Dactolisib (BEZ235)

Name: Dactolisib (BEZ235)
Brand: Selleck Chemicals
SKU: S1009
Rating: 5 (388 reviews)

For research use only.

Catalog No.S1009 Synonyms: NVP-BEZ235

388 publications

CAS No. 915019-65-7

Dactolisib (BEZ235, NVP-BEZ235) is a dual ATP-competitive PI3K and mTOR inhibitor for p110α/γ/δ/β and mTOR(p70S6K) with IC50 of 4 nM /5 nM /7 nM /75 nM /6 nM in cell-free assays, respectively. Inhibits ATR with IC50 of 21 nM in 3T3^TopBP1-ER cell. Dactolisib induces autophagy and suppresses HIV-1 replication. Phase 2.

Selleck's Dactolisib (BEZ235) has been cited by 388 publications

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Nature, 2018, 560(7718):372-376

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Nature, 2017, 543(7647):728-732

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Cancer Cell, 2017, 31(3):424-435

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Nat Med, 2015, 10.1038/nm.3855

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Cell, 2012, 13;149(2):307-21.

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Cancer Cell, 2012, 21(2):155-67

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Cell Rep, 2021, 34(11):108870

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Oncogenesis, 2021, 10(1):8

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Commun Biol, 2020, 3(1):593

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bioRxiv, 2020, 2020/9/20.4.7.30734

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Commun Biol, 2020, 3(1):524

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Biomaterials, 2020, 230:119618

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Cell Oncol (Dordr), 2020, 43(2):223-235

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Cell Rep, 2020, 32(3):107933

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Cancer Cell, 2020, 38(6):872-890.e6

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Neoplasia, 2020, 22(10):470-483

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Clin Transl Med, 2020, 9(1):22

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Anim Biotechnol, 2020, 1-13

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Gastroenterology, 2019, 10.1053/j.gastro.2019.11.013

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Nat Chem Biol, 2019, 15(3):232-240

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Cell Rep, 2019, 27(3):820-834

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Cell Death Differ, 2019, 26(9):1670-1687

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Radiology, 2019, 10.1148/radiol.2019190115

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Oncogene, 2019, 38(15):2690-2705

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Cancers, 2019, 11(2)

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Cancers, 2019, 11(5)

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Biomacromolecules, 2019, 20(9):3313-3323

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J Dent Res, 2019, 98(3):304-312

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Biochem Pharmacol, 2019, 169:113640

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Reprod Sci, 2019, 1933719119828067

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Pharm Biol, 2019, 57(1):412-423

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Exp Ther Med, 2019, 18(3):2278-2284

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Assay Drug Dev Technol, 2019, 17(3):140-149

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Nagoya J Med Sci, 2019, 81(1):93-102

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Cancer Biol Med, 2019, 16(1):66-83

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Viruses, 2019, 11(3)

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Mol Ther Nucleic Acids, 2019, 14:287-300

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ACS Pharmacol Transl Sci, 2019,

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EMBO Mol Med, 2018, 10(9)

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J Invest Dermatol, 2018, 138(11):2377-2387

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J Pathol, 2018, 245(1):101-113

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Cancers (Basel), 2018, 10(12)

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Cancers, 2018, 10(2)

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Cell Death Dis, 2018, 9(2):123

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Cell Death Dis, 2018, 9(2):45

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J Cancer, 2018, 9(6):987-997

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Glia, 2018, 66(5):999-1015

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Radiat Oncol, 2018, 13(1):76

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Mol Cancer Ther, 2018, 17(2):347-354

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Virulence, 2018, 9(1):83-98

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Mol Cancer Res, 2018, 16(4):599-609

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Front Oncol, 2018, 8:249

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Antiviral Res, 2018, 153:39-48

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Biochem Pharmacol, 2018, 10.1016/j.bcp.2018.10.011

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J Proteome Res, 2018, 17(1):63-75

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Acta Pharmacol Sin, 2018, 40(2):268-278

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Invest New Drugs, 2018, 10.1007/s10637-018-0698-2

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Int J Hyperthermia, 2018, 34(6):812-823

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Cancer Med, 2018, 7(9):4744-4754

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Oncol Rep, 2018, 39(2):711-720

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PLoS One, 2018, 13(2):e0191890

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Pathol Res Pract, 2018, 214(6):889-895

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Sci Rep, 2018, 8(1):1652

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Oncotarget, 2018, 9(12):10483-10496

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Discov Med, 2018, 25(140):265-275

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Mol Cell, 2017, 65(6):999-1013

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J Hematol Oncol, 2017, 10(1):9

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Cancer Lett, 2017, 388:12-20

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Cancer Lett, 2017, 406:1-11

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Sci Signal, 2017, 10(507)eaam7550

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Cancers (Basel), 2017, 9(9)E125

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J Nutr Biochem, 2017, 41:124-136

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Acta Pharmacol Sin, 2017, 38(3):382-391

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Mol Cell Neurosci, 2017, 82:66-75

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Cell Cycle, 2017, 16(24):2386-2395

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World J Gastroenterol, 2017, 23(42):7551-7562

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Drug Des Devel Ther, 2017, 11:1115-1126

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Biochem Biophys Res Commun, 2017, 491(1):209-216

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Oncotarget, 2017, 8(50):88043-88058

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Proc Natl Acad Sci U S A, 2017, 114(6):E980-E989

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Nano Lett, 2017, 17(11):7160-7168

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Nat Commun, 2016, 7:11971

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Sci Rep, 2016,

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BMC Cancer, 2016, 16:657

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Cancer Med, 2016, 5(10):2953-2964

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Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description

Targets

p110α ^[1] (Cell-free assay)	p110γ ^[1] (Cell-free assay)	mTOR (p70S6K) ^[1] (Cell-free assay)	p110δ ^[1] (Cell-free assay)	ATR ^[8] (3T3^TopBP1-ER cells)	View More
4 nM	5 nM	6 nM	7 nM	21 nM	View More

In vitro

BEZ235 significantly reduces the phosphorylation levels of the mTOR activated kinase p70S6K. BEZ235 results in a reduction of S235/S236P-RPS6 levels with IC50 of 6.5 nM. The activity of BEZ235 against mTOR is determined using a biochemical mTOR K-LISA assay with IC50 of 20.7 nM. BEZ235 shows slightly lower activity against its β paralogue with IC50 of 75 nM. The PI3K/Akt/mTOR pathway is often constitutively activated in human tumor cells. BEZ235 blocks PI3K and mTOR kinase activity by binding to the ATP-binding cleft of these enzymes. Both PTEN-null cell lines PC3M and U87MG show a dose-dependent reduction in cell proliferation when treated with increasing concentrations of BEZ235 with an average GI50 of 10-12 nM. ^[1] BEZ235 is an mTORC1/2 catalytic inhibitor. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
Trypanosoma brucei brucei	NVewbJAzSW62aX3pZ5Jw[mmjbDDBd5NigQ>?		M1T0NFE5KGh?	MVrEUXNQ	NWTOfVRMSW62aYTyfZBidm:\|b33hcEBi[3Srdnn0fUB4cXSqIFXDOVAhd2ZiMD6wNFI2KM7:TR?=	M4jTUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEC1PVQ3Lz5{NEiwOVk1PjxxYU6=
MCF7	NVfpUHZnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2f5elAvODFvMUCwJO69VQ>?	MUe0PEBp	MYPEUXNQ	M1rDNmlEPTB;MD63N{DPxE1?	NU\WWJFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O\|E6ODRpPkKzPFcyQTB2PD;hQi=>
HCT116	M4D6Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3T0WlAvODFvMUCwJO69VQ>?	M3;Je\|Q5KGh?	M3G2U2ROW09?	NYDlWYVjUUN3ME2xMlE3KM7:TR?=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5MUmwOEc,OjN6N{G5NFQ9N2F-
U87MG	M{LMb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NIHNTpIxNjBzLUGwNEDPxE1?	MWe0PEBp	NFv1[5hFVVOR	NULvSm5RUUN3ME2xMlMzKM7:TR?=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5MUmwOEc,OjN6N{G5NFQ9N2F-
HBC4	MlnHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUjObYliOC5yMT2xNFAh\|ryP	NHj0O2Y1QCCq		M2XUSGxw\yCJSUWwQU05NjZzIF2=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
BSY1	NEHGZY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NX;Od5FSOC5yMT2xNFAh\|ryP	M1fLZVQ5KGh?		NHrhV4FNd2diR1m1NF0uQC52ODDN	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
HBC5	NInKdplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MWmwMlAyNTFyMDFOwG0>	NUnXV3djPDhiaB?=		NVq0NnNUVG:pIFfJOVA:NTdwOEegUS=>	NG\m[489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
MCF7	NYDWOWJTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NYTLTZhXOC5yMT2xNFAh\|ryP	NXfWOnF7PDhiaB?=		M3T0WWxw\yCJSUWwQU05NjF4IF2=	M3nwc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
MDA-MB-231	NIPDVXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1zoVlAvODFvMUCwJO69VQ>?	M{L2S\|Q5KGh?		NX7yfXY2VG:pIFfJOVA:NThwMEmgUS=>	NHnTVpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
U251	NFX5SZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3Tr[lAvODFvMUCwJO69VQ>?	MXq0PEBp		MYrMc4chT0l3ME2tO{43PiCP	M2PhS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
SF268	NUXydFhDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUPmfFh5OC5yMT2xNFAh\|ryP	NH;wcHA1QCCq		NWrDUmJ1VG:pIFfJOVA:NTdwOUKgUS=>	MnK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
SF295	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUSzc445OC5yMT2xNFAh\|ryP	MnzkOFghcA>?		MnG0UI9oKEeLNUC9MVgvOzFiTR?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
SF539	MkLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1LHNFAvODFvMUCwJO69VQ>?	NXz3W3dpPDhiaB?=		NHuyblBNd2diR1m1NF0uQC53OTDN	M3PqPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
SNB75	MmjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NWrWUm13OC5yMT2xNFAh\|ryP	NH\QVlE1QCCq		MXTMc4chT0l3ME2tPE42QCCP	MlnvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
SNB78	M4T6PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3K1VlAvODFvMUCwJO69VQ>?	MnnYOFghcA>?		NH3PdZZNd2diR1m1NF0uQC54NTDN	NHzZNoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
HCC2998	NGrqd4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHjLbocxNjBzLUGwNEDPxE1?	NWDJPXl[PDhiaB?=		NUXQVmd{VG:pIFfJOVA:NThwMEWgUS=>	NF7rS4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
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OVCAR3	M2LtXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmLCNE4xOS1zMECg{txO	NFO4PXg1QCCq		M{X2fWxw\yCJSUWwQU05NjF5IF2=	NFTHU409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
OVCAR4	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVWwMlAyNTFyMDFOwG0>	NF7EXFE1QCCq		MmP5UI9oKEeLNUC9MVgvPThiTR?=	NWXKTY9ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
OVCAR5	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoHaNE4xOS1zMECg{txO	MWO0PEBp		MljXUI9oKEeLNUC9MVcvPTdiTR?=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
OVCAR8	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NHvrXZcxNjBzLUGwNEDPxE1?	MkXHOFghcA>?		M{\k[Wxw\yCJSUWwQU04Njh2IF2=	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
SKOV3	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEHVSIwxNjBzLUGwNEDPxE1?	MUK0PEBp		M3jCWGxw\yCJSUWwQU05NjRzIF2=	NVHRU\|IzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
RXF631L	MlHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mnz4NE4xOS1zMECg{txO	NGnuPY81QCCq		MU\Mc4chT0l3ME2tPE4zOSCP	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
ACHN	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NWjPe5ppOC5yMT2xNFAh\|ryP	MmjJOFghcA>?		NVy1Zo5xVG:pIFfJOVA:NTdwOUWgUS=>	NIm2XJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
St-4	NIPVWXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYfUfZdXOC5yMT2xNFAh\|ryP	NX:0dIxKPDhiaB?=		MXfMc4chT0l3ME2tO{41PyCP	NE\lbJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
MKN1	M1zid2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYGwMlAyNTFyMDFOwG0>	NIC3VYY1QCCq		NILme\|NNd2diR1m1NF0uQC52MTDN	MoHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
MKN7	M3PqXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXewMlAyNTFyMDFOwG0>	M2rRTlQ5KGh?		NX3BNFlSVG:pIFfJOVA:NTdwNkigUS=>	M3fYSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
MKN28	Ml61S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHnnWIQxNjBzLUGwNEDPxE1?	NYO2N256PDhiaB?=		MnnwUI9oKEeLNUC9MVcvODdiTR?=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
MKN45	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M17mU\|AvODFvMUCwJO69VQ>?	MWe0PEBp		NVvJSFEyVG:pIFfJOVA:NThwM{SgUS=>	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
MKN74	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWiwMlAyNTFyMDFOwG0>	M2HyWVQ5KGh?		M{Swd2xw\yCJSUWwQU04NjR{IF2=	NYjIfHp[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
DU145	NGPYbYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4e0e\|AvODFvMUCwJO69VQ>?	M4n6OVQ5KGh?		M4rDUGxw\yCJSUWwQU05NjF4IF2=	NH3w[5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
PC3	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1jWbVAvODFvMUCwJO69VQ>?	MVO0PEBp		M2TyTWxw\yCJSUWwQU05NjZ4IF2=	M1LjZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
Trypanosoma brucei brucei	NXH5XXBYTnWwY4Tpc44hSXO\|YYm=	M2\Rc\|IxOCCwTR?=	Ml3CNVIhcA>?	M2nlcWROW09?	MWDE[YNz\WG\|ZTDpckBxcG:\|cHjveIllgWxiaX7vd4l1d2xicHjvd5Bp[XSnIHzleoVt	M2nJN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEC1PVQ3Lz5{NEiwOVk1PjxxYU6=
Trypanosoma brucei brucei	M1qxN2Z2dmO2aX;uJGF{e2G7		MVqxPEBp	MoPISG1UVw>?	NUjTTJk5UW6qaXLpeIlwdiCxZjDy[YNmeHSxcj3t[YRq[XSnZDDlcoRw[3m2b4Ppd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjD0doFve2[ncoLpckB2eHSja3W=	M3LISFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEC1PVQ3Lz5{NEiwOVk1PjxxYU6=
Trypanosoma brucei brucei	MYLGeY5kfGmxbjDBd5NigQ>?	MX6yNFAhdk1?	NWK3VnpGOTJiaB?=	NIPuNJhFVVOR	MoDKSYZn\WO2IH;uJJNxcGmwZ3;tfYxmcW5ibHX2[Yw>	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhyNUm0Okc,OjR6MEW5OFY9N2F-
Trypanosoma brucei brucei	NETvd3pHfW6ldHnvckBCe3OjeR?=	NViydGFtOjByIH7N	NW[2PG0zOTJiaB?=	Mni2SG1UVw>?	M2\4fWVn\mWldDDvckBxcG:\|cHjheIllgWylaH;sbY5mKGyndnXs	MnLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEW5OFYoRjJ2OEC1PVQ3RC:jPh?=
UM-UC-3	MlfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWPORmRNUUN3ME2zNVUheE1?	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
SBC-1	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M1jFdGlEPTB;N{SyJJBO	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MKN1	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3u1V2lEPTB;Mj64JI5O	NWG0PVZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H720	MnG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2r3NGlEPTB;Mz61O{BvVQ>?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
BFTC-909	MmLsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NVvrNY5RUUN3ME2zMlcyKG6P	NUOwSVNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
BPH-1	NEHqO\|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MV3JR\|UxRTRwN{[gcm0>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
PC-3	NGPNWVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NW\jZZAyUUN3ME21MlA6KG6P	NF\PN2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
AN3-CA	NEfwdVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4nFVGlEPTB;NT61OkBvVQ>?	NFji[Xk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
HN	M{nsWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWHhWJNyUUN3ME21Mlk3KG6P	MmH3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
HCC2218	MmrhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mne0TWM2OD14Lk[xJI5O	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
KP-4	MkniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlHFTWM2OD15IH7N	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
LU-139	NWH6R5VXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MoP6TWM2OD15Lke3JI5O	NGm1clY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
ETK-1	NGrPRY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MoThTWM2OD15Lki4JI5O	M3vGNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
Ramos-2G6-4C10	M{PRVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3PSOmlEPTB;OD60PUBvVQ>?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MHH-PREB-1	M{O1fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2HwTmlEPTB;OT6xNkBvVQ>?	NVHLdW95RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
DU-4475	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGnkd2xKSzVyPUmuOFQhdk1?	MnSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
NCI-H1666	NYHLVo1mT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVzGe5pCUUN3ME25MlcyKG6P	MnL1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CAMA-1	MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NY\BVmk2UUN3ME25Mlghdk1?	NIXlSFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
TYK-nu	NUft[XhUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFy3VnBKSzVyPUmuPVQhdk1?	MmXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
MFE-280	M2nQZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3PNXmlEPTB;OT65PUBvVQ>?	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
A549	MoS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NF7q[ZZKSzVyPUGwMlE1KG6P	M4qyUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
769-P	Mmn6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXrvT3NtUUN3ME2xNE41PCCwTR?=	NF7QfVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
L-363	NVLHb491T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFrpOVRKSzVyPUGwMlY6KG6P	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
ES8	M1LLXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXrJR\|UxRTFyLkmgcm0>	MlriQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
PA-1	M4rKUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MljBTWM2OD1zMT64NkBvVQ>?	NVrpcVVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
Mewo	NGnRdVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVPnUVNRUUN3ME2xNU46QSCwTR?=	M3PUTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
HSC-3	NXXQUYdCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1\yZmlEPTB;MUKuNlEhdk1?	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
H4	NXPoRnRkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVy2bVlNUUN3ME2xNk43PyCwTR?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
BB65-RCC	NWjNfYhoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFzMUYhKSzVyPUGyMlczKG6P	NVu5U3hORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H1793	NYrjdXBXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnGyTWM2OD1zMj64NkBvVQ>?	NHraUJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
KARPAS-45	MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3e4O2lEPTB;MUOuOVEhdk1?	NVvZb2poRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
GCT	M3jYe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGrjRZVKSzVyPUGzMlY5KG6P	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
EoL-1	NX\teIJQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NX;vdFhOUUN3ME2xN{46OyCwTR?=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
LB2241-RCC	NFjhTJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MULJR\|UxRTF2LkCxJI5O	NFrsNpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
ES5	NGDIcGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NILyVlBKSzVyPUG0Mlc1KG6P	MoDWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LOXIMVI	MoPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MljNTWM2OD1zND65PUBvVQ>?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
COR-L105	NEX6XWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmXOTWM2OD1zNT6wOEBvVQ>?	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
P12-ICHIKAWA	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHXnVHZKSzVyPUG1MlEhdk1?	NHrsSXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
CHL-1	NEP0ZY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MoDITWM2OD1zNT60OUBvVQ>?	MkLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
VMRC-RCZ	M3rTRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{TJfGlEPTB;MUWuOlMhdk1?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
HH	M4XKdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1vpcWlEPTB;MUWuO{BvVQ>?	M4nROFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NUGC-3	M4rBUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWjJR\|UxRTF4LkO0JI5O	NWPNcm1LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
EGI-1	MnjFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4nLb2lEPTB;MU[uOFMhdk1?	M2\6SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
OS-RC-2	NYPCTZgyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFu3TY5KSzVyPUG2MlQ4KG6P	NWX5R4E3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
MOLT-16	NWnOfppsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2TpfGlEPTB;MUeuNVUhdk1?	NX;ybVUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H209	NGnFSYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYnJR\|UxRTF5LkK5JI5O	NFXFeIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
697	M{OzNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NITieXdKSzVyPUG3MlQ{KG6P	M2TTdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
D-56MG	M2e2dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MUnJR\|UxRTF5Lk[5JI5O	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
VA-ES-BJ	MlK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1P3SGlEPTB;MUeuPFMhdk1?	NG\qUmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
EW-16	Ml\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M332dWlEPTB;MUiuNFQhdk1?	NUHiNY1ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
HGC-27	M{H2R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlvxTWM2OD1zOD6wOkBvVQ>?	MmWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
ES7	NWLrWFFPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1m0VmlEPTB;MUiuNFkhdk1?	NX7HT2R[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
HuH-7	Mki0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFfZR3ZKSzVyPUG4MlQhdk1?	M1zHXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
BCPAP	M2PtWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4e3dmlEPTB;MUiuOlUhdk1?	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
SW1710	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NEizOnFKSzVyPUG4MlczKG6P	NVfjN5dyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
IST-MEL1	NHnkZ3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWG2RVZmUUN3ME2xPE44PyCwTR?=	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
SF295	M4HjT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXqyVlBVUUN3ME2xPE46OyCwTR?=	M3jFR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
RPMI-7951	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVzSU\|M3UUN3ME2xPU4zQCCwTR?=	MnW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
SF126	NV7RW\|lDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3vhXWlEPTB;MUmuOFEhdk1?	NX7V[3NFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
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ONS-76	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M1PKbWlEPTB;MkmuNFUhdk1?	M3\mO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
D-263MG	NX75VIN2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M{\4OmlEPTB;MkmuN\|Uhdk1?	M37wTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
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T98G	NYntSmNvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MVrJR\|UxRTJ7Lki4JI5O	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
GI-ME-N	M3\hT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3XjSGlEPTB;MkmuPVEhdk1?	NVmxe\|J2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
BHT-101	NVvUc3BiT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M{XyU2lEPTB;M{CuNFUhdk1?	NVWzZoY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NOS-1	NF7nOINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYTRd3d1UUN3ME2zNE4yOiCwTR?=	NISzXXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
AsPC-1	NHHqWHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NIjpdWZKSzVyPUOwMlIhdk1?	NW\E[YpSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
QIMR-WIL	NHLGXYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Mo\DTWM2OD1\|MD62JI5O	MnTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
JVM-3	MknBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXLJR\|UxRTNyLkizJI5O	MnTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
KU-19-19	Ml;JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3\XZ2lEPTB;M{GuNVMhdk1?	M3HkN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
OVCAR-8	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnrTTWM2OD1\|MT6yOkBvVQ>?	MnvaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
BEN	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MlHGTWM2OD1\|MT62N{BvVQ>?	NH7kV3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
BE-13	M3i0TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGj0NItKSzVyPUOxMlgzKG6P	NXTGPY1JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
EW-18	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M2XuWGlEPTB;M{GuPFYhdk1?	M2Li[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
ECC10	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFmzXFFKSzVyPUOxMlk2KG6P	NHe4UmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
CTV-1	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXHhZVhCUUN3ME2zNk45OiCwTR?=	NVjRO3h2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
COLO-800	M3XJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWXyc41SUUN3ME2zNk46OiCwTR?=	NWX0XGZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
A2058	M{DlfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MVPJR\|UxRTN\|LkK1JI5O	NF:1Upo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
VM-CUB-1	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M2Hl[WlEPTB;M{OuOFMhdk1?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
Ca9-22	M{DaPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NYLtNG1FUUN3ME2zN{42PSCwTR?=	M{nyflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
COLO-684	NXHBNYlmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MX3JR\|UxRTN\|LkW4JI5O	NWK2V5JzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
RPMI-8226	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Ml3XTWM2OD1\|Mz64NkBvVQ>?	MmjrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
SK-NEP-1	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHyxbXRKSzVyPUO0MlE2KG6P	NGD1bpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
Becker	M3fPZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NF\yZ5VKSzVyPUO0MlIyKG6P	NHTWdZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
NCI-H28	Mn\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFzGfI9KSzVyPUO0MlQ1KG6P	MljRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CAL-120	M1Kw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXnF[5ZlUUN3ME2zOE44OiCwTR?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MDA-MB-157	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmfJTWM2OD1\|NT60NUBvVQ>?	NXfqb4c6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
OVCAR-5	MkXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXLJR\|UxRTN3LkS3JI5O	M1vnT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
LAMA-84	NV3v[nhwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2jseGlEPTB;M{WuPFchdk1?	Mo\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CAKI-1	NEDhb\|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NE\JVWtKSzVyPUO2MlQ{KG6P	M4\6R\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
Daoy	M1Pj[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFPmOVlKSzVyPUO2Mlchdk1?	MoDFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CAL-62	NUX4N2V7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3TK[WlEPTB;M{[uPFYhdk1?	NIrJWmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
8-MG-BA	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NYjPeXk1UUN3ME2zO{4zPSCwTR?=	NUT3Z5FURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
8505C	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXfJR\|UxRTN5LkWxJI5O	Ml61QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LNCaP-Clone-FGC	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFfoTGZKSzVyPUO3MlU6KG6P	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
CAL-39	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUTYW2F[UUN3ME2zO{46KG6P	M1XYUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
MN-60	M4XJRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1fVNGlEPTB;M{euPVYhdk1?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
RKO	NVPMcnUzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3fz[2lEPTB;M{iuN\|Mhdk1?	NXLob2NsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
U251	MkfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXnJR\|UxRTN6LkSzJI5O	M2T5Z\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SJSA-1	MonKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NG[yWGlKSzVyPUO4MlU1KG6P	MlmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CAL-12T	NHzpbpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1Lzc2lEPTB;M{iuOkBvVQ>?	NV\Bfnc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
HCC1419	NIK4bYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFXqXHBKSzVyPUO4MlcyKG6P	Mnu3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CO-314	NGfDRYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVrJR\|UxRTN6Lke0JI5O	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
LB1047-RCC	NX3jS2hMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFLoT2FKSzVyPUO4MlgyKG6P	NHfYfIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
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NCI-H82	NFHjW3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlnoTWM2OD1{LkmwN\|Y6KM7:TR?=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
JVM-2	NILTO\|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVPJR\|UxRTNwMEOxPFgh\|ryP	NUHqPWo1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
SW1116	NXq0WHFkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkTaTWM2OD1\|LkC1OFEyKM7:TR?=	MlLUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
MDA-MB-453	NFHvVJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M33kSmlEPTB;Mz6xNFQ{QSEQvF2=	NV7sSWNZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
Calu-6	M4PvcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NIXW[YxKSzVyPUOuNVk6PyEQvF2=	M1W0cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SHP-77	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mn6xTWM2OD1\|LkWyPEDPxE1?	M4fkcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
Capan-2	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUHJR\|UxRTRwM{K1PFEh\|ryP	M4TlbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SW948	MlPmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUjjSIhQUUN3ME20Mlc1Pzd7IN88US=>	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
HDLM-2	M2XuPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlPvTWM2OD13LkC3O\|I2KM7:TR?=	NGr0UXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
LAN-6	NVHQVoNIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWjJR\|UxRTZwMkG0PVUh\|ryP	NV3rcYxKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
LU-135	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWXDVIlQUUN3ME22MlQ{ODd5IN88US=>	NWPzXmZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
T84	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mn\UTWM2OD14Lkm4OlQh\|ryP	M{\3S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
CAPAN-1	NUfpcY9ET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVm5NFNxUUN3ME23MlYzOjN\|IN88US=>	M1XuWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SW1417	M{PTPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUG3XYVwUUN3ME23MlkyPzNzIN88US=>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
BALL-1	M2CzPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXnSZ3RZUUN3ME24MlUxPDB7IN88US=>	M{f6clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
HT-29	NYjMfZNoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXLJR\|UxRTlwMEe1PVkh\|ryP	NV25VmdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
C3A	Ml;PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2fVd2lEPTB;OT64PFM6PCEQvF2=	M2qze\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NCI-H1993	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFTxXYxKSzVyPUGwMlUxQDFizszN	M{HlcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SK-MEL-1	NYD2Oo9{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXLUUnBjUUN3ME2xNU43Ojh7IN88US=>	MljhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
DU-145	Mn;iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MljsTWM2OD1zND61NFUzKM7:TR?=	NF\QdpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
KP-N-YN	M3rEXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M33Dd2lEPTB;MUSuOVExOyEQvF2=	NUDXdVJZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
CCRF-CEM	M3zqeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MV7JR\|UxRTF2Lke4NFkh\|ryP	NIHsUHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
ZR-75-30	NHLpSnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NIfSZWFKSzVyPUG2MlM{ODNizszN	NFnoOpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
A673	NHr1OphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MXLJR\|UxRTJyLkW1NFch\|ryP	M3yzelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
K-562	NEix[2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NEDQVJpKSzVyPUK4MlQyQDNizszN	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Myc / Vinculin / pT58 Myc; PubMed: 25934076 Cancer Discov Immunoblots documenting MYC and pT58 MYC stability. SW480 cells were treated with 200nM BEZ235 or solvent control for 24h and cycloheximide (50µg/ml) and harvested at the indicated time points. Vinculin was used as loading control. Exposures of MYC and pT58 MYC blots were adjusted to equalize exposure at 0 min (n=3; unless otherwise indicated, n indicates the number of independent biological repeat experiments in the following legends). pAkt / Akt / Skp2 / AR / pS6 / p27; PubMed: 24347472 Prostate BEZ235 decreases Skp2 and increases AR protein expression inC4-2B cells. Cells were treated with BEZ235 at various doses. Cell lysates were subjected to Westernblot analysis with indicated antibodies. p-S6K / S6K / p-Akt / Akt / p-ABL / BCR-ABL; PubMed: 28435223 Drug Des Devel Ther Dose-dependent inhibition effect of BEZ235 on the phosphorylation levels of S6K, Akt (Ser473), and BCR–ABL in CML cells. Results of Western blot. Bcl-2 / Caspase-3 / Cleaved caspase-3 ; PubMed: 28435223 Drug Des Devel Ther K562 (A1) and KBM7R (A2) cells were treated with BEZ235 for 24 h; total protein lysates were immunoblotted with antibodies for Bcl-2, caspase-3, and cleaved caspase-3. Cyclin D1 / Cyclin D2 ; PubMed: 28435223 Drug Des Devel Ther Dose-dependent inhibition effect of BEZ235 on the expression levels of cyclin D1 and cyclin D2 in K562 and KBM7R cells. Western blot results of K562 (A1) and KBM7R (A2) cells. mTOR / p-mTOR / p70S6K / p-p70S6K ; PubMed: 29581778 J Cancer Eca-109 cells was treated with increasing concentration of BEZ235 (0, 100, 200 and 500 nM) for 48 hours. Western blot showed the inhibition effect of PI3K/mTOR pathway was dose dependent. p-EGFR-Tyr1068 / EGFR / p-HER2-Tyr1221 / p-HER2-Tyr1222 / HER2 / p-HER3-Tyr1289; PubMed: 21278786 Oncogene Immunoblots of protein lysates from BT474 and MCF7-HER2 cells treated with either BEZ235 alone (0–500 n) or the combination of BEZ235 (0–500 n) and the tyrosine kinase inhibitor lapatinib (LAP, 500 n) for 24 h.	25934076 24347472 28435223 29581778 21278786
Immunofluorescence	SP1; PubMed: 26336820 Oncotarget Immunofluorescence assessment of SP1 after BEZ235 treatment for 24 hrs in A673, SKNMC cells and in HFF. p-ErbB2(Y1248); PubMed: 24675532 Cell Res Immunofluorescence staining of BT474.LapR cells grown in 3-D culture under different treatment conditions for a week. Scale bar indicates 50 μm. LC3; PubMed: 23603977 Acta Pharmacol Sin (A) SU-2 cells were treated with NVP-BEZ235 (10 nmol/L) for 12 h and then harvested 24 h after treatment with IR (8 Gy), and analyzed for LC3 expression (top) or the formation of autophagolysosomes by using MDC (middle) and LTR (bottom) by immunofluorescence analysis (600×magnification).	26336820 24675532 23603977
Growth inhibition assay	Cell viability (P3, U87); PubMed: 27542970 BMC Cancer IC50 doses of dactolisib for P3 (left) and U87 (right) glioma cells, generated from MTS assay. Cell viability (breast cancer cells); PubMed: 28060760 Oncotarget Cell viability of breast cancer cells treated by either BEZ235 or TSA for 48h was measured by MTT assay. Each cell line was analyzed in triplicate, and a representative experiment is shown. Cell viability (K562, KBM7R); PubMed: 28435223 Drug Des Devel Ther (A) K562 cells were treated with increasing concentrations of BEZ235 for 24, 48, or 72 h and subjected to MTS assay. (B) KBM7R cells were treated with increasing concentrations of BEZ235 for 24, 48, or 72 h and subjected to MTS assay. (C) K562 cells were treated with imatinib (0–10 μM) alone or imatinib and BEZ235 (0.2 μM) for 48 h and subjected to MTS assay. (D) KBM7R cells were treated with imatinib (0–10 μM) alone or imatinib and BEZ235 (0.2 μM) for 48 h and subjected to MTS assay. Mean ± SD. n=3. *P<0.05, compared to the control group. #P<0.05, compared to the group of IM alone.	27542970 28060760 28435223
ELISA	VEGF; PubMed: 19671762 Mol Cancer Ther NVP-BEZ235 attenuated VEGF secretion by at least 40% in U87 cells. The VEGF level in the serum-free medium was measured in triplicate and the experiment repeated at least twice to confirm results.	19671762

In vivo

BEZ235 induces regression of the tumors (69%) without statistically significant effect on body weight gain. Altogether, these preliminary in vivo efficacy results show that BEZ235 causes disease stasis when administered orally as a single agent and can enhance the efficacy of other anticancer agents when used in combination studies. ^[1]

Protocol

Kinase Assay: ^[1]	- Collapse In vitro Protein Kinase, PI3K, and mTOR Assays: PI3Kα, β, and δ proteins are composed of the iSH2 domain of p85 NH₂-terminally fused to the full-length protein p110 protein, with the exception of α that also does not contain the last 20 amino acids. PI3Kγ is produced as full-length protein deleted for its first 144 amino acids. All constructs are fused to a COOH-terminal His tag for convenient purification and then cloned into the pBlue-Bac4.5 (for α, β, and δ isoforms) or pVL1393 (for γ isoform) plasmids. The different vectors are then cotransfected with BaculoGold WT genomic DNA using methods recommended by the vendor for production of the respective recombinant baculoviruses and proteins. BEZ235 are tested for their activity against PI3K using a Kinase-Glo assay. The kinase reaction is done in 384-well black plate. Each well is loaded with 50 μL of test items (in 90% DMSO) and 5 μL reaction buffer containing 10 μg/mL PI substrate (l-α-phosphatidylinositol; Avanti Polar Lipids; prepared in 3% octyl-glucoside) and the PI3K proteins (10, 25, 10, and 150 nM of p110α, p110β, p110δ, and p110γ, respectively) are then added to it. The reaction is started by the addition of 5 μL of 1 μM ATP prepared in the reaction buffer and is incubated for either 60 (for p110α, p110β, and p110δ) or 120 min (for p110γ). It is terminated by the addition of 10 μL Kinase-Glo buffer. The plates are then read in a Synergy 2 reader for luminescence detection.
Cell Research: ^[2]	- Collapse Cell lines: HCT116, DLD-1 and SW480 cells Concentrations: 0-1 μM Incubation Time: 48 hours Method: The human CRC cell lines, HCT116 (PIK3CA mutant; kinase domain at H1047R), DLD-1 (PIK3CA mutant; helical domain at E545K), and SW480 (PIK3CA wild-type) and isogenic DLD-1 PIK3CA mutant as well as wild-type cells are maintained in DMEM with 10% FBS and 1 × Penicillin/Streptomycin. Cells are plated at different initial densities (HCT116: 3 × 10³ cells/well, DLD-1: 5.5 × 10³ cells/well, SW480: 4.5 × 10³ cells/well, DLD-1 PIK3CA mutant: 7 × 10³ cells/well, and DLD-1 PIK3CA wild-type: 9 × 10³ cells/well) to account for differential growth kinetics. After 16 hours, cells are incubated with increasing concentrations of BEZ235, and the drug-containing growth medium is changed every 24 hours. Cell viability is assessed 16 hours after the initial plating and 48 hours after initiation of drug treatment using the colorimetric MTS assay CellTiter 96® AQueous One Solution Cell Proliferation Assay, as per the manufacturer's instructions. Cell viability after drug treatment is normalized to that of untreated cells also grown for 48 hours. For western blot analysis, cells are plated with zero or maximum inhibitory dose (500 nM) BEZ235 for 2, 6, 24, or 48 hours. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Female Harlan athymic nude mice Dosages: 45 mg/kg Administration: p.o. (Only for Reference)

References

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Solubility (25°C)

In vitro	DMF	18 mg/mL warmed (38.33 mM)
	DMSO	0.01 mg/mL (0.02 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): NMP+PEG300 (10+90, v+v) For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Dactolisib (BEZ235) SDF

Molecular Weight	469.55
Formula	C₃₀H₂₃N₅O
CAS No.	915019-65-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NVP-BEZ235
Smiles	CC(C)(C#N)C1=CC=C(C=C1)N2C3=C4C=C(C=CC4=NC=C3N(C2=O)C)C5=CC6=CC=CC=C6N=C5

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

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Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01856101	Terminated	Drug: BEZ235	Carcinoma Transitional Cell	Cliniques universitaires Saint-Luc- Université Catholique de Louvain\|Novartis	February 2013	Phase 2
NCT01717898	Terminated	Drug: BEZ235\|Drug: Prednisone\|Drug: Abiraterone acetate	Castrate-resistant Prostate Cancer	Charles Ryan\|Novartis Pharmaceuticals\|University of California San Francisco	January 31 2013	Phase 1\|Phase 2
NCT01756118	Unknown status	Drug: BEZ235	Acute Lymphoblastic Leukemia\|Leukemia Myelocytic Acute\|Chronic Myelogenous Leukemia With Crisis of Blast Cells	Johann Wolfgang Goethe University Hospital	June 2012	Phase 1
NCT01290406	Withdrawn	Drug: BEZ235	Endometrial Cancer	Novartis Pharmaceuticals\|Novartis	March 2012	Phase 2
NCT01288092	Withdrawn	Drug: BEZ235	Metastatic Breast Cancer	Novartis Pharmaceuticals\|Novartis	March 2012	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
With difficulties in dissolving BEZ235, How can I improve its solubility?
Answer:
For in vivo study, we suggest the vehicle solution NMP/polyethylene glycol 300 (10/90, v/v). Please dissolve BEZ235 in NMP as more as possible. You can sonicate and warm it in water bath for a while. Then dilute with PEG 300.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

Selective PI3K Inhibitors

CAL-101 (Idelalisib, GS-1101) : p110δ-selective, IC50=2.5 nM.
Selective PI3K Inhibitors

TGX-221 : p110β-specific, IC50=5 nM.
Most Potent PI3K Inhibitor

Duvelisib (IPI-145, INK1197) : PI3K δ, IC50=1 nM.
FDA-approved PI3K Inhibitor

CAL-101 (Idelalisib, GS-1101) : Approved by FDA for Relapsed Chronic Lymphocytic Leukemia, Follicular Lymphoma and Small Lymphocytic Lymphoma.
Newest PI3K Inhibitor

GSK2636771 : Potent, orally bioavailable, PI3Kβ-selective inhibitor, sensitive to PTEN null cell lines.

Related PI3K Products

Taselisib (GDC 0032) ^New

Taselisib (GDC 0032, RG7604) is a potent, next-generation β isoform-sparing PI3K inhibitor targeting PI3Kα/δ/γ with K_i of 0.29 nM/0.12 nM/0.97nM, >10 fold selective over PI3Kβ.

Features:A beta isoform-sparing PI3K inhibitor.
Pilaralisib (XL147) ^New

Pilaralisib (XL147) is a selective and reversible class I PI3K inhibitor for PI3Kα/δ/γ with IC50 of 39 nM/36 nM/23 nM in cell-free assays, less potent to PI3Kβ. Phase 1/2.
GNE-317 ^New

GNE-317 is a potent, brain-penetrant PI3K inhibitor.
LY294002

LY294002 (SF 1101, NSC 697286) is the first synthetic molecule known to inhibit PI3Kα/δ/β with IC50 of 0.5 μM/0.57 μM/0.97 μM, respectively; more stable in solution than Wortmannin, and also blocks autophagosome formation. It not only binds to class I PI3Ks and other PI3K-related kinases, but also to novel targets seemingly unrelated to the PI3K family. LY294002 also inhibits CK2 with IC50 of 98 nM. LY294002 is a non-specific DNA-PKcs inhibitor and activates autophagy and apoptosis.
3-Methyladenine (3-MA)

3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.
Idelalisib (CAL-101, GS-1101)

Idelalisib (CAL-101, GS-1101) is a selective p110δ inhibitor with IC50 of 2.5 nM in cell-free assays; shown to have 40- to 300-fold greater selectivity for p110δ than p110α/β/γ, and 400- to 4000-fold more selectivity to p110δ than C2β, hVPS34, DNA-PK and mTOR. Idelalisib also stimulates autophagy.
Wortmannin

Wortmannin (KY 12420, SL-2052) is the first described PI3K inhibitor with IC50 of 3 nM in a cell-free assay, with little selectivity within the PI3K family. Wortmannin blocks autophagosome formation and potently inhibits DNA-PK/ATM with IC50 of 16 nM and 150 nM in cell-free assays. Wortmannin also inhibits PLK1 activity.
Buparlisib (BKM120)

Buparlisib (BKM120, NVP-BKM120) is a selective PI3K inhibitor of p110α/β/δ/γ with IC50 of 52 nM/166 nM/116 nM/262 nM in cell-free assays, respectively. Reduced potency against VPS34, mTOR, DNAPK, with little activity to PI4Kβ. Buparlisib induces apoptosis. Phase 2.
Pictilisib (GDC-0941)

Pictilisib (GDC-0941, RG7321) is a potent inhibitor of PI3Kα/δ with IC50 of 3 nM in cell-free assays, with modest selectivity against p110β (11-fold) and p110γ (25-fold). Pictilisib (GDC-0941) induces autophagy and apoptosis. Phase 2.
Alpelisib (BYL719)

Alpelisib (BYL719) is a potent and selective PI3Kα inhibitor with IC50 of 5 nM in a cell-free assay, and minimal effect on PI3Kβ/γ/δ. Phase 2.

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Dactolisib (BEZ235)