3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULETHJbO22P NUS2eVJHOmh? M3yzZWROW09? NVjLc4NqemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= NXPZSXlkOjV5MU[1OlE>
A549  M{nPd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHqxVpQ{dU1? M{D6UFJp M2HwOGROW09? NWfkPXNWemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= NXzWWZZvOjV5MU[1OlE>
95D NVvle5hZSXCxcITvd4l{KEG|c3H5 MWO1cW0> NFS0ZWYzcA>? M3G2emROW09? NYr6TnFncW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo M3P0NlI2PzF4NU[x
A549  M{X6WGFxd3C2b4Ppd{BCe3OjeR?= MmLhOY1O M2\h[|Jp NVnPSZNvTE2VTx?= MnTubY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq NI\YbVAzPTdzNkW2NS=>
NBL-W-S  MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYSxcW0> NGnEPFI3cA>? NEnP[GRmdmijbnPld{BISU6WLU[xJJRwgGmlaYT5 M1PtPFI2OzJ|MkKy
NBL-W-S  MYPBdI9xfG:|aYOgRZN{[Xl? MXSxcW0> MXy2bC=> NWH2PYxMcW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhT0GQVD22NS=> NUHIXnZ3OjV|MkOyNlI>
A2780cp  MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rJTlFuVQ>? M2H5blFp MWjpcoNz\WG|ZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MmDWNlU{OjJ4OUS=
A549  M1vvO2Z2dmO2aX;uJGF{e2G7 MYC1cW0> MmLvNog> MmDZd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ MYmyOVI5PTZ{OB?=
H157 NXf2ZmJFTnWwY4Tpc44hSXO|YYm= NY[2VmxpPW2P M1e1clJp Mnnmd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ MkjyNlUzQDV4Mki=
MDR MnPpRZBweHSxc3nzJGF{e2G7 MoriNVBuVQ>? M{HFVVZp Mmr6d5Rz\W6pdHjlcpMhfGinIIDve4VzKG:oIHHueIlk[W6lZYKg[JJ2\3N? NYnkSGE4OjVyMUm3NFE>
HT-29  NHzHWoRCeG:ydH;zbZMhSXO|YYm= NIizenMydU1? MVO0PIg> MXfEUXNQ NHH3XHFmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NY\sUWVrOjR6NEKxOVg>
Microglia  M4HCVmFxd3C2b4Ppd{BCe3OjeR?= M321UVVuVQ>? NHfEPGczPGh? Mn\Y[IVkemWjc3XzJIh6eG:6aXGtbY5lfWOnZDDj[YxtKGSnYYTo MU[yOFgyQDZyMR?=
A2780cp MkH6RZBweHSxc3nzJGF{e2G7 NFnhU2MzNjWvTR?= NYrDRo1ZOWh? NXLafnJK\GSKMl:= MWPlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo NXOyenZ6OjR6MUe5OFY>
HepG2 NGnESWZHfW6ldHnvckBCe3OjeR?= M3fjXFVuVQ>? NYC3UIhGPGh? MWPpcoNz\WG|ZYOgTGwhemWuZXHz[S=> MUmyOFcyOzV6Nx?=
A549 MnHiRZBweHSxc3nzJGF{e2G7 M2ntd|VuVQ>? NUfFSllXPDiq MonS[IVkemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGxiZHXheIgh[W6mIHX4dJJme3Orb36gcIV3\Wy|IH;mJINie3Cjc3WtN{whSmWlbHnuMVEh[W6mIFzDN{1KUQ>? NHTk[FYzPDdyNkOwNy=>
U2OS M2n1fmFxd3C2b4Ppd{BCe3OjeR?= M1\rSlExdU1? MknzNlRp M2LvV4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? NFvzTlczPDZ|OUCxNy=>
Saos-2 Mn\iRZBweHSxc3nzJGF{e2G7 NE[wb4syOG2P NV[5WGN2OjSq MljNbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJJRp\SCXMl;TJINmdGy|IHnu[JVk\WRiYomgSI95 NEjhWXMzPDZ|OUCxNy=>
A549 NXi5U|hLSXCxcITvd4l{KEG|c3H5 M1PaNlExdU1? NWfXT4FMPDiq NIjWc4ti[2OnbHXyZZRmeyC2aHWgdoVlfWO2aX;uJI9nKGOnbHygeoli[mmuaYT5JIlv\HWlZXSgZpkhWFS[ NWP6WJB7OjR4Mk[3NlI>
HCT116  NUjTe|BnSXCxcITvd4l{KEG|c3H5 NXP6WllEPW2P MVeyOIg> MYTlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? MWiyOFYzPjV{Mh?=
HGC-27 NXjoeIg3TnWwY4Tpc44hSXO|YYm= NX33c4ZqOTCvTR?= NEPXWoIycA>? NIL6SJVqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkhdG:|czDifUBTSURyMEGgc5IhVUtvMkKwOi=> NWjNNnZlOjR2MU[zOFk>
U2OS  NI\yZZJHfW6ldHnvckBCe3OjeR?= M4jtWFAvPS9zbV2= Mk\pOFhp NVrwVFV7cW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? Ml7DNlQ{PTh|NEK=
MG-63 MVTGeY5kfGmxbjDBd5NigQ>? M3WyZlAvPS9zbV2= MkjvOFhp M1TMcYlv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NUHsT244OjR|NUizOFI>
MG-63 M36zfGFxd3C2b4Ppd{BCe3OjeR?= NV2yPXROOC53L{HtUS=> NV;WfGQzOzKq MVrlcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| M4fRTFI1OzV6M{Sy
MG-63 Ml[3RZBweHSxc3nzJGF{e2G7 MoXKNVBuVQ>? M4m0dlEzcA>? NUG1TVl1\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? NX\KOmNKOjR|NUizNFE>
NTUB1 MnrCRZBweHSxc3nzJGF{e2G7 MlTvOY1O MkPENU42cA>? MUPwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> MXiyOFM1QTF5Nh?=
T24 M4fTbGFxd3C2b4Ppd{BCe3OjeR?= NVTwd4VJPW2P NH:3Z2oyNjWq MUPwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> MoniNlQ{PDlzN{[=
SGC-7901  MnfSRZBweHSxc3nzJGF{e2G7 MUmycW0> MVmxbC=> NHLDdVZqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= NIPre4czPDN{MUO0NC=>
SMMC-7721 M3zpVmFxd3C2b4Ppd{BCe3OjeR?= MVqycW0> NFnYb5cycA>? NFvkZ25qdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= NFfGNWMzPDN{MUO0NC=>
ECSCs  MV3BdI9xfG:|aYOgRZN{[Xl? MWSxNI1O MnXzOIg> MXnk[YNz\WG|ZYOgdoFx[W27Y3nuMZRz\WG2ZXSgZZBweHSxc3nz NUexZnprOjR|MUmxNFk>
MCF-7  M4r0SWZ2dmO2aX;uJGF{e2G7 NYrBVGI{OTCvTR?= NYrIbHRYOjSq NYKwSY1lcW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JINp\W2xdHjldoFxgSCmcoXndy=> NFXkZo0zPDNzNUW3PC=>
UOK257 M3n0SGFxd3C2b4Ppd{BCe3OjeR?= Mn[0OY1O M3HwTVNp NX25dmJ4\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= M{KyV|I1OzB3NkC0
ACHN-5968 NGe4WFRCeG:ydH;zbZMhSXO|YYm= M{jvRlVuVQ>? M4HFTVNp NE\wOYpmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NV\OUFFHOjR|MEW2NFQ>
Huh7 NXXxeZZKSXCxcITvd4l{KEG|c3H5 MWiycW0> MXqxNog> MWLEUXNQ MkLHbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= NF\OXWMzPDJ7N{OwNC=>
Hep3B MX3BdI9xfG:|aYOgRZN{[Xl? Mle2No1O NEfyS3EyOmh? NVPhSo5zTE2VTx?= MVPpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= NVzvcGx3OjR{OUezNFA>
RKO NGDmWXNHfW6ldHnvckBCe3OjeR?= NE[3SFQzdU1? NWDRbmZROWh? NIf2W2xFVVOR Mofh[Y5p[W6lZYOgZ4VtdCCmZXH0bEBjgSCpZXzkZY5idXmlaX6= MYCyOFI6OTd5Nx?=
HepG2 E47 M2PtXWZ2dmO2aX;uJGF{e2G7 MmHxNk42dU1? M3Xsb|Q5cA>? MmCxbY5kemWjc3XzJJRp\SC2b4jpZ4l1gSCxZjDBRUwhSlORLDDhcoQhS0OuNB?= NE\uVVAzPDJ5M{ezPC=>
LoVo  NGC4dXZCeG:ydH;zbZMhSXO|YYm= NEjpZWU2dU1? NYPCZ4FoPDiq MmXl[Y5p[W6lZYOgSGNCNWmwZIXj[YQh[XCxcITvd4l{Ng>? MoXENlQzODF6MUK=
WiDr NWTablM{TnWwY4Tpc44hSXO|YYm= NXWzeWEzOTCvTR?= MUWxbC=> M3zQUYlvcGmkaYTzJHBESkxvaX7keYNm\CCOQ{OgTWkh\XiycnXzd4lwdg>? M1;5S|I1OTlyNEi5
H1299  NHXE[oFHfW6ldHnvckBCe3OjeR?= MVuxNI1O NIHZbIk1cA>? MoXlbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NF:2VlMzPDF5M{KwPC=>
H460 NEPKUnpHfW6ldHnvckBCe3OjeR?= MoDkNVBuVQ>? MljlOIg> M2rUbolv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NHW1WIYzPDF5M{KwPC=>
A549 MmTVSpVv[3Srb36gRZN{[Xl? NWLsVpF2OTCvTR?= NGDLUoQ1cA>? NUDPWVBRcW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliaYLyZYRq[XSrb36= MXeyOFE1Ojd|NR?=
Hep3B MULBdI9xfG:|aYOgRZN{[Xl? Mo\EOY1O M1vU[|I1cA>? Mn;iZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz MkPjNlQxPjZ4OUO=
SMMC-7721 NYm5XWdbSXCxcITvd4l{KEG|c3H5 NYnjcG5{PW2P MWKyOIg> MVvheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= NGLIN4MzPDB4Nk[5Ny=>
HO8910 NW\rXplVSXCxcITvd4l{KEG|c3H5 M1LSU|ExdU1? MVKxNog> NUmyVI5L\W6qYX7j[ZMhSjF7LXnu[JVk\WRiYYDvdJRwe2l? MX6yN|k5OzZzMB?=
MCF7 NHzkS4lHfW6ldHnvckBCe3OjeR?= MlrUOY1O NW\Jd|ZwOjSq Mm\xbY5kemWjc3XzJGN2VyCrbnT1Z4VlKGOnbHyg[IVifGh? MY[yN|k3OjZ{OR?=
HONE-1  NW\hSVl4TnWwY4Tpc44hSXO|YYm= MVK1cW0> NWnsWIFZOWh? NWDveIU6emWycnXzd4V{KD[{LX3l[IlifGWmIGLPV{Bxem:mdXP0bY9v M2nROlI{QDl{M{W4
HeLa MkfrSpVv[3Srb36gRZN{[Xl? NULte|hOOTCvTR?= M1fodlJp NXPnfFd5e3WycILld5NmeyCOQ{OgTWkh\XiycnXzd4l{d25? M{LxdVI{QDZ2N{O4
HepG2 NFm4PXNHfW6ldHnvckBCe3OjeR?= M4C3fVExdU1? MYmyOIg> NWLIe|hxcW6qaXLpeJMhe2mWSVfBVk0h[W6mIFjCV3MucW6mdXPl[EBifXSxcHjh[5k> Mo\2NlM5OTdyNEC=
SH-SY5Y M{\tSmZ2dmO2aX;uJGF{e2G7 M4T2V|FuVQ>? NUXjeZNmOjSq NUTlRoV7cW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JHRQS1B? MkToNlM4PDNzNEi=
SH-SY5Y NEjUcFNCeG:ydH;zbZMhSXO|YYm= NXnEO5hbPW2P M3\YSlFp NV:xR4E5[WKxbHnzbIV{KGOnbHHzeJJwdCCwZYXyc5Bzd3SnY4TpeoUh\W[oZXP0 NY\6U2puOjN4MUmzPVU>
PC12  MVTGeY5kfGmxbjDBd5NigQ>? M{\TS|ExdU1? NYPDVZBMOjSq NU\FbIVSf2G2ZYK= NV;kVJRTyqCrbnjpZol1eyClaIntc5RzgXC|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6= MlPzNlM3ODN7N{m=
OV2008 NHjiWFFCeG:ydH;zbZMhSXO|YYm= M{\tWVVuVQ>? NUXvNI57OjSq MVLjc453\XK2czDGWHk4OjBid3n0bEBETESSIHnueI8h[W5iYXTkbZRqfmViZX\m[YN1KHSxd3Hy[JMhc2mubHnu[{BwfmG{aXHuJINidmOncjDj[Yxtew>? M1\BbVI{PTl{Mkix
A2780 MoHFRZBweHSxc3nzJGF{e2G7 NYnJRWpSPW2P MlvkNlRp MlfGZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? NUnRT5BPOjN3OUKyPFE>
Saos-2  NYK4ZnZjSXCxcITvd4l{KEG|c3H5 M2nFS|FuVQ>? NXW4RpA2QT[q MXXpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KFCFWB?= MmjrNlM2PjNzN{G=
1321N1 MoWwR5l1d3SxeHnjbZR6KEG|c3H5 MoXaOY1O M2\3SFI1cA>? NHH2boxxem:2ZXP0d{Bk\WyuIHHnZYlve3RiUFPOMYlv\HWlZXSgeI95cWOrdIm= M3LiPVI{PTJ3Mk[1
SH-SY5Y MYPDfZRwfG:6aXPpeJkhSXO|YYm= MYe1cW0> M4i2eVI1cA>? NYmyXGF1cW6lcnXhd4V{KFCFTjD0c5hq[2m2eR?= M{TC[|I{PTJ3Mk[1
HT-29  NU[0ZnBFTnWwY4Tpc44hSXO|YYm= MYexcW0> MofVOFgwQT[q MVLpcohq[mm2czDBUXBMKGmwZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSq MXOyN|UxQDJ5Mh?=
OR6 Mk\iSpVv[3Srb36gRZN{[Xl? NV7x[2lOOTCvTR?= M1O3[FczcA>? NVi5SZNIe3WycILld5NmeyCKQ2[gdoVxdGmlYYTpc44h[W6mIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= MUWyN|M6PTh5NR?=
Hela  MmDnSpVv[3Srb36gRZN{[Xl? MUe1cW0> M1njW|I1cA>? NX\LZ|BFcW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? MkXGNlM{QTV4N{m=
MCF-7  NEXhOopHfW6ldHnvckBCe3OjeR?= NIjJSXU2dU1? MVKyOIg> MoHkbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> NF2wNVIzOzN7NU[3PS=>
HUVECs Mnv5SpVv[3Srb36gRZN{[Xl? MXyzcW0> MYWyOIg> M{fRPIJtd2OtczD0bIUheHKxdHXjeIl3\SCnZn\lZ5Qhd2ZicnXzeoVz[XS{b3ygZpkhcW6qaXLpeIlv\yCjdYTvdIhi\3l? NIP3cZAzOzN3OEmyPC=>
T24 NH6z[JZHfW6ldHnvckBCe3OjeR?= MWixNI1O MWmxbC=> MmO5doVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= MlHuNlM{PTRyOEC=
U251MG  M3vyOmZ2dmO2aX;uJGF{e2G7 NITOdVc{dU1? NE[yZZQycA>? MWDzeZBxemW|c3XzJGxEOy2LSTDwdo91\WmwIHX4dJJme3Orb36= MmHRNlM{Ozh4MUi=
GTL-16  M4rrNWFxd3C2b4Ppd{BCe3OjeR?= MWS1cW0> NV;4NlAxOjSq NFXEeYZz\WS3Y3XzJINmdGxidnnhZoltcXS7IHHzJINwdXCjcnXkJJRwKGOnbHzzJJRz\WG2ZXSge4l1cCCPRWSgbY5pcWKrdH;ydy=> NHKw[VQzOzNzM{S5NC=>
T-47D NF\uWGNHfW6ldHnvckBCe3OjeR?= NUnEV3FNOTCvTR?= M{CyTVJp MWjpcohq[mm2czDheZRweGijZ4mgdJJw[2W|czDhcoQhcW6lcnXhd4V{KHKjcHHtfYNqdiCrbnT1Z4VlKGGyb4D0c5Nqew>? MnW1NlM{ODByMk[=
PaCa44 MUDBdI9xfG:|aYOgRZN{[Xl? MoDKNk42dU1? MnHNNYg> MULy[YR2[2W|wrDn[Y5qeGmwLX3l[IlifGWmIHHwc5B1d3Orcx?= M4P0clI{OTJ2MUGy
MDA-MB231 NFX1ZnFHfW6ldHnvckBCe3OjeR?= M1n0W|VuVQ>? MYKxbC=> MnHubY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> NGnMe|kzOzB6OEi1NC=>
HeLa NILGZWVCeG:ydH;zbZMhSXO|YYm= NHrEeVQyOG2P MY[ybC=> MVzk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSvZX70JJdqfGhiUFXJ NFLXfFEzOzByMEGzOS=>
SK-HEP-1 M1X5NmFxd3C2b4Ppd{BCe3OjeR?= M3PyXlExdU1? M37yOFFp MV3wdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz M2TX[lIzQDV6NkS5
HepG2 MYfBdI9xfG:|aYOgRZN{[Xl? M4fSUlNuVQ>? NGG0fHI2cA>? NH;MdHpz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgVWR{ MoG4NlI5OzZ3OUW=
MCF-7  M33VUWZ2dmO2aX;uJGF{e2G7 NH:0NIQzdU1? NV\uUXFUOjSq NYXaPIF3cW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF5CpC=> NVT4UXhWOjR7N{C2O|Y>
MDA-MB-231 NWrpb29iTnWwY4Tpc44hSXO|YYm= MkLQNo1O M2TKNVI1cA>? M{W4VolvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= NULpW2NCOjR7N{C2O|Y>
MCF-7  M2XJdWZ2dmO2aX;uJGF{e2G7 NXXPcnR[Om2P NFi5[po1QGh? MWfwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> NXX6NYkyOjR7N{C2O|Y>
MDA-MB-231 Mmq1SpVv[3Srb36gRZN{[Xl? MnLwNo1O MkLROFhp MVXwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> MUKyOFk4ODZ5Nh?=
PANC-1  NIfFe4RCeG:ydH;zbZMhSXO|YYm= M3XuNFFuVQ>? M2LsVlQ5cA>? MofRSG1UVw>? Mnfq[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> M{nwV|I1QDR{MUW4
MDA-MB 231 M3LOTWFxd3C2b4Ppd{BCe3OjeR?= MVG1cW0> NX31dnNsOC53aB?= M4iwe41w\HWuYYTld{BVd2OxbXnuxs7DqGmwZIXj[YQh[XCxcITvd4l{ MV:yOFg{ODd6MR?=
Microglia  NEfaS5FCeG:ydH;zbZMhSXO|YYm= MmTYOY1O NIq3OIEzPGh? NHTCZYZl\WO{ZXHz[ZMhcHmyb4jpZU1qdmS3Y3XkJINmdGxiZHXheIg> Ml7oNlQ5OTh4MEG=
A2780cp NEXMeWVCeG:ydH;zbZMhSXO|YYm= NUH0[IRFOi53bV2= M1LhW|Fp NFvVR2pl\Eh{Tx?= M3fhU4VvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MnjWNlQ5OTd7NE[=
HepG2 Mmn6SpVv[3Srb36gRZN{[Xl? M1LJdFVuVQ>? MWG0bC=> MVfpcoNz\WG|ZYOgZ4VtdHWuYYKgcIV3\Wy|IH;mJGhNyqB? MV2yOFcyOzV6Nx?=
U2OS M{\BVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYWxNI1O Mk\0NlRp NVnWTHZlcW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJIlv\HWlZXSgZpkhTG:6 NI\GPHozPDZ|OUCxNy=>
HCT116 MW\BdI9xfG:|aYOgRZN{[Xl? MWm1cW0> NHLKNVEzPGh? NHvYfI9FVVOR Mo[w[Y5p[W6lZYOgZZBq\2WwaX6tbY5lfWOnZDDj[YxtKGSnYYTo NIrDTZUzPDZ{NkWyNi=>
MCF-7  MkjoRZBweHSxc3nzJGF{e2G7 MXKwMlFuVQ>? MYC2bC=> NYnWSY1p\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> M3frSlIzPzVzOUi5
PC-3  NX7ZeG1wSXCxcITvd4l{KEG|c3H5 NXzMO5FMOm2P M3q4N|Jp M{jqOolv[3KnYYPld{BQWklvaX7keYNm\CClZXzsJIRm[XSq NYT4SIgyOjJ5NEW1PFA>
U251  NHGwN|BCeG:ydH;zbZMhSXO|YYm= NV[4e3oyPW2P MXiyOIg> MoPnbY5kemWjc3XzJHMyNWmwZIXj[YQh[2WubDDk[YF1cA>? NUPSNYtlOjJ3N{m3PFg>
HeLa  NGnrS4pCeG:ydH;zbZMhSXO|YYm= NEHnNWc2dU1? MVKyOIg> NWLzU|ZbcW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp M4rTZVIzPTR3MUK4
A549 M2CybGZ2dmO2aX;uJGF{e2G7 MWWwMlFuVQ>? NILlU|EzPGh? M13a[5N2eHC{ZYPz[ZMhW1VzMUK3OE1qdmS3Y3XkJINmdGxiZHXheIg> NGTsfYUzOjR4Nkm2NC=>
pDCs NF3CcZRHfW6ldHnvckBCe3OjeR?= NXW4bZhIOTCvTR?= M4SyfVAvPWh? MoO2xsBz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gTWZPNc7zIHL5JJN{Wk6DNEC= M1fvWFIzOzl4NUm5
BGC-823 NETwPY1HfW6ldHnvckBCe3OjeR?= MYO1cW0> NEPSWFQzcA>? MljBbY5pcWKrdIOgeIhmKHKjdHWgc4Yh[XW2b4DoZYdq[yClZXzsdy=> MXyyNlMzOjF3Mh?=
U937 NEnlc49HfW6ldHnvckBCe3OjeR?= NVz5XWRUOm2P M{XieFEzcA>? MVHk[YNz\WG|ZYOgeIhmKGG3dH;wbIFogSC{YYTpc:Kh NGTVNHkzOjF3NUG1NC=>
Marc-145 M2rEbGZ2dmO2aX;uJGF{e2G7 M1zT[lVuVQ>? NETyNIEyOi9{ND:zOog> MY\y[YR2[2W|IITo[UBRWlKVVjD0bZRmenNiYX7kJJRp\SCycn;0[YlvKGW6cILld5Nqd25? M3;yUFIzOTF7OUCw
HBx  NV64TWpvSXCxcITvd4l{KEG|c3H5 M4Pw[FExdU1? NYnUPVI1PDiq MkS0SG1UVw>? M{TnNIlv[3KnYYPld{Bk\WyuIHTlZZRp NF\SWlAzOjB{MEC3PC=>
MCF-7 NYLGZXVJTnWwY4Tpc44hSXO|YYm= MmTQNVBuVQ>? NX;0UHZ[PDiq M1r2doJtd2OtczDheZRweGijZ4mgbY5lfWOnZDDifUBjd3K2ZYrvcYlj NFviTlAzOTl|MUmzOy=>
RMPI8226  NXT5bmh6TnWwY4Tpc44hSXO|YYm= M2PUeVVuVQ>? MX[xbC=> NVjQell3e3WycILld5NmeyC2aHWgcIV3\Wxib3[gZZV1d3CqYXf5JJVv\GW{IH71eJJq\W62IHTldIxmfGmxbh?= MUCyNVkyPTZ{MB?=
PC12/TetOn NHywcodHfW6ldHnvckBCe3OjeR?= M1PocFAvOS9zbV2= NF7PTHEyQGh? M4rhOIxm[WS|IITvJO6yNXO7bjjXWEkh[WOldX31cIF1cW:wLDD0c5hq[2m2eTygZY5lKG:uaXfvcYVzKG[xcn3heIlwdsLi M3TrcFIyQTB4NkW5
HeLa  NV3sTlQzS3m2b4TvfIlkcXS7IFHzd4F6 M{POdlJuVQ>? M4rD[FI1cA>? NYDOXGNJcW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv MoK0NlE5PzV|OEW=
Jurkat NFTxS29HfW6ldHnvckBCe3OjeR?= NEPaRmUyOG2P NWjuWIM3OWh? M3zDc4Rm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= MnjhNlE5PjRyM{e=
K562 MULGeY5kfGmxbjDBd5NigQ>? NHL2VG0yOG2P M1zzOFFp NYiwTmJp\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEyFMz3JTUBidmRidHjlJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> MUSyNVg3PDB|Nx?=
Nara-H MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DsUVVuVQ>? M33CdFQ5cA>? MkH6[Y5p[W6lZYOgeIVue2m{b3zpcZV{dWWmaXH0[YQhe3WycILld5Nqd25ib3[gUoFz[S2KIHPlcIwheHKxbHnm[ZJifGmxbh?= Mmr4NlE5ODVyM{O=
HUVECs Mn3HSpVv[3Srb36gRZN{[Xl? M3Tx[|ExdU1? MXuwMlVp MVjk[YNz\WG|ZYOgeIhmKEGJRT3CV2FqdmS3Y3XkJIF2fG:yaHHnfUBt\X[n MX:yNVQ3QDV7Mh?=
HepG2 MmLiRZBweHSxc3nzJGF{e2G7 MWiycW0> MnLMNYg> NELoNXBmdmijbnPld{Bz[WSrYYTpc44ucW6mdXPl[EBk\WyuIHTlZZRp NEjyS4czOTR3M{[5NS=>

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID