Z-VAD-FMK

For research use only.

Catalog No.S7023 Synonyms: Z-VAD(OMe)-FMK

713 publications

Z-VAD-FMK Chemical Structure

CAS No. 187389-52-2

Z-VAD-FMK (Z-VAD(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

Selleck's Z-VAD-FMK has been cited by 713 publications

Purity & Quality Control

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Biological Activity

Description Z-VAD-FMK (Z-VAD(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Features A key compound for apoptosis studies.
Targets
Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro

Z-VAD-FMK (10 μM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 μM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U937 M4H4UmFxd3C2b4Ppd{BCe3OjeR?= M3zTN|Ux|ryP MoTVOFhp Ml\SdoVlfWOnczDj[YxtKGSnYYToJINifXOnZDDifUBUU0lvSVmgeJJm[XSvZX70xsA> NYr3bW5nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlQxPDNpPkK1PFI1ODR|PD;hQi=>
HL-60 MoXGRZBweHSxc3nzJGF{e2G7 NFPaOY82OM7:TR?= MX20PIg> MVfy[YR2[2W|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JHNMUS2LSTD0doVifG2nboVCpC=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NEC0N{c,OjV6MkSwOFM9N2F-
DTK-SME NHHhN2JCeG:ydH;zbZMhSXO|YYm= M134clUx|ryP M3LHTFJp NGj5O3ZFVVOR NUHyOIZveGG{dHnhcIx6KGmwaHnibZR{KHSqZTDhdI9xfG:|aYOgbY5lfWOnZDDifUBjfXCrdnHjZYlv\Q>? NHPkUI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi0N|g6Pyd-MkW4OFM5QTd:L3G+
HCT116  MYXBdI9xfG:|aYOgRZN{[Xl? MmG1OVDPxE1? NGC5dHIzcA>? MoPMbY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDESHZR NHrSVIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2PFgyQCd-MkW4Olg5OTh:L3G+
SGN NWPTWm5MSXCxcITvd4l{KEG|c3H5 MYeyNI1O NHTBfVk1QGh? M1\rUIhieyCwbzDpcoZtfWWwY3Wgc44hSUmILDDjZYxx[WmwIHX4dJJme3Orb36gc5Ih[2WubDDhdI9xfG:|aYO= NVThSW5sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4O|Q3OzNpPkK1PFc1PjN|PD;hQi=>
A549/V16 Mn;tRZBweHSxc3nzJGF{e2G7 M1zzRlUx|ryP NUG5SI1NOWh? M13nN5JmeHKnc4Pld{BVTy:WTT3pcoR2[2WmIHHwc5B1d3Orcx?= M{DmV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUS2NFM{Lz5{NUm0OlA{OzxxYU6=
U1 MkTxT4lv[XOnIFHzd4F6 M1fyWlExOM7:TR?= NUDTb|E5Omh? M4m5eolvcGmkaYTzJINie3Cjc3WtNy=> MkXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEC5OFIoRjJ3OUiwPVQzRC:jPh?=
ACH-2 MoXLRZBweHSxc3nzJGF{e2G7 MkjyNE0zODEQvF2= MXyybC=> NIf0e2Vz\WS3Y3XzJIRzfWdvaX7keYNm\CCjcH;weI9{cXNiYX7kJJN2[nOncYXlcpQhUEmYLUGgdoVxdGmlYYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MlPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEC5OFIoRjJ3OUiwPVQzRC:jPh?=
U1 NFP4XVdCeG:ydH;zbZMhSXO|YYm= NGqx[4YxNTFyMN88US=> NUn3TZAyOmh? MoLVdoVlfWOnczDkdpVoNWmwZIXj[YQh[XCxcITvd4l{KGGwZDDzeYJ{\XG3ZX70JGhKXi1zIILldIxq[2G2aX;uJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M3y3TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUiwPVQzLz5{NUm4NFk1OjxxYU6=
HUVEC-2c NXzBN2tHSXCxcITvd4l{KEG|c3H5 NFTwRpA2OM7:TR?= NWnSc49MPmh? NUPDSVBq\GWlcnXhd4VlKHSqZTDvfE1NTExvaX7keYNm\CCjdYTvdIhi\3l? NXPhdVlnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNlE4OjlpPkK2NFIyPzJ7PD;hQi=>
SP2/0 MlHFRZBweHSxc3nzJGF{e2G7 NHK2XlgyODEQvF2= NVnTV5JOOWh? M{HpUmROW09? M2DPTIJtd2OtczD0bIUh[XCxcITvd4l{KG:oIGPQNk8xKGOnbHzz NYfOTXNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wO|Q4OzJpPkK2NFc1PzN{PD;hQi=>
CEF M3;LTmtqdmG|ZTDBd5NigQ>? M1r3fVAtOzNuNkesNVAx|ryP NX6wb2hrOTWvaX6= MX7kc5dvNXKnZ4XsZZRmeyCSUjDlcpp6dWViYXP0bZZqfHlidH:gOFDwxIViYYSgNVAx|ryP NULhXYNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xNFI{OzlpPkK2NVAzOzN7PD;hQi=>
BEL-7402 M3XqUGFxd3C2b4Ppd{BCe3OjeR?= MmXVNlDPxE1? Mn\KNYg> MYXheJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMg M1n6XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MU[0O|k2Lz5{NkG2OFc6PTxxYU6=
HepG2 NHr3dINCeG:ydH;zbZMhSXO|YYm= Mki4NlDPxE1? MmnkNYg> MX\heJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMg Mlf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkS3PVUoRjJ4MU[0O|k2RC:jPh?=
HGL5 MnPNRZBweHSxc3nzJGF{e2G7 M3\PUlUx|ryP MX[yOIg> MUjpcoRq[2G2ZYOgZUBxem:2ZXP0bZZmKGWoZnXjeEBi\2GrboP0JIV1d3Cxc3nk[S=> Mn[1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkmwO|UoRjJ4MU[5NFc2RC:jPh?=
GC1a NVrhVVZNSXCxcITvd4l{KEG|c3H5 M{jCUVUx|ryP NXyzXolyOjSq MV;pcoRq[2G2ZYOgZUBxem:2ZXP0bZZmKGWoZnXjeEBi\2GrboP0JIV1d3Cxc3nk[S=> M3jyS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MU[5NFc2Lz5{NkG2PVA4PTxxYU6=
A549 NXLyRYFCSXCxcITvd4l{KEG|c3H5 MnzJNlAh|ryP NGHIWG4zPGh? NW\zZog1emWmdXPld{Bk\WyuIHTlZZRpKGOjdYPl[EBjgSCKRlPQJJRz\WG2bXXueC=> M1GzSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{m0NVQ6Lz5{NUe5OFE1QTxxYU6=
HeLa NIPsNVRCeG:ydH;zbZMhSXO|YYm= M3TXN|Ex|ryP NG\jcWk1QGh? NID0UYdl\WOuaX7ld{B1cGVicnH0[UBw\iCjcH;weI9{cXNiZILhcYF1cWOjbHz5 NXf3PWJ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O|I2PDVpPkK1O|czPTR3PD;hQi=>
SGC-7901 M1HZdWFxd3C2b4Ppd{BCe3OjeR?= NXrxW4tpOjEEoN88US=> MmnZNYg> NWTmenNCcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigbY5lfWOnZDDifUBwgGGuaYDsZZRqdg>? M2O3WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{[3NFc3Lz5{NUe2O|A4PjxxYU6=
MCF-7 NH35fVNCeG:ydH;zbZMhSXO|YYm= NIi3[W8yOM7:TR?= M3rUZ|Jp M1TuTIlvcGmkaYTzJJRp\SClZXzsJIRm[XSqIHnu[JVk\WRiYomgN2I> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd{MkGxOEc,OjV5MkKxNVQ9N2F-
A549 MVrBdI9xfG:|aYOgRZN{[Xl? MUexNEDPxE1? NYfT[lRXOjSq MWPEUXNQ M{\jZZBzd22xdHXzJGhDSy22cnXheIVlKEF3NEmgZ4VtdCC|dYL2bZZidCBoIHH0eIVvfWG2ZYOgeIhmKGOuZXH2[YQhWEGUUDDlfJBz\XO|aX;u NHO4WoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4N|U3QCd-MkW2PFM2Pjh:L3G+
U87 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{L3PFUx|ryP Mnv2NYg> NEX4NHJz\WOxdnXyd{Bk\WyuIHfyc5d1cCCocn;tJHROYiC2cnXheI1mdnR? MkC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEG2OlgoRjJ3NkixOlY5RC:jPh?=
Neocortical Neuron MoP3UoV2em:2b4jpZ4l1gSCDc4PhfS=> MWGxNFDPxE1? M3rUS|Fp MlfLZY51[Wexbnn6[ZMhUG:rYX3p[IUhSS2LbnT1Z4VlKE6ndYLveI95cWOrdIm= M{HNblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nke1NFAyLz5{NU[3OVAxOTxxYU6=
Primary human placental cytotrophoblasts NF30UI5CeG:ydH;zbZMhSXO|YYm= MnTxN|DPxE1? NVK2V4RbOjSq NV;ZVHRtTE2VTx?= MoDKdoV3\XK|ZYOgeIhmKGmwaHnibZRqd25ib3[gNVHPui2KU1SyJIJ6KHS{aXPsc5NidsLi M2f0U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkSyOVkzLz5{NU[0NlU6OjxxYU6=
MM Ml3iRZBweHSxc3nzJGF{e2G7 NGXmZ2c2OM7:TR?= MoG1NlBucW5? NHfIZlZx[XK2bImgbY5pcWKrdIOgV2hMNWmwZIXj[YQhKGOnbHyg[IVifGh? MoTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{CwPVgoRjJ3NUOwNFk5RC:jPh?=
DLD1 NFTY[mtCeG:ydH;zbZMhSXO|YYm= NVnmdJpLOjEEoN88US=> NU[1RmZFOWh? NF;BVIVx[XK2bImgdoV3\XK|ZYOgZ4VtdCCjcH;weI9{cXNiY3H1d4VlKGK7IGfTVFE> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV{NEK0Okc,OjV3MkSyOFY9N2F-
MDA-MB-231 MoHNR5l1d3SxeHnjbZR6KEG|c3H5 NIXlVokyOM7:TR?= MV:xbC=> MkL2ZZVodWWwdIOgZ4VtdCCmZXH0bEBi\nSncjDDTHVCKHS{ZXH0cYVvfA>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV{MUWwNUc,OjV3MkG1NFE9N2F-
MKN28 MXXBdI9xfG:|aYOgRZN{[Xl? MYmxNO69VQ>? NUHublFHOzCvaX6= M3npeGROW09? M1PGTIlvcGmkaYTzJHRPTi4QsTDwcJV{KEOKWD3pcoR2[2WmIHHwc5B1d3Orcx?= NF3nVGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWxN|k3OCd-MkW1NVM6PjB:L3G+
HL-60 NFjkNnlEgXSxdH;4bYNqfHliQYPzZZk> NILXd2IyODEQvF2= M1HqN|Fp NULPVIozTE2VTx?= Mn;nxsBqdXC{b4\ld{B3cWGkaXzpeJkhd2ZiVFPORZMufHKnYYTl[EBk\Wyucx?= NUPGVZRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NFI6OzJpPkK1OVAzQTN{PD;hQi=>
AGS MVjBdI9xfG:|aYOgRZN{[Xl? MlfkNVDPxE1? NXXV[G9xOWh? NH\6PHZxemW4ZX70d{BkfXKldX3pck1qdmS3Y3XkJINt\WG4YXflJI9nKGOjc4Dhd4UuOyxiLUisJIFv\CBvOTDwdo91\Wmwcx?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7MkKxOEc,OjV2OUKyNVQ9N2F-
A549  Mo\PRZBweHSxc3nzJGF{e2G7 NGrZUlcyOM7:TR?= NFu3PGYzPGh? M4C4N4Jtd2OtczDEUWFUNWmwZIXj[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHHu[EBieG:ydH;0bYMh[2WubDDk[YF1cA>? NUT4foY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QDlpPkK1OFM1QTh7PD;hQi=>
INS-1 M{DjR2Fxd3C2b4Ppd{BCe3OjeR?= M1j3TVUx|ryP NWDWUWF3Pmh? NXTadWFlTE2VTx?= MWjk[YNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[g[YFzdHliYYDvdJRwfGmlIHPlcIx{yqB? NFHITJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzNFg6Pyd-MkW0N|A5QTd:L3G+
Primary hepatocytes  MYHBdI9xfG:|aYOgRZN{[Xl? NGr4fFI2OM7:TR?= NWnQRYV{OTiq NWnFRpVXcW6qaXLpeJMh[XCxcITvd4l{KG:oIHjldIF1d2O7dHXzJIlv\HWlZXSgZpkhSWO2IFSgZY5lKFSQRj5OtS=> M3fGXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEC3OVM5Lz5{NUSwO|U{QDxxYU6=
L929-A  MXfDfZRwfG:6aXPpeJkhSXO|YYm= NUjqcYoyOjEEoN88US=> NEjUWlczPGh? NVP5N5JvcW6qaXLpeJMhXE6IzsGtbY5lfWOnZDDj[YxtKGSnYYTo M1TidVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{m4OVQxLz5{NUO5PFU1ODxxYU6=
L929-N MYrDfZRwfG:6aXPpeJkhSXO|YYm= NGTNW|EzOMLizszN NWrwcGZCOjSq MnP0[Y5p[W6lZYOg[IVifGhidnnhJIF2fG:lcnnu[UBVVkcQsTDwdo9lfWO2aX;u NYHNTmhPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzPVg2PDBpPkK1N|k5PTRyPD;hQi=>
Cytotoxicity Assay MVrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVyyNO69VQ>? NVq4cnloPDiq MVLwdoV3\W62IF3IUWQucW6mdXPl[EBk\WyuIHTlZZRp MmS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OUKxNVYoRjJ3M{myNVE3RC:jPh?=
COS7 MXvLbY5ie2ViQYPzZZk> NXnkW3U4OTEQvF2= NULJRWMxOjSq MnnkbY5kemWjc3XzJINie3Cjc3WgN{84KGGldHn2bZRq\XN? NH;2cmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO4OFAzPSd-MkWzPFQxOjV:L3G+
COS7 NFe4cJpCeG:ydH;zbZMhSXO|YYm= NUjSU4lGOTEQvF2= M1XYeFQ5cA>? NXnoNHNqeGG{dHnhcIx6KHC{ZY\lcpRm\CCIQ{GwNU1qdmS3Y3XkJINmdGxiZHXheIg> Mlr4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OESwNlUoRjJ3M{i0NFI2RC:jPh?=
A375 MmnTRZBweHSxc3nzJGF{e2G7 M3fJ[lMx|ryP NI\6eXYzcA>? M1rXUpBz\X[nboTzJJRp\SCmcoXnMYlv\HWlZXSgVGFTWCClbHXheoFo\Q>? Ml;QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|N{[xNVUoRjJ3M{e2NVE2RC:jPh?=
A549 MofwRZBweHSxc3nzJGF{e2G7 MVi1NO69VQ>? NIfEZ|gzPGh? Mo\PdoV3\XK|ZYOgdoljd3OxbXWgZolw\2WwZYPpd{BidmRiYYDvdJRwe2m|IHPheZNm\CCkeTCgR4hqcm:wZ3Thci=> M3SzTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{S5O|gyLz5{NUO0PVc5OTxxYU6=
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A375 MUXBdI9xfG:|aYOgRZN{[Xl? NFL4UY8zOM7:TR?= MYiyOIg> MnOzdoV3\XK|ZYOg[IlkcXS{aX7vcoUhSi2rbnT1Z4VlKGGyb4D0c5Nqew>? MnPjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OUmxNVEoRjJ2Nkm5NVEyRC:jPh?=
HDPC NWL1WpE3SXCxcITvd4l{KEG|c3H5 MnXpOVDDvU1? MkHvNlRp MVvpcohq[mm2czDOU{1qdmS3Y3XkJIFxd3C2b4Ppdy=> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|NEW5N{c,OjR4M{S1PVM9N2F-
A549 M1myR2Fxd3C2b4Ppd{BCe3OjeR?= M4fIdVUxyrWP NHqyV5AzcA>? MlzESG1UVw>? Mo[0dJJmfmWwdIOgeIhmKGi7cH;kbZBtd2mmIFTORUBkd262ZX70JJBp[XOnIHnu[JVk\WRiYomgZ4VxcGGub3Podo9ucW5? NEK3bXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW4PFE{PSd-MkS1PFgyOzV:L3G+
 BV-2 MV3BdI9xfG:|aYOgRZN{[Xl? MWOyNO69VQ>? M3\ZXlJp M1HCUJN2eHC{ZYPz[ZMhXVZvaX7keYNm\CClaILvcYF1cW5iZYj0dpV{cW:wIHHu[EBlcWyjdHnvckBw\iC2aHWgcpVkdGWjcjDlcpZmdG:yZR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV3OEGxPEc,OjR3NUixNVg9N2F-
HT-29  MXTBdI9xfG:|aYOgRZN{[Xl? MmnJOVDPxE1? MkG0OFhp NHrDO5pjdG:la4OgeIhmKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IHvh[Y1x\mW{b3y= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV2OUG3OUc,OjR3NEmxO|U9N2F-
A549 NHjkZWVCeG:ydH;zbZMhSXO|YYm= M4PNR|XPxE1? NYP5TWQxOjSq NIHNVVd{fXCycnXzd4V{KD[nwrCoRmsyODB2MDmtbY5lfWOnZDDhdI9xfG:|aYRCpC=> NYOxcpN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1Nlk5PzFpPkK0OVI6QDdzPD;hQi=>
MIA-PaCa-2 M37UXWFxd3C2b4Ppd{BCe3OjeR?= NYTHdXo4OjSq NUnpOG57[myxY3vzJINt\WG4YXflJI9nKGOjc4Dhd4UuOyCrbnT1Z4VlKGK7IHLveIghSS12NEO2OVQh[W6mIIDhZ4xqfGG6ZXy= M1LUcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUGwPVkzLz5{NEWxNFk6OjxxYU6=
Caki-1 NGDIVoFCeG:ydH;zbZMhSXO|YYm= M4HyWlIx|ryP M2fLc|Fp MV7pcohq[mm2czDTR3AucW6mdXPl[EBieG:ydH;zbZM> NFrGe4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwOFY5OSd-MkS1NFQ3QDF:L3G+
CLL MoDwRZBweHSxc3nzJGF{e2G7 MYOyOe69VQ>? NHTJ[nQycA>? MXfwZZJ1cWGubImgZoxw[2u|IF3MUlIzOzhvaX7keYNm\CClZXzsJIFxd3C2b4Ppdy=> MoXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2Nke2N|QoRjJ2NE[3OlM1RC:jPh?=
SGC-7901 MnPCRZBweHSxc3nzJGF{e2G7 NVfBPXduOTEQvF2= Moq1NlRp M4DScZBzd22xdHXzJJRp\SCFR1nJMYlvcGmkaYTl[EBk\WyuIHfyc5d1cCCrbjDTS2MuPzlyMTDj[Yxtew>? M2jiZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEW0OFg5Lz5{NES1OFQ5QDxxYU6=
k1735 MojJRZBweHSxc3nzJGF{e2G7 MUOyNQKBkc7:bR?= MY[0bC=> NFvoS|JqdmirYnn0d:KhW2GubX;u[Yxt[S2rbnT1Z4VlKGGyb4D0c5Nqew>? MnjWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUGxNVYoRjJ2NEWxNVE3RC:jPh?=
HaCaT  Ml7hRZBweHSxc3nzJGF{e2G7 MW[xNFDPxE1? NFLzWIMycA>? NX;wbndU[my3boTzJHVXSi2rbnT1Z4VlKGGyb4D0c5NqeyCrbjDIZWNiXCClZXzsdy=> NX\2fWFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVY6QTdpPkK0N|U3QTl5PD;hQi=>
podocytes NX\aRnNTSXCxcITvd4l{KEG|c3H5 MkLnNlAx|ryP M1PlS|Zp NGLT[pdqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSB|LEStSGdG NXrZSm5YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzN|c4PzdpPkK0N|M4Pzd5PD;hQi=>
 KB M1nZTWFxd3C2b4Ppd{BCe3OjeR?= NUTQT5R1PTEQvF2= M{jnZVI1cA>? MWHpcohq[mm2czDMbYNwNUFvaX7keYNm\CClYYPwZZNmNTNiYX7kJHBCWlBiYXP0bZZifGmxbh?= Mnu5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|M{e0PVIoRjJ2M{O3OFkzRC:jPh?=
 HepG2  MnXhRZBweHSxc3nzJGF{e2G7 MXWxNO69VQ>? NGOwZWMzPGh? MXLy[YR2[2W|II\pcYVvfGmwIHPs[YF3[WenIHPheZNm\CCkeTDMVHM> NHPQbI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyOVgyPid-MkSzNlU5OTZ:L3G+
fetal rat lung fibroblasts  M1HO[mtqdmG|ZTDBd5NigQ>? NIP5OI05OM7:TdMg MlHiNYg> NXjQZpRqcW6qaXLpeJMhS2G|cHHz[U0{KGGldHn2bZR6yqB? M4fzTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G0NVM2Lz5{NEOxOFE{PTxxYU6=
PDL fibroblasts MmXCT4lv[XOnIFHzd4F6 MXS4NO69VcLi NEDiUGIycA>? Mn3SbY5pcWKrdIOgR4F{eGG|ZT2zJIFkfGm4aYT5xsA> M{D3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G0NVM2Lz5{NEOxOFE{PTxxYU6=
MB49 NWXEbopHSXCxcITvd4l{KEG|c3H5 MXGyNO69VQ>? MVmxbC=> MknHdoV3\XK|ZYOgR20ucW6mdXPl[EBk\WyuIHTlZZRp NID0bIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK4NlQ{Oyd-MkSyPFI1OzN:L3G+
5637 NGnDNWNCeG:ydH;zbZMhSXO|YYm= Mny4NlDPxE1? MWSxbC=> MVPy[ZZmenOnczDDUU1qdmS3Y3XkJINmdGxiZHXheIg> NWrlZmVnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFI1OzNpPkK0NlgzPDN|PD;hQi=>
A549  NXLoenlZSXCxcITvd4l{KEG|c3H5 NH7iNmUyODEQvF2= NVzRXVg1PDiq M{PtWJN2eHC{ZYPz[ZMhfGinIHHwc5B1d3OrczDjZZV{\WRiYomgdIlx\XKrbnZCpC=> M{XMTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkeyNlAyLz5{NEK3NlIxOTxxYU6=
MCC-2 MlrrRZBweHSxc3nzJGF{e2G7 NUnK[oJzOjBizszN MoH6Oog> MXTEUXNQ NG\6b|RjdG:la4OgbY5lfWOnZDDifUB{fGG3cn;zdI9zcW6nIHHwc5B1d3OrczDj[YxtKGSnYYTo MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4Mk[1PEc,OjR{NkK2OVg9N2F-
ADF M4DqeWFxd3C2b4Ppd{BCe3OjeR?= MlXEOFDPxE1? MYK2bC=> NFrsb|NqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClaHXtc5Rp\XKjcImgdIx2eyCcblPsNi=> MlnFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
 U373  M3rCPGFxd3C2b4Ppd{BCe3OjeR?= MnzSOFDPxE1? MoHiOog> MV\pcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBkcGWvb4To[ZJieHlicHz1d{BbdkOuMh?= MkGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
RKO-HIPK2i MYLBdI9xfG:|aYOgRZN{[Xl? MkjNOFDPxE1? NX3oOWdKPmh? NEnpXIpqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClaHXtc5Rp\XKjcImgdIx2eyCcblPsNi=> MorNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
HEC-1B MlKxRZBweHSxc3nzJGF{e2G7 NWPNPVNzOjEQvF2= NX;pUHNxOWh? MV\y[YR2[2W|IGTQMYlv\HWlZXSgZZBweHSxc3nzxsBk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOR?= MnfzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUOzOVgoRjJ2MkGzN|U5RC:jPh?=
4T1 NXnsTYVPS2WubDDWbYFjcWyrdImgRZN{[Xl? Ml6wNk42NTFyzszN M2XyR|Rp M{XBWWROW09? MXvy[ZNkfWW|IITo[UBkgXSxdH;4bYNqfHlib3[gOHQyKGOnbHzzJIlv\HWlZXSgZpkhW1CGVDDpckBiKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70JI1idm6nch?= NIrLWG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwOlE3OSd-MkSyNFYyPjF:L3G+
OS  MoXMRZBweHSxc3nzJGF{e2G7 NV;oTI93OjEQvF2= NYXoZYFQPGh? MV;pcohq[mm2czD0bIUh[2WubDDk[YF1cCClYYXz[YQh[nliUFTU NX3rWZdnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFQ6OzdpPkK0NlA1QTN5PD;hQi=>
RPE NEDQUnRCeG:ydH;zbZMhSXO|YYm= M{m4UVExOM7:TR?= M4HM[VQ5cA>? NVLWfHpmeGG{dHnhcIx6KGmwaHnibZR{KHSqZTDjZYxx[WmwLUGgZY5lKC1{IHHjeIl3[XSrb36gZZMhf2WubDDhd{B1cGViY3HzdIF{\SCjY4TpeoF1cW:w MlOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MEKwOVIoRjJ2MkCyNFUzRC:jPh?=
UD29a Mm[4RZBweHSxc3nzJGF{e2G7 NHzjPHE2OM7:TR?= MmnBNlRp MWrpcohq[mm2czD0bIUh[2WubDDk[YF1cCClYYXz[YQh[nliTmXUNy=> Mne5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOUC1O|QoRjJ2MUmwOVc1RC:jPh?=
SH-SY5Y  MkfZRZBweHSxc3nzJGF{e2G7 MYexNO69VQ>? NGCyWngzPGh? MmjpZZR1\W63YYTld{Bk[XOyYYPlJIFkfGm4YYTpc44h[W6mIHPlcIwh\GWjdHigbY5lfWOnZDDifUBJVkVvMlTH MoT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNEW0OlMoRjJ2MUS1OFY{RC:jPh?=
HeLa  MlHIRZBweHSxc3nzJGF{e2G7 NXTublhbOTBxMkFOwG0> NWLZc2hTOjSq NHfnZ|BqdmirYnn0d{B1cGViYXP0bZZqfHlib3[geIhmKG2jan;ybZR6KG:oIITo[UBu\W2kZYLzJI9nKHSqZTDjZZNx[XOnLX\hcYltgQ>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|N{K2Okc,OjRzM{eyOlY9N2F-
SCCVII  MkHTRZBweHSxc3nzJGF{e2G7 Mk[yNlXPxE1? NIfsNmsycA>? MoW1bY5pcWKrdIOgeIhmKGOnbHygb4ltdGmwZzDh[pRmeiCmYYPheIlvcWJ? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF{NkS2OEc,OjRzMk[0OlQ9N2F-
RAW 264.7 Ml;HRZBweHSxc3nzJGF{e2G7 NX;jNHVnOjEQvF2= MVSxPIg> Mm\pbY5kemWjc3XzJGxEOy2LST1Otk1i[3SrbjDyZZRqdyClb33wZZJm\CC2bzDFR3RXNU2RUzD0doVifG2nboSgc45tgQ>? NInkSo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGxOlcxPyd-MkSxNVY4ODd:L3G+
A549  M1nWUGFxd3C2b4Ppd{BCe3OjeR?= M1LIVFIvPc7:TR?= Mne1NlRp M4j3[YRm[3KnYYPld{Bwemmmb37pck1qdmS3Y3XkJIF2fG:yaHHnfUBieyC5ZXzsJIFv\CCOb4PzJI9nKM7Wz5jtJIFte29ib3PjeZJz\WRiZIXybY5oKGG3dH;wbIFocWNicILvZ4V{ew>? MoG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEK1NlIoRjJ2MUCyOVIzRC:jPh?=
UM-SCC-10A  NF31[ZdCeG:ydH;zbZMhSXO|YYm= MX:1NOK2VQ>? MoHrNog> NH\LUodz\WS3Y3XzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGirZ3it[I9{\SCrc3;hcIFvfG:uYXP0c45m MnG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3OVMoRjJ2MEm4O|U{RC:jPh?=
BGC-823 NHzMfWJCeG:ydH;zbZMhSXO|YYm= MmX3NVAh|ryP NY\JUVdwOjSq NE\VcnVz\WS3Y3XzJJRmfHKjbnTybY5mNWmwZIXj[YQh[XCxcITvd4l{yqB? NV\Sb4JIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVg2OTFpPkK0NFk5PTFzPD;hQi=>
Nalm-6  MVjBdI9xfG:|aYOgRZN{[Xl? NVXxS|R4OjEQvF2= MVu0bC=> MmjSbY5pcWKrdIOgZ4F{eGG|ZT24JIFv\CClYYPwZZNmNTNiYXP0bZZifGmxbjDhcoQhWEGUUD2xJINt\WG4YXfl NUX4fmNFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwN|k6PjdpPkK0NFM6QTZ5PD;hQi=>
KHOS  NI\lZmxCeG:ydH;zbZMhSXO|YYm= M3r2TlQx|ryP MX2yOIg> NWjxTZNT[myxY3vzJJRp\SCrbnPy[YF{\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZT2zJIFv\CCSQWLQJIlv\HWlZXSgZpkhSkKPRERCpC=> MnTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMkWzOlEoRjJ2MEK1N|YyRC:jPh?=
G292 NWnaNG56SXCxcITvd4l{KEG|c3H5 MWK0NO69VQ>? M1fJRlI1cA>? NUT4dIs6[myxY3vzJJRp\SCrbnPy[YF{\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZT2zJIFv\CCSQWLQJIlv\HWlZXSgZpkhSkKPRERCpC=> M{m0NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK1N|YyLz5{NECyOVM3OTxxYU6=
MG-63 MmDnRZBweHSxc3nzJGF{e2G7 MXO0NO69VQ>? MnvQNlRp Mm[2Zoxw[2u|IITo[UBqdmO{ZXHz[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgRmJOTDQEoB?= NW\XV|JmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlU{PjFpPkK0NFI2OzZzPD;hQi=>
EBV-transformed B cells NFzrOHVCeG:ydH;zbZMhSXO|YYm= MVuyNO69VQ>? M2T2OVJp NH:wRWpFVVOR NVLmO4E2[myxY3vzJIFvfGlvQ1S4NEBidmRiYX70bU1ETDh4IHHueIljd2S7LXnu[JVk\WRiYYDvdJRwe2m| NE\NTpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECwPFYzQCd-MkSwNFg3Ojh:L3G+
IM-9 B NEPFcotCeG:ydH;zbZMhSXO|YYm= M3jSTFIx|ryP M1XGbFJp MULEUXNQ MoOxZoxw[2u|IHHueIkuS0R6MDDhcoQh[W62aT3DSFg3KGGwdHnic4R6NWmwZIXj[YQh[XCxcITvd4l{ MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDByOE[yPEc,OjRyMEi2Nlg9N2F-
CNE1 NHm4d3lCeG:ydH;zbZMhSXO|YYm= NYi5cXRwOjEQvF2= MWS0PIg> NWTKS5pU[myxY3vzJGxMNUFvaX7keYNm\CCjcH;weI9{cXN? NYXsfGRoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PFUxOjlpPkKzPVg2ODJ7PD;hQi=>
CNE2 NHP6T2lCeG:ydH;zbZMhSXO|YYm= M{HmWlIx|ryP MXO0PIg> MXPicI9kc3NiTFutRU1qdmS3Y3XkJIFxd3C2b4Ppdy=> NELTPJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m4OVAzQSd-MkO5PFUxOjl:L3G+
HL-60 MUjBdI9xfG:|aYOgRZN{[Xl? NXPwTllNPTEEtV2= M{jjUVFp NXX1c|ViTE2VTx?= M{PrW4lvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEKDMUS1 MlXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NEi3OVEoRjJ|OUS4O|UyRC:jPh?=
L929 NFH5eG1CeG:ydH;zbZMhSXO|YYm= M{HhT|EvOjYkgKO1xsDPxE4EoB?= MmnlNlRp NWS3Z2Mz[XWpbXXueJMhXE6IzsGtbY5lfWOnZDDu[YNzd3C2b4Ppd{BidmRiYYX0c5Bp[We7 NFni[nM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0NVc3QSd-MkO5OFE4Pjl:L3G+
Jurkat NEDtbYlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NVTScG9wOs7:TR?= MXi0bC=> NHXCb5Rz\X[ncoPld{Boem:5dHigbY5pcWKrdHnvckBidmRizsKtZ4F1\W6rbjDk[YNz\WG|ZTDifUBJWy2DU1G= M{DXNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEm2NFYyLz5{M{i5OlA3OTxxYU6=
RAW264.7  NY\Ob4pmSXCxcITvd4l{KEG|c3H5 Mn7ONVAh|ryP NVPCU|hWOjSq M3rUfoRm[3KnYYPld{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgS2E> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEKwN{c,OjN6MkCyNFM9N2F-
A431  NEK4THpMcW6jc3WgRZN{[Xl? NVLEPHlRPDEQvF2= MVSybC=> M17KVYJtd2OtczDmbZNmfGmwLXnu[JVk\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZYOgZY5lKFCDUmC= NETpNmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwNFA2QCd-MkO4NFAxPTh:L3G+
Jurkat NHvoUY1E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NX6y[W9mOjVvMUCw{txO NXzFd2FzPmh? NI[zd|RqdmirYnn0d{B7NU[DLVPNT{1qdmS3Y3XkJINmdGxiZHXheIg> NW[1bJM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5N|M2OzJpPkKzPVM{PTN{PD;hQi=>
COS-7  Mm\jSpVv[3Srb36gRZN{[Xl? NGrH[pA2OML3TR?= NXrtOZdvOjSq M{jiS4Fn\mWldIOgeIhmKHC{b3Pld5Nqdmdib3[gRXRPOXNid3n0bEBxd2y7UTD0doFkfHN? Mo[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{OyNFgoRjJ|OUOzNlA5RC:jPh?=
CAL27 NHjUOnJCeG:ydH;zbZMhSXO|YYm= Mnq5NVAh|ryP MYKybC=> M{PGd5N2eHC{ZYPz[ZMhS3W{LV7Qd{1z\WS3Y3XkJJZq[WKrbHn0fUBjgSC3cDD0c{A6OCV? MlrkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MUezPVYoRjJ|OUG3N|k3RC:jPh?=
THP-1  M4H5fWFxd3C2b4Ppd{BCe3OjeR?= MnrSNVAuPTEQvF2= MorWNog> MlTjbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhfHKrcITvcIll\Q>? NXT1Wpc4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFAzQTlpPkKzPVAxOjl7PD;hQi=>
K562 M4TsNGFxd3C2b4Ppd{BCe3OjeR?= M4nLOVIx|ryP M3HTTFQ5cA>? NGPQc3BqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSB2LV3V MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5NkiyOkc,OjN6N{[4NlY9N2F-
HL-60 MYHBdI9xfG:|aYOgRZN{[Xl? NHniZ28yODEQvF2= M2\ITFI1cA>? M3\Wb4lvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGGkaXX0ZY5mKGSrdHXydIVv\XQEoB?= NEG5RoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i2OVc4QCd-MkO4OlU4Pzh:L3G+
HeLa  MoL3RZBweHSxc3nzJGF{e2G7 MWiyNO69VQ>? M2j5VlJp MVjpcohq[mm2czDEUW1RKGmwZIXj[YQh[XCxcITvd4l{ MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4M{m2Okc,OjN6NkO5OlY9N2F-
U251 MnThRZBweHSxc3nzJGF{e2G7 NX\6SIhLOjEQvF2= MlXKNlRp NWD2eGNjcW6qaXLpeJMh[2G|dHnjbY4hcW6mdXPl[EBIOi:PIIDoZZNmKGG{cnXzeEBidmRiYYDvdJRwe2m| MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzNkixOkc,OjN6MU[4NVY9N2F-
HL-60 NESyUoFCeG:ydH;zbZMhSXO|YYm= NFewOWU2OML3TR?= NXXnc4dpPGh? NH7kXmtjdG:la4OgeIhmKGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzygMVktKGGwZDDQRXJRKGmwZIXj[YQh[nliNoO= MnPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MES3NFYoRjJ|OEC0O|A3RC:jPh?=
Ec-109 MnX2RZBweHSxc3nzJGF{e2G7 MVKxNEDPxE1? NIrCdGc1QGh? MX7y[ZBz\XO|ZYOgVGUudWWmaXH0[YQhTWNvMUC5JINmdGxiYYDvdJRwe2m| NX3nbnFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PFI3PDFpPkKzO|gzPjRzPD;hQi=>
RKO MmPiR4VtdCCYaXHibYxqfHliQYPzZZk> Mon5NVAh|ryP NYnhXIw6OjSq NU[0NYZEcW6qaXLpeJMhfGinIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7IHPheZNm\CCkeTDERWI> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd3OEC2OEc,OjN5NUiwOlQ9N2F-
T98G MnPJR4VtdCCYaXHibYxqfHliQYPzZZk> NF;RT4UyNTFyMN88US=> M2rOblI1cA>? NX\QRWg1cW2ycn;2[ZMh[2WubDD2bYFjcWyrdImgZ491emWjdH3lcpQhf2m2aDDUVy=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd4OUK3OUc,OjN5NkmyO|U9N2F-
Jurkat NHXHfFZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MXSxNFAuOjByzszN NWfSNnZVOjSq MoL2bY5pcWKrdIOgTIFCPCCrbnT1Z4V{KGGyb4D0c5NqeyCmZYDlcoRmdnRib3[gZ49v[2WwdILheIlwdsLi MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|MkS4NUc,OjN5M{K0PFE9N2F-
Molt-3  MlPQRZBweHSxc3nzJGF{e2G7 MWq1NO69VQ>? M{LZ[lJp MVHy[YR2[2W|IH3lcIF1d26rbj3pcoR2[2WmIHHwc5B1d3Orcx?= NWfhbllPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NlUxOTNpPkKzO|I2ODF|PD;hQi=>
hMSC12 NGjpb5hCeG:ydH;zbZMhSXO|YYm= NWDVcphPOTByzszN M{PR[FRl NUPIPIlIcW6qaXLpeJMhd3O2ZX;n[Y5q[yCldXz0eZJmNWmwZIXj[YQh[2WubDDk[YF1cCCjbnSgZ4Ft[2moaXPheIlwdg>? M3LUPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkW3PFIzLz5{M{[1O|gzOjxxYU6=
HM7  M2X1OGFxd3C2b4Ppd{BCe3OjeR?= MV2yNO69VcLi NWXMeYdFOWh? NFjIdIxjdG:la4OgZZBq[3WuYYLlckBCKGGlZYTheIUucW6mdXPl[EBk[XOyYYPlMVMh[WO2aY\heIlwdiCjbnSgVGFTWCClbHXheoFo\Q>? NG\jdYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W4N|QyOid-MkO1PFM1OTJ:L3G+
Hep-2  NX71SHpMSXCxcITvd4l{KEG|c3H5 MYeyNO69VcLi NUn1Xot5OjSq NHzIW2hFVVOR MkfBZYxt\X[rYYTld{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliaX7keYNm\CCkeTDzbYxq[mmwaX6= NFTLfoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W4NFA{Oid-MkO1PFAxOzJ:L3G+
HSCs Mn64RZBweHSxc3nzJGF{e2G7 M3fON|Ux|ryP NHLTcJgzPGh? NGj2XYVFVVOR MVzpcohq[mm2czDubYxwfGmwaXKtbY5lfWOnZDDEUmEh\GGvYXflJIlv\GmlYYTl[EBjgSCSQWLQJINt\WG4YXfl M3rCRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm5PFc1Lz5{M{S5PVg4PDxxYU6=
HL-60  M{TwNWN6fG:2b4jpZ4l1gSCDc4PhfS=> MYWyNO69VcLi M{W2d|I1cA>? NUO2XXJ6emWmdXPld{B1cGViY4n0c5RwgGmlIHXm[oVkfCCxZjDFdpk2 MojUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUS0PFAoRjJ|NEm0OFgxRC:jPh?=
HA NFHnR2RCeG:ydH;zbZMhSXO|YYm= Mn3rOVDPxE1? MVmyOIg> MkjBd5VxeHKnc4Pld{B1cGViY3zlZZZi\2Vib3[gVGFTWCCjbnSgZ4F{eGG|ZT2zMEAuPyxiYX7kJE06KGmwZIXj[YQh[nliYn;yeIV7d22rYj;TRWhC NXr5ZYpXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0O|U6PTZpPkKzOFc2QTV4PD;hQi=>
C666-1 NVjLfVZZSXCxcITvd4l{KEG|c3H5 NVO1b2tQPTEQvF2= MYGyOIg> MWLzeZBxemW|c3XzJJRp\SClbHXheoFo\SCxZjDQRXJRKGGwZDDjZZNx[XOnLUOsJE04NCCjbnSgMVkhcW6mdXPl[EBjgSCkb4L0[ZpwdWmkL2PBTGE> MoroQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{W5OVYoRjJ|NEe1PVU3RC:jPh?=
Hepa1-6 NXLtUFdiSXCxcITvd4l{KEG|c3H5 M{fSVVUx|ryP MWOybC=> M3rL[YlvcGmkaYTzJIN2emO3bXnuJIFv\CC{ZYP2[ZJifHKxbD3pcoR2[2WmIHHwc5B1d3Orcx?= NF3QU2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0Olc2Oyd-MkO0OFY4PTN:L3G+
PLC/PRF/5c NYjKR3g{SXCxcITvd4l{KEG|c3H5 M{ezWVUx|ryP MnH0NYg> M{TKfZBz\X[nboTzJIFxd3C2b4Ppd{B1emmpZ3Xy[YQh[nliQ1nO NGHLXHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SzPFgzPCd-MkO0N|g5OjR:L3G+
HCT116 NIrzW2NCeG:ydH;zbZMhSXO|YYm= Mn\5NVAx|ryP MmnzNU42cA>? MoOwbY5pcWKrdIOgU5MucW6mdXPl[EBk\WyuIHTlZZRp Moj0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NESzOVMoRjJ|N{S0N|U{RC:jPh?=
HCEC NW\DblZJTnWwY4Tpc44hSXO|YYm= M1;oelUx|ryP MVS3Nog> NGPJcJZFVVOR NF20cIlz\XO2b4Lld{Bw\iC2aHWgco9zdWGuIFjDSWMheGinbn;0fZBm NUfv[VN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OFIxOTFpPkKzO|QzODFzPD;hQi=>
Primary rat cerebral cortical neurons MlrzRZBweHSxc3nzJGF{e2G7 MkjsNVAx|ryP M1TuSFFp M1XEdZBz\X[nboTzJGNlNWmwZIXj[YQh[XCxcITvd4l{KGGwZDDj[YxtKGSnYYTo MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2MUOxO{c,OjN5NEGzNVc9N2F-
MDA-MB-231  MYnBdI9xfG:|aYOgRZN{[Xl? NXHRdYpkOjYQvF2= M2fzTFI1cA>? MWLhZpJw\2G2ZYOgZ5l1d3SxeHnjbZR6KGGwZDDjcIVifmGpZTDv[kBk[XOyYYPlMVMh[W6mIGDBVnAhcW6mdXPl[EBjgSCUZ{O= NYHtUFNtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzN|c2PDRpPkK1N|M4PTR2PD;hQi=>
NLRP3-Tet-on-MC/9  NXzud|M5SXCxcITvd4l{KEG|c3H5 NVvQR|BDOTBvNEFOwG0> NIL3OFMyOmh? NXW5OFhV[XS2ZX71ZZRmeyC2aHWgZ4VtdCCmZXH0bEBk[XW|ZXSgZpkhS0GSUz3hd5Nw[2mjdHXkJG5NWlB|IH31eIFvfHNiaX6geIhmKFSndD3vckB{gXO2ZX2= MlL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEOzPFkoRjJ|N{CzN|g6RC:jPh?=
KNS42  NEXobXJCeG:ydH;zbZMhSXO|YYm= M131cFUx|ryP MmnZNYg> MUHy[YR2[2W|IHPlcIwh\GWjdHigZY5lKGOxbYDs[ZRmdHliYXLvcIl{cGWmIHPhd5Bie2ViMz:3JIFkfGm4aYT5JIlvKHKnc4DvcpNmKHSxIFHCWE0zPjNxMlTHM41mfG[xcn3pckBkd22kaX7heIlwdg>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ7MUG0OUc,OjN4OUGxOFU9N2F-
MCF-7  MkC2RZBweHSxc3nzJGF{e2G7 NYj4dHp1OjEQvF5CpC=> NY\i[4JjPzKq MojpbY5pcWKrdIOg[ZF2d2xvIHHu[EA1NU:KVD3t[YRq[XSnZDDhdI9xfG:|aYO= MkLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{W2OFMoRjJ|Nke1OlQ{RC:jPh?=
hCMEC/D3  NWL5XoJWSXCxcITvd4l{KEG|c3H5 NV7sSJBkOjYQvF2= NVnLfVJIPzKq NWX1[mZCemWmdXPld{BNfHiDIHnu[JVk\WRiYYDvdJRwe2m| M2jEXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nk[1NVk5Lz5{M{[2OVE6QDxxYU6=
Jurkat NFzPVYJCeG:ydH;zbZMhSXO|YYm= Mn;4NVIvPS13MN88US=> MmDuNYg> MYjkc5NmKGSncHXu[IVvfGy7IIP1dJBz\XO|ZYOgV3BJNWmwZIXj[YQhWGG{LUSgZ4xm[X[jZ3WsJHBCWlBiY3zlZZZi\2VuIFTORUBnemGpbXXueIF1cW:wLDDhcoQhdG:|czDv[kB3cWGkaXzpeJk> NXvQcGtXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFI6PzZpPkKzOFQzQTd4PD;hQi=>
CNE-1 MYDBdI9xfG:|aYOgRZN{[Xl? MXeyNO69VcLi NGDoOIozPGh? NEj4UI9qdmirYnn0d{BTSURyMEGtbY5lfWOnZDDj[YxtKGSnYYTo M3;B[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEK2PFUxLz5{M{SyOlg2ODxxYU6=
HONE-1 M1j6XGFxd3C2b4Ppd{BCe3OjeR?= MVGyNO69VcLi NVnRdW1LOjSq MkH6bY5pcWKrdIOgVmFFODBzLXnu[JVk\WRiY3XscEBl\WG2aB?= M1;IVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEK2PFUxLz5{M{SyOlg2ODxxYU6=
astrocytes cell M3TKemFxd3C2b4Ppd{BCe3OjeR?= MWe0NO69VQ>? MX62bC=> MX7y[YR2[2W|IHXhdox6KGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIP0ZZVzd3Oyb4LpcoU> MlzIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MUG3O|goRjJ|NEGxO|c5RC:jPh?=
U-937 NVuxTndTSXCxcITvd4l{KEG|c3H5 NUTvWoJXOTEQvF2= MV6zNI1qdg>? MkK0dJJmfmWwdIOgWG5HNWmwZIXj[YQhdmWlcn;weI9{cXN? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzMEe0PEc,OjN2MUC3OFg9N2F-
MDA-MB-231 MUjBdI9xfG:|aYOgRZN{[Xl? NYXxbVBsOTByzszN MoPONYg> NFq5[4VqdmirYnn0d{B{\W6|aYTpfoF1cW:wIITvJHRTSUmOIIXwc44hVVSGSDDkc5dvNXKnZ4XsZZRqd25? NFfHPI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwPFQzQSd-MkO0NFg1Ojl:L3G+
HeLa  NUHHOlhvSXCxcITvd4l{KEG|c3H5 MVmxNFDPxE1? NXf5UHM5Omh? MVjicI9kc3NiSmLTMVE2KEmwZIXj[ZMhSXCxcITveIlkKEOnbHygSIVifGh? M3LZeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{S0NFQ2Lz5{M{O0OFA1PTxxYU6=
Ec109 NUDVcoxVSXCxcITvd4l{KEG|c3H5 Ml21NVDPxE1? M1\Je|Zp NH:4NVVjdG:la4OgZZBweHSxdHnjJINwdWKrbnH0bY9vKG:oIGTheE1UdWGlTkegZY5lKHKjZHnheIlwdg>? NXm2NmV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzN|g2PjhpPkKzN|M5PTZ6PD;hQi=>
H460  Mo\CRZBweHSxc3nzJGF{e2G7 NUPuNGxNOTEQvF2= MUC2bC=> M3nNd4Jtd2OtczDhdI9xfG:2aXOgZ49u[mmwYYTpc44hd2ZiVHH0MXNu[WOQNzDhcoQhemGmaXH0bY9v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN|OEW2PEc,OjN|M{i1Olg9N2F-
HeLa  MVfBdI9xfG:|aYOgRZN{[Xl? MWO1NO69VQ>? MV2xMlVp Mk[2ZYJzd2ejdHXzxsBEcGyjbYnkbYEucW6mdXPl[EBieG:ydH;zbZM> NIrFcJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OwN|gxPCd-MkOzNFM5ODR:L3G+
SK-HEP1 NYrx[4JXSXCxcITvd4l{KEG|c3H5 NUfw[o5wOTByzszN MlvVNYg> MkmxbY5pcWKrdIOgR5JVOSCjY4TpeoF1\WRiY3HzdIF{\S1|LDCtO{whNThuID25MEBidmRicH;sfUhCTFBvcnnic5NmMSCyb3z5cYVz[XOn MkjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MEK2OVAoRjJ|M{CyOlUxRC:jPh?=
QGY7701 M1XuXGFxd3C2b4Ppd{BCe3OjeR?= MV2yOe69VQ>? M1jBNFEvPWh? MWLpcohq[mm2czDhZ4N2dXWuYYTpc44hd2Zic4XiMWcyKHCqYYPlJIlv\HWlZXSgZpkhTE:[IDugdZVmemOndHnu NYPNZXdSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOFAxPjFpPkKzNlQxODZzPD;hQi=>
HepG2 M3rEcGFxd3C2b4Ppd{BCe3OjeR?= MkO2NlDPxE1? NF\nb5M{OG2rbh?= NXzx[W04cW6qaXLpeJMhfGinIHXubIFv[2WmIHPlcIwh\GWjdHigZpkh[2:vYnnu[YQhfHKnYYTt[Y51KG:oIHHwbYdmdmmwIHHu[EBVWkGLTB?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{NEKzPUc,OjN{MkSyN|k9N2F-
U87  MnzERZBweHSxc3nzJGF{e2G7 NYiwXFdXOjYQvF2= MojNNog> M{HaZoRm[3KnYYPld{Bqe2:uaYH1bZJqfGmpZX7pckApUVOOKT3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYToMEBjfXRibn;0JI5m[3KxdHnjJINmdGxiZHXheIg> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{OU[yOkc,OjN{Mkm2NlY9N2F-
HSC-2 NVTnTXJNSXCxcITvd4l{KEG|c3H5 MlfWNlUwPTEQvF2= MWqxbC=> M{jxNIlvcGmkaYTzJHNPNTN6LXnu[JVk\WRiY4n0c5RwgGmlaYT5 MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF3NUK0PEc,OjNzNUWyOFg9N2F-
HSC-4 NXu3Z2xqSXCxcITvd4l{KEG|c3H5 MnjVNlUwPTEQvF2= NV\FN5RlOWh? M{HjcYlvcGmkaYTzJHNPNTN6LXnu[JVk\WRiY4n0c5RwgGmlaYT5 MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF3NUK0PEc,OjNzNUWyOFg9N2F-
CL-1 NFnP[GZCeG:ydH;zbZMhSXO|YYm= MYCyNO69VQ>? MVmxbC=> NIjhUmtjdG:la4OgU3A1PDliaX7keYNm\CClZXzsJIRm[XSq MknGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzM{G3PFIoRjJ|MUOxO|gzRC:jPh?=
MEL NI\KZ2dCeG:ydH;zbZMhSXO|YYm= M2jjSlIx|ryP MWWyOIg> MlnsSG1UVw>? MVLpcZBicXKnczDQNng4NWmwZIXj[YQhVUWOIHPlcIwh[XCxcITvd4l{ MkLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUS4PFcoRjJ|MEG0PFg4RC:jPh?=
Bel-7402  MXPDZZNx[XOnIFHjeIl3[XSrb36gRZN{[Xl? MXG1NO69VQ>? M2TGTVJp MoLUbY5pcWKrdIOgVGNGNWmwZIXj[YQh[W6xaXvpdy=> M3HESFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEC4O|QzLz5{M{CwPFc1OjxxYU6=
Eca-109 MXvBdI9xfG:|aYOgRZN{[Xl? NIfKNI4zPc7:TR?= NI\ad2Y{OG2rbh?= Mn;hbY5pcWKrdIOgRmouSjFzLXnu[JVk\WRiY3XscEBl\WG2aB?= NX\x[WVIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|Y6PjdpPkKzNFc3QTZ5PD;hQi=>
MEL NWThemJRSXCxcITvd4l{KEG|c3H5 M4rpWVIx|ryP M4PoVlFp NEDuVGVFVVOR NGPDelNqdXCjaYLld{BROlh5LXnu[JVk\WRiTVXMJINmdGxiYYDvdJRwe2m| NVfLXY46RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVQ5QDdpPkKzNFE1QDh5PD;hQi=>
Bel-7402  M2X4XWFxd3C2b4Ppd{BCe3OjeR?= MVO1NO69VQ>? MV6ybC=> M3zJdolvcGmkaYTzJHBETS2rbnT1Z4VlKGGwb3nrbZM> Mn7RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEi3OFIoRjJ|MEC4O|QzRC:jPh?=
L929 M37M[GZ2dmO2aX;uJGF{e2G7 MVSyMlXPxE1? M4DNfVFp MmLhbY5kemWjc3XzJHJKWDFiZYjwdoV{e2mxbjDhcoQh\XijY3XyZoF1\WRiVF7G{tEucW6mdXPl[EBucXSxY3jvcoRzcWGuIHT5d4Z2dmO2aX;uJIFv\CCUT2OgdJJw\HWldHnvci=> MoqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEC1NVgoRjJ|MECwOVE5RC:jPh?=
RCC  M1WyTmNmdGxiVnnhZoltcXS7IFHzd4F6 MWmxNFDPxE1? NGH4RmkzPGh? MkPrdoVkd3[ncnXzJJRp\SC4aXHibYxqfHlib3[gZ4VtdHNiZYjwc5Nm\CC2bzCxOYQuWEeMMh?= MkfqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OUG0PVQoRjJ{OUmxOFk1RC:jPh?=
NB2a/d1 NWXoZXVtSXCxcITvd4l{KEG|c3H5 M2TmdFExOM7:TR?= M1;VN|czcA>? MYXheJRmdnWjdHXzJJN1[XW{b4Pwc5JqdmVvaX7keYNm\CClYYPwZZNmKGGldHn2bZR6NCCSQWLQMEBidmRidHH1JINt\WG4YXfl Mn\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OEi1OFEoRjJ{OUi4OVQyRC:jPh?=
T cell M1LVN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHsPZQzPS1zMEFOwG0> MU[yOIg> NYTsSoo5\G:|ZT3k[ZBmdmSnboTsfUBqdmirYnn0[YQhXCClZXzsJJBzd2yrZnXyZZRqd25ibXXkbYF1\WRidHjyc5VocCC2aHWgZ48ue3SrbYXsZZRqd25id3n0bEBidnSrLVPEN{BidmRiYX70bU1ETDJ6 Mnj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OEK1N|goRjJ{OUiyOVM5RC:jPh?=
K562 M334TmFxd3C2b4Ppd{BCe3OjeR?= NIfj[IMyODEQvF2= M3H4TlFp NVmxOW9n[myxY3vzJGFjdm:kYY\pd4N2dcLiRj3pcoR2[2WmIHHwc5B1d3Orcx?= MnjXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{KzO|IoRjJ{OUeyN|czRC:jPh?=
Jurkat  M3\EdGFxd3C2b4Ppd{BCe3OjeR?= Mn\yOFDPxE1? M4fGSFFp NU\z[Zg6[WKxbHnzbIV{KE[jc1ytbY5lfWOnZDDjZZNx[XOnIHHjeIl3[XSrb36gZY5lKGOnbHyg[IVifGh? NXf5eYxZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5OFI4OzhpPkKyPVQzPzN6PD;hQi=>
BGC-823 M{XaWWFxd3C2b4Ppd{BCe3OjeR?= NFf2OpoyODEQvF2= M3;SRlFp M2SyfZBienSrYXzsfUBz\XOldXXzJINmdGy|IHHnZYlve3RiZHHtZYdmKG:oIHThbYR7\Wmw NFT1U4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlU1PSd-MkK5NlY2PDV:L3G+
Hep3B M1vLXmFxd3C2b4Ppd{BCe3OjeR?= MljROVDPxE1? NITQUlAycA>? NUX1WFV[[myxY3vzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEiHR1Pz NXrifndLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlMyPTRpPkKyPVI{OTV2PD;hQi=>
LLC-PK1 MknoRZBweHSxc3nzJGF{e2G7 NUTPSlR6OjEQvF2= NWnHTIZlOWh? M{XvOJBz\X[nboTzJINqe3CuYYTpck1qdmS3Y3XkJIRm\3KjZHH0bY9vKG:oIFH0[|UtKGKnY3zpck0yNCCjbnSgRZRoOTJicILveIVqdnN? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh7NkCzO{c,OjJ6OU[wN|c9N2F-
A549 M4LjVmFxd3C2b4Ppd{BCe3OjeR?= NXzFZZg4PTEQvF2= M1TYeFFp NFv0RVZjdG:la4OgeIhmKEKDST3pcoR2[2WmIHHwc5B1d3Orc9Mg NVmzVVlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4PFczOTVpPkKyPFg4OjF3PD;hQi=>
SGC-7901  MmfURZBweHSxc3nzJGF{e2G7 NXuwTnJmOTEQvF2= M2XvS|I1cA>? M{XEcIlvcGmkaYTzJO6zNM7{LXTpcYV1cHmuYXPyfYx{cGmtb37pck1qdmS3Y3XkJIFxd3C2b4Ppd:Kh M1PYSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OES4OVk4Lz5{Mki0PFU6PzxxYU6=
DM6 M1\aSGFxd3C2b4Ppd{BCe3OjeR?= M3HqUFExOM7:TR?= Ml3uO|Jp MkjxZoxw[2u|IHLveIghTTKILUGgZY5lKEV{RoTyMY1m\GmjdHXkJIN6fG:2b4jpZ4l1gQ>? NFvSNmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOVMzQCd-MkK4NlU{Ojh:L3G+
MCF-7, MDA-MB-468, Caco-2 MlKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnkNIE2OM7:TR?= NYW3bodCPDiq NFL0bVBqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBqdmS3Y3XkJIJ6KHOjcH;ubY4> NHHrT5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiwNFk3QCd-MkK4NFA6Pjh:L3G+
A2750  MojORZBweHSxc3nzJGF{e2G7 MXWyNO69VQ>? NVvMXlJZOmh? MWnEUXNQ NFXmTW1jdG:la4OgZ4F{eGG|ZTDjcIVifmGpZTDkeZJqdmdiaHXs[Y5idGmwIITy[YF1dWWwdDDhcoQhemWmdXPld{BifXSxcHjh[4lkKGOnbHyg[IVifGh? NFrxXXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke4OFM3Oyd-MkK3PFQ{PjN:L3G+
U87  NWPBblRiSXCxcITvd4l{KEG|c3H5 M{fNZlIx|ryP NWjPbIxFOjSq M{XSbJJm\HWlZYOgeIhmKGGyb4D0c5NqeyC{YYTlJIlv\HWlZXSgZpkhWEyDQh?= NVm3dIZyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3O|g4QDBpPkKyO|c5PzhyPD;hQi=>
HT1080 NGTQT4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\POGE2OM7:TR?= M{\EU|dl NVv0fYVQcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[2G3c3XkJIJ6KGOxbXLpcoVlKHS{ZXH0cYVvfCCxZjDER2Eh[W6mIF;NVC=> MlvwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEC5PFQoRjJ{N{SwPVg1RC:jPh?=
A549  Mni5RZBweHSxc3nzJGF{e2G7 NEO0O|E2OM7:TR?= NF3QbWszcA>? MnvwdIFzfGmjbHz5JIRm[3KnYYPld{B{d2SrdX2gd4Vt\W6rdHWtbY5lfWOnZDDhdI9xfG:|aYO= NGHNXGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyNVgxPCd-MkK3NlE5ODR:L3G+
Primary OPC NHTXSJFCeG:ydH;zbZMhSXO|YYm= M1r6[FHPxE1? M3vte|ZpNzJ2aB?= NFPmSlFz\WS3Y3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGy|IHnuJIViemy7LTDhcoQhdGG2ZT3hdI9xfG:|aYOvcoVkem:|aYO= NHrzdpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkewO|M5PSd-MkK3NFc{QDV:L3G+
PMNs MYfBdI9xfG:|aYOgRZN{[Xl? MYe0NO69VQ>? M3;FNVZp NGX4W|RFVVOR M1G2epJmfmW{c3XkJJRp\SCjbX;1cpQhd2ZiY3zlZZZm\CClYYPwZZNmNTNidH:gcoVieiC4ZXjpZ4xmKGyndnXsdy=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ7MkW3O{c,OjJ4OUK1O|c9N2F-
A549  NYfONWN5SXCxcITvd4l{KEG|c3H5 Mor4OVDPxE1? M4nqXFFp MnL0dJJmfmWwdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhSmGxaIXvd4ll\SCL NWSyPWVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2PFc3OzVpPkKyOlg4PjN3PD;hQi=>
AGS MXrBdI9xfG:|aYOgRZN{[Xl? M4HHXlIx|ryP MWixNog> M2Hke4lvcGmkaYTzJJRp\SCjY4TpeoF1cW:wIH;mJJBzdy2lYYPwZZNmNTNiaX6gdoV{eG:wc3WgeI8hfGinIFX0U2FkKG[{YXP0bY9v Moi3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4OEezPVgoRjJ{Nki3N|k5RC:jPh?=
shC9  NEPBWXdCeG:ydH;zbZMhSXO|YYm= M2DjV|Ex|ryP M4DMXlE3cA>? NIDpXIVjdHWwdIOgV2hJKGW6cILld5Nqd25iaX6gd4hEQSClZXzsd{BmgHCxc3XkJJRwKGWrdHjldkBRSSCxcjDMVGM> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2MUC5OEc,OjJ4NEGwPVQ9N2F-
primary MEFs NELZboRHfW6ldHnvckBCe3OjeR?= NEDHe3gyOmh? NGiyOYPDqGmwY4LlZZNmeyCndH;wc5Nq\GVvaX7keYNm\CCvaYTvZ4hwdmS{aXHsJI1mdWK{YX7lJIRmeG:uYYLpfoF1cW:w Mk\JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUO3OlcoRjJ{NkGzO|Y4RC:jPh?=
3T9 MEFs MWPGeY5kfGmxbjDBd5NigQ>? NX3ueG9EOT[q NYG2dJczcW6lcnXhd4V{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHHmeIVzKGW2b4Dvd4ll\S22cnXheI1mdnR? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzM{e2O{c,OjJ4MUO3Olc9N2F-
3T9 MEFs MnfKSpVv[3Srb36gRZN{[Xl? NYjxRWlvOTiq MkDWeZBz\We3bHH0[ZMhcW6rdHnheI9zKGOjc4Dhd4UuQSCkdYSg[I94dnKnZ4XsZZRmeyCnZn\lZ5RweiClYYPwZZNmeyCjZoTldkBmfG:yb4Pp[IUhfHKnYYTt[Y51 Mon3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUO3OlcoRjJ{NkGzO|Y4RC:jPh?=
MDA-MB-231  MX;BdI9xfG:|aYOgRZN{[Xl? M3rHWlIx|ryP MXu0[C=> NUSzVo9OemWmdXPld{B1cGViY3XscEBieG:ydH;zbZMhcW6mdXPl[EBjgSCvZYTmc5JucW5idILlZZRu\W62IIfhd{B{cWewaX\pZ4FvfGy7 M4S2[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUmzOFQyLz5{MkW5N|Q1OTxxYU6=
C6  MXXBdI9xfG:|aYOgRZN{[Xl? Ml;2NVDPxE1? MmrxNlRp NFPafJljdG:la4OgeIhmKHO3cIDy[ZN{cX[nIHXm[oVkfCCxZjD0bIUheGWydHnk[UBwdiC4aXHibYxqfHl? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6OEm4NEc,OjJ3OEi5PFA9N2F-
HL-60  Mlr1RZBweHSxc3nzJGF{e2G7 M3PLXlExOM7:TR?= NYD5OlFtOjSq MXvpcohq[mm2czD0bIUh[2WubDDhdI9xfG:|aYOgd4Vt\WO2ZXSgLEsqNW2nboTofYwh|rJvKEJiirI3MS2maXfseYNweHm{YX7vd4ll\cLiNR?= M4DwfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUS2OlY6Lz5{MkW0OlY3QTxxYU6=
HL-60 NXu2eXVQSXCxcITvd4l{KEG|c3H5 NGDLfmkyOC16MN88US=> NYHLb2ZtPGh? NGTtcm5qdmirYnn0d{BVT0iTLXnu[JVk\WRiY3XscEBieG:ydH;zbZPDqA>? M4ftTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUKzNlI6Lz5{MkWyN|IzQTxxYU6=
BCC NGfLUoJCeG:ydH;zbZMhSXO|YYm= NYmyRWFWPTEQvF2= MkHxNYg> Mon4bY5pcWKrdIOgSGFVWy2vZXTpZZRm\CCpcn;3eIghcW6qaXLpeIlwdg>? NH\pdo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxPVQ{Pid-MkK1NVk1OzZ:L3G+
RAW 264.7 MYPBdI9xfG:|aYOgRZN{[Xl? M4m0XVUxNzFyMN88US=> MVSxbC=> MXTjc45k\W62cnH0bY9vNWSncHXu[IVvfCCrbnjpZol1eyCGT16tbY5lfWOnZDDyVm5CKGOuZXH2ZYdm MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR7MUSyOkc,OjJ2OUG0NlY9N2F-
K562 MY\BdI9xfG:|aYOgRZN{[Xl? NH35SI0zPc7:TR?= M{nPcFJp MWTwdoV3\W62czDhdI9xfG:|aYOgbY5lfWOnZDDifUBkdy22cnXheI1mdnRid3n0bEBidXW{ZX7zbY4hTyCjbnSgWHJCUUx? NHztUm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4N|c4Pyd-MkK0PFM4Pzd:L3G+
SGC-7901 MmnKRZBweHSxc3nzJGF{e2G7 MWeyNEDPxE4EoB?= M{fsT|Jp MU\heJRmdnWjdHXzJGgzVzMEoH;yJHRPTiEQsT3pcoR2[2WmIHPlcIwh[XCxcITvd4l{KGG|IIflcIwh[XNiY3HzdIF{\S1|IHHjeIl3cXS7wrC= M2PDTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEexOVg6Lz5{MkS3NVU5QTxxYU6=
PC3 M4XQOGFxd3C2b4Ppd{BCe3OjeR?= M2T4TlEx|ryP NWS1dnF6PGh? NVfySJVE[2:3boTldpMh\myjdn;jc5hq\C2rbnT1Z4VlKGOjc4Dhd4UuemWuYYTl[EBieG:ydH;zbZM> NELBZWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS3NVk4PCd-MkK0O|E6PzR:L3G+
SMMC-7721 MX;BdI9xfG:|aYOgRZN{[Xl? M4PlUFUx|ryP MX60PIg> NEDzPWVifHSnboXheIV{KG:WUj3pcoR2[2WmIHHwc5B1d3Orcx?= NUHzU2VvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlU5OzNpPkKyOFY2QDN|PD;hQi=>
HeLa  MYTBdI9xfG:|aYOgRZN{[Xl? NGrMfJQ2OMLizszN MYi0M|hp NWXOWoxtcW6qaXLpeJMhW1SVLXnu[JVk\WRibHH0[U1xcGG|ZTDhdI9xfG:2aXOg[ZZmdnS| NI\oWYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS2NFUxPCd-MkK0OlA2ODR:L3G+
HeLa  Mn23RZBweHSxc3nzJGF{e2G7 MlXCOVDDqM7:TR?= M{exOFFp NV71bZRZe3WycILld5NmeyC2aHWgSnJCWC2rbnT1Z4VlKGGlY4XteYxifGmxbjDv[kBidm6neHnuJHYheG:|aYTpeoUh[2WubIO= M3LHe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES5OFQxLz5{MkS0PVQ1ODxxYU6=
T47D  MmT4RZBweHSxc3nzJGF{e2G7 NEDNUHEyODEQvF2= MWixbC=> M162ToJtd2OtczD0bIUh\2WwZYLheIlwdiCxZjDFMYNi\C:FVF[yJIJ6KFOWUx?= M{XDN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NECxNVY5Lz5{MkSwNVE3QDxxYU6=
HeLa  MnrNRZBweHSxc3nzJGF{e2G7 MVqzNO69VQ>? NGThRoI1cA>? NFSxbG/DqGmwY4LlZZNmeyC2aHWg[4Vv\XKjbDDj[YxtKH[rYXLpcIl1gSB2ODDoJIFnfGW{IIDoc5Rw\HmwYX3pZ{B1emWjdH3lcpTDqA>? MkflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|OUSyOFgoRjJ{M{m0NlQ5RC:jPh?=
HCC Mn;pRZBweHSxc3nzJGF{e2G7 NUP6clQzOjBizszNxsA> NV3iZoZDOmh? NYD4Z5dV[XS2ZX71ZZRmeyC2aHWgSGhCKGmwZIXj[YQh[WO2aY\heIlwdiCxZjDQRXJR MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2MkezNkc,OjJ|NEK3N|I9N2F-
mESCs MWLBdI9xfG:|aYOgRZN{[Xl? NIfrZlYzNjYQvF2= NYTLZ|BxOmh? M3HTU4lvcGmkaYTzJJRp\SCQYV[tcYVlcWG2ZXSgZ4F{eGG|ZTDhZ5RqfmG2aX;u NFTRS5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK4OVI4PCd-MkKyPFUzPzR:L3G+
EMT-6  M4HsUmN6fG:2b4jpZ4l1gSCDc4PhfS=> MmrLNVAx|ryP MnP2NYg> MXjwZZJ1cWGubImgZoxw[2unZDDj[YxtKGSnYYToJIlv\HWlZXSgZpkhe2m{YX3ld4lv\Q>? MlvsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NUG5NlEoRjJ{MkWxPVIyRC:jPh?=
MCF7 MkO1RZBweHSxc3nzJGF{e2G7 M13KSFUxyqEQvF2= NH;sXIcycA>? M4DS[4lvcGmkaYTzJHBCKGGldHn2ZZRm\CClYYPwZZNmNTNuID25MEBidmRicH;sfUhCTFBvcnnic5NmMSCyb3z5cYVz[XOn MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ{M{O0OUc,OjJ{MkOzOFU9N2F-
K562 M1rMUmFxd3C2b4Ppd{BCe3OjeR?= MXOyNEDPxE4EoB?= M1njU|Q5cA>? NUCxN|hsTE2VTx?= NEXQdnBjdG:la4OgcIFx[XSrbnniMYlv\HWlZXSgbY5pcWKrdHnvckBw\iC4aXHibYxqfHliYX7kJIFxd3C2b4Ppd{BqdmS3Y4Tpc44> M3:yVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkG2NVU5Lz5{MkKxOlE2QDxxYU6=
Molt4-hyg NGXWXFVCeG:ydH;zbZMhSXO|YYm= MmC2NVDPxE1? MXWwMlVp MWficI9kc3NiZnHycoV{d2xvaX7keYNm\CClYYPwZZNmNTNvbHnr[UBi[3Srdnn0fS=> NVnYVXVZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yO|U5OTFpPkK2Nlc2QDFzPD;hQi=>
HeLa MWrBdI9xfG:|aYOgRZN{[Xl? NX;Zbpl2OTEQvF2= M3fpeVAvPWh? M1i0W4lvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KHSqZTDjc41jcW6nZDD0doVifG2nboSge4l1cCCpb33pd4lvKE5iYX7kJHRTSUmO M3i3dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUe5OlYyLz5{MkG3PVY3OTxxYU6=
Jurkat T NGPkNnZCeG:ydH;zbZMhSXO|YYm= NXf2V2lvOzEQvF2= NHrZSYExNjWq NE\YVZdFVVOR NGDEOWZjdG:la4OgeIhmKHqrcnHtMYlv\HWlZXSgZZBweHSxc3nz NWXkS3NbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxOVk5QThpPkKyNVU6QDl6PD;hQi=>
Neutrophil MX3BdI9xfG:|aYOgRZN{[Xl? NF\rOFYzOM7:TR?= NHq2e5IxNjWq NE\ZNZdifHSnboXheIV{KHSqZTDwdo8u[XCxcITveIlkKGWoZnXjeEBw\iCPYWKx NHrMPZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG5Olg1PCd-Mk[xPVY5PDR:L3G+
HCT116 NHzmUVhCeG:ydH;zbZMhSXO|YYm= MojWOVDDqM7:TR?= MWiybC=> NEDTPVNz\X[ncoPld{B{gW6ncnfpd5Rq[yCjcH;weI9{cXNiZX\m[YN1eyCxZjDj[Yxm[2:6aXKgZY5lKE6SQz2xOuKh NH7TXZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkG1PVc2Oid-MkKxOVk4PTJ:L3G+
MDA-MB-231  MmLxRZBweHSxc3nzJGF{e2G7 MU[yMlUuPy53zszN M4fRSFJp M2HmWYlvcGmkaYTzJJRp\SClZXzsJIRm[XSqIH;mJG1FSS2PQj2yN|Eh[2WubIOgbY5lfWOnZDDifUBUTFRiaX6gZUBkd26lZX70doF1cW:wIHTldIVv\GWwdDDtZY5v\XJ? NGTy[oc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGxOVUzPid-MkKxNVU2OjZ:L3G+
LNCaP MoThRZBweHSxc3nzJGF{e2G7 NVTjNGRvPDEQvF2= MYGybC=> NVG3XW93cW6qaXLpeJMh[nW2ZXnuJIlv\HWlZXSgZ4VtdCCjcH;weI9{cXN? M1zKOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUG0O|Y1Lz5{MkGxOFc3PDxxYU6=
MB231 NHm1SldCeG:ydH;zbZMhSXO|YYm= NG\4XGcyODEQvF2= M{PtbFFp MYPEUXNQ MkTFZYJzd2ejdHXzJJRp\SCrbnT1Z5Rqd25ib3[gZ4VtdCCmZXH0bEBjgSCZRVWxJIlvcGmkaYTpc44tKFSUQVnMJJRz\WG2bXXueEwh[W6mIITo[UBkd22kaX7heIlwdg>? NV\tOIlTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNVI6PDBpPkKyNVEzQTRyPD;hQi=>
HCC38  MUjBdI9xfG:|aYOgRZN{[Xl? NVftWXI6OTByzszN NWTsd2EyOWh? M3LXb2ROW09? NFLQc3Ri[nKxZ3H0[ZMhfGinIHnu[JVkfGmxbjDv[kBk\WyuIHTlZZRpKGK7IGfFSVEhcW6qaXLpeIlwdixiVGLBTWwhfHKnYYTt[Y51NCCjbnSgeIhmKGOxbXLpcoF1cW:w MlfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMUK5OFAoRjJ{MUGyPVQxRC:jPh?=
MDA-MB231 NH[xZ5dCeG:ydH;zbZMhSXO|YYm= NHm3Uo42OMLizszN MnfHNlRp NXu4NmttTE2VTx?= MoTWZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NUTZV3lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNFE{OzVpPkKyNVAyOzN3PD;hQi=>
LNCaP M1fMcGFxd3C2b4Ppd{BCe3OjeR?= NE\SW|c2OMLizszN MlrXNlRp NYTiUnQxTE2VTx?= NGDTcohi[nKxZ3H0[ZMh[3W{Y4XtbY4ucW6mdXPl[EBk\WyuIHTlZZRp NVPkcJg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNFE{OzVpPkKyNVAyOzN3PD;hQi=>
HCT116 M{jpSWFxd3C2b4Ppd{BCe3OjeR?= NFXSNo42OMLizszN MX[yOIg> NIjNU|RFVVOR M4m1coFjem:pYYTld{BkfXKldX3pck1qdmS3Y3XkJINmdGxiZHXheIg> NXzOUoxbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNFE{OzVpPkKyNVAyOzN3PD;hQi=>
Ishikawa  MnHQRZBweHSxc3nzJGF{e2G7 M1vZNlI2|ryP NYfqSZpLOjSq M3jseZJm\HWlZYOgZ4VtdCCmZXH0bEBidmRiYYDvdJRwe2m|IHnu[JVk\WRiYomgRoFnKEFz NYHIdYU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwPFg6OThpPkKyNFg5QTF6PD;hQi=>
 YD-8  M3j5Z2Fxd3C2b4Ppd{BCe3OjeR?= MVyxNFDPxE1? M1HYOlFp NYXqRnBTcW6qaXLpeJMhWEyHTz3pcoR2[2WmIHHwc5B1d3Orcx?= NEX2Vmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4OlE5Oyd-MkKwPFYyQDN:L3G+
eosinophil  NVe3XoFiSXCxcITvd4l{KEG|c3H5 NH21dFU6OM7:TR?= NF3pXm4zPGh? MYfpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBH[XNiYX70bYJw\HoEoB?= MlLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{mzN|QoRjJ{MEe5N|M1RC:jPh?=
L929 NF;obo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU[yMlXPxE1? MnLsNlRp MWXpcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjDwMZA{QCCjbnSgUmYu|rqEIITvJIF2\22nboSgWG5H|rFvaX7keYNm\CCwZXPyc5B1d3OrczDhcoQh[XW2b4DoZYd6 MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{N{C5O{c,OjJyMkewPVc9N2F-
YD-8  M2q4NGFxd3C2b4Ppd{BCe3OjeR?= NGjHZncyODEQvF2= MofUNYg> Ml:3Zoxw[2u|IITo[UBIWy2KQ3ytbY5lfWOnZDDhdI9xfG:|aYO= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{MEC3PEc,OjJyMkCwO|g9N2F-
HBx NGnVU3BCeG:ydH;zbZMhSXO|YYm= NUjWWVh3OjYQvF2= MnHsOFhp NVzLcmdETE2VTx?= NXXtZVdFemWmdXPl[EBk\WyuIHTlZZRpKGmwZIXj[YQh[nliMz3NRS=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{MEC3PEc,OjJyMkCwO|g9N2F-
U937  MUjBdI9xfG:|aYOgRZN{[Xl? M{DOWVUxyqEQvF2= NFzHXokycA>? MYPpcohq[mm2czDISk1qdmS3Y3XkJIFxd3C2b4Ppd:Kh M2T1U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUm4O|MyLz5{MUm5PFc{OTxxYU6=
HL60 MVLBdI9xfG:|aYOgRZN{[Xl? MkDyOFDPxE1? NXXhVGxYPDCvaX6= NXrPNJFkTE2VTx?= NFjxcnFjdG:la4OgRm5FSyClb33wc5Vv\HNiaX7keYNmKGW6cH;zeZJmKG:oIIDoc5NxcGG2aXT5cJNmemmwZTDhcoQhTE6DIH\yZYdu\W62YYTpc44> M3jxblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUizNlk3Lz5{MUm4N|I6PjxxYU6=
M-14 MWTBdI9xfG:|aYOgRZN{[Xl? MUeyOe69VQ>? NGnHUJIxNjWq NYP3PFY2yqCrbnjpZol1eyCkb4ToJJRp\SClcoXk[UBmgHS{YXP0MUBidmRiY3;tdI92dmRvaX7keYNm\CCjcH;weI9{cXN? MojGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NUS5OVkoRjJzOUW0PVU6RC:jPh?=
SK-BR-3 M4naTWFxd3C2b4Ppd{BCe3OjeR?= NH\rZYo2OMLizszN M1z1c|Jp NWTzb2x{[myxY3vzJIFxd3C2b4TpZ{BFVkFiZoLh[41mdnSjdHnvckBqdmS3Y3XkJIJ6KGOxbXLpcoF1cW:wIH;mJHNONTF4NDDhcoQhemGmaXH0bY9v NGHjTVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmwNVM5Pid-MkG5NFE{QDZ:L3G+
MDA-MB-468 MlzzRZBweHSxc3nzJGF{e2G7 MV21NOKh|ryP NXTEbpppOmh? NGjjO2xjdG:la4OgZZBweHSxdHnjJGRPSSCocnHncYVvfGG2aX;uJIlv\HWlZXSgZpkh[2:vYnnuZZRqd25ib3[gV20uOTZ2IHHu[EBz[WSrYYTpc44> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlyMUO4Okc,OjF7MEGzPFY9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3; 

PubMed: 27191889     


With the addition of Z-VAD-FMK, levels of cleaved Caspase-3 and PARP1 were decreased.

Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II; 

PubMed: 27191889     


Levels of Beclin1, Atg5, Atg7 and LC3-II were decreased after IFN-γ stimulation. However, when IFN-γ was combined with the caspase inhibitor, levels of Beclin1, Atg5, Atg7 and LC3-II were not decreased as determined by the Western Blot test. 

p23 / Hsp90 / hTRET / MMP2; 

PubMed: 19740745     


Expression of Hsp90 client proteins during cell death. HeLa cells were preincubated with z-VAD-fmk (100 μM) for 1 h and exposed to various concentrations of tunicamycin (Tu, in μM) for 24 h. Cell extracts were prepared and analyzed by Western blotting. β-Actin was used as the loading control.

cyto C; 

PubMed: 17301139     


z-VAD-fmk did not inhibit cytochrome c release in RRV-infected MA104 cells (RRV: rhesus rotavirus). Mock- and RRV-infected MA104 cells either were not pretreated or were pretreated with 100 μM z-VAD-fmk for 2 h before RRV infection, and treatment was continued during infection. Fourteen hours after RRV infection, the cytosolic and heavy-membrane fractions were assayed for cytochrome c by Western blotting. Mock-infected MA104 cells were used as negative controls. Cox IV and tubulin were used as protein loading controls for the heavy-membrane and cytosolic fractions, respectively. Positions of molecular weight markers are indicated on the left.

27191889 19740745 17301139
Growth inhibition assay
Cell viability; 

PubMed: 12522090     


Inhibition of magnolol-induced apoptotic cell death by z-VAD-fmk. CH27 cells were treated with cell permeable broad-spectrum caspase inhibitor (z-VAD-fmk) 2 h prior to 80 μm magnolol treatment. Cell viability was estimated. ***P<0.001, compared with the 80 μM magnolol-treated group. 

12522090
Immunofluorescence
cytochrome c ; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Smac/DIABLO; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Endo G; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of EndoG together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

AIF; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of AIF together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

11726499 12941691
ELISA
IL-1β; 

PubMed: 26254306     


Cell-free medium supernatants were harvested at 5 h. IL-1β levels were measured by ELISA. Z-VAD-FMK was used as a positive control. Bars represent means ± sem of pooled results from 3 experiments performed in triplicate at different times.

26254306
In vivo

In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo[7].

Protocol

Cell Research:

[6]

- Collapse
  • Cell lines: human granulosa cell lines (GC1a, HGL5, COV434)
  • Concentrations: 50 μM
  • Incubation Time: 48 h
  • Method:

    To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h with etoposide (50 μg/ml) and/or Z-VAD-FMK (50 μM) under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: CD1 mice
  • Dosages: 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (198.93 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+35 %PEG 300+2%Tween 80+ddH2O
For best results, use promptly after mixing.
6mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage powder
in solvent
Synonyms Z-VAD(OMe)-FMK
Smiles CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

In vivo Formulation Calculator (Clear solution)

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Can you suggest an in vivo formulation of S7023 for animal studies?

  • Answer:

    For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

  • Question 2:

    What is difference between S7023 & S8102?

  • Answer:

    Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

Caspase Signaling Pathway Map

Related Caspase Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID