3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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5 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    After getting treated with 10 μM compound C for indicated time periods with or without 50 μM 3-MA (C) pre-treatment for 1 h, the levels of indicated proteins in QBC939 and RBE cells were analyzed using Western blot.

    J Cell Biochem, 2017, 119(7):5538-5550. 3-Methyladenine (3-MA) purchased from Selleck.

  • A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D Mnz5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrJfZE{dU1? MXeybC=> M4TwWWROW09? MWHy[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E NYW0eW5IOjV5MU[1OlE>
A549  NWnMWpV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\3[FNuVQ>? M2eyfFJp MX;EUXNQ MVny[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E M2jhZ|I2PzF4NU[x
95D MlvSRZBweHSxc3nzJGF{e2G7 MVm1cW0> M4TU[|Jp NV\ocnNNTE2VTx?= MmrmbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq Ml35NlU4OTZ3NkG=
A549  MkjTRZBweHSxc3nzJGF{e2G7 NHTneIM2dU1? MoPZNog> NG\jcIdFVVOR M4XJb4lvcGmkaYTzJGJFVUNvaX7keYNm\CCjcH;weI91cWNiY3XscEBl\WG2aB?= M3vlZlI2PzF4NU[x
NBL-W-S  M2XOU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXOxcW0> NEXj[JM3cA>? NVPHeJpy\W6qYX7j[ZMhT0GQVD22NUB1d3irY3n0fS=> NGDtXoMzPTN{M{KyNi=>
NBL-W-S  NGq1VWtCeG:ydH;zbZMhSXO|YYm= NWjNWmZmOW2P MlWzOog> Moe2bY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgS2FPXC14MR?= MYeyOVMzOzJ{Mh?=
A2780cp  M2Lkbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLkNY1O M3jsN|Fp NW\MUXpQcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? M2Xje|I2OzJ{Nkm0
A549  NYHPd3ZLTnWwY4Tpc44hSXO|YYm= NWnTepp{PW2P NHPUTHkzcA>? M3P1RZN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> MkHxNlUzQDV4Mki=
H157 NX\M[oFRTnWwY4Tpc44hSXO|YYm= MVe1cW0> M3TQTlJp MnnKd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ NIm5fIYzPTJ6NU[yPC=>
MDR M4jlS2Fxd3C2b4Ppd{BCe3OjeR?= MmXsNVBuVQ>? M3:4eVZp M4qyZpN1emWwZ4To[Y5{KHSqZTDwc5dmeiCxZjDhcpRq[2GwY3XyJIRzfWe| NEnZZ5czPTBzOUewNS=>
HT-29  M1zwV2Fxd3C2b4Ppd{BCe3OjeR?= M1nJclFuVQ>? NXnI[WtSPDiq MnTlSG1UVw>? NYDKSHVV\W6qYX7j[ZMh[m:{dHX6c41q[i2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? M2XaPFI1QDR{MUW4
Microglia  MmfzRZBweHSxc3nzJGF{e2G7 MlnwOY1O MnToNlRp MWDk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= NHvxbYgzPDhzOE[wNS=>
A2780cp MXTBdI9xfG:|aYOgRZN{[Xl? NGnZV|gzNjWvTR?= M2XudFFp NHzzW4Zl\Eh{Tx?= MXflcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo NYTaeIp{OjR6MUe5OFY>
HepG2 NX;hbFRFTnWwY4Tpc44hSXO|YYm= M3f2TFVuVQ>? NWj0e4tRPGh? MnTLbY5kemWjc3XzJGhNKHKnbHXhd4U> NGLYNlEzPDdzM{W4Oy=>
A549 MoHXRZBweHSxc3nzJGF{e2G7 NYXEe41jPW2P M1zJWVQ5cA>? MoTp[IVkemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGxiZHXheIgh[W6mIHX4dJJme3Orb36gcIV3\Wy|IH;mJINie3Cjc3WtN{whSmWlbHnuMVEh[W6mIFzDN{1KUQ>? M4\Bc|I1PzB4M{Cz
U2OS M1PzemFxd3C2b4Ppd{BCe3OjeR?= MVKxNI1O NV7ue5ZHOjSq NX7MUIQ2cW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 NHnOWm4zPDZ|OUCxNy=>
Saos-2 NH3LXIRCeG:ydH;zbZMhSXO|YYm= MmewNVBuVQ>? MojSNlRp M1vvRYlvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? MmPJNlQ3OzlyMUO=
A549 NG\2[FdCeG:ydH;zbZMhSXO|YYm= MX2xNI1O NVrV[Jp4PDiq NY\UOFk5[WOlZXzldoF1\XNidHjlJJJm\HWldHnvckBw\iClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KFCWWB?= NVL4dINHOjR4Mk[3NlI>
HCT116  NYP1TIdiSXCxcITvd4l{KEG|c3H5 MkTsOY1O NWXoeG1HOjSq M4jNO4VvcGGwY3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDhdIlo\W6rbh?= M{TuflI1PjJ4NUKy
HGC-27 NEntUmZHfW6ldHnvckBCe3OjeR?= MnzCNVBuVQ>? MYOxbC=> MUDpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgcI9{eyCkeTDSRWQxODFib4KgUWsuOjJyNh?= MkDINlQ1OTZ|NEm=
U2OS  MXPGeY5kfGmxbjDBd5NigQ>? MoTyNE42NzGvTR?= NFfvc4I1QGh? NYXpZVE{cW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? NYHlVoQ3OjR|NUizOFI>
MG-63 M2\wSmZ2dmO2aX;uJGF{e2G7 NE\mbmkxNjVxMX3N MXu0PIg> NFLUdHpqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NIjwOoIzPDN3OEO0Ni=>
MG-63 MU\BdI9xfG:|aYOgRZN{[Xl? NV7t[3EzOC53L{HtUS=> MVKzNog> M3\jSoVvcGGwY3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= NFvK[48zPDN3OEO0Ni=>
MG-63 MUfBdI9xfG:|aYOgRZN{[Xl? MVGxNI1O MlHBNVJp MYPlcohidmOnczDEVE1qdmS3Y3XkJIFxd3C2b4Ppdy=> NXG1fY5[OjR|NUizNFE>
NTUB1 M2TTOWFxd3C2b4Ppd{BCe3OjeR?= NXnNXWpZPW2P MmrzNU42cA>? M{PFWZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? MmPvNlQ{PDlzN{[=
T24 MVTBdI9xfG:|aYOgRZN{[Xl? MYm1cW0> MnnYNU42cA>? M363WZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? M4[1WVI1OzR7MUe2
SGC-7901  M{HhSWFxd3C2b4Ppd{BCe3OjeR?= MnTzNo1O NVHJcnhkOWh? NYXVVlV[cW6lcnXhd4V{KEODLUSgbY5lfWOnZDDhdI9xfG:|aYO= NGnpTZMzPDN{MUO0NC=>
SMMC-7721 M2TjbmFxd3C2b4Ppd{BCe3OjeR?= MXmycW0> NYL4bXV2OWh? NHjsemdqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= Mn;rNlQ{OjF|NEC=
ECSCs  NXiwVWhDSXCxcITvd4l{KEG|c3H5 MXixNI1O MoDDOIg> M1e3coRm[3KnYYPld{Bz[XCjbYnjbY4ufHKnYYTl[EBieG:ydH;zbZM> MVGyOFMyQTFyOR?=
MCF-7  MnnJSpVv[3Srb36gRZN{[Xl? M{jWVFExdU1? MkPiNlRp NFGyR5NqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgZ4hmdW:2aHXyZZB6KGS{dXfz MkPxNlQ{OTV3N{i=
UOK257 NFS1T5NCeG:ydH;zbZMhSXO|YYm= Mn7OOY1O MojsN4g> NH;YelFmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NFXWSYkzPDNyNU[wOC=>
ACHN-5968 NGHzSGtCeG:ydH;zbZMhSXO|YYm= M{PBXFVuVQ>? MWCzbC=> Mlyy[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? Mn3iNlQ{ODV4MES=
Huh7 MmjFRZBweHSxc3nzJGF{e2G7 NUfSWWJQOm2P NW\aZXN2OTKq NV\jW|JxTE2VTx?= NX3LRYwxcW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> NVnMfJVTOjR{OUezNFA>
Hep3B M3vqRmFxd3C2b4Ppd{BCe3OjeR?= NGnZOIUzdU1? NIrHOGcyOmh? MUfEUXNQ NWLxSHBLcW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> MViyOFI6PzNyMB?=
RKO MYTGeY5kfGmxbjDBd5NigQ>? MUmycW0> NF7wdVkycA>? Ml;QSG1UVw>? Mn\u[Y5p[W6lZYOgZ4VtdCCmZXH0bEBjgSCpZXzkZY5idXmlaX6= M4nH[|I1OjlzN{e3
HepG2 E47 NG[xZY9HfW6ldHnvckBCe3OjeR?= MnKwNk42dU1? M{nDbFQ5cA>? MXvpcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 NV;lVGFjOjR{N{O3N|g>
LoVo  MmX4RZBweHSxc3nzJGF{e2G7 NUnQVo93PW2P M3OwOVQ5cA>? NGrPNFJmdmijbnPld{BFS0FvaX7keYNm\CCjcH;weI9{cXNw NX71XHV3OjR{MEG4NVI>
WiDr MojZSpVv[3Srb36gRZN{[Xl? NFzzZZgyOG2P M4PRWlFp NYrFcmhJcW6qaXLpeJMhWEOETD3pcoR2[2WmIFzDN{BKUSCneIDy[ZN{cW:w MorBNlQyQTB2OEm=
H1299  NETLVmRHfW6ldHnvckBCe3OjeR?= NX\FeHRoOTCvTR?= MmnWOIg> MmHpbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NWC2WWN6OjRzN{OyNFg>
H460 MkLBSpVv[3Srb36gRZN{[Xl? MXyxNI1O NXLDcXhCPGh? M3zQTYlv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NWfyPVJ2OjRzN{OyNFg>
A549 MnS3SpVv[3Srb36gRZN{[Xl? M{DFe|ExdU1? NXHuOHVOPGh? MVjpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBqenKjZHnheIlwdg>? NXj1eok2OjRzNEK3N|U>
Hep3B MkS3RZBweHSxc3nzJGF{e2G7 MnLwOY1O NE\uPZYzPGh? M1jaUIF1fGWwdXH0[ZMhXE6ILd8xJJBzd3SnY4Tpc44h[WejaX7zeEB{\XK3bTDzeIFzfmG2aX;uMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NEj0XJkzPDB4Nk[5Ny=>
SMMC-7721 MW\BdI9xfG:|aYOgRZN{[Xl? MmP5OY1O M1y1XVI1cA>? NGjl[XRifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> MkjSNlQxPjZ4OUO=
HO8910 Ml3tRZBweHSxc3nzJGF{e2G7 NWC1S49qOTCvTR?= MmO2NVJp MWflcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> NFLVTFQzOzl6M{[xNC=>
MCF7 Mm[wSpVv[3Srb36gRZN{[Xl? MXu1cW0> NIn2[VgzPGh? MX3pcoNz\WG|ZYOgR5VQKGmwZIXj[YQh[2WubDDk[YF1cA>? MWiyN|k3OjZ{OR?=
HONE-1  M3\vSWZ2dmO2aX;uJGF{e2G7 M3OyWlVuVQ>? M2S0dVFp NIjzU5hz\XC{ZYPz[ZMhPnJvbXXkbYF1\WRiUl;TJJBzd2S3Y4Tpc44> NXrzcI9COjN6OUKzOVg>
HeLa MYfGeY5kfGmxbjDBd5NigQ>? NXXxWpl{OTCvTR?= NFTlPYUzcA>? NF7pNYl{fXCycnXzd4V{KEyFMzDJTUBmgHC{ZYPzbZNwdg>? MWKyN|g3PDd|OB?=
HepG2 MYHGeY5kfGmxbjDBd5NigQ>? NGHO[JIyOG2P NXTSW3h[OjSq MVzpcohq[mm2czDzbXRKT0GULTDhcoQhUEKVUz3pcoR2[2WmIHH1eI9xcGGpeR?= NH\kcYQzOzhzN{C0NC=>
SH-SY5Y MYTGeY5kfGmxbjDBd5NigQ>? NWDaWYh6OW2P MWSyOIg> NFzlW3pqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgWG9EWA>? MonmNlM4PDNzNEi=
SH-SY5Y M3:xTmFxd3C2b4Ppd{BCe3OjeR?= NFrkXGM2dU1? NYjvfZZKOWh? NWX4SYRT[WKxbHnzbIV{KGOnbHHzeJJwdCCwZYXyc5Bzd3SnY4TpeoUh\W[oZXP0 NGjXOXQzOzZzOUO5OS=>
PC12  NUT5UWg2TnWwY4Tpc44hSXO|YYm= MmH5NVBuVQ>? MmLHNlRp MX33ZZRmeg>? MWVCpIlvcGmkaYTzJINpgW2xdIL5dJNqdi2uaXvlJJBzd3SnYYPvcYFtKGGldHn2bZR6Ng>? M4fpR|I{PjB|OUe5
OV2008 NHm1ZZZCeG:ydH;zbZMhSXO|YYm= M{LZ[|VuVQ>? Ml3DNlRp MXjjc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? NYKyVllZOjN3OUKyPFE>
A2780 M1HGeWFxd3C2b4Ppd{BCe3OjeR?= NEHBZXk2dU1? MoLBNlRp M1XxfINwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| M{jCZVI{PTl{Mkix
Saos-2  NXK3dGIzSXCxcITvd4l{KEG|c3H5 MlPsNY1O NIrURZg6Pmh? MkS4bY5kemWjc3XzJINmdGxiZHXheIghcW6mdXPl[EBjgSCSQ2i= MVyyN|U3OzF5MR?=
1321N1 MUPDfZRwfG:6aXPpeJkhSXO|YYm= MVm1cW0> M1;DPFI1cA>? MlPvdJJwfGWldIOgZ4VtdCCjZ3HpcpN1KFCFTj3pcoR2[2WmIITvfIlkcXS7 M{mzb|I{PTJ3Mk[1
SH-SY5Y NFX0dYxEgXSxdH;4bYNqfHliQYPzZZk> M{DDVlVuVQ>? MkHENlRp NIrh[21qdmO{ZXHz[ZMhWEOQIITvfIlkcXS7 MY[yN|UzPTJ4NR?=
HT-29  MYnGeY5kfGmxbjDBd5NigQ>? NFHBbmIydU1? NYm0U5dFPDhxOU\o NYC1SopCcW6qaXLpeJMhSU2SSzDpcoR2[2W|IHH1eI9xcGGpaXOgZ4VtdCCmZXH0bC=> NUXaTVRiOjN3MEiyO|I>
OR6 NHnlWYdHfW6ldHnvckBCe3OjeR?= NXnWXlhuOTCvTR?= MVq3Nog> M1ywbpN2eHC{ZYPz[ZMhUEOYIILldIxq[2G2aX;uJIFv\CCob4LtZZRqd25ib3[gZZV1d3CqYXfvd49u\XN? NIDjcnkzOzN7NUi3OS=>
Hela  NVvwZpcxTnWwY4Tpc44hSXO|YYm= MonPOY1O NX20VXRnOjSq NEfJ[5hqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 NF3xPI4zOzN7NU[3PS=>
MCF-7  NGrnOpVHfW6ldHnvckBCe3OjeR?= MoK5OY1O Ml7BNlRp MYHpcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 MkmwNlM{QTV4N{m=
HUVECs NIXGUXRHfW6ldHnvckBCe3OjeR?= M4nTb|NuVQ>? Ml;NNlRp MXnicI9kc3NidHjlJJBzd3SnY4TpeoUh\W[oZXP0JI9nKHKnc4\ldoF1em:uIHL5JIlvcGmkaYTpcoch[XW2b4DoZYd6 NXSxcpRQOjN|NUi5Nlg>
T24 MV3GeY5kfGmxbjDBd5NigQ>? M2rMc|ExdU1? NUHu[mx7OWh? NW\ZRYxiemWmdXPld{B1cGViY3zlZZZi\2Vib3[gUGM{KGGodHXyJIJicWOjbHnuJJRz\WG2bXXueC=> NIrpOpkzOzN3NEC4NC=>
U251MG  MUjGeY5kfGmxbjDBd5NigQ>? MWKzcW0> M{[5Z|Fp NHjYWmd{fXCycnXzd4V{KEyFMz3JTUBxem:2ZXnuJIV5eHKnc4Ppc44> M36yR|I{OzN6NkG4
GTL-16  MnnDRZBweHSxc3nzJGF{e2G7 NH;0fmM2dU1? NULVVmNZOjSq NHLwbpZz\WS3Y3XzJINmdGxidnnhZoltcXS7IHHzJINwdXCjcnXkJJRwKGOnbHzzJJRz\WG2ZXSge4l1cCCPRWSgbY5pcWKrdH;ydy=> NWrlPWZ7OjN|MUO0PVA>
T-47D MWPGeY5kfGmxbjDBd5NigQ>? NF7ib5UyOG2P M3y0UlJp NIXhVmlqdmirYnn0d{BifXSxcHjh[5kheHKxY3Xzd{BidmRiaX7jdoVie2W|IILhdIFugWOrbjDpcoR2[2WmIHHwc5B1d3Orcx?= NX2yOXl1OjN|MECwNlY>
PaCa44 MYrBdI9xfG:|aYOgRZN{[Xl? M324SlIvPW2P NETVdW8ycA>? Mn;XdoVlfWOnc9Mg[4VvcXCrbj3t[YRq[XSnZDDhdI9xfG:|aYO= M3HtPVI{OTJ2MUGy
MDA-MB231 MUfGeY5kfGmxbjDBd5NigQ>? NYHMTGJVPW2P MlTONYg> MnnPbY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> M{\KVFI{ODh6OEWw
HeLa MlnWRZBweHSxc3nzJGF{e2G7 NGrObpgyOG2P Ml3hNog> M{jtOoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDjc{11emWjdH3lcpQhf2m2aDDQSWk> NWe4O49tOjNyMECxN|U>
SK-HEP-1 NWjsN3dxSXCxcITvd4l{KEG|c3H5 NYD4RmZFOTCvTR?= NV22fIh5OWh? Mmn0dJJwfGWldIOgZYdicW6|dDDheZRweGijZ4mgZY5lKGmwZIXj[ZMh[XCxcITvd4l{KGmwIHL1[oFtcW5vdILlZZRm\CClZXzsdy=> NX7pVpJJOjJ6NUi2OFk>
HepG2 M2fnXWFxd3C2b4Ppd{BCe3OjeR?= MnXiN41O MYO1bC=> NWDFO4pWemWmdXPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHFFew>? Ml3ZNlI5OzZ3OUW=
MCF-7  NXLOemR1TnWwY4Tpc44hSXO|YYm= NUPPXWVkOm2P NIf0U2gzPGh? NX7EZ2xYcW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF5CpC=> MmXmNlQ6PzB4N{[=
MDA-MB-231 MUDGeY5kfGmxbjDBd5NigQ>? Mke2No1O NWfGZXN5OjSq M1;HSIlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= MUWyOFk4ODZ5Nh?=
MCF-7  NF7zWmFHfW6ldHnvckBCe3OjeR?= M4XHVlJuVQ>? MXS0PIg> MWPwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> M3vB[lI1QTdyNke2
MDA-MB-231 NFfSeFFHfW6ldHnvckBCe3OjeR?= M1TnSVJuVQ>? NIjjbIU1QGh? MVnwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> NF3rSJIzPDl5ME[3Oi=>
PANC-1  NIHiVYZCeG:ydH;zbZMhSXO|YYm= M{PSSVFuVQ>? NEDNeo01QGh? NIPJTY1FVVOR M{PveoVvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NInQXnczPDh2MkG1PC=>
MDA-MB 231 MYXBdI9xfG:|aYOgRZN{[Xl? NEXNWWI2dU1? NV7lWoVMOC53aB?= NEDtc5Fud2S3bHH0[ZMhXG:lb33pcuKvyqCrbnT1Z4VlKGGyb4D0c5Nqew>? MlKzNlQ5OzB5OEG=
Microglia  NVPuW|VvSXCxcITvd4l{KEG|c3H5 NHyxbYU2dU1? M1LaSlI1cA>? NWXjOIVu\GWlcnXhd4V{KGi7cH;4bYEucW6mdXPl[EBk\WyuIHTlZZRp MWKyOFgyQDZyMR?=
A2780cp NYPESmh1SXCxcITvd4l{KEG|c3H5 M{j1dVIvPW2P NFjjSokycA>? M2LyU4RlUDKR M4LTSYVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MYmyOFgyPzl2Nh?=
HepG2 M2W4c2Z2dmO2aX;uJGF{e2G7 MXu1cW0> M3W3SFRp M{DKdYlv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?= MUeyOFcyOzV6Nx?=
U2OS NYrQWIltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjh[28yOG2P NVKze4xmOjSq MkfGbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIHnu[JVk\WRiYomgSI95 MVyyOFY{QTBzMx?=
HCT116 NX\UdplxSXCxcITvd4l{KEG|c3H5 MkjQOY1O MlfKNlRp MUnEUXNQ M3jFRoVvcGGwY3XzJIFxcWenbnnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NV;MO4xzOjR4Mk[1NlI>
MCF-7  MmLnRZBweHSxc3nzJGF{e2G7 MUmwMlFuVQ>? MXO2bC=> NH3ZVoNmdmijbnPld{B{cXK2aX7vcE1qdmS3Y3XkJIFxd3C2b4Ppdy=> MUKyNlc2OTl6OR?=
PC-3  NX\a[YY1SXCxcITvd4l{KEG|c3H5 NX7zN5pZOm2P NXrCXVJOOmh? MWTpcoNz\WG|ZYOgU3JKNWmwZIXj[YQh[2WubDDk[YF1cA>? MXuyNlc1PTV6MB?=
U251  NXTSRldWSXCxcITvd4l{KEG|c3H5 Mk[2OY1O M37DTVI1cA>? M3Gxbolv[3KnYYPld{BUOS2rbnT1Z4VlKGOnbHyg[IVifGh? M3\aeFIzPTd7N{i4
HeLa  NG\FcmRCeG:ydH;zbZMhSXO|YYm= NHnyWoU2dU1? NGDySWIzPGh? NE\6XGVqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> Mk\lNlI2PDVzMki=
A549 MVTGeY5kfGmxbjDBd5NigQ>? MmHZNE4ydU1? M2OyN|I1cA>? MX\zeZBxemW|c3XzJHNWOTF{N{StbY5lfWOnZDDj[YxtKGSnYYTo NIftXnYzOjR4Nkm2NC=>
pDCs MULGeY5kfGmxbjDBd5NigQ>? NIrtUnQyOG2P NXnNbHBkOC53aB?= MUZCpJJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDJSm4u|rFiYomgd5NTVkF2MB?= Mmj0NlI{QTZ3OUm=
BGC-823 MXfGeY5kfGmxbjDBd5NigQ>? MU[1cW0> M3[1flJp NXTRRXJ1cW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? NHXYPXczOjN{MkG1Ni=>
U937 NYTofYhITnWwY4Tpc44hSXO|YYm= MWSycW0> M1O0XlEzcA>? M4rjPYRm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? MXiyNlE2PTF3MB?=
Marc-145 NYrVUoR5TnWwY4Tpc44hSXO|YYm= M3P4W|VuVQ>? M3XTPFEzNzJ2L{O2bC=> Mn\4doVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w NITXW|gzOjFzOUmwNC=>
HBx  NV;OWpFTSXCxcITvd4l{KEG|c3H5 M17uPVExdU1? Ml;HOFhp M17PVGROW09? M{i4TIlv[3KnYYPld{Bk\WyuIHTlZZRp NV3DWGhxOjJyMkCwO|g>
MCF-7 MkXGSpVv[3Srb36gRZN{[Xl? NH;2SVcyOG2P NYDm[2g2PDiq MWPicI9kc3NiYYX0c5Bp[We7IHnu[JVk\WRiYomgZo9zfGW8b33pZi=> MkDINlE6OzF7M{e=
RMPI8226  M3T4dmZ2dmO2aX;uJGF{e2G7 MVG1cW0> MlvUNYg> NF7JNmF{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w NX7RfZk5OjF7MUW2NlA>
PC12/TetOn NUTKcJB1TnWwY4Tpc44hSXO|YYm= M3[0VVAvOS9zbV2= NH3FOYMyQGh? MUDs[YFleyC2bzFOtU1{gW5qV2SpJIFk[3WvdXzheIlwdixidH;4bYNqfHluIHHu[EBwdGmpb33ldkBnd3KvYYTpc47DqA>? NX[yeZByOjF7ME[2OVk>
HeLa  M3uyc2N6fG:2b4jpZ4l1gSCDc4PhfS=> MU[ycW0> MlvrNlRp NUHFZ|E1cW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv NX;Ue4Z{OjF6N{WzPFU>
Jurkat MkPpSpVv[3Srb36gRZN{[Xl? M2X3fVExdU1? NILWWIMycA>? MnLR[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NVrtVI9GOjF6NkSwN|c>
K562 NEfmZXdHfW6ldHnvckBCe3OjeR?= M2TOVFExdU1? MnjiNYg> MXfk[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gUGM{NUmLIHHu[EB1cGViZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| NWPxTIhbOjF6NkSwN|c>
Nara-H M1rmbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3Hm[|VuVQ>? NUDJTopZPDiq Mnra[Y5p[W6lZYOgeIVue2m{b3zpcZV{dWWmaXH0[YQhe3WycILld5Nqd25ib3[gUoFz[S2KIHPlcIwheHKxbHnm[ZJifGmxbh?= MWiyNVgxPTB|Mx?=
HUVECs M17kTmZ2dmO2aX;uJGF{e2G7 M1XOd|ExdU1? MUKwMlVp MlTD[IVkemWjc3XzJJRp\SCDR1WtRnNCcW6mdXPl[EBifXSxcHjh[5khdGW4ZR?= M1PPfVIyPDZ6NUmy
HepG2 Ml7KRZBweHSxc3nzJGF{e2G7 M3KzTFJuVQ>? NGPo[5cycA>? MlzF[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NH:5VJIzOTR3M{[5NS=>

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
in solvent
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID