Necrostatin-1

Catalog No.S8037

Necrostatin-1 Chemical Structure

Molecular Weight(MW): 259.33

Necrostatin-1 is a specific RIP1 (RIPK1) inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells.

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Cited by 34 Publications

Purity & Quality Control

Choose Selective TNF-alpha Inhibitors

Biological Activity

Description Necrostatin-1 is a specific RIP1 (RIPK1) inhibitor and inhibits TNF-α-induced necroptosis with EC50 of 490 nM in 293T cells.
Features A powerful tool for characterizing the role of necroptosis with characterized primary target.
Targets
RIP1 [1]
(293T cells)
490 nM(EC50)
In vitro

Necrostatin-1 (1-100 μM) inhibits the autophosphorylation of overexpressed and endogenous RIP1.It is found RIP1 is the primary cellular target responsible for the antinecroptosis activity of Necrostatin-1. [1] Necrostatin-1 efficiently suppresses necroptotic cell death triggered by an array of stimuli in a variety of cell types. Necrostatin-1, previously identified as small-molecule inhibitor of necroptosis, inhibits RIP kinase-induced necroptosis and inhibits TNF-α-induced necroptosis in jurkat cells with EC50 of 490 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 MV;D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHTy[IU2OMLizszN M4jTOVI1yqCq NEnCcJJqdmirYnn0d{BOVVNvaX7keYNm\CClZXzsJIRm[XSq NX:1WJRlOjZ2N{K3NlM>
MEFs  M2\nWWNmdGxiVnnhZoltcXS7IFHzd4F6 M{nwV|Ex6oDLzszN NWfVe4IzPDhiaB?= NHXaPZVFVVOR NFrwdplqdmirYnn0d{B7XkGGLYDyc41wfGWmIHTlZZRpKG:oIFPOU3Q{NWSncHzleIVlKE2HRoO= NXHkbpByOjZ2M{e3PFk>
BMDM  NG\YbpBHfW6ldHnvckBCe3OjeR?= NFjjN2YyOMLizszN M1;QT|MxyqCvaX6= Mnj1dJJwfGWldIOgZ4VtdHNiZoLvcUBVSUuLLXnu[JVk\WRiTFTIJJJmdGWjc3W= NEPsdnQzPjN6MU[wNS=>
NCI-H28 M1XzVmNmdGxiVnnhZoltcXS7IFHzd4F6 NWficoUyOTEEoN88US=> MVmyOOKhcA>? Mn7ZdJJmfmWwdIOgSGFRTS2rbnT1Z4VlKHKnZIXjeIlwdiCxZjDOR2kuUDJ6IHPlcIwhfmmjYnnsbZR6yqB? MVGyOlAxPDF|OB?=
MiaPaCa-2 MWnGeY5kfGmxbjDBd5NigQ>? MWmyNEDPxE1? M3PNT|I1KGh? NViyfIdH\GWlcnXhd4V{KHSqZTDlZZJtgSCwZXPyc5Rq[yClZXzsdy=> NXHzbVU2OjZyMEC2NFc>
BxPC-3 NYLZPZB4TnWwY4Tpc44hSXO|YYm= MX[yNEDPxE1? M4nLS|I1KGh? NHzUVlFl\WO{ZXHz[ZMhfGinIHXhdox6KG6nY4LveIlkKGOnbHzz M1ywZVI3ODByNkC3
SGC-7901 NVrPcYlkS2WubDDWbYFjcWyrdImgRZN{[Xl? NGroXoI{OMLizszN NXHEV|lJOcLiaB?= NGPGZo5{fXCycnXzd4V{KG:6YXzpdIxifGmwLX3l[IlifGWmIHPlcIwh\GWjdHi= NGT2NWUzPTd4N{C3Oi=>
KMS-12-PE  MXHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M3vkclYxKM7:TR?= MXW1JIg> NYG4Wo0xcW6qaXLpeJMhW0iNLXnu[JVk\WRiY3XscEBl\WG2aB?= NXTvTXQzOjV3M{CwPVg>
L929-A  MlT6R4VtdCCYaXHibYxqfHliQYPzZZk> Ml\GOVDDqM7:TdMg NH\Y[XAzPCCq M1XxSYJtd2OtczDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGh? M33iblI2Ozl6NUSw
L929-N  M4m5OGNmdGxiVnnhZoltcXS7IFHzd4F6 NInJNlQ2OMLizszNxsA> MVmyOEBp NUO0R49K[myxY3vzJHRPTs7zLXnu[JVk\WRiY3XscEBl\WG2aB?= MUmyOVM6QDV2MB?=
L929-A  MUTGeY5kfGmxbjDBd5NigQ>? NED5ZXc2OMLizszNxsA> NHnYfIcyOiCq MXzicI9kc3NidHjlJINt\WG4YXflJI9nKEOjc4Dhd4UuOyCjbnSgVGFTWA>? NXHIO2VVOjV|OUi1OFA>
L929-N  NEPzdHJHfW6ldHnvckBCe3OjeR?= MUK1NOKh|ryPwrC= M3vkPFEzKGh? MlLnZoxw[2u|IITo[UBkdGWjdnHn[UBw\iCFYYPwZZNmNTNiYX7kJHBCWlB? MUSyOVM6QDV2MB?=
L929 NWX0bFdyTnWwY4Tpc44hSXO|YYm= NHf3eos2OMLizszNxsA> MWSxNkBp MVLpcohq[mm2czDUUmbPuS2rbnT1Z4VlKEKrZDDjcIVifmGpZR?= Mn7lNlU{QTh3NEC=
L929-A M1fhSWZ2dmO2aX;uJGF{e2G7 MkL6OVDDqM7:TdMg MlnnNVIhcA>? NXjFXpdXcW6qaXLpeJMhfGinIGTOSu6yNWmwZIXj[YQhdG:|czDv[kBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIIDldo1m[WKrbHn0fS=> MlnhNlU{QTh3NEC=
L929 M3XCfmNmdGxiVnnhZoltcXS7IFHzd4F6 MnX3N|DDqM7:TR?= MlPXNeKhcA>? M2rvfIlvcGmkaYTzJHRPTi4QsT3pcoR2[2WmIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJk> MVKyOVA6PTd2Mh?=
L929 Mn2yR4VtdCCYaXHibYxqfHliQYPzZZk> Ml;nN|DDqM7:TR?= NYrsbm52OcLiaB?= NFLhbFNqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDjcIVifmGpZTDv[kBVd3CxIFm= MWiyOVA6PTd2Mh?=
Huh7 NHHXZlFE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NHPP[3o2OCEEtV2= MljGNlQwPDhiaB?= MljjSG1UVw>? MnW2dJJmfmWwdIOgZ4VtdCCmZXH0bEBw\iC{QXTIR3Yh[29vaX7m[YN1\WRiSIXoO{Bk\Wyucx?= M2KxV|I1QTd|MkSw
OHC MXXGeY5kfGmxbjDBd5NigQ>? MoXzN|Ax6oDLzszN NXrCNJo2TE2VTx?= MlHPbY5kemWjc3XzJI5wcXOnLXnu[JVk\WRiY3;u[IVve2WmIH71Z4xmcQ>? NWrCfGtyOjR6N{S3N|Q>
OHC M4nnemZ2dmO2aX;uJGF{e2G7 MYGzNFDjiIoQvF2= M13JNWROW09? MYPk[YNz\WG|ZYOgco9qe2VvaX7keYNm\CC|d3;scIVvKG63Y3zlbeKh NF3QPIszPDh5NEezOC=>
OHC NEPyV5dHfW6ldHnvckBCe3OjeR?= NEO4T|E{ODEkgJpOwG0> M3jSR2ROW09? M33N[ZJme3WudIOgbY4h[SC{ZXT1Z5Rqd25ib3[gco9qe2VvaX7keYNm\CCUSWCxJIFv\CCUSWCzJIludXWwb3\seY9z\XOlZX7j[S=> NUnkcZFEOjR6N{S3N|Q>
OHC M13wTGZ2dmO2aX;uJGF{e2G7 MVqzNFDjiIoQvF2= MkXaSG1UVw>? NHi1[WhlcW2rbnnzbIV{KG6xaYPlMYlv\HWlZXSgRW1RUyCjY4TpeoF1cW:w NWXQUZQ{OjR6N{S3N|Q>
OHC MULGeY5kfGmxbjDBd5NigQ>? MoP6N|Ax6oDLzszN M2GzUWROW09? MV3pcoNz\WG|ZYOgeIhmKG63bXLldkBw\iCjcH;weI91cWNiT1jDd{B4cXSqb4X0JIFtfGW{aX7nJJRp\SCuZY\lcJMhd2ZiQ1O4JIFnfGW{IH7vbZNmKGW6cH;zeZJm NF;SU48zPDh5NEezOC=>
SK-Hep1 MUXGeY5kfGmxbjDBd5NigQ>? MmHEOlDjiIoQvF5CpC=> NEX5PVEyQOLCiXi= MlLPZoxw[2u|wrFOtk1t[XCjY3jvcoUucW6mdXPl[EBud3KyaH;sc4dq[2GuIHPoZY5o\SxiY3XscEBl\WG2aDDhcoQhWElidYD0ZYtm M2KzTFI1QDN{NkCy
SK-Hep1 M2KxZmZ2dmO2aX;uJGF{e2G7 NXjyfIJ1PjEkgJpOwG3DqA>? Mnm4NVjjiImq MWHpcohq[mm2czFOtk1N[XCjY3jvcoUucW6mdXPl[EBt\WGtYXflJI9nKEiPR1KtNeKh NEnMRowzPDh|Mk[wNi=>
SK-Hep1 NHzBSlZHfW6ldHnvckBCe3OjeR?= M1v5NlYx6oDLzszNxsA> MUSxPQKBkWh? MXricI9kc3QEoN8yMYxieGGlaH;u[U1u\WSrYYTl[EBRSVJiYXPjeY12dGG2aX;uJIFv\CCDSV[geJJidnOub3PheIlwdiC2bzD0bIUh[3m2b4PvcOKh M17LSFI1QDN{NkCy
L-540  Mk\3SpVv[3Srb36gRZN{[Xl? NY\QTYg5PjEkgJpOwI0> NVj4ZZRJOeLCiXi= M4[yTJBz\X[nboTzJJRp\SCpZX7ldoF1cW:wIH;mJHJQWw>? NYLKXnd[OjR3NkG1NVk>
L-540  M13C[WZ2dmO2aX;uJGF{e2G7 NEX1cHo3OOLCid88cS=> NGOwVIky6oDLaB?= M{H2XJBz\X[nboTzJJRp\SCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJIRmeG:uYYLpfoF1cW:w MnjlNlQ2PjF3MUm=
L-540  MVjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NWnBWWtiPjEkgJpOwI0> NWPoTnFSOeLCiXi= NEHRVoxz\WS3Y3XzJJRp\SCJaY\pco9{fGG2L2PvdoFn\W6rYj3pcoR2[2WmIHPlcIwh\GWjdHi= NX7kR4tnOjR3NkG1NVk>
AGS MUnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2SxRlYx6oDLzszt M2rXW|HjiImq MYTwdoV3\W62czDzbIlsd26rbj3pcoR2[2WmIHPlcIwh\GWjdHi= MVWyOFQ3OzF7OR?=
NRK-52E  MYHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVSyNEDPxE1? M3jQSFI1KGh? MW\wdo91\WO2czDj[YxteyCocn;tJINmdGxiZHXheIgh[2G3c3XkJIJ6KGm|Y3jlcYliKGmwaoXyfS=> MWeyOFM2OTh2NR?=
NRK-52E  MXzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MY[yNEDPxE1? MlnlNlQhcA>? MULpcoNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhXE6ILd8xJHN1cW23bHH0bY9vKGGwZDDBWHAhTGWybHX0bY9v NH;oXWQzPDN3MUi0OS=>
NRK-52E  MX3D[YxtKF[rYXLpcIl1gSCDc4PhfS=> M{TKXFIxKM7:TR?= MVeyOEBp NE\xe3NqdmirYnn0d{BqdmO{ZXHz[YQhTHKyMTDwdo91\WmwIHX4dJJme3Orb36gZYZ1\XJiVF7GMe6yKFO2aX31cIF1cW:wIHHu[EBCXFBiRHXwcIV1cW:w NIjF[ZIzPDN3MUi0OS=>
L929 MonSSpVv[3Srb36gRZN{[Xl? MXSyM|XDqM7:Zz;tcOKh M2LwZlI1yqCq MWTy[ZZmenOnZDD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF7G{tEh[WyxbnWgZZMhf2WubDDhd{BVVkcQsdMgL:Khgl[DRB?= NYPqeHJrOjN7NEG3Olk>
L929 NIPJVm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXzdFYzNzYEoN88[{9udMLi NG\qOIozPMLiaB?= NXnTdpJxemW4ZYLz[ZMhfGinIHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W6mIHPlcIwh\GWjdHigbY5lfWOnZDDifUBVVkcQsTDhcI9v\SCjczD3[YxtKGG|IGTOSu6yyqBtwrD6WmFF NF;SdFkzOzl2MUe2PS=>
L929 MXrGeY5kfGmxbjDBd5NigQ>? MlHlNuKh|rypL33sxsA> MWCyOOKhcA>? MWnwdo9ud3S|IHPhd5Bie2VvNjCodFIxMSCjY4Tpeol1gSCjbnSgdJJw[2G|cHHz[U03KGOuZXH2ZYdm M1HOPVI{QTRzN{[5
L929 NGLFUmZHfW6ldHnvckBCe3OjeR?= NITVR4g2yqEQvHevcYw> NEjTV3AzPMLiaB?= MX\icI9kc3Niem\BSEBqdmS3Y3XkJI5m[3KxcITvd4l{KGGwZDDheZRweGijZ4m= NIXHTpIzOzl2MUe2PS=>
C6 M3TTb2NmdGxiVnnhZoltcXS7IFHzd4F6 MoPFNUBudW:uL1y= NYjLeFhsOyCq MmK4ZZR1\W63YYTld{BUcGmtb37pckBqdmS3Y3XkJIdtcW:vYTDj[YxtKGSnYYTo MmPSNlM5PDB2NEG=
U87 MYfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2DDb|EhdW2xbD;M MlznN{Bp MoHGZZR1\W63YYTld{BUcGmtb37pckBqdmS3Y3XkJIdtcW:vYTDj[YxtKGSnYYTo M1T2cVI{QDRyNESx
C6 NGjnT3lEgXSxdH;4bYNqfHliQYPzZZk> M{La[FEhdW2xbD;M NV7v[5hPOyCq MlKxZoxw[2u|IIPobYtwdmmwIHnu[JVk\WRibnXjdo9{cXN? NFPZV|QzOzh2MES0NS=>
U87 MV\DfZRwfG:6aXPpeJkhSXO|YYm= NV\CW2ZtOSCvbX;sM2w> MWmzJIg> MofTZoxw[2u|IIPobYtwdmmwIHnu[JVk\WRibnXjdo9{cXN? NIfPSY0zOzh2MES0NS=>
C6 NIrOeZJHfW6ldHnvckBCe3OjeR?= NXzGWJlbOSCvbX;sM2w> MVWxMlUuOyCq MUPzeZBxemW|c3XzJJRp\SCneIDy[ZN{cW:wIH;mJHJKWC1zIHPheZNm\CCkeTDzbIlsd26rbh?= MnziNlM5PDB2NEG=
U87 MX;GeY5kfGmxbjDBd5NigQ>? NYHFRW9IOSCvbX;sM2w> NF3ONnEyNjVvMzDo NXzvbpVte3WycILld5NmeyC2aHWg[ZhxemW|c3nvckBw\iCUSWCtNUBk[XW|ZXSgZpkhe2ira3;ubY4> NXzvR3J3OjN6NEC0OFE>
TE671 MojHR4VtdCCYaXHibYxqfHliQYPzZZk> NH\yb3c1OOLCid88[{9udMLi NEnPO4czPCCq MmC4doV{[3WnczDHXFE2NTB5MD3pcoR2[2WmIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJk> MXOyN|c1PDJ7Nh?=
RMS13 MkmwR4VtdCCYaXHibYxqfHliQYPzZZk> NF23T4s1OOLCid88[{9udMLi MXSyOEBp MWfy[ZNkfWW|IFfYNVUuODdyLXnu[JVk\WRibH;zd{Bw\iClZXzsJJZq[WKrbHn0fS=> NXX3RXZPOjN5NESyPVY>
MEFs MoryR5l1d3SxeHnjbZR6KEG|c3H5 Ml75Nk83NzJyIN88US=> NVnSVIxNOThiaB?= MVvpcohq[mm2czDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGhiaX6gVoVtSSCNTzDNSWZ{ MUWyN|czPzV6MR?=
MEFs NITTb3hHfW6ldHnvckBCe3OjeR?= Ml;vNlDDqM7:TR?= MmDHNU8zNzRiaB?= MV\zeZBxemW|c3XzJHRPTs7zLXnu[JVk\WRiUlnQT|EheGixc4Doc5J6dGG2aX;u MX:yN|czPzV6MR?=
ΔN-Karpas 299  NGXTT4NEgXSxdH;4bYNqfHliQYPzZZk> MXeyNOKh|ryP NGrhXpcyPiCq MlvwbY5pcWKrdIOgR2Q{OC2rbnT1Z4VlKGOnbHyg[IVifGh? NYTmboplOjN3NEW5N|g>
MM.1S  M1\VNGN6fG:2b4jpZ4l1gSCDc4PhfS=> NGnJWXg6OCEEtV2= M4H1SlEhcA>? MWricI9kc3NiQlHZJFEyNTdyOEKgbY5lfWOnZDDyZZBq\CClZXzsJJN4\WyuaX7n M2XoRVI{PTJ5MUW0
KMS-12-BM MnfHR5l1d3SxeHnjbZR6KEG|c3H5 M{jZd|kxKML3TR?= MXqxJIg> NHfZbJRjdG:la4OgRmF[KDFzLUewPFIhcW6mdXPl[EBz[XCrZDDj[YxtKHO5ZXzsbY5o NX7odo1pOjN3MkexOVQ>
HT-22  MknyR4VtdCCYaXHibYxqfHliQYPzZZk> M3y3SVExyqEQvF2= M{nWcVEzyqCq MmjsSG1UVw>? MXLwdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIHPlcIwh\GWjdHi= NGOwNYwzOzNyN{e1Ni=>
HT-22  NYfwWHFxTnWwY4Tpc44hSXO|YYm= MVqyOeKh|ryP NFPjTZUx6oDVM{FCpI1qdg>? NEiyXnBFVVOR MX3pcohq[mm2czDFVmshSWO2aY\heIlwdiCrbnT1Z4VlKGK7IHfseZRidWG2ZR?= NIfiS4szOzNyN{e1Ni=>
NIH3T3  MUHGeY5kfGmxbjDBd5NigQ>? MlXCNVAwPTBizszN MUOxM|MhcA>? NWqz[oxN[W2nbHnvdoF1\XNiVF7G{tEu\HKrdnXuJINwdXCuZYig[o9zdWG2aX;u NEfkS3AzOzJ4MU[3Oy=>
SH-EP M3LB[WFxd3C2b4Ppd{BCe3OjeR?= Ml7GNVDjiIoQvF5CpC=> MWq3NwKBkWkEoB?= NFvZZpRqdmirYnn0d{BKSVBiaX7obYJqfG:{LTDhcoQhVGW6YYT1cZVu[WJvaX7keYNm\CCjcH;weI9{cXN? MYeyNlg6ODN{Mh?=
HL60 Mn;2RZBweHSxc3nzJGF{e2G7 M4LSfFYxKM7:TR?= M13QfVEzKGh? M3\h[oVvcGGwY3XzJJNpcWuxbnnuMYlv\HWlZXSgZZBweHSxc3nz MVyyNlg{PzZ6OR?=
HL60/Adr MVfBdI9xfG:|aYOgRZN{[Xl? M3\0SlYxKM7:TR?= M{faRlEzKGh? MkPq[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= M4HOVlIzQDN5Nki5
K562 M3XFNmFxd3C2b4Ppd{BCe3OjeR?= NYf0dpZnPjBizszN M4\mVFEzKGh? NIrtfmhmdmijbnPld{B{cGmtb37pck1qdmS3Y3XkJIFxd3C2b4Ppdy=> MY[yNlg{PzZ6OR?=
K562/Adr  NF24VJdCeG:ydH;zbZMhSXO|YYm= MVe2NEDPxE1? NWW4[25xOTJiaB?= MkP5[Y5p[W6lZYOgd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYO= NXjKTG9YOjJ6M{e2PFk>
HL60 NHTCNFBHfW6ldHnvckBCe3OjeR?= MV22NEDPxE1? M4HZV|EzKGh? NHnRZZdifWevZX70d{B1cGViY3HzdIF{\S1|IHHjeIl3cXS7 MUKyNlg{PzZ6OR?=
HL60/Adr NYXCRXFbTnWwY4Tpc44hSXO|YYm= M1j0UVYxKM7:TR?= NXzFcJFROTJiaB?= MVzheYdu\W62czD0bIUh[2G|cHHz[U0{KGGldHn2bZR6 NV;QeWgzOjJ6M{e2PFk>
K562 NGH5O5pHfW6ldHnvckBCe3OjeR?= NVW1cY9mPjBizszN M3vINFEzKGh? NEHXd3JifWevZX70d{B1cGViY3HzdIF{\S1|IHHjeIl3cXS7 M3jwVVIzQDN5Nki5
K562/Adr  MWjGeY5kfGmxbjDBd5NigQ>? MlXOOlAh|ryP M2PKWFEzKGh? M2rsW4F2\22nboTzJJRp\SClYYPwZZNmNTNiYXP0bZZqfHl? M1\iXFIzQDN5Nki5
HL60 MoHWSpVv[3Srb36gRZN{[Xl? MXe2NEDPxE1? M{jYVVEzKGh? MVvpcoNz\WG|ZYOgeIhmKGGldHn2bZR6KG:oIHPhd5Bie2W|LDDjZZNx[XOnIEigZY5lKDl? MWiyNlg{PzZ6OR?=
HL60/Adr M17qOGZ2dmO2aX;uJGF{e2G7 MVW2NEDPxE1? MV6xNkBp MmfFbY5kemWjc3XzJJRp\SCjY4Tpeol1gSCxZjDjZZNx[XOnczygZ4F{eGG|ZTC4JIFv\CB7 NUnOV2o3OjJ6M{e2PFk>
K562 NX7mXG85TnWwY4Tpc44hSXO|YYm= NEHiWXE3OCEQvF2= M4qxOFEzKGh? Mn;wbY5kemWjc3XzJJRp\SCjY4Tpeol1gSCxZjDjZZNx[XOnczygZ4F{eGG|ZTC4JIFv\CB7 M2D6cFIzQDN5Nki5
K562/Adr  NYDPe3ltTnWwY4Tpc44hSXO|YYm= NFv3Tng3OCEQvF2= MkL0NVIhcA>? NYfLdlZ5cW6lcnXhd4V{KHSqZTDhZ5Rqfmm2eTDv[kBk[XOyYYPld{wh[2G|cHHz[UA5KGGwZDC5 MUWyNlg{PzZ6OR?=
L929sA M3:ybWFxd3C2b4Ppd{BCe3OjeR?= Mm\wNVAh|ryP NUTGdlFGOSCq Ml;abY5pcWKrdIOgeIhmKGGyb4D0c5Rq[yC{ZYPwc45{\SC2bzDUUmY> M2XMdlIzOzZ{N{[3
L929sA MnuxRZBweHSxc3nzJGF{e2G7 MUexNEDPxE1? MVOxJIg> NVLkWXhjemW|Y4Xld{Bk\WyuczDlfJBz\XO|aX7nJHJKWEtzzqTJSEBnem:vIGTOSk1qdmS3Y3XkJIFxd3C2b4Ppdy=> MUmyNlM3Ojd4Nx?=
L929sA M3zU[GFxd3C2b4Ppd{BCe3OjeR?= NHPRXm0yOCEQvF2= NGjDNGkyKGh? MY\hZpJw\2G2ZYOgeIhmKGmwdHXyZYN1cW:wIH;mJINie3Cjc3WtPEB4cXSqIF\BSGQ> M3nuWFIzOzZ{N{[3
TPC-1 M1\SdWNmdGxiVnnhZoltcXS7IFHzd4F6 NYm5R2VTOTByIN88US=> NYThe|J1OjRiaB?= MnXQSG1UVw>? NETGflJqdmO{ZXHz[ZMh[2WubIXsZZIhe3W{dnn2ZYw> NXnpW4lTOjJzM{[4NVg>
8505c MlnIR4VtdCCYaXHibYxqfHliQYPzZZk> MYGxNFAh|ryP NF;sUVAzPCCq NFz5WoRFVVOR M1nKPYlv[3KnYYPld{Bk\WyudXzhdkB{fXK4aY\hcC=> MV6yNlE{PjhzOB?=
SW13 NXHhbYt4S2WubDDWbYFjcWyrdImgRZN{[Xl? NUeyZnFJOTByIN88US=> MnnSNlQhcA>? M4nEWWROW09? NVHYSJF{cW6lcnXhd4V{KGOnbHz1cIFzKHO3co\peoFt MmLlNlIyOzZ6MUi=
Jurkat  NFTlcmVEgXSxdH;4bYNqfHliQYPzZZk> M2[zXlUxNyBzMECvNlAxyqEQvH2= M4fz[lEwOyCq NV:5UnVVemWmdXPld:KhVmGnZ3zldoliKG[xd3zldokucW6mdXPl[EBkgXSxdH;4bYNqfHl? M1;hSlIyPTN3MEKw
Jurkat  NIfBXGdHfW6ldHnvckBCe3OjeR?= NX;2fHlUOjBywrFOwI0> NIjFc2Q{OCCvaX6= MnrEdoVlfWOnc9MgUoFm\2yncnnhJIZwf2yncnmtbY5lfWOnZDDy[YFkfGm4ZTDvfJlo\W5ic4DlZ4lmeyBqUl;TLUBo\W6ncnH0bY9v MWWyNVU{PTB{MB?=
HT-22 NHvRb5pEgXSxdH;4bYNqfHliQYPzZZk> NICyV5EyOCEQvF2= NFTtRWsyOiCq M2nHXpBzd3SnY4TzJIFo[Wmwc4SgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDYEoH3tc4wwVCCpbIX0ZY1ifGYEoB?= NXHZVZF4OTd5NkC4Olk>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Immunofluorescence
RIP1 / RIP3 ; 

PubMed: 30462730     


Immunofluorscence data showed an increased expression of RIP1 and RIP3 in Pg-LPS group and an eminent reduction of RIP1 in the Pg-LPS+Nec-1 group.

30462730

Protocol

Kinase Assay:

[1]

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RIP1 kinase assay:

Phosphorylation of RIP1 requires its kinase activity. Expression constructs of FLAGtagged wild-type (WT) or a kinase-inactive pointmutant of RIP1 (K45M) are are transfected into 293T cells and RIP1 kinase assay is performed as described in the Methods in the presence of [γ-32P]ATP for 30 min at 30℃. Samples are subjected to SDS-PAGE and RIP1 band is visualized by autoradiography. Relative intensities of radioactive bands are quantified and are shown (ratio) in this and all other autoradiographs. In parallel to kinase reactions, a sample of beads is subjected to western blot analysis using anti-RIP1 antibody to ensure equal protein amounts in kinase reactions.
Cell Research:

[2]

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  • Cell lines: Jurkat, BALB/c 3T3, SV40-transformed MEF, L929
  • Concentrations: 0.01-100 μM
  • Incubation Time: --
  • Method:

    Cells are seeded in 96-well plates (white plates for luminescent assays; black plates for fluorescent assays; clear plates for MTT assay) at the density of 5,000-10,000 cells per well for adherent cells or 20,000-50,000 cells per well for suspension cells in 100 μl of the appropriate phenol red-free media. After incubation, we determined cell viability using one of the following methods. For the ATP assay, we used luminescence-based commercial kits and analyzed luminescence using a Wallac Victor II plate reader. For Sytox assay, we incubated cells with 1 μM Sytox Green reagent for 30 min at 37℃, and then performed fluorescent reading. Subsequently, we added 5 μl of 20% Triton X-100 solution into each well to produce maximal lysis and incubated cells for 1 h at 37℃, then performed the second reading. We calculated the ratio of values before and after Triton treatment and normalized it to the relevant controls not subjected to cytotoxic stimuli, as indicated in figure legends. For the MTT assay, we used the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit. For PI exclusion assays, we added 2 μg/ml PI into the medium and immediately analyzed samples using FACSCalibur. For PI-annexin V assay we used the ApoAlert Annexin V-EGFP Apoptosis Kit. For DioC6 staining, we incubated cells with 40 nM DiOC6 for 30 min at 37 ℃, washed once and analyzed in FACSCalibur. For ROS analysis, we incubated cells with 5 μM dihydroethidium for 30 min at 37 ℃, washed once and analyzed in FACSCalibur. EM analyses are performed at the Harvard Medical School EM facility. We acquired bright-field images of the cells using an Axiovert 200 microscope.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 51 mg/mL warmed (196.66 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+45% PEG 300+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 259.33
Formula

C13H13N3OS

CAS No. 4311-88-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID