Necrostatin-1

For research use only. Not for use in humans.

Catalog No.S8037

37 publications

Necrostatin-1 Chemical Structure

Molecular Weight(MW): 259.33

Necrostatin-1 is a specific RIP1 (RIPK1) inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells.

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Selleck's Necrostatin-1 has been cited by 37 publications

Purity & Quality Control

Choose Selective TNF-alpha Inhibitors

Biological Activity

Description Necrostatin-1 is a specific RIP1 (RIPK1) inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells.
Features A powerful tool for characterizing the role of necroptosis with characterized primary target.
Targets
RIP1 [1]
(293T cells)
490 nM(EC50)
In vitro

Necrostatin-1 (1-100 μM) inhibits the autophosphorylation of overexpressed and endogenous RIP1.It is found RIP1 is the primary cellular target responsible for the antinecroptosis activity of Necrostatin-1. [1] Necrostatin-1 efficiently suppresses necroptotic cell death triggered by an array of stimuli in a variety of cell types. Necrostatin-1, previously identified as small-molecule inhibitor of necroptosis, inhibits RIP kinase-induced necroptosis and inhibits TNF-α-induced necroptosis in jurkat cells with EC50 of 490 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 M{fycmNmdGxiVnnhZoltcXS7IFHzd4F6 MkXqOVDDqM7:TR?= MoXRNlTDqGh? Ml32bY5pcWKrdIOgUW1UNWmwZIXj[YQh[2WubDDk[YF1cA>? MoTRNlY1PzJ5MkO=
MEFs  MmnKR4VtdCCYaXHibYxqfHliQYPzZZk> NGHmNWsyOOLCid88US=> NIfFSoQ1QCCq M{H0RWROW09? NF\NXo5qdmirYnn0d{B7XkGGLYDyc41wfGWmIHTlZZRpKG:oIFPOU3Q{NWSncHzleIVlKE2HRoO= M3TFTFI3PDN5N{i5
BMDM  MoX1SpVv[3Srb36gRZN{[Xl? M4DOVFExyqEQvF2= MX:zNOKhdWmw NVLU[JhyeHKxdHXjeJMh[2WubIOg[pJwdSCWQVvJMYlv\HWlZXSgUGRJKHKnbHXhd4U> NHf2OHczPjN6MU[wNS=>
NCI-H28 MUfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M33lZlExyqEQvF2= MWWyOOKhcA>? NG\sS2hxemW4ZX70d{BFSVCHLXnu[JVk\WRicnXkeYN1cW:wIH;mJG5EUS2KMkigZ4VtdCC4aXHibYxqfHoEoB?= M{DDU|I3ODB2MUO4
MiaPaCa-2 MnnNSpVv[3Srb36gRZN{[Xl? NIrOXJkzOCEQvF2= NXnKXnJKOjRiaB?= Mn\P[IVkemWjc3XzJJRp\SCnYYLsfUBv\WO{b4TpZ{Bk\Wyucx?= MkCyNlYxODB4MEe=
BxPC-3 NWT2Rm5OTnWwY4Tpc44hSXO|YYm= M33XNlIxKM7:TR?= M2rYe|I1KGh? M3jKWYRm[3KnYYPld{B1cGViZXHycJkhdmWlcn;0bYMh[2WubIO= MnXJNlYxODB4MEe=
SGC-7901 NYG2b2hXS2WubDDWbYFjcWyrdImgRZN{[Xl? NIKwZ28{OMLizszN MWmxxsBp MUXzeZBxemW|c3XzJI95[WyrcHzheIlvNW2nZHnheIVlKGOnbHyg[IVifGh? MlX0NlU4PjdyN{[=
KMS-12-PE  Mo\OR4VtdCCYaXHibYxqfHliQYPzZZk> M{T0blYxKM7:TR?= MlTjOUBp Ml;XbY5pcWKrdIOgV2hMNWmwZIXj[YQh[2WubDDk[YF1cA>? MX[yOVU{ODB7OB?=
L929-A  Mk\NR4VtdCCYaXHibYxqfHliQYPzZZk> Mn;MOVDDqM7:TdMg NEWwU2YzPCCq MVPicI9kc3NiVF7G{tEucW6mdXPl[EBk\WyuIHTlZZRp MY[yOVM6QDV2MB?=
L929-N  MmLPR4VtdCCYaXHibYxqfHliQYPzZZk> NV\udoNmPTEEoN88UeKh Mo\lNlQhcA>? M{fvNoJtd2OtczDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGh? NH\DN2wzPTN7OEW0NC=>
L929-A  M{flRmZ2dmO2aX;uJGF{e2G7 NHu4[os2OMLizszNxsA> NV;H[YRVOTJiaB?= M3\JXoJtd2OtczD0bIUh[2ynYY\h[4Uhd2ZiQ3HzdIF{\S1|IHHu[EBRSVKS NUXnTYp7OjV|OUi1OFA>
L929-N  Ml7kSpVv[3Srb36gRZN{[Xl? NXntWVNbPTEEoN88UeKh MnXRNVIhcA>? NX:zVoFo[myxY3vzJJRp\SClbHXheoFo\SCxZjDDZZNx[XOnLUOgZY5lKFCDUmC= MkjRNlU{QTh3NEC=
L929 MV\GeY5kfGmxbjDBd5NigQ>? NF\xXog2OMLizszNxsA> MYKxNkBp M3fXeolvcGmkaYTzJHRPTs7zLXnu[JVk\WRiQnnkJINt\WG4YXfl M1PaZ|I2Ozl6NUSw
L929-A NYKxdlhrTnWwY4Tpc44hSXO|YYm= NXnOTG02PTEEoN88UeKh MUWxNkBp NEe1dIRqdmirYnn0d{B1cGViVF7G{tEucW6mdXPl[EBtd3O|IH;mJI1qfG:laH;u[JJq[WxibXXtZpJidmVicHXycYVi[mmuaYT5 NFq3coQzPTN7OEW0NC=>
L929 NXXuUZJHS2WubDDWbYFjcWyrdImgRZN{[Xl? M{XTN|MxyqEQvF2= NV\CR3hnOcLiaB?= MnPrbY5pcWKrdIOgWG5HNc7zLXnu[JVk\WRibH;zd{Bw\iClZXzsJJZq[WKrbHn0fS=> Mli1NlUxQTV5NEK=
L929 M2Due2NmdGxiVnnhZoltcXS7IFHzd4F6 NYW4R3hiOzEEoN88US=> M{LlPVHDqGh? Mnj6bY5pcWKrdIOgWG5HNc7zLXnu[JVk\WRiY3zlZZZi\2Vib3[gWI9xdyCL NWrtcGpwOjVyOUW3OFI>
Huh7 MWDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHWwcpM2OCEEtV2= NIflVVEzPC92ODDo M3XOTmROW09? MYPwdoV3\W62czDj[YxtKGSnYYToJI9nKHKDZFjDWkBkdy2rbn\lZ5Rm\CCKdXi3JINmdGy| MWqyOFk4OzJ2MB?=
OHC Ml\1SpVv[3Srb36gRZN{[Xl? M4jWWlMxOOLCid88US=> MkP2SG1UVw>? NHPIc4VqdmO{ZXHz[ZMhdm:rc3WtbY5lfWOnZDDjc45l\W6|ZXSgcpVkdGWr NH:1[ZMzPDh5NEezOC=>
OHC MorsSpVv[3Srb36gRZN{[Xl? Mnq1N|Ax6oDLzszN NGjzcZRFVVOR NF63bFhl\WO{ZXHz[ZMhdm:rc3WtbY5lfWOnZDDze49tdGWwIH71Z4xmccLi MVKyOFg4PDd|NB?=
OHC MVXGeY5kfGmxbjDBd5NigQ>? MkLoN|Ax6oDLzszN MXfEUXNQ NVnQW3EzemW|dXz0d{BqdiCjIILl[JVkfGmxbjDv[kBvd2m|ZT3pcoR2[2WmIGLJVFEh[W6mIGLJVFMhcW2vdX7v[ox2d3Knc3PlcoNm MViyOFg4PDd|NB?=
OHC MofmSpVv[3Srb36gRZN{[Xl? NWfxWpZNOzBy4pEJ{txO NETvRZRFVVOR MlHW[IlucW6rc3jld{Bvd2m|ZT3pcoR2[2WmIFHNVGsh[WO2aY\heIlwdg>? NXnzPW1TOjR6N{S3N|Q>
OHC NW\DVFBPTnWwY4Tpc44hSXO|YYm= MWCzNFDjiIoQvF2= MXLEUXNQ NXLnboNocW6lcnXhd4V{KHSqZTDueY1j\XJib3[gZZBweHSxdHnjJG9JS3Nid3n0bI92fCCjbITldolv\yC2aHWgcIV3\Wy|IH;mJGNEQCCjZoTldkBvd2m|ZTDlfJBwe3W{ZR?= MmnENlQ5PzR5M{S=
SK-Hep1 MlPXSpVv[3Srb36gRZN{[Xl? Mn3HOlDjiIoQvF5CpC=> M2nwN|E56oDLaB?= NXj5OW9Q[myxY3vzxsDPui2uYYDhZ4hwdmVvaX7keYNm\CCvb4LwbI9td2erY3HsJINp[W6pZTygZ4VtdCCmZXH0bEBidmRiUFmgeZB1[Wun MYSyOFg{OjZyMh?=
SK-Hep1 NGThR4tHfW6ldHnvckBCe3OjeR?= NVPITGZFPjEkgJpOwG3DqA>? M2\CblE56oDLaB?= MlvZbY5pcWKrdIOg{tIuVGGyYXPoc45mNWmwZIXj[YQhdGWja3Hn[UBw\iCKTVfCMVHDqA>? M{Pi[lI1QDN{NkCy
SK-Hep1 NEPlXolHfW6ldHnvckBCe3OjeR?= NVmzTGVmPjEkgJpOwG3DqA>? MXuxPQKBkWh? MYPicI9kc3QEoN8yMYxieGGlaH;u[U1u\WSrYYTl[EBRSVJiYXPjeY12dGG2aX;uJIFv\CCDSV[geJJidnOub3PheIlwdiC2bzD0bIUh[3m2b4PvcOKh M33EclI1QDN{NkCy
L-540  NIr6V41HfW6ldHnvckBCe3OjeR?= M4PD[FYx6oDLzszt M4HmelHjiImq NH;5RWZxemW4ZX70d{B1cGViZ3Xu[ZJifGmxbjDv[kBTV1N? M{\aNlI1PTZzNUG5
L-540  NEfRVndHfW6ldHnvckBCe3OjeR?= NEmzeY43OOLCid88cS=> M2rldlHjiImq M4m5Z5Bz\X[nboTzJJRp\SCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJIRmeG:uYYLpfoF1cW:w M4DjZ|I1PTZzNUG5
L-540  NEDKeWhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MX62NQKBkc7:bR?= NYrBNGVFOeLCiXi= NUTjPW45emWmdXPld{B1cGViR3n2bY5we3SjdD;Tc5Ji\mWwaXKtbY5lfWOnZDDj[YxtKGSnYYTo NYf1cYVGOjR3NkG1NVk>
AGS M1HCWWNmdGxiVnnhZoltcXS7IFHzd4F6 MYm2NQKBkc7:bR?= MWWx5qCKcA>? NEWy[ZJxemW4ZX70d{B{cGmtb37pck1qdmS3Y3XkJINmdGxiZHXheIg> M{fHfVI1PDZ|MUm5
NRK-52E  M4nWNmNmdGxiVnnhZoltcXS7IFHzd4F6 NGL4R4MzOCEQvF2= NWS3Rmw6OjRiaB?= NUXJUVV1eHKxdHXjeJMh[2WubIOg[pJwdSClZXzsJIRm[XSqIHPheZNm\CCkeTDpd4Np\W2rYTDpcop2enl? MV:yOFM2OTh2NR?=
NRK-52E  Mk\zR4VtdCCYaXHibYxqfHliQYPzZZk> MmXYNlAh|ryP NXfXdopsOjRiaB?= NEPlN3ZqdmO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiVF7GMe6yKFO2aX31cIF1cW:wIHHu[EBCXFBiRHXwcIV1cW:w MWOyOFM2OTh2NR?=
NRK-52E  M3HSfmNmdGxiVnnhZoltcXS7IFHzd4F6 M1PlXVIxKM7:TR?= NIHsd|AzPCCq M33tXolvcGmkaYTzJIlv[3KnYYPl[EBFenBzIIDyc5RmcW5iZYjwdoV{e2mxbjDh[pRmeiCWTl[t{tEhW3SrbYXsZZRqd25iYX7kJGFVWCCGZYDs[ZRqd25? NHWw[JozPDN3MUi0OS=>
L929 NXfiT3pYTnWwY4Tpc44hSXO|YYm= MY[yM|XDqM7:Zz;tcOKh MYWyOOKhcA>? Ml\ZdoV3\XK|ZXSgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IGTOSu6yKGGub37lJIF{KHenbHygZZMhXE6IzsJCpEvDqHqYQVS= MYeyN|k1OTd4OR?=
L929 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLmZnozNzYEoN88[{9udMLi MYOyOOKhcA>? NXfyb5NPemW4ZYLz[ZMhfGinIHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W6mIHPlcIwh\GWjdHigbY5lfWOnZDDifUBVVkcQsTDhcI9v\SCjczD3[YxtKGG|IGTOSu6yyqBtwrD6WmFF M3TEOVI{QTRzN{[5
L929 NGDoRm5HfW6ldHnvckBCe3OjeR?= NHnEdZozyqEQvHevcYzDqA>? NVm3UYduOjUEoHi= M17RV5Bzd22xdIOgZ4F{eGG|ZT22JEhxOjBrIHHjeIl3cXS7IHHu[EBxem:lYYPwZZNmNTZiY3zlZZZi\2V? NXXnSnFbOjN7NEG3Olk>
L929 NVS0c4t[TnWwY4Tpc44hSXO|YYm= NH[0Xmc2yqEQvHevcYw> NFjXTWwzPMLiaB?= NE[3PIdjdG:la4OgfnZCTCCrbnT1Z4VlKG6nY4LvdJRwe2m|IHHu[EBifXSxcHjh[5k> NXjSXJBqOjN7NEG3Olk>
C6 NYjSSHJpS2WubDDWbYFjcWyrdImgRZN{[Xl? M{L2OVEhdW2xbD;M MV[zJIg> MWnheJRmdnWjdHXzJHNpcWuxbnnuJIlv\HWlZXSg[4xqd22jIHPlcIwh\GWjdHi= NEf6NpMzOzh2MES0NS=>
U87 M3\DbGNmdGxiVnnhZoltcXS7IFHzd4F6 M1j1WVEhdW2xbD;M NYjv[YxOOyCq NFm1R|RifHSnboXheIV{KFOqaXvvcolvKGmwZIXj[YQh\2yrb33hJINmdGxiZHXheIg> MojwNlM5PDB2NEG=
C6 M2XhVmN6fG:2b4jpZ4l1gSCDc4PhfS=> NW\xUZBOOSCvbX;sM2w> MWSzJIg> MmLGZoxw[2u|IIPobYtwdmmwIHnu[JVk\WRibnXjdo9{cXN? M3zmUVI{QDRyNESx
U87 NH\Hd5JEgXSxdH;4bYNqfHliQYPzZZk> MV6xJI1ud2xxTB?= M{TDSFMhcA>? NYLuUoN2[myxY3vzJJNpcWuxbnnuJIlv\HWlZXSgcoVkem:|aYO= MVeyN|g1ODR2MR?=
C6 MVTGeY5kfGmxbjDBd5NigQ>? MkX2NUBudW:uL1y= NX7KRVJnOS53LUOgbC=> Mmrud5VxeHKnc4Pld{B1cGViZYjwdoV{e2mxbjDv[kBTUVBvMTDjZZV{\WRiYomgd4hqc2:waX6= NXy2bFdqOjN6NEC0OFE>
U87 MojwSpVv[3Srb36gRZN{[Xl? M2\yO|EhdW2xbD;M MkH6NU42NTNiaB?= MWTzeZBxemW|c3XzJJRp\SCneIDy[ZN{cW:wIH;mJHJKWC1zIHPheZNm\CCkeTDzbIlsd26rbh?= NVviPYxDOjN6NEC0OFE>
TE671 Ml7rR4VtdCCYaXHibYxqfHliQYPzZZk> Mk[0OFDjiIoQvHevcYzDqA>? Mn21NlQhcA>? M1j0epJme2O3ZYOgS3gyPS1yN{CtbY5lfWOnZDDsc5N{KG:oIHPlcIwhfmmjYnnsbZR6 M1HT[lI{PzR2Mkm2
RMS13 NYfhUJA1S2WubDDWbYFjcWyrdImgRZN{[Xl? M4L2e|Qx6oDLzsznM41tyqB? NYPCO3JmOjRiaB?= M3HE[5Jme2O3ZYOgS3gyPS1yN{CtbY5lfWOnZDDsc5N{KG:oIHPlcIwhfmmjYnnsbZR6 NHHPVVczOzd2NEK5Oi=>
MEFs M3jhZWN6fG:2b4jpZ4l1gSCDc4PhfS=> MXGyM|YwOjBizszN NGDMc3gyQCCq MXfpcohq[mm2czDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGhiaX6gVoVtSSCNTzDNSWZ{ M3mxd|I{PzJ5NUix
MEFs NXHk[3Y6TnWwY4Tpc44hSXO|YYm= NGrtenYzOMLizszN Mn3LNU8zNzRiaB?= Mnvpd5VxeHKnc4Pld{BVVkcQsT3pcoR2[2WmIGLJVGsyKHCqb4PwbI9zgWyjdHnvci=> NFLCeIIzOzd{N{W4NS=>
ΔN-Karpas 299  NHf2XmlEgXSxdH;4bYNqfHliQYPzZZk> MViyNOKh|ryP NFvHVVUyPiCq MnXDbY5pcWKrdIOgR2Q{OC2rbnT1Z4VlKGOnbHyg[IVifGh? M1ztZ|I{PTR3OUO4
MM.1S  M4PUSmN6fG:2b4jpZ4l1gSCDc4PhfS=> MknPPVAhyrWP NYLiXohQOSCq NXPtdm4z[myxY3vzJGJCYSBzMT23NFgzKGmwZIXj[YQhemGyaXSgZ4VtdCC|d3XscIlv\w>? M1n3NVI{PTJ5MUW0
KMS-12-BM MlK1R5l1d3SxeHnjbZR6KEG|c3H5 MV[5NEDDvU1? NHfMVJIyKGh? NFzaN3hjdG:la4OgRmF[KDFzLUewPFIhcW6mdXPl[EBz[XCrZDDj[YxtKHO5ZXzsbY5o NFL0ZW8zOzV{N{G1OC=>
HT-22  MkL4R4VtdCCYaXHibYxqfHliQYPzZZk> MnTSNVDDqM7:TR?= MXexNuKhcA>? MXnEUXNQ M2HYW5Bzd3SnY4TzJIFo[Wmwc4Sg[4x2fGGvYYTlMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NHvNZpIzOzNyN{e1Ni=>
HT-22  NVX5UGw3TnWwY4Tpc44hSXO|YYm= MYKyOeKh|ryP NW[4RXBOOOLCk{OwxsBucW5? Mnr4SG1UVw>? M3rtZ4lvcGmkaYTzJGVTUyCDY4TpeoF1cW:wIHnu[JVk\WRiYomg[4x2fGGvYYTl MmH5NlM{ODd5NUK=
NIH3T3  M2\iUmZ2dmO2aX;uJGF{e2G7 NX7WXlNQOTBxNUCg{txO Ml24NU8{KGh? NWP1Vm5y[W2nbHnvdoF1\XNiVF7G{tEu\HKrdnXuJINwdXCuZYig[o9zdWG2aX;u M{DpUVI{OjZzNke3
SH-EP NXL3WXJ3SXCxcITvd4l{KEG|c3H5 MVOxNQKBkc7:TdMg M2rFTlcz6oDLaNMg MkHzbY5pcWKrdIOgTWFRKGmwaHnibZRwei1iYX7kJGxmgGG2dX31cYFjNWmwZIXj[YQh[XCxcITvd4l{ M1m3PFIzQDlyM{Ky
HL60 NHTMfmRCeG:ydH;zbZMhSXO|YYm= M3PYWlYxKM7:TR?= M{PxXlEzKGh? NXz3V3p5\W6qYX7j[ZMhe2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZM> NWPQSWJSOjJ6M{e2PFk>
HL60/Adr NVLGXpJlSXCxcITvd4l{KEG|c3H5 Mn;COlAh|ryP MmjkNVIhcA>? MmHQ[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= MVWyNlg{PzZ6OR?=
K562 NGjNSXJCeG:ydH;zbZMhSXO|YYm= NIXtZ4Q3OCEQvF2= NF3SSJEyOiCq MkPW[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= NFHYU4ozOjh|N{[4PS=>
K562/Adr  MlHMRZBweHSxc3nzJGF{e2G7 M1HqW|YxKM7:TR?= M4foZ|EzKGh? M3roeIVvcGGwY3XzJJNpcWuxbnnuMYlv\HWlZXSgZZBweHSxc3nz M1zYTVIzQDN5Nki5
HL60 M4T0fGZ2dmO2aX;uJGF{e2G7 NV3vdoJjPjBizszN M2TQTVEzKGh? NVLZeIxE[XWpbXXueJMhfGinIHPhd5Bie2VvMzDhZ5Rqfmm2eR?= NX7xNWFrOjJ6M{e2PFk>
HL60/Adr MknXSpVv[3Srb36gRZN{[Xl? NVjZPG1[PjBizszN M4LGRVEzKGh? NEiwSFJifWevZX70d{B1cGViY3HzdIF{\S1|IHHjeIl3cXS7 NGHY[oEzOjh|N{[4PS=>
K562 MYPGeY5kfGmxbjDBd5NigQ>? MVO2NEDPxE1? NFz6dY8yOiCq MUfheYdu\W62czD0bIUh[2G|cHHz[U0{KGGldHn2bZR6 MkD3NlI5Ozd4OEm=
K562/Adr  NF\CRoxHfW6ldHnvckBCe3OjeR?= NUPwOpJ1PjBizszN M2e1PFEzKGh? Ml7rZZVodWWwdIOgeIhmKGOjc4Dhd4UuOyCjY4Tpeol1gQ>? NWDuTIVZOjJ6M{e2PFk>
HL60 NFjPWGZHfW6ldHnvckBCe3OjeR?= MXS2NEDPxE1? NW\1cGw3OTJiaB?= M3W2OIlv[3KnYYPld{B1cGViYXP0bZZqfHlib3[gZ4F{eGG|ZYOsJINie3Cjc3WgPEBidmRiOR?= MYiyNlg{PzZ6OR?=
HL60/Adr NV;iPXpTTnWwY4Tpc44hSXO|YYm= MlzHOlAh|ryP MYOxNkBp NGD1O|dqdmO{ZXHz[ZMhfGinIHHjeIl3cXS7IH;mJINie3Cjc3XzMEBk[XOyYYPlJFgh[W6mIEm= NGnoUXEzOjh|N{[4PS=>
K562 NYLnUVFFTnWwY4Tpc44hSXO|YYm= NYXEbpptPjBizszN NX2xc2I3OTJiaB?= M3TtZYlv[3KnYYPld{B1cGViYXP0bZZqfHlib3[gZ4F{eGG|ZYOsJINie3Cjc3WgPEBidmRiOR?= M1TRXFIzQDN5Nki5
K562/Adr  MkLqSpVv[3Srb36gRZN{[Xl? MXu2NEDPxE1? MV6xNkBp NFXEXGRqdmO{ZXHz[ZMhfGinIHHjeIl3cXS7IH;mJINie3Cjc3XzMEBk[XOyYYPlJFgh[W6mIEm= MVeyNlg{PzZ6OR?=
L929sA NHTMSldCeG:ydH;zbZMhSXO|YYm= NVjrRmtiOTBizszN MlzNNUBp NV;5bnY6cW6qaXLpeJMhfGinIHHwc5B1d3SrYzDy[ZNxd26|ZTD0c{BVVkZ? MmnuNlI{PjJ5Nke=
L929sA Ml\FRZBweHSxc3nzJGF{e2G7 MkfnNVAh|ryP NWPiRZZIOSCq M{fZNpJme2O3ZYOgZ4VtdHNiZYjwdoV{e2mwZzDSTXBMOc7WSVSg[pJwdSCWTl[tbY5lfWOnZDDhdI9xfG:|aYO= MWiyNlM3Ojd4Nx?=
L929sA NHfI[|FCeG:ydH;zbZMhSXO|YYm= MofYNVAh|ryP MWqxJIg> NX;uZpRO[WK{b3fheIV{KHSqZTDpcpRmemGldHnvckBw\iClYYPwZZNmNThid3n0bEBHSUSG M2rkSlIzOzZ{N{[3
TPC-1 NWS1N3JNS2WubDDWbYFjcWyrdImgRZN{[Xl? MWixNFAh|ryP MXGyOEBp MlrOSG1UVw>? MVnpcoNz\WG|ZYOgZ4VtdHWuYYKgd5Vzfmm4YXy= MUKyNlE{PjhzOB?=
8505c NXz5UoRMS2WubDDWbYFjcWyrdImgRZN{[Xl? MVmxNFAh|ryP NXexXpBFOjRiaB?= MULEUXNQ Ml;ibY5kemWjc3XzJINmdGy3bHHyJJN2en[rdnHs NFjsRnYzOjF|NkixPC=>
SW13 MlTMR4VtdCCYaXHibYxqfHliQYPzZZk> M3zq[|ExOCEQvF2= M1fM[|I1KGh? NWDFfoJXTE2VTx?= MYXpcoNz\WG|ZYOgZ4VtdHWuYYKgd5Vzfmm4YXy= MVeyNlE{PjhzOB?=
Jurkat  Mn3JR5l1d3SxeHnjbZR6KEG|c3H5 NXP3PXBXPTBxIEGwNE8zODEEoN88cS=> NWqzZWRbOS9|IHi= M2XrOZJm\HWlZYRCpG5i\WeuZYLpZUBnd3euZYLpMYlv\HWlZXSgZ5l1d3SxeHnjbZR6 NHnrUHAzOTV|NUCyNC=>
Jurkat  MYXGeY5kfGmxbjDBd5NigQ>? M3\zeVIxOMLizszt MlS4N|AhdWmw MmTodoVlfWOnc9MgUoFm\2yncnnhJIZwf2yncnmtbY5lfWOnZDDy[YFkfGm4ZTDvfJlo\W5ic4DlZ4lmeyBqUl;TLUBo\W6ncnH0bY9v Mk\6NlE2OzVyMkC=
HT-22 NXPhc|dQS3m2b4TvfIlkcXS7IFHzd4F6 M1\XRVExKM7:TR?= MVqxNkBp NFfQTldxem:2ZXP0d{Bi\2GrboP0JINmdGxiZHXheIghcW6mdXPl[EBjgSB3wrDtcY9tN0xiZ3z1eIFu[XSnwrC= NWHPVIJCOTd5NkC4Olk>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Immunofluorescence
RIP1 / RIP3 ; 

PubMed: 30462730     


Immunofluorscence data showed an increased expression of RIP1 and RIP3 in Pg-LPS group and an eminent reduction of RIP1 in the Pg-LPS+Nec-1 group.

30462730

Protocol

Kinase Assay:

[1]

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RIP1 kinase assay:

Phosphorylation of RIP1 requires its kinase activity. Expression constructs of FLAGtagged wild-type (WT) or a kinase-inactive pointmutant of RIP1 (K45M) are are transfected into 293T cells and RIP1 kinase assay is performed as described in the Methods in the presence of [γ-32P]ATP for 30 min at 30℃. Samples are subjected to SDS-PAGE and RIP1 band is visualized by autoradiography. Relative intensities of radioactive bands are quantified and are shown (ratio) in this and all other autoradiographs. In parallel to kinase reactions, a sample of beads is subjected to western blot analysis using anti-RIP1 antibody to ensure equal protein amounts in kinase reactions.
Cell Research:

[2]

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  • Cell lines: Jurkat, BALB/c 3T3, SV40-transformed MEF, L929
  • Concentrations: 0.01-100 μM
  • Incubation Time: --
  • Method:

    Cells are seeded in 96-well plates (white plates for luminescent assays; black plates for fluorescent assays; clear plates for MTT assay) at the density of 5,000-10,000 cells per well for adherent cells or 20,000-50,000 cells per well for suspension cells in 100 μl of the appropriate phenol red-free media. After incubation, we determined cell viability using one of the following methods. For the ATP assay, we used luminescence-based commercial kits and analyzed luminescence using a Wallac Victor II plate reader. For Sytox assay, we incubated cells with 1 μM Sytox Green reagent for 30 min at 37℃, and then performed fluorescent reading. Subsequently, we added 5 μl of 20% Triton X-100 solution into each well to produce maximal lysis and incubated cells for 1 h at 37℃, then performed the second reading. We calculated the ratio of values before and after Triton treatment and normalized it to the relevant controls not subjected to cytotoxic stimuli, as indicated in figure legends. For the MTT assay, we used the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit. For PI exclusion assays, we added 2 μg/ml PI into the medium and immediately analyzed samples using FACSCalibur. For PI-annexin V assay we used the ApoAlert Annexin V-EGFP Apoptosis Kit. For DioC6 staining, we incubated cells with 40 nM DiOC6 for 30 min at 37 ℃, washed once and analyzed in FACSCalibur. For ROS analysis, we incubated cells with 5 μM dihydroethidium for 30 min at 37 ℃, washed once and analyzed in FACSCalibur. EM analyses are performed at the Harvard Medical School EM facility. We acquired bright-field images of the cells using an Axiovert 200 microscope.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 51 mg/mL warmed (196.66 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+45% PEG 300+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 259.33
Formula

C13H13N3OS

CAS No. 4311-88-0
Storage powder
in solvent
Synonyms N/A
Smiles CN1C(=O)C(CC2=C[NH]C3=CC=CC=C23)NC1=S

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID