3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D NYXYR4NZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXiT4s{O22P M3u0cFJp NVvES|BrTE2VTx?= MoLidoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? Ml7TNlU4OTZ3NkG=
A549  NVjCfIQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHBSWM{dU1? M2eyXFJp NHvaVJdFVVOR MlzydoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? NV\JbGRtOjV5MU[1OlE>
95D NUT4PHd[SXCxcITvd4l{KEG|c3H5 MUW1cW0> M1jvOVJp NWnaOlZUTE2VTx?= NFPKfItqdmirYnn0d{BDTE2FLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHi= M3zRc|I2PzF4NU[x
A549  NEXxe3hCeG:ydH;zbZMhSXO|YYm= NWmyO|JpPW2P NVe2OVluOmh? NUHSSlBiTE2VTx?= NV7xOlBjcW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo NWnqRYVKOjV5MU[1OlE>
NBL-W-S  MmnzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3u1fFFuVQ>? MWC2bC=> NV36T4JH\W6qYX7j[ZMhT0GQVD22NUB1d3irY3n0fS=> M1PvUVI2OzJ|MkKy
NBL-W-S  NGnRb4hCeG:ydH;zbZMhSXO|YYm= NEXHXWcydU1? MVi2bC=> MUPpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDHRW5VNTZz NUfwfWtmOjV|MkOyNlI>
A2780cp  NH\rXGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PFRlFuVQ>? NGTuTY8ycA>? M13HZ4lv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NWXCSoNROjV|MkK2PVQ>
A549  MnG1SpVv[3Srb36gRZN{[Xl? NELBRZY2dU1? MmDlNog> NFHLeIJ{fXCycnXzd4V{KFOSQzDpcoR2[2WmIHHjZ5VufWyjdHnvckBw\iCOQ{OtTWk> NGe0bGEzPTJ6NU[yPC=>
H157 MXPGeY5kfGmxbjDBd5NigQ>? MXm1cW0> NGfzSYszcA>? NH3VOo1{fXCycnXzd4V{KFOSQzDpcoR2[2WmIHHjZ5VufWyjdHnvckBw\iCOQ{OtTWk> MUiyOVI5PTZ{OB?=
MDR NUf2emxqSXCxcITvd4l{KEG|c3H5 MX:xNI1O NYDQOXFLPmh? NYTxOoRIe3S{ZX7neIhmdnNidHjlJJBwf2W{IH;mJIFvfGmlYX7j[ZIh\HK3Z4O= NHqxSlczPTBzOUewNS=>
HT-29  NX3DVJFYSXCxcITvd4l{KEG|c3H5 NH7se4cydU1? MUO0PIg> MWrEUXNQ NEnJRmNmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NWPqRppvOjR6NEKxOVg>
Microglia  MmC0RZBweHSxc3nzJGF{e2G7 MWC1cW0> NFTmZpEzPGh? NEH4dWpl\WO{ZXHz[ZMhcHmyb4jpZU1qdmS3Y3XkJINmdGxiZHXheIg> NHH3NVMzPDhzOE[wNS=>
A2780cp MU\BdI9xfG:|aYOgRZN{[Xl? NW\McGpPOi53bV2= NH6yR40ycA>? Ml7a[IRJOk9? M2rHVoVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NH7Ze5gzPDhzN{m0Oi=>
HepG2 MmLzSpVv[3Srb36gRZN{[Xl? MYi1cW0> NIfpNWY1cA>? Mor3bY5kemWjc3XzJGhNKHKnbHXhd4U> MkH0NlQ4OTN3OEe=
A549 MX;BdI9xfG:|aYOgRZN{[Xl? MWK1cW0> MUi0PIg> MUfk[YNz\WG|ZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdCCmZXH0bEBidmRiZYjwdoV{e2mxbjDs[ZZmdHNib3[gZ4F{eGG|ZT2zMEBD\WOuaX6tNUBidmRiTFOzMWlK NIDM[Y0zPDdyNkOwNy=>
U2OS Mk[2RZBweHSxc3nzJGF{e2G7 MnnINVBuVQ>? M4nOcFI1cA>? Mof2bY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJJRp\SCXMl;TJINmdGy|IHnu[JVk\WRiYomgSI95 M2P3bFI1PjN7MEGz
Saos-2 NIXrWlJCeG:ydH;zbZMhSXO|YYm= Ml3MNVBuVQ>? MkXuNlRp NYj6dpdjcW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 NFrLbHczPDZ|OUCxNy=>
A549 NXXlWHdXSXCxcITvd4l{KEG|c3H5 MlnWNVBuVQ>? M3fQXVQ5cA>? NEXr[Whi[2OnbHXyZZRmeyC2aHWgdoVlfWO2aX;uJI9nKGOnbHygeoli[mmuaYT5JIlv\HWlZXSgZpkhWFS[ NXziNIx5OjR4Mk[3NlI>
HCT116  M4LnPGFxd3C2b4Ppd{BCe3OjeR?= MorVOY1O MV6yOIg> MX\lcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? NXHhbmZSOjR4Mk[1NlI>
HGC-27 MVXGeY5kfGmxbjDBd5NigQ>? MVixNI1O M2f2T|Fp MWTpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgcI9{eyCkeTDSRWQxODFib4KgUWsuOjJyNh?= MmSzNlQ1OTZ|NEm=
U2OS  M3rtUGZ2dmO2aX;uJGF{e2G7 MmH4NE42NzGvTR?= MX:0PIg> MVTpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> MXSyOFM2QDN2Mh?=
MG-63 MYXGeY5kfGmxbjDBd5NigQ>? M3rJUlAvPS9zbV2= NFvrdmU1QGh? MnHWbY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> NXjoc5JJOjR|NUizOFI>
MG-63 MX;BdI9xfG:|aYOgRZN{[Xl? NXe4fHR5OC53L{HtUS=> NUCxPIFEOzKq MVjlcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| MnTxNlQ{PTh|NEK=
MG-63 MVTBdI9xfG:|aYOgRZN{[Xl? NX\mNpJEOTCvTR?= NUTETpRyOTKq NEj1N5FmdmijbnPld{BFWC2rbnT1Z4VlKGGyb4D0c5Nqew>? MVOyOFM2QDNyMR?=
NTUB1 NFzLXXJCeG:ydH;zbZMhSXO|YYm= M1XUTFVuVQ>? NGDUXIEyNjWq MYPwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> NXLoXJpNOjR|NEmxO|Y>
T24 MV\BdI9xfG:|aYOgRZN{[Xl? MnrTOY1O NIC2c4UyNjWq NHHocnBxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? MYSyOFM1QTF5Nh?=
SGC-7901  NUOyU5B2SXCxcITvd4l{KEG|c3H5 NHPSb5QzdU1? MkjnNYg> M4mzXolv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| M2frZVI1OzJzM{Sw
SMMC-7721 M1jFVGFxd3C2b4Ppd{BCe3OjeR?= NG\6V5MzdU1? M1TnSFFp MWHpcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? M1vMU|I1OzJzM{Sw
ECSCs  Mn;ERZBweHSxc3nzJGF{e2G7 NGe1UXkyOG2P M3HMclRp MnHq[IVkemWjc3XzJJJieGGveXPpck11emWjdHXkJIFxd3C2b4Ppdy=> MWiyOFMyQTFyOR?=
MCF-7  MmfISpVv[3Srb36gRZN{[Xl? Mn3UNVBuVQ>? NXzOdIt6OjSq NIDZUGZqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgZ4hmdW:2aHXyZZB6KGS{dXfz NGjBR3kzPDNzNUW3PC=>
UOK257 MmDKRZBweHSxc3nzJGF{e2G7 NVjJTFRtPW2P NILiRpY{cA>? NUnLcldY\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= NGi2Z5MzPDNyNU[wOC=>
ACHN-5968 NH\6ZnBCeG:ydH;zbZMhSXO|YYm= MXS1cW0> NF7W[4g{cA>? NFnhU5NmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NY\4[2lZOjR|MEW2NFQ>
Huh7 NHn2Vm1CeG:ydH;zbZMhSXO|YYm= NXPtdYtNOm2P NYHxcVl1OTKq Mne3SG1UVw>? MmfqbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= NHXrWJgzPDJ7N{OwNC=>
Hep3B Ml7vRZBweHSxc3nzJGF{e2G7 MXGycW0> MWSxNog> M{D3SmROW09? MlTEbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= M{S2elI1Ojl5M{Cw
RKO NUnLR2ZwTnWwY4Tpc44hSXO|YYm= M1HHOFJuVQ>? Mn3ZNYg> M3HuS2ROW09? Mm\W[Y5p[W6lZYOgZ4VtdCCmZXH0bEBjgSCpZXzkZY5idXmlaX6= Mn;jNlQzQTF5N{e=
HepG2 E47 MWPGeY5kfGmxbjDBd5NigQ>? M4G5cVIvPW2P NXrBN2VZPDiq MoLmbY5kemWjc3XzJJRp\SC2b4jpZ4l1gSCxZjDBRUwhSlORLDDhcoQhS0OuNB?= MXqyOFI4Ozd|OB?=
LoVo  NH;Td2RCeG:ydH;zbZMhSXO|YYm= NYiwNG1rPW2P Mn;lOFhp M4LRW4VvcGGwY3XzJGRESS2rbnT1Z4VlKGGyb4D0c5Nqey5? NWLpSXZYOjR{MEG4NVI>
WiDr Ml7USpVv[3Srb36gRZN{[Xl? NF3HNFAyOG2P NE\6SWsycA>? MXnpcohq[mm2czDQR2JNNWmwZIXj[YQhVEN|IFnJJIV5eHKnc4Ppc44> MYCyOFE6ODR6OR?=
H1299  NILBRZJHfW6ldHnvckBCe3OjeR?= NHmyb3UyOG2P NEfaUZA1cA>? MmfsbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> M3W3TFI1OTd|MkC4
H460 NHi3elJHfW6ldHnvckBCe3OjeR?= NXzvdGN6OTCvTR?= NVXDeGZSPGh? M4fGeolv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NHiwSG0zPDF5M{KwPC=>
A549 NH;CfIVHfW6ldHnvckBCe3OjeR?= NEHFW3QyOG2P NWH5OFY{PGh? NX7lOI9jcW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliaYLyZYRq[XSrb36= MXiyOFE1Ojd|NR?=
Hep3B NUTSU4JJSXCxcITvd4l{KEG|c3H5 NEjZbXQ2dU1? Mnj0NlRp M{njO4F1fGWwdXH0[ZMhXE6ILd8xJJBzd3SnY4Tpc44h[WejaX7zeEB{\XK3bTDzeIFzfmG2aX;uMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NHPiV4kzPDB4Nk[5Ny=>
SMMC-7721 NEjvb|hCeG:ydH;zbZMhSXO|YYm= Mnv4OY1O NVKyN4IxOjSq NHjyS4RifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> M2TuRVI1ODZ4Nkmz
HO8910 MnvORZBweHSxc3nzJGF{e2G7 MVmxNI1O MkT4NVJp MWHlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> MlL0NlM6QDN4MUC=
MCF7 MVXGeY5kfGmxbjDBd5NigQ>? MkTlOY1O M3q5blI1cA>? NWXGTZkzcW6lcnXhd4V{KEO3TzDpcoR2[2WmIHPlcIwh\GWjdHi= MUCyN|k3OjZ{OR?=
HONE-1  M1XsZWZ2dmO2aX;uJGF{e2G7 NEDocnk2dU1? M1TmS|Fp MlexdoVxemW|c3XzJFZzNW2nZHnheIVlKFKRUzDwdo9lfWO2aX;u NXzRRoRjOjN6OUKzOVg>
HeLa NInZPG9HfW6ldHnvckBCe3OjeR?= MonJNVBuVQ>? NIKxbpQzcA>? MVnzeZBxemW|c3XzJGxEOyCLSTDlfJBz\XO|aYPvci=> NU[wNmxOOjN6NkS3N|g>
HepG2 MWXGeY5kfGmxbjDBd5NigQ>? MUixNI1O NXvITJkxOjSq MYPpcohq[mm2czDzbXRKT0GULTDhcoQhUEKVUz3pcoR2[2WmIHH1eI9xcGGpeR?= MWCyN|gyPzB2MB?=
SH-SY5Y MnvXSpVv[3Srb36gRZN{[Xl? M2TzWFFuVQ>? MXyyOIg> NFPwRWpqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgWG9EWA>? MX[yN|c1OzF2OB?=
SH-SY5Y MYLBdI9xfG:|aYOgRZN{[Xl? MUC1cW0> M4L5cFFp Mm\pZYJwdGm|aHXzJINmdGG|dILvcEBv\XW{b4Dyc5Rm[3SrdnWg[YZn\WO2 MnT2NlM3OTl|OUW=
PC12  NUO4fW5jTnWwY4Tpc44hSXO|YYm= MmXkNVBuVQ>? M2LGeFI1cA>? Mm\Re4F1\XJ? NH7JOYTDqGmwaHnibZR{KGOqeX3veJJ6eHOrbj3sbYtmKHC{b4TlZZNwdWGuIHHjeIl3cXS7Lh?= MV:yN|YxOzl5OR?=
OV2008 MofzRZBweHSxc3nzJGF{e2G7 M4nSdlVuVQ>? MWWyOIg> NFrsfYlkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= M2nmb|I{PTl{Mkix
A2780 NHXRW|JCeG:ydH;zbZMhSXO|YYm= NXjRb|AyPW2P MXKyOIg> NIn2WZZkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= MVGyN|U6OjJ6MR?=
Saos-2  M2TRUGFxd3C2b4Ppd{BCe3OjeR?= MUSxcW0> M{Lad|k3cA>? M1TTV4lv[3KnYYPld{Bk\WyuIHTlZZRpKGmwZIXj[YQh[nliUFPY NHnQdG4zOzV4M{G3NS=>
1321N1 M3nZcGN6fG:2b4jpZ4l1gSCDc4PhfS=> M4Xmb|VuVQ>? NX;Ke4hLOjSq MXTwdo91\WO2czDj[YxtKGGpYXnud5QhWEOQLXnu[JVk\WRidH;4bYNqfHl? MUSyN|UzPTJ4NR?=
SH-SY5Y NXHo[WtOS3m2b4TvfIlkcXS7IFHzd4F6 M13WNVVuVQ>? NGjOcJUzPGh? MV7pcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 M3PsbVI{PTJ3Mk[1
HT-29  MlH5SpVv[3Srb36gRZN{[Xl? MkDzNY1O NHznRmw1QC97Nni= MnXvbY5pcWKrdIOgRW1RUyCrbnT1Z4V{KGG3dH;wbIFocWNiY3XscEBl\WG2aB?= NV25NXBYOjN3MEiyO|I>
OR6 NH7hbYZHfW6ldHnvckBCe3OjeR?= NF7ae4IyOG2P NVK4W2RVPzKq Mn\Cd5VxeHKnc4Pld{BJS1ZicnXwcIlk[XSrb36gZY5lKG[xcn3heIlwdiCxZjDheZRweGijZ3;zc41mew>? NXz1NGtXOjN|OUW4O|U>
Hela  NX3aOIJJTnWwY4Tpc44hSXO|YYm= NGTpSWc2dU1? MkHTNlRp NILDPXFqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 NHj0XHAzOzN7NU[3PS=>
MCF-7  NVWxT4xKTnWwY4Tpc44hSXO|YYm= MUi1cW0> NFzUdmIzPGh? M3H0ZolvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> M1PyU|I{Ozl3Nke5
HUVECs MWXGeY5kfGmxbjDBd5NigQ>? NUfKdYU{O22P Mk[2NlRp NXPTSlNX[myxY3vzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBjgSCrbnjpZol1cW6pIHH1eI9xcGGpeR?= NWC1XYJ5OjN|NUi5Nlg>
T24 Ml7HSpVv[3Srb36gRZN{[Xl? M3n1PVExdU1? NFj4VVcycA>? NHvBU4lz\WS3Y3XzJJRp\SClbHXheoFo\SCxZjDMR|Mh[W[2ZYKgZoFq[2GuaX6geJJm[XSvZX70 MUCyN|M2PDB6MB?=
U251MG  NGfEZWlHfW6ldHnvckBCe3OjeR?= NXnPfYluO22P NFjwRmcycA>? MmfCd5VxeHKnc4Pld{BNSzNvSVmgdJJwfGWrbjDlfJBz\XO|aX;u NF3OcJQzOzN|OE[xPC=>
GTL-16  NYjEb44xSXCxcITvd4l{KEG|c3H5 MVO1cW0> NU\XSFY4OjSq MVTy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGG|IHPvcZBiemWmIITvJINmdGy|IITy[YF1\WRid3n0bEBOTVRiaX7obYJqfG:{cx?= Ml76NlM{OTN2OUC=
T-47D M1;I[mZ2dmO2aX;uJGF{e2G7 NFyzb3kyOG2P MlnENog> MXnpcohq[mm2czDheZRweGijZ4mgdJJw[2W|czDhcoQhcW6lcnXhd4V{KHKjcHHtfYNqdiCrbnT1Z4VlKGGyb4D0c5Nqew>? MXmyN|MxODB{Nh?=
PaCa44 M4PDNGFxd3C2b4Ppd{BCe3OjeR?= MYeyMlVuVQ>? MV:xbC=> MXHy[YR2[2W|wrDn[Y5qeGmwLX3l[IlifGWmIHHwc5B1d3Orcx?= NXHMdXdxOjNzMkSxNVI>
MDA-MB231 Ml2ySpVv[3Srb36gRZN{[Xl? M2PWbFVuVQ>? NFXuNnIycA>? MkHGbY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> MmXzNlMxQDh6NUC=
HeLa Mle1RZBweHSxc3nzJGF{e2G7 MVuxNI1O MknVNog> NVvZUpN6\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JINwNXS{ZXH0cYVvfCC5aYToJHBGUQ>? NYjjbpk3OjNyMECxN|U>
SK-HEP-1 M2nzPGFxd3C2b4Ppd{BCe3OjeR?= MmriNVBuVQ>? MV2xbC=> MVfwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz NXzRe4I5OjJ6NUi2OFk>
HepG2 MY\BdI9xfG:|aYOgRZN{[Xl? Mn\LN41O NEjvenA2cA>? NIPsfZVz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgVWR{ MY[yNlg{PjV7NR?=
MCF-7  NFz6eZFHfW6ldHnvckBCe3OjeR?= NW[4NId3Om2P NVLsSGh{OjSq Ml7zbY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhXE4EoB?= NXjES|BEOjR7N{C2O|Y>
MDA-MB-231 MVPGeY5kfGmxbjDBd5NigQ>? MmntNo1O NWThc49JOjSq M3vhPIlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= MUeyOFk4ODZ5Nh?=
MCF-7  M4PWZ2Z2dmO2aX;uJGF{e2G7 MXiycW0> NXzPUWlYPDiq NVS1[JAzeHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq MWSyOFk4ODZ5Nh?=
MDA-MB-231 NET3XopHfW6ldHnvckBCe3OjeR?= MormNo1O NXfPXGV2PDiq NXe3emZleHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NXHIVYo{OjR7N{C2O|Y>
PANC-1  M1HtVWFxd3C2b4Ppd{BCe3OjeR?= NYDqSm5yOW2P MnT4OFhp MnzwSG1UVw>? Mlyy[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> M1W0[lI1QDR{MUW4
MDA-MB 231 M1:1VWFxd3C2b4Ppd{BCe3OjeR?= NYTjW3BOPW2P M1\oUlAvPWh? M4S0Zo1w\HWuYYTld{BVd2OxbXnuxs7DqGmwZIXj[YQh[XCxcITvd4l{ MXqyOFg{ODd6MR?=
Microglia  NXP4eJZDSXCxcITvd4l{KEG|c3H5 NUXkfFZPPW2P NV\le5VwOjSq MUTk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MmryNlQ5OTh4MEG=
A2780cp MkfIRZBweHSxc3nzJGF{e2G7 NH;SfXYzNjWvTR?= NE[3c3oycA>? NEfWcIhl\Eh{Tx?= MmLs[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NELRdVQzPDhzN{m0Oi=>
HepG2 M2ewWmZ2dmO2aX;uJGF{e2G7 Mn35OY1O M4nzb|Rp MUXpcoNz\WG|ZYOgZ4VtdHWuYYKgcIV3\Wy|IH;mJGhNyqB? MnfXNlQ4OTN3OEe=
U2OS MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmroNVBuVQ>? NH\XcW0zPGh? MUHpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDEc5g> MUeyOFY{QTBzMx?=
HCT116 M{i0dGFxd3C2b4Ppd{BCe3OjeR?= M{Xud|VuVQ>? M1q0fFI1cA>? NUfXZ25{TE2VTx?= M{HUSYVvcGGwY3XzJIFxcWenbnnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MlThNlQ3OjZ3MkK=
MCF-7  NU\yNIJHSXCxcITvd4l{KEG|c3H5 Mn3kNE4ydU1? NV;uS2lKPmh? NVP3d4JV\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> M{\PO|IzPzVzOUi5
PC-3  MmjtRZBweHSxc3nzJGF{e2G7 Mly5No1O MYmybC=> MV7pcoNz\WG|ZYOgU3JKNWmwZIXj[YQh[2WubDDk[YF1cA>? M{DHe|IzPzR3NUiw
U251  NXzpSpk5SXCxcITvd4l{KEG|c3H5 MYm1cW0> NU\LWJY2OjSq MVrpcoNz\WG|ZYOgV|EucW6mdXPl[EBk\WyuIHTlZZRp M3jjRlIzPTd7N{i4
HeLa  MWjBdI9xfG:|aYOgRZN{[Xl? M{XodlVuVQ>? M2Pa[FI1cA>? NWL0NG1WcW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp MWOyNlU1PTF{OB?=
A549 M{DGbGZ2dmO2aX;uJGF{e2G7 NFjWUVMxNjGvTR?= MmjDNlRp NIezPHd{fXCycnXzd4V{KFOXMUGyO|QucW6mdXPl[EBk\WyuIHTlZZRp NXLiWVFsOjJ2Nk[5OlA>
pDCs NGPkWWNHfW6ldHnvckBCe3OjeR?= MYKxNI1O NV;vU2J2OC53aB?= MYpCpJJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDJSm4u|rFiYomgd5NTVkF2MB?= M{Wzd|IzOzl4NUm5
BGC-823 M3\YS2Z2dmO2aX;uJGF{e2G7 M371UFVuVQ>? Mor6Nog> NFLFNVBqdmirYnn0d{B1cGVicnH0[UBw\iCjdYTvdIhi\2mlIHPlcIx{ NYrpXFJxOjJ|MkKxOVI>
U937 NEnzbnBHfW6ldHnvckBCe3OjeR?= Mkj4No1O M{PtbFEzcA>? MWjk[YNz\WG|ZYOgeIhmKGG3dH;wbIFogSC{YYTpc:Kh NYTXXodkOjJzNUWxOVA>
Marc-145 MomzSpVv[3Srb36gRZN{[Xl? MUi1cW0> MWexNk8zPC9|Nni= NXK3e5dvemWmdXPld{B1cGViUGLSV3YhfGm2ZYLzJIFv\CC2aHWgdJJwfGWrbjDlfJBz\XO|aX;u NYjo[244OjJzMUm5NFA>
HBx  Mn3aRZBweHSxc3nzJGF{e2G7 NGP0c3AyOG2P M13kNlQ5cA>? NWPxW4NuTE2VTx?= NG\RNnVqdmO{ZXHz[ZMh[2WubDDk[YF1cA>? Mmm3NlIxOjByN{i=
MCF-7 NGDkRlZHfW6ldHnvckBCe3OjeR?= MXSxNI1O MYS0PIg> NEi4TZNjdG:la4OgZZV1d3CqYXf5JIlv\HWlZXSgZpkh[m:{dHX6c41q[g>? MnmyNlE6OzF7M{e=
RMPI8226  NH\0N2xHfW6ldHnvckBCe3OjeR?= MoK2OY1O MknHNYg> NH;tfoF{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w NUO0UIlZOjF7MUW2NlA>
PC12/TetOn NFPmRVRHfW6ldHnvckBCe3OjeR?= NVLkZ|VDOC5zL{HtUS=> MoDPNVhp MYLs[YFleyC2bzFOtU1{gW5qV2SpJIFk[3WvdXzheIlwdixidH;4bYNqfHluIHHu[EBwdGmpb33ldkBnd3KvYYTpc47DqA>? MkOzNlE6ODZ4NUm=
HeLa  NF\ZTpZEgXSxdH;4bYNqfHliQYPzZZk> NVG0RVI2Om2P M{Hve|I1cA>? MVPpcohq[mm2ZYOgeIhmKGO7dH;0c5hq[2m2eTDv[kB{cWyrYnnubY4hfG9iSHXMZUBk\Wyucz6= NWP4[W05OjF6N{WzPFU>
Jurkat M2nmRmZ2dmO2aX;uJGF{e2G7 NHXqV|MyOG2P M2faV|Fp M37UfYRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= NYHtNZpzOjF6NkSwN|c>
K562 MWnGeY5kfGmxbjDBd5NigQ>? NIPyb2MyOG2P MnfuNYg> M1\6WoRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= NXflOVBLOjF6NkSwN|c>
Nara-H M1j5[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWO1cW0> MXm0PIg> NYfTfXNO\W6qYX7j[ZMhfGWvc3nyc4xqdXW|bXXkbYF1\WRic4XwdJJme3Orb36gc4YhVmG{YT3IJINmdGxicILvcIln\XKjdHnvci=> M1LXRlIyQDB3MEOz
HUVECs NFTNe5dHfW6ldHnvckBCe3OjeR?= MVuxNI1O NX[0fHFXOC53aB?= M3fKS4Rm[3KnYYPld{B1cGViQVfFMWJUSWmwZIXj[YQh[XW2b4DoZYd6KGyndnW= NYXhO5RUOjF2Nki1PVI>
HepG2 NETGcYtCeG:ydH;zbZMhSXO|YYm= NGTw[WczdU1? MX2xbC=> Mn;r[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> M{\JUlIyPDV|Nkmx

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

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Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID