3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

For research use only.

3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.

3-Methyladenine (3-MA) Chemical Structure

CAS No. 5142-23-4

Selleck's 3-Methyladenine (3-MA) has been cited by 657 publications

Purity & Quality Control

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Biological Activity

Description 3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 MWjGeY5kfGmxbjDBd5NigQ>? M2iyfVExdU1? Mnv3NYg> NHHndGVl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz M4TPNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOE[0NFM4Lz5{MUi2OFA{PzxxYU6=
Jurkat M1HBbWZ2dmO2aX;uJGF{e2G7 NVToVFZFOTCvTR?= Mn\RNYg> NWDFXG1H\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEyFMz3JTUBidmRidHjlJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> NFXrfIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi2OFA{Pyd-MkG4OlQxOzd:L3G+
HeLa  M1rKPGN6fG:2b4jpZ4l1gSCDc4PhfS=> NUjvWo9HOm2P NIK0TVQzPGh? NHq1WHlqdmirYnn0[ZMhfGinIHP5eI91d3irY3n0fUBw\iC|aXzpZolvcW5idH:gTIVN[SClZXzsd{4> M2jScVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEe1N|g2Lz5{MUi3OVM5PTxxYU6=
PC12/TetOn M1n3U2Z2dmO2aX;uJGF{e2G7 MXKwMlEwOW2P MlHQNVhp MmmxcIVi\HNidH:g{tEue3mwKGfULUBi[2O3bYXsZZRqd25uIITvfIlkcXS7LDDhcoQhd2yrZ3;t[ZIh\m:{bXH0bY9vyqB? NF;YPFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwOlY2QSd-MkG5NFY3PTl:L3G+
RMPI8226  MnzZSpVv[3Srb36gRZN{[Xl? M4LzblVuVQ>? M1PIR|Fp MYHzeZBxemW|c3XzJJRp\SCuZY\lcEBw\iCjdYTvdIhi\3lidX7k[ZIhdnW2cnnlcpQh\GWybHX0bY9v NVnOPW1xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5NVU3OjBpPkKxPVE2PjJyPD;hQi=>
MCF-7 MWTGeY5kfGmxbjDBd5NigQ>? M{TOTVExdU1? M4PLPFQ5cA>? NUnV[3V3[myxY3vzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGKxcoTlfo9ucWJ? M1fGWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUOxPVM4Lz5{MUmzNVk{PzxxYU6=
HBx  M1mySWFxd3C2b4Ppd{BCe3OjeR?= NXnvV|FIOTCvTR?= NFS2OII1QGh? M33MbWROW09? NUDtV44{cW6lcnXhd4V{KGOnbHyg[IVifGh? NGDx[4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyNFA4QCd-MkKwNlAxPzh:L3G+
Marc-145 MlzoSpVv[3Srb36gRZN{[Xl? MYi1cW0> NU\VTIVkOTJxMkSvN|Zp M{fGPZJm\HWlZYOgeIhmKFCUUmPWJJRqfGW{czDhcoQhfGinIIDyc5RmcW5iZYjwdoV{e2mxbh?= M2r1U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUG5PVAxLz5{MkGxPVkxODxxYU6=
U937 M{i2[mZ2dmO2aX;uJGF{e2G7 M2X5UlJuVQ>? MmGzNVJp NULDdXVG\GWlcnXhd4V{KHSqZTDheZRweGijZ4mgdoF1cW0EoB?= NXjhcJdlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxOVUyPTBpPkKyNVU2OTVyPD;hQi=>
BGC-823 MX\GeY5kfGmxbjDBd5NigQ>? MYS1cW0> MoXLNog> NWX0TJpEcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? NXXteohHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNlIyPTJpPkKyN|IzOTV{PD;hQi=>
A549 MVjGeY5kfGmxbjDBd5NigQ>? NXy2ZVMyOC5zbV2= NYKw[5A4OjSq NHvhdI1{fXCycnXzd4V{KFOXMUGyO|QucW6mdXPl[EBk\WyuIHTlZZRp MoXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2Nk[5OlAoRjJ{NE[2PVYxRC:jPh?=
pDCs MnTRSpVv[3Srb36gRZN{[Xl? MnTxNVBuVQ>? NVfLdZV6OC53aB?= NXu0VZg6yqC{ZXT1Z4V{KHSqZTDpcoR2[3Srb36gc4YhUU[QLd8xJIJ6KHO|Ul7BOFA> NFO5bIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO5OlU6QSd-MkKzPVY2QTl:L3G+
HeLa  MnvHRZBweHSxc3nzJGF{e2G7 MXq1cW0> MU[yOIg> NV7R[ZhIcW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp M4G2TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUS1NVI5Lz5{MkW0OVEzQDxxYU6=
U251  M4nDPGFxd3C2b4Ppd{BCe3OjeR?= MomwOY1O M{TTR|I1cA>? MWjpcoNz\WG|ZYOgV|EucW6mdXPl[EBk\WyuIHTlZZRp M2rXNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUe5O|g5Lz5{MkW3PVc5QDxxYU6=
MCF-7  NWqxdY1KSXCxcITvd4l{KEG|c3H5 Mn;sNE4ydU1? NV60VFA6Pmh? MVnlcohidmOnczDzbZJ1cW6xbD3pcoR2[2WmIHHwc5B1d3Orcx?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3MUm4PUc,OjJ5NUG5PFk9N2F-
PC-3  NUe4TZozSXCxcITvd4l{KEG|c3H5 NG\KUogzdU1? M2jSWFJp NXr5U2E5cW6lcnXhd4V{KE:UST3pcoR2[2WmIHPlcIwh\GWjdHi= M2\N[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S1OVgxLz5{Mke0OVU5ODxxYU6=
HCT116 MnntRZBweHSxc3nzJGF{e2G7 M1jvTFVuVQ>? NISzc|gzPGh? M2rWVGROW09? M3TnNoVvcGGwY3XzJIFxcWenbnnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MkjHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[1NlIoRjJ2NkK2OVIzRC:jPh?=
U2OS M4O0[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWGxNI1O MYqyOIg> M4H4R4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JGRwgA>? NYrVZVNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOTNpPkK0OlM6ODF|PD;hQi=>
A2780cp NWnIPFBCSXCxcITvd4l{KEG|c3H5 M3e3VlIvPW2P MVqxbC=> NEPDWI5l\Eh{Tx?= MmHp[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> Mly3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUe5OFYoRjJ2OEG3PVQ3RC:jPh?=
HepG2 M33zPWZ2dmO2aX;uJGF{e2G7 M{HwPFVuVQ>? MVK0bC=> NYLW[odjcW6lcnXhd4V{KGOnbHz1cIFzKGyndnXsd{Bw\iCKTNMg MnHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUO1PFcoRjJ2N{GzOVg4RC:jPh?=
Microglia  MlW4RZBweHSxc3nzJGF{e2G7 NXjnc2p6PW2P NITnN|gzPGh? MXPk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= NGLMUWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixPFYxOSd-MkS4NVg3ODF:L3G+
MDA-MB 231 NIjOVnpCeG:ydH;zbZMhSXO|YYm= NEjTdIM2dU1? MkXqNE42cA>? MoTlcY9lfWyjdHXzJHRw[2:vaX9CsuKhcW6mdXPl[EBieG:ydH;zbZM> M1Gwb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOwO|gyLz5{NEizNFc5OTxxYU6=
PANC-1  MXzBdI9xfG:|aYOgRZN{[Xl? MnjHNY1O NUP2PGxEPDiq MYLEUXNQ M2rlU4VvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ MmXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEKxOVgoRjJ2OESyNVU5RC:jPh?=
MDA-MB-231 M3H5e2Z2dmO2aX;uJGF{e2G7 NHO0O2kzdU1? MUO0PIg> M4DEdJBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? M1XaXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
MDA-MB-231 NXnxW2NzTnWwY4Tpc44hSXO|YYm= MWmycW0> M4i3U|I1cA>? NIrjTJpqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg MljQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7N{C2O|YoRjJ2OUewOlc3RC:jPh?=
MCF-7  MUXGeY5kfGmxbjDBd5NigQ>? MoHjNo1O MVi0PIg> M{jScpBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? NUnBZ|RtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
MCF-7  NWjLTFRNTnWwY4Tpc44hSXO|YYm= MYCycW0> MW[yOIg> MWrpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NWjEXWNGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
HepG2 M3zpdGFxd3C2b4Ppd{BCe3OjeR?= MYizcW0> MXG1bC=> NV\ZUW9SemWmdXPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHFFew>? NWm5e3RYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|Y2QTVpPkKyPFM3PTl3PD;hQi=>
HeLa NHGyN4dCeG:ydH;zbZMhSXO|YYm= M{XvblExdU1? NVn4fmNxOmh? Mn;o[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2bXXueEB4cXSqIGDFTS=> NUjITmliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFAyOzVpPkKzNFAxOTN3PD;hQi=>
SK-HEP-1 M1vPemFxd3C2b4Ppd{BCe3OjeR?= MXSxNI1O NWW1UXZ6OWh? MWnwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz M{HkNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEW4OlQ6Lz5{Mki1PFY1QTxxYU6=
MDA-MB231 NFPuOGpHfW6ldHnvckBCe3OjeR?= NGL2dWM2dU1? NIS5dpgycA>? M2LMO4lv[3KnYYPld{Bz\XO4ZYLheJJwdC2vZXTpZZRm\CClYYPwZZNmKGGldHn2ZZRqd25iYX7kJINmdGxiZHXheIg> M1HSXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEi4PFUxLz5{M{C4PFg2ODxxYU6=
PaCa44 Mnm2RZBweHSxc3nzJGF{e2G7 MVGyMlVuVQ>? Ml76NYg> NIT5c3dz\WS3Y3XzxsBo\W6rcHnuMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NVvPWIpoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxNlQyOTJpPkKzNVI1OTF{PD;hQi=>
T-47D MkDqSpVv[3Srb36gRZN{[Xl? MYSxNI1O NH;XdGMzcA>? MmPQbY5pcWKrdIOgZZV1d3CqYXf5JJBzd2Onc4OgZY5lKGmwY4LlZZNmeyC{YYDhcZlkcW5iaX7keYNm\CCjcH;weI9{cXN? NH\4SWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OwNFAzPid-MkOzNFAxOjZ:L3G+
GTL-16  NFjIdGZCeG:ydH;zbZMhSXO|YYm= M3noclVuVQ>? NFi4Z44zPGh? MUfy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGG|IHPvcZBiemWmIITvJINmdGy|IITy[YF1\WRid3n0bEBOTVRiaX7obYJqfG:{cx?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzM{S5NEc,OjN|MUO0PVA9N2F-
U251MG  NYO4OGZDTnWwY4Tpc44hSXO|YYm= NUfafGRXO22P MnnYNYg> M4LYT5N2eHC{ZYPz[ZMhVEN|LVnJJJBzd3SnaX6g[ZhxemW|c3nvci=> M1fmOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{O4OlE5Lz5{M{OzPFYyQDxxYU6=
T24 MmjoSpVv[3Srb36gRZN{[Xl? MnrzNVBuVQ>? MoP0NYg> MlLwdoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3NEC4NEc,OjN|NUSwPFA9N2F-
HUVECs NYDHeYFnTnWwY4Tpc44hSXO|YYm= NX;hSmdDO22P MUSyOIg> M3XDWYJtd2OtczD0bIUheHKxdHXjeIl3\SCnZn\lZ5Qhd2ZicnXzeoVz[XS{b3ygZpkhcW6qaXLpeIlv\yCjdYTvdIhi\3l? NYni[m9CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOVg6OjhpPkKzN|U5QTJ6PD;hQi=>
MCF-7  M{P0UGZ2dmO2aX;uJGF{e2G7 M3W2SFVuVQ>? MnviNlRp M2nSfolvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> NHjGSII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVY4QSd-MkOzPVU3Pzl:L3G+
Hela  NWLXXVY{TnWwY4Tpc44hSXO|YYm= M3y2PFVuVQ>? MXqyOIg> M3HOcolvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> NUjrVWRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPVU3PzlpPkKzN|k2Pjd7PD;hQi=>
OR6 NG\CU|BHfW6ldHnvckBCe3OjeR?= MlSwNVBuVQ>? MUO3Nog> M2jGb5N2eHC{ZYPz[ZMhUEOYIILldIxq[2G2aX;uJIFv\CCob4LtZZRqd25ib3[gZZV1d3CqYXfvd49u\XN? M4f5SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{m1PFc2Lz5{M{O5OVg4PTxxYU6=
HT-29  M1XMUmZ2dmO2aX;uJGF{e2G7 NWntdld{OW2P NYG3eHdDPDhxOU\o NGLZXnlqdmirYnn0d{BCVVCNIHnu[JVk\XNiYYX0c5Bp[WerYzDj[YxtKGSnYYTo MmjWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEiyO|IoRjJ|NUC4NlczRC:jPh?=
SH-SY5Y NEjGV4REgXSxdH;4bYNqfHliQYPzZZk> Mmj3OY1O NVvvcmc2OjSq NYfnOZREcW6lcnXhd4V{KFCFTjD0c5hq[2m2eR?= NEf4UZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyOVI3PSd-MkO1NlUzPjV:L3G+
Saos-2  M3[0e2Fxd3C2b4Ppd{BCe3OjeR?= M3LhcFFuVQ>? M4fzT|k3cA>? MlzNbY5kemWjc3XzJINmdGxiZHXheIghcW6mdXPl[EBjgSCSQ2i= NG\EOXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W2N|E4OSd-MkO1OlMyPzF:L3G+
1321N1 NYT4cIRwS3m2b4TvfIlkcXS7IFHzd4F6 MlnBOY1O NV\RbmRoOjSq MX\wdo91\WO2czDj[YxtKGGpYXnud5QhWEOQLXnu[JVk\WRidH;4bYNqfHl? NVvBPWVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlUzPjVpPkKzOVI2OjZ3PD;hQi=>
A2780 M3TqOWFxd3C2b4Ppd{BCe3OjeR?= M{jhdlVuVQ>? MkDXNlRp MXPjc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? NVnOdGxpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVIzQDFpPkKzOVkzOjhzPD;hQi=>
OV2008 MkDMRZBweHSxc3nzJGF{e2G7 NECwWmQ2dU1? NEL5bIMzPGh? MUDjc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? M4PPb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUmyNlgyLz5{M{W5NlI5OTxxYU6=
PC12  M2HqZ2Z2dmO2aX;uJGF{e2G7 M{fMNVExdU1? MUOyOIg> MVv3ZZRmeg>? MYFCpIlvcGmkaYTzJINpgW2xdIL5dJNqdi2uaXvlJJBzd3SnYYPvcYFtKGGldHn2bZR6Ng>? M1\xOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCzPVc6Lz5{M{[wN|k4QTxxYU6=
SH-SY5Y M1fmT2Fxd3C2b4Ppd{BCe3OjeR?= NUnmeHhrPW2P M1vj[FFp M3HYeoFjd2yrc3jld{Bk\Wyjc4Tyc4whdmW3cn;wdo91\WO2aY\lJIVn\mWldB?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZzOUO5OUc,OjN4MUmzPVU9N2F-
SH-SY5Y NYfuTFNnTnWwY4Tpc44hSXO|YYm= MWixcW0> Mo\jNlRp M1KzeYlvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDUU2NR NHP3VYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e0N|E1QCd-MkO3OFMyPDh:L3G+
HepG2 M3LFWmZ2dmO2aX;uJGF{e2G7 NH7rSXQyOG2P NYnaS3cxOjSq MmO3bY5pcWKrdIOgd4lVUUeDUj2gZY5lKEiEU2OtbY5lfWOnZDDheZRweGijZ4m= M37ielxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG3NFQxLz5{M{ixO|A1ODxxYU6=
HeLa MWfGeY5kfGmxbjDBd5NigQ>? MXexNI1O NEDlVYwzcA>? M4\IdJN2eHC{ZYPz[ZMhVEN|IFnJJIV5eHKnc4Ppd49v Ml;3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS3N|goRjJ|OE[0O|M5RC:jPh?=
HONE-1  NGrWXJFHfW6ldHnvckBCe3OjeR?= NGC2c5I2dU1? M3P3NVFp NV3IUIFVemWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v Mn;iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6OUKzOVgoRjJ|OEmyN|U5RC:jPh?=
MCF7 MX;GeY5kfGmxbjDBd5NigQ>? NHH3RpM2dU1? NEnTbmszPGh? NIHsOIlqdmO{ZXHz[ZMhS3WRIHnu[JVk\WRiY3XscEBl\WG2aB?= NEnTWHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2NlYzQSd-MkO5OlI3Ojl:L3G+
HO8910 NWnIR|hzSXCxcITvd4l{KEG|c3H5 Mn7JNVBuVQ>? NH;DT3cyOmh? MXnlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> NV3RUXV[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PFM3OTBpPkKzPVg{PjFyPD;hQi=>
SMMC-7721 MofxRZBweHSxc3nzJGF{e2G7 NYHCZpQzPW2P MoTsNlRp MnzQZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB4Nk[5N{c,OjRyNk[2PVM9N2F-
Hep3B MlLQRZBweHSxc3nzJGF{e2G7 NUHyPGtQPW2P NIj3e2UzPGh? Mk\hZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NFO1eXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2OlY6Oyd-MkSwOlY3QTN:L3G+
H460 MUfGeY5kfGmxbjDBd5NigQ>? MWGxNI1O NIn3UJo1cA>? NGf0WGNqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NGHEW2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3N|IxQCd-MkSxO|MzODh:L3G+
A549 NIe3SZNHfW6ldHnvckBCe3OjeR?= MVexNI1O MmPROIg> NES2dlVqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCrcoLh[IlifGmxbh?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2MkezOUc,OjRzNEK3N|U9N2F-
H1299  Mo\NSpVv[3Srb36gRZN{[Xl? NGPhXowyOG2P NIe1NYI1cA>? NELOV2lqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M3zmPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUezNlA5Lz5{NEG3N|IxQDxxYU6=
WiDr NU\KfWFlTnWwY4Tpc44hSXO|YYm= NVzXZ2pIOTCvTR?= MlOzNYg> Mn3ibY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v MkLFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOUC0PFkoRjJ2MUmwOFg6RC:jPh?=
LoVo  M1vsZWFxd3C2b4Ppd{BCe3OjeR?= MmW1OY1O M2DtZlQ5cA>? MWPlcohidmOnczDER2EucW6mdXPl[EBieG:ydH;zbZMv MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJyMUixNkc,OjR{MEG4NVI9N2F-
HepG2 E47 NXjiUXp{TnWwY4Tpc44hSXO|YYm= M4rQTVIvPW2P NX7OPW9EPDiq MU\pcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 M{DtRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkezO|M5Lz5{NEK3N|c{QDxxYU6=
RKO NIPjfYtHfW6ldHnvckBCe3OjeR?= NWfiOYFLOm2P MUSxbC=> M1rrN2ROW09? MnnB[Y5p[W6lZYOgZ4VtdCCmZXH0bEBjgSCpZXzkZY5idXmlaX6= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7MUe3O{c,OjR{OUG3O|c9N2F-
Hep3B M3n1VGFxd3C2b4Ppd{BCe3OjeR?= NHzaWJQzdU1? MYixNog> MYfEUXNQ MkXLbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= M4rKcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
ACHN-5968 MXfBdI9xfG:|aYOgRZN{[Xl? MoXmOY1O MWKzbC=> M3vZU4VvcGGwY3XzJJBi[2yrdHH4[YwudWWmaXH0[YQh[XCxcITvd4l{yqB? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNyNU[wOEc,OjR|MEW2NFQ9N2F-
Huh7 M2HoWGFxd3C2b4Ppd{BCe3OjeR?= NHfLR5UzdU1? M3LmcVEzcA>? NGDV[nVFVVOR NGLwZ3lqdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MkG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N|AxRC:jPh?=
UOK257 M{LuR2Fxd3C2b4Ppd{BCe3OjeR?= MmX5OY1O NFLmNlc{cA>? NHLJXoZmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NF2ycFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwOVYxPCd-MkSzNFU3ODR:L3G+
ECSCs  M1fOemFxd3C2b4Ppd{BCe3OjeR?= MkHjNVBuVQ>? M{fxblRp NUDx[Gps\GWlcnXhd4V{KHKjcHHtfYNqdi22cnXheIVlKGGyb4D0c5Nqew>? MkXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUmxNFkoRjJ2M{G5NVA6RC:jPh?=
MCF-7  MorWSpVv[3Srb36gRZN{[Xl? NYnPcXR{OTCvTR?= M1\tSVI1cA>? M3Xqd4lvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDjbIVud3SqZYLhdJkh\HK3Z4O= NFPFRWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOxOVU4QCd-MkSzNVU2Pzh:L3G+
SGC-7901  M{TiVWFxd3C2b4Ppd{BCe3OjeR?= NIjub|IzdU1? M3rwclFp NH3scm9qdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= M{jxN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{KxN|QxLz5{NEOyNVM1ODxxYU6=
SMMC-7721 MXTBdI9xfG:|aYOgRZN{[Xl? NXjTNG9TOm2P NXPWZ2tCOWh? NGDp[ZhqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{MUO0NEc,OjR|MkGzOFA9N2F-
T24 MVLBdI9xfG:|aYOgRZN{[Xl? NWjCV2hXPW2P MWOxMlVp NGm0ZZpxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? NYHDOnl5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFkyPzZpPkK0N|Q6OTd4PD;hQi=>
NTUB1 MUDBdI9xfG:|aYOgRZN{[Xl? NUK3d4dkPW2P MonWNU42cA>? M373SZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? NXTmWZF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFkyPzZpPkK0N|Q6OTd4PD;hQi=>
MG-63 M4rwd2Fxd3C2b4Ppd{BCe3OjeR?= NHfWeJMyOG2P NV6wV3gxOTKq NF3nOVZmdmijbnPld{BFWC2rbnT1Z4VlKGGyb4D0c5Nqew>? NGKxfVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFMxOSd-MkSzOVg{ODF:L3G+
MG-63 MWjBdI9xfG:|aYOgRZN{[Xl? NX;ne2N[OC53L{HtUS=> NWO4W4tJOzKq MUHlcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| NHXDOJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
MG-63 M1myXGZ2dmO2aX;uJGF{e2G7 M3vEZVAvPS9zbV2= NVfqVVREPDiq MVnpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> Mnv4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUizOFIoRjJ2M{W4N|QzRC:jPh?=
U2OS  NGLwXmFHfW6ldHnvckBCe3OjeR?= NHS5ZVExNjVxMX3N MX[0PIg> NES4c41qdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? M2Dke|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|QzLz5{NEO1PFM1OjxxYU6=
HGC-27 NIOwfWRHfW6ldHnvckBCe3OjeR?= MnzjNVBuVQ>? MUGxbC=> Ml3ZbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JIxwe3NiYomgVmFFODBzIH;yJG1MNTJ{ME[= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDRzNkO0PUc,OjR2MU[zOFk9N2F-
HCT116  MYTBdI9xfG:|aYOgRZN{[Xl? NUO0SFQyPW2P MYKyOIg> NFzPZXBmdmijbnPld{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgZZBq\2WwaX6= MmnRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[1NlIoRjJ2NkK2OVIzRC:jPh?=
A549 NWO3cndCSXCxcITvd4l{KEG|c3H5 MV[xNI1O M3uzcFQ5cA>? MYLhZ4NmdGW{YYTld{B1cGVicnXkeYN1cW:wIH;mJINmdGxidnnhZoltcXS7IHnu[JVk\WRiYomgVHRZ MnXkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Mk[3NlIoRjJ2NkK2O|IzRC:jPh?=
Saos-2 M4jGdWFxd3C2b4Ppd{BCe3OjeR?= MlLYNVBuVQ>? Ml3nNlRp MlvYbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJJRp\SCXMl;TJINmdGy|IHnu[JVk\WRiYomgSI95 MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OUCxN{c,OjR4M{mwNVM9N2F-
U2OS NH;JOHpCeG:ydH;zbZMhSXO|YYm= NFTlcnoyOG2P Mn31NlRp NYGxW|JbcW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 M2fJU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkO5NFE{Lz5{NE[zPVAyOzxxYU6=
HepG2 NWD6dIRrTnWwY4Tpc44hSXO|YYm= M3Lj[VVuVQ>? NIL4W5I1cA>? NHXucGxqdmO{ZXHz[ZMhUExicnXs[YF{\Q>? NXvPTWhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVM2QDdpPkK0O|E{PTh5PD;hQi=>
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HT-29  NHXrd4VCeG:ydH;zbZMhSXO|YYm= M4TkOlFuVQ>? NGDtNm01QGh? MYnEUXNQ MUDlcohidmOnczDic5J1\XqxbXniMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2MkG1PEc,OjR6NEKxOVg9N2F-
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H157 MmLzSpVv[3Srb36gRZN{[Xl? M2DHWVVuVQ>? NYm0bm81Omh? M{TWTpN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ6NU[yPEc,OjV{OEW2Nlg9N2F-
A549  NGPO[49HfW6ldHnvckBCe3OjeR?= MYW1cW0> MnPNNog> NWG5XG1pe3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK NEDHVI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK4OVYzQCd-MkWyPFU3Ojh:L3G+
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NBL-W-S  MXTBdI9xfG:|aYOgRZN{[Xl? NXvXSlBzOW2P Mn75Oog> MnLubY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgS2FPXC14MR?= MlLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MkOyNlIoRjJ3M{KzNlIzRC:jPh?=
NBL-W-S  M{HYO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfkNY1O M4jX[FZp NI[ySoVmdmijbnPld{BISU6WLU[xJJRwgGmlaYT5 NGLlVXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOyN|IzOid-MkWzNlMzOjJ:L3G+
A549  NGnWfmhCeG:ydH;zbZMhSXO|YYm= MmSxOY1O NXn0bG4xOmh? NUDZXGVwTE2VTx?= NWHUOnFocW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo NV7NUox2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NVY2PjFpPkK1O|E3PTZzPD;hQi=>
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A549  M1TwWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1Ls[FNuVQ>? MoPiNog> M{HQeGROW09? MlTsdoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? M1\EelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{G2OVYyLz5{NUexOlU3OTxxYU6=
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Nara-H MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVu1cW0> NX7rdotVPDiq MkLG[Y5p[W6lZYOgeIVue2m{b3zpcZV{dWWmaXH0[YQhe3WycILld5Nqd25ib3[gUoFz[S2KIHPlcIwheHKxbHnm[ZJifGmxbh?= NEfDeVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwOVA{Oyd-MkG4NFUxOzN:L3G+
HUVECs M2HUe2Z2dmO2aX;uJGF{e2G7 MlW1NVBuVQ>? NUXPZmlqOC53aB?= NIPLPXhl\WO{ZXHz[ZMhfGinIFHHSU1DW0GrbnT1Z4VlKGG3dH;wbIFogSCuZY\l MofLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2Nki1PVIoRjJzNE[4OVkzRC:jPh?=
HepG2 NH;DNHFCeG:ydH;zbZMhSXO|YYm= MWWycW0> MnzPNYg> MU\lcohidmOnczDyZYRq[XSrb36tbY5lfWOnZDDj[YxtKGSnYYTo M4fRTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEWzOlkyLz5{MUS1N|Y6OTxxYU6=
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A673 NX3TSXQxeUiWUzDhd5NigQ>? NEn1WHFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY Mln4dWhVWyCjc4PhfS=> M{DoRpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy| NFjqWXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 NVHJS29PeUiWUzDhd5NigQ>? Mmi0dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= NEXrRlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-37 NI\UbopyUFSVIHHzd4F6 M3TWZpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= MmDaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD NUfjVWlIeUiWUzDhd5NigQ>? MUfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> MlPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH NYracoJFeUiWUzDhd5NigQ>? NIO2TWtyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 M1m2[pFJXFNiYYPzZZk> NXXqR405eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz M1i1W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) NFH5flByUFSVIHHzd4F6 MkXQdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| NWTJOIh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 NIXzN5RyUFSVIHHzd4F6 NITEOpZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz M2fOVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 M2j1cJFJXFNiYYPzZZk> MWrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| Ml;GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh41 NXH6WlNieUiWUzDhd5NigQ>? MUnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? M1fzeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh30 M1eyeZFJXFNiYYPzZZk> NVztVmtZeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoN|Ah[2WubIO= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 MknNdWhVWyCjc4PhfS=> NWfSUnMzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM> NFmyPIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC Mmq3dWhVWyCjc4PhfS=> NGL3SYpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> MojJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 MoDndWhVWyCjc4PhfS=> MofSdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= M3fuWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 NE\scm1yUFSVIHHzd4F6 MnLSdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 MmjzdWhVWyCjc4PhfS=> NX7FT4h{eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoNVgh[2WubIO= NXLnTmdpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Assay
Methods Test Index PMID
Western blot α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 ; caspase-3 / caspase-9 / PARP ; VEGF ; APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2 29296191 25891159 26316755
Immunofluorescence LC3 / Hif-α / COX2 29039446
Growth inhibition assay Cell viability 26934124
In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol (from reference)

Kinase Assay:[4]
  • Protein degradation assay:

    HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.

Cell Research:[2]
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
  • (Only for Reference)
Animal Research:[5]
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 10 mg/mL warmed
(67.04 mM)
Ethanol 4 mg/mL
(26.81 mM)
DMSO 3 mg/mL warmed
(20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage 3 years -20°C powder
Smiles CN1C=NC(=N)C2=C1N=CN2

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

Answer:
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

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