3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. Solutions of 3-MA are best fresh-prepared by heating.

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5 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    After getting treated with 10 μM compound C for indicated time periods with or without 50 μM 3-MA (C) pre-treatment for 1 h, the levels of indicated proteins in QBC939 and RBE cells were analyzed using Western blot.

    J Cell Biochem, 2017, 119(7):5538-5550. 3-Methyladenine (3-MA) purchased from Selleck.

  • A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. Solutions of 3-MA are best fresh-prepared by heating.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D MmPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIO2co4{dU1? M3TkfVJp M2nvUGROW09? MnfxdoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? MnvzNlU4OTZ3NkG=
A549  MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnrcHEzO22P NGHJXpIzcA>? M4PKcmROW09? M3zmOZJm\HWlZYOg[5Jwf3SqIHnubIljcXSxcomg[YZn\WO2IH;mJGJFVUN? MnzENlU4OTZ3NkG=
95D MoLYRZBweHSxc3nzJGF{e2G7 NHviWoY2dU1? NYq5ZVlwOmh? MmjXSG1UVw>? MlrTbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq NUP1NWZTOjV5MU[1OlE>
A549  M2XYNmFxd3C2b4Ppd{BCe3OjeR?= NUTPUYdTPW2P MXiybC=> NWnFR3dDTE2VTx?= NWDiVlh3cW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo MnjYNlU4OTZ3NkG=
NBL-W-S  MofjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrNNY1O NH3kZmo3cA>? M{\PdYVvcGGwY3XzJGdCVlRvNkGgeI95cWOrdIm= NInLUmEzPTN{M{KyNi=>
NBL-W-S  NVnHSXFrSXCxcITvd4l{KEG|c3H5 M1PsOlFuVQ>? Mn7QOog> NWnhTGxicW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhT0GQVD22NS=> MWKyOVMzOzJ{Mh?=
A2780cp  M4DLfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWexcW0> MmrJNYg> NIjaZWhqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NWDrOnRROjV|MkK2PVQ>
A549  NInWWYNHfW6ldHnvckBCe3OjeR?= NIHhdWo2dU1? M3vEOlJp M2njcpN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> NF;W[5MzPTJ6NU[yPC=>
H157 M1\jZ2Z2dmO2aX;uJGF{e2G7 NIHL[5Y2dU1? MXOybC=> MVrzeZBxemW|c3XzJHNRSyCrbnT1Z4VlKGGlY4XteYxifGmxbjDv[kBNSzNvSVm= NU[y[IZZOjV{OEW2Nlg>
MDR MWHBdI9xfG:|aYOgRZN{[Xl? MXWxNI1O NWfnRlRwPmh? NEnXeZF{fHKnbnf0bIVveyC2aHWgdI94\XJib3[gZY51cWOjbnPldkBlenWpcx?= MkToNlUxOTl5MEG=
HT-29  NH\sOo9CeG:ydH;zbZMhSXO|YYm= MXWxcW0> MoHVOFhp NVLLOodsTE2VTx?= M1nmfYVvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NWXXd25jOjR6NEKxOVg>
Microglia  MXPBdI9xfG:|aYOgRZN{[Xl? M1i4UlVuVQ>? M1joTlI1cA>? MnzR[IVkemWjc3XzJIh6eG:6aXGtbY5lfWOnZDDj[YxtKGSnYYTo NVO3W2N1OjR6MUi2NFE>
A2780cp MX7BdI9xfG:|aYOgRZN{[Xl? MXOyMlVuVQ>? MUCxbC=> MWDk[GgzVw>? Mnr2[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NEC2SpozPDhzN{m0Oi=>
HepG2 NFru[pRHfW6ldHnvckBCe3OjeR?= MXK1cW0> NHn6bpQ1cA>? M{H3S4lv[3KnYYPld{BJVCC{ZXzlZZNm MnriNlQ4OTN3OEe=
A549 MmeyRZBweHSxc3nzJGF{e2G7 MlviOY1O M3jBWVQ5cA>? NFji[Xhl\WO{ZXHz[ZMhfGinIIDldoNmdnSjZ3Wgc4Yh[2WubDDk[YF1cCCjbnSg[ZhxemW|c3nvckBt\X[nbIOgc4Yh[2G|cHHz[U0{NCCEZXPsbY4uOSCjbnSgUGM{NUmL MX[yOFcxPjNyMx?=
U2OS NIOwXIdCeG:ydH;zbZMhSXO|YYm= M4XM[|ExdU1? M3rEb|I1cA>? MVLpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MV:yOFY{QTBzMx?=
Saos-2 MnHORZBweHSxc3nzJGF{e2G7 NFjX[mkyOG2P Mm\ENlRp MV7pcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MonNNlQ3OzlyMUO=
A549 MWDBdI9xfG:|aYOgRZN{[Xl? M1TRcFExdU1? Mn74OFhp M{mzbIFk[2WuZYLheIV{KHSqZTDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBRXFh? MYSyOFYzPjd{Mh?=
HCT116  MlS2RZBweHSxc3nzJGF{e2G7 NIfufXc2dU1? NXzSOYg{OjSq M{PseoVvcGGwY3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDhdIlo\W6rbh?= Ml\6NlQ3OjZ3MkK=
HGC-27 MljZSpVv[3Srb36gRZN{[Xl? M3\UUVExdU1? NF7GXIoycA>? M4\JXIlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBtd3O|IHL5JHJCTDByMTDvdkBOUy1{MkC2 NWmyOnVQOjR2MU[zOFk>
U2OS  NY\VRYpRTnWwY4Tpc44hSXO|YYm= MkDwNE42NzGvTR?= NFft[JI1QGh? MUTpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> NGr3[VczPDN3OEO0Ni=>
MG-63 MWXGeY5kfGmxbjDBd5NigQ>? M1zQO|AvPS9zbV2= MV[0PIg> MnWxbY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> MnLxNlQ{PTh|NEK=
MG-63 NFrFfoFCeG:ydH;zbZMhSXO|YYm= MXmwMlUwOW2P MVqzNog> MorT[Y5p[W6lZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= Mn;zNlQ{PTh|NEK=
MG-63 NYf5NItFSXCxcITvd4l{KEG|c3H5 M{Llb|ExdU1? MoexNVJp Mkjp[Y5p[W6lZYOgSHAucW6mdXPl[EBieG:ydH;zbZM> Mkj1NlQ{PTh|MEG=
NTUB1 M172[2Fxd3C2b4Ppd{BCe3OjeR?= M3PXRlVuVQ>? MXWxMlVp Mly1dI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{ MlnlNlQ{PDlzN{[=
T24 MYrBdI9xfG:|aYOgRZN{[Xl? M3\HUVVuVQ>? NFLjbYMyNjWq M2nncZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? NHztTZczPDN2OUG3Oi=>
SGC-7901  MXfBdI9xfG:|aYOgRZN{[Xl? NE\jb5MzdU1? MYexbC=> NUP6S2FncW6lcnXhd4V{KEODLUSgbY5lfWOnZDDhdI9xfG:|aYO= MmnXNlQ{OjF|NEC=
SMMC-7721 MY\BdI9xfG:|aYOgRZN{[Xl? M1j1VlJuVQ>? NWnWNnFIOWh? NFjpfI5qdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= NELDRXMzPDN{MUO0NC=>
ECSCs  M13Vc2Fxd3C2b4Ppd{BCe3OjeR?= NGrBPYUyOG2P NH;SN3U1cA>? NX3QfWY3\GWlcnXhd4V{KHKjcHHtfYNqdi22cnXheIVlKGGyb4D0c5Nqew>? MVOyOFMyQTFyOR?=
MCF-7  NEjtN21HfW6ldHnvckBCe3OjeR?= MojJNVBuVQ>? M1XiO|I1cA>? NIPzXZRqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgZ4hmdW:2aHXyZZB6KGS{dXfz NYrMTVFuOjR|MUW1O|g>
UOK257 NXXpcHM5SXCxcITvd4l{KEG|c3H5 MWS1cW0> MV2zbC=> MXXlcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh NGTLWmozPDNyNU[wOC=>
ACHN-5968 NEDnfZZCeG:ydH;zbZMhSXO|YYm= M3jBbFVuVQ>? MWezbC=> MXflcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh M4r6OFI1OzB3NkC0
Huh7 NH;GNlhCeG:ydH;zbZMhSXO|YYm= NInyOWEzdU1? M2nSS|EzcA>? MojNSG1UVw>? NIXDU3NqdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MlfYNlQzQTd|MEC=
Hep3B NGDod5hCeG:ydH;zbZMhSXO|YYm= NVvHVWtjOm2P MWmxNog> NUO3b3M5TE2VTx?= M13Yd4lvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo MlzqNlQzQTd|MEC=
RKO NI\qU2FHfW6ldHnvckBCe3OjeR?= M2D3cVJuVQ>? MWKxbC=> Mnq5SG1UVw>? MV;lcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= MWiyOFI6OTd5Nx?=
HepG2 E47 MVfGeY5kfGmxbjDBd5NigQ>? M4LZNVIvPW2P MXm0PIg> NVPVbm86cW6lcnXhd4V{KHSqZTD0c5hq[2m2eTDv[kBCSSxiQmPPMEBidmRiQ1PsOC=> NVjIcGVzOjR{N{O3N|g>
LoVo  Mnr0RZBweHSxc3nzJGF{e2G7 NEe1R3I2dU1? NX20cWQzPDiq MmX0[Y5p[W6lZYOgSGNCNWmwZIXj[YQh[XCxcITvd4l{Ng>? MlPmNlQzODF6MUK=
WiDr NV\td4ZtTnWwY4Tpc44hSXO|YYm= MX[xNI1O NH3WWWUycA>? NYXZfm5McW6qaXLpeJMhWEOETD3pcoR2[2WmIFzDN{BKUSCneIDy[ZN{cW:w MoPRNlQyQTB2OEm=
H1299  NFr4SZFHfW6ldHnvckBCe3OjeR?= MnTZNVBuVQ>? MYW0bC=> NFjpTGNqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NWHjZVFzOjRzN{OyNFg>
H460 M1rDPGZ2dmO2aX;uJGF{e2G7 NVfC[W5GOTCvTR?= NFm2fmM1cA>? MV;pcoNz\WG|ZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NGjkXZMzPDF5M{KwPC=>
A549 NWLkfot6TnWwY4Tpc44hSXO|YYm= MkSwNVBuVQ>? MlXIOIg> NHnkZZRqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCrcoLh[IlifGmxbh?= NWTNXWQ5OjRzNEK3N|U>
Hep3B NGXoVZlCeG:ydH;zbZMhSXO|YYm= MX:1cW0> M1zTTlI1cA>? NUjTd3F[[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ MlnUNlQxPjZ4OUO=
SMMC-7721 MXfBdI9xfG:|aYOgRZN{[Xl? NEnCSpI2dU1? Mof5NlRp MYLheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= MmTSNlQxPjZ4OUO=
HO8910 MnfJRZBweHSxc3nzJGF{e2G7 Mlz4NVBuVQ>? M2[3OFEzcA>? MYHlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> MYGyN|k5OzZzMB?=
MCF7 NIKyNpVHfW6ldHnvckBCe3OjeR?= NGHscWg2dU1? NFu3SG4zPGh? MkPqbY5kemWjc3XzJGN2VyCrbnT1Z4VlKGOnbHyg[IVifGh? NYjKW3FYOjN7NkK2Nlk>
HONE-1  NUXGeGNmTnWwY4Tpc44hSXO|YYm= Mnv1OY1O NULvbI9YOWh? NUGwSoxNemWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v MkjSNlM5QTJ|NUi=
HeLa NX;rUmliTnWwY4Tpc44hSXO|YYm= NWrlXY55OTCvTR?= NWT6[lRVOmh? M1\OUJN2eHC{ZYPz[ZMhVEN|IFnJJIV5eHKnc4Ppd49v MWSyN|g3PDd|OB?=
HepG2 MYLGeY5kfGmxbjDBd5NigQ>? MoDsNVBuVQ>? NE\BNoUzPGh? NHnIcm9qdmirYnn0d{B{cVSLR1HSMUBidmRiSFLTV{1qdmS3Y3XkJIF2fG:yaHHnfS=> MkjZNlM5OTdyNEC=
SH-SY5Y MoLxSpVv[3Srb36gRZN{[Xl? M1:0dVFuVQ>? MkjyNlRp Mke4bY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IGTPR3A> MViyN|c1OzF2OB?=
SH-SY5Y NUTFR|dFSXCxcITvd4l{KEG|c3H5 MlSyOY1O M4XEflFp NUf2SnBl[WKxbHnzbIV{KGOnbHHzeJJwdCCwZYXyc5Bzd3SnY4TpeoUh\W[oZXP0 MkjlNlM3OTl|OUW=
PC12  M4LxO2Z2dmO2aX;uJGF{e2G7 MXSxNI1O MnjMNlRp MYr3ZZRmeg>? MVdCpIlvcGmkaYTzJINpgW2xdIL5dJNqdi2uaXvlJJBzd3SnYYPvcYFtKGGldHn2bZR6Ng>? NX;JTGV6OjN4MEO5O|k>
OV2008 NInzdYZCeG:ydH;zbZMhSXO|YYm= MYi1cW0> MlzPNlRp M3i1PINwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| MXmyN|U6OjJ6MR?=
A2780 NXTNNYxsSXCxcITvd4l{KEG|c3H5 M2jnXVVuVQ>? MV:yOIg> MlTZZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? MnvZNlM2QTJ{OEG=
Saos-2  M4nl[mFxd3C2b4Ppd{BCe3OjeR?= M4XDOVFuVQ>? NGrLcVg6Pmh? MmPUbY5kemWjc3XzJINmdGxiZHXheIghcW6mdXPl[EBjgSCSQ2i= M4XURVI{PTZ|MUex
1321N1 MmH2R5l1d3SxeHnjbZR6KEG|c3H5 NXXBOJZJPW2P NHK4VYUzPGh? MnHWdJJwfGWldIOgZ4VtdCCjZ3HpcpN1KFCFTj3pcoR2[2WmIITvfIlkcXS7 MkLxNlM2OjV{NkW=
SH-SY5Y NWTzeoJwS3m2b4TvfIlkcXS7IFHzd4F6 NIfKb3o2dU1? MkDnNlRp NF7yZZRqdmO{ZXHz[ZMhWEOQIITvfIlkcXS7 M37DS|I{PTJ3Mk[1
HT-29  NUX1clZyTnWwY4Tpc44hSXO|YYm= M2i0e|FuVQ>? NHPLdVY1QC97Nni= MYrpcohq[mm2czDBUXBMKGmwZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSq Mo\1NlM2ODh{N{K=
OR6 M1;G[GZ2dmO2aX;uJGF{e2G7 NWPoc4dIOTCvTR?= NFzpcog4Omh? NIDNT2t{fXCycnXzd4V{KEiFVjDy[ZBtcWOjdHnvckBidmRiZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MXqyN|M6PTh5NR?=
Hela  MVPGeY5kfGmxbjDBd5NigQ>? M2C4OFVuVQ>? MkLsNlRp MnHEbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> M4LFVlI{Ozl3Nke5
MCF-7  MULGeY5kfGmxbjDBd5NigQ>? MWi1cW0> MXKyOIg> NXfQSZdYcW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? MkjoNlM{QTV4N{m=
HUVECs NFLwT21HfW6ldHnvckBCe3OjeR?= MYKzcW0> MlHWNlRp NVPHcIJ7[myxY3vzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBjgSCrbnjpZol1cW6pIHH1eI9xcGGpeR?= MYOyN|M2QDl{OB?=
T24 NFjNZlFHfW6ldHnvckBCe3OjeR?= M1rlN|ExdU1? M1zre|Fp MYfy[YR2[2W|IITo[UBkdGWjdnHn[UBw\iCOQ{OgZYZ1\XJiYnHpZ4FtcW5idILlZZRu\W62 MoHNNlM{PTRyOEC=
U251MG  MVHGeY5kfGmxbjDBd5NigQ>? M1jncFNuVQ>? MUWxbC=> Ml7Yd5VxeHKnc4Pld{BNSzNvSVmgdJJwfGWrbjDlfJBz\XO|aX;u M3zmO|I{OzN6NkG4
GTL-16  M2O0UGFxd3C2b4Ppd{BCe3OjeR?= NULuZXRDPW2P NH\uclQzPGh? MmPKdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= NFPmSW0zOzNzM{S5NC=>
T-47D MoO3SpVv[3Srb36gRZN{[Xl? M1zET|ExdU1? M3zDZ|Jp MmDObY5pcWKrdIOgZZV1d3CqYXf5JJBzd2Onc4OgZY5lKGmwY4LlZZNmeyC{YYDhcZlkcW5iaX7keYNm\CCjcH;weI9{cXN? NX\CRohbOjN|MECwNlY>
PaCa44 M3vuR2Fxd3C2b4Ppd{BCe3OjeR?= MmPuNk42dU1? MYixbC=> MVny[YR2[2W|wrDn[Y5qeGmwLX3l[IlifGWmIHHwc5B1d3Orcx?= MWWyN|EzPDFzMh?=
MDA-MB231 MXvGeY5kfGmxbjDBd5NigQ>? MV61cW0> MkDNNYg> MmrkbY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> NG\ndW8zOzB6OEi1NC=>
HeLa NGKyb|hCeG:ydH;zbZMhSXO|YYm= MWmxNI1O NGW0e4ozcA>? MkjT[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2bXXueEB4cXSqIGDFTS=> MmS1NlMxODBzM{W=
SK-HEP-1 Mlr2RZBweHSxc3nzJGF{e2G7 NYTncmRHOTCvTR?= M4DTRlFp MUPwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz M4X1SlIzQDV6NkS5
HepG2 MWjBdI9xfG:|aYOgRZN{[Xl? NH;2RY4{dU1? M17DSlVp M4jkS5Jm\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDRSJM> MXeyNlg{PjV7NR?=
MCF-7  MXfGeY5kfGmxbjDBd5NigQ>? M1PSWlJuVQ>? M3z6dlI1cA>? MWjpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NFzRdHYzPDl5ME[3Oi=>
MDA-MB-231 M1PVVmZ2dmO2aX;uJGF{e2G7 NV3BN4p3Om2P NG\sW4IzPGh? M1n4TIlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= M{jsPFI1QTdyNke2
MCF-7  MVTGeY5kfGmxbjDBd5NigQ>? MXiycW0> M4PRfFQ5cA>? NWjZb5lWeHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NGfMZ4czPDl5ME[3Oi=>
MDA-MB-231 MmTrSpVv[3Srb36gRZN{[Xl? MWeycW0> MkT6OFhp MXnwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> MmfwNlQ6PzB4N{[=
PANC-1  MoK3RZBweHSxc3nzJGF{e2G7 M33lRVFuVQ>? MUC0PIg> MVrEUXNQ MV\lcohidmOnczDic5J1\XqxbXniMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> NEj5doszPDh2MkG1PC=>
MDA-MB 231 MmP1RZBweHSxc3nzJGF{e2G7 MmXPOY1O NUHSeI4{OC53aB?= M4e3cI1w\HWuYYTld{BVd2OxbXnuxs7DqGmwZIXj[YQh[XCxcITvd4l{ NEHCNHEzPDh|MEe4NS=>
Microglia  MlXpRZBweHSxc3nzJGF{e2G7 MkLLOY1O MVyyOIg> MWrk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MXOyOFgyQDZyMR?=
A2780cp M3ziPGFxd3C2b4Ppd{BCe3OjeR?= MlnRNk42dU1? Mn75NYg> NYewWWFZ\GSKMl:= MWPlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo NIXTRlIzPDhzN{m0Oi=>
HepG2 M1LZNWZ2dmO2aX;uJGF{e2G7 M4[2XFVuVQ>? NEHlb3o1cA>? NWi0R4ZpcW6lcnXhd4V{KGOnbHz1cIFzKGyndnXsd{Bw\iCKTNMg M2D3bFI1PzF|NUi3
U2OS NVj0NINmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jhUFExdU1? MW[yOIg> NWfYU2RzcW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJIlv\HWlZXSgZpkhTG:6 M2rlc|I1PjN7MEGz
HCT116 NEDFXHZCeG:ydH;zbZMhSXO|YYm= NELR[Hg2dU1? MnLRNlRp Mn7LSG1UVw>? MYnlcohidmOnczDhdIlo\W6rbj3pcoR2[2WmIHPlcIwh\GWjdHi= M4PZblI1PjJ4NUKy
MCF-7  NYLnW5hoSXCxcITvd4l{KEG|c3H5 NFnRNVIxNjGvTR?= MVm2bC=> NXfL[lUx\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> MlnlNlI4PTF7OEm=
PC-3  M4G3NGFxd3C2b4Ppd{BCe3OjeR?= MmLDNo1O MWCybC=> NInkbo1qdmO{ZXHz[ZMhV1KLLXnu[JVk\WRiY3XscEBl\WG2aB?= NVHkb25NOjJ5NEW1PFA>
U251  NEjQNnhCeG:ydH;zbZMhSXO|YYm= NVzWZYhMPW2P NV3aeFhKOjSq M{nwfYlv[3KnYYPld{BUOS2rbnT1Z4VlKGOnbHyg[IVifGh? MYmyNlU4QTd6OB?=
HeLa  NY[4dYtGSXCxcITvd4l{KEG|c3H5 NFzEOoo2dU1? MoPPNlRp NHOz[WxqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> M{P5VFIzPTR3MUK4
A549 M4TVWWZ2dmO2aX;uJGF{e2G7 NY\6N3p5OC5zbV2= MWqyOIg> MWLzeZBxemW|c3XzJHNWOTF{N{StbY5lfWOnZDDj[YxtKGSnYYTo MVGyNlQ3Pjl4MB?=
pDCs MVPGeY5kfGmxbjDBd5NigQ>? MYexNI1O MnXyNE42cA>? MkXXxsBz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gTWZPNc7zIHL5JJN{Wk6DNEC= MlXTNlI{QTZ3OUm=
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U937 NVPiXnM{TnWwY4Tpc44hSXO|YYm= MlK1No1O MYGxNog> M3rzTYRm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? MkHKNlIyPTVzNUC=
Marc-145 MnX4SpVv[3Srb36gRZN{[Xl? MYK1cW0> NUixeHQ5OTJxMkSvN|Zp M3jUWpJm\HWlZYOgeIhmKFCUUmPWJJRqfGW{czDhcoQhfGinIIDyc5RmcW5iZYjwdoV{e2mxbh?= MWeyNlEyQTlyMB?=
HBx  MlXlRZBweHSxc3nzJGF{e2G7 NVPQeVZDOTCvTR?= NWr6Zlc4PDiq NGrVZ5BFVVOR MXjpcoNz\WG|ZYOgZ4VtdCCmZXH0bC=> NFLqNW4zOjB{MEC3PC=>
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RMPI8226  NX:zd|N1TnWwY4Tpc44hSXO|YYm= MUS1cW0> MYmxbC=> NHfOeI9{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w MlTzNlE6OTV4MkC=
PC12/TetOn Mni5SpVv[3Srb36gRZN{[Xl? M3;YPVAvOS9zbV2= NELHNVgyQGh? MYDs[YFleyC2bzFOtU1{gW5qV2SpJIFk[3WvdXzheIlwdixidH;4bYNqfHluIHHu[EBwdGmpb33ldkBnd3KvYYTpc47DqA>? NFj1PW8zOTlyNk[1PS=>
HeLa  NGLuSW1EgXSxdH;4bYNqfHliQYPzZZk> NYfHfoM5Om2P NGHCOIIzPGh? NULQfW5RcW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv MojhNlE5PzV|OEW=
Jurkat MV;GeY5kfGmxbjDBd5NigQ>? MnjtNVBuVQ>? MnqzNYg> M3vnToRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= NW\NWpNMOjF6NkSwN|c>
K562 MVzGeY5kfGmxbjDBd5NigQ>? MlzFNVBuVQ>? M2nRb|Fp Mo\o[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NUTZXXVWOjF6NkSwN|c>
Nara-H NEe1VYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjremZZPW2P NVLjeVN3PDiq NHPxXoVmdmijbnPld{B1\W2|aYLvcIlufXOvZXTpZZRm\CC|dYDwdoV{e2mxbjDv[kBP[XKjLVigZ4VtdCCycn;sbYZmemG2aX;u NUmz[YJyOjF6MEWwN|M>
HUVECs MUfGeY5kfGmxbjDBd5NigQ>? MmDpNVBuVQ>? MoS3NE42cA>? MmO3[IVkemWjc3XzJJRp\SCDR1WtRnNCcW6mdXPl[EBifXSxcHjh[5khdGW4ZR?= M37Q[lIyPDZ6NUmy
HepG2 M1;z[GFxd3C2b4Ppd{BCe3OjeR?= NULTfXdwOm2P M3PEWVFp MnLP[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NUfxdYd3OjF2NUO2PVE>

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
in solvent
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID