TC-H 106

Catalog No.S6738 Synonyms: Pimelic Diphenylamide 106

For research use only.

TC-H 106 (Pimelic Diphenylamide 106) is a slow, tight-binding inhibitor of class I histone deacetylases(HDAC) with Ki value of 148 nM, about 102 nM, 14 nM for HDAC1, HDAC2, HDAC3, respectively.

TC-H 106 Chemical Structure

CAS No. 937039-45-7

Selleck's TC-H 106 has been cited by 1 Publication

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Biological Activity

Description TC-H 106 (Pimelic Diphenylamide 106) is a slow, tight-binding inhibitor of class I histone deacetylases(HDAC) with Ki value of 148 nM, about 102 nM, 14 nM for HDAC1, HDAC2, HDAC3, respectively.
Targets
HDAC3 [1]
(Cell-free assay)
HDAC2 [1]
(Cell-free assay)
HDAC1 [1]
(Cell-free assay)
14 nM(Ki) ~102 nM(Ki) 148 nM(Ki)
In vitro

The ability of TC-H 106 to prolong histone acetylation has also been observed in cell culture. Cells treated with TC-H 106 (at a sub-EC10 concentration as determined with an MTS cell proliferation assay) have hyperacetylated histone H3. These cells do not lose significant amounts of acetylated histone H3 even after removal of the inhibitor for hours.[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: lymphoblastoid cell line
  • Concentrations: 2 μM
  • Incubation Time: 24 h
  • Method:

    Cells are cultured in RPMI 1640 media with 10% fetal bovine serum and 10 mM HEPES, at 37 °C in 5% CO2. After the 5th split, the cells are treated with inhibitor TC-H 106 (2 μM) for 24 h, in culture medium. 24 h after treatment, the cells are washed twice with Hanks' balanced salts buffer to remove the inhibitor. A portion of the cell population is then harvested immediately after washing, and referred to as a time 0 point (24 h treatment point), and the rest of the cells are then re-cultured in cell culture media without added inhibitor. The re-cultured cells and controls are then harvested every hour for a total of 7 h.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 339.43
Formula

C20H25N3O2

 

CAS No. 937039-45-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CC=C(C=C1)NC(=O)CCCCCC(=O)NC2=CC=CC=C2N

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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