Necrostatin-1

For research use only.

Catalog No.S8037

80 publications

Necrostatin-1 Chemical Structure

CAS No. 4311-88-0

Necrostatin-1 is a specific RIP1 (RIPK1) inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells. Necrostatin-1 also blocks IDO and suppresses autophagy and apoptosis.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 130 In stock
USD 97 In stock
USD 270 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Necrostatin-1 has been cited by 80 publications

Purity & Quality Control

Choose Selective RIP kinase Inhibitors

Biological Activity

Description Necrostatin-1 is a specific RIP1 (RIPK1) inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells. Necrostatin-1 also blocks IDO and suppresses autophagy and apoptosis.
Features A powerful tool for characterizing the role of necroptosis with characterized primary target.
Targets
RIP1 [1]
(293T cells)
490 nM(EC50)
In vitro

Necrostatin-1 (1-100 μM) inhibits the autophosphorylation of overexpressed and endogenous RIP1.It is found RIP1 is the primary cellular target responsible for the antinecroptosis activity of Necrostatin-1. [1] Necrostatin-1 efficiently suppresses necroptotic cell death triggered by an array of stimuli in a variety of cell types. Necrostatin-1, previously identified as small-molecule inhibitor of necroptosis, inhibits RIP kinase-induced necroptosis and inhibits TNF-α-induced necroptosis in jurkat cells with EC50 of 490 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT-22 MlfsR5l1d3SxeHnjbZR6KEG|c3H5 MnXBNVAh|ryP NEn2c3kyOiCq M4XrTJBzd3SnY4TzJIFo[Wmwc4SgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDYEoH3tc4wwVCCpbIX0ZY1ifGYEoB?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd4MEi2PUc,OTd5NkC4Olk9N2F-
Jurkat  NEPEcY5HfW6ldHnvckBCe3OjeR?= MYqyNFDDqM7:bR?= MXezNEBucW5? NEf0OIxz\WS3Y3XzxsBP[WWpbHXybYEh\m:5bHXybU1qdmS3Y3XkJJJm[WO2aY\lJI95gWenbjDzdIVkcWW|IDjSU3MqKGenbnXyZZRqd25? NF7KXZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWzOVAzOCd-MkG1N|UxOjB:L3G+
Jurkat  MYjDfZRwfG:6aXPpeJkhSXO|YYm= NWfZUVZnPTBxIEGwNE8zODEEoN88cS=> MY[xM|MhcA>? NWj2PYlvemWmdXPld:KhVmGnZ3zldoliKG[xd3zldokucW6mdXPl[EBkgXSxdH;4bYNqfHl? MmrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3M{WwNlAoRjJzNUO1NFIxRC:jPh?=
SW13 M2D6RmNmdGxiVnnhZoltcXS7IFHzd4F6 MonENVAxKM7:TR?= NIXzeGMzPCCq M3H0cmROW09? MoHHbY5kemWjc3XzJINmdGy3bHHyJJN2en[rdnHs MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF|NkixPEc,OjJzM{[4NVg9N2F-
8505c MYfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGTBTogyODBizszN NYfXd4N1OjRiaB?= NYjVR4NUTE2VTx?= MVHpcoNz\WG|ZYOgZ4VtdHWuYYKgd5Vzfmm4YXy= Mn\SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzM{[4NVgoRjJ{MUO2PFE5RC:jPh?=
TPC-1 NVrKeI5iS2WubDDWbYFjcWyrdImgRZN{[Xl? NUHMcXg3OTByIN88US=> M33Rb|I1KGh? NEfOcFBFVVOR MYHpcoNz\WG|ZYOgZ4VtdHWuYYKgd5Vzfmm4YXy= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF|NkixPEc,OjJzM{[4NVg9N2F-
L929sA M2j3RmFxd3C2b4Ppd{BCe3OjeR?= NHTHWngyOCEQvF2= NHqzblIyKGh? MWLhZpJw\2G2ZYOgeIhmKGmwdHXyZYN1cW:wIH;mJINie3Cjc3WtPEB4cXSqIF\BSGQ> Mn7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NkK3OlcoRjJ{M{[yO|Y4RC:jPh?=
L929sA Ml\JRZBweHSxc3nzJGF{e2G7 NFe0PYwyOCEQvF2= MYSxJIg> Mne1doV{[3WnczDj[YxteyCneIDy[ZN{cW6pIGLJVGsy|pSLRDDmdo9uKFSQRj3pcoR2[2WmIHHwc5B1d3Orcx?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN4Mke2O{c,OjJ|NkK3Olc9N2F-
L929sA MnLLRZBweHSxc3nzJGF{e2G7 MWqxNEDPxE1? NIP0c|QyKGh? M{PndolvcGmkaYTzJJRp\SCjcH;weI91cWNicnXzdI9ve2VidH:gWG5H NV\n[I1NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOlI4PjdpPkKyN|YzPzZ5PD;hQi=>
K562/Adr  M{jvS2Z2dmO2aX;uJGF{e2G7 NG[wU3E3OCEQvF2= M2W2T|EzKGh? M33IXIlv[3KnYYPld{B1cGViYXP0bZZqfHlib3[gZ4F{eGG|ZYOsJINie3Cjc3WgPEBidmRiOR?= M36x[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
K562 NXS3XYd6TnWwY4Tpc44hSXO|YYm= NF;qb483OCEQvF2= NHrwZXcyOiCq MVLpcoNz\WG|ZYOgeIhmKGGldHn2bZR6KG:oIHPhd5Bie2W|LDDjZZNx[XOnIEigZY5lKDl? M33YSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
HL60/Adr NWHOSnlCTnWwY4Tpc44hSXO|YYm= MnzCOlAh|ryP NUTNWGZLOTJiaB?= NHPFdYVqdmO{ZXHz[ZMhfGinIHHjeIl3cXS7IH;mJINie3Cjc3XzMEBk[XOyYYPlJFgh[W6mIEm= NH3tbJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizO|Y5QSd-MkK4N|c3QDl:L3G+
HL60 NFq1RZpHfW6ldHnvckBCe3OjeR?= NV;6VWw{PjBizszN NFW3V5IyOiCq NH35PZpqdmO{ZXHz[ZMhfGinIHHjeIl3cXS7IH;mJINie3Cjc3XzMEBk[XOyYYPlJFgh[W6mIEm= NX20b5BMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|c3QDlpPkKyPFM4Pjh7PD;hQi=>
K562/Adr  M2L2SmZ2dmO2aX;uJGF{e2G7 NXTJU3U4PjBizszN MU[xNkBp MnTjZZVodWWwdIOgeIhmKGOjc4Dhd4UuOyCjY4Tpeol1gQ>? M1X0XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
K562 NYfzW4ZCTnWwY4Tpc44hSXO|YYm= MWq2NEDPxE1? MUexNkBp NH7WRnlifWevZX70d{B1cGViY3HzdIF{\S1|IHHjeIl3cXS7 MoPWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{e2PFkoRjJ{OEO3Olg6RC:jPh?=
HL60/Adr NHXJT4hHfW6ldHnvckBCe3OjeR?= MnrPOlAh|ryP MnPFNVIhcA>? NYDSZ4lO[XWpbXXueJMhfGinIHPhd5Bie2VvMzDhZ5Rqfmm2eR?= NH7rRo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizO|Y5QSd-MkK4N|c3QDl:L3G+
HL60 NUSw[plXTnWwY4Tpc44hSXO|YYm= NXvFPJlPPjBizszN Ml7GNVIhcA>? MUfheYdu\W62czD0bIUh[2G|cHHz[U0{KGGldHn2bZR6 NHvBcnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkizO|Y5QSd-MkK4N|c3QDl:L3G+
K562/Adr  MVTBdI9xfG:|aYOgRZN{[Xl? NE\1cpA3OCEQvF2= NGXTSJcyOiCq NXXLcll5\W6qYX7j[ZMhe2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZM> M3:5eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
K562 M3z0SWFxd3C2b4Ppd{BCe3OjeR?= NFn5PWE3OCEQvF2= MWGxNkBp MYflcohidmOnczDzbIlsd26rbj3pcoR2[2WmIHHwc5B1d3Orcx?= M2DaXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
HL60/Adr M3TzOmFxd3C2b4Ppd{BCe3OjeR?= NFrQfHQ3OCEQvF2= M33kNFEzKGh? MUXlcohidmOnczDzbIlsd26rbj3pcoR2[2WmIHHwc5B1d3Orcx?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh|N{[4PUc,OjJ6M{e2PFk9N2F-
HL60 NGnUSIZCeG:ydH;zbZMhSXO|YYm= MXW2NEDPxE1? NFzHSGoyOiCq MnPn[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= M2XUdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO3Olg6Lz5{MkizO|Y5QTxxYU6=
SH-EP NEDweJRCeG:ydH;zbZMhSXO|YYm= M4TNPVEx6oDLzszNxsA> NWnkOVdbPzMkgJnoxsA> MkWxbY5pcWKrdIOgTWFRKGmwaHnibZRwei1iYX7kJGxmgGG2dX31cYFjNWmwZIXj[YQh[XCxcITvd4l{ M4L6VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEmwN|IzLz5{Mki5NFMzOjxxYU6=
NIH3T3  MorJSpVv[3Srb36gRZN{[Xl? M1rzNVExNzVyIN88US=> Mn\XNU8{KGh? NFTDT2xidWWuaX;yZZRmeyCWTldOtU1lemm4ZX6gZ49ueGyneDDmc5Ju[XSrb36= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ4MU[3O{c,OjN{NkG2O|c9N2F-
HT-22  MUHGeY5kfGmxbjDBd5NigQ>? MmjaNlXDqM7:TR?= MmezNQKBmzNywrDtbY4> MYLEUXNQ NWfIXZc4cW6qaXLpeJMhTVKNIFHjeIl3[XSrb36gbY5lfWOnZDDifUBodHW2YX3heIU> NEHHbYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OwO|c2Oid-MkOzNFc4PTJ:L3G+
HT-22  NGfCd5dE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYLrWHZKOTEEoN88US=> Ml3iNVLDqGh? M2XHZmROW09? MX;wdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIHPlcIwh\GWjdHi= MlPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MEe3OVIoRjJ|M{C3O|UzRC:jPh?=
KMS-12-BM Mn;xR5l1d3SxeHnjbZR6KEG|c3H5 NYfWPIxJQTBiwsXN MV:xJIg> NXnOWW9k[myxY3vzJGJCYSBzMT23NFgzKGmwZIXj[YQhemGyaXSgZ4VtdCC|d3XscIlv\w>? M4DzclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3NVU1Lz5{M{WyO|E2PDxxYU6=
MM.1S  NGPtT|hEgXSxdH;4bYNqfHliQYPzZZk> M1fDSVkxKML3TR?= M4HudVEhcA>? MW\icI9kc3NiQlHZJFEyNTdyOEKgbY5lfWOnZDDyZZBq\CClZXzsJJN4\WyuaX7n NHrObJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyO|E2PCd-MkO1NlcyPTR:L3G+
ΔN-Karpas 299  MXjDfZRwfG:6aXPpeJkhSXO|YYm= MWiyNOKh|ryP NHzFTZcyPiCq MUPpcohq[mm2czDDSFMxNWmwZIXj[YQh[2WubDDk[YF1cA>? M1zkTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS1PVM5Lz5{M{W0OVk{QDxxYU6=
MEFs NIrWRYpHfW6ldHnvckBCe3OjeR?= NYnrVpVpOjEEoN88US=> NX7zPXVROS9{L{SgbC=> M3frV5N2eHC{ZYPz[ZMhXE6IzsGtbY5lfWOnZDDSTXBMOSCyaH;zdIhwenmuYYTpc44> MmHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5Mke1PFEoRjJ|N{K3OVgyRC:jPh?=
MEFs NFzjZo1EgXSxdH;4bYNqfHliQYPzZZk> M3m1VlIwPi9{MDFOwG0> MkD2NVghcA>? MUjpcohq[mm2czDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGhiaX6gVoVtSSCNTzDNSWZ{ M4m1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{K3OVgyLz5{M{eyO|U5OTxxYU6=
RMS13 MVHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NIDadIU1OOLCid88[{9udMLi M{fx[lI1KGh? NESyWFhz\XOldXXzJGdZOTVvMEewMYlv\HWlZXSgcI9{eyCxZjDj[YxtKH[rYXLpcIl1gQ>? M1jjUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{S0Nlk3Lz5{M{e0OFI6PjxxYU6=
TE671 NWryRYt2S2WubDDWbYFjcWyrdImgRZN{[Xl? NEDw[Vc1OOLCid88[{9udMLi NWPFe5RwOjRiaB?= MlHxdoV{[3WnczDHXFE2NTB5MD3pcoR2[2WmIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJk> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2NEK5Okc,OjN5NESyPVY9N2F-
U87 NGL3RYVHfW6ldHnvckBCe3OjeR?= MVixJI1ud2xxTB?= M4HmUlEvPS1|IHi= Mlnqd5VxeHKnc4Pld{B1cGViZYjwdoV{e2mxbjDv[kBTUVBvMTDjZZV{\WRiYomgd4hqc2:waX6= MlnzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEC0OFEoRjJ|OESwOFQyRC:jPh?=
C6 MnLUSpVv[3Srb36gRZN{[Xl? MmP1NUBudW:uL1y= NX:xfFcxOS53LUOgbC=> MVzzeZBxemW|c3XzJJRp\SCneIDy[ZN{cW:wIH;mJHJKWC1zIHPheZNm\CCkeTDzbIlsd26rbh?= NYHRTFNVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFA1PDFpPkKzPFQxPDRzPD;hQi=>
U87 NU\neWhuS3m2b4TvfIlkcXS7IFHzd4F6 MnHzNUBudW:uL1y= M1H6eFMhcA>? NFjyOIJjdG:la4Ogd4hqc2:waX6gbY5lfWOnZDDu[YNzd3Orcx?= NFvWW4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0NFQ1OSd-MkO4OFA1PDF:L3G+
C6 NWXYXFRCS3m2b4TvfIlkcXS7IFHzd4F6 MnnmNUBudW:uL1y= NXrQOGZvOyCq NEjGVWVjdG:la4Ogd4hqc2:waX6gbY5lfWOnZDDu[YNzd3Orcx?= MmDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEC0OFEoRjJ|OESwOFQyRC:jPh?=
U87 NXe1W41SS2WubDDWbYFjcWyrdImgRZN{[Xl? Ml\sNUBudW:uL1y= NUXDNpdZOyCq MXPheJRmdnWjdHXzJHNpcWuxbnnuJIlv\HWlZXSg[4xqd22jIHPlcIwh\GWjdHi= NVOzZnU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFA1PDFpPkKzPFQxPDRzPD;hQi=>
C6 NHPYVmdE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MWSxJI1ud2xxTB?= M173XFMhcA>? M3LmdoF1fGWwdXH0[ZMhW2ira3;ubY4hcW6mdXPl[EBodGmxbXGgZ4VtdCCmZXH0bC=> NWPqSlVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFA1PDFpPkKzPFQxPDRzPD;hQi=>
L929 MV3GeY5kfGmxbjDBd5NigQ>? NY[5[GhwPcLizsznM41t MVOyOOKhcA>? M1[0VYJtd2OtczD6WmFFKGmwZIXj[YQhdmWlcn;weI9{cXNiYX7kJIF2fG:yaHHnfS=> NV7CZWdkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFE4PjlpPkKzPVQyPzZ7PD;hQi=>
L929 MmfrSpVv[3Srb36gRZN{[Xl? NE\QOGEzyqEQvHevcYzDqA>? MWCyOOKhcA>? NXTHNVE3eHKxbX;0d{Bk[XOyYYPlMVYhMHB{MDmgZYN1cX[rdImgZY5lKHC{b3Phd5Bie2VvNjDjcIVifmGpZR?= MlHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NEG3OlkoRjJ|OUSxO|Y6RC:jPh?=
L929 NGDvUGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUOyM|XDqM7:Zz;tcOKh NGrNfJEzPMLiaB?= NELEdopz\X[ncoPld{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBidmRiY3XscEBl\WG2aDDpcoR2[2WmIHL5JHRPTs7zIHHsc45mKGG|IIflcIwh[XNiVF7G{tHDqCwEoIrWRWQ> NVjYd3BjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFE4PjlpPkKzPVQyPzZ7PD;hQi=>
L929 NInEe2lHfW6ldHnvckBCe3OjeR?= NEHF[20zNzYEoN88[{9udMLi NH;kPWszPMLiaB?= MnLQdoV3\XK|ZXSgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IGTOSu6yKGGub37lJIF{KHenbHygZZMhXE6IzsJCpEvDqHqYQVS= NH\Sc2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0NVc3QSd-MkO5OFE4Pjl:L3G+
NRK-52E  MXvD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVSyNEDPxE1? M3u3[lI1KGh? M3fYd4lvcGmkaYTzJIlv[3KnYYPl[EBFenBzIIDyc5RmcW5iZYjwdoV{e2mxbjDh[pRmeiCWTl[t{tEhW3SrbYXsZZRqd25iYX7kJGFVWCCGZYDs[ZRqd25? NF[0e2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1NVg1PSd-MkSzOVE5PDV:L3G+
NRK-52E  M3;KbGNmdGxiVnnhZoltcXS7IFHzd4F6 MWOyNEDPxE1? M1PkNVI1KGh? NInLNJpqdmO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiVF7GMe6yKFO2aX31cIF1cW:wIHHu[EBCXFBiRHXwcIV1cW:w Mn\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUG4OFUoRjJ2M{WxPFQ2RC:jPh?=
NRK-52E  Mn\oR4VtdCCYaXHibYxqfHliQYPzZZk> M12z[|IxKM7:TR?= M4jQdFI1KGh? NFLHSGhxem:2ZXP0d{Bk\WyuczDmdo9uKGOnbHyg[IVifGhiY3H1d4VlKGK7IHnzZ4hmdWmjIHnubpVzgQ>? NEfrUGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1NVg1PSd-MkSzOVE5PDV:L3G+
AGS M1LSWGNmdGxiVnnhZoltcXS7IFHzd4F6 NXXpbGVXPjEkgJpOwI0> NH;6SnAy6oDLaB?= MlyxdJJmfmWwdIOgd4hqc2:waX6tbY5lfWOnZDDj[YxtKGSnYYTo Mn3aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NkOxPVkoRjJ2NE[zNVk6RC:jPh?=
L-540  MWDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MUO2NQKBkc7:bR?= NHPIR5Uy6oDLaB?= M1W2NpJm\HWlZYOgeIhmKEerdnnuc5N1[XRxU3;yZYZmdmmkLXnu[JVk\WRiY3XscEBl\WG2aB?= NWrRZm55RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1OlE2OTlpPkK0OVYyPTF7PD;hQi=>
L-540  NH6wUFNHfW6ldHnvckBCe3OjeR?= NFPxe2Y3OOLCid88cS=> M{LGelHjiImq NEe5PIFxemW4ZX70d{B1cGVibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBl\XCxbHHybZpifGmxbh?= MoLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NkG1NVkoRjJ2NU[xOVE6RC:jPh?=
L-540  NFm1doZHfW6ldHnvckBCe3OjeR?= M1LzfFYx6oDLzszt MVOx5qCKcA>? NVPNRXZGeHKndnXueJMhfGinIHflcoVz[XSrb36gc4YhWk:V M{PuNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NU[xOVE6Lz5{NEW2NVUyQTxxYU6=
SK-Hep1 MUfGeY5kfGmxbjDBd5NigQ>? NF:yW4w3OOLCid88UeKh NFrtb20yQOLCiXi= MVTicI9kc3QEoN8yMYxieGGlaH;u[U1u\WSrYYTl[EBRSVJiYXPjeY12dGG2aX;uJIFv\CCDSV[geJJidnOub3PheIlwdiC2bzD0bIUh[3m2b4PvcOKh MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|Mk[wNkc,OjR6M{K2NFI9N2F-
SK-Hep1 MVTGeY5kfGmxbjDBd5NigQ>? M4jOdVYx6oDLzszNxsA> M4S5RVE56oDLaB?= MXLpcohq[mm2czFOtk1N[XCjY3jvcoUucW6mdXPl[EBt\WGtYXflJI9nKEiPR1KtNeKh M33LfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOyOlAzLz5{NEizNlYxOjxxYU6=
SK-Hep1 NEnWUnFHfW6ldHnvckBCe3OjeR?= MmXROlDjiIoQvF5CpC=> MXKxPQKBkWh? Mn;aZoxw[2u|wrFOtk1t[XCjY3jvcoUucW6mdXPl[EBud3KyaH;sc4dq[2GuIHPoZY5o\SxiY3XscEBl\WG2aDDhcoQhWElidYD0ZYtm MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|Mk[wNkc,OjR6M{K2NFI9N2F-
OHC MnjNSpVv[3Srb36gRZN{[Xl? MXGzNFDjiIoQvF2= MnPFSG1UVw>? NEn3eVBqdmO{ZXHz[ZMhfGinIH71cYJmeiCxZjDhdI9xfG:2aXOgU2hEeyC5aYToc5V1KGGudHXybY5oKHSqZTDs[ZZmdHNib3[gR2M5KGGodHXyJI5wcXOnIHX4dI9{fXKn NGDpPWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFc{PCd-MkS4O|Q4OzR:L3G+
OHC MlmzSpVv[3Srb36gRZN{[Xl? M2HSUlMxOOLCid88US=> NUDrXlRjTE2VTx?= M{\ye4RqdWmwaYPo[ZMhdm:rc3WtbY5lfWOnZDDBUXBMKGGldHn2ZZRqd25? M4PkTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0O|M1Lz5{NEi3OFc{PDxxYU6=
OHC NHe5[phHfW6ldHnvckBCe3OjeR?= NH7HVlA{ODEkgJpOwG0> NFfpZ5JFVVOR NIPVU5hz\XO3bITzJIlvKGFicnXkeYN1cW:wIH;mJI5wcXOnLXnu[JVk\WRiUlnQNUBidmRiUlnQN{BqdW23bn;mcJVwemW|Y3XuZ4U> MoHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6N{S3N|QoRjJ2OEe0O|M1RC:jPh?=
OHC MXzGeY5kfGmxbjDBd5NigQ>? M32wWFMxOOLCid88US=> M4jVUWROW09? NHnRR4hl\WO{ZXHz[ZMhdm:rc3WtbY5lfWOnZDDze49tdGWwIH71Z4xmccLi MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh5NEezOEc,OjR6N{S3N|Q9N2F-
OHC MUDGeY5kfGmxbjDBd5NigQ>? NV;WUXlUOzBy4pEJ{txO MnvLSG1UVw>? NIDZfGhqdmO{ZXHz[ZMhdm:rc3WtbY5lfWOnZDDjc45l\W6|ZXSgcpVkdGWr NV\DOohIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4O|Q4OzRpPkK0PFc1PzN2PD;hQi=>
Huh7 MY\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXK1NEDDvU1? NWHae|RJOjRxNEigbC=> Mo\lSG1UVw>? NHTnRWdxemW4ZX70d{Bk\WyuIHTlZZRpKG:oIILB[GhEXiClbz3pcoZm[3SnZDDIeYg4KGOnbHzz M3jtOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUezNlQxLz5{NEm3N|I1ODxxYU6=
L929 MoSwR4VtdCCYaXHibYxqfHliQYPzZZk> MU[zNOKh|ryP M3m3S|HDqGh? M{nFd4lvcGmkaYTzJHRPTi4QsT3pcoR2[2WmIHPs[YF3[WenIH;mJHRweG9iSR?= MoDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOUW3OFIoRjJ3MEm1O|QzRC:jPh?=
L929 MWnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mli3N|DDqM7:TR?= M2rnSlHDqGh? Mmn5bY5pcWKrdIOgWG5HNc7zLXnu[JVk\WRibH;zd{Bw\iClZXzsJJZq[WKrbHn0fS=> M3r0fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEm1O|QzLz5{NUC5OVc1OjxxYU6=
L929-A MXvGeY5kfGmxbjDBd5NigQ>? M3;GW|UxyqEQvF5CpC=> MnjkNVIhcA>? NFmzVWZqdmirYnn0d{B1cGViVF7G{tEucW6mdXPl[EBtd3O|IH;mJI1qfG:laH;u[JJq[WxibXXtZpJidmVicHXycYVi[mmuaYT5 NGLycVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO5PFU1OCd-MkWzPVg2PDB:L3G+
L929 M{nE[2Z2dmO2aX;uJGF{e2G7 Mn24OVDDqM7:TdMg NVnxWmVIOTJiaB?= M{nveYlvcGmkaYTzJHRPTs7zLXnu[JVk\WRiQnnkJINt\WG4YXfl NVLDVnZ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzPVg2PDBpPkK1N|k5PTRyPD;hQi=>
L929-N  NIHMellHfW6ldHnvckBCe3OjeR?= MkPBOVDDqM7:TdMg NIH3SVgyOiCq M3jYSIJtd2OtczD0bIUh[2ynYY\h[4Uhd2ZiQ3HzdIF{\S1|IHHu[EBRSVKS M3HsWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{m4OVQxLz5{NUO5PFU1ODxxYU6=
L929-A  MXnGeY5kfGmxbjDBd5NigQ>? NVnwN|l4PTEEoN88UeKh MoHFNVIhcA>? MnG3Zoxw[2u|IITo[UBkdGWjdnHn[UBw\iCFYYPwZZNmNTNiYX7kJHBCWlB? MmXFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OUi1OFAoRjJ3M{m4OVQxRC:jPh?=
L929-N  NYLPWHlzS2WubDDWbYFjcWyrdImgRZN{[Xl? MXK1NOKh|ryPwrC= Mk\SNlQhcA>? M4PWRYJtd2OtczDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGh? M33HXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{m4OVQxLz5{NUO5PFU1ODxxYU6=
L929-A  M3;1WmNmdGxiVnnhZoltcXS7IFHzd4F6 NYrxZWFMPTEEoN88UeKh NEP3U|gzPCCq MWHicI9kc3NiVF7G{tEucW6mdXPl[EBk\WyuIHTlZZRp MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7OEW0NEc,OjV|OUi1OFA9N2F-
KMS-12-PE  MV\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MX:2NEDPxE1? MlniOUBp NUnVRpI5cW6qaXLpeJMhW0iNLXnu[JVk\WRiY3XscEBl\WG2aB?= NVHufnpsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1N|AxQThpPkK1OVMxODl6PD;hQi=>
SGC-7901 MWHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mkn1N|DDqM7:TR?= Mk\ZNeKhcA>? NHuwVmF{fXCycnXzd4V{KG:6YXzpdIxifGmwLX3l[IlifGWmIHPlcIwh\GWjdHi= NILtbpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe2O|A4Pid-MkW3OlcxPzZ:L3G+
BxPC-3 MUHGeY5kfGmxbjDBd5NigQ>? NFvaRmozOCEQvF2= NXjLSoxuOjRiaB?= NITlWmtl\WO{ZXHz[ZMhfGinIHXhdox6KG6nY4LveIlkKGOnbHzz M{X5ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwOlA4Lz5{NkCwNFYxPzxxYU6=
MiaPaCa-2 MnPSSpVv[3Srb36gRZN{[Xl? NXjyT29vOjBizszN MnLuNlQhcA>? NU\GcHly\GWlcnXhd4V{KHSqZTDlZZJtgSCwZXPyc5Rq[yClZXzsdy=> NWDGVnV1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFA3ODdpPkK2NFAxPjB5PD;hQi=>
NCI-H28 M{\TZmNmdGxiVnnhZoltcXS7IFHzd4F6 M{mxflExyqEQvF2= MVyyOOKhcA>? MnG4dJJmfmWwdIOgSGFRTS2rbnT1Z4VlKHKnZIXjeIlwdiCxZjDOR2kuUDJ6IHPlcIwhfmmjYnnsbZR6yqB? MmLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMESxN|goRjJ4MEC0NVM5RC:jPh?=
BMDM  NIS0RoxHfW6ldHnvckBCe3OjeR?= NEnMVGgyOMLizszN MYmzNOKhdWmw MWLwdo91\WO2czDj[YxteyCocn;tJHRCU0lvaX7keYNm\CCORFigdoVt\WG|ZR?= NV[2WVQ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPFE3ODFpPkK2N|gyPjBzPD;hQi=>
MEFs  M2PKc2NmdGxiVnnhZoltcXS7IFHzd4F6 MWCxNQKBkc7:TR?= MVu0PEBp MmXYSG1UVw>? NVzH[3d5cW6qaXLpeJMhgl[DRD3wdo9ud3SnZDDk[YF1cCCxZjDDUm9VOy2mZYDs[ZRm\CCPRV\z NYfKWnRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0N|c4QDlpPkK2OFM4Pzh7PD;hQi=>
A549 M1\oRmNmdGxiVnnhZoltcXS7IFHzd4F6 Ml;pOVDDqM7:TR?= MofmNlTDqGh? MUPpcohq[mm2czDNUXMucW6mdXPl[EBk\WyuIHTlZZRp NELsSFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkS3NlczOyd-Mk[0O|I4OjN:L3G+
Jurkat T MX;O[YNzd3C2b4Ppd{Bie3OjeR?= MVLJcohq[mm2aX;uJI9nKFSQRj3hcJBp[S2rbnT1Z4VlKG6nY4LvdJRwe2m|IHnuJGZCTERvZHXmbYNq\W62IHj1cYFvKEq3cnvheEBVKGOnbHzzMEBGSzVyIE2gNE4xPSEQvF2u MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR4N{C5OEc,OTh2NkewPVQ9N2F-
Jurkat NX7uNXZFTnWwY4Tpc44h[XO|YYm= M3W5TWlvcGmkaYTpc44hd2ZiZX7kc4dmdm:3czDSTXAyKGG3dH;wbI9{eGixconsZZRqd25iaX6gbJVu[W5iSoXyb4F1KGOnbHzzMEBGSzVyIE2gNE4yQDJizszNMi=> NULaOIxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0NFg4OTNpPkG4OFA5PzF|PD;hQi=>
Sf9 NF7XOVBHfW6ldHnvckBie3OjeR?= NIrzXGE{OCCvaX7z Ml21TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCJU2St[pV{\WRiUlnQT|EhMDFidH:gOFk4KHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6|ZXP0JHNnQSClZXzsd{BqdiCycnXz[Y5k\SCxZjCzNnAu\2GvbXGtRXRRKGGodHXyJFMxKG2rboOgZpkh[XW2b4Lh[Ilw\3KjbT3iZZNm\CCZZYP0[ZJvKGKub4SgcYV1cG:mLDDJR|UxKD1iMD6xPFIh|ryPLh?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ6MEK2NUc,Ojh{OECyOlE9N2F-
Jurkat T NYPP[nIzVmWlcn;weI9{cXNiYYPzZZk> MUTF[oZm[3SrdnWgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CCob4KgbY5pcWKrdHnvckBw\iCwZXPyc5B1d3OrczDpckBHSUSGIHTl[olkcWWwdDDKeZJs[XRiVDDj[YxteyC2cnXheIVlKHerdHigWG5HNWGucHjhMEBGSzVyIE2gNE41QSEQvF2u Ml;LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZzNUO4OFAoRjF4MUWzPFQxRC:jPh?=
Jurkat M1TWbG5m[3KxcITvd4l{KGG|c3H5 MkX6TY5pcWKrdHnvckBw\iClZXzseYxieiCwZXPyc5B1d3OrczDpckBVVk[jbIDoZUB1emWjdHXkJGZCTERiZHXmbYNq\W62IHj1cYFvKEq3cnvheEBk\WyuczygSWM2OCB;IECuOFkh|ryPLh?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDRyOEexN{c,OTh2MEi3NVM9N2F-
Jurkat T MknvUoVkem:ydH;zbZMh[XO|YYm= M2nVTVMxKHWP NXzUVGQ2OjRiaILz MXjJcohq[mm2aX;uJI9nKG6nY4LvdJRwe2m|IHnuJHRPTi2jbIDoZU1qdmS3Y3XkJIh2dWGwIFr1dotifCCWIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGG2IEOwJJVOKGGodHXyJFI1KGi{cx?= NVrxe5QzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0OlcxQTRpPkG4OFY4ODl2PD;hQi=>
L929 MYPO[YNzd3C2b4Ppd{Bie3OjeR?= NWKzPG9pOzBidV2= MWqyOEBpenN? MnXSTY5pcWKrdHnvckBw\iCwZXPyc5B1d3OrczDpckB7XkGGLXnu[JVk\WRibX;1d4UhVDl{OTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjdDCzNEB2VSCjZoTldkAzPCCqcoO= MmP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NkewPVQoRjF6NE[3NFk1RC:jPh?=
L929 NEjhe3hP\WO{b4D0c5NqeyCjc4PhfS=> MXGzNEB2VQ>? NVe2Z|NPOjRiaILz NU\XXVJOUW6qaXLpeIlwdiCxZjDu[YNzd3C2b4Ppd{BqdiCWTl[tZYxxcGFvaX7keYNm\CCvb4Xz[UBNQTJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGG2IEOwJJVOKGGodHXyJFI1KGi{cx?= NYnJTphWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0OlcxQTRpPkG4OFY4ODl2PD;hQi=>
3T3 NV3JSFVzS2WubDDk[YF1cCCjc4PhfS=> M4DMW|I1KGi{cx?= NH;yV5FKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5Rq[yClZXzsJIRm[XSqIHnuJI1wfXOnIEPUN{Bk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliQWTQJIJie2WmII\pZYJqdGm2eTDhd5NigSCrbjDwdoV{\W6lZTDv[kBVVk[jbIDoZUBidmRiem\BSE5ndWt? M2mxRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
3T3 NFm5blFE\WyuIHTlZZRpKGG|c3H5 MVWyOEBpenN? MX\Jcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4TpZ{Bk\WyuIHTlZZRpKGmwIH3veZNmKDOWMzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkAzPCCqcoOgZpkhSVSSIHLhd4VlKH[rYXLpcIl1gSCjc4PhfUBqdiCycnXz[Y5k\SCxZjDGZZNNKGGwZDD6WmFFNm[vax?= NFfCPJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
MEF NHu2UFFE\WyuIHTlZZRpKGG|c3H5 M1P6c|E3KGi{cx?= MnLOTY5pcWKrdHnvckBw\iCmZXH0bEBz\WOncITvdkB{cWewYXzpcochdWWmaXH0[YQhdmWlcn;0bYMh[2WubDDk[YF1cCCrbjDTWlQxKHS{YX7z[o9zdWWmIH3veZNmKE2HRjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkAyPiCqcoOgZpkhSVSSIHLhd4VlKH[rYXLpcIl1gSCjc4PhfUBqdiCycnXz[Y5k\SCxZjDUUmZidHCqYTygR2hZKGGwZDD6WmFFNm[vax?= NIr5OYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
IEC18 M2L5V2NmdGxiZHXheIgh[XO|YYm= NGjVZlRKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5Rq[yClZXzsJIRm[XSqIHnuJJJifCCLRVOxPEBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDpckBxemW|ZX7j[UBw\iCWTl\hcJBp[SCjbnSgfnZCTC6obXu= M4ns[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
HL60 M1XPdGNmdGxiZHXheIgh[XO|YYm= MX;Jcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4TpZ{Bk\WyuIHTlZZRpKGmwIHj1cYFvKEiONkCgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliaX6gdJJme2WwY3Wgc4YhXE6IYXzwbIEh[W6mIIrWRWQv\m2t M2X6WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
L929 M{OxPWNmdGxiZHXheIgh[XO|YYm= MlfSNlQhcHK| Mn;ETY5pcWKrdHnvckBw\iCmZXH0bEBz\WOncITvdkB{cWewYXzpcochdWWmaXH0[YQhdmWlcn;0bYMh[2WubDDk[YF1cCCrbjDtc5V{\SCOOUK5JINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCDVGCgZoF{\WRidnnhZoltcXS7IHHzd4F6KGmwIIDy[ZNmdmOnIH;mJHRPTmGucHjh MkjCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
Jurkat NYTGVVY6VmWlcn;zbZMh[XO|YYm= NYfDWG9WUW6qaXLpeIlwdiCxZjDu[YNzd3OrczDpckBpfW2jbjDKeZJs[XRiY3XscJMh[XO|ZYPz[YQh[XNiboXjcIVieiClb37k[Y5{[XSrb36gZpkh[nKrZ3j0JIZq\WymIH3pZ5Jwe2OxcImgbY4heHKnc3XuZ4Uhd2ZiRnHzUEwhS0i[IHHu[EB7XkGGLX\tby=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyOECwPEc,OTZ2MEiwNFg9N2F-
Jurkat Mm\2UoVkem:|aYOgZZN{[Xl? M{LYbmlvcGmkaYTpc44hd2ZibnXjdo9{cXNiaX6gbJVu[W5iSoXyb4F1KGOnbHzzJIF{e2W|c3XkJIF{KG:{Z3Hu[Yxt\SC|d3XscIlv\yCkeTDidolocHRiZnnlcIQhdWmlcn;zZ49xgSCrbjDwdoV{\W6lZTDv[kBH[XOOLDDDTHgh[W6mIIrWRWQu\m2t NUjnTZVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Jurkat MXrO[YNzd3OrczDhd5NigQ>? M4\BcmlvcGmkaYTpc44hd2ZibnXjdo9{cXNiaX6gbJVu[W5iSoXyb4F1KGOnbHzzJIF{e2W|c3XkJIF{KGWjcnz5JIxwe3Nib3[gdIxie22jIH3lcYJz[W6nIHnueIVoemm2eTDifUBjemmpaISg[olmdGRibXnjdo9{[2:yeTDpckBxemW|ZX7j[UBw\iCIYYPMMEBEUFhiYX7kJJpXSURvZn3r M1f0fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
Jurkat NUfDNWNpVmWlcn;zbZMh[XO|YYm= NF7OUpNKdmirYnn0bY9vKG:oIH7lZ5Jwe2m|IHnuJIh2dWGwIFr1dotifCClZXzsd{Bie3Onc4Pl[EBieyCjcIDlZZJidmOnIH;mJJRz[W6|bIXj[Y51KGO7dH;zc4whcW5icILld4Vv[2Vib3[gSoF{VCxiQ1jYJIFv\CC8VlHEMYZucw>? NGS2TIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Jurkat M{\IcG5m[3Kxc3nzJIF{e2G7 MlPwTY5pcWKrdHnvckBw\iCwZXPyc5NqeyCrbjDoeY1idiCMdYLrZZQh[2WubIOg[IVncWOrZX70JIlvKE[DRFSgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDueYNt\WG{IHPvcoRmdnOjdHnvckBjgSCkcnnnbJQh\mmnbHSgcYlkem:|Y3;wfUBqdiCycnXz[Y5k\SCxZjDUUmZidHCqYR?= NI[zTXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Jurkat NIfLV21P\WO{b4Ppd{Bie3OjeR?= MYnJcohq[mm2aX;uJI9nKG6nY4Lvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDk[YZq[2mnboSgbY4hTkGGRDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIH;y[4Fv\WyuZTDze4VtdGmwZzDifUBjemmpaISg[olmdGRibXnjdo9{[2:yeTDpckBxemW|ZX7j[UBw\iCWTl\hcJBp[Q>? NXL6bYRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Jurkat Mn32UoVkem:|aYOgZZN{[Xl? MXPJcohq[mm2aX;uJI9nKG6nY4Lvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDk[YZq[2mnboSgbY4hTkGGRDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHXhdox6KGyxc4Ogc4YheGyjc33hJI1mdWK{YX7lJIlvfGWpcnn0fUBjgSCkcnnnbJQh\mmnbHSgcYlkem:|Y3;wfUBqdiCycnXz[Y5k\SCxZjDUUmZidHCqYR?= MmK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
Jurkat NGn2WVRP\WO{b4Ppd{Bie3OjeR?= NIjGV|BKdmirYnn0bY9vKG:oIH7lZ5Jwe2m|IHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIFxeGWjcnHuZ4Uhd2ZidILhcpNtfWOnboSgZ5l1d3OxbDDpckBxemW|ZX7j[UBw\iCWTl\hcJBp[Q>? NHvXN3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
U937 NHLBVZZE\WyuIHTlZZRpKGG|c3H5 NULGUYoyPDhiaILz MVvJcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIGW5N|ch[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IFHUVEBj[XOnZDD2bYFjcWyrdImgZZN{[XliaX6gdJJme2WwY3Wgc4YhXE6IYXzwbIEh[W6mIIrWRWQu\m2t Mk\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
3T3 NIP4VoFE\WyuIHTlZZRpKGG|c3H5 MYKyOEBpenN? M4PZbmlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hdW:3c3WgN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IEK0JIhzeyCkeTDBWHAh[mG|ZXSgeoli[mmuaYT5JIF{e2G7IHnuJJBz\XOnbnPlJI9nKFSQRnHsdIhiKGGwZDD6WmFFNW[vax?= Mn\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
Jurkat MWHD[YxtKGSnYYToJIF{e2G7 MV6yOEBpenN? MWPJcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBie3Onc4Pl[EBieyCmZXPy[YF{\WRibHX2[Yx{KG:oIGDFMYNwdmq3Z3H0[YQhVEN|LVnJJEhifXSxcHjh[5khdWG{a3XyLUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[EBqdiCycnXz[Y5k\SCxZjDUUmZidHCqYR?= Mln3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
L929 MVTD[YxtKGSnYYToJIF{e2G7 MV2yOEBpenN? M4DCemlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hdW:3c3WgUFkzQSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\WRibHX2[Yx{KG:oIGDFMYNwdmq3Z3H0[YQh[XW2b4DoZYd6KG2jcnvldkBNSzNvSVmgZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3SgbY4heHKnc3XuZ4Uhd2ZiVF7GZYxxcGF? NF:zd5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
3T3 MWrD[YxtKGSnYYToJIF{e2G7 M{TESlI1KGi{cx?= M4Wxd2lvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hdW:3c3WgN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNm\CCuZY\lcJMhd2ZiUFWtZ49vcnWpYYTl[EBifXSxcHjh[5khdWG{a3XyJGxEOy2LSTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZDDpckBxemW|ZX7j[UBw\iCWTl\hcJBp[SCjbnSgfnZCTC2obXu= M2jBfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEC4NFA5Lz5zNkSwPFAxQDxxYU6=
3T3 NELvUXRE\WyuIHTlZZRpKGG|c3H5 Mn7QNlQhcHK| MUPJcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJI1wfXOnIEPUN{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[YQhdGW4ZXzzJI9nKFCHLXPvcop2\2G2ZXSgZZV1d3CqYXf5JI1iemuncjDMR|MuUUliYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhTmG|TDDhcoQhgl[DRD3mcYs> MnXFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
3T3 NITne3NE\WyuIHTlZZRpKGG|c3H5 Mn\zNlQhcHK| M3PsdGlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hdW:3c3WgN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNm\CCuZY\lcJMhd2ZiUFWtZ49vcnWpYYTl[EBifXSxcHjh[5khdWG{a3XyJGxEOy2LSTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZDDpckBxemW|ZX7j[UBw\iC{YYDhcZlkcW5? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyOECwPEc,OTZ2MEiwNFg9N2F-
Jurkat M2jJXGNmdGxiZHXheIgh[XO|YYm= Mn;0OFghcHK| MoG5TY5pcWKrdHnvckBw\iCmZXH0bEBz\WOncITvdkB{cWewYXzpcochdWWmaXH0[YQhdmWlcn;weI91cWNiY3XscEBl\WG2aDDpckBpfW2jbjDKeZJs[XRiY3XscJMh\GWoaXPp[Y51KGmwIF\BSGQh[W6mIHX4dJJme3OrbnegSmtDWDF{LXLhd4VlKGSrbXXybZpifGmxbjDkc41icW5iYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE[DQ2OgbY4heHKnc3XuZ4Uhd2ZiQWCxOVExNCC8VlHEMYZucw>? NIm3PWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Jurkat M{K0W2NmdGxiZHXheIgh[XO|YYm= NIHZVFg1QCCqcoO= M37qPWlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hcHWvYX6gTpVzc2G2IHPlcIx{KGSnZnnjbYVvfCCrbjDGRWRFKGGwZDDlfJBz\XO|aX7nJHJKWCCtaX7hd4Uh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiaX6gdJJme2WwY3Wgc4YhSVBzNUGwMEB7XkGGLX\tby=> NXLySnkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Jurkat M4H0fGNmdGxiZHXheIgh[XO|YYm= M{[xPFQ5KGi{cx?= NYH6[2R{UW6qaXLpeIlwdiCxZjDk[YF1cCC{ZXPldJRweiC|aXfuZYxqdmdibXXkbYF1\WRibnXjdo9xfG:2aXOgZ4VtdCCmZXH0bEBqdiCqdX3hckBLfXKtYYSgZ4VtdHNiZHXmbYNq\W62IHnuJGZCTERiYX7kJIV5eHKnc4PpcochWkmSIFu0OW0hdXW2YX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGmwIIDy[ZNmdmOnIH;mJGFROTVzMDygfnZCTC2obXu= MkKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEiwNFgoRjF4NEC4NFA5RC:jPh?=
Jurkat M1HT[mNmdGxiZHXheIgh[XO|YYm= MXu0PEBpenN? MVTJcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBidmRiZYjwdoV{e2mwZzDSTXAhc2mwYYPlJIRwdWGrbjDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliRlHDV{BqdiCycnXz[Y5k\SCxZjDBVFE2OTBuIIrWRWQu\m2t NXW3V5piRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Jurkat M2PBUWNmdGxiZHXheIgh[XO|YYm= MV[0PEBpenN? MVLJcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBidmRiZYjwdoV{e2mwZzDGT2JROTJvYnHz[YQh\GmvZYLpfoF1cW:wIHTvcYFqdiCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhTkGFUzDpckBxemW|ZX7j[UBw\iCDUEG1NVA> NYjOOmx3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Jurkat MXXD[YxtKGSnYYToJIF{e2G7 MVu0PEBpenN? M4L3UGlvcGmkaYTpc44hd2ZiZHXheIghemWlZYD0c5Ihe2mpbnHsbY5oKG2nZHnheIVlKG6nY4LvdJRwfGmlIHPlcIwh\GWjdHigbY4hcHWvYX6gTpVzc2G2IHPlcIx{KGSnZnnjbYVvfCCrbjDGRWRFKGGwZDDlfJBz\XO|aX7nJHJKWCCtaX7hd4Uh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiaX6gdJJme2WwY3Wgc4YhSVBzNUGw NIXiS489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwPFAxQCd-MU[0NFgxODh:L3G+
Jurkat M2jnSWNmdGxiZHXheIgh[XO|YYm= NF7IUYQ1QCCqcoO= MXzJcohq[mm2aX;uJI9nKGSnYYToJJJm[2WydH;yJJNq\26jbHnu[{Bu\WSrYYTl[EBv\WO{b4D0c5Rq[yClZXzsJIRm[XSqIHnuJIh2dWGwIFr1dotifCClZXzsd{Bl\W[rY3nlcpQhcW5iRlHESEBidmRiZYjwdoV{e2mwZzDSTXAhUzR3TTDteZRidnRiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE[DQ2OgbY4heHKnc3XuZ4Uhd2ZiQWCxOVEx MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyOECwPEc,OTZ2MEiwNFg9N2F-
Jurkat MlnER4VtdCCmZXH0bEBie3OjeR?= M4fYelQ5KGi{cx?= NYntR49bUW6qaXLpeIlwdiCxZjDk[YF1cCC{ZXPldJRweiC|aXfuZYxqdmdibXXkbYF1\WRibnXjdo9xfG:2aXOgZ4VtdCCmZXH0bEBqdiCqdX3hckBLfXKtYYSgZ4VtdHNiZHXmbYNq\W62IHnuJGZCTERiYX7kJIV5eHKnc4PpcochWkmSIHvpcoF{\SCmb33hbY4h[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiaX6gdJJme2WwY3Wgc4YhSVBzNUGw NYn1b4lCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NFgxODhpPkG2OFA5ODB6PD;hQi=>
Sf9 M1PwbGZ2dmO2aX;uJIF{e2G7 NEP2PVRKdmirYnn0bY9vKG:oIHj1cYFvKFKLUEGgT|Q2VSCvdYThcpQh[XW2b4Doc5NxcG:{eXzheIlwdiCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzz MkX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2MEi3NVMoRjF6NEC4O|E{RC:jPh?=
Jurkat MV7DfZRweHKxdHXjeIl3\SCjc4PhfS=> MV:zNEB2VQ>? MXmxJIhz NYnQZW5nS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDGZZNNNWmwZIXj[YQhdmWlcn;weI9{cXNiaX6gbJVu[W5iSoXyb4F1KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGG2IEOwJJVOKGmwY4XiZZRm\CCob4KgNUBpeiCob3zsc5dm\CCkeTDGZZNNKHO2aX31cIF1cW:wIH3lZZN2emWmIHHmeIVzKDJyIHjyd{BjgSCDbHHtZZIh[my3ZTDhd5NigQ>? MkTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzOVcoRjJ7NUSxN|U4RC:jPh?=
Jurkat M2TwXGN6fG:ycn;0[YN1cX[nIHHzd4F6 MYKzNEB2VQ>? MkDyNUBpeg>? NUD0R5JKS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDDTHgucW6mdXPl[EBv\WO{b4D0c5NqeyCrbjDoeY1idiCMdYLrZZQh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[XRiM{CgeW0hcW6ldXLheIVlKG[xcjCxJIhzKG[xbHzve4VlKGK7IFPIXEB{fGmvdXzheIlwdiCkeTDBcIFu[XJiYnz1[UBie3OjeR?= MofIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEGzOVcoRjJ7NUSxN|U4RC:jPh?=
Jurkat NV3aV5JMS3m2b4Dyc5Rm[3SrdnWgZZN{[Xl? NIHMcoo{OCC3TR?= MXWxJIhz NGHaeG1EgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IGqtWmFFNWmwZIXj[YQhdmWlcn;weI9{cXNiaX6gbJVu[W5iSoXyb4F1KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGG2IEOwJJVOKGmwY4XiZZRm\CCob4KgNUBpeiCob3zsc5dm\CCkeTDaMXZCTCC|dHnteYxifGmxbjDifUBCdGGvYYKgZox2\SCjc4PhfS=> M3\4RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|U4Lz5{OUW0NVM2PzxxYU6=
Jurkat NVP5bnA4S3m2b4Dyc5Rm[3SrdnWgZZN{[Xl? NFW1dXo{OCC3TR?= MkXsNUBpeg>? M{fxOWN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiRnHzUE1qdmS3Y3XkJI5m[3KxcITvd4l{KGmwIHj1cYFvKEq3cnvheEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBifCB|MDD1UUBqdmO3YnH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nliRnHzUEB{fGmvdXzheIlwdiCvZXHzeZJm\CCjZoTldkAzOCCqcoOgZpkheGijc3WgZ49vfHKjc4SgcYlkem:|Y3;wfS=> NEPm[FU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0NVM2Pyd-Mkm1OFE{PTd:L3G+
Jurkat M4PxXWN6fG:ycn;0[YN1cX[nIHHzd4F6 NF\lRm0{OCC3TR?= MlW3NUBpeg>? MXnDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCjZ3HpcpN1KEOKWD3pcoR2[2WmIH7lZ5JweHSxc3nzJIlvKGi3bXHuJGp2emujdDDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDheEA{OCC3TTDpcoN2[mG2ZXSg[o9zKDFiaIKg[o9tdG:5ZXSgZpkhS0i[IIP0bY12dGG2aX;uJIJ6KHCqYYPlJINwdnS{YYP0JI1q[3Kxc3PvdJk> M1i0[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUSxN|U4Lz5{OUW0NVM2PzxxYU6=
Jurkat NU\n[WxyS3m2b4Dyc5Rm[3SrdnWgZZN{[Xl? NXToTGw{OzBidV2= M4LVdVEhcHJ? M3fkPGN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiWj3WRWQucW6mdXPl[EBv\WO{b4D0c5NqeyCrbjDoeY1idiCMdYLrZZQh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[XRiM{CgeW0hcW6ldXLheIVlKG[xcjCxJIhzKG[xbHzve4VlKGK7IGqtWmFFKHO2aX31cIF1cW:wIHL5JJBp[XOnIHPvcpRz[XO2IH3pZ5Jwe2OxcIm= NY[4XYhCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFE{PTdpPkK5OVQyOzV5PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Immunofluorescence
RIP1 / RIP3 ; 

PubMed: 30462730     


Immunofluorscence data showed an increased expression of RIP1 and RIP3 in Pg-LPS group and an eminent reduction of RIP1 in the Pg-LPS+Nec-1 group.

30462730

Protocol

Kinase Assay:

[1]

- Collapse

RIP1 kinase assay:

Phosphorylation of RIP1 requires its kinase activity. Expression constructs of FLAGtagged wild-type (WT) or a kinase-inactive pointmutant of RIP1 (K45M) are are transfected into 293T cells and RIP1 kinase assay is performed as described in the Methods in the presence of [γ-32P]ATP for 30 min at 30℃. Samples are subjected to SDS-PAGE and RIP1 band is visualized by autoradiography. Relative intensities of radioactive bands are quantified and are shown (ratio) in this and all other autoradiographs. In parallel to kinase reactions, a sample of beads is subjected to western blot analysis using anti-RIP1 antibody to ensure equal protein amounts in kinase reactions.
Cell Research:

[2]

- Collapse
  • Cell lines: Jurkat, BALB/c 3T3, SV40-transformed MEF, L929
  • Concentrations: 0.01-100 μM
  • Incubation Time: --
  • Method:

    Cells are seeded in 96-well plates (white plates for luminescent assays; black plates for fluorescent assays; clear plates for MTT assay) at the density of 5,000-10,000 cells per well for adherent cells or 20,000-50,000 cells per well for suspension cells in 100 μl of the appropriate phenol red-free media. After incubation, we determined cell viability using one of the following methods. For the ATP assay, we used luminescence-based commercial kits and analyzed luminescence using a Wallac Victor II plate reader. For Sytox assay, we incubated cells with 1 μM Sytox Green reagent for 30 min at 37℃, and then performed fluorescent reading. Subsequently, we added 5 μl of 20% Triton X-100 solution into each well to produce maximal lysis and incubated cells for 1 h at 37℃, then performed the second reading. We calculated the ratio of values before and after Triton treatment and normalized it to the relevant controls not subjected to cytotoxic stimuli, as indicated in figure legends. For the MTT assay, we used the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit. For PI exclusion assays, we added 2 μg/ml PI into the medium and immediately analyzed samples using FACSCalibur. For PI-annexin V assay we used the ApoAlert Annexin V-EGFP Apoptosis Kit. For DioC6 staining, we incubated cells with 40 nM DiOC6 for 30 min at 37 ℃, washed once and analyzed in FACSCalibur. For ROS analysis, we incubated cells with 5 μM dihydroethidium for 30 min at 37 ℃, washed once and analyzed in FACSCalibur. EM analyses are performed at the Harvard Medical School EM facility. We acquired bright-field images of the cells using an Axiovert 200 microscope.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 51 mg/mL warmed (196.66 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+45% PEG 300+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 259.33
Formula

C13H13N3OS

CAS No. 4311-88-0
Storage powder
in solvent
Synonyms N/A
Smiles CN1C(=O)C(NC1=S)CC2=CNC3=CC=CC=C32

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Necrostatin-1 | Necrostatin-1 supplier | purchase Necrostatin-1 | Necrostatin-1 cost | Necrostatin-1 manufacturer | order Necrostatin-1 | Necrostatin-1 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID