3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

For research use only.

3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.

3-Methyladenine (3-MA) Chemical Structure

CAS No. 5142-23-4

Selleck's 3-Methyladenine (3-MA) has been cited by 649 publications

Purity & Quality Control

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Biological Activity

Description 3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 MWXGeY5kfGmxbjDBd5NigQ>? Mki0NVBuVQ>? M1XBT|Fp M3z2UoRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= Mk\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NkSwN|coRjJzOE[0NFM4RC:jPh?=
Jurkat MVvGeY5kfGmxbjDBd5NigQ>? NXruPIgxOTCvTR?= MYKxbC=> MWjk[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gUGM{NUmLIHHu[EB1cGViZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MoTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NkSwN|coRjJzOE[0NFM4RC:jPh?=
HeLa  NUDrfmRHS3m2b4TvfIlkcXS7IFHzd4F6 MkXjNo1O NIq4UJkzPGh? NWXIO|N2cW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv MnnFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{WzPFUoRjJzOEe1N|g2RC:jPh?=
PC12/TetOn Ml;6SpVv[3Srb36gRZN{[Xl? M37oOFAvOS9zbV2= NFLPVYgyQGh? NXzNT41FdGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= NH\yfog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwOlY2QSd-MkG5NFY3PTl:L3G+
RMPI8226  NIjDd2hHfW6ldHnvckBCe3OjeR?= NYi2OINmPW2P M{Wyc|Fp NG\v[mF{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w M3GxSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUG1OlIxLz5{MUmxOVYzODxxYU6=
MCF-7 MVXGeY5kfGmxbjDBd5NigQ>? MUexNI1O MV60PIg> NHPNe4RjdG:la4OgZZV1d3CqYXf5JIlv\HWlZXSgZpkh[m:{dHX6c41q[g>? M2L5WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUOxPVM4Lz5{MUmzNVk{PzxxYU6=
HBx  NIrTNpZCeG:ydH;zbZMhSXO|YYm= MVWxNI1O MV60PIg> M1WzWmROW09? NXGxRpdEcW6lcnXhd4V{KGOnbHyg[IVifGh? M1TEdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKwNFc5Lz5{MkCyNFA4QDxxYU6=
Marc-145 MlO2SpVv[3Srb36gRZN{[Xl? NV3q[YNIPW2P MnHWNVIwOjRxM{\o NYTXXWtJemWmdXPld{B1cGViUGLSV3YhfGm2ZYLzJIFv\CC2aHWgdJJwfGWrbjDlfJBz\XO|aX;u MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzOUmwNEc,OjJzMUm5NFA9N2F-
U937 MVjGeY5kfGmxbjDBd5NigQ>? MmLKNo1O MnX4NVJp MW\k[YNz\WG|ZYOgeIhmKGG3dH;wbIFogSC{YYTpc:Kh MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF3NUG1NEc,OjJzNUWxOVA9N2F-
BGC-823 NXnEe2RITnWwY4Tpc44hSXO|YYm= MUC1cW0> NHrPWoYzcA>? NX\hWHdtcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? NGf1dXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNlE2Oid-MkKzNlIyPTJ:L3G+
A549 NF\ke2dHfW6ldHnvckBCe3OjeR?= NGHj[2IxNjGvTR?= NYC0N49VOjSq MXHzeZBxemW|c3XzJHNWOTF{N{StbY5lfWOnZDDj[YxtKGSnYYTo NEixc3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS2Olk3OCd-MkK0OlY6PjB:L3G+
pDCs MYnGeY5kfGmxbjDBd5NigQ>? Ml\DNVBuVQ>? NV;ZeWJOOC53aB?= M{i5UeKhemWmdXPld{B1cGViaX7keYN1cW:wIH;mJGlHVi4QsTDifUB{e1KQQUSw NGHZZ|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO5OlU6QSd-MkKzPVY2QTl:L3G+
HeLa  MVPBdI9xfG:|aYOgRZN{[Xl? MX61cW0> M2nJdFI1cA>? NHL4PY1qdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> M4HYW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUS1NVI5Lz5{MkW0OVEzQDxxYU6=
U251  MXjBdI9xfG:|aYOgRZN{[Xl? M1rmUVVuVQ>? NELDfY0zPGh? NFHW[3lqdmO{ZXHz[ZMhWzFvaX7keYNm\CClZXzsJIRm[XSq MnnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3N{m3PFgoRjJ{NUe5O|g5RC:jPh?=
MCF-7  MVjBdI9xfG:|aYOgRZN{[Xl? MYmwMlFuVQ>? M4e4U|Zp MVTlcohidmOnczDzbZJ1cW6xbD3pcoR2[2WmIHHwc5B1d3Orcx?= MnTxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NUG5PFkoRjJ{N{WxPVg6RC:jPh?=
PC-3  MXjBdI9xfG:|aYOgRZN{[Xl? M3fHcVJuVQ>? MXuybC=> Mn;kbY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? M{D6XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S1OVgxLz5{Mke0OVU5ODxxYU6=
HCT116 MUTBdI9xfG:|aYOgRZN{[Xl? M4D3d|VuVQ>? NWXXXnVTOjSq NHLY[WtFVVOR MULlcohidmOnczDhdIlo\W6rbj3pcoR2[2WmIHPlcIwh\GWjdHi= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkWyNkc,OjR4Mk[1NlI9N2F-
U2OS NGX3O|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVGxNI1O MofmNlRp MWfpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDEc5g> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OUCxN{c,OjR4M{mwNVM9N2F-
A2780cp NXPERVFFSXCxcITvd4l{KEG|c3H5 M2TMXFIvPW2P Ml3ENYg> NYPETZU5\GSKMl:= NUPtTZRY\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NFjTcpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixO|k1Pid-MkS4NVc6PDZ:L3G+
HepG2 NEPQTVZHfW6ldHnvckBCe3OjeR?= MmTCOY1O M1P6SlRp NVzYZ4RocW6lcnXhd4V{KGOnbHz1cIFzKGyndnXsd{Bw\iCKTNMg M1TyTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{GzOVg4Lz5{NEexN|U5PzxxYU6=
Microglia  MWfBdI9xfG:|aYOgRZN{[Xl? MUe1cW0> M2O0[VI1cA>? MWnk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzOE[wNUc,OjR6MUi2NFE9N2F-
MDA-MB 231 NU[zToVmSXCxcITvd4l{KEG|c3H5 M3W3SVVuVQ>? NYnZZnF3OC53aB?= NXrHUVdGdW:mdXzheIV{KFSxY3;tbY7DtsLiaX7keYNm\CCjcH;weI9{cXN? NVXGfVRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4N|A4QDFpPkK0PFMxPzhzPD;hQi=>
PANC-1  MmCxRZBweHSxc3nzJGF{e2G7 NXqwR|NPOW2P Mn;tOFhp M1nsPWROW09? NHG1OmdmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NFLneG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0NlE2QCd-MkS4OFIyPTh:L3G+
MDA-MB-231 NIn6bGZHfW6ldHnvckBCe3OjeR?= MkfSNo1O Ml7aOFhp MlzndJJwdW:2ZYOgWG0ucW6mdXPl[EBk\WyuIHTlZZRp M3XwTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
MDA-MB-231 NUTHcmZFTnWwY4Tpc44hSXO|YYm= MXyycW0> MlLENlRp MXvpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NUjLb4t3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
MCF-7  NVTTcXpQTnWwY4Tpc44hSXO|YYm= MXmycW0> M4fDXFQ5cA>? NITI[Hpxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl5ME[3Okc,OjR7N{C2O|Y9N2F-
MCF-7  MnTHSpVv[3Srb36gRZN{[Xl? Ml3FNo1O NHLRTG0zPGh? M2\kdIlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= M{TWUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
HepG2 MVrBdI9xfG:|aYOgRZN{[Xl? NXrXfodpO22P MVW1bC=> NG\2TWlz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgVWR{ NHPaRWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizOlU6PSd-MkK4N|Y2QTV:L3G+
HeLa NFna[5lCeG:ydH;zbZMhSXO|YYm= NWe4SpIyOTCvTR?= M1H6b|Jp Mmqz[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2bXXueEB4cXSqIGDFTS=> M3fP[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MECwNVM2Lz5{M{CwNFE{PTxxYU6=
SK-HEP-1 MXjBdI9xfG:|aYOgRZN{[Xl? M4DzOVExdU1? M33UdVFp NIfYPVhxem:2ZXP0d{Bi\2GrboP0JIF2fG:yaHHnfUBidmRiaX7keYNmeyCjcH;weI9{cXNiaX6gZpVn[Wyrbj30doVifGWmIHPlcIx{ MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh3OE[0PUc,OjJ6NUi2OFk9N2F-
MDA-MB231 NVvlTZBSTnWwY4Tpc44hSXO|YYm= NEfBN3c2dU1? NX3N[HcxOWh? MWjpcoNz\WG|ZYOgdoV{fmW{YYTyc4wudWWmaXH0[YQh[2G|cHHz[UBi[3SrdnH0bY9vKGGwZDDj[YxtKGSnYYTo M4W4bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEi4PFUxLz5{M{C4PFg2ODxxYU6=
PaCa44 MYPBdI9xfG:|aYOgRZN{[Xl? NYjtO2tPOi53bV2= MYixbC=> M1\EU5Jm\HWlZYRCpIdmdmmyaX6tcYVlcWG2ZXSgZZBweHSxc3nz MoS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzMkSxNVIoRjJ|MUK0NVEzRC:jPh?=
T-47D M3vBVWZ2dmO2aX;uJGF{e2G7 M1TDUlExdU1? NY[4XnpEOmh? MWDpcohq[mm2czDheZRweGijZ4mgdJJw[2W|czDhcoQhcW6lcnXhd4V{KHKjcHHtfYNqdiCrbnT1Z4VlKGGyb4D0c5Nqew>? NFT1TlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OwNFAzPid-MkOzNFAxOjZ:L3G+
GTL-16  NFTFb41CeG:ydH;zbZMhSXO|YYm= NFzyZYs2dU1? M13NSVI1cA>? MkPRdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= M13HbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{GzOFkxLz5{M{OxN|Q6ODxxYU6=
U251MG  MWjGeY5kfGmxbjDBd5NigQ>? NEXSO3k{dU1? NWTTdlVZOWh? MmXvd5VxeHKnc4Pld{BNSzNvSVmgdJJwfGWrbjDlfJBz\XO|aX;u NWL4ZY1iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzN|g3OThpPkKzN|M5PjF6PD;hQi=>
T24 MV;GeY5kfGmxbjDBd5NigQ>? Ml7iNVBuVQ>? M3m0OVFp Mo\pdoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= NF3QfWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O1OFA5OCd-MkOzOVQxQDB:L3G+
HUVECs NV7NNmtiTnWwY4Tpc44hSXO|YYm= MmThN41O MnrXNlRp MYHicI9kc3NidHjlJJBzd3SnY4TpeoUh\W[oZXP0JI9nKHKnc4\ldoF1em:uIHL5JIlvcGmkaYTpcoch[XW2b4DoZYd6 NWjWbIVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOVg6OjhpPkKzN|U5QTJ6PD;hQi=>
MCF-7  MkLISpVv[3Srb36gRZN{[Xl? MVW1cW0> NHixTGEzPGh? NWXHd3dWcW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NU[3PUc,OjN|OUW2O|k9N2F-
Hela  NEfDRlhHfW6ldHnvckBCe3OjeR?= NYfqTIExPW2P MoXpNlRp MWTpcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 M2nkU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{m1Olc6Lz5{M{O5OVY4QTxxYU6=
OR6 MlG3SpVv[3Srb36gRZN{[Xl? M3r0c|ExdU1? NV:wZlhWPzKq MX\zeZBxemW|c3XzJGhEXiC{ZYDsbYNifGmxbjDhcoQh\m:{bXH0bY9vKG:oIHH1eI9xcGGpb4PvcYV{ NU\RPXc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPVU5PzVpPkKzN|k2QDd3PD;hQi=>
HT-29  MVzGeY5kfGmxbjDBd5NigQ>? NWTBdFBsOW2P NH3VS4Q1QC97Nni= NVPaN5lucW6qaXLpeJMhSU2SSzDpcoR2[2W|IHH1eI9xcGGpaXOgZ4VtdCCmZXH0bC=> M3v4WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUC4NlczLz5{M{WwPFI4OjxxYU6=
SH-SY5Y MlTPR5l1d3SxeHnjbZR6KEG|c3H5 NGTzRpM2dU1? NHLMUIczPGh? Mn;sbY5kemWjc3XzJHBEViC2b4jpZ4l1gQ>? MonrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkWyOlUoRjJ|NUK1NlY2RC:jPh?=
Saos-2  NUTTdopYSXCxcITvd4l{KEG|c3H5 NIradWwydU1? NF;6W|g6Pmh? M1i4dolv[3KnYYPld{Bk\WyuIHTlZZRpKGmwZIXj[YQh[nliUFPY MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV4M{G3NUc,OjN3NkOxO|E9N2F-
1321N1 NGjDc3hEgXSxdH;4bYNqfHliQYPzZZk> NITZVYo2dU1? M3;0XlI1cA>? M2nmc5Bzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= MlrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkWyOlUoRjJ|NUK1NlY2RC:jPh?=
A2780 M33kWWFxd3C2b4Ppd{BCe3OjeR?= NEW3[5M2dU1? M2XWfFI1cA>? M3TH[4Nwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| M3KwUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUmyNlgyLz5{M{W5NlI5OTxxYU6=
OV2008 MnfoRZBweHSxc3nzJGF{e2G7 NEHUc3g2dU1? NHrw[ZEzPGh? MV7jc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? NIHRbIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5NlI5OSd-MkO1PVIzQDF:L3G+
PC12  M1LkOWZ2dmO2aX;uJGF{e2G7 Mmr5NVBuVQ>? MnPtNlRp NIOxclR4[XSnch?= MoXDxsBqdmirYnn0d{BkcHmvb4TyfZB{cW5vbHnr[UBxem:2ZXHzc41idCCjY4Tpeol1gS5? Mo\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEO5O|koRjJ|NkCzPVc6RC:jPh?=
SH-SY5Y NEXJZYlCeG:ydH;zbZMhSXO|YYm= M3;leFVuVQ>? M2nreVFp NIO2Umpi[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S= NHy4WIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xPVM6PSd-MkO2NVk{QTV:L3G+
SH-SY5Y NVPBWXA6TnWwY4Tpc44hSXO|YYm= MorsNY1O MWWyOIg> NXnQZ|J6cW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JHRQS1B? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2M{G0PEc,OjN5NEOxOFg9N2F-
HepG2 M3\0TWZ2dmO2aX;uJGF{e2G7 MnzwNVBuVQ>? NH\XfGczPGh? M4nBdYlvcGmkaYTzJJNqXEmJQWKtJIFv\CCKQmPTMYlv\HWlZXSgZZV1d3CqYXf5 Moi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MUewOFAoRjJ|OEG3NFQxRC:jPh?=
HeLa NEfxS41HfW6ldHnvckBCe3OjeR?= MXOxNI1O M3zJW|Jp NHjTbWd{fXCycnXzd4V{KEyFMzDJTUBmgHC{ZYPzbZNwdg>? M4rxVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OE[0O|M5Lz5{M{i2OFc{QDxxYU6=
HONE-1  MmfGSpVv[3Srb36gRZN{[Xl? NVjESW9NPW2P NY\zS4dsOWh? M3X2O5JmeHKnc4Pld{A3ei2vZXTpZZRm\CCUT2OgdJJw\HWldHnvci=> M1nXZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEmyN|U5Lz5{M{i5NlM2QDxxYU6=
MCF7 M1juOmZ2dmO2aX;uJGF{e2G7 MlvWOY1O NXjIZ21XOjSq NV33[2hzcW6lcnXhd4V{KEO3TzDpcoR2[2WmIHPlcIwh\GWjdHi= MkP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkK2NlkoRjJ|OU[yOlI6RC:jPh?=
HO8910 NFfiepdCeG:ydH;zbZMhSXO|YYm= MknsNVBuVQ>? MlzZNVJp Mn;I[Y5p[W6lZYOgRlE6NWmwZIXj[YQh[XCxcITvd4k> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl6M{[xNEc,OjN7OEO2NVA9N2F-
SMMC-7721 M{DmWmFxd3C2b4Ppd{BCe3OjeR?= MXi1cW0> NVHDcms{OjSq NWfQVGRK[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ NVHHbYxRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlY3QTNpPkK0NFY3Pjl|PD;hQi=>
Hep3B NILxWXZCeG:ydH;zbZMhSXO|YYm= NYezSopyPW2P MlLsNlRp MkO5ZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NFfoeFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2OlY6Oyd-MkSwOlY3QTN:L3G+
H460 NWf1fHJtTnWwY4Tpc44hSXO|YYm= MWSxNI1O MWO0bC=> M{Xzc4lv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NYXndFVzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|MzODhpPkK0NVc{OjB6PD;hQi=>
A549 NUTqVHA3TnWwY4Tpc44hSXO|YYm= NX\SbpdQOTCvTR?= Ml31OIg> NW\5d2tKcW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliaYLyZYRq[XSrb36= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2MkezOUc,OjRzNEK3N|U9N2F-
H1299  NIXQUW5HfW6ldHnvckBCe3OjeR?= MW[xNI1O NGLSVoQ1cA>? M17KVYlv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NFLjb5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3N|IxQCd-MkSxO|MzODh:L3G+
WiDr NHjkZ4ZHfW6ldHnvckBCe3OjeR?= M4\tVlExdU1? MVGxbC=> MXLpcohq[mm2czDQR2JNNWmwZIXj[YQhVEN|IFnJJIV5eHKnc4Ppc44> MlTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOUC0PFkoRjJ2MUmwOFg6RC:jPh?=
LoVo  NVPtS4U6SXCxcITvd4l{KEG|c3H5 NEjjXIw2dU1? MWC0PIg> M2nDUYVvcGGwY3XzJGRESS2rbnT1Z4VlKGGyb4D0c5Nqey5? NWr5V3pNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFE5OTJpPkK0NlAyQDF{PD;hQi=>
HepG2 E47 MWXGeY5kfGmxbjDBd5NigQ>? NHzEZmUzNjWvTR?= MkXDOFhp NHH6VYtqdmO{ZXHz[ZMhfGinIITvfIlkcXS7IH;mJGFCNCCEU1:sJIFv\CCFQ3y0 NIDBd5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK3N|c{QCd-MkSyO|M4Ozh:L3G+
RKO NX7L[XY4TnWwY4Tpc44hSXO|YYm= MojsNo1O MWqxbC=> NXPZWYttTE2VTx?= M2ridIVvcGGwY3XzJINmdGxiZHXheIgh[nliZ3Xs[IFv[W27Y3nu NIfpVnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5NVc4Pyd-MkSyPVE4Pzd:L3G+
Hep3B NHTHOodCeG:ydH;zbZMhSXO|YYm= M3PU[VJuVQ>? M2DKPFEzcA>? MYXEUXNQ NVTCZphocW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> NFjzblY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5O|MxOCd-MkSyPVc{ODB:L3G+
ACHN-5968 MkDzRZBweHSxc3nzJGF{e2G7 MVW1cW0> MXqzbC=> MVvlcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh NFTLN4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwOVYxPCd-MkSzNFU3ODR:L3G+
Huh7 M1GwemFxd3C2b4Ppd{BCe3OjeR?= NX;iT2FsOm2P M3PKS|EzcA>? NX7EZVgyTE2VTx?= M1PXe4lvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo NHjtO5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5O|MxOCd-MkSyPVc{ODB:L3G+
UOK257 NXHvNWNCSXCxcITvd4l{KEG|c3H5 M1fRPFVuVQ>? MYSzbC=> NWXwWmJt\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= NIHuSXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwOVYxPCd-MkSzNFU3ODR:L3G+
ECSCs  NVP4cIViSXCxcITvd4l{KEG|c3H5 MkHkNVBuVQ>? M4nFTlRp Mlq2[IVkemWjc3XzJJJieGGveXPpck11emWjdHXkJIFxd3C2b4Ppdy=> MmrrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUmxNFkoRjJ2M{G5NVA6RC:jPh?=
MCF-7  NVHBN21ZTnWwY4Tpc44hSXO|YYm= NVfKWmxIOTCvTR?= NGf2S2MzPGh? MljtbY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IHPo[Y1wfGincnHwfUBlenWpcx?= M1z0fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G1OVc5Lz5{NEOxOVU4QDxxYU6=
SGC-7901  NGi0Z|ZCeG:ydH;zbZMhSXO|YYm= M2nrfFJuVQ>? NHP6NJoycA>? M3PSO4lv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| NFrWOWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyNVM1OCd-MkSzNlE{PDB:L3G+
SMMC-7721 M2nme2Fxd3C2b4Ppd{BCe3OjeR?= NVjFUpZ1Om2P MV6xbC=> M{\2TYlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| M4\FWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{KxN|QxLz5{NEOyNVM1ODxxYU6=
T24 MoLyRZBweHSxc3nzJGF{e2G7 MY[1cW0> M{HaRlEvPWh? MWHwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> M3zCWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{S5NVc3Lz5{NEO0PVE4PjxxYU6=
NTUB1 MmDqRZBweHSxc3nzJGF{e2G7 NFnI[ZA2dU1? MY[xMlVp NW\zPJJ7eG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| M{Cz[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{S5NVc3Lz5{NEO0PVE4PjxxYU6=
MG-63 NFnqW4tCeG:ydH;zbZMhSXO|YYm= Ml7kNVBuVQ>? NFzwbpIyOmh? NX;CbZdQ\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? M4P1OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|AyLz5{NEO1PFMxOTxxYU6=
MG-63 MYHBdI9xfG:|aYOgRZN{[Xl? M4XwSlAvPS9zbV2= NHLqUms{Omh? MlPH[Y5p[W6lZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OEO0Nkc,OjR|NUizOFI9N2F-
MG-63 M3n6dGZ2dmO2aX;uJGF{e2G7 MYqwMlUwOW2P NV;IfIZGPDiq NXv3dGp[cW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? NGfM[ZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
U2OS  MXXGeY5kfGmxbjDBd5NigQ>? NFnjRmMxNjVxMX3N MWW0PIg> NXKyd5JjcW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? M2TqT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|QzLz5{NEO1PFM1OjxxYU6=
HGC-27 NE\zWpJHfW6ldHnvckBCe3OjeR?= NHLMdpMyOG2P M{j0blFp MkjRbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JIxwe3NiYomgVmFFODBzIH;yJG1MNTJ{ME[= MoHFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MU[zOFkoRjJ2NEG2N|Q6RC:jPh?=
HCT116  NFvxZ3RCeG:ydH;zbZMhSXO|YYm= M1z5ZVVuVQ>? NWjK[4J{OjSq NH:zSoNmdmijbnPld{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgZZBq\2WwaX6= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkWyNkc,OjR4Mk[1NlI9N2F-
A549 NIKxbWFCeG:ydH;zbZMhSXO|YYm= MVOxNI1O MUO0PIg> M4XINIFk[2WuZYLheIV{KHSqZTDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBRXFh? NGXWfoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[yOlczOid-MkS2NlY4OjJ:L3G+
Saos-2 NX7iNVk1SXCxcITvd4l{KEG|c3H5 M3L4SVExdU1? M4DiZ|I1cA>? M3yzSolvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? M1S4clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkO5NFE{Lz5{NE[zPVAyOzxxYU6=
U2OS NV:y[21USXCxcITvd4l{KEG|c3H5 M4jjS|ExdU1? NH\mZmYzPGh? MV7pcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MlfLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{mwNVMoRjJ2NkO5NFE{RC:jPh?=
HepG2 NYT3e243TnWwY4Tpc44hSXO|YYm= NEexUGo2dU1? NV;uR4VTPGh? NEfoUI9qdmO{ZXHz[ZMhUExicnXs[YF{\Q>? M{jIUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{GzOVg4Lz5{NEexN|U5PzxxYU6=
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BT-37 MojZdWhVWyCjc4PhfS=> MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN? NU\s[WpMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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MG 63 (6-TG R) MVrxTHRUKGG|c3H5 NV[3TldmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz NXq1N2JSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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LAN-5 MYXxTHRUKGG|c3H5 MUfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NV;mNXhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Assay
Methods Test Index PMID
Western blot α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 ; caspase-3 / caspase-9 / PARP ; VEGF ; APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2 29296191 25891159 26316755
Immunofluorescence LC3 / Hif-α / COX2 29039446
Growth inhibition assay Cell viability 26934124
In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol (from reference)

Kinase Assay:[4]
  • Protein degradation assay:

    HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.

Cell Research:[2]
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
  • (Only for Reference)
Animal Research:[5]
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 10 mg/mL warmed
(67.04 mM)
Ethanol 4 mg/mL
(26.81 mM)
DMSO 3 mg/mL warmed
(20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage 3 years -20°C powder
Smiles CN1C=NC(=N)C2=C1N=CN2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

Answer:
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

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