Z-VAD-FMK

Catalog No.S7023

Z-VAD-FMK Chemical Structure

Molecular Weight(MW): 467.49

Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

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Cited by 90 Publications

Purity & Quality Control

Choose Selective Caspase Inhibitors

Biological Activity

Description Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Features A key compound for apoptosis studies.
Targets
Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro

Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
GC1a MVfBdI9xfG:|aYOgRZN{[Xl? MVy1NO69VQ>? NVzDXYdVOjSq NIrYN2dqdmSrY3H0[ZMh[SCycn;0[YN1cX[nIHXm[oVkfCCjZ3HpcpN1KGW2b4Dvd4ll\Q>? NYH1eFlJOjZzNkmwO|U>
HGL5 NFvXOZFCeG:ydH;zbZMhSXO|YYm= M1vDdlUx|ryP MkWxNlRp MV3pcoRq[2G2ZYOgZUBxem:2ZXP0bZZmKGWoZnXjeEBi\2GrboP0JIV1d3Cxc3nk[S=> MXmyOlE3QTB5NR?=
HepG2 Mmm4RZBweHSxc3nzJGF{e2G7 NXvBdHdXOjEQvF2= MkjWNYg> MUnheJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMg NGfad4UzPjF4NEe5OS=>
BEL-7402 MVrBdI9xfG:|aYOgRZN{[Xl? MkDjNlDPxE1? NX75U2VtOWh? Ml22ZZR1\W63YYTl[EB1cGViYYDvdJRwfGmlIHnu[JVkfGmxbjDv[kBKUUlvMUFCpC=> M2TCPVI3OTZ2N{m1
CEF NIi0dGhMcW6jc3WgRZN{[Xl? M3nweFAtOzNuNkesNVAx|ryP M2eze|E2dWmw MYjkc5dvNXKnZ4XsZZRmeyCSUjDlcpp6dWViYXP0bZZqfHlidH:gOFDwxIViYYSgNVAx|ryP NFW5VpAzPjFyMkOzPS=>
SP2/0 MoPoRZBweHSxc3nzJGF{e2G7 NUK1fldkOTByzszN NVG1VpVROWh? NYK3Z3R1TE2VTx?= NFz1[mpjdG:la4OgeIhmKGGyb4D0c5NqeyCxZjDTVFIwOCClZXzsdy=> NVThW41tOjZyN{S3N|I>
HUVEC-2c MofGRZBweHSxc3nzJGF{e2G7 NYnpZYZWPTEQvF2= NYnO[W54Pmh? M3jpPYRm[3KnYYPl[EB1cGVib4itUGRNNWmwZIXj[YQh[XW2b4DoZYd6 M1XFd|I3ODJzN{K5
U1 NYDmVZdVSXCxcITvd4l{KEG|c3H5 MXqwMVExOM7:TR?= NIXwT4YzcA>? NYjtbpZ3emWmdXPld{BlenWpLXnu[JVk\WRiYYDvdJRwe2m|IHHu[EB{fWK|ZYH1[Y51KEiLVj2xJJJmeGyrY3H0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M2L6XVI2QThyOUSy
ACH-2 M3myVmFxd3C2b4Ppd{BCe3OjeR?= MUmwMVIxOM7:TR?= NWTmT5lNOmh? NFPLZmVz\WS3Y3XzJIRzfWdvaX7keYNm\CCjcH;weI9{cXNiYX7kJJN2[nOncYXlcpQhUEmYLUGgdoVxdGmlYYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MUiyOVk5ODl2Mh?=
U1 NYr4TpJlU2mwYYPlJGF{e2G7 MYixNFDPxE1? MnPRNog> M3rXdYlvcGmkaYTzJINie3Cjc3WtNy=> NV7BVnFROjV7OEC5OFI>
A549/V16 NEDjVFNCeG:ydH;zbZMhSXO|YYm= MVm1NO69VQ>? NF3jNIEycA>? M{\1cpJmeHKnc4Pld{BVTy:WTT3pcoR2[2WmIHHwc5B1d3Orcx?= NVv4R4p4OjV7NE[wN|M>
SGN NVnNdZpWSXCxcITvd4l{KEG|c3H5 Ml;HNlBuVQ>? MUi0PIg> NX;6XGRscGG|IH7vJIlv\my3ZX7j[UBwdiCDSV[sJINidHCjaX6g[ZhxemW|c3nvckBweiClZXzsJIFxd3C2b4Ppdy=> NETER3UzPTh5NE[zNy=>
HCT116  NXXEe3F{SXCxcITvd4l{KEG|c3H5 M4rYPVUx|ryP M4TF[FJp NUnMS|JbcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigbY5lfWOnZDDifUBFTF[S NWKzdIJNOjV6Nki4NVg>
DTK-SME NUjNRXE1SXCxcITvd4l{KEG|c3H5 MVi1NO69VQ>? MkPaNog> NGr2UXZFVVOR M4D5PZBienSrYXzsfUBqdmirYnn0d{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgZpVxcX[jY3HpcoU> M4S4NlI2QDR|OEm3
HL-60 NWDnT5B4SXCxcITvd4l{KEG|c3H5 MmTvOVDPxE1? NULNbWxpPDiq NFjsSWNz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KFONST3JTUB1emWjdH3lcpTDqA>? MkP2NlU5OjRyNEO=
U937 NHP0W5ZCeG:ydH;zbZMhSXO|YYm= NXmzW|l3PTEQvF2= MmfsOFhp NEXuUFlz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KFONST3JTUB1emWjdH3lcpTDqA>? MlTwNlU5OjRyNEO=
A549 M1jPTGFxd3C2b4Ppd{BCe3OjeR?= M2r6TVIxKM7:TR?= NUHL[pVuOjSq MYXy[YR2[2W|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JGhHS1BidILlZZRu\W62 NUPhPWVvOjV5OUSxOFk>
HeLa MoPyRZBweHSxc3nzJGF{e2G7 NFvLZXAyOM7:TR?= NVLRR5NMPDiq NUfiOWd3\GWlbHnu[ZMhfGinIILheIUhd2ZiYYDvdJRwe2m|IHTyZY1ifGmlYXzsfS=> M2rM[lI2Pzd{NUS1
SGC-7901 NGPpPJFCeG:ydH;zbZMhSXO|YYm= MlvNNlDDqM7:TR?= Mn7NNYg> MnT2bY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDvfIFtcXCuYYTpci=> NH31enEzPTd4N{C3Oi=>
MCF-7 MXzBdI9xfG:|aYOgRZN{[Xl? NYLYb3RYOTEQvF2= NHniUo0zcA>? NHG0fFRqdmirYnn0d{B1cGViY3XscEBl\WG2aDDpcoR2[2WmIHL5JFND MonlNlU4OjJzMUS=
A549 Mn\ERZBweHSxc3nzJGF{e2G7 MnfyNVAh|ryP MYCyOIg> NEW0SlhFVVOR MVvwdo9ud3SnczDIRmMufHKnYYTl[EBCPTR7IHPlcIwhe3W{dnn2ZYwhLiCjdITlcpVifGW|IITo[UBkdGWjdnXkJHBCWlBiZYjwdoV{e2mxbh?= MlizNlU3QDN3Nki=
U87 NYLxOZdYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX21NO69VQ>? NWnENZgzOWh? NHXkPJNz\WOxdnXyd{Bk\WyuIHfyc5d1cCCocn;tJHROYiC2cnXheI1mdnR? NHzSXGszPTZ6MU[2PC=>
Neocortical Neuron M2jlTG5mfXKxdH;4bYNqfHliQYPzZZk> MYKxNFDPxE1? MUOxbC=> MVPhcpRi\2:waYrld{BJd2mjbXnk[UBCNUmwZIXj[YQhVmW3cn;0c5hq[2m2eR?= NHP0d3QzPTZ5NUCwNS=>
Primary human placental cytotrophoblasts MkLERZBweHSxc3nzJGF{e2G7 NW[xcGRSOzEQvF2= NXHu[pVsOjSq MVPEUXNQ NEDRbopz\X[ncoPld{B1cGViaX7obYJqfGmxbjDv[kAyOc7{LVjTSFIh[nlidILpZ4xwe2GwwrC= NXfOXHlzOjV4NEK1PVI>
MM MV3BdI9xfG:|aYOgRZN{[Xl? M3jpWFUx|ryP NF;pS3EzOG2rbh?= MkfBdIFzfGy7IHnubIljcXS|IGPIT{1qdmS3Y3XkJEBk\WyuIHTlZZRp MoXENlU2OzByOUi=
DLD1 MVTBdI9xfG:|aYOgRZN{[Xl? M1HMSVIxyqEQvF2= NXO3cldGOWh? NVvvRZQ3eGG{dHz5JJJmfmW{c3XzJINmdGxiYYDvdJRwe2m|IHPheZNm\CCkeTDXV3Ay M3myWVI2PTJ2MkS2
MDA-MB-231 NEXYN3FEgXSxdH;4bYNqfHliQYPzZZk> M3jlTFEx|ryP Mnn0NYg> M4fhSYF2\22nboTzJINmdGxiZHXheIgh[W[2ZYKgR2hWSSC2cnXheI1mdnR? NWPrfHRJOjV3MkG1NFE>
MKN28 MVjBdI9xfG:|aYOgRZN{[Xl? MnPwNVDPxE1? MW[zNI1qdg>? NFu5OlVFVVOR NYXacoIycW6qaXLpeJMhXE6ILd8xJJBtfXNiQ1jYMYlv\HWlZXSgZZBweHSxc3nz NYToSVdxOjV3MUO5OlA>
HL-60 NUnhbZdFS3m2b4TvfIlkcXS7IFHzd4F6 NF7TNGQyODEQvF2= NGPDO5QycA>? MUfEUXNQ M4P4TOKhcW2ycn;2[ZMhfmmjYnnsbZR6KG:oIGTDUmF{NXS{ZXH0[YQh[2WubIO= NHvoeWIzPTVyMkmzNi=>
AGS MUXBdI9xfG:|aYOgRZN{[Xl? NWPxNnNGOTEQvF2= MW[xbC=> MUfwdoV3\W62czDjeZJkfW2rbj3pcoR2[2WmIHPs[YF3[WenIH;mJINie3Cjc3WtN{whNThuIHHu[EAuQSCycn;0[Ylvew>? NWjye|lIOjV2OUKyNVQ>
A549  MnO0RZBweHSxc3nzJGF{e2G7 M3\VUVEx|ryP NFvoOFMzPGh? NGXMfJhjdG:la4OgSG1CWy2rbnT1Z4VlKGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzDhcoQhWEGUUDDhcoQh[XCxcITveIlkKGOnbHyg[IVifGh? NGXhWpQzPTR|NEm4PS=>
INS-1 NEDXV3BCeG:ydH;zbZMhSXO|YYm= MXm1NO69VQ>? MXW2bC=> M2HReWROW09? NETFd|dl\WO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh\WG{bImgZZBweHSxdHnjJINmdGy|wrC= MnLLNlU1OzB6OUe=
Primary hepatocytes  NVvYWJBrSXCxcITvd4l{KEG|c3H5 Mm[yOVDPxE1? NVPjZmRPOTiq MWHpcohq[mm2czDhdI9xfG:|aYOgc4YhcGWyYYTvZ5l1\XNiaX7keYNm\CCkeTDBZ5QhTCCjbnSgWG5HNc7z MlvzNlU1ODd3M{i=
L929-A  NYS3cW4yS3m2b4TvfIlkcXS7IFHzd4F6 MV2yNOKh|ryP NInPRYEzPGh? Mo\FbY5pcWKrdIOgWG5H|rFvaX7keYNm\CClZXzsJIRm[XSq NHm2NoczPTN7OEW0NC=>
L929-N NGPjXo9EgXSxdH;4bYNqfHliQYPzZZk> M1rmNVIxyqEQvF2= MoGxNlRp NHvESWhmdmijbnPld{Bl\WG2aDD2bYEh[XW2b3PybY5mKFSQRt8xJJBzd2S3Y4Tpc44> Mk\UNlU{QTh3NEC=
Cytotoxicity Assay NV;j[lBUS2WubDDWbYFjcWyrdImgRZN{[Xl? MVWyNO69VQ>? MoDjOFhp MXnwdoV3\W62IF3IUWQucW6mdXPl[EBk\WyuIHTlZZRp MUWyOVM6OjFzNh?=
COS7 NGnqdpFMcW6jc3WgRZN{[Xl? NXGxdYlQOTEQvF2= NXXQfYtqOjSq Mn;lbY5kemWjc3XzJINie3Cjc3WgN{84KGGldHn2bZRq\XN? NW\tOJhJOjV|OESwNlU>
COS7 Mn\NRZBweHSxc3nzJGF{e2G7 MUixNO69VQ>? NYe3[WRFPDiq MknxdIFzfGmjbHz5JJBz\X[nboTl[EBHSzFyMT3pcoR2[2WmIHPlcIwh\GWjdHi= MX2yOVM5PDB{NR?=
A375 M3\vOWFxd3C2b4Ppd{BCe3OjeR?= NHXSPHM{OM7:TR?= MoPqNog> MVXwdoV3\W62czD0bIUh\HK3Zz3pcoR2[2WmIGDBVnAh[2ynYY\h[4U> MUmyOVM4PjFzNR?=
A549 MX3BdI9xfG:|aYOgRZN{[Xl? MYi1NO69VQ>? M3f6SFI1cA>? M3vkO5JmfmW{c3XzJJJq[m:|b33lJIJqd2enbnXzbZMh[W6mIHHwc5B1d3OrczDjZZV{\WRiYomgJGNpcWqxbnfkZY4> NV7FcplGOjV|NEm3PFE>
A549 M1fwcGFxd3C2b4Ppd{BCe3OjeR?= NWSwW|FUOi53LUK1{txO NWTS[2Q1OWh? NG\zXo9l\WO{ZXHz[ZMhfGinIIDvdJVt[XSrb36gc4Yh[XCxcITveIlkKGOnbHzzJIRmeGWwZDDvckBkd26lZX70doF1cW:wcx?= MXOyOVM1OjR{Nx?=
 NBL-W-S  MnK4RZBweHSxc3nzJGF{e2G7 MWG1NO69VQ>? NVXtWXpQOWh? MVzmeYxtgSC{ZYPjeYV{KGOnbHygeoli[mmuaYT5JIFnfGW{IFfBUnQuPjFidILlZZRu\W62 Ml;iNlU{OjN{MkK=
Caki-1  NYPxWll6SXCxcITvd4l{KEG|c3H5 NH;vUVg1OM7:TR?= M{S0OVQ5cA>? M1Tyc5Jm\HWlZYOgeIhmKG63bXLldkBw\iCDbn7lfIlvKFZvcH;zbZRqfmViY3XscJM> MWWyOVI4QTF7MR?=
769-P NFv1bVZCeG:ydH;zbZMhSXO|YYm= NFuxT5Q1OM7:TR?= M2TYUVQ5cA>? MULy[YR2[2W|IITo[UBvfW2kZYKgc4YhSW6wZYjpckBXNXCxc3n0bZZmKGOnbHzz M2riWVI2Ojd7MUmx
H9c2 Mk\URZBweHSxc3nzJGF{e2G7 Mn24OVDPxE1? MVmxbC=> M{\KU4lvcGmkaYTzJGRQYC2rbnT1Z4VlKGOjc4Dhd4UhOyCjY4TpeoF1cW:wIHL1eEBvd3RidHjlJIxwe3Nib3[gZ4VtdHN? M4fScFI2Ojh|OEG5
K562 Ml;jRZBweHSxc3nzJGF{e2G7 MYW1NO69VQ>? M{LTclRp NUfLSHVbcW6qaXLpeJMhUmGlLVGtbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? MWiyOVI1OTZzOR?=
L929 NV;BfZNiSXCxcITvd4l{KEG|c3H5 MVyxNO69VQ>? M2j4dlZp M3\RbYlv\HWlZYOgcoVkem:ydH;zbZMhf2m2aDDUUmY> M1vsNFI2OTl3Nk[w
A549 M17ZUmFxd3C2b4Ppd{BCe3OjeR?= MXWxNO69VQ>? NUL6UFFmO2h? NHi2UIJz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHPheZNm\CCkeTDQVXE> NXPYV3p4OjVzNkG2PVk>
MCF-7 MWXBdI9xfG:|aYOgRZN{[Xl? NE\PeGYzOM7:TR?= NXnmV|hoOmh? M3LROIRm[3KnYYPld{BMTFJvc3nSUmEucW6mdXPl[EBieG:ydH;zbZM> M2PJOlI2OTh{MkSw
OS NGf4[29CeG:ydH;zbZMhSXO|YYm= NIjNcpUzOC92MN88US=> MmDSO|Jp M{jWOYlvcGmkaYTzJG9UKGOnbHygeoli[mmuaYT5JJJm\HWldHnvckBjgSCFNjDj[ZJidWmmZR?= MknJNlUyPTJ|OUm=
HUVECs MVHBdI9xfG:|aYOgRZN{[Xl? M1mwO|Ex|ryP M2fUSlJp MYZCpIlvcGmkaYTzJIFxd3C2b4Ppd{BidmRiZnHjbYxqfGG2ZYOgZZV1d3CqYXf5JIlvKESHTm[yMYlv\mWldHXkJGhWXkWFcx?= MWOyOVE{QDdyMx?=
EA.hy926 M2X5Z2Fxd3C2b4Ppd{BCe3OjeR?= M2XTRVEx|ryP NWPrcG52Omh? NWrO[2FmyqCrbnjpZol1eyCjcH;weI9{cXNiYX7kJIZi[2muaYTheIV{KGG3dH;wbIFogSCrbjDESW5XOi2rbn\lZ5Rm\CCHQT7ofVkzPg>? M2fIU|I2OTN6N{Cz
Ebs MXXBdI9xfG:|aYOgRZN{[Xl? MVWxNE0yODEQvF2= NV7IWXRbOjSq MmnoxsBOVVSVIHflcoVz[XSrb36gdoF1\SCmZXPy[YF{\WRiYYOgeIhmKGOxbnPlcpRz[XSrb36gc4Yhgi2YQVSu[o1sKGmwY4LlZZNm\A>? M3iyO|I2OTN2OEG3
Jurkat NUL4bmJ7SXCxcITvd4l{KEG|c3H5 MmjSNlDPxE1? NYD1N4pZOjSq M3fxb2ROW09? NXfPV3JLeGG{dHnhcIx6KGmwaHnibZQh[2WubDDk[YF1cCCxZjDKeZJs[XRiY3XscJMhcW6mdXPl[EBjgSBzMEC1PE1HPCClb33ibY5m\CC5aYToJHZRScLi NFXtd5ozPTF{MEeyNy=>
K562 MmL2RZBweHSxc3nzJGF{e2G7 M1n2UlAvOS1zzszN MlHMNYg> MXLpcohq[mm2czDjcIVifmGpZTDv[kBJW1B7MDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MUKyOVEyQTF6OB?=
IMR-32 NVjrO3JxSXCxcITvd4l{KEG|c3H5 MVu0NOKh|ryPwrC= NXP2[mhsOmh? M3PBOYRm[3KnYYPlJIlvKGGyb4D0c5Rq[yClZXzsd{Bkd22yYYLl[EB1dyCWLUKgeI95cW5? Mn7nNlUxQDR5NUW=
RPMI 8226 M{T4TmFxd3C2b4Ppd{BCe3OjeR?= MnexNVAx|ryP NFnCfJQycA>? MUHhcI1we3RiY3;tdIxmfGWueTDicI9kc3NiY3XscEBl\WG2aDDjZZV{\WRiYomgcoVkem:|dHH0bY4uOQ>? NYO2S2oxOjVyM{e4OVE>
U266  MUTBdI9xfG:|aYOgRZN{[Xl? NIe2WI8yODEQvF2= MWmxbC=> NGjLRZFx[XK2aXHscJkh[myxY3vzJINmdGxiZHXheIgh[2G3c3XkJIJ6KG6nY4Lvd5RifGmwLUG= MV6yOVA{Pzh3MR?=
H929  MUDBdI9xfG:|aYOgRZN{[Xl? M3PxXlExOM7:TR?= M1HzW|Fp NELFdXVx[XK2aXHscJkh[myxY3vzJINmdGxiZHXheIgh[2G3c3XkJIJ6KG6nY4Lvd5RifGmwLUG= NGDiR4wzPTB|N{i1NS=>
MM.1S  NFTIUYlCeG:ydH;zbZMhSXO|YYm= Mki2NVAx|ryP MmjjNYg> Mnvy[YZncWOrZX70cJkheHKndnXueJMhe2:{YX\lcoljNWmwZIXj[YQh[2WubDDk[YF1cCClb33ibY5mKHerdHigcoVkem:|dHH0bY4uOQ>? MXSyOVA{Pzh3MR?=
C6 M2LDV2Fxd3C2b4Ppd{BCe3OjeR?= NYfkc2tnPTEQvF2= MYW0PIg> NIj6OodxemW4ZX70d{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBk[XW|ZXSgZpkheHKnZ37lco9td26n MknoNlUxOTN2N{m=
AGS NFn3WIJCeG:ydH;zbZMhSXO|YYm= NV3kWlBDPTEQvF2= MoHJNlRp MkXJZYJwdGm|aIOg{tIudGGyYXPoc45mNWmwZIXj[YQh[2WubDDk[YF1cCCjbnSgbY5pcWKrdHXkJIdzd3e2aB?= MkjINlUxODl4OUi=
HeLa  NWrGUXVFSXCxcITvd4l{KEG|c3H5 NEjRRWQ1OM7:TR?= MWmyOIg> NXuyOYY5cW6qaXLpeJMhfGinIHnuZ5Jm[XOnZDDhdI9xfG:|aYOgbY5lfWOnZDDifUB{cVKQRkGyNS=> MkXVNlQ6Ojh4OEW=
THP-1  Mmn2RZBweHSxc3nzJGF{e2G7 MWGxNO69VQ>? MXyxbC=> MnXkdoVlfWOnczDhdI9xfG:|aYOgbY5lfWOnZDDifUBCVEFvU1TUxsA> NIrPVXgzPDl{M{[1Ny=>
P815  MYrBdI9xfG:|aYOgRZN{[Xl? MWKxNFAhyrWP NX;NbZpUOTKq NH3USYRFVVOR MoDHbY5pcWKrdIOgeolzfXNvaX7keYNm\CCjcH;weI9{cXN? MXKyOFkzOzJ5Mx?=
HCT116 M1P1bWFxd3C2b4Ppd{BCe3OjeR?= NWrNV|BrOjEQvF2= MUiyOIg> MofHZYJzd2ejdHXzJI9nKFSTLXnu[JVk\WRiYYDvdJRwe2l? NVj2V4VGOjR6OUC0OFk>
SW1116  MW\BdI9xfG:|aYOgRZN{[Xl? M{LSZVExKML3TR?= NVzVRmZIOWh? MoXiSG1UVw>? NYTY[nFWemW|dILhbY5{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkh\2WoaYTpcoljKHCudYOg[IVkcXSjYnnu[S=> NICzbZczPDh5NEK4Oi=>
LOVO NESyb3VCeG:ydH;zbZMhSXO|YYm= MUWxNEDDvU1? MknTNYg> NHrUW5hFVVOR MULy[ZN1emGrboOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDn[YZqfGmwaXKgdIx2eyCmZXPpeIFjcW6n MofPNlQ5PzR{OE[=
SNU449  NX[1bI5ESXCxcITvd4l{KEG|c3H5 NFTTfXgzOM7:TR?= NWW1e45KPDiq MWXEUXNQ M3\jS4Rm[3KnYYPld{BucVJvNEWxMYlv\HWlZXSgZZBweHSxdHnj MlK1NlQ5PDF4M{i=
Caco-2 NF;4Z4ZCeG:ydH;zbZMhSXO|YYm= NXm1c5ZUPDEkgJpOwG0> M4O5T|Rp MnvkdJJmfmWwdIOgV3QuPy2rbnT1Z4VlKFqRLUGgZ4hidmenczDhcoQhXEWUIHTyc5A> MnrtNlQ5OjJzOEO=
macrophage NFTHbJVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1L4bVAuOjByzszN NYHQOJFJOjSq MnP2bY5lfWOnczDUUmYuKGGwZDDSbZA{NWSncHXu[IVvfCCwZXPyc5B1d3OrczDpckBu[WO{b4DoZYdm NX;TcJBFOjR5OUm1OlU>
AGS  NV;1bmVVSXCxcITvd4l{KEG|c3H5 NITrPI4zOM7:TR?= Mn3ONVJp MWfEUXNQ MXHy[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJJJme3CxboPlJJRwKHSqZTDFeG9C[yCocnHjeIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MnXUNlQ4QDl5MEO=
LCC9  NFq3T5RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MoHaNVAx|ryP MoXQOYQ> Mor6Zoxw[2u|IHPlcIwh\GWjdHigbY5lfWOnZDDifUBkd22kaX7heIlwdiCOMUegZY5lKGOqbH;yc5F2cW6n NWrY[HN3OjR5OEWyOVY>
U937  M{HM[I5m[3KxcITvd4l{KEG|c3H5 NUjEVZkxOTEEoN88US=> MXOzNOKhdWmw NUHvcI16cW6mdXPld{Bv\WO{b4D0c5NqeyClb33ibY5mKHerdHigWG5H M1HIZVI1Pzd|N{W2
T cell M1TNemNmdGxicILvcIln\XKjdHnvckBie3OjeR?= NFf4d|YxNTFyMN88US=> M2LOdFczcA>? NFHpcFJKSzVyPUewJO69VSxiaX7obYJqfHNiYX70bU1ETDNvaX7keYNm\CCWIHPlcIwheHKxbHnm[ZJifGmxbjDpckBRSk2Fcx?= MYeyOFc3QDdyNx?=
HeLa  MmTBRZBweHSxc3nzJGF{e2G7 NHLsN442OM7:TR?= M1j6RlczcA>? NVXqTJA1TE2VTx?= MV\wdoV3\W62czDhdI9xfG:|aYOgbY5lfWOnZDDifUBkd22yb4Xu[JPDqDIkgKO1 MYCyOFc2PDd6Nh?=
HeLa  NIf3S3FCeG:ydH;zbZMhSXO|YYm= NIHVd3YyODEQvF2= NFH2XmkzPGh? NInJ[Ix{fXCycnXzd4V{KHKjdHWgc4Yh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IH;2[ZJmgHC{ZYPzbY9vKG:oIFe1PXMhd3JiR{exVkBxOTVyZ3z1[YTDqA>? MVOyOFczOjR4OB?=
IEC-6  M1;DV2Fxd3C2b4Ppd{BCe3OjeR?= NYjHcW1QOTEQvF2= MViyOIg> M1H4VpBz\X[nboTzJHRk\EFvaX7keYNm\CClYYPwZZNmNTNiY3zlZZZi\2ViYX7kJO6zNWOjdHXubY4h\GWpcnHkZZRqd25? Ml\FNlQ4OTF3N{G=
HCT116 MmjnRZBweHSxc3nzJGF{e2G7 M{mwOFIx|ryP MmG4NlRp MV;pcohq[mm2czD0bIUh[2WubDDhdI9xfG:2aXOgbY5lfWOnZDDifUB[VFR{MEW= NF;QSJMzPDdzM{ixNi=>
L02 MmLYRZBweHSxc3nzJGF{e2G7 NFm5b4wzOM7:TR?= NGT1O3QycA>? M17qV5Bz\X[nboTzJJRp\SClZXzsJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KE2HSGC= NW\0NGFOOjR5ME[0OlE>
A375 MWjBdI9xfG:|aYOgRZN{[Xl? NHzSe5gzOM7:TR?= MnW3NlRp NXfX[41WemW4ZYLz[ZMh\GmlaYTybY5wdmViQj3pcoR2[2WmIHHwc5B1d3Orcx?= MWmyOFY6QTFzMR?=
HDPC MnTMRZBweHSxc3nzJGF{e2G7 MmLSOVDDvU1? MojnNlRp NIPSSXVqdmirYnn0d{BPVy2rbnT1Z4VlKGGyb4D0c5Nqew>? MkS2NlQ3OzR3OUO=
A549 NWnhd|dKSXCxcITvd4l{KEG|c3H5 MUi1NOK2VQ>? MXeybC=> MmW1SG1UVw>? Ml\LdJJmfmWwdIOgeIhmKGi7cH;kbZBtd2mmIFTORUBkd262ZX70JJBp[XOnIHnu[JVk\WRiYomgZ4VxcGGub3Podo9ucW5? M4n6dlI1PTh6MUO1
 BV-2 Mm\YRZBweHSxc3nzJGF{e2G7 NU\tSYY{OjEQvF2= MX[ybC=> M13nPJN2eHC{ZYPz[ZMhXVZvaX7keYNm\CClaILvcYF1cW5iZYj0dpV{cW:wIHHu[EBlcWyjdHnvckBw\iC2aHWgcpVkdGWjcjDlcpZmdG:yZR?= NIXlXXQzPDV3OEGxPC=>
HT-29  MlTCRZBweHSxc3nzJGF{e2G7 M4TJbFUx|ryP NVexc2dKPDiq MXTicI9kc3NidHjlJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGujZX3w[oVzd2x? MX2yOFU1QTF5NR?=
A549 MkLHRZBweHSxc3nzJGF{e2G7 NIDRWVk2|ryP NEO1WIgzPGh? MYDzeZBxemW|c3XzJFZmyqBqQluxNFA1OClvaX7keYNm\CCjcH;weI9{cXQEoB?= MkTXNlQ2Ojl6N{G=
MIA-PaCa-2 MlLERZBweHSxc3nzJGF{e2G7 M2\UZlI1cA>? NG\lXWVjdG:la4OgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[U0{KGmwZIXj[YQh[nliYn;0bEBCNTR2M{[1OEBidmRicHHjcIl1[XinbB?= NXT6OWUxOjR3MUC5PVI>
Caki-1 NVHNfGhtSXCxcITvd4l{KEG|c3H5 NWXwUlhtOjEQvF2= MVmxbC=> M1\QOYlvcGmkaYTzJHNEWC2rbnT1Z4VlKGGyb4D0c5Nqew>? MWSyOFUxPDZ6MR?=
CLL MlHTRZBweHSxc3nzJGF{e2G7 M{\WUFI2|ryP NVvZTGxuOWh? NH6w[25x[XK2aXHscJkh[myxY3vzJG1NVjJ{M{itbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? MWqyOFQ3PzZ|NB?=
SGC-7901 M2LSdWFxd3C2b4Ppd{BCe3OjeR?= MVixNO69VQ>? M4jN[VI1cA>? M4LkNZBzd22xdHXzJJRp\SCFR1nJMYlvcGmkaYTl[EBk\WyuIHfyc5d1cCCrbjDTS2MuPzlyMTDj[Yxtew>? MUmyOFQ2PDR6OB?=
k1735 NVj5empnSXCxcITvd4l{KEG|c3H5 Mn\VNlDjiIoQvH2= NVK1NnNwPGh? M16wVolvcGmkaYTzxsBU[Wyvb37lcIxiNWmwZIXj[YQh[XCxcITvd4l{ M4PjU|I1PDVzMUG2
HaCaT  M17XZWFxd3C2b4Ppd{BCe3OjeR?= NHn6bGMyODEQvF2= NF\I[mMycA>? MknuZox2dnS|IGXWRk1qdmS3Y3XkJIFxd3C2b4Ppd{BqdiCKYVPhWEBk\Wyucx?= MVSyOFM2Pjl7Nx?=
podocytes M1;sSmFxd3C2b4Ppd{BCe3OjeR?= M1vUbFIxOM7:TR?= M3q4PFZp MmnpbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhOyx2LVTHSS=> M1e4RlI1OzN5N{e3
 KB NXy0OFFTSXCxcITvd4l{KEG|c3H5 M4LTSlUx|ryP NFzuemIzPGh? MX3pcohq[mm2czDMbYNwNUFvaX7keYNm\CClYYPwZZNmNTNiYX7kJHBCWlBiYXP0bZZifGmxbh?= M4nmXVI1OzN5NEmy
 HepG2  M4r6UmFxd3C2b4Ppd{BCe3OjeR?= M{TVd|Ex|ryP M2n1dVI1cA>? NEDUSHpz\WS3Y3XzJJZqdWWwdHnuJINt\WG4YXflJINifXOnZDDifUBNWFN? NFvIUI4zPDN{NUixOi=>
fetal rat lung fibroblasts  M4LHT2tqdmG|ZTDBd5NigQ>? NGrmSnI5OM7:TdMg MVmxbC=> NHXne2VqdmirYnn0d{BE[XOyYYPlMVMh[WO2aY\peJnDqA>? NXrFSI1COjR|MUSxN|U>
PDL fibroblasts M{LBbWtqdmG|ZTDBd5NigQ>? MXS4NO69VcLi Mn65NYg> NUjuT|UxcW6qaXLpeJMhS2G|cHHz[U0{KGGldHn2bZR6yqB? NFixe48zPDNzNEGzOS=>
MB49 NHXKPHBCeG:ydH;zbZMhSXO|YYm= Ml32NlDPxE1? NHXiUXcycA>? MmTidoV3\XK|ZYOgR20ucW6mdXPl[EBk\WyuIHTlZZRp NIrMPZMzPDJ6MkSzNy=>
5637 MoLoRZBweHSxc3nzJGF{e2G7 NVnuNlhiOjEQvF2= MnLENYg> NHjwVFlz\X[ncoPld{BEVS2rbnT1Z4VlKGOnbHyg[IVifGh? MkS1NlQzQDJ2M{O=
A549  NFz5c5FCeG:ydH;zbZMhSXO|YYm= NHPRXpEyODEQvF2= Ml3SOFhp NWf0VXdre3WycILld5NmeyC2aHWgZZBweHSxc3nzJINifXOnZDDifUBxcXCncnnu[eKh M1zaXVI1Ojd{MkCx
MCC-2 NXzQbmZ{SXCxcITvd4l{KEG|c3H5 MnLBNlAh|ryP NH;4bo43cA>? MWTEUXNQ NIrtVVNjdG:la4OgbY5lfWOnZDDifUB{fGG3cn;zdI9zcW6nIHHwc5B1d3OrczDj[YxtKGSnYYTo NXPxUWVVOjR{NkK2OVg>
ADF M3e1[GFxd3C2b4Ppd{BCe3OjeR?= M{K2RlQx|ryP M2rSZ|Zp MlHrbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkh[2inbX;0bIVz[XC7IIDseZMhYm6FbEK= MnXSNlQzOjh{M{K=
 U373  MYPBdI9xfG:|aYOgRZN{[Xl? MWe0NO69VQ>? M{nIXlZp Mn36bY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkh[2inbX;0bIVz[XC7IIDseZMhYm6FbEK= M{TUV|I1OjJ6MkOy
RKO-HIPK2i M2HuN2Fxd3C2b4Ppd{BCe3OjeR?= M{HyZVQx|ryP MXq2bC=> MUjpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBkcGWvb4To[ZJieHlicHz1d{BbdkOuMh?= NXfJNYpvOjR{MkiyN|I>
HEC-1B MmL1RZBweHSxc3nzJGF{e2G7 Ml:yNlDPxE1? Mn3VNYg> Ml:0doVlfWOnczDUVE1qdmS3Y3XkJIFxd3C2b4Ppd:Kh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUm= M2PMSVI1OjF|M{W4
4T1 NWD3[XBvS2WubDDWbYFjcWyrdImgRZN{[Xl? NXXYNpFqOi53LUGw{txO MonzOIg> MmnjSG1UVw>? NXP5dllkemW|Y4Xld{B1cGViY4n0c5RwgGmlaYT5JI9nKDSWMTDj[YxteyCrbnT1Z4VlKGK7IGPQSHQhcW5iYTDjc45k\W62cnH0bY9vKGSncHXu[IVvfCCvYX7u[ZI> NGjXXm8zPDJyNkG2NS=>
OS  NHvBd|ZCeG:ydH;zbZMhSXO|YYm= Mn[3NlDPxE1? MYm0bC=> MXvpcohq[mm2czD0bIUh[2WubDDk[YF1cCClYYXz[YQh[nliUFTU MUCyOFIxPDl|Nx?=
RPE NYK4bVJoSXCxcITvd4l{KEG|c3H5 M{LX[|ExOM7:TR?= NEDPUZY1QGh? MWnwZZJ1cWGubImgbY5pcWKrdIOgeIhmKGOjbIDhbY4uOSCjbnSgMVIh[WO2aY\heIlwdiCjczD3[YxtKGG|IITo[UBk[XOyYYPlJIFkfGm4YYTpc44> NEL0OnMzPDJyMkC1Ni=>
UD29a NFLMN4lCeG:ydH;zbZMhSXO|YYm= Mn\yOVDPxE1? M3fEflI1cA>? NEPJT2tqdmirYnn0d{B1cGViY3XscEBl\WG2aDDjZZV{\WRiYomgUnVVOw>? M1nrZ|I1OTlyNUe0
SH-SY5Y  NWHve|JWSXCxcITvd4l{KEG|c3H5 NWPUfoh{OTEQvF2= MVOyOIg> NEHne4lifHSnboXheIV{KGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KEiQRT2ySGc> M{TNSFI1OTR3NE[z
HeLa  NGXVcnVCeG:ydH;zbZMhSXO|YYm= NGDW[HoyOC9{MN88US=> M2DaUFI1cA>? MmLPbY5pcWKrdIOgeIhmKGGldHn2bZR6KG:oIITo[UBu[Wqxcnn0fUBw\iC2aHWgcYVu[mW{czDv[kB1cGViY3HzdIF{\S2oYX3pcJk> MWCyOFE{PzJ4Nh?=
SCCVII  NWniWYJJSXCxcITvd4l{KEG|c3H5 M1zFT|I2|ryP MmqzNYg> NGTwOJRqdmirYnn0d{B1cGViY3XscEBscWyuaX7nJIFnfGW{IHThd4F1cW6rYh?= Mn35NlQyOjZ2NkS=
RAW 264.7 M3XUd2Fxd3C2b4Ppd{BCe3OjeR?= MojjNlDPxE1? MVWxPIg> MmHZbY5kemWjc3XzJGxEOy2LST1Otk1i[3SrbjDyZZRqdyClb33wZZJm\CC2bzDFR3RXNU2RUzD0doVifG2nboSgc45tgQ>? NYHiU3M1OjRzMU[3NFc>
A549  M3Ow[WFxd3C2b4Ppd{BCe3OjeR?= MmrnNk42|ryP NYfufnB6OjSq MVzk[YNz\WG|ZYOgc5Jq\G:waX6tbY5lfWOnZDDheZRweGijZ4mgZZMhf2WubDDhcoQhVG:|czDv[kDPnM,KbTDhcJNwKG:lY4XydoVlKGS3cnnu[{BifXSxcHjh[4lkKHC{b3Pld5M> Ml65NlQyODJ3MkK=
UM-SCC-10A  NW\OTotzSXCxcITvd4l{KEG|c3H5 MXO1NOK2VQ>? MkG0Nog> NHG0c|Rz\WS3Y3XzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGirZ3it[I9{\SCrc3;hcIFvfG:uYXP0c45m NYC1[XMxOjRyOUi3OVM>
BGC-823 M121VGFxd3C2b4Ppd{BCe3OjeR?= MmnnNVAh|ryP M333UlI1cA>? NFi4Z41z\WS3Y3XzJJRmfHKjbnTybY5mNWmwZIXj[YQh[XCxcITvd4l{yqB? M3zTNlI1ODl6NUGx
Nalm-6  MoPORZBweHSxc3nzJGF{e2G7 MnyyNlDPxE1? MnrvOIg> M2DET4lvcGmkaYTzJINie3Cjc3WtPEBidmRiY3HzdIF{\S1|IHHjeIl3[XSrb36gZY5lKFCDUmCtNUBkdGWjdnHn[S=> NFPp[I0zPDB|OUm2Oy=>
KHOS  NHq5SG9CeG:ydH;zbZMhSXO|YYm= Ml;iOFDPxE1? NX;SbGNDOjSq M4ThfoJtd2OtczD0bIUhcW6lcnXhd4VlKGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzDhcoQhWEGUUDDpcoR2[2WmIHL5JGJDVUR|wrC= NFLiW|YzPDB{NUO2NS=>
G292 MXXBdI9xfG:|aYOgRZN{[Xl? MYO0NO69VQ>? MX2yOIg> MonmZoxw[2u|IITo[UBqdmO{ZXHz[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgRmJOTDQEoB?= Moq0NlQxOjV|NkG=
MG-63 Ml;aRZBweHSxc3nzJGF{e2G7 MYi0NO69VQ>? M3\4bVI1cA>? NX;GSFZF[myxY3vzJJRp\SCrbnPy[YF{\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZT2zJIFv\CCSQWLQJIlv\HWlZXSgZpkhSkKPRERCpC=> M2XwfFI1ODJ3M{[x
EBV-transformed B cells NXzUXpBbSXCxcITvd4l{KEG|c3H5 M4WzZVIx|ryP NUS4XodOOmh? MXPEUXNQ MmTsZoxw[2u|IHHueIkuS0R6MDDhcoQh[W62aT3DSFg3KGGwdHnic4R6NWmwZIXj[YQh[XCxcITvd4l{ MXyyOFAxQDZ{OB?=
IM-9 B NF32VYtCeG:ydH;zbZMhSXO|YYm= M4G0VVIx|ryP NVT5bXdGOmh? NH\3[I9FVVOR NGjjSZFjdG:la4OgZY51cS2FREiwJIFv\CCjboTpMWNFQDZiYX70bYJw\HlvaX7keYNm\CCjcH;weI9{cXN? NE\XcFYzPDByOE[yPC=>
CNE1 NF;3[ZFCeG:ydH;zbZMhSXO|YYm= MVOyNO69VQ>? NITUSFI1QGh? NX\vWYdY[myxY3vzJGxMNUFvaX7keYNm\CCjcH;weI9{cXN? NIHiRmMzOzl6NUCyPS=>
CNE2 NH3WT|NCeG:ydH;zbZMhSXO|YYm= MlvNNlDPxE1? MYG0PIg> M3;yW4Jtd2OtczDMT{1CNWmwZIXj[YQh[XCxcITvd4l{ M3ewcFI{QTh3MEK5
HL-60 NFq1RlNCeG:ydH;zbZMhSXO|YYm= M3fXTFUxyrWP MkW3NYg> MV;EUXNQ NH3hd2pqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSCEQUG0OS=> MWeyN|k1QDd3MR?=
L929 NWLEcZdKSXCxcITvd4l{KEG|c3H5 MljqNU4zPeLCk{ZCpO69VcLi NFjFOGYzPGh? NFfUU2tifWevZX70d{BVVkcQsT3pcoR2[2WmIH7lZ5JweHSxc3nzJIFv\CCjdYTvdIhi\3l? Mn\ZNlM6PDF5Nkm=
Jurkat M4ezPGNmdGxiVnnhZoltcXS7IFHzd4F6 NVLGOnJ2Os7:TR?= NXTJcYJXPGh? NXiyVos2emW4ZYLz[ZMh\3Kxd4ToJIlvcGmkaYTpc44h[W6mIN8yMYNifGWwaX6g[IVkemWjc3WgZpkhUFNvQWPB NWrPR5JPOjN6OU[wOlE>
RAW264.7  NXXybmJtSXCxcITvd4l{KEG|c3H5 NGTmN|gyOCEQvF2= NFXZNoYzPGh? Ml:z[IVkemWjc3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDHRS=> MoLHNlM5OjB{MEO=
A431  MWXLbY5ie2ViQYPzZZk> MVy0NO69VQ>? M2XTXVJp M3;SeYJtd2OtczDmbZNmfGmwLXnu[JVk\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZYOgZY5lKFCDUmC= NHyzcI8zOzhyMEC1PC=>
Jurkat M{O3ZmNmdGxiVnnhZoltcXS7IFHzd4F6 M3XpOlI2NTFyMN88US=> MorHOog> MojNbY5pcWKrdIOgfk1HSS2FTVutbY5lfWOnZDDj[YxtKGSnYYTo NYfuRotiOjN7M{O1N|I>
COS-7  NX;xeYZZTnWwY4Tpc44hSXO|YYm= MWC1NOK2VQ>? NYHkV41YOjSq MWPh[oZm[3S|IITo[UBxem:lZYPzbY5oKG:oIFHUUlF{KHerdHigdI9tgVFidILhZ5R{ M3O1T|I{QTN|MkC4
CAL27 NH7ZU2xCeG:ydH;zbZMhSXO|YYm= NVjXboE5OTBizszN Mnn0Nog> M2Dt[pN2eHC{ZYPz[ZMhS3W{LV7Qd{1z\WS3Y3XkJJZq[WKrbHn0fUBjgSC3cDD0c{A6OCV? MlX3NlM6OTd|OU[=
THP-1  NGXNO4xCeG:ydH;zbZMhSXO|YYm= NEXEbnMyOC13MN88US=> M4qw[|Jp MUnpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUB1emmydH;sbYRm NGTveVQzOzlyMEK5PS=>
K562 MkXTRZBweHSxc3nzJGF{e2G7 NInPO48zOM7:TR?= Mn\POFhp Mk\ZbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhPC2PVR?= NXXudJBSOjN6N{[4NlY>
HL-60 MWLBdI9xfG:|aYOgRZN{[Xl? MmrXNVAx|ryP MXuyOIg> NYnmU45IcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliYXLp[ZRidmViZHn0[ZJx\W6nc9Mg NILPbWMzOzh4NUe3PC=>
HeLa  M3XaWWFxd3C2b4Ppd{BCe3OjeR?= NInQ[3UzOM7:TR?= NXewNY9FOmh? NUT0RmpKcW6qaXLpeJMhTE2PUDDpcoR2[2WmIHHwc5B1d3Orcx?= NGPGU5QzOzh4M{m2Oi=>
U251 NF7rVFZCeG:ydH;zbZMhSXO|YYm= M33yZVIx|ryP NVfrNXJLOjSq MVXpcohq[mm2czDjZZN1cWOrbjDpcoR2[2WmIFeyM20heGijc3WgZZJz\XO2IHHu[EBieG:ydH;zbZM> NFPJRYMzOzhzNkixOi=>
HL-60 M{j2RWFxd3C2b4Ppd{BCe3OjeR?= M2f1dlUxyrWP M4O5XVRp M1T4eIJtd2OtczD0bIUh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|LDCtPUwh[W6mIGDBVnAhcW6mdXPl[EBjgSB4cx?= NIPpdmczOzhyNEewOi=>
Ec-109 NH64Z3NCeG:ydH;zbZMhSXO|YYm= M3XUZ|ExKM7:TR?= NEL3fJc1QGh? MXjy[ZBz\XO|ZYOgVGUudWWmaXH0[YQhTWNvMUC5JINmdGxiYYDvdJRwe2m| NXnaOGEzOjN5OEK2OFE>
RKO MnXiR4VtdCCYaXHibYxqfHliQYPzZZk> M3jpS|ExKM7:TR?= M13aTlI1cA>? Ml\5bY5pcWKrdIOgeIhmKGSnY4LlZZNmKG:oIHPlcIwhfmmjYnnsbZR6KGOjdYPl[EBjgSCGQVK= NGry[pkzOzd3OEC2OC=>
T98G M3XNVWNmdGxiVnnhZoltcXS7IFHzd4F6 MmrWNU0yODEQvF2= NHeyWmwzPGh? NIPsc5NqdXC{b4\ld{Bk\WyuII\pZYJqdGm2eTDjc5Rz\WG2bXXueEB4cXSqIGTT MkXBNlM4Pjl{N{W=
Jurkat MlnjR4VtdCCYaXHibYxqfHliQYPzZZk> NITHT2MyODBvMkCw{txO MmDMNlRp MV;pcohq[mm2czDIZWE1KGmwZIXj[ZMh[XCxcITvd4l{KGSncHXu[IVvfCCxZjDjc45k\W62cnH0bY9vyqB? MX2yN|c{OjR6MR?=
Molt-3  M1HHOWFxd3C2b4Ppd{BCe3OjeR?= NXX0bGltPTEQvF2= NWDFUXJ6Omh? MkfSdoVlfWOnczDt[YxifG:waX6tbY5lfWOnZDDhdI9xfG:|aYO= MUeyN|czPTBzMx?=
hMSC12 M2LYbmFxd3C2b4Ppd{BCe3OjeR?= M2LCN|ExOM7:TR?= MlvWOIQ> NHfoN5ZqdmirYnn0d{Bwe3Snb3flcolkKGO3bIT1doUucW6mdXPl[EBk\WyuIHTlZZRpKGGwZDDjZYxkcW[rY3H0bY9v M2LlU|I{PjV5OEKy
HM7  Moq0RZBweHSxc3nzJGF{e2G7 MX2yNO69VcLi NXLmeJozOWh? NVe4Um1O[myxY3vzJIFxcWO3bHHy[Y4hSSCjY3X0ZZRmNWmwZIXj[YQh[2G|cHHz[U0{KGGldHn2ZZRqd25iYX7kJHBCWlBiY3zlZZZi\2V? MnjPNlM2QDN2MUK=
Hep-2  MonIRZBweHSxc3nzJGF{e2G7 MlXsNlDPxE4EoB?= M{DtTFI1cA>? MnvHSG1UVw>? NVXLfZZX[WyuZY\pZZRmeyC2aHWg[IVkemWjc3Wgc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUB{cWyrYnnubY4> MnnpNlM2QDByM{K=
HSCs M3vRfmFxd3C2b4Ppd{BCe3OjeR?= NFLG[4s2OM7:TR?= M2TjNlI1cA>? Mke3SG1UVw>? NGrNcZRqdmirYnn0d{BvcWyxdHnubYIucW6mdXPl[EBFVkFiZHHtZYdmKGmwZHnjZZRm\CCkeTDQRXJRKGOuZXH2ZYdm MX:yN|Q6QTh5NB?=
HL-60  NFrSSpJEgXSxdH;4bYNqfHliQYPzZZk> Ml\rNlDPxE4EoB?= Mk\PNlRp MYLy[YR2[2W|IITo[UBkgXSxdH;4bYMh\W[oZXP0JI9nKEW{eUW= MYeyN|Q6PDR6MB?=
HA M1rrO2Fxd3C2b4Ppd{BCe3OjeR?= NIL0V2s2OM7:TR?= MmTONlRp MVTzeZBxemW|c3XzJJRp\SClbHXheoFo\SCxZjDQRXJRKGGwZDDjZZNx[XOnLUOsJE04NCCjbnSgMVkhcW6mdXPl[EBjgSCkb4L0[ZpwdWmkL2PBTGE> Moj2NlM1PzV7NU[=
C666-1 NEjIcZRCeG:ydH;zbZMhSXO|YYm= MWi1NO69VQ>? NYXaUYdCOjSq M3m3epN2eHC{ZYPz[ZMhfGinIHPs[YF3[WenIH;mJHBCWlBiYX7kJINie3Cjc3WtN{whNTduIHHu[EAuQSCrbnT1Z4VlKGK7IHLvdpRmgm:vaXKvV2FJSQ>? NHjxZlUzOzR5NUm1Oi=>
Hepa1-6 MXrBdI9xfG:|aYOgRZN{[Xl? M{S3OlUx|ryP MmjENog> M4DHb4lvcGmkaYTzJIN2emO3bXnuJIFv\CC{ZYP2[ZJifHKxbD3pcoR2[2WmIHHwc5B1d3Orcx?= NVzneWZlOjN2NE[3OVM>
PLC/PRF/5c M1vtXmFxd3C2b4Ppd{BCe3OjeR?= M3PiU|Ux|ryP NHzoWpAycA>? M{jWcpBz\X[nboTzJIFxd3C2b4Ppd{B1emmpZ3Xy[YQh[nliQ1nO M4fjfVI{PDN6OEK0
HCT116 M1;LVWFxd3C2b4Ppd{BCe3OjeR?= MkDzNVAx|ryP NUSxXnpjOS53aB?= NV\tTVFJcW6qaXLpeJMhV3NvaX7keYNm\CClZXzsJIRm[XSq MY[yN|c1PDN3Mx?=
HCEC NWXsfGd{TnWwY4Tpc44hSXO|YYm= M2LZe|Ux|ryP MWC3Nog> M4fv[GROW09? M{\XTpJme3SxcnXzJI9nKHSqZTDuc5Ju[WxiSFPFR{BxcGWwb4T5dIU> M2LBXVI{PzR{MEGx
Primary rat cerebral cortical neurons MYHBdI9xfG:|aYOgRZN{[Xl? MXGxNFDPxE1? NIfvTJYycA>? M{DXZZBz\X[nboTzJGNlNWmwZIXj[YQh[XCxcITvd4l{KGGwZDDj[YxtKGSnYYTo MV6yN|c1OTNzNx?=
MDA-MB-231  M3rqdWFxd3C2b4Ppd{BCe3OjeR?= Moj3NlXPxE1? Mn7aNlRp NVuxb|RY[WK{b3fheIV{KGO7dH;0c5hq[2m2eTDhcoQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgVoc{ NHLMWZEzPTN|N{W0OC=>
NLRP3-Tet-on-MC/9  NYXpZpM3SXCxcITvd4l{KEG|c3H5 MmPSNVAuPDEQvF2= NXPqNHhvOTKq MWnheJRmdnWjdHXzJJRp\SClZXzsJIRm[XSqIHPheZNm\CCkeTDDRXBUNWG|c3;jbYF1\WRiTlzSVFMhdXW2YX70d{BqdiC2aHWgWIV1NW:wIIP5d5RmdQ>? MoDMNlM4ODN|OEm=
KNS42  M17jZWFxd3C2b4Ppd{BCe3OjeR?= NYDqV2twPTEQvF2= MortNYg> MUTy[YR2[2W|IHPlcIwh\GWjdHigZY5lKGOxbYDs[ZRmdHliYXLvcIl{cGWmIHPhd5Bie2ViMz:3JIFkfGm4aYT5JIlvKHKnc4DvcpNmKHSxIFHCWE0zPjNxMlTHM41mfG[xcn3pckBkd22kaX7heIlwdg>? M2exfFI{PjlzMUS1
MCF-7  Mo\hRZBweHSxc3nzJGF{e2G7 M{POVFIx|ryPwrC= MmfMO|Jp MW\pcohq[mm2czDldZVwdC1iYX7kJFQuV0iWLX3l[IlifGWmIHHwc5B1d3Orcx?= MXeyN|Y4PTZ2Mx?=
hCMEC/D3  MkHzRZBweHSxc3nzJGF{e2G7 NGXvNXQzPc7:TR?= MX23Nog> MUDy[YR2[2W|IFz0fGEhcW6mdXPl[EBieG:ydH;zbZM> M4TLWlI{PjZ3MUm4
Jurkat NWr5PZExSXCxcITvd4l{KEG|c3H5 MVGxNk42NTVyzszN MWWxbC=> MlrV[I9{\SCmZYDlcoRmdnSueTDzeZBxemW|c3XzJHNRUC2rbnT1Z4VlKFCjcj20JINt\WG4YXflMEBRSVKSIHPs[YF3[WenLDDEUmEh\nKjZ33lcpRifGmxbjygZY5lKGyxc4Ogc4YhfmmjYnnsbZR6 MXmyN|Q1Ojl5Nh?=
CNE-1 NVLQN3FTSXCxcITvd4l{KEG|c3H5 NUHDZYlkOjEQvF5CpC=> MWmyOIg> MlXsbY5pcWKrdIOgVmFFODBzLXnu[JVk\WRiY3XscEBl\WG2aB?= M2G4ZVI{PDJ4OEWw
HONE-1 MX;BdI9xfG:|aYOgRZN{[Xl? MkLXNlDPxE4EoB?= MkDtNlRp NFzDWZRqdmirYnn0d{BTSURyMEGtbY5lfWOnZDDj[YxtKGSnYYTo MVqyN|QzPjh3MB?=
astrocytes cell M3XGZ2Fxd3C2b4Ppd{BCe3OjeR?= MnzzOFDPxE1? M1i5WlZp MmL2doVlfWOnczDlZZJtgSCjcH;weI9{cXNiaX7keYNm\CCkeTDzeIF2em:|cH;ybY5m Mk\aNlM1OTF5N{i=
U-937 NGDDSoRCeG:ydH;zbZMhSXO|YYm= MUWxNO69VQ>? MmnWN|BucW5? NF\4VlVxemW4ZX70d{BVVkZvaX7keYNm\CCwZXPyc5B1d3Orcx?= NEexVVUzOzRzMEe0PC=>
MDA-MB-231 MWDBdI9xfG:|aYOgRZN{[Xl? MXyxNFDPxE1? NHy2[m0ycA>? NXLEWmF2cW6qaXLpeJMhe2Wwc3n0bZpifGmxbjD0c{BVWkGLTDD1dI9vKE2WRFig[I94di2{ZXf1cIF1cW:w MX:yN|QxQDR{OR?=
HeLa  NF63WnJCeG:ydH;zbZMhSXO|YYm= M4TUVFExOM7:TR?= NF3CNXEzcA>? NVfTbXV{[myxY3vzJGpTWy1zNTDJcoR2[2W|IFHwc5B1d3SrYzDD[YxtKESnYYTo MVyyN|M1PDB2NR?=
Ec109 MVfBdI9xfG:|aYOgRZN{[Xl? MYmxNO69VQ>? NV\DZnd{Pmh? MUPicI9kc3NiYYDvdJRwfGmlIHPvcYJqdmG2aX;uJI9nKFSjdD3TcYFkVjdiYX7kJJJi\GmjdHnvci=> MYOyN|M{QDV4OB?=
H460  M1XRO2Fxd3C2b4Ppd{BCe3OjeR?= NX\3PG5JOTEQvF2= NV3CN3djPmh? NVXQZphP[myxY3vzJIFxd3C2b4TpZ{Bkd22kaX7heIlwdiCxZjDUZZQuW22jY163JIFv\CC{YXTpZZRqd25? NIH3emszOzN|OEW2PC=>
HeLa  MWrBdI9xfG:|aYOgRZN{[Xl? M4rNWlUx|ryP MWWxMlVp MlTmZYJzd2ejdHXzxsBEcGyjbYnkbYEucW6mdXPl[EBieG:ydH;zbZM> MYWyN|MxOzhyNB?=
SK-HEP1 MnHoRZBweHSxc3nzJGF{e2G7 NUfFeXVYOTByzszN MWCxbC=> NXLxTWg3cW6qaXLpeJMhS3KWMTDhZ5RqfmG2ZXSgZ4F{eGG|ZT2zMEAuPyxiLUisJE06NCCjbnSgdI9tgSiDRGCtdoljd3OnKTDwc4x6dWW{YYPl M1PjNlI{OzB{NkWw
QGY7701 MkPXRZBweHSxc3nzJGF{e2G7 NXT5cFVnOjYQvF2= MUmxMlVp NEXjW5BqdmirYnn0d{Bi[2O3bYXsZZRqd25ib3[gd5VjNUdzIIDoZZNmKGmwZIXj[YQh[nliRF;YJEsheXWncnPleIlv MoL2NlMzPDByNkG=
HepG2 MlfMRZBweHSxc3nzJGF{e2G7 M2TJUFIx|ryP MWSzNI1qdg>? NYXyRmVUcW6qaXLpeJMhfGinIHXubIFv[2WmIHPlcIwh\GWjdHigZpkh[2:vYnnu[YQhfHKnYYTt[Y51KG:oIHHwbYdmdmmwIHHu[EBVWkGLTB?= NUTGRnY4OjN{MkSyN|k>
U87  M{nHfmFxd3C2b4Ppd{BCe3OjeR?= NGXEcXUzPc7:TR?= M2\4b|Jp MoHS[IVkemWjc3XzJIl{d2yrcYXpdol1cWenbnnuJEhKW0xrLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHisJIJ2fCCwb4SgcoVkem:2aXOgZ4VtdCCmZXH0bC=> NXrsNm5COjN{Mkm2NlY>
HSC-2 Mn;5RZBweHSxc3nzJGF{e2G7 NULHR2pROjVxNUFOwG0> Ml6wNYg> M4[1XYlvcGmkaYTzJHNPNTN6LXnu[JVk\WRiY4n0c5RwgGmlaYT5 NGHJNoYzOzF3NUK0PC=>
HSC-4 M4LKd2Fxd3C2b4Ppd{BCe3OjeR?= MmPuNlUwPTEQvF2= MorlNYg> MWrpcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?= MXWyN|E2PTJ2OB?=
CL-1 MULBdI9xfG:|aYOgRZN{[Xl? MVSyNO69VQ>? MmjpNYg> MXPicI9kc3NiT2C0OFkhcW6mdXPl[EBk\WyuIHTlZZRp MmDaNlMyOzF5OEK=
MEL M1[yVmFxd3C2b4Ppd{BCe3OjeR?= NUDrSpFpOjEQvF2= NWfGZ3hJOjSq NXznbXBCTE2VTx?= MnfGbY1x[Wm{ZYOgVFJZPy2rbnT1Z4VlKE2HTDDj[YxtKGGyb4D0c5Nqew>? M1PxcFI{ODF2OEi3
Bel-7402  NF7HXJFE[XOyYYPlJGFkfGm4YYTpc44hSXO|YYm= MUS1NO69VQ>? MkH4Nog> MnnXbY5pcWKrdIOgVGNGNWmwZIXj[YQh[W6xaXvpdy=> NW[0UpJLOjNyMEi3OFI>
Eca-109 NFG3d4NCeG:ydH;zbZMhSXO|YYm= MYGyOe69VQ>? M2rFSVMxdWmw NV3oSYltcW6qaXLpeJMhSkpvQkGxMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NVr3XG1rOjNyN{[5Olc>
MEL MkjQRZBweHSxc3nzJGF{e2G7 MX2yNO69VQ>? MUKxbC=> M1S5VWROW09? MkfHbY1x[Wm{ZYOgVFJZPy2rbnT1Z4VlKE2HTDDj[YxtKGGyb4D0c5Nqew>? M33DdlI{ODF2OEi3
Bel-7402  MVXBdI9xfG:|aYOgRZN{[Xl? NEjM[XU2OM7:TR?= M3KwOFJp MlnEbY5pcWKrdIOgVGNGNWmwZIXj[YQh[W6xaXvpdy=> NFTZZ3QzOzByOEe0Ni=>
L929 NUfYWWxSTnWwY4Tpc44hSXO|YYm= M{H5c|IvPc7:TR?= NIjidW8ycA>? NHPBSoVqdmO{ZXHz[ZMhWkmSMTDlfJBz\XO|aX;uJIFv\CCneHHj[ZJj[XSnZDDUUmbPuS2rbnT1Z4VlKG2rdH;jbI9v\HKrYXyg[Jl{\nWwY4Tpc44h[W6mIGLPV{Bxem:mdXP0bY9v NI\Cc4czOzByMEWxPC=>
RCC  MmezR4VtdCCYaXHibYxqfHliQYPzZZk> MYGxNFDPxE1? M3LpVlI1cA>? MX3y[YNwfmW{ZYOgeIhmKH[rYXLpcIl1gSCxZjDj[YxteyCneIDvd4VlKHSxIEG1[E1RT0p{ MVOyNlk6OTR7NB?=
NB2a/d1 MYHBdI9xfG:|aYOgRZN{[Xl? NX;uS2dPOTByzszN M3LSRVczcA>? NEHHfW5ifHSnboXheIV{KHO2YYXyc5Nxd3KrbnWtbY5lfWOnZDDjZZNx[XOnIHHjeIl3cXS7LDDQRXJRNCCjbnSgeIF2KGOuZXH2ZYdm NFfhZWQzOjl6OEW0NS=>
T cell MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXKyOU0yODEQvF2= M3HZ[|I1cA>? MV3kc5NmNWSncHXu[IVvfGy7IHnubIljcXSnZDDUJINmdGxicILvcIln\XKjdHnvckBu\WSrYYTl[EB1cHKxdXfoJJRp\SClbz3zeIlufWyjdHnvckB4cXSqIHHueIkuS0R|IHHu[EBidnSrLVPENlg> NEGxU3UzOjl6MkWzPC=>
K562 M4XWO2Fxd3C2b4Ppd{BCe3OjeR?= MoPSNVAx|ryP MnfrNYg> M3fLdIJtd2OtczDBZo5w[mG4aYPjeY3DqEZvaX7keYNm\CCjcH;weI9{cXN? NFHLVGszOjl5MkO3Ni=>
Jurkat  MmDZRZBweHSxc3nzJGF{e2G7 M{PLeFQx|ryP MXGxbC=> NUjaRoti[WKxbHnzbIV{KE[jc1ytbY5lfWOnZDDjZZNx[XOnIHHjeIl3[XSrb36gZY5lKGOnbHyg[IVifGh? MnPENlI6PDJ5M{i=
BGC-823 NXTx[pRkSXCxcITvd4l{KEG|c3H5 NULsfoJyOTByzszN NEjjNZAycA>? MWDwZZJ1cWGubImgdoV{[3WnczDj[YxteyCjZ3HpcpN1KGSjbXHn[UBw\iCmYXnkfoVqdg>? MojXNlI6OjZ3NEW=
Hep3B MYTBdI9xfG:|aYOgRZN{[Xl? Ml7UOVDPxE1? MYqxbC=> M2T5N4Jtd2OtczDhdI9xfG:|aYOgbY5lfWOnZDDifUBJTUeFcx?= MYeyNlkzOzF3NB?=
LLC-PK1 NEjJOYRCeG:ydH;zbZMhSXO|YYm= NXO2UYJDOjEQvF2= MYqxbC=> M1vUcJBz\X[nboTzJINqe3CuYYTpck1qdmS3Y3XkJIRm\3KjZHH0bY9vKG:oIFH0[|UtKGKnY3zpck0yNCCjbnSgRZRoOTJicILveIVqdnN? M{jQOlIzQDl4MEO3
A549 M1O1emFxd3C2b4Ppd{BCe3OjeR?= MUm1NO69VQ>? MlPkNYg> NFPNcIZjdG:la4OgeIhmKEKDST3pcoR2[2WmIHHwc5B1d3Orc9Mg M2C0eVIzQDh5MkG1
SGC-7901  M13IOWFxd3C2b4Ppd{BCe3OjeR?= NVHmdFNEOTEQvF2= MX6yOIg> NEnENJNqdmirYnn0d{DPui{Qsj3kbY1mfGi7bHHjdplte2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZPDqA>? NYTWclF5OjJ6NEi1PVc>
DM6 MmnLRZBweHSxc3nzJGF{e2G7 MofPNVAx|ryP MXG3Nog> MV3icI9kc3NiYn;0bEBGOkZvMTDhcoQhTTKIdIKtcYVlcWG2ZXSgZ5l1d3SxeHnjbZR6 NUjTXZF{OjJ6MkWzNlg>
MCF-7, MDA-MB-468, Caco-2 NUC2WpZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrTOVDPxE1? NYXDTlg1PDiq MVTpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JJNieG:waX6= NIP2Z4szOjhyMEm2PC=>
A2750  NIPuSG1CeG:ydH;zbZMhSXO|YYm= M2LIWVIx|ryP M2rG[VJp NX:xWZhTTE2VTx?= MlniZoxw[2u|IHPhd5Bie2ViY3zlZZZi\2ViZIXybY5oKGinbHXuZYxqdiC2cnXheI1mdnRiYX7kJJJm\HWlZYOgZZV1d3CqYXfpZ{Bk\WyuIHTlZZRp M37LVlIzPzh2M{[z
U87  MmLQRZBweHSxc3nzJGF{e2G7 MonwNlDPxE1? M2OwUlI1cA>? MUHy[YR2[2W|IITo[UBieG:ydH;zbZMhemG2ZTDpcoR2[2WmIHL5JHBNSUJ? MV:yNlc4QDd6MB?=
HT1080 NHLDT2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HocFUx|ryP M1nlS|dl M1HBSYlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDpcohq[mm2aX;uJINifXOnZDDifUBkd22kaX7l[EB1emWjdH3lcpQhd2ZiRFPBJIFv\CCRTWC= MYGyNlc1ODl6NB?=
A549  M2HBOmFxd3C2b4Ppd{BCe3OjeR?= NW\SNJZwPTEQvF2= M2TsdFJp Mn[xdIFzfGmjbHz5JIRm[3KnYYPld{B{d2SrdX2gd4Vt\W6rdHWtbY5lfWOnZDDhdI9xfG:|aYO= NFLmWJUzOjd{MUiwOC=>
Primary OPC MVXBdI9xfG:|aYOgRZN{[Xl? NEWzUXcy|ryP M2\IWFZpNzJ2aB?= NWDJXJJnemWmdXPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBm[XKueT2gZY5lKGyjdHWtZZBweHSxc3nzM45m[3Kxc3nz M1GxW|IzPzB5M{i1
PMNs MUjBdI9xfG:|aYOgRZN{[Xl? NFfzc5Y1OM7:TR?= MVK2bC=> MoW1SG1UVw>? NX7weZZMemW4ZYLz[YQhfGinIHHtc5VvfCCxZjDjcIVifmWmIHPhd5Bie2VvMzD0c{Bv\WG{II\lbIlkdGVibHX2[Yx{ NH75VY4zOjZ7MkW3Oy=>
A549  M37kZmFxd3C2b4Ppd{BCe3OjeR?= NYLT[oFzPTEQvF2= Mn7aNYg> MUPwdoV3\W62czDhdI9xfG:|aYOgbY5lfWOnZDDifUBD[W:qdX;zbYRmKEl? NX7U[Ip4OjJ4OEe2N|U>
AGS MYXBdI9xfG:|aYOgRZN{[Xl? MYqyNO69VQ>? NVPGWFZuOTKq MUXpcohq[mm2czD0bIUh[WO2aY\heIlwdiCxZjDwdo8u[2G|cHHz[U0{KGmwIILld5BwdnOnIITvJJRp\SCHdF;BZ{BnemGldHnvci=> MV[yNlY5PzN7OB?=
shC9  NV3Zb5hzSXCxcITvd4l{KEG|c3H5 M2XGZ|Ex|ryP NWDReYpqOT[q MWPicJVvfHNiU1jIJIV5eHKnc4Ppc44hcW5ic3jDPUBk\WyuczDlfJBwe2WmIITvJIVqfGincjDQRUBweiCOUFO= Mmm2NlI3PDFyOUS=
primary MEFs MlHaSpVv[3Srb36gRZN{[Xl? MXGxNog> MWFCpIlv[3KnYYPld{BmfG:yb4Pp[IUucW6mdXPl[EBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIHTldI9t[XKrenH0bY9v NV\rbGk4OjJ4MUO3Olc>
3T9 MEFs NXHBR3d[TnWwY4Tpc44hSXO|YYm= M37uNFE3cA>? NGfMVY5qdmO{ZXHz[ZMh[3m2b3Podo9u\SClIILlcIVie2ViYX\0[ZIh\XSxcH;zbYRmNXS{ZXH0cYVvfA>? MUKyNlYyOzd4Nx?=
3T9 MEFs NX7ifpFwTnWwY4Tpc44hSXO|YYm= NEO1S3EyQGh? MYH1dJJm\3WuYYTld{Bqdmm2aXH0c5Ih[2G|cHHz[U06KGK3dDDkc5dvemWpdXzheIV{KGWoZnXjeI9zKGOjc4Dhd4V{KGGodHXyJIV1d3Cxc3nk[UB1emWjdH3lcpQ> MYWyNlYyOzd4Nx?=
MDA-MB-231  MVrBdI9xfG:|aYOgRZN{[Xl? M3nGSVIx|ryP M2L2clRl NVPZRmRIemWmdXPld{B1cGViY3XscEBieG:ydH;zbZMhcW6mdXPl[EBjgSCvZYTmc5JucW5idILlZZRu\W62IIfhd{B{cWewaX\pZ4FvfGy7 MnTxNlI2QTN2NEG=
C6  M1f1b2Fxd3C2b4Ppd{BCe3OjeR?= MlXuNVDPxE1? NEixd5QzPGh? M1HXTIJtd2OtczD0bIUhe3WycILld5NqfmViZX\m[YN1KG:oIITo[UBx\XC2aXTlJI9vKH[rYXLpcIl1gQ>? M1rLOlIzPTh6OUiw
HL-60  MYDBdI9xfG:|aYOgRZN{[Xl? M2fibFExOM7:TR?= NUfpc|hIOjSq MlS1bY5pcWKrdIOgeIhmKGOnbHygZZBweHSxc3nzJJNmdGWldHXkJEgsMS2vZX70bJltKM7{LTix5qaTPilvZHnncJVkd3C7cnHuc5Nq\GYEoEW= NInlbZczOjV2Nk[2PS=>
HL-60 M1POPGFxd3C2b4Ppd{BCe3OjeR?= NFfabmEyOC16MN88US=> NXHuO2tYPGh? M3\kNYlvcGmkaYTzJHRIUFFvaX7keYNm\CClZXzsJIFxd3C2b4Ppd:Kh MV2yNlUzOzJ{OR?=
BCC MYTBdI9xfG:|aYOgRZN{[Xl? Mly1OVDPxE1? NUTvc|ZyOWh? NGK1T|JqdmirYnn0d{BFSVSVLX3l[IlifGWmIHfyc5d1cCCrbnjpZol1cW:w NUP4UnBHOjJ3MUm0N|Y>
RAW 264.7 Mk\tRZBweHSxc3nzJGF{e2G7 Moq2OVAwOTByzszN MVOxbC=> M3rUXYNwdmOnboTyZZRqd25vZHXw[Y5l\W62IHnubIljcXS|IFTPUk1qdmS3Y3XkJJJTVkFiY3zlZZZi\2V? NWq1cng4OjJ2OUG0NlY>
K562 NXHDUmJjSXCxcITvd4l{KEG|c3H5 NF\CZoMzPc7:TR?= M{DaflJp NVrhZlNqeHKndnXueJMh[XCxcITvd4l{KGmwZIXj[YQh[nliY3:teJJm[XSvZX70JJdqfGhiYX31doVve2mwIFegZY5lKFSUQVnM NHvRNYEzOjR6M{e3Oy=>
SGC-7901 MVjBdI9xfG:|aYOgRZN{[Xl? MWWyNEDPxE4EoB?= MYeybC=> NXu4SGFo[XS2ZX71ZZRmeyCKMl:yxsBweiCWTl[g{tEucW6mdXPl[EBk\WyuIHHwc5B1d3OrczDhd{B4\WyuIHHzJINie3Cjc3WtN{Bi[3Srdnn0feKh MkPrNlI1PzF3OEm=
PC3 NYP5c|F2SXCxcITvd4l{KEG|c3H5 NF7Q[WMyOM7:TR?= MUG0bC=> MX;jc5VvfGW{czDmcIF3d2OxeHnkMYlv\HWlZXSgZ4F{eGG|ZT3y[YxifGWmIHHwc5B1d3Orcx?= NHHhd2YzOjR5MUm3OC=>
SMMC-7721 M4rnZmFxd3C2b4Ppd{BCe3OjeR?= NIn2NFk2OM7:TR?= NWq3fmF7PDiq NXnVNnJL[XS2ZX71ZZRmeyCxVGKtbY5lfWOnZDDhdI9xfG:|aYO= NHryfHIzOjR4NUizNy=>
HeLa  M2\wRWFxd3C2b4Ppd{BCe3OjeR?= Mnq5OVDDqM7:TR?= M1X6TlQwQGh? NVLWS5BEcW6qaXLpeJMhW1SVLXnu[JVk\WRibHH0[U1xcGG|ZTDhdI9xfG:2aXOg[ZZmdnS| M3nCclIzPDZyNUC0
HeLa  NV3PPVFySXCxcITvd4l{KEG|c3H5 MUC1NOKh|ryP NFK5c|MycA>? M3zrSZN2eHC{ZYPz[ZMhfGinIF\SRXAucW6mdXPl[EBi[2O3bYXsZZRqd25ib3[gZY5v\XirbjDWJJBwe2m2aY\lJINmdGy| MWeyNlQ1QTR2MB?=
T47D  MknWRZBweHSxc3nzJGF{e2G7 MVixNFDPxE1? M2jiUFFp NXHmdm81[myxY3vzJJRp\SCpZX7ldoF1cW:wIH;mJGUu[2GmL1PUSlIh[nliU2TT NF3NclgzOjRyMUG2PC=>
HeLa  NWe4Wm55SXCxcITvd4l{KEG|c3H5 NFHyNWY{OM7:TR?= NHj3T3o1cA>? MURCpIlv[3KnYYPld{B1cGViZ3Xu[ZJidCClZXzsJJZq[WKrbHn0fUA1QCCqIHHmeIVzKHCqb4Tv[Jlv[W2rYzD0doVifG2nboVCpC=> NV3meHhQOjJ|OUSyOFg>
HCC MVjBdI9xfG:|aYOgRZN{[Xl? Mo\BNlAh|ryPwrC= NFn6R2gzcA>? NYfSW2VZ[XS2ZX71ZZRmeyC2aHWgSGhCKGmwZIXj[YQh[WO2aY\heIlwdiCxZjDQRXJR M4nRbFIzOzR{N{Oy
mESCs NWLBU4VJSXCxcITvd4l{KEG|c3H5 M3TWOVIvPc7:TR?= MWGybC=> NIPp[ZRqdmirYnn0d{B1cGViTnHGMY1m\GmjdHXkJINie3Cjc3WgZYN1cX[jdHnvci=> MXOyNlI5PTJ5NB?=
EMT-6  Mmi2R5l1d3SxeHnjbZR6KEG|c3H5 MorJNVAx|ryP NYDxcYhZOWh? NVPPW2FTeGG{dHnhcIx6KGKub3Pr[YQh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IIPpdoFu\XOrbnW= NUHSXmxVOjJ{NUG5NlE>
MCF7 M4HTZmFxd3C2b4Ppd{BCe3OjeR?= NIPud3Q2OMLizszN NHL2V2oycA>? NUH3WVdqcW6qaXLpeJMhWEFiYXP0bZZifGWmIHPhd5Bie2VvMzygMVktKGGwZDDwc4x6MEGGUD3ybYJwe2VrIIDvcJlu\XKjc3W= M2L6WVIzOjJ|M{S1
K562 NUHnUFZnSXCxcITvd4l{KEG|c3H5 MnPDNlAh|ryPwrC= NGTEU2U1QGh? NUniV3JWTE2VTx?= MYficI9kc3NibHHwZZRqdmmkLXnu[JVk\WRiaX7obYJqfGmxbjDv[kB3cWGkaXzpeJkh[W6mIHHwc5B1d3OrczDpcoR2[3Srb36= MVmyNlIyPjF3OB?=
Molt4-hyg MnvvRZBweHSxc3nzJGF{e2G7 MlvjNVDPxE1? NV;LeZl[OC53aB?= NEGzRYZjdG:la4Og[oFzdmW|b3ytbY5lfWOnZDDjZZNx[XOnLUOtcIls\SCjY4Tpeol1gQ>? MUOyOlI4PThzMR?=
HeLa NGnlfmZCeG:ydH;zbZMhSXO|YYm= NYjKfG1DOTEQvF2= MUiwMlVp MV;pcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUB1cGViY3;tZolv\WRidILlZZRu\W62IIfpeIgh\2:vaYPpckBPKGGwZDDUVmFKVA>? NH[xe5gzOjF5OU[2NS=>
Jurkat T MWrBdI9xfG:|aYOgRZN{[Xl? NELkfVU{OM7:TR?= M{\u[VAvPWh? NFr5R4ZFVVOR M3\tWYJtd2OtczD0bIUhgmm{YX2tbY5lfWOnZDDhdI9xfG:|aYO= M33peVIzOTV7OEm4
Neutrophil MlzqRZBweHSxc3nzJGF{e2G7 NVH6NmdsOjEQvF2= NF63fVUxNjWq NVnUdmV[[XS2ZX71ZZRmeyC2aHWgdJJwNWGyb4D0c5Rq[yCnZn\lZ5Qhd2ZiTXHSNS=> MknvNlYyQTZ6NES=
HCT116 M1;sVmFxd3C2b4Ppd{BCe3OjeR?= NV3wWYt4PTEEoN88US=> MmrsNog> NHHhOlZz\X[ncoPld{B{gW6ncnfpd5Rq[yCjcH;weI9{cXNiZX\m[YN1eyCxZjDj[Yxm[2:6aXKgZY5lKE6SQz2xOuKh M4Xp[VIzOTV7N{Wy
MDA-MB-231  MmnXRZBweHSxc3nzJGF{e2G7 NV\UTGxzOi53LUeuOe69VQ>? NXLaOnZVOmh? NVLEOoNicW6qaXLpeJMhfGinIHPlcIwh\GWjdHigc4YhVUSDLV3CMVI{OSClZXzsd{BqdmS3Y3XkJIJ6KFOGVDDpckBiKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70JI1idm6nch?= M3HnOlIzOTF3NUK2
LNCaP NEW2[o5CeG:ydH;zbZMhSXO|YYm= NXTnW5BjPDEQvF2= Mn3FNog> NYPEbIc4cW6qaXLpeJMh[nW2ZXnuJIlv\HWlZXSgZ4VtdCCjcH;weI9{cXN? NWq4O2NPOjJzMUS3OlQ>
MB231 NVTHWmk4SXCxcITvd4l{KEG|c3H5 MXuxNFDPxE1? MX6xbC=> MmHISG1UVw>? NV7JOGdP[WK{b3fheIV{KHSqZTDpcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCkeTDXSWUyKGmwaHnibZRqd25uIGTSRWlNKHS{ZXH0cYVvfCxiYX7kJJRp\SClb33ibY5ifGmxbh?= NV76bJNQOjJzMUK5OFA>
HCC38  NITFTItCeG:ydH;zbZMhSXO|YYm= MX[xNFDPxE1? MkTKNYg> NGfXSIRFVVOR NULSU2hy[WK{b3fheIV{KHSqZTDpcoR2[3Srb36gc4Yh[2WubDDk[YF1cCCkeTDXSWUyKGmwaHnibZRqd25uIGTSRWlNKHS{ZXH0cYVvfCxiYX7kJJRp\SClb33ibY5ifGmxbh?= MWCyNlEyOjl2MB?=
MDA-MB231 MlvvRZBweHSxc3nzJGF{e2G7 MVq1NOKh|ryP NY\XVI97OjSq MofNSG1UVw>? NV7hRYZC[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MWSyNlExOTN|NR?=
LNCaP M1;5cWFxd3C2b4Ppd{BCe3OjeR?= MWK1NOKh|ryP NH;sUWgzPGh? MoDXSG1UVw>? MkjqZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MVOyNlExOTN|NR?=
HCT116 NWLWeHNDSXCxcITvd4l{KEG|c3H5 MV61NOKh|ryP NHvsUJYzPGh? MWTEUXNQ NX:3[ZN1[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MW[yNlExOTN|NR?=
Ishikawa  NHnOeHNCeG:ydH;zbZMhSXO|YYm= NHfnfoQzPc7:TR?= MlvJNlRp MnPxdoVlfWOnczDj[YxtKGSnYYToJIFv\CCjcH;weI9{cXNiaX7keYNm\CCkeTDCZYYhSTF? M{LWcVIzODh6OUG4
 YD-8  NEXDdXhCeG:ydH;zbZMhSXO|YYm= NELiRWoyODEQvF2= MmfUNYg> NE\X[GlqdmirYnn0d{BRVEWRLXnu[JVk\WRiYYDvdJRwe2m| NIXB[HczOjB6NkG4Ny=>
eosinophil  MkXURZBweHSxc3nzJGF{e2G7 M{H4VVkx|ryP M4LhVVI1cA>? NYPsRY94cW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliRnHzJIFvfGmkb3T5xsA> MUOyNlA4QTN|NB?=
L929 Ml\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIq1fY0zNjYQvF2= NXXMUWxzOjSq M{O2NIlvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJAueDN6IHHu[EBPTi4QulKgeI8h[XWpbXXueEBVVkcQsT3pcoR2[2WmIH7lZ5JweHSxc3nzJIFv\CCjdYTvdIhi\3l? NGm3SVEzOjB{N{C5Oy=>
YD-8  NEnvUm9CeG:ydH;zbZMhSXO|YYm= Mm\oNVAx|ryP NWG1Om1LOWh? MXjicI9kc3NidHjlJGdUNUiFbD3pcoR2[2WmIHHwc5B1d3Orcx?= MUOyNlAzODB5OB?=
HBx NUHBUJJ{SXCxcITvd4l{KEG|c3H5 Mo\sNlXPxE1? M4TLe|Q5cA>? NH;FRXBFVVOR M1;wSZJm\HWlZXSgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDNvTVG= MnLrNlIxOjByN{i=
U937  NUiwSZlqSXCxcITvd4l{KEG|c3H5 M3PhPFUxyqEQvF2= MoPXNYg> M4XnSIlvcGmkaYTzJGhHNWmwZIXj[YQh[XCxcITvd4l{yqB? MnnoNlE6QTh5M{G=
HL60 M{DPcmFxd3C2b4Ppd{BCe3OjeR?= MkL2OFDPxE1? MXW0NI1qdg>? MXHEUXNQ Mo\lZoxw[2u|IFLOSGMh[2:vcH;1coR{KGmwZIXj[UBmgHCxc4Xy[UBw\iCyaH;zdIhifGmmeXzz[ZJqdmViYX7kJGRPSSCocnHncYVvfGG2aX;u MWGyNVk5OzJ7Nh?=
M-14 Ml\DRZBweHSxc3nzJGF{e2G7 M2\4clI2|ryP Mo\UNE42cA>? MWpCpIlvcGmkaYTzJIJwfGhidHjlJINzfWSnIHX4eJJi[3RvIHHu[EBkd22yb4Xu[E1qdmS3Y3XkJIFxd3C2b4Ppdy=> NHnuTWgzOTl3NEm1PS=>
SK-BR-3 M1nIUmFxd3C2b4Ppd{BCe3OjeR?= NGjUfIk2OMLizszN M{GxRVJp NG\CZpJjdG:la4OgZZBweHSxdHnjJGRPSSCocnHncYVvfGG2aX;uJIlv\HWlZXSgZpkh[2:vYnnuZZRqd25ib3[gV20uOTZ2IHHu[EBz[WSrYYTpc44> NUPmbZBzOjF7MEGzPFY>
MDA-MB-468 M4j6SGFxd3C2b4Ppd{BCe3OjeR?= Mkf6OVDDqM7:TR?= MVWybC=> M2j3eoJtd2OtczDhdI9xfG:2aXOgSG5CKG[{YXft[Y51[XSrb36gbY5lfWOnZDDifUBkd22kaX7heIlwdiCxZjDTUU0yPjRiYX7kJJJi\GmjdHnvci=> NEDhNZYzOTlyMUO4Oi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3; 

PubMed: 27191889     


With the addition of Z-VAD-FMK, levels of cleaved Caspase-3 and PARP1 were decreased.

Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II; 

PubMed: 27191889     


Levels of Beclin1, Atg5, Atg7 and LC3-II were decreased after IFN-γ stimulation. However, when IFN-γ was combined with the caspase inhibitor, levels of Beclin1, Atg5, Atg7 and LC3-II were not decreased as determined by the Western Blot test. 

p23 / Hsp90 / hTRET / MMP2; 

PubMed: 19740745     


Expression of Hsp90 client proteins during cell death. HeLa cells were preincubated with z-VAD-fmk (100 μM) for 1 h and exposed to various concentrations of tunicamycin (Tu, in μM) for 24 h. Cell extracts were prepared and analyzed by Western blotting. β-Actin was used as the loading control.

cyto C; 

PubMed: 17301139     


z-VAD-fmk did not inhibit cytochrome c release in RRV-infected MA104 cells (RRV: rhesus rotavirus). Mock- and RRV-infected MA104 cells either were not pretreated or were pretreated with 100 μM z-VAD-fmk for 2 h before RRV infection, and treatment was continued during infection. Fourteen hours after RRV infection, the cytosolic and heavy-membrane fractions were assayed for cytochrome c by Western blotting. Mock-infected MA104 cells were used as negative controls. Cox IV and tubulin were used as protein loading controls for the heavy-membrane and cytosolic fractions, respectively. Positions of molecular weight markers are indicated on the left.

27191889 19740745 17301139
Growth inhibition assay
Cell viability; 

PubMed: 12522090     


Inhibition of magnolol-induced apoptotic cell death by z-VAD-fmk. CH27 cells were treated with cell permeable broad-spectrum caspase inhibitor (z-VAD-fmk) 2 h prior to 80 μm magnolol treatment. Cell viability was estimated. ***P<0.001, compared with the 80 μM magnolol-treated group. 

12522090
Immunofluorescence
cytochrome c ; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Smac/DIABLO; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Endo G; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of EndoG together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

AIF; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of AIF together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

11726499 12941691
ELISA
IL-1β; 

PubMed: 26254306     


Cell-free medium supernatants were harvested at 5 h. IL-1β levels were measured by ELISA. Z-VAD-FMK was used as a positive control. Bars represent means ± sem of pooled results from 3 experiments performed in triplicate at different times.

26254306
In vivo In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo[7].

Protocol

Cell Research:

[6]

+ Expand
  • Cell lines: human granulosa cell lines (GC1a, HGL5, COV434)
  • Concentrations: 50 μM
  • Incubation Time: 48 h
  • Method:

    To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h with etoposide (50 μg/ml) and/or Z-VAD-FMK (50 μM) under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses.


    (Only for Reference)
Animal Research:

[7]

+ Expand
  • Animal Models: CD1 mice
  • Formulation: dissolved in DMSO, diluted in PBS (final concentration of DMSO < 1%)
  • Dosages: 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (198.93 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+35 %PEG 300+2%Tween 80+ddH2O
For best results, use promptly after mixing.
6mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Can you suggest an in vivo formulation of S7023 for animal studies?

  • Answer:

    For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

  • Question 2:

    What is difference between S7023 & S8102?

  • Answer:

    Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

Caspase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID