ITSA-1 (ITSA1)

Catalog No.S8323

For research use only.

ITSA-1 (ITSA1) is an HDAC activator via TSA suppression, but shows no activity towards other HDAC inhibitors.

ITSA-1 (ITSA1) Chemical Structure

CAS No. 200626-61-5

Selleck's ITSA-1 (ITSA1) has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description ITSA-1 (ITSA1) is an HDAC activator via TSA suppression, but shows no activity towards other HDAC inhibitors.
Targets
HDAC [1]
(Cell-free assay)

Protocol (from reference)

Cell Research:[1]
  • Cell lines: A549 cells
  • Concentrations: 50 μM
  • Incubation Time: 17.5 h
  • Method: A549 cells ( 75% confluent) in 10-cm dishes are treated with DMSO (0.1%), TSA (500 nM), or tubacin (2 μM) for 4.5 h, and then either DMSO (0.1%) or ITSA1 (50 μM) is added for an additional 17.5 h. Samples are rinsed in PBS, trypsinized, and fixed for 2 h in 70% ethanol 30% PBS (4°C). Cells then are washed once in PBS and stored overnight in PBS (4°C).

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 292.12
Formula

C13H7Cl2N3O

CAS No. 200626-61-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C2C(=C1)N=NN2C(=O)C3=C(C=C(C=C3)Cl)Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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