Dactolisib (BEZ235)

Name: Dactolisib (BEZ235)
Brand: Selleck Chemicals
SKU: S1009
Rating: 5 (408 reviews)

For research use only.

Catalog No.S1009 Synonyms: NVP-BEZ235

408 publications

CAS No. 915019-65-7

Dactolisib (BEZ235, NVP-BEZ235) is a dual ATP-competitive PI3K and mTOR inhibitor for p110α/γ/δ/β and mTOR(p70S6K) with IC50 of 4 nM /5 nM /7 nM /75 nM /6 nM in cell-free assays, respectively. Inhibits ATR with IC50 of 21 nM in 3T3^TopBP1-ER cell. Dactolisib induces autophagy and suppresses HIV-1 replication. Phase 2.

Selleck's Dactolisib (BEZ235) has been cited by 408 publications

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Cancer Cell, 2019, 35(5):752-766

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Cancer Cell, 2019, 36(2):179-193

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Nature, 2018, 560(7718):372-376

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Nature, 2017, 543(7647):728-732

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Cancer Cell, 2017, 31(3):424-435

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Nat Med, 2015, 10.1038/nm.3855

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Nature, 2014, 30;514(7524):628-32.

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Nature, 2012, 26;487(7408):505-9.

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Cell, 2012, 13;149(2):307-21.

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Cancer Cell, 2012, 21(2):155-67

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Cell Rep, 2021, 34(11):108870

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J Pathol, 2021, 10.1002/path.5736

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Cell Death Dis, 2021, 12(8):713

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Cell Chem Biol, 2021, S2451-9456(21)00254-3

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BMC Cancer, 2020, 20(1):4

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Oncol Lett, 2020, 20(6):326

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Commun Biol, 2020, 3(1):593

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bioRxiv, 2020, 2020/9/20.4.7.30734

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Commun Biol, 2020, 3(1):524

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Biochem Genet, 2020, 10.1007/s10528-020-09982-x

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Clin Cancer Res, 2020, 10.1158/1078-0432.CCR-19-4245

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Biomaterials, 2020, 230:119618

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Cell Oncol (Dordr), 2020, 43(2):223-235

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Cell Rep, 2020, 32(3):107933

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Cancer Cell, 2020, 38(6):872-890.e6

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Neoplasia, 2020, 22(10):470-483

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Clin Transl Med, 2020, 9(1):22

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Anim Biotechnol, 2020, 1-13

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Gastroenterology, 2019, 10.1053/j.gastro.2019.11.013

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Nat Chem Biol, 2019, 15(3):232-240

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Cancer Res, 2019, 79(1):209-219

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Cell Rep, 2019, 27(3):820-834

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Radiology, 2019, 10.1148/radiol.2019190115

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Oncogene, 2019, 38(15):2690-2705

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Cancers, 2019, 11(2)

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Cancers, 2019, 11(5)

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Biomacromolecules, 2019, 20(9):3313-3323

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Biochem Pharmacol, 2019, 169:113640

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Cancer Cell Int, 2019, 19:336

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J Cell Physiol, 2019, 234(10):18249-18261

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Invest Ophthalmol Vis Sci, 2019, 60(7):2764-2772

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BMC Cancer, 2019, 20(1):1

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Nagoya J Med Sci, 2019, 81(1):93-102

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Cancer Biol Med, 2019, 16(1):66-83

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Viruses, 2019, 11(3)

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Mol Ther Nucleic Acids, 2019, 14:287-300

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ACS Pharmacol Transl Sci, 2019,

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EMBO Mol Med, 2018, 10(9)

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Clin Cancer Res, 2018, 24(20):5165-5177

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Proc Natl Acad Sci USA, 2018,

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Cancer Res, 2018, 78(9):2383-2395

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Cancer Res, 2018, 78(15):4396-4410

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Radiology, 2018, 288(3):730-738

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J Invest Dermatol, 2018, 138(11):2377-2387

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J Pathol, 2018, 245(1):101-113

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Cancers (Basel), 2018, 10(12)

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Cancers, 2018, 10(2)

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Cell Death Dis, 2018, 9(2):123

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Cell Death Dis, 2018, 9(2):45

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J Cancer, 2018, 9(6):987-997

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Glia, 2018, 66(5):999-1015

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Exp Mol Med, 2018, 50(3):e463

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Radiat Oncol, 2018, 13(1):76

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Mol Cancer Ther, 2018, 17(2):347-354

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Virulence, 2018, 9(1):83-98

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Mol Cancer Res, 2018, 16(4):599-609

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Front Oncol, 2018, 8:249

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Antiviral Res, 2018, 153:39-48

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Biochem Pharmacol, 2018, 10.1016/j.bcp.2018.10.011

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J Biol Chem, 2018, 293(16):5808-5820

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J Proteome Res, 2018, 17(1):63-75

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Acta Pharmacol Sin, 2018, 40(2):268-278

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Int J Hyperthermia, 2018, 34(6):812-823

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Oncol Rep, 2018, 39(2):711-720

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PLoS One, 2018, 13(2):e0191890

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Anticancer Res, 2018, 38(3):1407-1414

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Pathol Res Pract, 2018, 214(6):889-895

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Oncol Lett, 2018, 15(6):9655-9662

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Oncotarget, 2018, 9(6):7023-7035

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Sci Rep, 2018, 8(1):1652

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Oncotarget, 2018, 9(12):10483-10496

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Discov Med, 2018, 25(140):265-275

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Mol Cell, 2017, 65(6):999-1013

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Nat Commun, 2017, 8(1):1636

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Clin Cancer Res, 2017, 23(5):1274-1285

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Leukemia, 2017, 31(4):922-933

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Proc Natl Acad Sci U S A, 2017, 114(7):E1072-E1080

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Cell Syst, 2017,

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Clin Cancer Res, 2017, 23(5):1286-1298

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J Hematol Oncol, 2017, 10(1):9

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Cancer Lett, 2017, 388:12-20

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Cancer Lett, 2017, 406:1-11

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Sci Signal, 2017, 10(507)eaam7550

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J Med Chem, 2017,

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Cancers (Basel), 2017, 9(9)E125

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Cell Physiol Biochem, 2017, 43(5):1813-1828

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Mol Cancer Ther, 2017, 16(4):729-738

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Mol Oncol, 2017, 11(9):1130-1142

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J Nutr Biochem, 2017, 41:124-136

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J Cell Mol Med, 2017, 21(3):467-474

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Sci Rep, 2017, 7(1):10612

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Mol Imaging Biol, 2017, 19(3):421-428

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Acta Pharmacol Sin, 2017, 38(3):382-391

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Mol Cell Neurosci, 2017, 82:66-75

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Cell Cycle, 2017, 16(24):2386-2395

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World J Gastroenterol, 2017, 23(42):7551-7562

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Drug Des Devel Ther, 2017, 11:1115-1126

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Biochem Biophys Res Commun, 2017, 491(1):209-216

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SLAS Discov, 2017, 22(5):507-515

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Oncol Lett, 2017, 14(6):7970-7976

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Onco Targets Ther, 2017, 10:5077-5087

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Oncotarget, 2017, 8(11):17995-18009

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Oncotarget, 2017, 8(33):53968-53977

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Oncotarget, 2017, 8(64):107977-107990

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Cell Cycle, 2017, 16(21):2146-2155

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Oncotarget, 2017, 8(50):88043-88058

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Oncotarget, 2017, 8(30):49380-49394

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Oncotarget, 2017, 8(63):106486-106498

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Proc Natl Acad Sci U S A, 2017, 114(6):E980-E989

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Nano Lett, 2017, 17(11):7160-7168

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Oncotarget, 2017, 8(5):7265-7275

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Nat Commun, 2016, 7:11971

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Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description

Targets

p110α ^[1] (Cell-free assay)	p110γ ^[1] (Cell-free assay)	mTOR (p70S6K) ^[1] (Cell-free assay)	p110δ ^[1] (Cell-free assay)	ATR ^[8] (3T3^TopBP1-ER cells)	View More
4 nM	5 nM	6 nM	7 nM	21 nM	View More

In vitro

BEZ235 significantly reduces the phosphorylation levels of the mTOR activated kinase p70S6K. BEZ235 results in a reduction of S235/S236P-RPS6 levels with IC50 of 6.5 nM. The activity of BEZ235 against mTOR is determined using a biochemical mTOR K-LISA assay with IC50 of 20.7 nM. BEZ235 shows slightly lower activity against its β paralogue with IC50 of 75 nM. The PI3K/Akt/mTOR pathway is often constitutively activated in human tumor cells. BEZ235 blocks PI3K and mTOR kinase activity by binding to the ATP-binding cleft of these enzymes. Both PTEN-null cell lines PC3M and U87MG show a dose-dependent reduction in cell proliferation when treated with increasing concentrations of BEZ235 with an average GI50 of 10-12 nM. ^[1] BEZ235 is an mTORC1/2 catalytic inhibitor. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
Trypanosoma brucei brucei	M4G1UmFvfGmvaXPyc4Jq[WxiQYPzZZk>		NYPOPJRZOThiaB?=	Mn7kSG1UVw>?	MorFRY51cXS{eYDhco9{d22jbDDhZ5Rqfmm2eTD3bZRpKEWFNUCgc4YhOC5yMEK1JO69VQ>?	NVW2VnFoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NFU6PDZpPkK0PFA2QTR4PD;hQi=>
MCF7	NHfTWI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3;BflAvODFvMUCwJO69VQ>?	MlXDOFghcA>?	NU\ncmtSTE2VTx?=	M{fmUGlEPTB;MD63N{DPxE1?	NG[xTGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3NVkxPCd-MkO4O\|E6ODR:L3G+
HCT116	NFq0ZYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MoPjNE4xOS1zMECg{txO	NELpeVA1QCCq	MYHEUXNQ	NVXrV\|BrUUN3ME2xMlE3KM7:TR?=	Mly1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{G5NFQoRjJ\|OEexPVA1RC:jPh?=
U87MG	M17hTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXiwMlAyNTFyMDFOwG0>	MYi0PEBp	NV3zVpZ5TE2VTx?=	Mon2TWM2OD1zLkOyJO69VQ>?	M{fOWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEexPVA1Lz5{M{i3NVkxPDxxYU6=
HBC4	NF;ucnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUDIO49kOC5yMT2xNFAh\|ryP	M{DIU\|Q5KGh?		MlK2UI9oKEeLNUC9MVgvPjFiTR?=	NYTGU4ZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
BSY1	M3;nXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MnTUNE4xOS1zMECg{txO	NInPcYw1QCCq		NHjS[4ZNd2diR1m1NF0uQC52ODDN	MkXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
HBC5	Mke2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NGrQ[5gxNjBzLUGwNEDPxE1?	Mo[0OFghcA>?		MontUI9oKEeLNUC9MVcvQDdiTR?=	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
MCF7	NYnre\|JQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NX;X[IZmOC5yMT2xNFAh\|ryP	NVPBNoE1PDhiaB?=		NF7QcJNNd2diR1m1NF0uQC5zNjDN	NF7tVnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
MDA-MB-231	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4flSVAvODFvMUCwJO69VQ>?	MVq0PEBp		M3[1WWxw\yCJSUWwQU05NjB7IF2=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
U251	NX\PSXBST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NULHfm1tOC5yMT2xNFAh\|ryP	MYO0PEBp		NGO3dFdNd2diR1m1NF0uPy54NjDN	NFHDUmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
SF268	NFXtO\|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3vBbVAvODFvMUCwJO69VQ>?	NWDHbnptPDhiaB?=		NHjENHdNd2diR1m1NF0uPy57MjDN	NXHSS5p{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
SF295	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M{nWUVAvODFvMUCwJO69VQ>?	M{LkXlQ5KGh?		NFLFfnhNd2diR1m1NF0uQC5\|MTDN	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
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KM12	NI\4XIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkXnNE4xOS1zMECg{txO	MVW0PEBp		MkTEUI9oKEeLNUC9MVcvPTRiTR?=	NYH1fpRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
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NCI-H226	M1K5[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{[5ZVAvODFvMUCwJO69VQ>?	MUO0PEBp		MWfMc4chT0l3ME2tPE41PCCP	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
NCI-H522	M2jQU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYCwMlAyNTFyMDFOwG0>	NVvZUHREPDhiaB?=		MkjpUI9oKEeLNUC9MVgvPDViTR?=	M3qyTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
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OVCAR3	M{XGbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NIXHdWYxNjBzLUGwNEDPxE1?	M4fzNFQ5KGh?		Mm[yUI9oKEeLNUC9MVgvOTdiTR?=	M161dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
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OVCAR5	NWH3clRPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXPx[optOC5yMT2xNFAh\|ryP	MYm0PEBp		NX;qRW5nVG:pIFfJOVA:NTdwNUegUS=>	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
OVCAR8	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NV6y[IlUOC5yMT2xNFAh\|ryP	M2HKcVQ5KGh?		MoH5UI9oKEeLNUC9MVcvQDRiTR?=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
SKOV3	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVuwMlAyNTFyMDFOwG0>	NIPJWXQ1QCCq		M3PFR2xw\yCJSUWwQU05NjRzIF2=	NXPrUZlTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzN\|YzPDZpPkKyN\|M3OjR4PD;hQi=>
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ACHN	MnTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHHUemUxNjBzLUGwNEDPxE1?	MorvOFghcA>?		Mlz4UI9oKEeLNUC9MVcvQTViTR?=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
St-4	MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1XwWFAvODFvMUCwJO69VQ>?	M360bFQ5KGh?		MVPMc4chT0l3ME2tO{41PyCP	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN\|NkK0Okc,OjJ\|M{[yOFY9N2F-
MKN1	MlfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFO3bo0xNjBzLUGwNEDPxE1?	NGTSPJY1QCCq		MWfMc4chT0l3ME2tPE41OSCP	MlzYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
MKN7	NEe5S\|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mn[zNE4xOS1zMECg{txO	M1v5R\|Q5KGh?		MmrQUI9oKEeLNUC9MVcvPjhiTR?=	Ml\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
MKN28	NGm2OZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVqwMlAyNTFyMDFOwG0>	NXPXVpMxPDhiaB?=		NHjRb41Nd2diR1m1NF0uPy5yNzDN	NFvQSGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOzOlI1Pid-MkKzN\|YzPDZ:L3G+
MKN45	MkTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MUewMlAyNTFyMDFOwG0>	NEjDPY01QCCq		MUXMc4chT0l3ME2tPE4{PCCP	M1ziWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{O2NlQ3Lz5{MkOzOlI1PjxxYU6=
MKN74	Ml70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MViwMlAyNTFyMDFOwG0>	Mk\GOFghcA>?		NX\Z[WdEVG:pIFfJOVA:NTdwNEKgUS=>	Ml;TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|M{[yOFYoRjJ{M{O2NlQ3RC:jPh?=
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Trypanosoma brucei brucei	NXfOUnJPTnWwY4Tpc44hSXO\|YYm=	MnPCNlAxKG6P	MXyxNkBp	NWnPe2xWTE2VTx?=	NYTmRZR5TGWlcnXhd4UhcW5icHjvd5Bpd3SrZInsJIlvd3OrdH;sJJBpd3OyaHH0[UBt\X[nbB?=	NWC2bmJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NFU6PDZpPkK0PFA2QTR4PD;hQi=>
Trypanosoma brucei brucei	Mln3SpVv[3Srb36gRZN{[Xl?		MoHkNVghcA>?	MUPEUXNQ	MUTJcohq[mm2aX;uJI9nKHKnY3XweI9zNW2nZHnheIVlKGWwZH;jfZRwe2m\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHS{YX7z[oVzemmwIIXweIFs\Q>?	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhyNUm0Okc,OjR6MEW5OFY9N2F-
Trypanosoma brucei brucei	NI[0VVZHfW6ldHnvckBCe3OjeR?=	MXOyNFAhdk1?	NUS5UmRtOTJiaB?=	NGTsd3JFVVOR	NU\zSGtvTW[oZXP0JI9vKHOyaHnu[49ugWynaX6gcIV3\Wx?	NE\nfJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEiwOVk1Pid-MkS4NFU6PDZ:L3G+
Trypanosoma brucei brucei	NW\BXGV7TnWwY4Tpc44hSXO\|YYm=	NHXjd5IzODBibl2=	NHrKNlcyOiCq	NVrT[5NTTE2VTx?=	NGPRVVBG\m[nY4Sgc44heGixc4DoZZRq\HmuY3jvcIlv\SCuZY\lcC=>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhyNUm0Okc,OjR6MEW5OFY9N2F-
UM-UC-3	NX:4fZlqT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MUfJR\|UxRTNzNTDwUS=>	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
SBC-1	NI\QToZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWnjOZVtUUN3ME23OFIheE1?	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MKN1	MnnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NVrRXoY6UUN3ME2yMlghdk1?	M4e5NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NCI-H720	NXraSWJwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGXRNG9KSzVyPUOuOVchdk1?	MnzyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
BFTC-909	MkPzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NVPGb4NnUUN3ME2zMlcyKG6P	M4rVcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
BPH-1	M4XsXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M17p[mlEPTB;ND63OkBvVQ>?	MojPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
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AN3-CA	M4jQ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4nocWlEPTB;NT61OkBvVQ>?	M4nJSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
HN	NVWxb5JUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFjZWlZKSzVyPUWuPVYhdk1?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
HCC2218	M4fnNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnXITWM2OD14Lk[xJI5O	M{TwNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
KP-4	MkHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYXJR\|UxRTdibl2=	NGTjO3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
LU-139	M2XMVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3e0[2lEPTB;Nz63O{BvVQ>?	NVfUS5c3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
ETK-1	M3eyXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoPoTWM2OD15Lki4JI5O	NHLTcI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10	NVi3Z2dyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnfJTWM2OD16LkS5JI5O	M3XN[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
MHH-PREB-1	NYPlTHh6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NV;QOZpqUUN3ME25MlEzKG6P	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
DU-4475	NXTteY5qT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVrPRW9xUUN3ME25MlQ1KG6P	NVTkTnkyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H1666	MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3\zWWlEPTB;OT63NUBvVQ>?	M2nP[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
CAMA-1	M4jQNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NIrjNnFKSzVyPUmuPEBvVQ>?	MmrtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
TYK-nu	NHTTbW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUm3WI1jUUN3ME25Mlk1KG6P	NX\CR2FLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
MFE-280	M{PjN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{\yVmlEPTB;OT65PUBvVQ>?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
A549	NWTBNGxDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXLBPWhsUUN3ME2xNE4yPCCwTR?=	NXPw[lc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
769-P	NVjZcmtIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NV\jWIwxUUN3ME2xNE41PCCwTR?=	NE\uXo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
L-363	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MlPMTWM2OD1zMD62PUBvVQ>?	NF7UXZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
ES8	MonmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFj4Oo5KSzVyPUGwMlkhdk1?	NVfxWmw3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
PA-1	NGPnSWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWrJR\|UxRTFzLkiyJI5O	NI\vTVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
Mewo	NEHiVGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYeySpRvUUN3ME2xNU46QSCwTR?=	NHrGWY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
HSC-3	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHnBVYNKSzVyPUGyMlIyKG6P	NGnUdnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
H4	NGjWNmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYrWUpZjUUN3ME2xNk43PyCwTR?=	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
BB65-RCC	M4jLdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmnuTWM2OD1zMj63NkBvVQ>?	NFfpeVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
NCI-H1793	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MVPJR\|UxRTF{LkiyJI5O	M1i4c\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
KARPAS-45	NX;pZpp1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFm2cGtKSzVyPUGzMlUyKG6P	MkLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
GCT	NGKySYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWPROJI6UUN3ME2xN{43QCCwTR?=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
EoL-1	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFu2[nZKSzVyPUGzMlk{KG6P	M2n1NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
LB2241-RCC	M334b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Mk\qTWM2OD1zND6wNUBvVQ>?	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
ES5	M3GwOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVe2d4dYUUN3ME2xOE44PCCwTR?=	MmjBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LOXIMVI	M4rEfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWfNSYNGUUN3ME2xOE46QSCwTR?=	NHPIfos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
COR-L105	MljYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NGWxcoZKSzVyPUG1MlA1KG6P	MmLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
P12-ICHIKAWA	MlvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MoPqTWM2OD1zNT6xJI5O	NGXVW\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
CHL-1	NH7XSWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlPrTWM2OD1zNT60OUBvVQ>?	NG\XeHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
VMRC-RCZ	NYS4[XNvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnezTWM2OD1zNT62N{BvVQ>?	MmXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
HH	M363bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M13MUWlEPTB;MUWuO{BvVQ>?	MnHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
NUGC-3	M{XQOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXrJR\|UxRTF4LkO0JI5O	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
EGI-1	MlPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUnJR\|UxRTF4LkSzJI5O	M3nPOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
OS-RC-2	NXfqb\|dMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MoTWTWM2OD1zNj60O{BvVQ>?	MkTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
MOLT-16	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3rzeGlEPTB;MUeuNVUhdk1?	NH3Xc3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
NCI-H209	M3\Te2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEO4e2NKSzVyPUG3MlI6KG6P	M3HzdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
697	M13UXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NX\T[4tSUUN3ME2xO{41OyCwTR?=	NEDuemg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
D-56MG	MlP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXfJR\|UxRTF5Lk[5JI5O	M1u3dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
VA-ES-BJ	M161cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmXSTWM2OD1zNz64N{BvVQ>?	NF;0[pg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
EW-16	M3K4fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NE\QSXhKSzVyPUG4MlA1KG6P	Mn;oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
HGC-27	M{m1cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4LLc2lEPTB;MUiuNFYhdk1?	NE\YU\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
ES7	NUHke2N7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnK3TWM2OD1zOD6wPUBvVQ>?	M3zrUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
HuH-7	NITDWXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1q3bmlEPTB;MUiuOEBvVQ>?	M4nzV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
BCPAP	NXrrOJFWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEnpR49KSzVyPUG4MlY2KG6P	Ml\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
SW1710	MonMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlnpTWM2OD1zOD63NkBvVQ>?	NH24UWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
IST-MEL1	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFnoenZKSzVyPUG4Mlc4KG6P	NEjjTVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
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SW1088	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmfRTWM2OD1{OD6yPUBvVQ>?	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
A204	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NX3LRo9{UUN3ME2yPE41PyCwTR?=	MoPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
A3-KAW	NVHjWVBMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn;kTWM2OD1{OD62OEBvVQ>?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MV-4-11	M2HCUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWPJfoRuUUN3ME2yPE44KG6P	NVjwNIZiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
ES1	NXPs[GIyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlPzTWM2OD1{OD64OEBvVQ>?	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
ONS-76	NXrQTVNRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlX6TWM2OD1{OT6wOUBvVQ>?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
D-263MG	NV7xdphOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NUixeFlHUUN3ME2yPU4{PSCwTR?=	NYDXOGIzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
U-2-OS	MoHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXfJR\|UxRTJ7LkWgcm0>	MmfRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
T98G	M2r2TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2PxXGlEPTB;MkmuPFghdk1?	NX7JV3BURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
GI-ME-N	Ml76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYHJR\|UxRTJ7LkmxJI5O	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
BHT-101	MlvHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MkK0TWM2OD1\|MD6wOUBvVQ>?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
NOS-1	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXXJR\|UxRTNyLkGyJI5O	NGHHWlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
AsPC-1	MlTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2\iZWlEPTB;M{CuNkBvVQ>?	NHyyXmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
QIMR-WIL	M1v0dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnizTWM2OD1\|MD62JI5O	NF75T489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
JVM-3	NXizVoJpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWLSSYs{UUN3ME2zNE45OyCwTR?=	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
KU-19-19	M4nkd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NIL1fFFKSzVyPUOxMlE{KG6P	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
OVCAR-8	NUfn[YtTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MofGTWM2OD1\|MT6yOkBvVQ>?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
BEN	NHrRN49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MofQTWM2OD1\|MT62N{BvVQ>?	MofMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
BE-13	Ml;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYLJR\|UxRTNzLkiyJI5O	M1XNVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
EW-18	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkjoTWM2OD1\|MT64OkBvVQ>?	NFT3SVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
ECC10	NU\KXnhnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NH7wdXJKSzVyPUOxMlk2KG6P	M1;EVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
CTV-1	M1WzSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVr0W\|FrUUN3ME2zNk45OiCwTR?=	NVrveWd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
COLO-800	NYjIPVRFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NYjSNYxoUUN3ME2zNk46OiCwTR?=	NVvPbI5vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
A2058	NEfze29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWXJR\|UxRTN\|LkK1JI5O	NGLjU5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
VM-CUB-1	NHO1[ZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MonLTWM2OD1\|Mz60N{BvVQ>?	NVfmXFZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
Ca9-22	NV7nSmhRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWnoV45uUUN3ME2zN{42PSCwTR?=	M3PafFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
COLO-684	M1nHTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGXwXFFKSzVyPUOzMlU5KG6P	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
RPMI-8226	M1\JOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{\idmlEPTB;M{OuPFIhdk1?	MmXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
SK-NEP-1	MkDnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2K4fGlEPTB;M{SuNVUhdk1?	M3PPU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
Becker	NFX5dnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NGXwPJpKSzVyPUO0MlIyKG6P	M{HXcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NCI-H28	MkTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVTJR\|UxRTN2LkS0JI5O	M{jPVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
CAL-120	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVXZR4Y5UUN3ME2zOE44OiCwTR?=	M3XO[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
MDA-MB-157	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUnDPHRHUUN3ME2zOU41OSCwTR?=	NGLWbGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
OVCAR-5	NFjEXWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4nmXWlEPTB;M{WuOFchdk1?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
LAMA-84	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHvSSXVKSzVyPUO1Mlg4KG6P	NY[xUpVZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
CAKI-1	M{C2ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFzMTY1KSzVyPUO2MlQ{KG6P	MlXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
Daoy	NEnh[ZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{HYR2lEPTB;M{[uO{BvVQ>?	NX;aT2o1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
CAL-62	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4HqUWlEPTB;M{[uPFYhdk1?	MmK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
8-MG-BA	Ml2xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXHoelZZUUN3ME2zO{4zPSCwTR?=	NWjUOGMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
8505C	NW\EcVdET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHPGfWJKSzVyPUO3MlUyKG6P	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
LNCaP-Clone-FGC	M{jJPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MUPJR\|UxRTN5LkW5JI5O	NX3WbphERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
CAL-39	M{Hnemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV76UoJEUUN3ME2zO{46KG6P	M4O4eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
MN-60	NWPzPJBJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2D1[mlEPTB;M{euPVYhdk1?	NXfCZ4NLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
RKO	NXSzZ3diT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3nqXWlEPTB;M{iuN\|Mhdk1?	MnLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
U251	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkToTWM2OD1\|OD60N{BvVQ>?	M3qzV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SJSA-1	Mkj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MX\JR\|UxRTN6LkW0JI5O	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
CAL-12T	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MVXJR\|UxRTN6Lk[gcm0>	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
HCC1419	NXX4U4dsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2naRWlEPTB;M{iuO\|Ehdk1?	NFHtcGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
CO-314	MljwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnXlTWM2OD1\|OD63OEBvVQ>?	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
LB1047-RCC	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MULJR\|UxRTN6LkixJI5O	MnHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
A101D	M{DDfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUCwe5FFUUN3ME2zPE46PyCwTR?=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MDA-MB-175-VII	NVm5dIM4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2H5NGlEPTB;M{muOVEhdk1?	M4PXS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
MZ2-MEL	MoDPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1f1fGlEPTB;M{muOlYhdk1?	M1nsN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NB5	NGfS[oRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NXuzO3FUUUN3ME20NE43PCCwTR?=	NWD3fY1tRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
G-401	NHG3UWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2rBZ2lEPTB;NECuPFIhdk1?	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
HT	NGHJPFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnXnTWM2OD12MT6wNkBvVQ>?	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
8305C	M2rvVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEHpUZpKSzVyPUSxMlg4KG6P	MoDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
BHY	NV3zS3B1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn\jTWM2OD12Mj63NkBvVQ>?	M2izVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NTERA-S-cl-D1	MlTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NEjRVpdKSzVyPUSzJI5O	NUfWOYFzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
T-24	Ml7mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYHJR\|UxRTR\|LkizJI5O	NI\WU5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
EPLC-272H	NXzzcm82T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHrrUlNKSzVyPUS0MlM6KG6P	M1:xS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
PSN1	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWDJR\|UxRTR2LkSgcm0>	Mmr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
NCI-H2452	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkLqTWM2OD12ND61PEBvVQ>?	NWCxRlVmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
KS-1	MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NYHGb2hvUUN3ME20OE43PiCwTR?=	NVHXfm9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
HD-MY-Z	NVrteZRuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3ruWWlEPTB;NESuPVIhdk1?	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
KNS-62	M2S5WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3vCb2lEPTB;NEWuN\|ghdk1?	NH7uS5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
NOMO-1	NF7SV5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2POW2lEPTB;NEWuPFchdk1?	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
SK-HEP-1	M{TWVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWOyN4dKUUN3ME20Ok4xPCCwTR?=	NVzPTIlWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
IGR-1	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NX[wU3h5UUN3ME20Ok4yOyCwTR?=	NIr3SG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
KYSE-180	MnPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NX3McJF1UUN3ME20Ok4yPyCwTR?=	NF\HSpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
EW-22	NIf0WmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4LBeWlEPTB;NE[uNkBvVQ>?	NVTCPXMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H1355	NGnMepVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX7JR\|UxRTR4LkO0JI5O	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
DOHH-2	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXXJR\|UxRTR4LkO3JI5O	NFjiNmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
A4-Fuk	M325S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MV7JR\|UxRTR4LkWyJI5O	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
PC-14	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWfzeIF6UUN3ME20Ok42PyCwTR?=	MmrpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
TE-5	NFu1[3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVTJR\|UxRTR4Lki0JI5O	MoDGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LB996-RCC	NWrMfGNJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NF6yNI9KSzVyPUS3MlE6KG6P	NV23XoZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H1623	MnPKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmC2TWM2OD12Nz63OkBvVQ>?	M1zjd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NCI-H1155	NWLWZ25pT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NYfy[Vd6UUN3ME20PE4{OiCwTR?=	Ml62QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LoVo	M1m5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NIjNPHRKSzVyPUS4MlQhdk1?	MlvmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
MCF7	M1fP[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnLLTWM2OD12OD61PEBvVQ>?	M1fr[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
AGS	NFj6d41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUnFTFJYUUN3ME20PE44PyCwTR?=	NYTTUm53RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
PFSK-1	NX7OSHRbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NH;ZR3FKSzVyPUS5MlA3KG6P	NULieJZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
22RV1	M33nXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NYToN2RrUUN3ME20PU4xQSCwTR?=	NFG3Ro89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
COR-L23	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYnJR\|UxRTR7LkG2JI5O	NYnnUWlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
D-283MED	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4TpUGlEPTB;NEmuOlEhdk1?	MkTwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
SW982	NYmw[VFtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGT3e4dKSzVyPUS5MlkyKG6P	NIrNfFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
SW620	MnXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NVqybVlwUUN3ME21NE4{PiCwTR?=	NEPiZ489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
KYSE-140	NGXLOWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NEPUT3hKSzVyPUWwMlU2KG6P	NVHvUolqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
GCIY	MnHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFWzOWdKSzVyPUWwMlY4KG6P	NEjMTlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
MHH-ESS-1	NYLsSJZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MVjJR\|UxRTVzLkm1JI5O	MnHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
D-423MG	Mom2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1ixZWlEPTB;NUKuNFghdk1?	NXX4fYxJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
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RPMI-2650	NYHzZlR3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWnCWmlYUUN3ME21Nk43QSCwTR?=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
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NCI-H1304	NWjSNVlnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVrC[mtIUUN3ME2xMlY2PDl6IN88US=>	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
KARPAS-299	M33je2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmnRTWM2OD1zLke5OFc6KM7:TR?=	MnrHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
NCI-H358	NFz1UJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUXF[VBUUUN3ME2xMlk2PTB\|IN88US=>	NVTCfmFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
OAW-28	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Ml3HTWM2OD1{LkC2PVE6KM7:TR?=	NEflcXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
C8166	MlS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4rYSmlEPTB;Mj62N\|M6OiEQvF2=	MkjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
COLO-678	M{nPb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEfQeIlKSzVyPUKuPFM4PjhizszN	NWPrXZpiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
SK-N-DZ	NGCzVVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVXqZYxLUUN3ME2yMlg3OzhzIN88US=>	NW[wdJJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
NCI-H82	NX\FNFFlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnrDTWM2OD1{LkmwN\|Y6KM7:TR?=	NUHOUoQ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
JVM-2	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NIHINJpKSzVyPUOuNFMyQDhizszN	MmO4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
SW1116	NWi4OmFwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1PyPGlEPTB;Mz6wOVQyOSEQvF2=	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
MDA-MB-453	NF3wS4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX\JR\|UxRTNwMUC0N\|kh\|ryP	MnHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
Calu-6	NWLPN\|NyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MojITWM2OD1\|LkG5PVch\|ryP	M136dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
SHP-77	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnLHTWM2OD1\|LkWyPEDPxE1?	NXvtNGY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
Capan-2	M1nrVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWnJR\|UxRTRwM{K1PFEh\|ryP	NXn1N\|ZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
SW948	NG\Qc3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUHJR\|UxRTRwN{S3O\|kh\|ryP	NWXN[pp4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
HDLM-2	NUHOfHB3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkfZTWM2OD13LkC3O\|I2KM7:TR?=	MkPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LAN-6	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MoSyTWM2OD14LkKxOFk2KM7:TR?=	MlrQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
LU-135	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWTE[VM6UUN3ME22MlQ{ODd5IN88US=>	NYXDNnI4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
T84	NIPaV\|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MoXmTWM2OD14Lkm4OlQh\|ryP	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
CAPAN-1	MlPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MX3JR\|UxRTdwNkKyN\|Mh\|ryP	NFK2R3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
SW1417	NIDYfIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2HsemlEPTB;Nz65NVc{OSEQvF2=	NFXh[2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDd7NE[zM{c,W0GQR1XSQE9iRg>?
BALL-1	M3v5Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYfJR\|UxRThwNUC0NFkh\|ryP	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
HT-29	NVyyNGZzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MmrETWM2OD17LkC3OVk6KM7:TR?=	M4LSblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
C3A	M{\3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVLpe\|NxUUN3ME25Mlg5Ozl2IN88US=>	M3TMTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
NCI-H1993	NH2wT\|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFuwfVhKSzVyPUGwMlUxQDFizszN	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
SK-MEL-1	NIX2Oo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M3PaR2lEPTB;MUGuOlI5QSEQvF2=	NYnLVo14RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh5OUS2N{8oRlODTlfFVlww[T5?
DU-145	NGLkWmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX7JR\|UxRTF2LkWwOVIh\|ryP	MlPpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
KP-N-YN	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYrJR\|UxRTF2LkWxNFMh\|ryP	Mn30QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
CCRF-CEM	NH7TU\|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUTPPFI6UUN3ME2xOE44QDB7IN88US=>	M4HUelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5Pzl2NkOvK\|5USU6JRWK8M4E,
ZR-75-30	MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3nDZ2lEPTB;MU[uN\|MxOyEQvF2=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>
A673	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NIrre4hKSzVyPUKwMlU2ODdizszN	Ml7wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg4QTR4Mz:nQnNCVkeHUkyvZV4>
K-562	M{i2OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFzTcmdKSzVyPUK4MlQyQDNizszN	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFc6PDZ\|Lze+V2FPT0WUPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Myc / Vinculin / pT58 Myc; PubMed: 25934076 Cancer Discov Immunoblots documenting MYC and pT58 MYC stability. SW480 cells were treated with 200nM BEZ235 or solvent control for 24h and cycloheximide (50µg/ml) and harvested at the indicated time points. Vinculin was used as loading control. Exposures of MYC and pT58 MYC blots were adjusted to equalize exposure at 0 min (n=3; unless otherwise indicated, n indicates the number of independent biological repeat experiments in the following legends). pAkt / Akt / Skp2 / AR / pS6 / p27; PubMed: 24347472 Prostate BEZ235 decreases Skp2 and increases AR protein expression inC4-2B cells. Cells were treated with BEZ235 at various doses. Cell lysates were subjected to Westernblot analysis with indicated antibodies. p-S6K / S6K / p-Akt / Akt / p-ABL / BCR-ABL; PubMed: 28435223 Drug Des Devel Ther Dose-dependent inhibition effect of BEZ235 on the phosphorylation levels of S6K, Akt (Ser473), and BCR–ABL in CML cells. Results of Western blot. Bcl-2 / Caspase-3 / Cleaved caspase-3 ; PubMed: 28435223 Drug Des Devel Ther K562 (A1) and KBM7R (A2) cells were treated with BEZ235 for 24 h; total protein lysates were immunoblotted with antibodies for Bcl-2, caspase-3, and cleaved caspase-3. Cyclin D1 / Cyclin D2 ; PubMed: 28435223 Drug Des Devel Ther Dose-dependent inhibition effect of BEZ235 on the expression levels of cyclin D1 and cyclin D2 in K562 and KBM7R cells. Western blot results of K562 (A1) and KBM7R (A2) cells. mTOR / p-mTOR / p70S6K / p-p70S6K ; PubMed: 29581778 J Cancer Eca-109 cells was treated with increasing concentration of BEZ235 (0, 100, 200 and 500 nM) for 48 hours. Western blot showed the inhibition effect of PI3K/mTOR pathway was dose dependent. p-EGFR-Tyr1068 / EGFR / p-HER2-Tyr1221 / p-HER2-Tyr1222 / HER2 / p-HER3-Tyr1289; PubMed: 21278786 Oncogene Immunoblots of protein lysates from BT474 and MCF7-HER2 cells treated with either BEZ235 alone (0–500 n) or the combination of BEZ235 (0–500 n) and the tyrosine kinase inhibitor lapatinib (LAP, 500 n) for 24 h.	25934076 24347472 28435223 29581778 21278786
Immunofluorescence	SP1; PubMed: 26336820 Oncotarget Immunofluorescence assessment of SP1 after BEZ235 treatment for 24 hrs in A673, SKNMC cells and in HFF. p-ErbB2(Y1248); PubMed: 24675532 Cell Res Immunofluorescence staining of BT474.LapR cells grown in 3-D culture under different treatment conditions for a week. Scale bar indicates 50 μm. LC3; PubMed: 23603977 Acta Pharmacol Sin (A) SU-2 cells were treated with NVP-BEZ235 (10 nmol/L) for 12 h and then harvested 24 h after treatment with IR (8 Gy), and analyzed for LC3 expression (top) or the formation of autophagolysosomes by using MDC (middle) and LTR (bottom) by immunofluorescence analysis (600×magnification).	26336820 24675532 23603977
Growth inhibition assay	Cell viability (P3, U87); PubMed: 27542970 BMC Cancer IC50 doses of dactolisib for P3 (left) and U87 (right) glioma cells, generated from MTS assay. Cell viability (breast cancer cells); PubMed: 28060760 Oncotarget Cell viability of breast cancer cells treated by either BEZ235 or TSA for 48h was measured by MTT assay. Each cell line was analyzed in triplicate, and a representative experiment is shown. Cell viability (K562, KBM7R); PubMed: 28435223 Drug Des Devel Ther (A) K562 cells were treated with increasing concentrations of BEZ235 for 24, 48, or 72 h and subjected to MTS assay. (B) KBM7R cells were treated with increasing concentrations of BEZ235 for 24, 48, or 72 h and subjected to MTS assay. (C) K562 cells were treated with imatinib (0–10 μM) alone or imatinib and BEZ235 (0.2 μM) for 48 h and subjected to MTS assay. (D) KBM7R cells were treated with imatinib (0–10 μM) alone or imatinib and BEZ235 (0.2 μM) for 48 h and subjected to MTS assay. Mean ± SD. n=3. *P<0.05, compared to the control group. #P<0.05, compared to the group of IM alone.	27542970 28060760 28435223
ELISA	VEGF; PubMed: 19671762 Mol Cancer Ther NVP-BEZ235 attenuated VEGF secretion by at least 40% in U87 cells. The VEGF level in the serum-free medium was measured in triplicate and the experiment repeated at least twice to confirm results.	19671762

In vivo

BEZ235 induces regression of the tumors (69%) without statistically significant effect on body weight gain. Altogether, these preliminary in vivo efficacy results show that BEZ235 causes disease stasis when administered orally as a single agent and can enhance the efficacy of other anticancer agents when used in combination studies. ^[1]

Protocol

Kinase Assay: ^[1]	- Collapse In vitro Protein Kinase, PI3K, and mTOR Assays: PI3Kα, β, and δ proteins are composed of the iSH2 domain of p85 NH₂-terminally fused to the full-length protein p110 protein, with the exception of α that also does not contain the last 20 amino acids. PI3Kγ is produced as full-length protein deleted for its first 144 amino acids. All constructs are fused to a COOH-terminal His tag for convenient purification and then cloned into the pBlue-Bac4.5 (for α, β, and δ isoforms) or pVL1393 (for γ isoform) plasmids. The different vectors are then cotransfected with BaculoGold WT genomic DNA using methods recommended by the vendor for production of the respective recombinant baculoviruses and proteins. BEZ235 are tested for their activity against PI3K using a Kinase-Glo assay. The kinase reaction is done in 384-well black plate. Each well is loaded with 50 μL of test items (in 90% DMSO) and 5 μL reaction buffer containing 10 μg/mL PI substrate (l-α-phosphatidylinositol; Avanti Polar Lipids; prepared in 3% octyl-glucoside) and the PI3K proteins (10, 25, 10, and 150 nM of p110α, p110β, p110δ, and p110γ, respectively) are then added to it. The reaction is started by the addition of 5 μL of 1 μM ATP prepared in the reaction buffer and is incubated for either 60 (for p110α, p110β, and p110δ) or 120 min (for p110γ). It is terminated by the addition of 10 μL Kinase-Glo buffer. The plates are then read in a Synergy 2 reader for luminescence detection.
Cell Research: ^[2]	- Collapse Cell lines: HCT116, DLD-1 and SW480 cells Concentrations: 0-1 μM Incubation Time: 48 hours Method: The human CRC cell lines, HCT116 (PIK3CA mutant; kinase domain at H1047R), DLD-1 (PIK3CA mutant; helical domain at E545K), and SW480 (PIK3CA wild-type) and isogenic DLD-1 PIK3CA mutant as well as wild-type cells are maintained in DMEM with 10% FBS and 1 × Penicillin/Streptomycin. Cells are plated at different initial densities (HCT116: 3 × 10³ cells/well, DLD-1: 5.5 × 10³ cells/well, SW480: 4.5 × 10³ cells/well, DLD-1 PIK3CA mutant: 7 × 10³ cells/well, and DLD-1 PIK3CA wild-type: 9 × 10³ cells/well) to account for differential growth kinetics. After 16 hours, cells are incubated with increasing concentrations of BEZ235, and the drug-containing growth medium is changed every 24 hours. Cell viability is assessed 16 hours after the initial plating and 48 hours after initiation of drug treatment using the colorimetric MTS assay CellTiter 96® AQueous One Solution Cell Proliferation Assay, as per the manufacturer's instructions. Cell viability after drug treatment is normalized to that of untreated cells also grown for 48 hours. For western blot analysis, cells are plated with zero or maximum inhibitory dose (500 nM) BEZ235 for 2, 6, 24, or 48 hours. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Female Harlan athymic nude mice Dosages: 45 mg/kg Administration: p.o. (Only for Reference)

References

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Solubility (25°C)

In vitro	DMF	18 mg/mL warmed (38.33 mM)
	DMSO	0.01 mg/mL (0.02 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): NMP+PEG300 (10+90, v+v) For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Dactolisib (BEZ235) SDF

Molecular Weight	469.55
Formula	C₃₀H₂₃N₅O
CAS No.	915019-65-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NVP-BEZ235
Smiles	CC(C)(C#N)C1=CC=C(C=C1)N2C3=C4C=C(C=CC4=NC=C3N(C2=O)C)C5=CC6=CC=CC=C6N=C5

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Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01856101	Terminated	Drug: BEZ235	Carcinoma Transitional Cell	Cliniques universitaires Saint-Luc- Université Catholique de Louvain\|Novartis	February 2013	Phase 2
NCT01717898	Terminated	Drug: BEZ235\|Drug: Prednisone\|Drug: Abiraterone acetate	Castrate-resistant Prostate Cancer	Charles Ryan\|Novartis Pharmaceuticals\|University of California San Francisco	January 31 2013	Phase 1\|Phase 2
NCT01756118	Unknown status	Drug: BEZ235	Acute Lymphoblastic Leukemia\|Leukemia Myelocytic Acute\|Chronic Myelogenous Leukemia With Crisis of Blast Cells	Johann Wolfgang Goethe University Hospital	June 2012	Phase 1
NCT01290406	Withdrawn	Drug: BEZ235	Endometrial Cancer	Novartis Pharmaceuticals\|Novartis	March 2012	Phase 2
NCT01288092	Withdrawn	Drug: BEZ235	Metastatic Breast Cancer	Novartis Pharmaceuticals\|Novartis	March 2012	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
With difficulties in dissolving BEZ235, How can I improve its solubility?
Answer:
For in vivo study, we suggest the vehicle solution NMP/polyethylene glycol 300 (10/90, v/v). Please dissolve BEZ235 in NMP as more as possible. You can sonicate and warm it in water bath for a while. Then dilute with PEG 300.

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

Selective PI3K Inhibitors

CAL-101 (Idelalisib, GS-1101) : p110δ-selective, IC50=2.5 nM.
Selective PI3K Inhibitors

TGX-221 : p110β-specific, IC50=5 nM.
Most Potent PI3K Inhibitor

Duvelisib (IPI-145, INK1197) : PI3K δ, IC50=1 nM.
FDA-approved PI3K Inhibitor

CAL-101 (Idelalisib, GS-1101) : Approved by FDA for Relapsed Chronic Lymphocytic Leukemia, Follicular Lymphoma and Small Lymphocytic Lymphoma.
Newest PI3K Inhibitor

GSK2636771 : Potent, orally bioavailable, PI3Kβ-selective inhibitor, sensitive to PTEN null cell lines.

Related PI3K Products

Taselisib (GDC 0032) ^New

Taselisib (GDC 0032, RG7604) is a potent, next-generation β isoform-sparing PI3K inhibitor targeting PI3Kα/δ/γ with K_i of 0.29 nM/0.12 nM/0.97nM, >10 fold selective over PI3Kβ.

Features:A beta isoform-sparing PI3K inhibitor.
Pilaralisib (XL147) ^New

Pilaralisib (XL147) is a selective and reversible class I PI3K inhibitor for PI3Kα/δ/γ with IC50 of 39 nM/36 nM/23 nM in cell-free assays, less potent to PI3Kβ. Phase 1/2.
LY294002

LY294002 (SF 1101, NSC 697286) is the first synthetic molecule known to inhibit PI3Kα/δ/β with IC50 of 0.5 μM/0.57 μM/0.97 μM, respectively; more stable in solution than Wortmannin, and also blocks autophagosome formation. It not only binds to class I PI3Ks and other PI3K-related kinases, but also to novel targets seemingly unrelated to the PI3K family. LY294002 also inhibits CK2 with IC50 of 98 nM. LY294002 is a non-specific DNA-PKcs inhibitor and activates autophagy and apoptosis.
3-Methyladenine (3-MA)

3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.
Idelalisib (CAL-101, GS-1101)

Idelalisib (CAL-101, GS-1101) is a selective p110δ inhibitor with IC50 of 2.5 nM in cell-free assays; shown to have 40- to 300-fold greater selectivity for p110δ than p110α/β/γ, and 400- to 4000-fold more selectivity to p110δ than C2β, hVPS34, DNA-PK and mTOR. Idelalisib also stimulates autophagy.
Wortmannin (KY 12420)

Wortmannin (KY 12420, SL-2052, BRN 0067676, NSC 627609) is the first described PI3K inhibitor with IC50 of 3 nM in a cell-free assay, with little selectivity within the PI3K family. Wortmannin blocks autophagosome formation and potently inhibits DNA-PK/ATM with IC50 of 16 nM and 150 nM in cell-free assays. Wortmannin also inhibits PLK1 activity.
Buparlisib (BKM120)

Buparlisib (BKM120, NVP-BKM120) is a selective PI3K inhibitor of p110α/β/δ/γ with IC50 of 52 nM/166 nM/116 nM/262 nM in cell-free assays, respectively. Reduced potency against VPS34, mTOR, DNAPK, with little activity to PI4Kβ. Buparlisib induces apoptosis. Phase 2.
Pictilisib (GDC-0941)

Pictilisib (GDC-0941, RG7321) is a potent inhibitor of PI3Kα/δ with IC50 of 3 nM in cell-free assays, with modest selectivity against p110β (11-fold) and p110γ (25-fold). Pictilisib (GDC-0941) induces autophagy and apoptosis. Phase 2.
Alpelisib (BYL719)

Alpelisib (BYL719) is a potent and selective PI3Kα inhibitor with IC50 of 5 nM in a cell-free assay, and minimal effect on PI3Kβ/γ/δ. Phase 2.

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Dactolisib (BEZ235)