Z-VAD-FMK

Catalog No.S7023 Synonyms: Z-VAD(OMe)-FMK

For research use only.

Z-VAD-FMK (Z-VAD(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

Z-VAD-FMK Chemical Structure

CAS No. 187389-52-2

Selleck's Z-VAD-FMK has been cited by 793 publications

Purity & Quality Control

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Biological Activity

Description Z-VAD-FMK (Z-VAD(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Features A key compound for apoptosis studies.
Targets
Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro

Z-VAD-FMK (10 μM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 μM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U937 MV\BdI9xfG:|aYOgRZN{[Xl? NVPqPGpRPTEQvF2= M2f3cVQ5cA>? MnT6doVlfWOnczDj[YxtKGSnYYToJINifXOnZDDifUBUU0lvSVmgeJJm[XSvZX70xsA> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NEC0N{c,OjV6MkSwOFM9N2F-
HL-60 NIfrdWtCeG:ydH;zbZMhSXO|YYm= MWW1NO69VQ>? MUm0PIg> MV3y[YR2[2W|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JHNMUS2LSTD0doVifG2nboVCpC=> NEnhRpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOFA1Oyd-MkW4NlQxPDN:L3G+
DTK-SME M2PqSmFxd3C2b4Ppd{BCe3OjeR?= MV21NO69VQ>? MX[ybC=> NH7ZRpVFVVOR M1vGcJBienSrYXzsfUBqdmirYnn0d{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgZpVxcX[jY3HpcoU> NXu1OY9LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OFM5QTdpPkK1PFQ{QDl5PD;hQi=>
HCT116  NEPGOo5CeG:ydH;zbZMhSXO|YYm= MXG1NO69VQ>? Mm[5Nog> NHyyUIlqdmirYnn0d{B1cGViY3XscEBl\WG2aDDpcoR2[2WmIHL5JGRFXlB? Mo\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6Nki4NVgoRjJ3OE[4PFE5RC:jPh?=
SGN NYT6THN6SXCxcITvd4l{KEG|c3H5 NXHI[Ic{OjCvTR?= M2\sOlQ5cA>? MnO2bIF{KG6xIHnu[ox2\W6lZTDvckBCUUZuIHPhcJBicW5iZYjwdoV{e2mxbjDvdkBk\WyuIHHwc5B1d3Orcx?= NWTFU3YxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4O|Q3OzNpPkK1PFc1PjN|PD;hQi=>
A549/V16 MlPuRZBweHSxc3nzJGF{e2G7 NWnPem9KPTEQvF2= Ml3BNYg> M360e5JmeHKnc4Pld{BVTy:WTT3pcoR2[2WmIHHwc5B1d3Orcx?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl2NkCzN{c,OjV7NE[wN|M9N2F-
U1 MnXOT4lv[XOnIFHzd4F6 MV:xNFDPxE1? NXLrPIdjOmh? MX7pcohq[mm2czDjZZNx[XOnLUO= NE\UbmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4NFk1Oid-MkW5PFA6PDJ:L3G+
ACH-2 NU\tc|E4SXCxcITvd4l{KEG|c3H5 NUPzTGZqOC1{MEFOwG0> MljXNog> MXHy[YR2[2W|IHTyeYcucW6mdXPl[EBieG:ydH;zbZMh[W6mIIP1ZpNmeXWnboSgTGlXNTFicnXwcIlk[XSrb36gbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NYnpPYlvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFA6PDJpPkK1PVgxQTR{PD;hQi=>
U1 M3vGd2Fxd3C2b4Ppd{BCe3OjeR?= NI\VV4QxNTFyMN88US=> NXjve5RSOmh? NVi0PVZtemWmdXPld{BlenWpLXnu[JVk\WRiYYDvdJRwe2m|IHHu[EB{fWK|ZYH1[Y51KEiLVj2xJJJmeGyrY3H0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MmLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEC5OFIoRjJ3OUiwPVQzRC:jPh?=
HUVEC-2c M1S0S2Fxd3C2b4Ppd{BCe3OjeR?= MX61NO69VQ>? M3TlXlZp M3LaU4Rm[3KnYYPl[EB1cGVib4itUGRNNWmwZIXj[YQh[XW2b4DoZYd6 MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB{MUeyPUc,OjZyMkG3Nlk9N2F-
SP2/0 MV;BdI9xfG:|aYOgRZN{[Xl? MVqxNFDPxE1? M{D2c|Fp NUDx[YdVTE2VTx?= MU\icI9kc3NidHjlJIFxd3C2b4Ppd{Bw\iCVUEKvNEBk\Wyucx?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB5NEezNkc,OjZyN{S3N|I9N2F-
CEF NFzkblVMcW6jc3WgRZN{[Xl? MnfqNEw{Oyx4NzyxNFDPxE1? NEXLb2syPW2rbh?= NGLEfVFld3ewLYLl[5Vt[XSnczDQVkBmdnq7bXWgZYN1cX[rdImgeI8hPDExvJWgZZQhOTByzszN MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFyMkOzPUc,OjZzMEKzN|k9N2F-
BEL-7402 MmnmRZBweHSxc3nzJGF{e2G7 NVXlUZp1OjEQvF2= M1XKdVFp MYrheJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMg MlnwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkS3PVUoRjJ4MU[0O|k2RC:jPh?=
HepG2 M3zlZmFxd3C2b4Ppd{BCe3OjeR?= NV7hc4l3OjEQvF2= NX7IWnprOWh? NVXwXYcy[XS2ZX71ZZRm\CC2aHWgZZBweHSxdHnjJIlv\HWldHnvckBw\iCLSVmtNVDDqA>? NFHGXFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG2OFc6PSd-Mk[xOlQ4QTV:L3G+
HGL5 NHH2XW9CeG:ydH;zbZMhSXO|YYm= MXK1NO69VQ>? MY[yOIg> MUTpcoRq[2G2ZYOgZUBxem:2ZXP0bZZmKGWoZnXjeEBi\2GrboP0JIV1d3Cxc3nk[S=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF4OUC3OUc,OjZzNkmwO|U9N2F-
GC1a M4LxRmFxd3C2b4Ppd{BCe3OjeR?= M1HVR|Ux|ryP NULhZ3ZOOjSq NHnONWtqdmSrY3H0[ZMh[SCycn;0[YN1cX[nIHXm[oVkfCCjZ3HpcpN1KGW2b4Dvd4ll\Q>? M1H4VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MU[5NFc2Lz5{NkG2PVA4PTxxYU6=
A549 NHrVfG1CeG:ydH;zbZMhSXO|YYm= MUKyNEDPxE1? NXvjZpAyOjSq NES0PJRz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KEiIQ2CgeJJm[XSvZX70 MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd7NEG0PUc,OjV5OUSxOFk9N2F-
HeLa NEDqSoZCeG:ydH;zbZMhSXO|YYm= MUCxNO69VQ>? M4LVfVQ5cA>? NWr1fpQy\GWlbHnu[ZMhfGinIILheIUhd2ZiYYDvdJRwe2m|IHTyZY1ifGmlYXzsfS=> NXHURW5NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O|I2PDVpPkK1O|czPTR3PD;hQi=>
SGC-7901 NGj5VVFCeG:ydH;zbZMhSXO|YYm= NEfzVGIzOMLizszN M4TWRlFp MonJbY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDvfIFtcXCuYYTpci=> M{fLV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{[3NFc3Lz5{NUe2O|A4PjxxYU6=
MCF-7 NVr3VlNySXCxcITvd4l{KEG|c3H5 M1vROVEx|ryP MXOybC=> NUezUpR1cW6qaXLpeJMhfGinIHPlcIwh\GWjdHigbY5lfWOnZDDifUA{Sg>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd{MkGxOEc,OjV5MkKxNVQ9N2F-
A549 M1;vWGFxd3C2b4Ppd{BCe3OjeR?= M{XLZVExKM7:TR?= MmHMNlRp NEPUS2ZFVVOR MoLidJJwdW:2ZYOgTGJENXS{ZXH0[YQhSTV2OTDj[YxtKHO3co\peoFtKCZiYYT0[Y52[XSnczD0bIUh[2ynYY\l[EBRSVKSIHX4dJJme3Orb36= MoLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEO1OlgoRjJ3NkizOVY5RC:jPh?=
U87 MlrzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfKOFc2OM7:TR?= NUPKeWo{OWh? Ml3JdoVkd3[ncoOgZ4VtdCCpcn;3eIgh\nKxbTDUUXohfHKnYYTt[Y51 MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6MU[2PEc,OjV4OEG2Olg9N2F-
Neocortical Neuron NH3YfndP\XW{b4TvfIlkcXS7IFHzd4F6 NFjRdJUyODEQvF2= NVvTN2VXOWh? MkLHZY51[Wexbnn6[ZMhUG:rYX3p[IUhSS2LbnT1Z4VlKE6ndYLveI95cWOrdIm= M{LDb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nke1NFAyLz5{NU[3OVAxOTxxYU6=
Primary human placental cytotrophoblasts MXjBdI9xfG:|aYOgRZN{[Xl? MWqzNO69VQ>? M4LZNFI1cA>? NYfUVGVmTE2VTx?= M1jlVpJmfmW{c3XzJJRp\SCrbnjpZol1cW:wIH;mJFEy|rJvSGPENkBjgSC2cnnjcI9{[W8EoB?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ2MkW5Nkc,OjV4NEK1PVI9N2F-
MM NVHSZXN[SXCxcITvd4l{KEG|c3H5 MYq1NO69VQ>? MYqyNI1qdg>? NG[xOFZx[XK2bImgbY5pcWKrdIOgV2hMNWmwZIXj[YQhKGOnbHyg[IVifGh? NWPxPGUxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1N|AxQThpPkK1OVMxODl6PD;hQi=>
DLD1 NEXDUpNCeG:ydH;zbZMhSXO|YYm= NHrzdFkzOMLizszN NWqwNWdlOWh? MUjwZZJ1dHlicnX2[ZJ{\XNiY3XscEBieG:ydH;zbZMh[2G3c3XkJIJ6KFeVUEG= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV{NEK0Okc,OjV3MkSyOFY9N2F-
MDA-MB-231 MWPDfZRwfG:6aXPpeJkhSXO|YYm= MWSxNO69VQ>? NG\VZ2UycA>? M4jzTIF2\22nboTzJINmdGxiZHXheIgh[W[2ZYKgR2hWSSC2cnXheI1mdnR? NIfM[5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWyNVUxOSd-MkW1NlE2ODF:L3G+
MKN28 NHf1SGxCeG:ydH;zbZMhSXO|YYm= NVzkboNoOTEQvF2= NXrpfolmOzCvaX6= M3y3WWROW09? Mlu3bY5pcWKrdIOgWG5HNc7zIIDseZMhS0i[LXnu[JVk\WRiYYDvdJRwe2m| M3z3dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUGzPVYxLz5{NUWxN|k3ODxxYU6=
HL-60 M{m3RWN6fG:2b4jpZ4l1gSCDc4PhfS=> MUexNFDPxE1? NGLRUncycA>? NXrDR3FYTE2VTx?= M2fXNOKhcW2ycn;2[ZMhfmmjYnnsbZR6KG:oIGTDUmF{NXS{ZXH0[YQh[2WubIO= M1jBW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUCyPVMzLz5{NUWwNlk{OjxxYU6=
AGS MoP2RZBweHSxc3nzJGF{e2G7 M4\4TlEx|ryP NXfhfGhOOWh? MY\wdoV3\W62czDjeZJkfW2rbj3pcoR2[2WmIHPs[YF3[WenIH;mJINie3Cjc3WtN{whNThuIHHu[EAuQSCycn;0[Ylvew>? NGHa[5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlIyPCd-MkW0PVIzOTR:L3G+
A549  M4TqWWFxd3C2b4Ppd{BCe3OjeR?= NU[wcGdqOTEQvF2= MUWyOIg> MnS3Zoxw[2u|IFTNRXMucW6mdXPl[EBkdGWjdnHn[UBw\iClYYPwZZNmNTNiYX7kJHBCWlBiYX7kJIFxd3C2b4TpZ{Bk\WyuIHTlZZRp NFLZRXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOFk5QSd-MkW0N|Q6QDl:L3G+
INS-1 MUDBdI9xfG:|aYOgRZN{[Xl? MVm1NO69VQ>? MXK2bC=> NGXub3JFVVOR MXXk[YNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[g[YFzdHliYYDvdJRwfGmlIHPlcIx{yqB? MnvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{C4PVcoRjJ3NEOwPFk4RC:jPh?=
Primary hepatocytes  NH7DPINCeG:ydH;zbZMhSXO|YYm= MmPnOVDPxE1? NHPmcWkyQGh? NVXTN5RrcW6qaXLpeJMh[XCxcITvd4l{KG:oIHjldIF1d2O7dHXzJIlv\HWlZXSgZpkhSWO2IFSgZY5lKFSQRj5OtS=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRyN{WzPEc,OjV2MEe1N|g9N2F-
L929-A  NHflV5pEgXSxdH;4bYNqfHliQYPzZZk> NEWy[2czOMLizszN MYOyOIg> MYXpcohq[mm2czDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGh? NWL5clZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzPVg2PDBpPkK1N|k5PTRyPD;hQi=>
L929-N NWGxV5RsS3m2b4TvfIlkcXS7IFHzd4F6 MVOyNOKh|ryP Mlz0NlRp MXflcohidmOnczDk[YF1cCC4aXGgZZV1d2O{aX7lJHRPTs7zIIDyc4R2[3Srb36= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7OEW0NEc,OjV|OUi1OFA9N2F-
Cytotoxicity Assay NXXodnhTS2WubDDWbYFjcWyrdImgRZN{[Xl? M3L3flIx|ryP NXjVUXZXPDiq NY[2SYp{eHKndnXueEBOUE2GLXnu[JVk\WRiY3XscEBl\WG2aB?= MnG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OUKxNVYoRjJ3M{myNVE3RC:jPh?=
COS7 MonjT4lv[XOnIFHzd4F6 NVniT2g6OTEQvF2= M4\XXFI1cA>? NF3reHZqdmO{ZXHz[ZMh[2G|cHHz[UA{NzdiYXP0bZZqfGmncx?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN6NECyOUc,OjV|OESwNlU9N2F-
COS7 M2jPWGFxd3C2b4Ppd{BCe3OjeR?= NH7uZmQyOM7:TR?= MmfUOFhp NXWxZpVNeGG{dHnhcIx6KHC{ZY\lcpRm\CCIQ{GwNU1qdmS3Y3XkJINmdGxiZHXheIg> MnT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OESwNlUoRjJ3M{i0NFI2RC:jPh?=
A375 MW\BdI9xfG:|aYOgRZN{[Xl? NWLkbHo4OzEQvF2= MXWybC=> MoKydJJmfmWwdIOgeIhmKGS{dXetbY5lfWOnZDDQRXJRKGOuZXH2ZYdm MnTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|N{[xNVUoRjJ3M{e2NVE2RC:jPh?=
A549 Mnz4RZBweHSxc3nzJGF{e2G7 M4rmc|Ux|ryP NGDhOFIzPGh? MoPmdoV3\XK|ZYOgdoljd3OxbXWgZolw\2WwZYPpd{BidmRiYYDvdJRwe2m|IHPheZNm\CCkeTCgR4hqcm:wZ3Thci=> NXmw[I12RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOFk4QDFpPkK1N|Q6PzhzPD;hQi=>
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A375 MUDBdI9xfG:|aYOgRZN{[Xl? M17FSlIx|ryP NFruZZgzPGh? NHT2[4Nz\X[ncoPld{BlcWOrdILpco9v\SCELXnu[JVk\WRiYYDvdJRwe2m| MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7OUGxNUc,OjR4OUmxNVE9N2F-
HDPC MV7BdI9xfG:|aYOgRZN{[Xl? MnGyOVDDvU1? MmTGNlRp NVvRWGwxcW6qaXLpeJMhVk9vaX7keYNm\CCjcH;weI9{cXN? M2LWUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkO0OVk{Lz5{NE[zOFU6OzxxYU6=
A549 MkjhRZBweHSxc3nzJGF{e2G7 NVO1XGVnPTEEtV2= MYOybC=> MoHRSG1UVw>? NYPwTZE4eHKndnXueJMhfGinIHj5dI9lcXCub3nkJGRPSSClb370[Y51KHCqYYPlJIlv\HWlZXSgZpkh[2WyaHHsc4Npem:vaX6= MkHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OEixN|UoRjJ2NUi4NVM2RC:jPh?=
 BV-2 MULBdI9xfG:|aYOgRZN{[Xl? NWfBSJlQOjEQvF2= MVyybC=> M3[0O5N2eHC{ZYPz[ZMhXVZvaX7keYNm\CClaILvcYF1cW5iZYj0dpV{cW:wIHHu[EBlcWyjdHnvckBw\iC2aHWgcpVkdGWjcjDlcpZmdG:yZR?= NFn6Vpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW1PFEyQCd-MkS1OVgyOTh:L3G+
HT-29  MVvBdI9xfG:|aYOgRZN{[Xl? NXHzWmlkPTEQvF2= M{LoZ|Q5cA>? MoXRZoxw[2u|IITo[UBieG:ydH;zbZMhcW6mdXPl[EBjgSCtYXXtdIZmem:u Ml:yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NEmxO|UoRjJ2NUS5NVc2RC:jPh?=
A549 M2riXGFxd3C2b4Ppd{BCe3OjeR?= MoWwOe69VQ>? NHzCXmEzPGh? M4HLcpN2eHC{ZYPz[ZMhPmYEoDjCT|ExODRyKT3pcoR2[2WmIHHwc5B1d3Orc9Mg NHjoSY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWyPVg4OSd-MkS1Nlk5PzF:L3G+
MIA-PaCa-2 NV63SoVHSXCxcITvd4l{KEG|c3H5 M3zKRVI1cA>? NGjxWIRjdG:la4OgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[U0{KGmwZIXj[YQh[nliYn;0bEBCNTR2M{[1OEBidmRicHHjcIl1[XinbB?= NYX3PJRsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVA6QTJpPkK0OVExQTl{PD;hQi=>
Caki-1 NFnTc2NCeG:ydH;zbZMhSXO|YYm= NIjpOY0zOM7:TR?= M{fOelFp NITRd5VqdmirYnn0d{BUS1BvaX7keYNm\CCjcH;weI9{cXN? NWTMcI1PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NFQ3QDFpPkK0OVA1PjhzPD;hQi=>
CLL MVrBdI9xfG:|aYOgRZN{[Xl? MVmyOe69VQ>? M2joNVFp MXTwZZJ1cWGubImgZoxw[2u|IF3MUlIzOzhvaX7keYNm\CClZXzsJIFxd3C2b4Ppdy=> MknJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2Nke2N|QoRjJ2NE[3OlM1RC:jPh?=
SGC-7901 M2XCXGFxd3C2b4Ppd{BCe3OjeR?= Mn3SNVDPxE1? Mn;1NlRp MoDNdJJwdW:2ZYOgeIhmKEOJSVmtbY5pcWKrdHXkJINmdGxiZ4Lve5RpKGmwIGPHR{04QTBzIHPlcIx{ MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUS0PFgoRjJ2NEW0OFg5RC:jPh?=
k1735 MYLBdI9xfG:|aYOgRZN{[Xl? NI\XeHEzOOLCid88cS=> MXW0bC=> Mn\JbY5pcWKrdIRCpHNidG2xbnXscIEucW6mdXPl[EBieG:ydH;zbZM> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3MUGxOkc,OjR2NUGxNVY9N2F-
HaCaT  Mn3qRZBweHSxc3nzJGF{e2G7 NH62VmcyODEQvF2= NGi0bo0ycA>? M{fDXoJtfW62czDVWmIucW6mdXPl[EBieG:ydH;zbZMhcW5iSHHDZXQh[2WubIO= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3Nkm5O{c,OjR|NU[5PVc9N2F-
podocytes NH[4OGJCeG:ydH;zbZMhSXO|YYm= NWPQcINTOjByzszN M1ntbVZp MnrtbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhOyx2LVTHSS=> NI\jOYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOzO|c4Pyd-MkSzN|c4Pzd:L3G+
 KB MontRZBweHSxc3nzJGF{e2G7 MV:1NO69VQ>? MnjRNlRp M1jZWolvcGmkaYTzJGxq[29vQT3pcoR2[2WmIHPhd5Bie2VvMzDhcoQhWEGUUDDhZ5RqfmG2aX;u M2HSOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{O3OFkzLz5{NEOzO|Q6OjxxYU6=
 HepG2  M33yVmFxd3C2b4Ppd{BCe3OjeR?= MkLqNVDPxE1? NYflWXVwOjSq MoK4doVlfWOnczD2bY1mdnSrbjDjcIVifmGpZTDjZZV{\WRiYomgUHBU MkntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkW4NVYoRjJ2M{K1PFE3RC:jPh?=
fetal rat lung fibroblasts  NVPnXWlXU2mwYYPlJGF{e2G7 MnzaPFDPxE4EoB?= Mm\NNYg> NYTtVJJEcW6qaXLpeJMhS2G|cHHz[U0{KGGldHn2bZR6yqB? NXftelFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVQyOzVpPkK0N|E1OTN3PD;hQi=>
PDL fibroblasts NHLSXHZMcW6jc3WgRZN{[Xl? NIjESIg5OM7:TdMg NIj5VVkycA>? NHzrVlVqdmirYnn0d{BE[XOyYYPlMVMh[WO2aY\peJnDqA>? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzNEGzOUc,OjR|MUSxN|U9N2F-
MB49 NYG2PIVISXCxcITvd4l{KEG|c3H5 M17OelIx|ryP NV63XVA3OWh? M17wTpJmfmW{c3XzJGNONWmwZIXj[YQh[2WubDDk[YF1cA>? NWHXeXZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFI1OzNpPkK0NlgzPDN|PD;hQi=>
5637 MkSxRZBweHSxc3nzJGF{e2G7 M1S2WFIx|ryP MX6xbC=> MmnZdoV3\XK|ZYOgR20ucW6mdXPl[EBk\WyuIHTlZZRp NUDjXHRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFI1OzNpPkK0NlgzPDN|PD;hQi=>
A549  NHmwWoxCeG:ydH;zbZMhSXO|YYm= MX6xNFDPxE1? NFL5Z4g1QGh? M3r6fJN2eHC{ZYPz[ZMhfGinIHHwc5B1d3OrczDjZZV{\WRiYomgdIlx\XKrbnZCpC=> NVywV5RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|IzODFpPkK0NlczOjBzPD;hQi=>
MCC-2 NUDyclJRSXCxcITvd4l{KEG|c3H5 NFjK[ZQzOCEQvF2= M2jwe|Zp MWjEUXNQ MlPvZoxw[2u|IHnu[JVk\WRiYomgd5RifXKxc4Dvdolv\SCjcH;weI9{cXNiY3XscEBl\WG2aB?= MnTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NkK2OVgoRjJ2Mk[yOlU5RC:jPh?=
ADF MWfBdI9xfG:|aYOgRZN{[Xl? NHjZNFY1OM7:TR?= M1GwPVZp NIPySnBqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClaHXtc5Rp\XKjcImgdIx2eyCcblPsNi=> NXnq[3loRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlgzOzJpPkK0NlI5OjN{PD;hQi=>
 U373  NGW1WGRCeG:ydH;zbZMhSXO|YYm= MlSzOFDPxE1? NFfSN4w3cA>? NFjYUGNqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClaHXtc5Rp\XKjcImgdIx2eyCcblPsNi=> MkHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
RKO-HIPK2i MWLBdI9xfG:|aYOgRZN{[Xl? M4TG[FQx|ryP NFuxd2s3cA>? MlvEbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkh[2inbX;0bIVz[XC7IIDseZMhYm6FbEK= MknjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
HEC-1B NWC4dI5TSXCxcITvd4l{KEG|c3H5 NFu1S3UzOM7:TR?= MXmxbC=> NWfQNYl4emWmdXPld{BVWC2rbnT1Z4VlKGGyb4D0c5Nqe8LiY3HzdIF{\S1|IHHu[EBk[XOyYYPlMVk> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzM{O1PEc,OjR{MUOzOVg9N2F-
4T1 Mn70R4VtdCCYaXHibYxqfHliQYPzZZk> MYKyMlUuOTEQvF2= NHnvdnU1cA>? MUXEUXNQ NXnHfFlwemW|Y4Xld{B1cGViY4n0c5RwgGmlaYT5JI9nKDSWMTDj[YxteyCrbnT1Z4VlKGK7IGPQSHQhcW5iYTDjc45k\W62cnH0bY9vKGSncHXu[IVvfCCvYX7u[ZI> M4nKelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkC2NVYyLz5{NEKwOlE3OTxxYU6=
OS  Mn[yRZBweHSxc3nzJGF{e2G7 MWKyNO69VQ>? MXS0bC=> M2rzNolvcGmkaYTzJJRp\SClZXzsJIRm[XSqIHPheZNm\CCkeTDQSHQ> Mn\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MES5N|coRjJ2MkC0PVM4RC:jPh?=
RPE MnrnRZBweHSxc3nzJGF{e2G7 NXvqRYV{OTByzszN MnzZOFhp M13XOZBienSrYXzsfUBqdmirYnn0d{B1cGViY3HsdIFqdi1zIHHu[EAuOiCjY4TpeoF1cW:wIHHzJJdmdGxiYYOgeIhmKGOjc4Dhd4Uh[WO2aY\heIlwdg>? M33TUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkCyNFUzLz5{NEKwNlA2OjxxYU6=
UD29a M3PyTmFxd3C2b4Ppd{BCe3OjeR?= MU[1NO69VQ>? M1;wO|I1cA>? MYnpcohq[mm2czD0bIUh[2WubDDk[YF1cCClYYXz[YQh[nliTmXUNy=> M4W0UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUmwOVc1Lz5{NEG5NFU4PDxxYU6=
SH-SY5Y  NI\jWIpCeG:ydH;zbZMhSXO|YYm= MXOxNO69VQ>? MXeyOIg> NWftVXBy[XS2ZX71ZZRmeyClYYPwZZNmKGGldHn2ZZRqd25iYX7kJINmdGxiZHXheIghcW6mdXPl[EBjgSCKTlWtNmRI NF7tcpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0OVQ3Oyd-MkSxOFU1PjN:L3G+
HeLa  NV;tSZF[SXCxcITvd4l{KEG|c3H5 M2LNRVExNzJyzszN MlfLNlRp MVLpcohq[mm2czD0bIUh[WO2aY\peJkhd2ZidHjlJI1icm:{aYT5JI9nKHSqZTDt[Y1j\XK|IH;mJJRp\SClYYPwZZNmNW[jbXnsfS=> M1zOWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO3NlY3Lz5{NEGzO|I3PjxxYU6=
SCCVII  MWnBdI9xfG:|aYOgRZN{[Xl? NEHvV3IzPc7:TR?= NVXwdoFUOWh? NHr0b5ZqdmirYnn0d{B1cGViY3XscEBscWyuaX7nJIFnfGW{IHThd4F1cW6rYh?= M4LwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK2OFY1Lz5{NEGyOlQ3PDxxYU6=
RAW 264.7 MX7BdI9xfG:|aYOgRZN{[Xl? MYeyNO69VQ>? MWSxPIg> MWHpcoNz\WG|ZYOgUGM{NUmLL98yMYFkfGmwIILheIlwKGOxbYDhdoVlKHSxIFXDWHYuVU:VIITy[YF1dWWwdDDvcox6 M3m4O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUG2O|A4Lz5{NEGxOlcxPzxxYU6=
A549  NHWwXJRCeG:ydH;zbZMhSXO|YYm= NEfvbZQzNjYQvF2= MoHONlRp M1y2eIRm[3KnYYPld{Bwemmmb37pck1qdmS3Y3XkJIF2fG:yaHHnfUBieyC5ZXzsJIFv\CCOb4PzJI9nKM7Wz5jtJIFte29ib3PjeZJz\WRiZIXybY5oKGG3dH;wbIFocWNicILvZ4V{ew>? M{fUOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCyOVIzLz5{NEGwNlUzOjxxYU6=
UM-SCC-10A  NFXJTHdCeG:ydH;zbZMhSXO|YYm= NGH5Zoc2OML3TR?= MlvJNog> NF30d2Nz\WS3Y3XzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGirZ3it[I9{\SCrc3;hcIFvfG:uYXP0c45m MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3OVMoRjJ2MEm4O|U{RC:jPh?=
BGC-823 MmfSRZBweHSxc3nzJGF{e2G7 NF;kbnAyOCEQvF2= NFzmOGwzPGh? NHntZlRz\WS3Y3XzJJRmfHKjbnTybY5mNWmwZIXj[YQh[XCxcITvd4l{yqB? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEWxNUc,OjRyOUi1NVE9N2F-
Nalm-6  MYTBdI9xfG:|aYOgRZN{[Xl? MWmyNO69VQ>? M2\zc|Rp NVLW[5BzcW6qaXLpeJMh[2G|cHHz[U05KGGwZDDjZZNx[XOnLUOgZYN1cX[jdHnvckBidmRiUFHSVE0yKGOuZXH2ZYdm NHHUU5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECzPVk3Pyd-MkSwN|k6Pjd:L3G+
KHOS  NWjMeXlySXCxcITvd4l{KEG|c3H5 NUfqRppnPDEQvF2= NVG2SndKOjSq NEDtT4tjdG:la4OgeIhmKGmwY4LlZZNm\CClbHXheoFo\SCxZjDjZZNx[XOnLUOgZY5lKFCDUmCgbY5lfWOnZDDifUBDSk2GM9Mg NXXqU5cyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlU{PjFpPkK0NFI2OzZzPD;hQi=>
G292 M4\PO2Fxd3C2b4Ppd{BCe3OjeR?= M1rNR|Qx|ryP MnrZNlRp Mn\CZoxw[2u|IITo[UBqdmO{ZXHz[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgRmJOTDQEoB?= M1GwdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK1N|YyLz5{NECyOVM3OTxxYU6=
MG-63 M2DhXmFxd3C2b4Ppd{BCe3OjeR?= M{LtTVQx|ryP M3vIb|I1cA>? MoLDZoxw[2u|IITo[UBqdmO{ZXHz[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgRmJOTDQEoB?= NGCyb249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyOVM3OSd-MkSwNlU{PjF:L3G+
EBV-transformed B cells MmfERZBweHSxc3nzJGF{e2G7 M3jLflIx|ryP NWHXbHZnOmh? MX7EUXNQ NHvuRW5jdG:la4OgZY51cS2FREiwJIFv\CCjboTpMWNFQDZiYX70bYJw\HlvaX7keYNm\CCjcH;weI9{cXN? NVHyPGJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNFg3OjhpPkK0NFA5PjJ6PD;hQi=>
IM-9 B M{K2RmFxd3C2b4Ppd{BCe3OjeR?= M2PCVVIx|ryP M{TJfVJp MY\EUXNQ MkjhZoxw[2u|IHHueIkuS0R6MDDhcoQh[W62aT3DSFg3KGGwdHnic4R6NWmwZIXj[YQh[XCxcITvd4l{ MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDByOE[yPEc,OjRyMEi2Nlg9N2F-
CNE1 NUDhfFdvSXCxcITvd4l{KEG|c3H5 NYDMeGh1OjEQvF2= NVixVVd6PDiq MV3icI9kc3NiTFutRU1qdmS3Y3XkJIFxd3C2b4Ppdy=> MnHsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7OEWwNlkoRjJ|OUi1NFI6RC:jPh?=
CNE2 NF\MZYNCeG:ydH;zbZMhSXO|YYm= MkTtNlDPxE1? NGi3NpI1QGh? MlHsZoxw[2u|IFzLMWEucW6mdXPl[EBieG:ydH;zbZM> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl6NUCyPUc,OjN7OEWwNlk9N2F-
HL-60 M4Tsb2Fxd3C2b4Ppd{BCe3OjeR?= NEnJbGo2OML3TR?= MX[xbC=> MX;EUXNQ NUnmOWp4cW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliQlGxOFU> NH[1eGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0PFc2OSd-MkO5OFg4PTF:L3G+
L929 NUmxUpFWSXCxcITvd4l{KEG|c3H5 MUixMlI26oDVNdMg{txOyqB? NG\FZYwzPGh? NVjNbYJD[XWpbXXueJMhXE6IzsGtbY5lfWOnZDDu[YNzd3C2b4Ppd{BidmRiYYX0c5Bp[We7 NFnvT5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0NVc3QSd-MkO5OFE4Pjl:L3G+
Jurkat MV3D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmHQNu69VQ>? M3;rc|Rp MkXndoV3\XK|ZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKM7{LXPheIVvcW5iZHXjdoVie2ViYomgTHMuSVOD MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh7NkC2NUc,OjN6OU[wOlE9N2F-
RAW264.7  NH3Ec2tCeG:ydH;zbZMhSXO|YYm= NVPuZXc5OTBizszN M2PWfVI1cA>? NVLveHVw\GWlcnXhd4V{KHSqZTDhdI9xfG:|aYOgbY5lfWOnZDDifUBISQ>? NHKyUVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyNFIxOyd-MkO4NlAzODN:L3G+
A431  M3LiUGtqdmG|ZTDBd5NigQ>? MXy0NO69VQ>? Mni2Nog> MUnicI9kc3NiZnnz[ZRqdi2rbnT1Z4VlKGOuZXH2ZYdmKG:oIHPhd5Bie2W|IHHu[EBRSVKS MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyMEC1PEc,OjN6MECwOVg9N2F-
Jurkat M{HSXGNmdGxiVnnhZoltcXS7IFHzd4F6 NF:5XW0zPS1zMEFOwG0> MXS2bC=> Mme5bY5pcWKrdIOgfk1HSS2FTVutbY5lfWOnZDDj[YxtKGSnYYTo MljlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{O1N|IoRjJ|OUOzOVMzRC:jPh?=
COS-7  MUjGeY5kfGmxbjDBd5NigQ>? NUXtcnVsPTEEtV2= NIH2cWYzPGh? MV7h[oZm[3S|IITo[UBxem:lZYPzbY5oKG:oIFHUUlF{KHerdHigdI9tgVFidILhZ5R{ NFTCbnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzN|IxQCd-MkO5N|MzODh:L3G+
CAL27 MUjBdI9xfG:|aYOgRZN{[Xl? MYexNEDPxE1? MknpNog> NVX6OFhCe3WycILld5NmeyCFdYKtUnB{NXKnZIXj[YQhfmmjYnnsbZR6KGK7IIXwJJRwKDlyJR?= M4jnUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUG3N|k3Lz5{M{mxO|M6PjxxYU6=
THP-1  NHrFZ5VCeG:ydH;zbZMhSXO|YYm= MmrUNVAuPTEQvF2= MoTwNog> NFPGV|ZqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSC2cnnweI9tcWSn NHjyV3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwNFI6QSd-MkO5NFAzQTl:L3G+
K562 NUOxOWhNSXCxcITvd4l{KEG|c3H5 M3\kVFIx|ryP NYLicHFSPDiq Mmj1bY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhPC2PVR?= M2LOe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe2PFI3Lz5{M{i3OlgzPjxxYU6=
HL-60 MYPBdI9xfG:|aYOgRZN{[Xl? NFfYfpEyODEQvF2= M4frdVI1cA>? NXXsVJRLcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliYXLp[ZRidmViZHn0[ZJx\W6nc9Mg M1qxcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OE[1O|c5Lz5{M{i2OVc4QDxxYU6=
HeLa  MWjBdI9xfG:|aYOgRZN{[Xl? NIrHOm0zOM7:TR?= Ml3MNog> M1XzbolvcGmkaYTzJGROVVBiaX7keYNm\CCjcH;weI9{cXN? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4M{m2Okc,OjN6NkO5OlY9N2F-
U251 NYKwfW1WSXCxcITvd4l{KEG|c3H5 M3zQU|Ix|ryP NXfGZmxIOjSq NWLLUJZ5cW6qaXLpeJMh[2G|dHnjbY4hcW6mdXPl[EBIOi:PIIDoZZNmKGG{cnXzeEBidmRiYYDvdJRwe2m| MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzNkixOkc,OjN6MU[4NVY9N2F-
HL-60 MmfQRZBweHSxc3nzJGF{e2G7 MV[1NOK2VQ>? M4[zUFRp MWnicI9kc3NidHjlJINt\WG4YXflJI9nKGOjc4Dhd4UuOyxiLUmsJIFv\CCSQWLQJIlv\HWlZXSgZpkhPnN? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyNEewOkc,OjN6MES3NFY9N2F-
Ec-109 NWrSOohHSXCxcITvd4l{KEG|c3H5 M{TvNVExKM7:TR?= NEH0Umc1QGh? NUfTS3pCemWycnXzd4V{KFCHLX3l[IlifGWmIFXjMVExQSClZXzsJIFxd3C2b4Ppdy=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6Mk[0NUc,OjN5OEK2OFE9N2F-
RKO NFP6VG1E\WyuIG\pZYJqdGm2eTDBd5NigQ>? M4m1UFExKM7:TR?= NF3Wc24zPGh? NH7mOZNqdmirYnn0d{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliY3H1d4VlKGK7IFTBRi=> MmXFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NUiwOlQoRjJ|N{W4NFY1RC:jPh?=
T98G MVrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M1TFdFEuOTByzszN MoDRNlRp NHXhW4tqdXC{b4\ld{Bk\WyuII\pZYJqdGm2eTDjc5Rz\WG2bXXueEB4cXSqIGTT MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd4OUK3OUc,OjN5NkmyO|U9N2F-
Jurkat MUXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M{PiUVExOC1{MEFOwG0> M1W1[FI1cA>? NXHNXpp[cW6qaXLpeJMhUGGDNDDpcoR2[2W|IHHwc5B1d3OrczDk[ZBmdmSnboSgc4Yh[2:wY3XueJJifGmxbtMg NF64T5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ezNlQ5OSd-MkO3N|I1QDF:L3G+
Molt-3  M3G0cmFxd3C2b4Ppd{BCe3OjeR?= NGrj[Vc2OM7:TR?= NF;6RYczcA>? MljVdoVlfWOnczDt[YxifG:waX6tbY5lfWOnZDDhdI9xfG:|aYO= NHHpVIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{eyOVAyOyd-MkO3NlUxOTN:L3G+
hMSC12 MkHTRZBweHSxc3nzJGF{e2G7 NU\D[YdGOTByzszN MXy0[C=> M2T1eYlvcGmkaYTzJI9{fGWxZ3XubYMh[3WudIXy[U1qdmS3Y3XkJINmdGxiZHXheIgh[W6mIHPhcINq\mmlYYTpc44> MlTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NUe4NlIoRjJ|NkW3PFIzRC:jPh?=
HM7  M{iwRWFxd3C2b4Ppd{BCe3OjeR?= MkjkNlDPxE4EoB?= MVyxbC=> MXPicI9kc3NiYYDpZ5Vt[XKnbjDBJIFk\XSjdHWtbY5lfWOnZDDjZZNx[XOnLUOgZYN1cX[jdHnvckBidmRiUFHSVEBkdGWjdnHn[S=> MnXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OEO0NVIoRjJ|NUizOFEzRC:jPh?=
Hep-2  MV;BdI9xfG:|aYOgRZN{[Xl? NYfGdFN3OjEQvF5CpC=> M{C1TlI1cA>? MXzEUXNQ Mlq4ZYxt\X[rYYTld{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliaX7keYNm\CCkeTDzbYxq[mmwaX6= M2\w[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUiwNFMzLz5{M{W4NFA{OjxxYU6=
HSCs M4rRbGFxd3C2b4Ppd{BCe3OjeR?= NVSz[|JKPTEQvF2= M{P4d|I1cA>? NEG0W|ZFVVOR NH7ybHJqdmirYnn0d{BvcWyxdHnubYIucW6mdXPl[EBFVkFiZHHtZYdmKGmwZHnjZZRm\CCkeTDQRXJRKGOuZXH2ZYdm M{jrSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm5PFc1Lz5{M{S5PVg4PDxxYU6=
HL-60  MmXOR5l1d3SxeHnjbZR6KEG|c3H5 NUf4V4FSOjEQvF5CpC=> NYPhT3Q3OjSq NUTOVm96emWmdXPld{B1cGViY4n0c5RwgGmlIHXm[oVkfCCxZjDFdpk2 MnjwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUS0PFAoRjJ|NEm0OFgxRC:jPh?=
HA NHS2WmZCeG:ydH;zbZMhSXO|YYm= NX3GWm1YPTEQvF2= NEfY[o4zPGh? MULzeZBxemW|c3XzJJRp\SClbHXheoFo\SCxZjDQRXJRKGGwZDDjZZNx[XOnLUOsJE04NCCjbnSgMVkhcW6mdXPl[EBjgSCkb4L0[ZpwdWmkL2PBTGE> NV;L[5Q{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0O|U6PTZpPkKzOFc2QTV4PD;hQi=>
C666-1 MX7BdI9xfG:|aYOgRZN{[Xl? NHvZOFU2OM7:TR?= NUTVd5NCOjSq M3;zRpN2eHC{ZYPz[ZMhfGinIHPs[YF3[WenIH;mJHBCWlBiYX7kJINie3Cjc3WtN{whNTduIHHu[EAuQSCrbnT1Z4VlKGK7IHLvdpRmgm:vaXKvV2FJSQ>? NWXGOZJYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0O|U6PTZpPkKzOFc2QTV4PD;hQi=>
Hepa1-6 M3nPZmFxd3C2b4Ppd{BCe3OjeR?= Mn3tOVDPxE1? M1G4eFJp MUDpcohq[mm2czDjeZJkfW2rbjDhcoQhemW|dnXyZZRzd2xvaX7keYNm\CCjcH;weI9{cXN? M4fYelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NES2O|U{Lz5{M{S0Olc2OzxxYU6=
PLC/PRF/5c NHT5bpdCeG:ydH;zbZMhSXO|YYm= NV7FNWhCPTEQvF2= NUH4bFhkOWh? MWTwdoV3\W62czDhdI9xfG:|aYOgeJJq\2encnXkJIJ6KEOLTh?= NFqwR|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SzPFgzPCd-MkO0N|g5OjR:L3G+
HCT116 M2Ht[mFxd3C2b4Ppd{BCe3OjeR?= NGrKd|YyODEQvF2= MXixMlVp M1OwOYlvcGmkaYTzJG9{NWmwZIXj[YQh[2WubDDk[YF1cA>? M{D1bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{S0N|U{Lz5{M{e0OFM2OzxxYU6=
HCEC MlW4SpVv[3Srb36gRZN{[Xl? NEHIZ5k2OM7:TR?= MX63Nog> NUfoW41NTE2VTx?= NFTUOnFz\XO2b4Lld{Bw\iC2aHWgco9zdWGuIFjDSWMheGinbn;0fZBm Moi5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NEKwNVEoRjJ|N{SyNFEyRC:jPh?=
Primary rat cerebral cortical neurons MWPBdI9xfG:|aYOgRZN{[Xl? NXnkfY9rOTByzszN MUWxbC=> MlLqdJJmfmWwdIOgR4QucW6mdXPl[EBieG:ydH;zbZMh[W6mIHPlcIwh\GWjdHi= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2MUOxO{c,OjN5NEGzNVc9N2F-
MDA-MB-231  M{DaOmFxd3C2b4Ppd{BCe3OjeR?= NYjBdG5lOjYQvF2= M3X1blI1cA>? Mmq1ZYJzd2ejdHXzJIN6fG:2b4jpZ4l1gSCjbnSgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[U0{KGGwZDDQRXJRKGmwZIXj[YQh[nliUnez M1f3[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{O3OVQ1Lz5{NUOzO|U1PDxxYU6=
NLRP3-Tet-on-MC/9  NUC1PYhKSXCxcITvd4l{KEG|c3H5 Mon3NVAuPDEQvF2= MV6xNog> MW\heJRmdnWjdHXzJJRp\SClZXzsJIRm[XSqIHPheZNm\CCkeTDDRXBUNWG|c3;jbYF1\WRiTlzSVFMhdXW2YX70d{BqdiC2aHWgWIV1NW:wIIP5d5RmdQ>? M{HXdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{CzN|g6Lz5{M{ewN|M5QTxxYU6=
KNS42  Mon4RZBweHSxc3nzJGF{e2G7 MYO1NO69VQ>? MkK5NYg> NVj3TJJYemWmdXPld{Bk\WyuIHTlZZRpKGGwZDDjc41xdGW2ZXz5JIFjd2yrc3jl[EBk[XOyYYPlJFMwPyCjY4Tpeol1gSCrbjDy[ZNxd26|ZTD0c{BCSlRvMk[zM|JFTy:vZYTmc5JucW5iY3;tZolv[XSrb36= MnzXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OUGxOFUoRjJ|NkmxNVQ2RC:jPh?=
MCF-7  NHLCcWFCeG:ydH;zbZMhSXO|YYm= MkXyNlDPxE4EoB?= NY\VTItXPzKq NH2y[plqdmirYnn0d{BmeXWxbD2gZY5lKDRvT1jUMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NGfaSWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[3OVY1Oyd-MkO2O|U3PDN:L3G+
hCMEC/D3  MVPBdI9xfG:|aYOgRZN{[Xl? MnPRNlXPxE1? MU[3Nog> NUL5UopVemWmdXPld{BNfHiDIHnu[JVk\WRiYYDvdJRwe2m| MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ4NUG5PEc,OjN4NkWxPVg9N2F-
Jurkat MWTBdI9xfG:|aYOgRZN{[Xl? M{i4flEzNjVvNUFOwG0> NHHJRWQycA>? NUiyOmJN\G:|ZTDk[ZBmdmSnboTsfUB{fXCycnXzd4V{KFOSSD3pcoR2[2WmIGDhdk01KGOuZXH2ZYdmNCCSQWLQJINt\WG4YXflMEBFVkFiZoLh[41mdnSjdHnvckwh[W6mIHzvd5Mhd2ZidnnhZoltcXS7 NYTjfG9VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFI6PzZpPkKzOFQzQTd4PD;hQi=>
CNE-1 MmLaRZBweHSxc3nzJGF{e2G7 MnHVNlDPxE4EoB?= NV22N41tOjSq MXzpcohq[mm2czDSRWQxODFvaX7keYNm\CClZXzsJIRm[XSq NIrocmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SyOlg2OCd-MkO0NlY5PTB:L3G+
HONE-1 NEPBeHlCeG:ydH;zbZMhSXO|YYm= MmSxNlDPxE4EoB?= M2LN[FI1cA>? MXrpcohq[mm2czDSRWQxODFvaX7keYNm\CClZXzsJIRm[XSq MkL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2Mk[4OVAoRjJ|NEK2PFUxRC:jPh?=
astrocytes cell MmfFRZBweHSxc3nzJGF{e2G7 MVO0NO69VQ>? NXXQfplsPmh? M{PCdZJm\HWlZYOg[YFzdHliYYDvdJRwe2m|IHnu[JVk\WRiYomgd5RifXKxc4Dvdolv\Q>? Mn;xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MUG3O|goRjJ|NEGxO|c5RC:jPh?=
U-937 MoTvRZBweHSxc3nzJGF{e2G7 MVGxNO69VQ>? MlXlN|BucW5? NGrjRY9xemW4ZX70d{BVVkZvaX7keYNm\CCwZXPyc5B1d3Orcx?= NFq5UJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxNFc1QCd-MkO0NVA4PDh:L3G+
MDA-MB-231 MWPBdI9xfG:|aYOgRZN{[Xl? MYKxNFDPxE1? M4XiclFp NWHEOGcycW6qaXLpeJMhe2Wwc3n0bZpifGmxbjD0c{BVWkGLTDD1dI9vKE2WRFig[I94di2{ZXf1cIF1cW:w Mn7kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEi0NlkoRjJ|NEC4OFI6RC:jPh?=
HeLa  MnjERZBweHSxc3nzJGF{e2G7 MlLMNVAx|ryP MVOybC=> MnfUZoxw[2u|IFrSV{0yPSCLbnT1Z4V{KEGyb4D0c5Rq[yCFZXzsJGRm[XSq NH\hbIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O0OFA1PSd-MkOzOFQxPDV:L3G+
Ec109 MWjBdI9xfG:|aYOgRZN{[Xl? MnOxNVDPxE1? NYDxT4pjPmh? M33pO4Jtd2OtczDhdI9xfG:2aXOgZ49u[mmwYYTpc44hd2ZiVHH0MXNu[WOQNzDhcoQhemGmaXH0bY9v Ml3kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|M{i1OlgoRjJ|M{O4OVY5RC:jPh?=
H460  NVrSPYFTSXCxcITvd4l{KEG|c3H5 MU[xNO69VQ>? M{nBdVZp MkfIZoxw[2u|IHHwc5B1d3SrYzDjc41jcW6jdHnvckBw\iCWYYStV41i[055IHHu[EBz[WSrYYTpc44> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN|OEW2PEc,OjN|M{i1Olg9N2F-
HeLa  NVzuZoNZSXCxcITvd4l{KEG|c3H5 NILq[3g2OM7:TR?= NYDXNYVIOS53aB?= NV;3dohF[WK{b3fheIV{yqCFaHzhcZllcWFvaX7keYNm\CCjcH;weI9{cXN? M2nQdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{CzPFA1Lz5{M{OwN|gxPDxxYU6=
SK-HEP1 NGDoNW9CeG:ydH;zbZMhSXO|YYm= NFTlNokyODEQvF2= NUL5fGFqOWh? MVLpcohq[mm2czDDdnQyKGGldHn2ZZRm\CClYYPwZZNmNTNuID23MEAuQCxiLUmsJIFv\CCyb3z5LGFFWC2{aXLvd4UqKHCxbInt[ZJie2V? NEDBXII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OwNlY2OCd-MkOzNFI3PTB:L3G+
QGY7701 NGH4dIJCeG:ydH;zbZMhSXO|YYm= MmrSNlXPxE1? NFrSOIMyNjWq NHyydFhqdmirYnn0d{Bi[2O3bYXsZZRqd25ib3[gd5VjNUdzIIDoZZNmKGmwZIXj[YQh[nliRF;YJEsheXWncnPleIlv M4S0T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkSwNFYyLz5{M{K0NFA3OTxxYU6=
HepG2 NH62XFBCeG:ydH;zbZMhSXO|YYm= MVWyNO69VQ>? MYGzNI1qdg>? M2f0NolvcGmkaYTzJJRp\SCnbnjhcoNm\CClZXzsJIRm[XSqIHL5JINwdWKrbnXkJJRz\WG2bXXueEBw\iCjcHnn[Y5qdiCjbnSgWHJCUUx? MnHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{MkSyN|koRjJ|MkK0NlM6RC:jPh?=
U87  MojVRZBweHSxc3nzJGF{e2G7 M4Hab|I2|ryP M{Hm[FJp NGnj[WVl\WO{ZXHz[ZMhcXOxbHnxeYlzcXSrZ3XubY4hMEmVTDmtbY5lfWOnZDDhdI9xfG:2aXOgZ4VtdCCmZXH0bEwh[nW2IH7veEBv\WO{b4TpZ{Bk\WyuIHTlZZRp NUHNUVY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyNlk3OjZpPkKzNlI6PjJ4PD;hQi=>
HSC-2 M4LEWWFxd3C2b4Ppd{BCe3OjeR?= NEPTOGEzPS93MN88US=> M{X3SVFp MXzpcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF3NUK0PEc,OjNzNUWyOFg9N2F-
HSC-4 NXO1O3NjSXCxcITvd4l{KEG|c3H5 MoXRNlUwPTEQvF2= NUK4OYoxOWh? MX\pcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF3NUK0PEc,OjNzNUWyOFg9N2F-
CL-1 NWq1ZYZjSXCxcITvd4l{KEG|c3H5 NWL3TmhoOjEQvF2= M2j0[FFp MoHiZoxw[2u|IF;QOFQ6KGmwZIXj[YQh[2WubDDk[YF1cA>? MkOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzM{G3PFIoRjJ|MUOxO|gzRC:jPh?=
MEL MnLDRZBweHSxc3nzJGF{e2G7 M2DhUFIx|ryP NVfNO5ZvOjSq MUXEUXNQ NGGzbJNqdXCjaYLld{BROlh5LXnu[JVk\WRiTVXMJINmdGxiYYDvdJRwe2m| MorWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUS4PFcoRjJ|MEG0PFg4RC:jPh?=
Bel-7402  MYXDZZNx[XOnIFHjeIl3[XSrb36gRZN{[Xl? NEDnW5A2OM7:TR?= MYCybC=> M1O3bIlvcGmkaYTzJHBETS2rbnT1Z4VlKGGwb3nrbZM> Mk\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEi3OFIoRjJ|MEC4O|QzRC:jPh?=
Eca-109 MnPtRZBweHSxc3nzJGF{e2G7 NF[0[GkzPc7:TR?= NWLBTJhwOzCvaX6= MlvIbY5pcWKrdIOgRmouSjFzLXnu[JVk\WRiY3XscEBl\WG2aB?= MkjtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyN{[5OlcoRjJ|MEe2PVY4RC:jPh?=
MEL Mn6wRZBweHSxc3nzJGF{e2G7 MV6yNO69VQ>? NVvjW|Y6OWh? NV\URpZbTE2VTx?= NUK2SHY5cW2yYXny[ZMhWDK[Nz3pcoR2[2WmIF3FUEBk\WyuIHHwc5B1d3Orcx?= Mlr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUS4PFcoRjJ|MEG0PFg4RC:jPh?=
Bel-7402  Ml[1RZBweHSxc3nzJGF{e2G7 MXO1NO69VQ>? NWX5eYs4Omh? MlXTbY5pcWKrdIOgVGNGNWmwZIXj[YQh[W6xaXvpdy=> NHq4fJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CwPFc1Oid-MkOwNFg4PDJ:L3G+
L929 MWHGeY5kfGmxbjDBd5NigQ>? NHi1TFkzNjYQvF2= M{O4[FFp MWjpcoNz\WG|ZYOgVmlROSCneIDy[ZN{cW:wIHHu[EBmgGGlZYLiZZRm\CCWTldOtU1qdmS3Y3XkJI1qfG:laH;u[JJq[WxiZInz[pVv[3Srb36gZY5lKFKRUzDwdo9lfWO2aX;u NEG5SlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CwNFUyQCd-MkOwNFA2OTh:L3G+
RCC  MkHBR4VtdCCYaXHibYxqfHliQYPzZZk> MkWzNVAx|ryP MXiyOIg> Mn:3doVkd3[ncnXzJJRp\SC4aXHibYxqfHlib3[gZ4VtdHNiZYjwc5Nm\CC2bzCxOYQuWEeMMh?= MlvMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OUG0PVQoRjJ{OUmxOFk1RC:jPh?=
NB2a/d1 NHvSSZFCeG:ydH;zbZMhSXO|YYm= MoDnNVAx|ryP MnPiO|Jp NVr4e3Fb[XS2ZX71ZZRmeyC|dHH1do9{eG:{aX7lMYlv\HWlZXSgZ4F{eGG|ZTDhZ5Rqfmm2eTygVGFTWCxiYX7kJJRifSClbHXheoFo\Q>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl6OEW0NUc,OjJ7OEi1OFE9N2F-
T cell MlHjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\1O2pROjVvMUCw{txO Mo\GNlRp MnrW[I9{\S2mZYDlcoRmdnSueTDpcohq[mm2ZXSgWEBk\WyuIIDyc4xq\mW{YYTpc44hdWWmaXH0[YQhfGi{b4XnbEB1cGViY3:td5RqdXWuYYTpc44hf2m2aDDhcpRqNUOGMzDhcoQh[W62aT3DSFI5 NGPlO409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm4NlU{QCd-MkK5PFI2Ozh:L3G+
K562 NHj1WJZCeG:ydH;zbZMhSXO|YYm= MYKxNFDPxE1? NYLRWpJzOWh? NGXsZ|NjdG:la4OgRYJvd2KjdnnzZ5VuyqCILXnu[JVk\WRiYYDvdJRwe2m| Ml\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{KzO|IoRjJ{OUeyN|czRC:jPh?=
Jurkat  Mkm1RZBweHSxc3nzJGF{e2G7 M1zZSlQx|ryP MV6xbC=> NYnFN2k5[WKxbHnzbIV{KE[jc1ytbY5lfWOnZDDjZZNx[XOnIHHjeIl3[XSrb36gZY5lKGOnbHyg[IVifGh? Mmi2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NEK3N|goRjJ{OUSyO|M5RC:jPh?=
BGC-823 NUW3N4FYSXCxcITvd4l{KEG|c3H5 NHjwfGQyODEQvF2= NXTSbG9nOWh? MW\wZZJ1cWGubImgdoV{[3WnczDj[YxteyCjZ3HpcpN1KGSjbXHn[UBw\iCmYXnkfoVqdg>? NF3QfXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlU1PSd-MkK5NlY2PDV:L3G+
Hep3B M4rV[GFxd3C2b4Ppd{BCe3OjeR?= NHW3boU2OM7:TR?= NIHBdmIycA>? NEL0SZRjdG:la4OgZZBweHSxc3nzJIlv\HWlZXSgZpkhUEWJQ4O= NFPzTJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyN|E2PCd-MkK5NlMyPTR:L3G+
LLC-PK1 MVnBdI9xfG:|aYOgRZN{[Xl? NGrD[ZIzOM7:TR?= NH;0[IYycA>? NHPIO|FxemW4ZX70d{BkcXOybHH0bY4ucW6mdXPl[EBl\We{YXTheIlwdiCxZjDBeIc2NCCkZXPsbY4uOSxiYX7kJGF1\zF{IIDyc5RmcW6| NW[xOIVxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4PVYxOzdpPkKyPFk3ODN5PD;hQi=>
A549 MXfBdI9xfG:|aYOgRZN{[Xl? NWO1PXJ[PTEQvF2= NWLobGJbOWh? MkPZZoxw[2u|IITo[UBDSUlvaX7keYNm\CCjcH;weI9{cXQEoB?= M3TnSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEi3NlE2Lz5{Mki4O|IyPTxxYU6=
SGC-7901  NFv6bZVCeG:ydH;zbZMhSXO|YYm= M3LJTlEx|ryP MWOyOIg> NEKwbGVqdmirYnn0d{DPui{Qsj3kbY1mfGi7bHHjdplte2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZPDqA>? NGLMVIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki0PFU6Pyd-MkK4OFg2QTd:L3G+
DM6 M3XuOGFxd3C2b4Ppd{BCe3OjeR?= M1jkOFExOM7:TR?= M1nBXlczcA>? MY\icI9kc3NiYn;0bEBGOkZvMTDhcoQhTTKIdIKtcYVlcWG2ZXSgZ5l1d3SxeHnjbZR6 NU[5d3h1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{OjhpPkKyPFI2OzJ6PD;hQi=>
MCF-7, MDA-MB-468, Caco-2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TN[lUx|ryP MWW0PIg> MkKybY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnubIljcXSrb36gbY5lfWOnZDDifUB{[XCxbnnu NEm4Nm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiwNFk3QCd-MkK4NFA6Pjh:L3G+
A2750  M2Xy[mFxd3C2b4Ppd{BCe3OjeR?= M{T1dlIx|ryP MYGybC=> NHf1cpBFVVOR MWPicI9kc3NiY3HzdIF{\SClbHXheoFo\SCmdYLpcochcGWuZX7hcIlvKHS{ZXH0cYVvfCCjbnSgdoVlfWOnczDheZRweGijZ3njJINmdGxiZHXheIg> NHXPRnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke4OFM3Oyd-MkK3PFQ{PjN:L3G+
U87  M4fsXWFxd3C2b4Ppd{BCe3OjeR?= NIKxc|UzOM7:TR?= MVuyOIg> MXXy[YR2[2W|IITo[UBieG:ydH;zbZMhemG2ZTDpcoR2[2WmIHL5JHBNSUJ? MoToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{i3PFAoRjJ{N{e4O|gxRC:jPh?=
HT1080 MmXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\qT3A2OM7:TR?= MnW5O4Q> M4fRfYlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDpcohq[mm2aX;uJINifXOnZDDifUBkd22kaX7l[EB1emWjdH3lcpQhd2ZiRFPBJIFv\CCRTWC= MnTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEC5PFQoRjJ{N{SwPVg1RC:jPh?=
A549  NUnObIVqSXCxcITvd4l{KEG|c3H5 MoL5OVDPxE1? MYSybC=> MYHwZZJ1cWGubImg[IVkemWjc3XzJJNw\Gm3bTDz[Yxmdmm2ZT3pcoR2[2WmIHHwc5B1d3Orcx?= MmrQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5MkG4NFQoRjJ{N{KxPFA1RC:jPh?=
Primary OPC NULISo5LSXCxcITvd4l{KEG|c3H5 NYK0cXZ7Oc7:TR?= MnXpOogwOjSq M2Do[pJm\HWlZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdHNiaX6g[YFzdHlvIHHu[EBt[XSnLXHwc5B1d3Orcz;u[YNzd3Orcx?= NYjvZW86RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NFc{QDVpPkKyO|A4Ozh3PD;hQi=>
PMNs MWTBdI9xfG:|aYOgRZN{[Xl? MleyOFDPxE1? MnzPOog> NFzt[phFVVOR MYry[ZZmenOnZDD0bIUh[W2xdX70JI9nKGOuZXH2[YQh[2G|cHHz[U0{KHSxIH7lZZIhfmWqaXPs[UBt\X[nbIO= Ml;SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4OUK1O|coRjJ{NkmyOVc4RC:jPh?=
A549  M{nOO2Fxd3C2b4Ppd{BCe3OjeR?= M{G5NFUx|ryP MmXCNYg> NGTQcmJxemW4ZX70d{BieG:ydH;zbZMhcW6mdXPl[EBjgSCEYX;oeY9{cWSnIFm= MoH4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4OEe2N|UoRjJ{Nki3OlM2RC:jPh?=
AGS MXfBdI9xfG:|aYOgRZN{[Xl? Mn[xNlDPxE1? M4rFflEzcA>? MVvpcohq[mm2czD0bIUh[WO2aY\heIlwdiCxZjDwdo8u[2G|cHHz[U0{KGmwIILld5BwdnOnIITvJJRp\SCHdF;BZ{BnemGldHnvci=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ6N{O5PEc,OjJ4OEezPVg9N2F-
shC9  Mlu2RZBweHSxc3nzJGF{e2G7 MoTQNVDPxE1? NGntZ4kyPmh? MVjicJVvfHNiU1jIJIV5eHKnc4Ppc44hcW5ic3jDPUBk\WyuczDlfJBwe2WmIITvJIVqfGincjDQRUBweiCOUFO= NYLtWY9TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFExQTRpPkKyOlQyODl2PD;hQi=>
primary MEFs MoD4SpVv[3Srb36gRZN{[Xl? MUixNog> NYTFeYM1yqCrbnPy[YF{\XNiZYTvdI9{cWSnLXnu[JVk\WRibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBl\XCxbHHybZpifGmxbh?= NFjtWHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[xN|c3Pyd-MkK2NVM4Pjd:L3G+
3T9 MEFs NWXzbZdxTnWwY4Tpc44hSXO|YYm= NF3pb2wyPmh? NVvLdIEycW6lcnXhd4V{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHHmeIVzKGW2b4Dvd4ll\S22cnXheI1mdnR? NVf5Opg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVM4PjdpPkKyOlE{PzZ5PD;hQi=>
3T9 MEFs NFvXPFlHfW6ldHnvckBCe3OjeR?= M{nJT|E5cA>? NGfWOoZ2eHKnZ4XsZZRmeyCrbnn0bYF1d3JiY3HzdIF{\S17IHL1eEBld3ewcnXneYxifGW|IHXm[oVkfG:{IHPhd5Bie2W|IHHmeIVzKGW2b4Dvd4ll\SC2cnXheI1mdnR? NULuWYVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVM4PjdpPkKyOlE{PzZ5PD;hQi=>
MDA-MB-231  M{[0XGFxd3C2b4Ppd{BCe3OjeR?= MYWyNO69VQ>? MU[0[C=> NGr0UVlz\WS3Y3XzJJRp\SClZXzsJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KG2ndH\vdo1qdiC2cnXheI1mdnRid3HzJJNq\26rZnnjZY51dHl? Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OUO0OFEoRjJ{NUmzOFQyRC:jPh?=
C6  M2XC[WFxd3C2b4Ppd{BCe3OjeR?= MVOxNO69VQ>? MUmyOIg> NETwc4VjdG:la4OgeIhmKHO3cIDy[ZN{cX[nIHXm[oVkfCCxZjD0bIUheGWydHnk[UBwdiC4aXHibYxqfHl? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6OEm4NEc,OjJ3OEi5PFA9N2F-
HL-60  NHfCfHpCeG:ydH;zbZMhSXO|YYm= MmrONVAx|ryP NXfOfoZyOjSq NF\De|lqdmirYnn0d{B1cGViY3XscEBieG:ydH;zbZMhe2WuZXP0[YQhMCtrLX3lcpRpgWxizsKtLFHjjpJ4KT3kbYdtfWOxcInyZY5we2mmZdMgOS=> M4XjZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUS2OlY6Lz5{MkW0OlY3QTxxYU6=
HL-60 M2L5[GFxd3C2b4Ppd{BCe3OjeR?= MUCxNE05OM7:TR?= MlX0OIg> NFyxdYVqdmirYnn0d{BVT0iTLXnu[JVk\WRiY3XscEBieG:ydH;zbZPDqA>? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV{M{KyPUc,OjJ3MkOyNlk9N2F-
BCC MXLBdI9xfG:|aYOgRZN{[Xl? MXS1NO69VQ>? M2\COFFp NX\xZWFTcW6qaXLpeJMhTEGWUz3t[YRq[XSnZDDndo94fGhiaX7obYJqfGmxbh?= NEP2dmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxPVQ{Pid-MkK1NVk1OzZ:L3G+
RAW 264.7 MYfBdI9xfG:|aYOgRZN{[Xl? NFvXPWM2OC9zMEFOwG0> M4L0[|Fp MULjc45k\W62cnH0bY9vNWSncHXu[IVvfCCrbnjpZol1eyCGT16tbY5lfWOnZDDyVm5CKGOuZXH2ZYdm Mn[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OUG0NlYoRjJ{NEmxOFI3RC:jPh?=
K562 NUS5UY9TSXCxcITvd4l{KEG|c3H5 M1PGblI2|ryP MXOybC=> M3PyTpBz\X[nboTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGOxLYTy[YF1dWWwdDD3bZRpKGGvdYLlcpNqdiCJIHHu[EBVWkGLTB?= M1\yNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEizO|c4Lz5{MkS4N|c4PzxxYU6=
SGC-7901 NHnBVY5CeG:ydH;zbZMhSXO|YYm= MYeyNEDPxE4EoB?= M4P4elJp MljuZZR1\W63YYTld{BJOk9{wrDvdkBVVkZizsGtbY5lfWOnZDDj[YxtKGGyb4D0c5NqeyCjczD3[YxtKGG|IHPhd5Bie2VvMzDhZ5Rqfmm2edMg NVKzT2Y5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0O|E2QDlpPkKyOFcyPTh7PD;hQi=>
PC3 M{XLVmFxd3C2b4Ppd{BCe3OjeR?= NWrJTGN6OTEQvF2= MX60bC=> M3;wOINwfW62ZYLzJIZt[X[xY3;4bYQucW6mdXPl[EBk[XOyYYPlMZJmdGG2ZXSgZZBweHSxc3nz MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR5MUm3OEc,OjJ2N{G5O|Q9N2F-
SMMC-7721 NXflRZdzSXCxcITvd4l{KEG|c3H5 NWDIRlN7PTEQvF2= MUi0PIg> NUWwdGRb[XS2ZX71ZZRmeyCxVGKtbY5lfWOnZDDhdI9xfG:|aYO= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4NUizN{c,OjJ2NkW4N|M9N2F-
HeLa  MlGyRZBweHSxc3nzJGF{e2G7 MlPkOVDDqM7:TR?= M2q0WFQwQGh? MWDpcohq[mm2czDTWHMucW6mdXPl[EBt[XSnLYDoZZNmKGGyb4D0c5Rq[yCndnXueJM> M2Pqb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[wOVA1Lz5{MkS2NFUxPDxxYU6=
HeLa  NX7DVFhTSXCxcITvd4l{KEG|c3H5 NIm4OHE2OMLizszN M1m4WFFp NHTkO2Z{fXCycnXzd4V{KHSqZTDGVmFRNWmwZIXj[YQh[WOldX31cIF1cW:wIH;mJIFvdmW6aX6gWkBxd3OrdHn2[UBk\Wyucx?= MmT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NEm0OFAoRjJ{NES5OFQxRC:jPh?=
T47D  NVHX[GtDSXCxcITvd4l{KEG|c3H5 M4HEbFExOM7:TR?= Mn;vNYg> NFS2O4RjdG:la4OgeIhmKGenbnXyZZRqd25ib3[gSU1k[WRxQ2TGNkBjgSCVVGO= NX;kWYg3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NFEyPjhpPkKyOFAyOTZ6PD;hQi=>
HeLa  MkjmRZBweHSxc3nzJGF{e2G7 NUDROGdiOzEQvF2= M2jwdFRp MVtCpIlv[3KnYYPld{B1cGViZ3Xu[ZJidCClZXzsJJZq[WKrbHn0fUA1QCCqIHHmeIVzKHCqb4Tv[Jlv[W2rYzD0doVifG2nboVCpC=> NFzDc3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO5OFI1QCd-MkKzPVQzPDh:L3G+
HCC MmjERZBweHSxc3nzJGF{e2G7 NEPtfFYzOCEQvF5CpC=> MX2ybC=> MmrJZZR1\W63YYTld{B1cGViRFjBJIlv\HWlZXSgZYN1cX[jdHnvckBw\iCSQWLQ MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2MkezNkc,OjJ|NEK3N|I9N2F-
mESCs MUfBdI9xfG:|aYOgRZN{[Xl? MVOyMlXPxE1? MXiybC=> NFTrSWhqdmirYnn0d{B1cGViTnHGMY1m\GmjdHXkJINie3Cjc3WgZYN1cX[jdHnvci=> M{\LN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mki1Nlc1Lz5{MkK4OVI4PDxxYU6=
EMT-6  MkLhR5l1d3SxeHnjbZR6KEG|c3H5 NFe5VXMyODEQvF2= NGHTW3kycA>? MVrwZZJ1cWGubImgZoxw[2unZDDj[YxtKGSnYYToJIlv\HWlZXSgZpkhe2m{YX3ld4lv\Q>? NYrwfZp4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOVE6OjFpPkKyNlUyQTJzPD;hQi=>
MCF7 NVTkTY5ySXCxcITvd4l{KEG|c3H5 MVK1NOKh|ryP MVuxbC=> MX3pcohq[mm2czDQRUBi[3SrdnH0[YQh[2G|cHHz[U0{NCBvOTygZY5lKHCxbImoRWRRNXKrYn;z[UkheG:ueX3ldoF{\Q>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ{M{O0OUc,OjJ{MkOzOFU9N2F-
K562 MVjBdI9xfG:|aYOgRZN{[Xl? M2PvXlIxKM7:TdMg M4PGRlQ5cA>? M3;nbmROW09? MlnLZoxw[2u|IHzhdIF1cW6rYj3pcoR2[2WmIHnubIljcXSrb36gc4YhfmmjYnnsbZR6KGGwZDDhdI9xfG:|aYOgbY5lfWO2aX;u MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJzNkG1PEc,OjJ{MU[xOVg9N2F-
Molt4-hyg M{LsUWFxd3C2b4Ppd{BCe3OjeR?= NYnmTmxQOTEQvF2= NVfneppvOC53aB?= NYHnOoN4[myxY3vzJIZiem6nc3;sMYlv\HWlZXSgZ4F{eGG|ZT2zMYxqc2ViYXP0bZZqfHl? NFnpfnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3OVgyOSd-Mk[yO|U5OTF:L3G+
HeLa NYrYbpBrSXCxcITvd4l{KEG|c3H5 M3:3dFEx|ryP MUewMlVp NELOWJlqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSC2aHWgZ49u[mmwZXSgeJJm[XSvZX70JJdqfGhiZ3;tbZNqdiCQIHHu[EBVWkGLTB?= MkfDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzN{m2OlEoRjJ{MUe5OlYyRC:jPh?=
Jurkat T MorqRZBweHSxc3nzJGF{e2G7 M3rNfFMx|ryP M1;EdVAvPWh? MWPEUXNQ NF:2SlhjdG:la4OgeIhmKHqrcnHtMYlv\HWlZXSgZZBweHSxc3nz MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF3OUi5PEc,OjJzNUm4PVg9N2F-
Neutrophil MULBdI9xfG:|aYOgRZN{[Xl? M{XNXlIx|ryP NYrsOlFmOC53aB?= NHKyPWFifHSnboXheIV{KHSqZTDwdo8u[XCxcITveIlkKGWoZnXjeEBw\iCPYWKx NU\je3h6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVY5PDRpPkK2NVk3QDR2PD;hQi=>
HCT116 MVXBdI9xfG:|aYOgRZN{[Xl? MVu1NOKh|ryP M4ny[VJp MUfy[ZZmenOnczDzfY5memerc4TpZ{BieG:ydH;zbZMh\W[oZXP0d{Bw\iClZXzlZ495cWJiYX7kJG5RSy1zNtMg NXfvb3NqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxOVk4PTJpPkKyNVU6PzV{PD;hQi=>
MDA-MB-231  NXnwVpp2SXCxcITvd4l{KEG|c3H5 M3jtRVIvPS15LkZOwG0> MYWybC=> NF75TIlqdmirYnn0d{B1cGViY3XscEBl\WG2aDDv[kBOTEFvTVKtNlMyKGOnbHzzJIlv\HWlZXSgZpkhW0SWIHnuJIEh[2:wY3XueJJifGmxbjDk[ZBmdmSnboSgcYFvdmW{ MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzNUWyOkc,OjJzMUW1NlY9N2F-
LNCaP NGTaT5NCeG:ydH;zbZMhSXO|YYm= NETzb3o1OM7:TR?= NXHZfYZvOmh? NGTOZpdqdmirYnn0d{BjfXSnaX6gbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? NVK0fXlJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNVQ4PjRpPkKyNVE1PzZ2PD;hQi=>
MB231 NXTve4ZXSXCxcITvd4l{KEG|c3H5 MXSxNFDPxE1? M2Gzd|Fp NYmyTIJnTE2VTx?= MVHhZpJw\2G2ZYOgeIhmKGmwZIXjeIlwdiCxZjDj[YxtKGSnYYToJIJ6KFeHRUGgbY5pcWKrdHnvckwhXFKDSVygeJJm[XSvZX70MEBidmRidHjlJINwdWKrbnH0bY9v NF\FcVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGxNlk1OCd-MkKxNVI6PDB:L3G+
HCC38  MojoRZBweHSxc3nzJGF{e2G7 NXHEU3E1OTByzszN MU[xbC=> NIPBVGtFVVOR MnnjZYJzd2ejdHXzJJRp\SCrbnT1Z5Rqd25ib3[gZ4VtdCCmZXH0bEBjgSCZRVWxJIlvcGmkaYTpc44tKFSUQVnMJJRz\WG2bXXueEwh[W6mIITo[UBkd22kaX7heIlwdg>? NX3FSG43RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNVI6PDBpPkKyNVEzQTRyPD;hQi=>
MDA-MB231 MmnHRZBweHSxc3nzJGF{e2G7 NGPOSY02OMLizszN NF\kNo8zPGh? MmfySG1UVw>? NUT5eIhu[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M2jsSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUCxN|M2Lz5{MkGwNVM{PTxxYU6=
LNCaP MX;BdI9xfG:|aYOgRZN{[Xl? M1jwblUxyqEQvF2= Mnn6NlRp NV;4NWZSTE2VTx?= MlPxZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NUS1UXM4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNFE{OzVpPkKyNVAyOzN3PD;hQi=>
HCT116 MoWxRZBweHSxc3nzJGF{e2G7 MYC1NOKh|ryP NETHW2kzPGh? MUXEUXNQ MonhZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NV75PZVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNFE{OzVpPkKyNVAyOzN3PD;hQi=>
Ishikawa  NYrISlJLSXCxcITvd4l{KEG|c3H5 NEPmU3AzPc7:TR?= NHW3T3AzPGh? M4fSeZJm\HWlZYOgZ4VtdCCmZXH0bEBidmRiYYDvdJRwe2m|IHnu[JVk\WRiYomgRoFnKEFz M3u1Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEi4PVE5Lz5{MkC4PFkyQDxxYU6=
 YD-8  NXzGSJhMSXCxcITvd4l{KEG|c3H5 MnnDNVAx|ryP MkLnNYg> NWTvSINVcW6qaXLpeJMhWEyHTz3pcoR2[2WmIHHwc5B1d3Orcx?= MlLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyOE[xPFMoRjJ{MEi2NVg{RC:jPh?=
eosinophil  MYPBdI9xfG:|aYOgRZN{[Xl? MoHhPVDPxE1? NHq5VI8zPGh? NFvCbXBqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSCIYYOgZY51cWKxZIpCpC=> NFz6b4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PVM{PCd-MkKwO|k{OzR:L3G+
L929 M4\mUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWCwSVBJOi53zszN NVfaRnlROjSq MnPQbY5pcWKrdIOgeIhmKGW6cILld5Nqd25ib3[gdE1xOzhiYX7kJG5HNc78QjD0c{BifWevZX70JHRPTs7zLXnu[JVk\WRibnXjdo9xfG:|aYOgZY5lKGG3dH;wbIFogQ>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{N{C5O{c,OjJyMkewPVc9N2F-
YD-8  M2r4N2Fxd3C2b4Ppd{BCe3OjeR?= NXe5ZlRVOTByzszN MXmxbC=> MUDicI9kc3NidHjlJGdUNUiFbD3pcoR2[2WmIHHwc5B1d3Orcx?= M3m4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKwNFc5Lz5{MkCyNFA4QDxxYU6=
HBx MnXNRZBweHSxc3nzJGF{e2G7 MnPxNlXPxE1? MVG0PIg> MknCSG1UVw>? M1niSpJm\HWlZXSgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDNvTVG= MkHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkCwO|goRjJ{MEKwNFc5RC:jPh?=
U937  Mlf0RZBweHSxc3nzJGF{e2G7 Mk\iOVDDqM7:TR?= NHXrPVYycA>? MYrpcohq[mm2czDISk1qdmS3Y3XkJIFxd3C2b4Ppd:Kh M3XXbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUm4O|MyLz5{MUm5PFc{OTxxYU6=
HL60 M3vLcmFxd3C2b4Ppd{BCe3OjeR?= M1rubFQx|ryP NFjnPGo1OG2rbh?= MYXEUXNQ NWPLWmVb[myxY3vzJGJPTENiY3;tdI92dmS|IHnu[JVk\SCneIDvd5Vz\SCxZjDwbI9{eGijdHnkfYx{\XKrbnWgZY5lKESQQTDmdoFodWWwdHH0bY9v MoPSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OEOyPVYoRjJzOUizNlk3RC:jPh?=
M-14 NWXDWHRLSXCxcITvd4l{KEG|c3H5 MViyOe69VQ>? NFXr[o0xNjWq MYpCpIlvcGmkaYTzJIJwfGhidHjlJINzfWSnIHX4eJJi[3RvIHHu[EBkd22yb4Xu[E1qdmS3Y3XkJIFxd3C2b4Ppdy=> M3rn[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUW0PVU6Lz5{MUm1OFk2QTxxYU6=
SK-BR-3 M3XJVGFxd3C2b4Ppd{BCe3OjeR?= NGDj[mw2OMLizszN M320XVJp NU\aN2xq[myxY3vzJIFxd3C2b4TpZ{BFVkFiZoLh[41mdnSjdHnvckBqdmS3Y3XkJIJ6KGOxbXLpcoF1cW:wIH;mJHNONTF4NDDhcoQhemGmaXH0bY9v NVvvfWVmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5NFE{QDZpPkKxPVAyOzh4PD;hQi=>
MDA-MB-468 Mn7sRZBweHSxc3nzJGF{e2G7 MUK1NOKh|ryP M3HmcVJp MmDMZoxw[2u|IHHwc5B1d3SrYzDEUmEh\nKjZ33lcpRifGmxbjDpcoR2[2WmIHL5JINwdWKrbnH0bY9vKG:oIGPNMVE3PCCjbnSgdoFlcWG2aX;u NGHa[GU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwNVM5Pid-MkG5NFE{QDZ:L3G+
Assay
Methods Test Index PMID
Western blot IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3 ; Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II ; p23 / Hsp90 / hTRET / MMP2 ; cyto C 27191889 19740745 17301139
Growth inhibition assay Cell viability 12522090
Immunofluorescence cytochrome c ; Smac/DIABLO ; Endo G ; AIF 11726499 12941691
ELISA IL-1β 26254306
In vivo

In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo[7].

Protocol (from reference)

Cell Research:

[6]

  • Cell lines: human granulosa cell lines (GC1a, HGL5, COV434)
  • Concentrations: 50 μM
  • Incubation Time: 48 h
  • Method:

    To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h with etoposide (50 μg/ml) and/or Z-VAD-FMK (50 μM) under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses.

Animal Research:

[7]

  • Animal Models: CD1 mice
  • Dosages: 10 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+35 %PEG 300+2%Tween 80+ddH2O
For best results, use promptly after mixing.

6mg/mL

Chemical Information

Molecular Weight 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Can you suggest an in vivo formulation of S7023 for animal studies?

Answer:
For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

Question 2:
What is difference between S7023 & S8102?

Answer:
Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

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