3-Methyladenine (3-MA)

For research use only.

Catalog No.S2767 Synonyms: NSC 66389

591 publications

3-Methyladenine (3-MA) Chemical Structure

CAS No. 5142-23-4

3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.

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Selleck's 3-Methyladenine (3-MA) has been cited by 591 publications

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Biological Activity

Description 3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 NGXiNVhHfW6ldHnvckBCe3OjeR?= NXz3WmxnOTCvTR?= MUKxbC=> MoXz[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= M3zn[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOE[0NFM4Lz5{MUi2OFA{PzxxYU6=
Jurkat M1fpR2Z2dmO2aX;uJGF{e2G7 NEPZSVQyOG2P MUSxbC=> M4DzbIRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= NIj3bYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi2OFA{Pyd-MkG4OlQxOzd:L3G+
HeLa  M{X6V2N6fG:2b4jpZ4l1gSCDc4PhfS=> MV2ycW0> NU[yNoFzOjSq M3m3TYlvcGmkaYTld{B1cGViY4n0c5RwgGmlaYT5JI9nKHOrbHnibY5qdiC2bzDI[WxiKGOnbHzzMi=> M{\sSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEe1N|g2Lz5{MUi3OVM5PTxxYU6=
PC12/TetOn NGD3fHJHfW6ldHnvckBCe3OjeR?= NHzJTYcxNjFxMX3N NULjeIFuOTiq NX;INncydGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= NHTwfJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwOlY2QSd-MkG5NFY3PTl:L3G+
RMPI8226  M2\j[mZ2dmO2aX;uJGF{e2G7 NEHzS5g2dU1? NGjHcpkycA>? NHrIc2t{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w M2Lx[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUG1OlIxLz5{MUmxOVYzODxxYU6=
MCF-7 NVGyUnM6TnWwY4Tpc44hSXO|YYm= MnTONVBuVQ>? MoDKOFhp M4jUdoJtd2OtczDheZRweGijZ4mgbY5lfWOnZDDifUBjd3K2ZYrvcYlj NF;TTXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmzNVk{Pyd-MkG5N|E6Ozd:L3G+
HBx  Ml3aRZBweHSxc3nzJGF{e2G7 MnXSNVBuVQ>? M37ZVFQ5cA>? NX7nbGhiTE2VTx?= M2j5dolv[3KnYYPld{Bk\WyuIHTlZZRp MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{MEC3PEc,OjJyMkCwO|g9N2F-
Marc-145 MYXGeY5kfGmxbjDBd5NigQ>? NHfuWpY2dU1? NUmzW4pxOTJxMkSvN|Zp MonkdoVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzOUmwNEc,OjJzMUm5NFA9N2F-
U937 M1;JcmZ2dmO2aX;uJGF{e2G7 M4jVbVJuVQ>? M3jKe|EzcA>? NI\LXG5l\WO{ZXHz[ZMhfGinIHH1eI9xcGGpeTDyZZRqd8Li M3PIN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUW1NVUxLz5{MkG1OVE2ODxxYU6=
BGC-823 MWLGeY5kfGmxbjDBd5NigQ>? MlfpOY1O MUWybC=> NVH1Vo5VcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MkG1Nkc,OjJ|MkKxOVI9N2F-
A549 M1e3emZ2dmO2aX;uJGF{e2G7 MnO3NE4ydU1? M3j4e|I1cA>? MYTzeZBxemW|c3XzJHNWOTF{N{StbY5lfWOnZDDj[YxtKGSnYYTo MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4Nkm2NEc,OjJ2Nk[5OlA9N2F-
pDCs MWfGeY5kfGmxbjDBd5NigQ>? NHLSSJkyOG2P NIHWd4oxNjWq MnzmxsBz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gTWZPNc7zIHL5JJN{Wk6DNEC= M4LHTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{m2OVk6Lz5{MkO5OlU6QTxxYU6=
HeLa  MXjBdI9xfG:|aYOgRZN{[Xl? M3PJfVVuVQ>? M{S0VlI1cA>? NFPMcIFqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> NGH0bXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0OVEzQCd-MkK1OFUyOjh:L3G+
U251  MlywRZBweHSxc3nzJGF{e2G7 MUe1cW0> M{C3e|I1cA>? MW\pcoNz\WG|ZYOgV|EucW6mdXPl[EBk\WyuIHTlZZRp M1fPbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUe5O|g5Lz5{MkW3PVc5QDxxYU6=
MCF-7  MWfBdI9xfG:|aYOgRZN{[Xl? NVn4O5hiOC5zbV2= NVHISZRvPmh? M3;zcIVvcGGwY3XzJJNqenSrbn;sMYlv\HWlZXSgZZBweHSxc3nz MmnYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NUG5PFkoRjJ{N{WxPVg6RC:jPh?=
PC-3  Mn7ZRZBweHSxc3nzJGF{e2G7 NIft[5gzdU1? M1PTZlJp MoLKbY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? MlPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEW1PFAoRjJ{N{S1OVgxRC:jPh?=
HCT116 MlHURZBweHSxc3nzJGF{e2G7 NXP1fWZxPW2P MkfoNlRp M1;4fWROW09? MknD[Y5p[W6lZYOgZZBq\2WwaX6tbY5lfWOnZDDj[YxtKGSnYYTo MlHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[1NlIoRjJ2NkK2OVIzRC:jPh?=
U2OS NXfBNnRTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;nNVBuVQ>? NIDpNo4zPGh? NGG4N5FqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gbY5lfWOnZDDifUBFd3h? NWrGXo9LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOTNpPkK0OlM6ODF|PD;hQi=>
A2780cp NFrYV5lCeG:ydH;zbZMhSXO|YYm= MlqxNk42dU1? MXqxbC=> M4njO4RlUDKR NI\5UYJmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzN{m0Okc,OjR6MUe5OFY9N2F-
HepG2 MX3GeY5kfGmxbjDBd5NigQ>? MWC1cW0> M1XBNlRp MlnGbY5kemWjc3XzJINmdGy3bHHyJIxmfmWuczDv[kBJVMLi NUfwW5c5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVM2QDdpPkK0O|E{PTh5PD;hQi=>
Microglia  MYfBdI9xfG:|aYOgRZN{[Xl? M1fn[|VuVQ>? NV;oToFsOjSq M1frVoRm[3KnYYPld{BpgXCxeHnhMYlv\HWlZXSgZ4VtdCCmZXH0bC=> M4rke|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG4OlAyLz5{NEixPFYxOTxxYU6=
MDA-MB 231 Mnv3RZBweHSxc3nzJGF{e2G7 NVvNVYRHPW2P M{OzclAvPWh? M2G5U41w\HWuYYTld{BVd2OxbXnuxs7DqGmwZIXj[YQh[XCxcITvd4l{ NWXyXGpURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4N|A4QDFpPkK0PFMxPzhzPD;hQi=>
PANC-1  M4XBUmFxd3C2b4Ppd{BCe3OjeR?= NYXQNpBCOW2P MXm0PIg> M3POOmROW09? MlXn[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> Ml;ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEKxOVgoRjJ2OESyNVU5RC:jPh?=
MDA-MB-231 Ml3QSpVv[3Srb36gRZN{[Xl? NFu4[HYzdU1? M2fM[FQ5cA>? M2radJBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl5ME[3Okc,OjR7N{C2O|Y9N2F-
MDA-MB-231 NFnFRWVHfW6ldHnvckBCe3OjeR?= MmDUNo1O NYC5OmlLOjSq MVnpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi MonNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7N{C2O|YoRjJ2OUewOlc3RC:jPh?=
MCF-7  NVLtUYg4TnWwY4Tpc44hSXO|YYm= MXuycW0> NIToPVM1QGh? NEX4d5Fxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? M33CUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
MCF-7  NV7aPHhVTnWwY4Tpc44hSXO|YYm= Ml3INo1O NXSxbGQ1OjSq M1LjW4lvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= NWfz[|NRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
HepG2 MlXpRZBweHSxc3nzJGF{e2G7 NYDmOWxuO22P NYS0SI5vPWh? MoHSdoVlfWOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGHEdy=> MlzsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{[1PVUoRjJ{OEO2OVk2RC:jPh?=
HeLa MWrBdI9xfG:|aYOgRZN{[Xl? MnPFNVBuVQ>? NY\NU4x4Omh? MV;k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSvZX70JJdqfGhiUFXJ MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzByMEGzOUc,OjNyMECxN|U9N2F-
SK-HEP-1 M1noXWFxd3C2b4Ppd{BCe3OjeR?= M3mx[VExdU1? NXOwUYhGOWh? NWG5PGF1eHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? NIjZVnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1PFY1QSd-MkK4OVg3PDl:L3G+
MDA-MB231 MWDGeY5kfGmxbjDBd5NigQ>? M4LHbFVuVQ>? NULK[|ViOWh? NHOxOJJqdmO{ZXHz[ZMhemW|dnXyZZRzd2xvbXXkbYF1\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRp M{TpNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEi4PFUxLz5{M{C4PFg2ODxxYU6=
PaCa44 MX3BdI9xfG:|aYOgRZN{[Xl? Mn74Nk42dU1? MknuNYg> Mn:zdoVlfWOnc9Mg[4VvcXCrbj3t[YRq[XSnZDDhdI9xfG:|aYO= NWrpb5RXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxNlQyOTJpPkKzNVI1OTF{PD;hQi=>
T-47D M{fid2Z2dmO2aX;uJGF{e2G7 NELEemkyOG2P MYKybC=> NWnsfVhEcW6qaXLpeJMh[XW2b4DoZYd6KHC{b3Pld5Mh[W6mIHnuZ5Jm[XOnczDyZZBidXmlaX6gbY5lfWOnZDDhdI9xfG:|aYO= NVTSeoQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFAxOjZpPkKzN|AxODJ4PD;hQi=>
GTL-16  M2PwTmFxd3C2b4Ppd{BCe3OjeR?= MnTrOY1O NWm2ZYgxOjSq NIPHO4Rz\WS3Y3XzJINmdGxidnnhZoltcXS7IHHzJINwdXCjcnXkJJRwKGOnbHzzJJRz\WG2ZXSge4l1cCCPRWSgbY5pcWKrdH;ydy=> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzM{S5NEc,OjN|MUO0PVA9N2F-
U251MG  MYnGeY5kfGmxbjDBd5NigQ>? MojXN41O MYixbC=> NV;wOJFue3WycILld5NmeyCOQ{OtTWkheHKxdHXpckBmgHC{ZYPzbY9v M4jO[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{O4OlE5Lz5{M{OzPFYyQDxxYU6=
T24 NXH6fWx[TnWwY4Tpc44hSXO|YYm= NYfOS|d3OTCvTR?= NIDG[oQycA>? M1HWUpJm\HWlZYOgeIhmKGOuZXH2ZYdmKG:oIFzDN{Bi\nSncjDiZYlk[WyrbjD0doVifG2nboS= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3NEC4NEc,OjN|NUSwPFA9N2F-
HUVECs MUjGeY5kfGmxbjDBd5NigQ>? M{O2VlNuVQ>? NXzhbYF7OjSq MmqwZoxw[2u|IITo[UBxem:2ZXP0bZZmKGWoZnXjeEBw\iC{ZYP2[ZJifHKxbDDifUBqdmirYnn0bY5oKGG3dH;wbIFogQ>? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3OEmyPEc,OjN|NUi5Nlg9N2F-
MCF-7  MonpSpVv[3Srb36gRZN{[Xl? M1vic|VuVQ>? NYficYtLOjSq M1jnd4lvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> NIXE[5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVY4QSd-MkOzPVU3Pzl:L3G+
Hela  M2Pt[WZ2dmO2aX;uJGF{e2G7 Ml7rOY1O M2PiR|I1cA>? M2LyUolvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> M{[4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{m1Olc6Lz5{M{O5OVY4QTxxYU6=
OR6 NInQc3NHfW6ldHnvckBCe3OjeR?= MljVNVBuVQ>? NXvFZoZZPzKq NYXafoJ1e3WycILld5NmeyCKQ2[gdoVxdGmlYYTpc44h[W6mIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NF;ncZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVg4PSd-MkOzPVU5PzV:L3G+
HT-29  MnLzSpVv[3Srb36gRZN{[Xl? M2DLdFFuVQ>? MWm0PE86Pmh? MYDpcohq[mm2czDBUXBMKGmwZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSq M1zGRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUC4NlczLz5{M{WwPFI4OjxxYU6=
SH-SY5Y M1\temN6fG:2b4jpZ4l1gSCDc4PhfS=> M1LQN|VuVQ>? MWeyOIg> M2fq[olv[3KnYYPld{BRS05idH;4bYNqfHl? M{jjN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK1NlY2Lz5{M{WyOVI3PTxxYU6=
Saos-2  MnrnRZBweHSxc3nzJGF{e2G7 M3XTcFFuVQ>? M{\OTlk3cA>? M1fMT4lv[3KnYYPld{Bk\WyuIHTlZZRpKGmwZIXj[YQh[nliUFPY Mn74QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NkOxO|EoRjJ|NU[zNVcyRC:jPh?=
1321N1 MnnER5l1d3SxeHnjbZR6KEG|c3H5 NYXBfGFiPW2P NWjnelFXOjSq MlHIdJJwfGWldIOgZ4VtdCCjZ3HpcpN1KFCFTj3pcoR2[2WmIITvfIlkcXS7 MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV{NUK2OUc,OjN3MkWyOlU9N2F-
A2780 NUjONZJKSXCxcITvd4l{KEG|c3H5 M1y0T|VuVQ>? NEHNR28zPGh? NEPQfGFkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= NULoNpljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVIzQDFpPkKzOVkzOjhzPD;hQi=>
OV2008 NHjKeFdCeG:ydH;zbZMhSXO|YYm= NY\oNm5ZPW2P MVSyOIg> NXnlUndZ[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO= NX7ucGNFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVIzQDFpPkKzOVkzOjhzPD;hQi=>
PC12  NYDOcZRDTnWwY4Tpc44hSXO|YYm= M3PLfFExdU1? M4PTeFI1cA>? M4LMbJdifGW{ M{fKR:KhcW6qaXLpeJMh[2i7bX;0dplxe2mwLXzpb4UheHKxdHXhd49u[WxiYXP0bZZqfHlw NInTeZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wN|k4QSd-MkO2NFM6Pzl:L3G+
SH-SY5Y NU\TSYIzSXCxcITvd4l{KEG|c3H5 MUG1cW0> M{Xx[VFp NWLYW282[WKxbHnzbIV{KGOnbHHzeJJwdCCwZYXyc5Bzd3SnY4TpeoUh\W[oZXP0 MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZzOUO5OUc,OjN4MUmzPVU9N2F-
SH-SY5Y MlWwSpVv[3Srb36gRZN{[Xl? NYXUcVVLOW2P M3vtb|I1cA>? NHnOW2xqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgWG9EWA>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2M{G0PEc,OjN5NEOxOFg9N2F-
HepG2 MlXUSpVv[3Srb36gRZN{[Xl? MlrCNVBuVQ>? NETGXXczPGh? M1n2SYlvcGmkaYTzJJNqXEmJQWKtJIFv\CCKQmPTMYlv\HWlZXSgZZV1d3CqYXf5 NGXaXFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ixO|A1OCd-MkO4NVcxPDB:L3G+
HeLa NITkNndHfW6ldHnvckBCe3OjeR?= MXGxNI1O NXvJcnRIOmh? NFr0NWt{fXCycnXzd4V{KEyFMzDJTUBmgHC{ZYPzbZNwdg>? NEP6[nk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i2OFc{QCd-MkO4OlQ4Ozh:L3G+
HONE-1  NFPLeGVHfW6ldHnvckBCe3OjeR?= Mn3ZOY1O M3TmZVFp NVXVOXpmemWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v M{TBOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEmyN|U5Lz5{M{i5NlM2QDxxYU6=
MCF7 MVHGeY5kfGmxbjDBd5NigQ>? M3GzblVuVQ>? MVmyOIg> MU\pcoNz\WG|ZYOgR5VQKGmwZIXj[YQh[2WubDDk[YF1cA>? NFHicY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2NlYzQSd-MkO5OlI3Ojl:L3G+
HO8910 NF3TXZZCeG:ydH;zbZMhSXO|YYm= MWOxNI1O NISyU4kyOmh? MUjlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> NVTYV21MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PFM3OTBpPkKzPVg{PjFyPD;hQi=>
SMMC-7721 MVXBdI9xfG:|aYOgRZN{[Xl? NYfOeWJxPW2P MWKyOIg> M1vESYF1fGWwdXH0[ZMhXE6ILd8xJJBzd3SnY4Tpc44h[WejaX7zeEB{\XK3bTDzeIFzfmG2aX;uMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NIrpTG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2OlY6Oyd-MkSwOlY3QTN:L3G+
Hep3B NV24V|JLSXCxcITvd4l{KEG|c3H5 Ml7GOY1O MUCyOIg> NV71do1Q[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ NV\lNppxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlY3QTNpPkK0NFY3Pjl|PD;hQi=>
H460 MWDGeY5kfGmxbjDBd5NigQ>? M2KyRVExdU1? MmrJOIg> NITmXplqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5M{KwPEc,OjRzN{OyNFg9N2F-
A549 NX3LZY03TnWwY4Tpc44hSXO|YYm= M4TMXVExdU1? MV60bC=> MUDpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBqenKjZHnheIlwdg>? MkfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNEK3N|UoRjJ2MUSyO|M2RC:jPh?=
H1299  NGXYU3RHfW6ldHnvckBCe3OjeR?= MYqxNI1O MljROIg> MoDGbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MnLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{OyNFgoRjJ2MUezNlA5RC:jPh?=
WiDr NInkTIpHfW6ldHnvckBCe3OjeR?= NVTXWm9{OTCvTR?= NX3YbW1qOWh? Mn3zbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v NVLK[nRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxPVA1QDlpPkK0NVkxPDh7PD;hQi=>
LoVo  NIjadoVCeG:ydH;zbZMhSXO|YYm= NH3hZ|Y2dU1? MmPIOFhp M4DRToVvcGGwY3XzJGRESS2rbnT1Z4VlKGGyb4D0c5Nqey5? NFXXdXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwNVgyOid-MkSyNFE5OTJ:L3G+
HepG2 E47 NFv6SpNHfW6ldHnvckBCe3OjeR?= NGT3[4UzNjWvTR?= M1TadVQ5cA>? MUDpcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5M{ezPEc,OjR{N{O3N|g9N2F-
RKO MmHjSpVv[3Srb36gRZN{[Xl? MoTUNo1O M36yR|Fp NFTwfGtFVVOR MWLlcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= NEPDW3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5NVc4Pyd-MkSyPVE4Pzd:L3G+
Hep3B MkfnRZBweHSxc3nzJGF{e2G7 MX:ycW0> NXW5T3BIOTKq NHrXZplFVVOR NHzFS4RqdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> NHLhVXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5O|MxOCd-MkSyPVc{ODB:L3G+
ACHN-5968 M{G1[mFxd3C2b4Ppd{BCe3OjeR?= NE\wW4Q2dU1? NIH1VVg{cA>? MXflcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh M1jjclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{C1OlA1Lz5{NEOwOVYxPDxxYU6=
Huh7 MV\BdI9xfG:|aYOgRZN{[Xl? M2TqS|JuVQ>? MVmxNog> NWj0c2toTE2VTx?= MknJbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= M3PC[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
UOK257 M3zWcGFxd3C2b4Ppd{BCe3OjeR?= NUX3TmZGPW2P M4DPSFNp NV:zW3lx\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= NUD4RXdGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFU3ODRpPkK0N|A2PjB2PD;hQi=>
ECSCs  NHjiWG5CeG:ydH;zbZMhSXO|YYm= NED2enMyOG2P MUG0bC=> M4S3bIRm[3KnYYPld{Bz[XCjbYnjbY4ufHKnYYTl[EBieG:ydH;zbZM> MoTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUmxNFkoRjJ2M{G5NVA6RC:jPh?=
MCF-7  M1TDdmZ2dmO2aX;uJGF{e2G7 NGnmTYIyOG2P M3jSTlI1cA>? M{HXR4lvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDjbIVud3SqZYLhdJkh\HK3Z4O= M3rI[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G1OVc5Lz5{NEOxOVU4QDxxYU6=
SGC-7901  M1PGUWFxd3C2b4Ppd{BCe3OjeR?= M4[ze|JuVQ>? Mm\ONYg> M3;XOIlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| NUe2WIlzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE{PDBpPkK0N|IyOzRyPD;hQi=>
SMMC-7721 MoDFRZBweHSxc3nzJGF{e2G7 NX;jPGE3Om2P M37tUlFp NWLyVpZkcW6lcnXhd4V{KEODLUSgbY5lfWOnZDDhdI9xfG:|aYO= M1Xvb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{KxN|QxLz5{NEOyNVM1ODxxYU6=
T24 NV3M[29XSXCxcITvd4l{KEG|c3H5 MYm1cW0> NVHiNmp3OS53aB?= MW\wc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> NW\rVGpoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFkyPzZpPkK0N|Q6OTd4PD;hQi=>
NTUB1 MX;BdI9xfG:|aYOgRZN{[Xl? M{nzRlVuVQ>? MWqxMlVp Mn;VdI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{ M{\2ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{S5NVc3Lz5{NEO0PVE4PjxxYU6=
MG-63 NXrvN2FKSXCxcITvd4l{KEG|c3H5 NFfOXIsyOG2P MlfnNVJp M2TWPYVvcGGwY3XzJGRRNWmwZIXj[YQh[XCxcITvd4l{ M1;tXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|AyLz5{NEO1PFMxOTxxYU6=
MG-63 NVLWZYw{SXCxcITvd4l{KEG|c3H5 MnOzNE42NzGvTR?= NGjseXc{Omh? M1:2fIVvcGGwY3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= M1jLeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|QzLz5{NEO1PFM1OjxxYU6=
MG-63 MoTISpVv[3Srb36gRZN{[Xl? MWmwMlUwOW2P NVPHe5RnPDiq MlXhbY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OEO0Nkc,OjR|NUizOFI9N2F-
U2OS  M1XYWmZ2dmO2aX;uJGF{e2G7 M335clAvPS9zbV2= MmnmOFhp M13URYlv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NGW4ZXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
HGC-27 MoPrSpVv[3Srb36gRZN{[Xl? NITYOogyOG2P MmO0NYg> MV;pcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgcI9{eyCkeTDSRWQxODFib4KgUWsuOjJyNh?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDRzNkO0PUc,OjR2MU[zOFk9N2F-
HCT116  NXXIXJRXSXCxcITvd4l{KEG|c3H5 Mo\FOY1O M1nZNFI1cA>? MWTlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? NE\hSmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[yOlUzOid-MkS2NlY2OjJ:L3G+
A549 NHHWOGtCeG:ydH;zbZMhSXO|YYm= MWixNI1O NYXMenQ2PDiq M331XIFk[2WuZYLheIV{KHSqZTDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBRXFh? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NkeyNkc,OjR4Mk[3NlI9N2F-
Saos-2 NIPBboVCeG:ydH;zbZMhSXO|YYm= NH\BUpEyOG2P MYmyOIg> MYHpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OUCxN{c,OjR4M{mwNVM9N2F-
U2OS MWDBdI9xfG:|aYOgRZN{[Xl? MXexNI1O MViyOIg> MlPzbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJJRp\SCXMl;TJINmdGy|IHnu[JVk\WRiYomgSI95 NH7DTFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zPVAyOyd-MkS2N|kxOTN:L3G+
HepG2 NXfVenlXTnWwY4Tpc44hSXO|YYm= M3\V[lVuVQ>? NETFcIQ1cA>? M2THOIlv[3KnYYPld{BJVCC{ZXzlZZNm NUD5WnpNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVM2QDdpPkK0O|E{PTh5PD;hQi=>
A549 MWfBdI9xfG:|aYOgRZN{[Xl? Ml2xOY1O MYC0PIg> MkGw[IVkemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGxiZHXheIgh[W6mIHX4dJJme3Orb36gcIV3\Wy|IH;mJINie3Cjc3WtN{whSmWlbHnuMVEh[W6mIFzDN{1KUQ>? MmTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5ME[zNFMoRjJ2N{C2N|A{RC:jPh?=
A2780cp MYLBdI9xfG:|aYOgRZN{[Xl? NILKdWczNjWvTR?= M1radFFp NEPrdIFl\Eh{Tx?= M3OyV4VvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NXXneFRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVc6PDZpPkK0PFE4QTR4PD;hQi=>
Microglia  MWHBdI9xfG:|aYOgRZN{[Xl? M1[3blVuVQ>? NGLzeZMzPGh? MVXk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MnL1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUi2NFEoRjJ2OEG4OlAyRC:jPh?=
HT-29  M{HLUGFxd3C2b4Ppd{BCe3OjeR?= MoHpNY1O NYX2SFBEPDiq NFjr[FNFVVOR MmHX[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> M3fhRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OESyNVU5Lz5{NEi0NlE2QDxxYU6=
MDR NEm1d3lCeG:ydH;zbZMhSXO|YYm= MojtNVBuVQ>? MXG2bC=> MXPzeJJmdme2aHXud{B1cGVicH;3[ZIhd2ZiYX70bYNidmOncjDkdpVoew>? M1HreFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEG5O|AyLz5{NUCxPVcxOTxxYU6=
H157 NEfkZ4FHfW6ldHnvckBCe3OjeR?= NUTaPHNXPW2P MkLtNog> MXjzeZBxemW|c3XzJHNRSyCrbnT1Z4VlKGGlY4XteYxifGmxbjDv[kBNSzNvSVm= NHvDPVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK4OVYzQCd-MkWyPFU3Ojh:L3G+
A549  MmmwSpVv[3Srb36gRZN{[Xl? MXW1cW0> M4PsRVJp NVXJSIhte3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK MmDjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{OEW2NlgoRjJ3Mki1OlI5RC:jPh?=
A2780cp  MlGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\zNY1O MVOxbC=> NHzjbFdqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MmfJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MkK2PVQoRjJ3M{KyOlk1RC:jPh?=
NBL-W-S  NHvDXmxCeG:ydH;zbZMhSXO|YYm= NVXoS3puOW2P M1Lr[FZp NVq5e4JkcW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhT0GQVD22NS=> M3LPc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{KzNlIzLz5{NUOyN|IzOjxxYU6=
NBL-W-S  MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PEXlFuVQ>? NETuRmE3cA>? Mnnl[Y5p[W6lZYOgS2FPXC14MTD0c5hq[2m2eR?= NVnJPWhGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNlMzOjJpPkK1N|I{OjJ{PD;hQi=>
A549  NHy1VFFCeG:ydH;zbZMhSXO|YYm= NVLCc4xRPW2P MlzLNog> MXvEUXNQ MnPtbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq NUWwW5NQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVY2PjFpPkK1O|E3PTZzPD;hQi=>
95D NGK4T3ZCeG:ydH;zbZMhSXO|YYm= NEjlcHk2dU1? NWLDRopvOmh? NFzHd5pFVVOR M3ftTIlvcGmkaYTzJGJFVUNvaX7keYNm\CCjcH;weI91cWNiY3XscEBl\WG2aB?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNkW2NUc,OjV5MU[1OlE9N2F-
A549  NGfYSmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnqVFU{dU1? MVKybC=> NYW2SZgzTE2VTx?= Ml[3doVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNkW2NUc,OjV5MU[1OlE9N2F-
95D NITyWZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXS1Z4YyO22P NWXUVWQ3Omh? NXz4eXNKTE2VTx?= NHfsU25z\WS3Y3XzJIdzd3e2aDDpcohq[mm2b4L5JIVn\mWldDDv[kBDTE2F MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdzNkW2NUc,OjV5MU[1OlE9N2F-
Nara-H MljyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDEW|hOPW2P Mnm1OFhp MVPlcohidmOnczD0[Y1{cXKxbHnteZNu\WSrYYTl[EB{fXCycnXzd4lwdiCxZjDOZZJiNUhiY3XscEBxem:uaX\ldoF1cW:w NUOxToNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4NFUxOzNpPkKxPFA2ODN|PD;hQi=>
HUVECs NYfod3o4TnWwY4Tpc44hSXO|YYm= MkPuNVBuVQ>? NVfEbWZJOC53aB?= NHXufGdl\WO{ZXHz[ZMhfGinIFHHSU1DW0GrbnT1Z4VlKGG3dH;wbIFogSCuZY\l M3[zPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNE[4OVkzLz5{MUS2PFU6OjxxYU6=
HepG2 Mm\WRZBweHSxc3nzJGF{e2G7 MmjuNo1O MlqzNYg> MlrG[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NIrHeIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1N|Y6OSd-MkG0OVM3QTF:L3G+
U-2 OS Mn7kdWhVWyCjc4PhfS=> M33tfZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCXLUKgU3Mh[2WubIO= MmP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 NH7GXoZyUFSVIHHzd4F6 NXPTSWlkeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= NYPobpU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
DAOY MXTxTHRUKGG|c3H5 MX7xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? MlXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 MW\xTHRUKGG|c3H5 MkT3dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= M3juPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 M36x[JFJXFNiYYPzZZk> M3flRpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= NWLXVHZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD MlrzdWhVWyCjc4PhfS=> MlXSdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ NVPoUHhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH NHrD[|RyUFSVIHHzd4F6 NVu3Vmw6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 M2nvNpFJXFNiYYPzZZk> NVz2N2hPeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) Ml7jdWhVWyCjc4PhfS=> MnrRdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| M4DqbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MlvRdWhVWyCjc4PhfS=> MlnIdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= M1\MWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 NV;0Z2xjeUiWUzDhd5NigQ>? MkTtdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= NIr0NXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 MXLxTHRUKGG|c3H5 NV;ySXhCeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoOFEh[2WubIO= MmXvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh30 NWX3fldDeUiWUzDhd5NigQ>? M3TWOpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEOwJINmdGy| M1rFdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NUPHelh3eUiWUzDhd5NigQ>? M2HEXpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz M4izRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MmTLdWhVWyCjc4PhfS=> MUHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MkW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 MWPxTHRUKGG|c3H5 NHnRZoZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=> MnnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 NH\r[FByUFSVIHHzd4F6 MkO5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| M1roSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 MVfxTHRUKGG|c3H5 NYfofnBNeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoNVgh[2WubIO= MoTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65; 

PubMed: 29296191     


Western blotting analysed expression of α-SMA, TGF-β, LC-3B, Beclin-1, NF-κB and NF-κBp65 in hepatic stellate cells.

caspase-3 / caspase-9 / PARP; 

PubMed: 25891159     


EC9706 cell lysates were prepared and subjected to immunoblotting with antibodies against caspase-3, caspase-9 and PARP. The expression ratios in each experiment were determined densitometrically. The results are the averages of triplicate samples. Error bars represent the standard error.*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; PI, propidum iodide; PARP, poly(ADP ribose) polymerase.

VEGF; 

PubMed: 25891159     


Effects of autophagy inhibition on the protein expression levels of VEGF. Western blot analysis demonstrated that VEGF protein expression levels were decreased when the EC9706 human esophageal squamous cell carcinoma cells were treated with 3-MA. The results are the averages of triplicate samples. Error bars represent the standard error.*P<0.05 vs. IR alone. 3-MA, 3-methyladenine; IR, ionizing radiation; VEGF, vascular endothelial growth factor.

APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2; 

PubMed: 26316755     


Presenilin 1, Nicastrin, and Pen-2 were increased with the 3-MA treatment (*P<0.001) while the other component of the γ-secretase complex (APH-1 and Presenilin 2) has no difference. 3-MA, 3-methyladenine; Con, control; APP, amyloid protein precursor.

29296191 25891159 26316755
Immunofluorescence
LC3 / Hif-α / COX2; 

PubMed: 29039446     


Chemical interventions of autophagy and ischemic-hypoxic injury, assessed using immunochemical double labeling. Compared with the control group, expression levels of HIF-α and COX2 were altered by chemical intervention. (A) Chemical intervention altered the expression of HIF-α and autophagosmes. Following autophagy antagonist (3-MA) intervention, expression of HIF-α was enhanced, compared with that in the OGD group, and was decreased by the autophagy agonist, rapamycin. In the MHY treatment group, expression of HIF-α was similar to that in the 3-MA group. (B) Chemical intervention showed changes in the expression of COX2 and autophagosomes. Changes in the expression of COX2 were similar to changes in HIF-α. Scale bar, 30 µm. OGD, oxygen-glucose deprivation; HIF-α, hypoxia-inducible factor-α; COX2, cyclooxygenase 2; 3-MA, 3-methyladenine; RP, rapamycin; MHY, MHY1485.

29039446
Growth inhibition assay
Cell viability; 

PubMed: 26934124     


KYSE 180 cells were co-treated with EDHB (40 μg/ml) and various concentrations of 3-MA for 24 h (left) or 48 h (right), and cell viability was determined by WST-8 assays.

26934124
In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
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Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
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  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
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  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage 3 years -20°C powder
Synonyms NSC 66389
Smiles CN1C=NC(=N)C2=C1N=CN2

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID