5O|U4QDxxYU6=
HCT 116 NFrO[VFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3[x[mlvKH[rdILvJIFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iY3;sc44h[2GwY3XyJGhEXCBzMU[gZ4VtdHNiZHX0[ZJucW6nZDDifUBOVVRiYYPzZZktKEWFNUCgQUAyKM7:TT6= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV7N{SxN{c,OTF3OUe0NVM9N2F-
T24 NF;zT5VHfW6ldHnvckBie3OjeR?= NYjhd2ExS2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBz\XG3aYLl[EBnd3JiYXPleJlt[XSrb36gc4YhcGm|dH;u[U01KGmwIHj1cYFvKFR{NDDjZY5k\XJiY3XscJMtKEWFNUCgQUAyKM7:TT6= NEfWWmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5O|QyOyd-MUG1PVc1OTN:L3G+
T24 MWrGeY5kfGmxbjDhd5NigQ>? M{nRfmlvcGmkaYTpc44hd2ZiYXPleJlt[XSrb36gc4YhcGm|dH;u[U01KGmwIHj1cYFvKFRvMkSgZ4Fv[2W{IHPlcIx{NCCHQ{WwJF0hOSEQvF2u NGDMXWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNE[xN|MyOid-MUS2NVM{OTJ:L3G+
T24 MVfGeY5kfGmxbjDhd5NigQ>? NWjiXYQzUW6qaXLpeIlwdiCxZjDhZ4V1gWyjdHnvckBw\iCqaYP0c45mNTRiaX6gbJVu[W5iVD2yOEBk[W6lZYKgZ4VtdHNuIFXDOVAhRSB2IN88UU4> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDZzM{OxNkc,OTR4MUOzNVI9N2F-
T24 MV7GeY5kfGmxbjDhd5NigQ>? NFSxRZZKdmS3Y4Tpc44hd2ZiYXzwbIEhfHWkdXzpckBi[2W2eXzheIlwdiCrbjDoeY1idiCWMkSgZ4VtdHNuIFXDOVAhRSByLkCxJO69VS5? M2TJcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUGxOFY3Lz5zOUGxNVQ3PjxxYU6=
T24 Ml24SpVv[3Srb36gZZN{[Xl? NHHBW3dKdmS3Y4Tpc44hd2ZiaHnzeI9v\SCKMzDhZ4V1gWyjdHnvckBqdiCqdX3hckBVOjRiY3XscJMtKEWFNUCgQUAxNjBzIN88UU4> M4\BTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUGxOFY3Lz5zOUGxNVQ3PjxxYU6=
HepG2 Mlz2SpVv[3Srb36gZZN{[Xl? M{PlOXVxNXKnZ4XsZZRqd25ib3[geJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IH;mJIh2dWGwIFPMRU0yKHC{b33veIVzKHKnZ3nvckAuOTB3NTD0c{AuPjKkcDD0doFve2[nY4Tl[EBqdiCKZYDHNkBk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO4JO69VS5? M4fSNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW2NVAzLz5{NUW1OlExOjxxYU6=
HeLa MYDGeY5kfGmxbjDhd5NigQ>? MUXJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB1fWK3bHnuJIRm[WOndInsZZRqd25iaX7jeYJifGWmIH\vdkBwfmW{bnnnbJQh[nliY1XMTXNCNCCHQ{WwJF0hOC5yMEWyJO69VS5? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzMUmxPUc,OjZ4MUG5NVk9N2F-
HL60 NH;zfoZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4DW[lI1KGi{cx?= NUniW29iSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGdKPTBiPTCwMlU6KM7:TT6= NHPMXFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS1OFkyQCd-Mkm0OVQ6OTh:L3G+
MDA-MB-435 NEHhXZhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MnK1NlQhcHK| NFv3UINCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi12M{WgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDFwN{ig{txONg>? Mn\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NUS5NVgoRjJ7NEW0PVE5RC:jPh?=
K562 Mn;6SpVv[3Srb36gZZN{[Xl? MV;Jcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgdIFzfGmjbHz5JJB2emmoaXXkJIhqe3SxbnWg[IVi[2W2eXzhd4Uhd2ZiaIXtZY4hdGW3a3XtbYEhUzV4MjDj[Yxtey5uIFnDOVAhRSByLkCwOFYh|ryPLh?= NUnHT4JYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0NlUyOTBpPkGwOFI2OTFyPD;hQi=>
A20 NHrmbFdHfW6ldHnvckBie3OjeR?= MWLJckB3cXS{bzDmc5Ih[W62aT3ISGFEOSBqSHnzeI9v\SCmZXHj[ZR6dGG|ZTCxLUBi[3Srdnn0fUBqdiCvb4Xz[UBCOjBiY3XscJMtKEmFNUCgQUAxNjByMjFOwG0v NHu1NJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUm2NFQ5QSd-MUG5OlA1QDl:L3G+
K 562 MY\GeY5kfGmxbjDhd5NigQ>? NHzwVnBKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUGm|dH;u[UBl\WGlZYT5cIF{\SBqSFTBR{khcW5iSzC1OlIh\XK7dHjyc4xmfWunbXnhJINmdGy|LDDJR|UxKD1iMD6wNFM1KM7:TT6= NIX5SXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEW5NlQ4Oyd-MUS1PVI1PzN:L3G+
friend cell M2TMd2Z2dmO2aX;uJIF{e2G7 MXHJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCocnnlcoQh[2WubIOgdJJwdGmoZYLheIlwdixiSVO1NEA:KDBwMESg{txONg>? M2W3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NkGzN|EzLz5zNE[xN|MyOjxxYU6=
SNU-16 Mm\CSpVv[3Srb36gZZN{[Xl? NH:ySmpKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUGm|dH;u[UBl\WGlZYT5cIF{\SBqSFTBR{kh\nKxbTDTUnUuOTZiKHj1cYFvKGejc4TybYMh[WSnbn;jZZJkcW6xbXGpJINmdGy|LDDJR|UxKD1iMD6wOVMzQSEQvF2u M{PlVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2Nk[3NlI4Lz5zNE[2O|IzPzxxYU6=
melanoma B16/BL6 M1q0bGZ2dmO2aX;uJIF{e2G7 MYLJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbIl{fG:wZTDk[YFk\XS7bHHz[ZMhMEiGQVOxLUBxemWyYYLl[EBnem:vIH3veZNmKG2nbHHuc41iKEJzNj;CUFYh[2WubIOsJGlEPTBiPTCwMlAyQSEQvF2u M1vkSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MUC5OlI3Lz5zNUGwPVYzPjxxYU6=
melanoma B16/BL6 MVLGeY5kfGmxbjDhd5NigQ>? M{fvdWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKCiKRFHDOEkheHKncHHy[YQh\nKxbTDtc5V{\SCvZXzhco9u[SCEMU[vRmw3KGOnbHzzMEBKSzVyIE2gNE4xOiEQvF2u MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6 NGjuXFdHfW6ldHnvckBie3OjeR?= MoTaTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB{ODFOwG0v NIO1[4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
melanoma B16/BL6 MYDGeY5kfGmxbjDhd5NigQ>? M2D4R2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKDhicILldIFz\WRiZoLvcUBud3W|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yNDFOwG0v MlrNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVzMEm2NlYoRjF3MUC5OlI3RC:jPh?=
293T NYrJWJFXTnWwY4Tpc44h[XO|YYm= NE\ZVVZKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViNDDwdoVx[XKnZDDmdo9uKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCyJO69VS5? NWL5ZlJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
293T MYTGeY5kfGmxbjDhd5NigQ>? M4X2XGlvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SBzIIDy[ZBiemWmIH\yc40hOjl|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>? NVrLTlNuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
293T M{HmfmZ2dmO2aX;uJIF{e2G7 M{nlVmlvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SB4IIDy[ZBiemWmIH\yc40hOjl|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>? M1T2PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEW0NlI1Lz5zNUS1OFIzPDxxYU6=
293T NVexRXJDTnWwY4Tpc44h[XO|YYm= M4XIT2lvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SB6IIDy[ZBiemWmIH\yc40hOjl|VDDj[YxteyxiSVO1NEA:KDBwMESg{txONg>? NUmwT3lWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
HeLa MUPGeY5kfGmxbjDhd5NigQ>? Moq5TY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SBqSFTBR{kh\nKxbTDI[WxiKGOnbHzzJJdqfGhic4Xid5Rz[XSnIEXhMEBKSzVyIE2gNE4xPTR3IN88UU4> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NkK2O{c,OTV2NU[yOlc9N2F-
HCT116 MXTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MoX2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDIR3QyOTZiY3XscJMtKEmFNUCgQUAxNjByMUOg{txONg>? NGXKNGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmwOFg6OCd-MU[5NFQ5QTB:L3G+
H1299 NVX1SHNVSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFixNlk6KGOnbHzzMEBKSzVyIE2gNE4yKM7:TT6= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlyNEi5NEc,OTZ7MES4PVA9N2F-
H661 MmPNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmXoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDIOlYyKGOnbHzzMEBKSzVyIE2gNE4{KM7:TT6= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlyNEi5NEc,OTZ7MES4PVA9N2F-
H661 NVjXSpFNSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NVu4c2hjPDhiaILz NHrqZ3ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGg3PjFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHhVXCCjc4PhfUwhUUN3MDC9JFAvODh3IN88UU4> NY\NdGp6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NlQ4PzNpPkG3OlI1Pzd|PD;hQi=>
WiDr M3r3cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUjTeJp[UW6qaXLpeIlwdiCxZjDTRXAyOzBibXXkbYF1\WRiY3XscEBoem:5dHigbY4hcHWvYX6gW4lFeiClZXzsd{whUUN3MDC9JFAvPTd5NjFOwG0v MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ2M{GxNkc,OTd4NEOxNVI9N2F-
U251 NVnWXW13TnWwY4Tpc44h[XO|YYm= MnnETY5pcWKrdHnvckBw\iCVQWCxN|AhcW5iVlXHSk1{fGmvdXzheIVlKGi3bXHuJHUzPTFiY3XscJMh[nliUFzBVEBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC54MUGyJO69VS5? MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NEOxNVIoRjF5NkSzNVEzRC:jPh?=
293T NYrmVpBqTnWwY4Tpc44h[XO|YYm= MnnmTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDlfJBz\XO|ZXSgbY4hOjl|VDDj[YxteyxiSVO1NEA:KDBwMECxPUDPxE1w M1qxPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T NUeyOJE5TnWwY4Tpc44h[XO|YYm= NYTZWIJ1UW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFAzKM7:TT6= M2nJcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T M1PPSGZ2dmO2aX;uJIF{e2G7 NGf0bVNKdmirYnn0bY9vKG:oIH3veZNmKEiGQVO2JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy|LDDJR|UxKD1iMD6wNFI5KM7:TT6= Ml32QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN|IoRjF5OEixNlMzRC:jPh?=
H661 NVLXVVltSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NITGR2M1QCCqcoO= Mk\sRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDIOlYyKGOnbHzzJIFnfGW{IES4JIhzeyxiSVO1NEA:KDBwMEi1JO69VS5? M2rn[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEm3PFI1Lz5zN{i5O|gzPDxxYU6=
293T NF[zbFhHfW6ldHnvckBie3OjeR?= NVjMSZNQUW6qaXLpeIlwdiCxZjDISGFEOSCneIDy[ZN{\WRiaX6gNlk{XCClZXzsd{whUUN3MDC9JFAvOTlizszNMi=> NHfOSok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
293T M2CyNGZ2dmO2aX;uJIF{e2G7 M1W3ZWlvcGmkaYTpc44hd2ZiSFTBR|Qh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlIh|ryPLh?= NVu2NXpDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T M3vZcmZ2dmO2aX;uJIF{e2G7 MoHSTY5pcWKrdHnvckBw\iCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNlgh|ryPLh?= NVWx[FZ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
NIH3T3 MYXGeY5kfGmxbjDhd5NigQ>? NEnMSYFKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDOTWg{XDNiY3XscJMtKEmFNUCgQUAxNjB{MTFOwG0v MmD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O|k5RC:jPh?=
human 293 NV3iSWZbTnWwY4Tpc44h[XO|YYm= MlPSTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzJINmdGy|LDDJR|UxKD1iMD6wN|Qh|ryPLh?= NULKRoF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Nlk4QThpPkG3PVI6Pzl6PD;hQi=>
mouse 293 NYDtN41VTnWwY4Tpc44h[XO|YYm= NV2wTZhDUW6qaXLpeIlwdiCxZjDtc5V{\SC{ZXPvcYJqdmGwdDDISGFEPiCneIDy[ZN{\WRiaX6gNlk{KGOnbHzzMEBKSzVyIE2gNE4xQDFizszNMi=> M{DZNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUK5O|k5Lz5zN{myPVc6QDxxYU6=
SKBR3 M1ixUWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NYfhPZBzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT2JTOyClZXzsd{whUUN3MDC9JFAvODJizszNMi=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L NIS0PVlHfW6ldHnvckBie3OjeR?= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJG1OQT[OIHPlcIx{NCCLQ{WwJF0hOC5yMzFOwG0v NGjlSVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O|U2PCd-MUiyOFc2PTR:L3G+
SNU16 NXi0bFk{TnWwY4Tpc44h[XO|YYm= M1TVXmlvcGmkaYTpc44hd2ZiSFTBR{Bnem:vIHj1cYFvKFOQVUG2JINmdGy|LDDJR|UxKD1iMD6wOVMh|ryPLh?= NIWwTHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O|U2PCd-MUiyOFc2PTR:L3G+
A549 NH\2dGtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MkXORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|LDDJR|UxKD1iMD6wPEDPxE1w MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MKN45 MWfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NEPBTFZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3LUlQ2KGOnbHzzMEBKSzVyIE2gNE4yKM7:TT6= NHy5VFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O|U2PCd-MUiyOFc2PTR:L3G+
SQ20B M4jjfmZ2dmO2aX;uJIF{e2G7 MWjJcohq[mm2aX;uJI9nKGi3bXHuJHNSOjCEIHPlcIx{NCCLQ{WwJF0hOC5{IN88UU4> M4HRN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O|U2PDxxYU6=
neonatal foreskin fibroblasts MmLCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWXadZl7T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hdmWxbnH0ZYwh\m:{ZYPrbY4h\mmkcn;icIF{fHNiY3XscJMtKEmFNUCgQUAxNjJizszNMi=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
HeLa NHWx[ZFHfW6ldHnvckBie3OjeR?= MoLjTY5pcWKrdHnvckBw\iCKRFHDJIZzd21iaIXtZY4hUGWOYTDj[YxteyxiSVO1NEA:KDBwMzFOwG0v MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L/NFF NEjPXWpHfW6ldHnvckBie3OjeR?= NWTMXmRwW2WuZXP0bZZqfHliaX7k[ZgtKHKjdHnvJI9nKEmFNUCg[o9zKGi3bXHuJG1OQT[OIHPlcIx{KHSxIFnDOVAh\m:{IHj1cYFvKE6IRjDj[YxteyxiSVO1NEA:KDZwNzFOwG0v MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
293T MoflSpVv[3Srb36gZZN{[Xl? M3WwTmlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Gg[ZhxemW|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCzOlYh|ryPLh?= M3HuclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{m3PFI3Lz5zOEO5O|gzPjxxYU6=
293T MW\GeY5kfGmxbjDhd5NigQ>? M1juOmlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Sg[ZhxemW|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkC2Nlkh|ryPLh?= NGfxVWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O|gzPid-MUizPVc5OjZ:L3G+
293T NXfEenR3TnWwY4Tpc44h[XO|YYm= NYXCRlZJUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFg{OyEQvF2u NE\DO5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O|gzPid-MUizPVc5OjZ:L3G+
HeLa NHTTR|NHfW6ldHnvckBie3OjeR?= MnnQTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFIHnuJGhmVGFiY3XscJMh[nliZnzveZIh\GVibInzJIF{e2G7LDDJR|UxKD1iMD6wNFUh|ryPLh?= NXPYcHlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVc5OjdpPkG4N|k4QDJ5PD;hQi=>
S2 MUTGeY5kfGmxbjDhd5NigQ>? MkLPTY5pcWKrdHnvckBw\iCSbHHzcY9lcXWvIH\hcINqeGG{dX2gTGRCSzFiZYjwdoV{e2WmIHnuJGRzd3OxcHjpcIEhdWWuYX7v[4F{fGW{IGOyJINmdGy|LDDJR|UxKD1iMD6wNFA3KM7:TT6= NETzZY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOxO|Q2OCd-MUmzNVc1PTB:L3G+
NCI-H661 NXHjNodsSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MkLrOFghcHK| NFrKcllCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTU1JPjZzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjFizszNMi=> MljyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|OEW2NFAoRjF7M{i1OlAxRC:jPh?=
NIH3T3 MoLGSpVv[3Srb36gZZN{[Xl? M4jJemlvcGmkaYTpc44hd2ZiSFTBR|EhcW5iaIXtZY4hVkmKM2SzJINmdGy|IHL5JJJi\GmxbXX0dolkKGirc4TvcoUh\GWjY3X0fYxifGmxbjDhd5NigSxiSVO1NEA:KDBwMEC2JO69VS5? M4Kz[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEW3OlU6Lz5zOUS1O|Y2QTxxYU6=
HeLa MkPmSpVv[3Srb36gZZN{[Xl? MXfJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBJ\UyjIHPlcIx{KGK7IH\seY9zd2enbnnjJIVvgnmvZTDhd5NigSxiSVO1NEA:KDBwMzFOwG0v NXmyXphsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVc3PTlpPkG5OFU4PjV7PD;hQi=>
ML1 NGP3RnVCdnSrdIXtc5Ih[XO|YYm= NVm0OHA4SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhdW:3c3WgUWwyKGOnbHzzMEBKSzVyIE2gNE4zPSEQvF2u NIfaWFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWzOFU{PCd-MUm1N|Q2OzR:L3G+
HCT116 NInjZlJEgXSxdH;4bYNqfHliYYPzZZk> NVTmOplXOyCmYYnz NF7aWpJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhOyCmYYnzJIJ6KFeVVD2xJIF{e2G7LDDJR|UxKD1iMD64JO69VS5? NFjvOFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPid-MkC0OVIzOjZ:L3G+
HCT116 NHvpd2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYXSWFlzPDhiaILz MYrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvODR|IN88UU4> NEHRbI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC3N|E3OCd-MkGwO|MyPjB:L3G+
H460 Mk\lS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlPyOFghcHK| NX;E[XF4T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUDR4MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5zIN88UU4> NIT0cW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC3N|E3OCd-MkGwO|MyPjB:L3G+
DMS53 MmPDR5l1d3SxeHnjbZR6KGG|c3H5 NIHOdoszKGSjeYO= NX3DXJh3S3m2b4P0ZZRq[yCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGROWzV|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB{NTFOwG0v NIfnVGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWwOFIyPCd-MkG1NFQzOTR:L3G+
HeLa NX7nRZhuTnWwY4Tpc44h[XO|YYm= M4DNVFIxKG2rboO= NHjP[HFKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDj[YxteyC3c3nu[{BC[y2DcnetS4x6NUy7czjBZ{kuSU2FIHHzJIZtfW:{ZYPj[Y51KHO3YoP0doF1\SCjZoTldkAzOCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMECzJO69VS5? Mli2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4MkG4PFMoRjJzNkKxPFg{RC:jPh?=
Sf9 MU\GeY5kfGmxbjDhd5NigQ>? NF7mWlAyKGi{ MYLJcohq[mm2aX;uJI9nKE5idHXycYlv[WxiaHX4ZYhqe3SrZHnu[U11[WepZXSgbJVu[W5iSFTBR|gh\XiycnXzd4VlKGmwIGPmPUBk\WyuczDh[pRmeiBzIHjyJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwOU[g{txONg>? NGrueGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUeyN|c{Oyd-MkG3NlM4OzN:L3G+
MCF7 M{nCcWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NYrJdHpIPDhiaILz NHvGVGNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuO{DPxE1w Mnv1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkWwPVEoRjJ{NE[1NFkyRC:jPh?=
BE(2)-C NWq3bHluSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVy3NkBpenN? Mm\pRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCERTiyLU1EKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDBcIFu[XJiYnz1[UBie3OjeTygTWM2OCB;IECuNFUh|ryPLh?= M2\IWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUOyN|E3Lz5{MkmzNlMyPjxxYU6=
Sf9 MnP0SpVv[3Srb36gZZN{[Xl? Ml;kNkBpenN? MYXJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JIV5eHKnc4Pl[EBqdiCVZkmgZ4VtdHNiaX7jeYJifGWmIH\vdkAzKGi{czD1d4lv\yCUSFvLMWFkKG[udX;yc4dmdmmlIIP1ZpN1emG2ZTygTWM2OCB;IECuNFAyOiEQvF2u M4LzRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEC5NlA{Lz5{M{CwPVIxOzxxYU6=
Sf9 MXnGeY5kfGmxbjDhd5NigQ>? MWKyJIhzew>? NH;MZ|VKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgbY5kfWKjdHXkJIZweiB{IHjyd{B2e2mwZzDSTGtMNUGlIH\seY9zd2enbnnjJJN2[nO2cnH0[UwhUUN3MDC9JFAvODB3IN88UU4> NXTjUmt6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFkzODNpPkKzNFA6OjB|PD;hQi=>
A2780 MWrDfZRwfG:6aXPpeJkh[XO|YYm= M4nDN|czKGi{cx?= MkjmR5l1d3SxeHnjbZR6KGGpYXnud5Qh[2m|cHzheIlvKHKnc3nzeIFvfCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{MjFOwG0v NH\jWVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
A2780 MXHGeY5kfGmxbjDhd5NigQ>? NFW2VI0yQCCqcoO= Mof4TY5pcWKrdHnvckBw\iCKRFHDJIlvKGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMkWg{txONg>? MnO3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
A2780 NHTNV3NEgXSxdH;4bYNqfHliYYPzZZk> MX:3NkBpenN? M4fybWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ7IN88UU4> NYTDPYNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
A2780 MXvGeY5kfGmxbjDhd5NigQ>? NIHBVGIyQCCqcoO= MmXCTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFE5KGi{czDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjR|IN88UU4> MmDGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
Sf9 MlviSpVv[3Srb36gZZN{[Xl? MmW2TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOkBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOvd4Y6KGOnbHzzJJV{cW6pIGLIT2tC[yCjczDzeYJ{fHKjdHWsJGlEPTBiPTCwMlAxODlizszNMi=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
Sf9 NILXNFBHfW6ldHnvckBie3OjeR?= NYXBXHhKUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMwe2Z7IHPlcIx{KHW|aX7nJHJJU0uDYzDhd{B{fWK|dILheIUtKEmFNUCgQUAxNjByNTFOwG0v M{DCOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
Sf9 Mn\uSpVv[3Srb36gZZN{[Xl? M1\D[mlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|Ih\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzM5NnQSClZXzsd{B2e2mwZzDSTGtMSWNiYYOgd5Vje3S{YYTlMEBKSzVyIE2gNE4xODVizszNMi=> NFj6cFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
Sf9 NInqSohHfW6ldHnvckBie3OjeR?= MknsTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDNVAh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzM5NnQSClZXzsd{B2e2mwZzDSTGtMSWNiYYOgd5Vje3S{YYTlMEBKSzVyIE2gNE4xODlizszNMi=> NWrlOm5HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
Sf9 MXnGeY5kfGmxbjDhd5NigQ>? MXvJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxNUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOvd4Y6KGOnbHzzJJV{cW6pIGLIT2tC[yCjczDzeYJ{fHKjdHWsJGlEPTBiPTCwMlAyPzNizszNMi=> MkK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
Sf9 M3HpNmZ2dmO2aX;uJIF{e2G7 MnP1TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDPEBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOvd4Y6KGOnbHzzJJV{cW6pIGLIT2FkU0GlIHHzJJN2[nO2cnH0[UwhUUN3MDC9JFAvOTJ7IN88UU4> M3HwRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
MOLT4 MUjDfZRwfG:6aXPpeJkh[XO|YYm= MVy3NkBpenN? MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNU2xVPCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkCzJO69VS5? NGnIb3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
A549 M1e4WWN6fG:2b4jpZ4l1gSCjc4PhfS=> NFTUS2U4OiCqcoO= MlLNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkC1JO69VS5? NU[5OVdzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
MCF7 NYPxendmS3m2b4TvfIlkcXS7IHHzd4F6 NYrzR5JlPzJiaILz NFLzWJhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuNFYh|ryPLh?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4ME[yO{c,OjR7NkC2Nlc9N2F-
HL60 NXHxW49CS3m2b4TvfIlkcXS7IHHzd4F6 MVi3NkBpenN? M3O1SmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFQ1u4JIF{e2G7LDDJR|UxKD1iMD6wPEDPxE1w NGD6eWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
BGC823 M2[0[WN6fG:2b4jpZ4l1gSCjc4PhfS=> MVW3NkBpenN? Ml\GR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuNFgh|ryPLh?= Mlm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
HuH7 NIDYeFNEgXSxdH;4bYNqfHliYYPzZZk> NYnDRmMyPzJiaILz MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIeWg4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhS0ONODDhd5NigSxiSVO1NEA:KDBwMTFOwG0v M1PhWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wOlI4Lz5{NEm2NFYzPzxxYU6=
U937 M4HnWmN6fG:2b4jpZ4l1gSCjc4PhfS=> M33DWFczKGi{cx?= MmLPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVk{PyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkGg{txONg>? NXHEbnhsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
HeLa MlXLR5l1d3SxeHnjbZR6KGG|c3H5 M4Xk[|czKGi{cx?= MoPlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkGxJO69VS5? NFP2PW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
NCI-H1975 MmrOR5l1d3SxeHnjbZR6KGG|c3H5 NYTCbZZYPzJiaILz NEThW4ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSEG5O|Uh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBES0t6IHHzd4F6NCCLQ{WwJF0hOC5{MTFOwG0v Mn\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
K562 M{LFXmN6fG:2b4jpZ4l1gSCjc4PhfS=> NU\VWFJiPzJiaILz NIXZcINEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuOFEh|ryPLh?= NWP2Ooo6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
HEK293 MX7DfZRwfG:6aXPpeJkh[XO|YYm= M37iS|k3KGi{cx?= NUXGd5FuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEWNMkmzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkG2JO69VS5? MnHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NU[xNFIoRjJ3NUW2NVAzRC:jPh?=
LO2 MXzDfZRwfG:6aXPpeJkh[XO|YYm= NVy3VoE2QTZiaILz M1exN2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxQOiClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4zOSEQvF2u M3m2eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW2NVAzLz5{NUW1OlExOjxxYU6=
HeLa NIr1dWNEgXSxdH;4bYNqfHliYYPzZZk> MUW5OkBpenN? MkHNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE45PSEQvF2u MoHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NU[xNFIoRjJ3NUW2NVAzRC:jPh?=
HepG2 M2[5cWN6fG:2b4jpZ4l1gSCjc4PhfS=> MX:5OkBpenN? MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE46PiEQvF2u NI\SXYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OlExOid-MkW1OVYyODJ:L3G+
U2OS NYfQSodES3m2b4TvfIlkcXS7IHHzd4F6 NVnJXFhqQTZiaILz M{XiR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHUzV1NiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFE1Njl|IN88UU4> MmnaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NU[xNFIoRjJ3NUW2NVAzRC:jPh?=
Sf9 MV\GeY5kfGmxbjDhd5NigQ>? NFvVSnI6OCCvaX7z MV\Jcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IF6teIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFNjCoNUB1dyBzMkG1JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5ic3[5JINmdGy|IIXzbY5oKFKKSz3LLGFkMS2DTVOgZZMhe3Wkc4TyZZRmKGmwY4XiZZRm\CCob4KgPVAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjByMUexOkDPxE1w M1LGWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO|Y1Lz5{N{C2NFc3PDxxYU6=
Sf21 NIryc4NHfW6ldHnvckBie3OjeR?= M2Lt[FYxKG2rboO= MoPoTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGZNSUdvSHnzMZRi\2enZDDISGFEOSBqMTD0c{A1QDJicnXzbYR2\XNrIHX4dJJme3OnZDDpckBU\jJzIHPlcIx{KHW|aX7nJHJJUy2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIlv[3WkYYTl[EBnd3JiNkCgcYlveyCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCwMlAxPjV5NDFOwG0v NY\ZNY5XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
insect cell M2nte2Z2dmO2aX;uJIF{e2G7 MYO2NEBucW6| NEXEN3pKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIILlZ49u[mmwYX70JGMufGW{bXnuZYwhT1OWLYTh[4dm\CCKRFHDNkApOSC2bzC0PFghemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMhfXOrbnegVmhMNUtqQXOpMWFOSyCjczDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB4MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFExPTFizszNMi=> NWXDPHozRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
insect cell NWHHb5NNTnWwY4Tpc44h[XO|YYm= MYe2NEBucW6| MoDHTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{OgLFEhfG9iNEK4JJJme2mmdXXzLU9pfW2jbjDy[YNwdWKrbnHueEBPNXSncn3pcoFtKEeVVD30ZYdo\WRiTlPPVlIh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{B2e2mwZzDSTGsuUyiDYzmtRW1EKGG|IIP1ZpN1emG2ZTDpcoN2[mG2ZXSg[o9zKDZyIH3pcpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6wNVExPyEQvF2u NVHvcYVzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
insect cell NGLqOW9HfW6ldHnvckBie3OjeR?= NHfZV4RKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IF6teIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFMUGgLFEhfG9iM{S3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzM4Ji[3Wub4\pdpV{KGW6cILld5Nqd25ic4nzeIVuKHW|aX7nJHJJUy2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMEGxN|ch|ryPLh?= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
insect cell MUfGeY5kfGmxbjDhd5NigQ>? NWnOUmF2PjBibXnudy=> NYjXUVVZUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{igLFEhfG9iM{e3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3LLGFkMS2DTVOgZZMhe3Wkc4TyZZRmKGmwY4XiZZRm\CCob4KgOlAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjNyN{Sg{txONg>? MnnIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO|Y1RC:jPh?=
SHSY5Y Ml:ySpVv[3Srb36gZZN{[Xl? M1y5fVghcHK| M3fjWGlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hW0iVWUXZJINmdGy|IIXzbY5oKEKDVFPQJIF{KHO3YoP0doF1\SCjZoTldkA5KGi{czDifUBWUFCOQz3FV2kuVVNxTWOgZY5idHm|aYOsJGlEPTBiPTCwMlAxPzhizszNMi=> NILwbWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[1NFkzPSd-Mke2OVA6OjV:L3G+
SHSY5Y MkXlSpVv[3Srb36gZZN{[Xl? Mnf1PEBpenN? NUDlPY1pUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKFOKU2m1XUBk\WyuczD1d4lv\yCPQVygZZMhe3Wkc4TyZZRmKGGodHXyJFghcHK|IHL5JHVJWEyFLVXTTU1OWy:PUzDhcoFtgXOrczygTWM2OCB;IECuNFA6OSEQvF2u NV3zPGxIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
Sf9 NGfURlNHfW6ldHnvckBie3OjeR?= M1LXV|QhcHK| NV3o[Fc3UW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDHV3QufGGpZ3XkJGhFSUNzIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCVZkmgbY5{\WO2IHPlcIx{KHW|aX7nJG1QS1CDQzDhd{B{fWK|dILheIUh[W[2ZYKgOEBpenNiYomgWWhRVENvRWPJMW1UN02VIHHuZYx6e2m|LDDJR|UxKD1iMD6wNVU4KM7:TT6= NX\zcVBURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
insect cell MoG1SpVv[3Srb36gZZN{[Xl? M2Pm[VQhcHK| MWPJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDpcpNm[3RiY3XscJMhfXOrbnegRmFVS1BiYYOgd5Vje3S{YYTlJIFnfGW{IESgbJJ{KGK7IGXIVGxENUWVST3NV{9OWyCjbnHsfZNqeyxiSVO1NEA:KDBwMEG2JO69VS5? MlOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4NUC5NlUoRjJ5NkWwPVI2RC:jPh?=
SHSY5Y MnSwSpVv[3Srb36gZZN{[Xl? MnHDPEBpenN? M17WfGlvcGmkaYTpc44hd2ZiSFTBR|EhcW5iaIXtZY4hW0iVWUXZJINmdGy|IIXzbY5oKE2RQ2DBR{BieyC|dXLzeJJifGViYX\0[ZIhQCCqcoOgZpkhXUiSTFOtSXNKNU2VL13TJIFv[Wy7c3nzMEBKSzVyIE2gNE4xOTh6IN88UU4> M4rFfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkWwPVI2Lz5{N{[1NFkzPTxxYU6=
HEK293T M4rxcGZ2dmO2aX;uJIF{e2G7 M4j1[VMxKG2rboO= NHjuS5FKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFjERWMyKGW6cILld5Nm\CCrbjDISWszQTOWIHPlcIx{KHW|aX7nJGFkNUuJTFfLLGFkMS2PQ1GgZZMhe3Wkc4TyZZRmKGGodHXyJFMxKG2rboOgbY4heHKnc3XuZ4Uhd2ZiRGTUJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMEG5JO69VS5? M2fvdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUG5OlA1Lz5{OUWxPVYxPDxxYU6=
HEK293T M1PX[GZ2dmO2aX;uJIF{e2G7 NI\aWVA{OCCvaX7z MUPJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IH3veZNmKEiGQVO2JIV5eHKnc4Pl[EBqdiCKRVuyPVNVKGOnbHzzJINwNWW6cILld5NqdmdibX;1d4UhUESDMjD1d4lv\yCDYz3LS2xIUyiDYzmtUWNCKGG|IIP1ZpN1emG2ZTDh[pRmeiB|MDDtbY5{KGmwIIDy[ZNmdmOnIH;mJGRVXCCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCwMlAzQCEQvF2u NELDR4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxPVYxPCd-Mkm1NVk3ODR:L3G+
NFF MVTDfZRwfG:6aXPpeJkh[XO|YYm= Mk\ZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUmZHKGOnbHzzMEBKSzVyIE2gNE4zKM7:TT6= NFrMXXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK0OVQxOid-M{CyOFU1ODJ:L3G+
insect cell MnLaSpVv[3Srb36gZZN{[Xl? NUO0d3JbUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{[g[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR|UxKD1iMD6wNFg2KM7:TT6= NYTjUWFMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN|Y2QDl{PD;hQi=>
Sf21 NGrVNGpHfW6ldHnvckBie3OjeR?= NXfZd|MyUW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGZNSUdvSHnzMZRi\2enZDDmeYxtKGynbnf0bEBz\WOxbXLpcoFvfCCqdX3hckBJTEGFMTDlfJBz\XO|ZXSgbY4hW2Z{MTDpcpNm[3RiY3XscJMhfXOrbnegVmhMUyiDYznBUWMh[XNic4Xid5Rz[XSnIHL5JIZtfW:{ZYPj[Y5k\S2kYYPl[EBie3OjeTygTWM2OCB;IECuNFA6PCEQvF2u NFr1TFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO2OVg6Oid-M{CzOlU5QTJ:L3G+
insect cell NUnvVnR4TnWwY4Tpc44h[XO|YYm= M{WzTmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iSFTBR|MwVkORUkKg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR|UxKD1iMD6wNVI4KM7:TT6= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB|NkW4PVI9N2F-
insect cell NHjJNZNHfW6ldHnvckBie3OjeR?= NVrW[GtNUW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{Kg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR|UxKD1iMD6wNlY4KM7:TT6= M3zXOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{[1PFkzLz5|MEO2OVg6OjxxYU6=
insect cell MXjGeY5kfGmxbjDhd5NigQ>? NFLyUVZKdmirYnn0bY9vKG:oIFOteIVzdWmwYXygTIl{NXSjZ3fl[EBz\WOxbXLpcoFvfCCqdX3hckBJTEGFODDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemW|Y3XuZ4Uu[mG|ZXSgZZN{[XluIFnDOVAhRSByLk[wPUDPxE1w NUjqPIt[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN|Y2QDl{PD;hQi=>
A20 NUnvWoFrTnWwY4Tpc44h[XO|YYm= NGGxdpZDcW6maX7nJIFn\mmwaYT5JJRwf2G{ZIOgTGRCSzFiKFjpd5RwdmViZHXhZ4V1gWyjc3WgNUkhcW5ibX;1d4UhSTJyIHPlcIx{NCCneIDy[ZN{\WRiYYOgZolv\GmwZzDjc45{fGGwdDCodGtqMSxiS3mgQUAxNjByMUC3NkDPxE1w NXHMdYlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5OlA1QDlpPkGxPVYxPDh7PD;hQi=>
Hela NFT5SZJHfW6ldHnvckBie3OjeR?= MWHJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVt[SClZXzsd{BvfWOuZXHyJIV5fHKjY4TzJIJ6KG[udX;ybY1mfHKrYzDhd5NigSxiS3mgQUAxNjVyNDFOwG0v NIHwT|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWyNFU5OCd-MUm1NlA2QDB:L3G+
Sf9 NELkZXNHfW6ldHnvckBie3OjeR?= Mo\TPVAhdWmwcx?= MYXJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIF6teIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFNjDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiU3[5JIlve2WldDDj[YxteyC3c3nu[{BTUEuNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiOUCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlAxODV6IN88UU4> NELac4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
Sf21 M2\EdmZ2dmO2aX;uJIF{e2G7 MnfQOlAhdWmwcx?= MV;Jcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKENvdHXycYlv[WxiRlzBS{9JcXNvdHHn[4VlKEiGQVOxJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3cz3pcoZm[3SnZDDT[lIyKGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjByME[0JO69VS5? NF35Uow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell MoWySpVv[3Srb36gZZN{[Xl? M1XxelYxKG2rboO= NGHUeoZKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJJJm[2:vYnnuZY51KGi3bXHuJGMufGW{bXnuZYwhUGm|LYTh[4dm\CCKRFHDN{9PNXSncn3pcoFtKEeVVD30ZYdo\WRicnXjc41jcW6jboSgbJVu[W5iTlPPVlIhMDN7NTD0c{A1QDlicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdiUljLT{hC[ymDTVOgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMEC4OEDPxE1w M1flbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell MVnGeY5kfGmxbjDhd5NigQ>? NFTSbJI3OCCvaX7z MWLJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIFOteIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFMjDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KG[udX;ybY1mfGW{LDDLbUA:KDBwMECzOVUh|ryPLh?= NFfOR|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell NV7He4lrTnWwY4Tpc44h[XO|YYm= NV34cYtbUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDISGFEOTBiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCobIXvdo9o\W6rYzDw[ZB1cWSnIGLIT2tC[yCjczDzeYJ{fHKjdHWgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMES3OkDPxE1w M2TTNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell M3vRdmZ2dmO2aX;uJIF{e2G7 M1S0OVYxKG2rboO= NUfUZWNoUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDDMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{igLFEhfG9iM{e3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOtbY5n\WO2ZXSgbY5{\WO2IHPlcIx{KHW|aX7nJHJJUyiDYznLLGFkMUGPQzDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjF2NTFOwG0v NVfkVGpnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell M135R2Z2dmO2aX;uJIF{e2G7 MUTJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE5vdHXycYlv[WxiSHnzMZRi\2enZDDISGFEOTFiKEGgeI8hOzR5IILld4llfWW|KTDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemmvZYTldkwhU2liPTCwMlQxOSEQvF2u NFjkTpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
GM15850 MnXhSpVv[3Srb36gZZN{[Xl? MUGwMlEhfU1? NFLucIwyOiCqcoO= NW\sPWtiUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEePMUW4OVAh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5idH;0ZYwh[WOndInsZZRm\CCqaYP0c45mKGyndnXsJIF1KDBwMTD1UUBi\nSncjCxNkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
A549 NF\5SJhHfW6ldHnvckBie3OjeR?= M1W4T|AvOSC3TR?= NIDIPHNKdmirYnn0bY9vKG:oIGPJVnQzKGmwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMh[WKrbHn0fUB1dyCrbnT1Z4UhcHmyZYLhZ4V1gWyjdHnvckBw\iC2dXL1cIlvKGK7IGfld5Rmem5iYnzveEBidmGueYPpd{BifCByLkGgeW0> NEDpRXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G0PVg3OCd-MUexOFk5PjB:L3G+
ST14A NVzEXZVLTnWwY4Tpc44h[XO|YYm= NUnVNYt6PzVibl2= NWTmSGd[UW6mdXP0bY9vKG:oIGLFV3QwVlKVRj3jc5VxdGWmIILldJJme3Orb36gc4YhdHWlaX\ldoF{\SC2YXfn[YQhemG2IGLFNU9PWlOHIFLEUmYheHKxbX;0[ZIh[WO2aY\peJkh\XiycnXzd4VlKGmwIGPUNVRCKGOnbHzzJIF1KDd3IH7NJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHz1Z4ln\XKjc3WgZYN1cX[rdIm= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4NUm5N{c,OTd3NkW5PVM9N2F-
HEK293 NFjpd4tHfW6ldHnvckBie3OjeR?= NWniTmMxPjByIH7N MVPJcoNz\WG|ZTDpckBIWlB5ODDwdo91\WmwIHzleoVtKGmwIFjFT|I6OyClZXzsd{BifCB4MECgcm0h[nliaX3teY5w[myxdB?= NUjoO5NmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlY4OzJpPkG3OVY3PzN{PD;hQi=>
HEK293 MoDjSpVv[3Srb36gZZN{[Xl? NIj6eFQ3ODBibl2= M1zobmlv[3KnYYPlJIlvKHC{b4TlbY4h\Gm|dXzmbYRmKGm|b33ldoF{\SCuZY\lcEBqdiCKRVuyPVMh[2WubIOgZZQhPjByIH7NJIJ6KGmvbYXuc4Jtd3R? NF7LRpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2Olc{Oid-MUe1OlY4OzJ:L3G+
HeLa NUjuflU{TnWwY4Tpc44h[XO|YYm= MYexJJVO M2PmXlI1KGi{cx?= MX7JcoR2[3Srb36gc4YheDF4IHX4dJJme3Orb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6p Mmm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NEOxNVEoRjF5NkSzNVEyRC:jPh?=
HeLa Ml\kSpVv[3Srb36gZZN{[Xl? M3O3d|EhfU1? MUKyOEBpenN? Moe2TY5lfWO2aX;uJI9nKHB{NzDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91fGmwZx?= NVO5PGZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OFMyOTFpPkG3OlQ{OTFzPD;hQi=>
HeLa MmrxSpVv[3Srb36gZZN{[Xl? M3TNcFEhfU1? NYeybmJEOjRiaILz Mn\ZTY5lfWO2aX;uJI9nKHB{MTDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91fGmwZx?= NG\sRW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[0N|EyOSd-MUe2OFMyOTF:L3G+
HCT116 NUXlbXprTnWwY4Tpc44h[XO|YYm= Mkj1TY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDhd5Nme3OnZDDhd{BpcXO2b37lJIh6eGW{YXPleJlt[XSrb36gbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGK7IGfld5Rmem5iYnzveC=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB3NEKzPUc,OThyNUSyN|k9N2F-
HCT116 MmrZSpVv[3Srb36gZZN{[Xl? NVizSIdbUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOkBie3Onc4Pl[EBieyC2dXL1cIlvKGi7cHXyZYNmfHmuYYTpc44hcW5iaIXtZY4hUEOWMUG2JINmdGy|IHL5JHdme3Sncn6gZoxwfA>? M1HZZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEW0NlM6Lz5zOEC1OFI{QTxxYU6=
HEK293 STF M3vVcGZ2dmO2aX;uJIF{e2G7 MWGyOEBpenN? NVvveHlnSWO2aY\heIlwdiCxZjDtc5V{\SCZboSzZUB{cWewYXzpcoch\XiycnXzd4VlKGmwIFjFT|I6OyCVVF[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmVvYnHz[YQhdGGldHH0[UBl\Wi7ZILv[4Vv[XOnIHHzd4F6 MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB{Mk[3OUc,OTlyMkK2O|U9N2F-
NCI-H661 NHzIPYtHfW6ldHnvckBie3OjeR?= NUPpZ|d[OzByIH7N M3XG[|YhcHK| MUDJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gUmNKNUh4NkGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKGirc4TvcoUhUDRiYXPleJlt[XSrb36gZZQhOzByIH7NJIFnfGW{IE[gbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> M1fDRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{i1OlAxLz5zOUO4OVYxODxxYU6=
NCI-H661 NFq4[XVHfW6ldHnvckBie3OjeR?= NEHiXFU{ODBibl2= M13KXFYhcHK| NHHoXFVKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hVkOLLVi2OlEh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIHHsdIhiNXS3YoXsbY4h[WOndInsZZRqd25iYYSgN|AxKG6PIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NIfEU4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO4OVYxOCd-MUmzPFU3ODB:L3G+
HL60 MXvGeY5kfGmxbjDhd5NigQ>? MVeyNEB2\y:vbB?= M3rqelI1KGi{cx?= MkSzTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdiCjdDCyNEB2\y:vbDDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M2PlbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUO0OFcyLz5zOUWzOFQ4OTxxYU6=
primary bronchial epithelial cell MoT3SpVv[3Srb36gZZN{[Xl? M1;LOVAvOSC3TR?= M4iybGlvcGmkaYTpc44hd2ZiSFTBR|chcW5iaIXtZY4heHKrbXHyfUBjem:wY3jpZYwh\XCrdHjlcIlidCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gcZV1[W62IF\k[Yx1[TVyODDDSnRTKHC{b4TlbY4h[XCrY3HsJJN2em[jY3WgcI9k[WyrenH0bY9vKGG2IECuNUB2VQ>? NITrbG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
MCF7 MVPGeY5kfGmxbjDhd5NigQ>? NXu0WppPOC5zIIXN NHGx[pcyPiCqcoO= Ml;mTY5pcWKrdHnvckBw\iCVSWLUNkBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IIT1ZpVtcW5iaInw[ZJi[2W2eXzheIlwdiCjdDCwMlEhfU1iYX\0[ZIhOTZiaILzJIJ6KFenc4Tldo4h[myxdB?= NImwUpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MECzNFM1Oyd-MkCwN|A{PDN:L3G+
HeLa NH;T[HJHfW6ldHnvckBie3OjeR?= NVPlRpJsOSC3TR?= NGXrOmEyOCCvaX7z NFzxbW5KdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxJJVOKGGodHXyJFExKG2rboOgZpkh\my3b4LvcYV1emmlIHHzd4F6 Moi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyNUW4OFgoRjJzMEW1PFQ5RC:jPh?=
MCF7 M4jnSWZ2dmO2aX;uJIF{e2G7 MmHhNE42KHWP NIHPS28zPCCqcoO= M1rMeGlvcGmkaYTpc44hd2ZiQ3zhd5MhOSCqaYP0c45mKGSnYXPleJlt[XOnIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO|ZXSgZZMhfXBvcnXneYxifGmxbjDv[kBxOjFicILveIVqdiCuZY\lcEBifCByLkWgeW0h[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? MoPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN|Y{RC:jPh?=
MCF7 NYLoUpAyTnWwY4Tpc44h[XO|YYm= M364VlAvPSC3TR?= MXOyOEBpenN? NHX6U3lKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgZ4hidmenIHnuJJR2[nWuaX6gZYNmfHmuYYTpc44hdGW4ZXygZZQhOC53IIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
MCF7 MoHGSpVv[3Srb36gZZN{[Xl? M1jzRlAvPSC3TR?= NU\jVpNQOjRiaILz MnrHTY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IIXwMZJm\3WuYYTpc44hd2ZiYXPleJlt[XSnZDDobZN1d26nIFizJJBzd3SnaX6gcIV3\WxiYYSgNE42KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= Mm\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN|Y{RC:jPh?=
PC12 M1nReWZ2dmO2aX;uJIF{e2G7 NUTufnpTOTBidV2= NIDnVVI1QCCqcoO= NWjGcnlbUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDhZ4V1gWyjdHnvckBw\iCqaYP0c45mKEh|IHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{ MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
PC12 MWnGeY5kfGmxbjDhd5NigQ>? MorYNVAhfU1? MXi0PEBpenN? MXfJcohq[mm2aX;uJI9nKEiGQVO2JIlvKHKjdDDQR|EzKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:wIHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{ MoDPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
PC12 NF64d|JHfW6ldHnvckBie3OjeR?= NUnXeG9kOTBidV2= NUXafmVWPSCmYYnz NEnxdlNKdmS3Y4Tpc44hd2ZibnX1dol1\SCxdYTndo94fGhiaX6gdoF1KFCFMUKgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhPSCmYYnz MnnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
SH-SY5Y MkG3SpVv[3Srb36gZZN{[Xl? M3HsOVExKHWP MV:1JIRigXN? MkS2TY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{ M1X6NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
A549 M{O5UGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NXXLUIdnOC52IIXN M4m4dFI1KGi{cx?= NEjnbZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhe2i{dX7r[Y4h[W6mIIPoZZJxKHOqYYDlJIF1KDBwNDD1UUBi\nSncjCyOEBpenNiYomgdIhie2ViY3;ueJJie3RibXnjdo9{[2:yeR?= M3\LclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi0NVEzLz5{NUi4OFEyOjxxYU6=
HeLa NEX0bY9HfW6ldHnvckBie3OjeR?= NIjCRlFKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgeJVjfWyrbjDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IH;2[ZJvcWeqdDDifUBqdW23bn;mcJVwemW|Y3XuZ4UhdWmlcn;zZ49xcWNiYX7hcJl{cXN? NE\LXmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[xNVkyQSd-Mk[2NVE6OTl:L3G+
C127-LT M3G0emZ2dmO2aX;uJIF{e2G7 M3jvZVIxOCCwTR?= NUnpdppsOjRiaILz MYDJcohq[mm2aX;uJI9nKEiGQVOgbY4hdW:3c3WgR|EzPy2OVDDj[YxteyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKEWJRmCg[ZhxemW|c3nvckBifCB{MECgcm0h[W[2ZYKgNlQhcHK|IHL5JIZtfW:{ZYPj[Y5k\SCvaXPyc5Nkd3C7 M2TDWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm2N|czLz5{Nkm5OlM4OjxxYU6=
HeLa nuclear NEe5PXNHfW6ldHnvckBie3OjeR?= NYjz[JFjOTBidH:gNlUhfU1? M{fiUVMxKG2rboO= NX7qWY9vS2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBJTEGFIHnuJIh2dWGwIFjlUIEhdnWlbHXhdkBk\WyuczDheEAyOCC2bzCyOUB2VSC3c3nu[{BDd2NvYXPleJltNWy7c3nu[U1CVUNiYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiYomgUIlv\XenYY\ldk1DfXKtIIDsc5Qh[W6jbInzbZM> NGDTdlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa Ml\mSpVv[3Srb36gZZN{[Xl? MofONkB2VQ>? M4nFPFI1KGi{cx?= Mn2xTY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBJ\UyjIHPlcIx{KGG|c3Xzd4VlKGG|IHj5dIVz[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCB{IIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
HN13 MYnGeY5kfGmxbjDhd5NigQ>? MWWyOEBpenN? M336[GlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJVjF|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBpcXO2b37lJGg{KEy7c{mgZYNmfHmuYYTpc44hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJIF{e2G7 MnPSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{GwOlYoRjJ7MEOxNFY3RC:jPh?=
HeLaS3 M{HpTGZ2dmO2aX;uJIF{e2G7 NFu2OGIyOCC3TR?= MULJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN|IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTDt[YF{fXKnZDDpcY1m\GmjdHXsfUBjgSCrbX31co9jdG:2IHHuZYx6e2m| MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N|U9N2F-
HeLaS3 NFj0NZFHfW6ldHnvckBie3OjeR?= M3Pkc|ExKHWP NX7QW2tZOTZiaILz M{nLR2lvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjU{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm|dH;u[UBJO0t7IHHj[ZR6dGG2aX;uJIF1KDFyIIXNJJRz\WG2ZXSg[o9zKDF4IHjyd{Bnd2yub4fl[EBjgSClb33wc5Vv\CC5YYPoc5V1KGGwZDDt[YF{fXKnZDD1dEB1dyB{NDDodpMh[nliaX3teY5w[myxdDDhcoFtgXOrcx?= MlKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N|UoRjNyMkW4OVM2RC:jPh?=
Assay
Methods Test Index PMID
Western blot EGFR / STAT3 ; Cyclin D1 / CDK6 / CDK4 / p-RB / RB ; MMP-1 / MMP-3 / MMP-13 / TIMP-1 ; Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1) ; α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2 31289542 27431944 23802098
Immunofluorescence α-C/EBPβ / α-HP1α ; α-HDAC1 ; α-tubulin / acetylated histone 3 21122806 29045501 27957719
Growth inhibition assay Cell viability (A549 cells) ; Cell viability (MDA-MB-231 cells) ; Cell viability (PC3 cells) 27571418 27548148 31289542
In vivo Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro HDAC activity:

    Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

Cell Research:

[1]

  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
  • Concentrations: Dissolved in absolute ethanol, final concentrations ~10 μM
  • Incubation Time: 96 hours
  • Method:

    Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
  • Dosages: ~5 mg/kg/day
  • Administration: Injection s.c.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 23 mg/mL
(76.05 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

1.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 302.4
Formula

C17H22N2O3

CAS No. 58880-19-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT03901053 Recruiting Device: Reaktiv AFO|Device: PhatBrace AFO Foot Injuries and Disorders Jason Wilken|Minneapolis Veterans Affairs Medical Center|Walter Reed National Military Medical Center|Henry M. Jackson Foundation for the Advancement of Military Medicine|Center for Veterans Research and Education|University of Delaware|Johns Hopkins Bloomberg School of Public Health|University of Iowa February 27 2020 Not Applicable
NCT03887650 Recruiting Drug: Liposomal Bupivicaine 1.3%|Drug: Bupivacaine 0.5% Post-operative Pain|Total Shoulder Arthroplasty|Osteoarthritis of the Shoulder|Pain Management Hartford Hospital January 14 2019 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.

Frequently Asked Questions

Question 1:
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

Answer:
Our S1045 Trichostatin A (TSA) is R enantiomer.

Tags: buy Trichostatin A (TSA) | Trichostatin A (TSA) supplier | purchase Trichostatin A (TSA) | Trichostatin A (TSA) cost | Trichostatin A (TSA) manufacturer | order Trichostatin A (TSA) | Trichostatin A (TSA) distributor