Z-VAD-FMK

For research use only.

Catalog No.S7023 Synonyms: Z-VAD(OH)-FMK

649 publications

Z-VAD-FMK Chemical Structure

CAS No. 187389-52-2

Z-VAD-FMK (Z-VAD(OH)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

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10mM (1mL in DMSO) USD 849 In stock
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Selleck's Z-VAD-FMK has been cited by 649 publications

Purity & Quality Control

Choose Selective Caspase Inhibitors

Biological Activity

Description Z-VAD-FMK (Z-VAD(OH)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Features A key compound for apoptosis studies.
Targets
Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro

Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U937 MnLDRZBweHSxc3nzJGF{e2G7 NF\td4s2OM7:TR?= NVXWbnJrPDiq NWW0NG5XemWmdXPld{Bk\WyuIHTlZZRpKGOjdYPl[EBjgSCVS1mtTWkhfHKnYYTt[Y51yqB? NF7pTVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOFA1Oyd-MkW4NlQxPDN:L3G+
HL-60 MnvKRZBweHSxc3nzJGF{e2G7 MVq1NO69VQ>? MlXNOFhp MmK3doVlfWOnczDj[YxtKGSnYYToJINifXOnZDDifUBUU0lvSVmgeJJm[XSvZX70xsA> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NEC0N{c,OjV6MkSwOFM9N2F-
DTK-SME M1q2bWFxd3C2b4Ppd{BCe3OjeR?= MlnXOVDPxE1? NFjBZXAzcA>? MX;EUXNQ NX\2SZFoeGG{dHnhcIx6KGmwaHnibZR{KHSqZTDhdI9xfG:|aYOgbY5lfWOnZDDifUBjfXCrdnHjZYlv\Q>? NVHYOZg3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OFM5QTdpPkK1PFQ{QDl5PD;hQi=>
HCT116  M3fyV2Fxd3C2b4Ppd{BCe3OjeR?= M3\RRVUx|ryP M2nMO|Jp NWDz[Y56cW6qaXLpeJMhfGinIHPlcIwh\GWjdHigbY5lfWOnZDDifUBFTF[S M2XnVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OE[4PFE5Lz5{NUi2PFgyQDxxYU6=
SGN M4rrWmFxd3C2b4Ppd{BCe3OjeR?= NHnFeIQzOG2P MWW0PIg> NVzKRmdbcGG|IH7vJIlv\my3ZX7j[UBwdiCDSV[sJINidHCjaX6g[ZhxemW|c3nvckBweiClZXzsJIFxd3C2b4Ppdy=> Ml\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{S2N|MoRjJ3OEe0OlM{RC:jPh?=
A549/V16 M1WxT2Fxd3C2b4Ppd{BCe3OjeR?= NHjmSW82OM7:TR?= NHP0NHkycA>? MnH6doVxemW|c3XzJHRIN1SPLXnu[JVk\WRiYYDvdJRwe2m| NYTGeGk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OFYxOzNpPkK1PVQ3ODN|PD;hQi=>
U1 NEjkblBMcW6jc3WgRZN{[Xl? MVuxNFDPxE1? NX\r[5pLOmh? MYfpcohq[mm2czDjZZNx[XOnLUO= NUTsNFFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFA6PDJpPkK1PVgxQTR{PD;hQi=>
ACH-2 M1;4UGFxd3C2b4Ppd{BCe3OjeR?= M3zT[FAuOjByzszN MnLlNog> MXPy[YR2[2W|IHTyeYcucW6mdXPl[EBieG:ydH;zbZMh[W6mIIP1ZpNmeXWnboSgTGlXNTFicnXwcIlk[XSrb36gbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NEHkTlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4NFk1Oid-MkW5PFA6PDJ:L3G+
U1 NGHXdIdCeG:ydH;zbZMhSXO|YYm= M{TQT|AuOTByzszN MmDZNog> NWf2bW1temWmdXPld{BlenWpLXnu[JVk\WRiYYDvdJRwe2m|IHHu[EB{fWK|ZYH1[Y51KEiLVj2xJJJmeGyrY3H0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M4TiNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUiwPVQzLz5{NUm4NFk1OjxxYU6=
HUVEC-2c MXzBdI9xfG:|aYOgRZN{[Xl? NE\oNJI2OM7:TR?= NHzNSoY3cA>? M4i5Z4Rm[3KnYYPl[EB1cGVib4itUGRNNWmwZIXj[YQh[XW2b4DoZYd6 NXXmZ|RbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNlE4OjlpPkK2NFIyPzJ7PD;hQi=>
SP2/0 MVPBdI9xfG:|aYOgRZN{[Xl? NEnvZ3MyODEQvF2= NH3E[HcycA>? MkH1SG1UVw>? NYC0Spli[myxY3vzJJRp\SCjcH;weI9{cXNib3[gV3AzNzBiY3XscJM> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB5NEezNkc,OjZyN{S3N|I9N2F-
CEF M{\XRWtqdmG|ZTDBd5NigQ>? M2T5NVAtOzNuNkesNVAx|ryP M3O0b|E2dWmw MYLkc5dvNXKnZ4XsZZRmeyCSUjDlcpp6dWViYXP0bZZqfHlidH:gOFDwxIViYYSgNVAx|ryP MkC2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzMEKzN|koRjJ4MUCyN|M6RC:jPh?=
BEL-7402 NIrSZ21CeG:ydH;zbZMhSXO|YYm= NHn1WJIzOM7:TR?= NWjSS2VUOWh? NUD5WXJt[XS2ZX71ZZRm\CC2aHWgZZBweHSxdHnjJIlv\HWldHnvckBw\iCLSVmtNVDDqA>? M3i2d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MU[0O|k2Lz5{NkG2OFc6PTxxYU6=
HepG2 MkH2RZBweHSxc3nzJGF{e2G7 M3L6NVIx|ryP NHPBbo4ycA>? MYjheJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMg NUf4U2dZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xOlQ4QTVpPkK2NVY1Pzl3PD;hQi=>
HGL5 M4rXO2Fxd3C2b4Ppd{BCe3OjeR?= NXPMWo1bPTEQvF2= MlrhNlRp M33Mb4lv\GmlYYTld{BiKHC{b4TlZ5RqfmViZX\m[YN1KGGpYXnud5Qh\XSxcH;zbYRm Mlz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkmwO|UoRjJ4MU[5NFc2RC:jPh?=
GC1a MXvBdI9xfG:|aYOgRZN{[Xl? MWi1NO69VQ>? M4TxVlI1cA>? M1;KRolv\GmlYYTld{BiKHC{b4TlZ5RqfmViZX\m[YN1KGGpYXnud5Qh\XSxcH;zbYRm MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF4OUC3OUc,OjZzNkmwO|U9N2F-
A549 MmfvRZBweHSxc3nzJGF{e2G7 MmXaNlAh|ryP NXT3XplOOjSq MkXYdoVlfWOnczDj[YxtKGSnYYToJINifXOnZDDifUBJTkOSIITy[YF1dWWwdB?= MmfKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5OUSxOFkoRjJ3N{m0NVQ6RC:jPh?=
HeLa MVfBdI9xfG:|aYOgRZN{[Xl? MYexNO69VQ>? MkPUOFhp NXS3V204\GWlbHnu[ZMhfGinIILheIUhd2ZiYYDvdJRwe2m|IHTyZY1ifGmlYXzsfS=> M1XWcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{eyOVQ2Lz5{NUe3NlU1PTxxYU6=
SGC-7901 MoTuRZBweHSxc3nzJGF{e2G7 MXyyNOKh|ryP MmfpNYg> M3S0RYlvcGmkaYTzJJRp\SClZXzsJIRm[XSqIHnu[JVk\WRiYomgc5hidGmybHH0bY4> MkjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkewO|YoRjJ3N{[3NFc3RC:jPh?=
MCF-7 M3PwRmFxd3C2b4Ppd{BCe3OjeR?= MUixNO69VQ>? M1znc|Jp M3fifIlvcGmkaYTzJJRp\SClZXzsJIRm[XSqIHnu[JVk\WRiYomgN2I> M3PqOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{KyNVE1Lz5{NUeyNlEyPDxxYU6=
A549 MV\BdI9xfG:|aYOgRZN{[Xl? NIrDd4MyOCEQvF2= MX[yOIg> M3XCXGROW09? NF7KWFBxem:vb4Tld{BJSkNvdILlZZRm\CCDNUS5JINmdGxic4Xyeol3[WxiJjDheJRmdnWjdHXzJJRp\SClbHXheoVlKFCDUmCg[ZhxemW|c3nvci=> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6M{W2PEc,OjV4OEO1Olg9N2F-
U87 NIfwWZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnVXFI2OM7:TR?= NVfpWYFGOWh? MVPy[YNwfmW{czDj[YxtKGe{b4f0bEBnem:vIGTNXkB1emWjdH3lcpQ> M4PHNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkixOlY5Lz5{NU[4NVY3QDxxYU6=
Neocortical Neuron NXWxdohMVmW3cn;0c5hq[2m2eTDBd5NigQ>? MUixNFDPxE1? MWOxbC=> NYXTNnJ7[W62YXfvcol7\XNiSH;pZY1q\GViQT3JcoR2[2WmIF7leZJwfG:6aXPpeJk> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ5NUCwNUc,OjV4N{WwNFE9N2F-
Primary human placental cytotrophoblasts MXXBdI9xfG:|aYOgRZN{[Xl? MYmzNO69VQ>? NV;3W3QxOjSq NVniVXgxTE2VTx?= NUfPVFl6emW4ZYLz[ZMhfGinIHnubIljcXSrb36gc4YhOTIQsj3IV2QzKGK7IITybYNtd3OjbtMg MoPnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4NEK1PVIoRjJ3NkSyOVkzRC:jPh?=
MM NV;Y[oRvSXCxcITvd4l{KEG|c3H5 M1L3R|Ux|ryP M{X6flIxdWmw MmLXdIFzfGy7IHnubIljcXS|IGPIT{1qdmS3Y3XkJEBk\WyuIHTlZZRp M1nTeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUOwNFk5Lz5{NUWzNFA6QDxxYU6=
DLD1 M{THbmFxd3C2b4Ppd{BCe3OjeR?= M2LFe|IxyqEQvF2= MUixbC=> NIjWfm9x[XK2bImgdoV3\XK|ZYOgZ4VtdCCjcH;weI9{cXNiY3H1d4VlKGK7IGfTVFE> M3vpbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUK0NlQ3Lz5{NUWyOFI1PjxxYU6=
MDA-MB-231 NWfL[G1yS3m2b4TvfIlkcXS7IFHzd4F6 M1q5NlEx|ryP M2H3SVFp M2fHSoF2\22nboTzJINmdGxiZHXheIgh[W[2ZYKgR2hWSSC2cnXheI1mdnR? NG\MN5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWyNVUxOSd-MkW1NlE2ODF:L3G+
MKN28 MYnBdI9xfG:|aYOgRZN{[Xl? MV2xNO69VQ>? MXOzNI1qdg>? M4m1VmROW09? MWTpcohq[mm2czDUUmYu|rFicHz1d{BEUFhvaX7keYNm\CCjcH;weI9{cXN? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTVzM{m2NEc,OjV3MUO5OlA9N2F-
HL-60 M2LudmN6fG:2b4jpZ4l1gSCDc4PhfS=> NHTPR|kyODEQvF2= MYOxbC=> NETWcHBFVVOR MUVCpIlueHKxdnXzJJZq[WKrbHn0fUBw\iCWQ17Bd{11emWjdHXkJINmdGy| M3fSWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUCyPVMzLz5{NUWwNlk{OjxxYU6=
AGS MlroRZBweHSxc3nzJGF{e2G7 MXixNO69VQ>? M1zXV|Fp M1XlXZBz\X[nboTzJIN2emO3bXnuMYlv\HWlZXSgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[U0{NCBvODygZY5lKC17IIDyc5RmcW6| NIPwS|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5NlIyPCd-MkW0PVIzOTR:L3G+
A549  MmX5RZBweHSxc3nzJGF{e2G7 MVGxNO69VQ>? NH;WVGYzPGh? NIfDUnNjdG:la4OgSG1CWy2rbnT1Z4VlKGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzDhcoQhWEGUUDDhcoQh[XCxcITveIlkKGOnbHyg[IVifGh? NGXBOXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOFk5QSd-MkW0N|Q6QDl:L3G+
INS-1 M1r0Z2Fxd3C2b4Ppd{BCe3OjeR?= MYi1NO69VQ>? MUK2bC=> MYfEUXNQ MmrI[IVkemWjc3XzJJRp\SCycn;wc5J1cW:wIH;mJIViemy7IHHwc5B1d3SrYzDj[Yxte8Li MnjXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{C4PVcoRjJ3NEOwPFk4RC:jPh?=
Primary hepatocytes  NGrW[VBCeG:ydH;zbZMhSXO|YYm= M2jCPVUx|ryP MU[xPIg> MlPXbY5pcWKrdIOgZZBweHSxc3nzJI9nKGincHH0c4N6fGW|IHnu[JVk\WRiYomgRYN1KERiYX7kJHRPTi4QsR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRyN{WzPEc,OjV2MEe1N|g9N2F-
L929-A  MV3DfZRwfG:6aXPpeJkhSXO|YYm= MlexNlDDqM7:TR?= NHW0dm0zPGh? NYfKepNKcW6qaXLpeJMhXE6IzsGtbY5lfWOnZDDj[YxtKGSnYYTo M{LBVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{m4OVQxLz5{NUO5PFU1ODxxYU6=
L929-N MnzQR5l1d3SxeHnjbZR6KEG|c3H5 MkHGNlDDqM7:TR?= NXi5TGtxOjSq MVLlcohidmOnczDk[YF1cCC4aXGgZZV1d2O{aX7lJHRPTs7zIIDyc4R2[3Srb36= NI\3fYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO5PFU1OCd-MkWzPVg2PDB:L3G+
Cytotoxicity Assay NITVbGZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? Mle0NlDPxE1? NGrkN4s1QGh? MXPwdoV3\W62IF3IUWQucW6mdXPl[EBk\WyuIHTlZZRp NGLaWFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO5NlEyPid-MkWzPVIyOTZ:L3G+
COS7 Mn61T4lv[XOnIFHzd4F6 NF7uflQyOM7:TR?= MkLsNlRp NHLxOodqdmO{ZXHz[ZMh[2G|cHHz[UA{NzdiYXP0bZZqfGmncx?= M{fNe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{i0NFI2Lz5{NUO4OFAzPTxxYU6=
COS7 MmnTRZBweHSxc3nzJGF{e2G7 MUOxNO69VQ>? NXfOOmxMPDiq M{m0dpBienSrYXzsfUBxemW4ZX70[YQhTkNzMEGtbY5lfWOnZDDj[YxtKGSnYYTo NELZ[WM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO4OFAzPSd-MkWzPFQxOjV:L3G+
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A375 MUDBdI9xfG:|aYOgRZN{[Xl? M1TDWVIx|ryP NH7ie2kzPGh? NUHQTo51emW4ZYLz[ZMh\GmlaYTybY5wdmViQj3pcoR2[2WmIHHwc5B1d3Orcx?= M{\FN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nkm5NVEyLz5{NE[5PVEyOTxxYU6=
HDPC NFrnO25CeG:ydH;zbZMhSXO|YYm= NUXtTmNqPTEEtV2= MXeyOIg> M4DRN4lvcGmkaYTzJG5QNWmwZIXj[YQh[XCxcITvd4l{ NIjUOnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zOFU6Oyd-MkS2N|Q2QTN:L3G+
A549 Mnf3RZBweHSxc3nzJGF{e2G7 MYK1NOK2VQ>? MmLBNog> Mkn4SG1UVw>? M{jtdpBz\X[nboTzJJRp\SCqeYDv[IlxdG:rZDDEUmEh[2:wdHXueEBxcGG|ZTDpcoR2[2WmIHL5JINmeGijbH;jbJJwdWmw MmDtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OEixN|UoRjJ2NUi4NVM2RC:jPh?=
 BV-2 MV\BdI9xfG:|aYOgRZN{[Xl? NHO3e5czOM7:TR?= MlrrNog> NXTiSnBje3WycILld5NmeyCXVj3pcoR2[2WmIHPodo9u[XSrbjDlfJRzfXOrb36gZY5lKGSrbHH0bY9vKG:oIITo[UBvfWOuZXHyJIVvfmWub4Dl NYHrU4lCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1OVgyOThpPkK0OVU5OTF6PD;hQi=>
HT-29  NHvSS|JCeG:ydH;zbZMhSXO|YYm= M{XmUVUx|ryP MoPEOFhp MWDicI9kc3NidHjlJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGujZX3w[oVzd2x? MofZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NEmxO|UoRjJ2NUS5NVc2RC:jPh?=
A549 NF;LTWhCeG:ydH;zbZMhSXO|YYm= MXG1{txO M1zVOVI1cA>? M{HhOJN2eHC{ZYPz[ZMhPmYEoDjCT|ExODRyKT3pcoR2[2WmIHHwc5B1d3Orc9Mg M2\TWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUK5PFcyLz5{NEWyPVg4OTxxYU6=
MIA-PaCa-2 NHuycI9CeG:ydH;zbZMhSXO|YYm= MY[yOIg> M1LkdIJtd2OtczDjcIVifmGpZTDv[kBk[XOyYYPlMVMhcW6mdXPl[EBjgSCkb4ToJGEuPDR|NkW0JIFv\CCyYXPsbZRigGWu MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzMEm5Nkc,OjR3MUC5PVI9N2F-
Caki-1 MV7BdI9xfG:|aYOgRZN{[Xl? MV[yNO69VQ>? MmjPNYg> NWrqeIVlcW6qaXLpeJMhW0OSLXnu[JVk\WRiYYDvdJRwe2m| M4XnelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUC0OlgyLz5{NEWwOFY5OTxxYU6=
CLL NH3rN4JCeG:ydH;zbZMhSXO|YYm= MlSwNlXPxE1? MnjCNYg> NW\vUWZNeGG{dHnhcIx6KGKub3Prd{BOVE5{MkO4MYlv\HWlZXSgZ4VtdCCjcH;weI9{cXN? M3O1b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NE[3OlM1Lz5{NES2O|Y{PDxxYU6=
SGC-7901 NHLBeXhCeG:ydH;zbZMhSXO|YYm= MV[xNO69VQ>? NX\FNG5kOjSq MXzwdo9ud3SnczD0bIUhS0eLST3pcohq[mm2ZXSgZ4VtdCCpcn;3eIghcW5iU1fDMVc6ODFiY3XscJM> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3NES4PEc,OjR2NUS0PFg9N2F-
k1735 M{nubmFxd3C2b4Ppd{BCe3OjeR?= MV2yNQKBkc7:bR?= NVLmdFJWPGh? M{G1UIlvcGmkaYTzxsBU[Wyvb37lcIxiNWmwZIXj[YQh[XCxcITvd4l{ MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3MUGxOkc,OjR2NUGxNVY9N2F-
HaCaT  MUnBdI9xfG:|aYOgRZN{[Xl? NX\2bZRxOTByzszN NHPqRlQycA>? M1HPeYJtfW62czDVWmIucW6mdXPl[EBieG:ydH;zbZMhcW5iSHHDZXQh[2WubIO= M4T3fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W2PVk4Lz5{NEO1Olk6PzxxYU6=
podocytes NYrGO25ySXCxcITvd4l{KEG|c3H5 MlvTNlAx|ryP MWm2bC=> MX\pcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUA{NDRvRFfF NWHRUIp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzN|c4PzdpPkK0N|M4Pzd5PD;hQi=>
 KB M{jj[mFxd3C2b4Ppd{BCe3OjeR?= NXjWNGZoPTEQvF2= M33CR|I1cA>? M1nxdIlvcGmkaYTzJGxq[29vQT3pcoR2[2WmIHPhd5Bie2VvMzDhcoQhWEGUUDDhZ5RqfmG2aX;u MkDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|M{e0PVIoRjJ2M{O3OFkzRC:jPh?=
 HepG2  M3TTPGFxd3C2b4Ppd{BCe3OjeR?= M2jRPVEx|ryP M3m3flI1cA>? M{f2eJJm\HWlZYOgeolu\W62aX6gZ4xm[X[jZ3WgZ4F2e2WmIHL5JGxRWw>? NHvhdHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyOVgyPid-MkSzNlU5OTZ:L3G+
fetal rat lung fibroblasts  NIjYW|hMcW6jc3WgRZN{[Xl? NHnEe5o5OM7:TdMg M{nZVlFp NUTtOW5FcW6qaXLpeJMhS2G|cHHz[U0{KGGldHn2bZR6yqB? MnrqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUSxN|UoRjJ2M{G0NVM2RC:jPh?=
PDL fibroblasts NVH2UohoU2mwYYPlJGF{e2G7 NETyRm45OM7:TdMg NVrVU4xTOWh? MkH1bY5pcWKrdIOgR4F{eGG|ZT2zJIFkfGm4aYT5xsA> M3TOXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G0NVM2Lz5{NEOxOFE{PTxxYU6=
MB49 NGTKUZhCeG:ydH;zbZMhSXO|YYm= MmDSNlDPxE1? M{fQPFFp NV\zb41oemW4ZYLz[ZMhS01vaX7keYNm\CClZXzsJIRm[XSq MnLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OEK0N|MoRjJ2MkiyOFM{RC:jPh?=
5637 NEDwVWdCeG:ydH;zbZMhSXO|YYm= NWDneGszOjEQvF2= NEG2SGkycA>? NYHtTo9YemW4ZYLz[ZMhS01vaX7keYNm\CClZXzsJIRm[XSq NVGwXYc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFI1OzNpPkK0NlgzPDN|PD;hQi=>
A549  NYiyR4JwSXCxcITvd4l{KEG|c3H5 MVmxNFDPxE1? NIfMT481QGh? Mn34d5VxeHKnc4Pld{B1cGViYYDvdJRwe2m|IHPheZNm\CCkeTDwbZBmemmwZdMg M1\BWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkeyNlAyLz5{NEK3NlIxOTxxYU6=
MCC-2 NUD6eGtiSXCxcITvd4l{KEG|c3H5 NH\zPFUzOCEQvF2= MkDOOog> MlrNSG1UVw>? MYnicI9kc3NiaX7keYNm\CCkeTDzeIF2em:|cH;ybY5mKGGyb4D0c5NqeyClZXzsJIRm[XSq MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4Mk[1PEc,OjR{NkK2OVg9N2F-
ADF MnvhRZBweHSxc3nzJGF{e2G7 Mlj5OFDPxE1? MVy2bC=> MoDGbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkh[2inbX;0bIVz[XC7IIDseZMhYm6FbEK= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{OEKzNkc,OjR{MkiyN|I9N2F-
 U373  MlHwRZBweHSxc3nzJGF{e2G7 MYq0NO69VQ>? M3\Yd|Zp NFS3UIRqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClaHXtc5Rp\XKjcImgdIx2eyCcblPsNi=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{OEKzNkc,OjR{MkiyN|I9N2F-
RKO-HIPK2i Mnv6RZBweHSxc3nzJGF{e2G7 NUTtW|Y6PDEQvF2= Mm\TOog> NVTqcHRmcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliY3jlcY91cGW{YYD5JJBtfXNiWn7DcFI> M2jCSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkK4NlMzLz5{NEKyPFI{OjxxYU6=
HEC-1B Mk\iRZBweHSxc3nzJGF{e2G7 MU[yNO69VQ>? NX3oPG1IOWh? Mn\qdoVlfWOnczDUVE1qdmS3Y3XkJIFxd3C2b4Ppd:Kh[2G|cHHz[U0{KGGwZDDjZZNx[XOnLUm= NVTjb5NJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVM{PThpPkK0NlE{OzV6PD;hQi=>
4T1 NXHwWGNmS2WubDDWbYFjcWyrdImgRZN{[Xl? NF;vWmUzNjVvMUFOwG0> MXS0bC=> NYC5RpB1TE2VTx?= MUjy[ZNkfWW|IITo[UBkgXSxdH;4bYNqfHlib3[gOHQyKGOnbHzzJIlv\HWlZXSgZpkhW1CGVDDpckBiKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70JI1idm6nch?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJyNkG2NUc,OjR{ME[xOlE9N2F-
OS  M1[2V2Fxd3C2b4Ppd{BCe3OjeR?= M4\IV|Ix|ryP MWq0bC=> NHPLW|RqdmirYnn0d{B1cGViY3XscEBl\WG2aDDjZZV{\WRiYomgVGRV NHLCfG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwOFk{Pyd-MkSyNFQ6Ozd:L3G+
RPE MVjBdI9xfG:|aYOgRZN{[Xl? NFz3NJgyODEQvF2= MXq0PIg> M{DUe5BienSrYXzsfUBqdmirYnn0d{B1cGViY3HsdIFqdi1zIHHu[EAuOiCjY4TpeoF1cW:wIHHzJJdmdGxiYYOgeIhmKGOjc4Dhd4Uh[WO2aY\heIlwdg>? NEfMW5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwNlA2Oid-MkSyNFIxPTJ:L3G+
UD29a NWP2V5RFSXCxcITvd4l{KEG|c3H5 MkX1OVDPxE1? NVvmcnVIOjSq NHXTR2ZqdmirYnn0d{B1cGViY3XscEBl\WG2aDDjZZV{\WRiYomgUnVVOw>? NVTrdnE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxPVA2PzRpPkK0NVkxPTd2PD;hQi=>
SH-SY5Y  MXHBdI9xfG:|aYOgRZN{[Xl? NVjTU5F2OTEQvF2= M3H6UFI1cA>? MmTkZZR1\W63YYTld{Bk[XOyYYPlJIFkfGm4YYTpc44h[W6mIHPlcIwh\GWjdHigbY5lfWOnZDDifUBJVkVvMlTH NWfNclF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFU1PjNpPkK0NVQ2PDZ|PD;hQi=>
HeLa  MojIRZBweHSxc3nzJGF{e2G7 NInCTI0yOC9{MN88US=> MoLYNlRp NVHS[oZ5cW6qaXLpeJMhfGinIHHjeIl3cXS7IH;mJJRp\SCvYXrvdol1gSCxZjD0bIUhdWWvYnXyd{Bw\iC2aHWgZ4F{eGG|ZT3mZY1qdHl? M2roelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO3NlY3Lz5{NEGzO|I3PjxxYU6=
SCCVII  NEXGSlhCeG:ydH;zbZMhSXO|YYm= MVWyOe69VQ>? M{jIbVFp NVLzeZp3cW6qaXLpeJMhfGinIHPlcIwhc2mubHnu[{Bi\nSncjDkZZNifGmwaXK= NXzvW|d3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlY1PjRpPkK0NVI3PDZ2PD;hQi=>
RAW 264.7 NUjxU|BpSXCxcITvd4l{KEG|c3H5 NIr6NGEzOM7:TR?= MmHYNVhp MYLpcoNz\WG|ZYOgUGM{NUmLL98yMYFkfGmwIILheIlwKGOxbYDhdoVlKHSxIFXDWHYuVU:VIITy[YF1dWWwdDDvcox6 NV\uTWM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVY4ODdpPkK0NVE3PzB5PD;hQi=>
A549  Mk\5RZBweHSxc3nzJGF{e2G7 NEjoU5gzNjYQvF2= MYKyOIg> NFLkZ3Fl\WO{ZXHz[ZMhd3KrZH;ubY4ucW6mdXPl[EBifXSxcHjh[5kh[XNid3XscEBidmRiTH;zd{Bw\iEQlN-IcUBidHOxIH;jZ5VzemWmIHT1dolv\yCjdYTvdIhi\2mlIIDyc4Nme3N? NHW2enc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwNlUzOid-MkSxNFI2OjJ:L3G+
UM-SCC-10A  MYXBdI9xfG:|aYOgRZN{[Xl? MkfCOVDDvU1? NYLG[41iOmh? M4m2dZJm\HWlZYOgZZBweHSxc3nzJIlv\HWlZXSgZpkhcGmpaD3kc5NmKGm|b3HsZY51d2yjY4TvcoU> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEe1N{c,OjRyOUi3OVM9N2F-
BGC-823 M2q5b2Fxd3C2b4Ppd{BCe3OjeR?= M4mzVlExKM7:TR?= NXTUPY5qOjSq MYPy[YR2[2W|IITleJJidmS{aX7lMYlv\HWlZXSgZZBweHSxc3nzxsA> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7OEWxNUc,OjRyOUi1NVE9N2F-
Nalm-6  M{TEOmFxd3C2b4Ppd{BCe3OjeR?= NYPJVoVIOjEQvF2= M{juNFRp MXfpcohq[mm2czDjZZNx[XOnLUigZY5lKGOjc4Dhd4UuOyCjY4TpeoF1cW:wIHHu[EBRSVKSLUGgZ4xm[X[jZ3W= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB|OUm2O{c,OjRyM{m5Olc9N2F-
KHOS  MlvHRZBweHSxc3nzJGF{e2G7 NGK1[WY1OM7:TR?= NIjab2gzPGh? Mn6xZoxw[2u|IITo[UBqdmO{ZXHz[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgRmJOTDQEoB?= M3LDPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK1N|YyLz5{NECyOVM3OTxxYU6=
G292 MnPnRZBweHSxc3nzJGF{e2G7 Moi0OFDPxE1? NF7xd2YzPGh? MX7icI9kc3NidHjlJIlv[3KnYYPl[EBkdGWjdnHn[UBw\iClYYPwZZNmNTNiYX7kJHBCWlBiaX7keYNm\CCkeTDCRm1FO8Li NXrPOWxsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlU{PjFpPkK0NFI2OzZzPD;hQi=>
MG-63 Mnv6RZBweHSxc3nzJGF{e2G7 M3S1elQx|ryP M{L6OlI1cA>? NIi2WI5jdG:la4OgeIhmKGmwY4LlZZNm\CClbHXheoFo\SCxZjDjZZNx[XOnLUOgZY5lKFCDUmCgbY5lfWOnZDDifUBDSk2GM9Mg NEjuTIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyOVM3OSd-MkSwNlU{PjF:L3G+
EBV-transformed B cells NUjRT|BXSXCxcITvd4l{KEG|c3H5 MnLHNlDPxE1? NXmwTlg1Omh? Ml;uSG1UVw>? M3XYTIJtd2OtczDhcpRqNUOGOECgZY5lKGGwdHmtR2Q5PiCjboTpZo9lgS2rbnT1Z4VlKGGyb4D0c5Nqew>? NHHvWpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECwPFYzQCd-MkSwNFg3Ojh:L3G+
IM-9 B NULaUZZSSXCxcITvd4l{KEG|c3H5 MXGyNO69VQ>? NU\3[GlZOmh? M2f1TmROW09? MWnicI9kc3NiYX70bU1ETDhyIHHu[EBidnSrLVPEPFYh[W62aXLv[JkucW6mdXPl[EBieG:ydH;zbZM> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDByOE[yPEc,OjRyMEi2Nlg9N2F-
CNE1 NIrqSG5CeG:ydH;zbZMhSXO|YYm= NV:3bmlJOjEQvF2= M3;2blQ5cA>? Mn3WZoxw[2u|IFzLMWEucW6mdXPl[EBieG:ydH;zbZM> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl6NUCyPUc,OjN7OEWwNlk9N2F-
CNE2 M1jpR2Fxd3C2b4Ppd{BCe3OjeR?= NFPSeVEzOM7:TR?= NITRfos1QGh? NXL4T3pM[myxY3vzJGxMNUFvaX7keYNm\CCjcH;weI9{cXN? NXXuZ4dkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PFUxOjlpPkKzPVg2ODJ7PD;hQi=>
HL-60 MlWyRZBweHSxc3nzJGF{e2G7 NXSzR2pHPTEEtV2= MWKxbC=> MVjEUXNQ NVPacYhicW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliQlGxOFU> NXHvVGRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFg4PTFpPkKzPVQ5PzVzPD;hQi=>
L929 M2DiXGFxd3C2b4Ppd{BCe3OjeR?= NYjBWWNvOS5{NfMAl|XDqM7:TdMg MVWyOIg> NVOzUFVq[XWpbXXueJMhXE6IzsGtbY5lfWOnZDDu[YNzd3C2b4Ppd{BidmRiYYX0c5Bp[We7 MlzZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NEG3OlkoRjJ|OUSxO|Y6RC:jPh?=
Jurkat MXvD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NXXSOXpwOs7:TR?= M1Ho[VRp NXrtWIp2emW4ZYLz[ZMh\3Kxd4ToJIlvcGmkaYTpc44h[W6mIN8yMYNifGWwaX6g[IVkemWjc3WgZpkhUFNvQWPB MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh7NkC2NUc,OjN6OU[wOlE9N2F-
RAW264.7  MnTWRZBweHSxc3nzJGF{e2G7 NVjabJhtOTBizszN MYmyOIg> NEXuTFRl\WO{ZXHz[ZMhfGinIHHwc5B1d3OrczDpcoR2[2WmIHL5JGdC MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEKwN{c,OjN6MkCyNFM9N2F-
A431  NEXQO4pMcW6jc3WgRZN{[Xl? MWe0NO69VQ>? NUTQTok2Omh? NX3XZZF3[myxY3vzJIZqe2W2aX6tbY5lfWOnZDDjcIVifmGpZTDv[kBk[XOyYYPld{BidmRiUFHSVC=> M3GzcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OECwNFU5Lz5{M{iwNFA2QDxxYU6=
Jurkat MUfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHfoNXIzPS1zMEFOwG0> NIL3fZY3cA>? NX\3Too3cW6qaXLpeJMhgi2IQT3DUWsucW6mdXPl[EBk\WyuIHTlZZRp MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|M{WzNkc,OjN7M{O1N|I9N2F-
COS-7  NXLX[YdnTnWwY4Tpc44hSXO|YYm= NYjE[2hsPTEEtV2= MlW4NlRp NX;DN45Y[W[oZXP0d{B1cGVicILvZ4V{e2mwZzDv[kBCXE5zczD3bZRpKHCxbInRJJRz[WO2cx?= MkPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{OyNFgoRjJ|OUOzNlA5RC:jPh?=
CAL27 NUSxd2ZISXCxcITvd4l{KEG|c3H5 MkTHNVAh|ryP MnfUNog> NGezXpZ{fXCycnXzd4V{KEO3cj3OVJMuemWmdXPl[EB3cWGkaXzpeJkh[nlidYCgeI8hQTBn MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlzN{O5Okc,OjN7MUezPVY9N2F-
THP-1  MmHIRZBweHSxc3nzJGF{e2G7 M4rPflExNTVyzszN NHzBcmczcA>? MUjpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUB1emmydH;sbYRm NF\rOWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwNFI6QSd-MkO5NFAzQTl:L3G+
K562 MnLCRZBweHSxc3nzJGF{e2G7 Mnv1NlDPxE1? NUTQcHBSPDiq NWC5e2NwcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliND3NWS=> NH\L[4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3OlgzPid-MkO4O|Y5OjZ:L3G+
HL-60 NUHROXBwSXCxcITvd4l{KEG|c3H5 MnfoNVAx|ryP NGrk[G0zPGh? MWjpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBi[mmndHHu[UBlcXSncoDlcoV{yqB? NWfScGFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OlU4PzhpPkKzPFY2Pzd6PD;hQi=>
HeLa  MnLMRZBweHSxc3nzJGF{e2G7 NWXaXFJJOjEQvF2= MXiybC=> NELBS2tqdmirYnn0d{BFVU2SIHnu[JVk\WRiYYDvdJRwe2m| MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4M{m2Okc,OjN6NkO5OlY9N2F-
U251 NV;WZowySXCxcITvd4l{KEG|c3H5 NEH4cVUzOM7:TR?= NHvjVGUzPGh? NHvwS3pqdmirYnn0d{Bk[XO2aXPpckBqdmS3Y3XkJGczN01icHjhd4Uh[XK{ZYP0JIFv\CCjcH;weI9{cXN? NVLm[2V{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NVY5OTZpPkKzPFE3QDF4PD;hQi=>
HL-60 MXnBdI9xfG:|aYOgRZN{[Xl? MlXkOVDDvU1? MWW0bC=> NUXRTGVv[myxY3vzJJRp\SClbHXheoFo\SCxZjDjZZNx[XOnLUOsJE06NCCjbnSgVGFTWCCrbnT1Z4VlKGK7IE\z NXXKfIV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NFQ4ODZpPkKzPFA1PzB4PD;hQi=>
Ec-109 NVjx[WkySXCxcITvd4l{KEG|c3H5 MlLnNVAh|ryP NHLkRpc1QGh? NX;BeYRGemWycnXzd4V{KFCHLX3l[IlifGWmIFXjMVExQSClZXzsJIFxd3C2b4Ppdy=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6Mk[0NUc,OjN5OEK2OFE9N2F-
RKO NGrS[4NE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MXOxNEDPxE1? M{DVNFI1cA>? NI\YV4hqdmirYnn0d{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliY3H1d4VlKGK7IFTBRi=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd3OEC2OEc,OjN5NUiwOlQ9N2F-
T98G M37JdWNmdGxiVnnhZoltcXS7IFHzd4F6 NFnTTnUyNTFyMN88US=> M{XFb|I1cA>? MoHabY1xem:4ZYOgZ4VtdCC4aXHibYxqfHliY3;0doVifG2nboSge4l1cCCWUx?= MkPoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NkmyO|UoRjJ|N{[5Nlc2RC:jPh?=
Jurkat NYXpVZQ2S2WubDDWbYFjcWyrdImgRZN{[Xl? M1nETVExOC1{MEFOwG0> NUHscpV{OjSq Mm\SbY5pcWKrdIOgTIFCPCCrbnT1Z4V{KGGyb4D0c5NqeyCmZYDlcoRmdnRib3[gZ49v[2WwdILheIlwdsLi NWLIU4wxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3N|I1QDFpPkKzO|MzPDhzPD;hQi=>
Molt-3  NUHSVGZOSXCxcITvd4l{KEG|c3H5 NEnPV5M2OM7:TR?= NHnVe5czcA>? NUHpZlJQemWmdXPld{Bu\WyjdH;ubY4ucW6mdXPl[EBieG:ydH;zbZM> M4SxVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{K1NFE{Lz5{M{eyOVAyOzxxYU6=
hMSC12 NInpOIlCeG:ydH;zbZMhSXO|YYm= M1e0N|ExOM7:TR?= MUi0[C=> NYS4VHlJcW6qaXLpeJMhd3O2ZX;n[Y5q[yCldXz0eZJmNWmwZIXj[YQh[2WubDDk[YF1cCCjbnSgZ4Ft[2moaXPheIlwdg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ3N{iyNkc,OjN4NUe4NlI9N2F-
HM7  MnLJRZBweHSxc3nzJGF{e2G7 MnywNlDPxE4EoB?= M4f1VlFp M121WIJtd2OtczDhdIlkfWyjcnXuJGEh[WOndHH0[U1qdmS3Y3XkJINie3Cjc3WtN{Bi[3SrdnH0bY9vKGGwZDDQRXJRKGOuZXH2ZYdm MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV6M{SxNkc,OjN3OEO0NVI9N2F-
Hep-2  MX7BdI9xfG:|aYOgRZN{[Xl? MVWyNO69VcLi M2fDb|I1cA>? MYfEUXNQ NF23dlJidGyndnnheIV{KHSqZTDk[YNz\WG|ZTDv[kBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JJNqdGmkaX7pci=> M2G0WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUiwNFMzLz5{M{W4NFA{OjxxYU6=
HSCs MV\BdI9xfG:|aYOgRZN{[Xl? NV7wdoFFPTEQvF2= NH;jclczPGh? M1nZO2ROW09? NXHwfVdCcW6qaXLpeJMhdmmub4TpcoljNWmwZIXj[YQhTE6DIHThcYFo\SCrbnTpZ4F1\WRiYomgVGFTWCClbHXheoFo\Q>? M4fSS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm5PFc1Lz5{M{S5PVg4PDxxYU6=
HL-60  Mm\rR5l1d3SxeHnjbZR6KEG|c3H5 M3X0VFIx|ryPwrC= MUOyOIg> M3LxepJm\HWlZYOgeIhmKGO7dH;0c5hq[yCnZn\lZ5Qhd2ZiRYL5OS=> NX\UTXpiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVQ1QDBpPkKzOFk1PDhyPD;hQi=>
HA Mle2RZBweHSxc3nzJGF{e2G7 NEjHeZo2OM7:TR?= MWKyOIg> Mn3ld5VxeHKnc4Pld{B1cGViY3zlZZZi\2Vib3[gVGFTWCCjbnSgZ4F{eGG|ZT2zMEAuPyxiYX7kJE06KGmwZIXj[YQh[nliYn;yeIV7d22rYj;TRWhC NGrGbIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S3OVk2Pid-MkO0O|U6PTZ:L3G+
C666-1 MWnBdI9xfG:|aYOgRZN{[Xl? MkTKOVDPxE1? MV[yOIg> NIniZml{fXCycnXzd4V{KHSqZTDjcIVifmGpZTDv[kBRSVKSIHHu[EBk[XOyYYPlMVMtKC15LDDhcoQhNTliaX7keYNm\CCkeTDic5J1\XqxbXniM3NCUEF? NYf3Wos4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0O|U6PTZpPkKzOFc2QTV4PD;hQi=>
Hepa1-6 MYLBdI9xfG:|aYOgRZN{[Xl? NGWwZ5E2OM7:TR?= M{nmXVJp MmjnbY5pcWKrdIOgZ5Vz[3WvaX6gZY5lKHKnc4\ldoF1em:uLXnu[JVk\WRiYYDvdJRwe2m| MliwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NE[3OVMoRjJ|NES2O|U{RC:jPh?=
PLC/PRF/5c NHz1UGJCeG:ydH;zbZMhSXO|YYm= MYG1NO69VQ>? Mkf3NYg> M{DHZZBz\X[nboTzJIFxd3C2b4Ppd{B1emmpZ3Xy[YQh[nliQ1nO NVvMb2xDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0N|g5OjRpPkKzOFM5QDJ2PD;hQi=>
HCT116 MXTBdI9xfG:|aYOgRZN{[Xl? Mo[zNVAx|ryP NEXBZ2EyNjWq MXrpcohq[mm2czDPd{1qdmS3Y3XkJINmdGxiZHXheIg> MkHkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NESzOVMoRjJ|N{S0N|U{RC:jPh?=
HCEC MlS5SpVv[3Srb36gRZN{[Xl? MWe1NO69VQ>? M{fVe|czcA>? NEKycnhFVVOR M1WwcZJme3SxcnXzJI9nKHSqZTDuc5Ju[WxiSFPFR{BxcGWwb4T5dIU> NEK1RnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e0NlAyOSd-MkO3OFIxOTF:L3G+
Primary rat cerebral cortical neurons NG\4Zm5CeG:ydH;zbZMhSXO|YYm= MV:xNFDPxE1? NYf5cG5xOWh? MWjwdoV3\W62czDD[E1qdmS3Y3XkJIFxd3C2b4Ppd{BidmRiY3XscEBl\WG2aB?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2MUOxO{c,OjN5NEGzNVc9N2F-
MDA-MB-231  MmHRRZBweHSxc3nzJGF{e2G7 M2P0NlI2|ryP M{XuTVI1cA>? MlnhZYJzd2ejdHXzJIN6fG:2b4jpZ4l1gSCjbnSgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[U0{KGGwZDDQRXJRKGmwZIXj[YQh[nliUnez MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN|N{W0OEc,OjV|M{e1OFQ9N2F-
NLRP3-Tet-on-MC/9  NGrUcIZCeG:ydH;zbZMhSXO|YYm= MnzwNVAuPDEQvF2= MXSxNog> MlX2ZZR1\W63YYTld{B1cGViY3XscEBl\WG2aDDjZZV{\WRiYomgR2FRWy2jc4PvZ4lifGWmIF7MVnA{KG23dHHueJMhcW5idHjlJHRmfC2xbjDzfZN1\W1? NXjwdpJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFM{QDlpPkKzO|A{Ozh7PD;hQi=>
KNS42  MV\BdI9xfG:|aYOgRZN{[Xl? NVO5UJduPTEQvF2= Mo\JNYg> NV32c3VJemWmdXPld{Bk\WyuIHTlZZRpKGGwZDDjc41xdGW2ZXz5JIFjd2yrc3jl[EBk[XOyYYPlJFMwPyCjY4Tpeol1gSCrbjDy[ZNxd26|ZTD0c{BCSlRvMk[zM|JFTy:vZYTmc5JucW5iY3;tZolv[XSrb36= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ7MUG0OUc,OjN4OUGxOFU9N2F-
MCF-7  MWHBdI9xfG:|aYOgRZN{[Xl? NWLt[Jl[OjEQvF5CpC=> M{K1dFczcA>? M{juSIlvcGmkaYTzJIVyfW:uLTDhcoQhPC2RSGStcYVlcWG2ZXSgZZBweHSxc3nz Mn:zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{W2OFMoRjJ|Nke1OlQ{RC:jPh?=
hCMEC/D3  MX\BdI9xfG:|aYOgRZN{[Xl? NGjMU4szPc7:TR?= NYXyXHdTPzKq NF24W5Bz\WS3Y3XzJGx1gEFiaX7keYNm\CCjcH;weI9{cXN? NIewe2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[2OVE6QCd-MkO2OlUyQTh:L3G+
Jurkat NI\sU25CeG:ydH;zbZMhSXO|YYm= M16yZ|EzNjVvNUFOwG0> NYjh[3hLOWh? Mnvs[I9{\SCmZYDlcoRmdnSueTDzeZBxemW|c3XzJHNRUC2rbnT1Z4VlKFCjcj20JINt\WG4YXflMEBRSVKSIHPs[YF3[WenLDDEUmEh\nKjZ33lcpRifGmxbjygZY5lKGyxc4Ogc4YhfmmjYnnsbZR6 MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2Mkm3Okc,OjN2NEK5O|Y9N2F-
CNE-1 M3vwfmFxd3C2b4Ppd{BCe3OjeR?= NHTkZ|kzOM7:TdMg NXnvSYV2OjSq M4LvR4lvcGmkaYTzJHJCTDByMT3pcoR2[2WmIHPlcIwh\GWjdHi= MlfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2Mk[4OVAoRjJ|NEK2PFUxRC:jPh?=
HONE-1 NE\uUWdCeG:ydH;zbZMhSXO|YYm= MVOyNO69VcLi Mo\xNlRp NWrOTFVIcW6qaXLpeJMhWkGGMECxMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NHvUVIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SyOlg2OCd-MkO0NlY5PTB:L3G+
astrocytes cell MXrBdI9xfG:|aYOgRZN{[Xl? MlnIOFDPxE1? MoTQOog> MWXy[YR2[2W|IHXhdox6KGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIP0ZZVzd3Oyb4LpcoU> M2[wRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEGxO|c5Lz5{M{SxNVc4QDxxYU6=
U-937 NXjXOYh4SXCxcITvd4l{KEG|c3H5 NHTUbFEyOM7:TR?= MonJN|BucW5? Ml[4dJJmfmWwdIOgWG5HNWmwZIXj[YQhdmWlcn;weI9{cXN? NUK2NpRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVA4PDhpPkKzOFExPzR6PD;hQi=>
MDA-MB-231 Mo[5RZBweHSxc3nzJGF{e2G7 M3;KTVExOM7:TR?= NHTJbWwycA>? M4rHe4lvcGmkaYTzJJNmdnOrdHn6ZZRqd25idH:gWHJCUUxidYDvckBOXESKIHTve44uemWpdXzheIlwdg>? NWLLTZlKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFg1OjlpPkKzOFA5PDJ7PD;hQi=>
HeLa  MnTKRZBweHSxc3nzJGF{e2G7 M1PyNlExOM7:TR?= NWPuOVFnOmh? MmrXZoxw[2u|IFrSV{0yPSCLbnT1Z4V{KEGyb4D0c5Rq[yCFZXzsJGRm[XSq M3iyWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{S0NFQ2Lz5{M{O0OFA1PTxxYU6=
Ec109 MXfBdI9xfG:|aYOgRZN{[Xl? MoHSNVDPxE1? NGe5OJU3cA>? M{TUZYJtd2OtczDhdI9xfG:2aXOgZ49u[mmwYYTpc44hd2ZiVHH0MXNu[WOQNzDhcoQhemGmaXH0bY9v MlXvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|M{i1OlgoRjJ|M{O4OVY5RC:jPh?=
H460  NUDWfpdNSXCxcITvd4l{KEG|c3H5 M{C3SVEx|ryP NV7SSpo1Pmh? M4XBVYJtd2OtczDhdI9xfG:2aXOgZ49u[mmwYYTpc44hd2ZiVHH0MXNu[WOQNzDhcoQhemGmaXH0bY9v M{PDZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{O4OVY5Lz5{M{OzPFU3QDxxYU6=
HeLa  MXrBdI9xfG:|aYOgRZN{[Xl? MVi1NO69VQ>? MV2xMlVp MkX0ZYJzd2ejdHXzxsBEcGyjbYnkbYEucW6mdXPl[EBieG:ydH;zbZM> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNyM{iwOEc,OjN|MEO4NFQ9N2F-
SK-HEP1 M3;1eGFxd3C2b4Ppd{BCe3OjeR?= NVTDPWkyOTByzszN M2PjVlFp NIXCOGlqdmirYnn0d{BEelRzIHHjeIl3[XSnZDDjZZNx[XOnLUOsJE04NCBvODygMVktKGGwZDDwc4x6MEGGUD3ybYJwe2VrIIDvcJlu\XKjc3W= M4fOPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{CyOlUxLz5{M{OwNlY2ODxxYU6=
QGY7701 MmrURZBweHSxc3nzJGF{e2G7 MlXONlXPxE1? M2\nWFEvPWh? MoP3bY5pcWKrdIOgZYNkfW23bHH0bY9vKG:oIIP1Zk1IOSCyaHHz[UBqdmS3Y3XkJIJ6KESRWDCrJJF2\XKlZYTpci=> MnTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NECwOlEoRjJ|MkSwNFYyRC:jPh?=
HepG2 M1;yPGFxd3C2b4Ppd{BCe3OjeR?= M3nPXFIx|ryP NVKzOWc3OzCvaX6= M1TGc4lvcGmkaYTzJJRp\SCnbnjhcoNm\CClZXzsJIRm[XSqIHL5JINwdWKrbnXkJJRz\WG2bXXueEBw\iCjcHnn[Y5qdiCjbnSgWHJCUUx? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{NEKzPUc,OjN{MkSyN|k9N2F-
U87  NYHFRox6SXCxcITvd4l{KEG|c3H5 MkXnNlXPxE1? NFrjcGQzcA>? NGq0XG1l\WO{ZXHz[ZMhcXOxbHnxeYlzcXSrZ3XubY4hMEmVTDmtbY5lfWOnZDDhdI9xfG:2aXOgZ4VtdCCmZXH0bEwh[nW2IH7veEBv\WO{b4TpZ{Bk\WyuIHTlZZRp MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{OU[yOkc,OjN{Mkm2NlY9N2F-
HSC-2 MmnnRZBweHSxc3nzJGF{e2G7 MnKyNlUwPTEQvF2= M13tNlFp MYPpcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?= Ml7GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNUWyOFgoRjJ|MUW1NlQ5RC:jPh?=
HSC-4 MYDBdI9xfG:|aYOgRZN{[Xl? MkjENlUwPTEQvF2= NE[3[2IycA>? MmjkbY5pcWKrdIOgV24uOzhvaX7keYNm\CCleYTveI95cWOrdIm= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF3NUK0PEc,OjNzNUWyOFg9N2F-
CL-1 NUDHXmJlSXCxcITvd4l{KEG|c3H5 MoPuNlDPxE1? MUCxbC=> MUPicI9kc3NiT2C0OFkhcW6mdXPl[EBk\WyuIHTlZZRp M4\oOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUOxO|gzLz5{M{GzNVc5OjxxYU6=
MEL MV;BdI9xfG:|aYOgRZN{[Xl? NHK4cGYzOM7:TR?= MXSyOIg> Ml:4SG1UVw>? NWPOUYE6cW2yYXny[ZMhWDK[Nz3pcoR2[2WmIF3FUEBk\WyuIHHwc5B1d3Orcx?= M2riSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEG0PFg4Lz5{M{CxOFg5PzxxYU6=
Bel-7402  MWTDZZNx[XOnIFHjeIl3[XSrb36gRZN{[Xl? MYS1NO69VQ>? MUiybC=> NYnkZox5cW6qaXLpeJMhWEOHLXnu[JVk\WRiYX7vbYtqew>? M3qwUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEC4O|QzLz5{M{CwPFc1OjxxYU6=
Eca-109 M3jLcWFxd3C2b4Ppd{BCe3OjeR?= MmSwNlXPxE1? M331OVMxdWmw MVnpcohq[mm2czDCTk1DOTFvaX7keYNm\CClZXzsJIRm[XSq NXjTOplKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|Y6PjdpPkKzNFc3QTZ5PD;hQi=>
MEL NFu5bFhCeG:ydH;zbZMhSXO|YYm= M3r5WlIx|ryP M2XuUFFp NGrHb5dFVVOR NYO3e2N[cW2yYXny[ZMhWDK[Nz3pcoR2[2WmIF3FUEBk\WyuIHHwc5B1d3Orcx?= NGnqWWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxOFg5Pyd-MkOwNVQ5QDd:L3G+
Bel-7402  NH\FcW1CeG:ydH;zbZMhSXO|YYm= NY[1UplCPTEQvF2= M{WzdVJp MlfCbY5pcWKrdIOgVGNGNWmwZIXj[YQh[W6xaXvpdy=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzByOEe0Nkc,OjNyMEi3OFI9N2F-
L929 Ml:3SpVv[3Srb36gRZN{[Xl? MmLaNk42|ryP NELmN3gycA>? NFnhU|dqdmO{ZXHz[ZMhWkmSMTDlfJBz\XO|aX;uJIFv\CCneHHj[ZJj[XSnZDDUUmbPuS2rbnT1Z4VlKG2rdH;jbI9v\HKrYXyg[Jl{\nWwY4Tpc44h[W6mIGLPV{Bxem:mdXP0bY9v Mn3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEC1NVgoRjJ|MECwOVE5RC:jPh?=
RCC  NVmxTmlMS2WubDDWbYFjcWyrdImgRZN{[Xl? NGrOd|QyODEQvF2= MoXMNlRp M1rjbJJm[2:4ZYLld{B1cGVidnnhZoltcXS7IH;mJINmdGy|IHX4dI9{\WRidH:gNVVlNVCJSkK= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl7MUS5OEc,OjJ7OUG0PVQ9N2F-
NB2a/d1 MmPMRZBweHSxc3nzJGF{e2G7 MmTWNVAx|ryP M3\KblczcA>? Ml7hZZR1\W63YYTld{B{fGG3cn;zdI9zcW6nLXnu[JVk\WRiY3HzdIF{\SCjY4Tpeol1gSxiUFHSVEwh[W6mIITheUBkdGWjdnHn[S=> M4nxWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUi4OVQyLz5{Mkm4PFU1OTxxYU6=
T cell NV;ZO2ZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV2yOU0yODEQvF2= MXiyOIg> MoGx[I9{\S2mZYDlcoRmdnSueTDpcohq[mm2ZXSgWEBk\WyuIIDyc4xq\mW{YYTpc44hdWWmaXH0[YQhfGi{b4XnbEB1cGViY3:td5RqdXWuYYTpc44hf2m2aDDhcpRqNUOGMzDhcoQh[W62aT3DSFI5 NH6wTlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm4NlU{QCd-MkK5PFI2Ozh:L3G+
K562 MV7BdI9xfG:|aYOgRZN{[Xl? NV\1bY1EOTByzszN MUCxbC=> NEmxUoxjdG:la4OgRYJvd2KjdnnzZ5VuyqCILXnu[JVk\WRiYYDvdJRwe2m| NFjNOZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm3NlM4Oid-MkK5O|I{PzJ:L3G+
Jurkat  NIm2VXFCeG:ydH;zbZMhSXO|YYm= MkHtOFDPxE1? NVjwNYhiOWh? M3HlWYFjd2yrc3jld{BH[XOOLXnu[JVk\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRp M2jt[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyO|M5Lz5{Mkm0Nlc{QDxxYU6=
BGC-823 M4TxNWFxd3C2b4Ppd{BCe3OjeR?= NUPUbHZvOTByzszN MmTyNYg> NVTybHhReGG{dHnhcIx6KHKnc3P1[ZMh[2WubIOgZYdicW6|dDDkZY1i\2Vib3[g[IFq\HqnaX6= NGrvWHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlU1PSd-MkK5NlY2PDV:L3G+
Hep3B M{L2dWFxd3C2b4Ppd{BCe3OjeR?= NVnhRZpCPTEQvF2= M3O2NlFp MnO4Zoxw[2u|IHHwc5B1d3OrczDpcoR2[2WmIHL5JGhGT0O| NIe5eHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyN|E2PCd-MkK5NlMyPTR:L3G+
LLC-PK1 M2nQVmFxd3C2b4Ppd{BCe3OjeR?= NF;WSVUzOM7:TR?= MnrCNYg> Mn;PdJJmfmWwdIOgZ4l{eGyjdHnuMYlv\HWlZXSg[IVoemGmYYTpc44hd2ZiQYTnOUwh[mWlbHnuMVEtKGGwZDDBeIcyOiCycn;0[Ylvew>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh7NkCzO{c,OjJ6OU[wN|c9N2F-
A549 NHnUVXBCeG:ydH;zbZMhSXO|YYm= MVi1NO69VQ>? NEjOc2kycA>? M2\hVYJtd2OtczD0bIUhSkGLLXnu[JVk\WRiYYDvdJRwe2m|wrC= NUTU[I5yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4PFczOTVpPkKyPFg4OjF3PD;hQi=>
SGC-7901  NGrpNllCeG:ydH;zbZMhSXO|YYm= M2L5ZlEx|ryP MUGyOIg> NVLsSZpjcW6qaXLpeJMh|rJuzsKt[Ilu\XSqeXzhZ5J6dHOqaXvvcolvNWmwZIXj[YQh[XCxcITvd4l{yqB? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh2OEW5O{c,OjJ6NEi1PVc9N2F-
DM6 M4POcWFxd3C2b4Ppd{BCe3OjeR?= NX3Mc3FnOTByzszN MnW3O|Jp NGrSdmRjdG:la4OgZo91cCCHMl[tNUBidmRiRULGeJIudWWmaXH0[YQh[3m2b4TvfIlkcXS7 MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NUOyPEc,OjJ6MkWzNlg9N2F-
MCF-7, MDA-MB-468, Caco-2 M{jrSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYK1NO69VQ>? NVfHXINzPDiq NEHVXpNqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBqdmS3Y3XkJIJ6KHOjcH;ubY4> M3TKNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OECwPVY5Lz5{MkiwNFk3QDxxYU6=
A2750  NUnKc29NSXCxcITvd4l{KEG|c3H5 MWCyNO69VQ>? MnftNog> MYrEUXNQ NGHkT|djdG:la4OgZ4F{eGG|ZTDjcIVifmGpZTDkeZJqdmdiaHXs[Y5idGmwIITy[YF1dWWwdDDhcoQhemWmdXPld{BifXSxcHjh[4lkKGOnbHyg[IVifGh? Mn7EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5OESzOlMoRjJ{N{i0N|Y{RC:jPh?=
U87  M2m0VWFxd3C2b4Ppd{BCe3OjeR?= MUKyNO69VQ>? NF;FRYIzPGh? Mmj0doVlfWOnczD0bIUh[XCxcITvd4l{KHKjdHWgbY5lfWOnZDDifUBRVEGE NYL5bZpURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3O|g4QDBpPkKyO|c5PzhyPD;hQi=>
HT1080 NEHOTXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfNRVM2OM7:TR?= M{jnSldl NFXTNnVqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBk[XW|ZXSgZpkh[2:vYnnu[YQhfHKnYYTt[Y51KG:oIFTDRUBidmRiT13Q Mn64QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEC5PFQoRjJ{N{SwPVg1RC:jPh?=
A549  MUPBdI9xfG:|aYOgRZN{[Xl? MoP5OVDPxE1? MYqybC=> NEW1[ZFx[XK2aXHscJkh\GWlcnXhd4V{KHOxZHn1cUB{\Wynbnn0[U1qdmS3Y3XkJIFxd3C2b4Ppdy=> NVP0S|l{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NlE5ODRpPkKyO|IyQDB2PD;hQi=>
Primary OPC MV3BdI9xfG:|aYOgRZN{[Xl? NXy0OI94Oc7:TR?= Ml71OogwOjSq NUHFXVZGemWmdXPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBm[XKueT2gZY5lKGyjdHWtZZBweHSxc3nzM45m[3Kxc3nz M1HLWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{C3N|g2Lz5{MkewO|M5PTxxYU6=
PMNs NH\OSXpCeG:ydH;zbZMhSXO|YYm= NWPZfYZRPDEQvF2= M1XONlZp MYDEUXNQ MVjy[ZZmenOnZDD0bIUh[W2xdX70JI9nKGOuZXH2[YQh[2G|cHHz[U0{KHSxIH7lZZIhfmWqaXPs[UBt\X[nbIO= M3z2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkmyOVc4Lz5{Mk[5NlU4PzxxYU6=
A549  Ml21RZBweHSxc3nzJGF{e2G7 MXy1NO69VQ>? MnvLNYg> MV3wdoV3\W62czDhdI9xfG:|aYOgbY5lfWOnZDDifUBD[W:qdX;zbYRmKEl? NFTUN3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[4O|Y{PSd-MkK2PFc3OzV:L3G+
AGS MU\BdI9xfG:|aYOgRZN{[Xl? M3i4elIx|ryP MlP4NVJp MYLpcohq[mm2czD0bIUh[WO2aY\heIlwdiCxZjDwdo8u[2G|cHHz[U0{KGmwIILld5BwdnOnIITvJJRp\SCHdF;BZ{BnemGldHnvci=> NGrmXI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[4O|M6QCd-MkK2PFc{QTh:L3G+
shC9  M{TxUmFxd3C2b4Ppd{BCe3OjeR?= Mn\ENVDPxE1? NYOxRVkzOT[q MmK2Zox2dnS|IGPITEBmgHC{ZYPzbY9vKGmwIIPoR|kh[2WubIOg[Zhxd3OnZDD0c{BmcXSqZYKgVGEhd3JiTGDD NH7pVWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0NVA6PCd-MkK2OFExQTR:L3G+
primary MEFs MUDGeY5kfGmxbjDBd5NigQ>? M2XyelEzcA>? NX;SeYJ[yqCrbnPy[YF{\XNiZYTvdI9{cWSnLXnu[JVk\WRibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBl\XCxbHHybZpifGmxbh?= NHi3cXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[xN|c3Pyd-MkK2NVM4Pjd:L3G+
3T9 MEFs Mn;1SpVv[3Srb36gRZN{[Xl? NUDLe|JnOT[q NHnnd4JqdmO{ZXHz[ZMh[3m2b3Podo9u\SClIILlcIVie2ViYX\0[ZIh\XSxcH;zbYRmNXS{ZXH0cYVvfA>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzM{e2O{c,OjJ4MUO3Olc9N2F-
3T9 MEFs NYfKWFlXTnWwY4Tpc44hSXO|YYm= NVjaeG1wOTiq M13zfZVxemWpdXzheIV{KGmwaYTpZZRweiClYYPwZZNmNTliYoX0JIRwf26{ZXf1cIF1\XNiZX\m[YN1d3JiY3HzdIF{\XNiYX\0[ZIh\XSxcH;zbYRmKHS{ZXH0cYVvfA>? NFL6eoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[xN|c3Pyd-MkK2NVM4Pjd:L3G+
MDA-MB-231  M2frZWFxd3C2b4Ppd{BCe3OjeR?= Ml7XNlDPxE1? NU\ZNlNzPGR? NF\heFBz\WS3Y3XzJJRp\SClZXzsJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KG2ndH\vdo1qdiC2cnXheI1mdnRid3HzJJNq\26rZnnjZY51dHl? NEDZSZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW5N|Q1OSd-MkK1PVM1PDF:L3G+
C6  MVPBdI9xfG:|aYOgRZN{[Xl? NIOzeVEyOM7:TR?= NVPyc3lsOjSq NIOzbW1jdG:la4OgeIhmKHO3cIDy[ZN{cX[nIHXm[oVkfCCxZjD0bIUheGWydHnk[UBwdiC4aXHibYxqfHl? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6OEm4NEc,OjJ3OEi5PFA9N2F-
HL-60  NWTyPHBFSXCxcITvd4l{KEG|c3H5 MYCxNFDPxE1? NUfZdpFFOjSq M2K4V4lvcGmkaYTzJJRp\SClZXzsJIFxd3C2b4Ppd{B{\WynY4Tl[EApMylvbXXueIh6dCEQsj2oNgKHmjZrLXTp[4x2[2:yeYLhco9{cWSnwrC1 NIixOXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0OlY3QSd-MkK1OFY3Pjl:L3G+
HL-60 Ml;oRZBweHSxc3nzJGF{e2G7 NHLofmkyOC16MN88US=> MoThOIg> MULpcohq[mm2czDUS2hSNWmwZIXj[YQh[2WubDDhdI9xfG:|aYRCpC=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV{M{KyPUc,OjJ3MkOyNlk9N2F-
BCC M{XibmFxd3C2b4Ppd{BCe3OjeR?= NVHPTGNEPTEQvF2= MkHwNYg> NIjNdYxqdmirYnn0d{BFSVSVLX3l[IlifGWmIHfyc5d1cCCrbnjpZol1cW:w M2PZSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUG5OFM3Lz5{MkWxPVQ{PjxxYU6=
RAW 264.7 NVvITYY1SXCxcITvd4l{KEG|c3H5 M1m3RVUxNzFyMN88US=> MVWxbC=> Ml:zZ49v[2WwdILheIlwdi2mZYDlcoRmdnRiaX7obYJqfHNiRF;OMYlv\HWlZXSgdnJPSSClbHXheoFo\Q>? Mn;BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OUG0NlYoRjJ{NEmxOFI3RC:jPh?=
K562 MkDqRZBweHSxc3nzJGF{e2G7 MkHrNlXPxE1? MkjSNog> M1zvS5Bz\X[nboTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGOxLYTy[YF1dWWwdDD3bZRpKGGvdYLlcpNqdiCJIHHu[EBVWkGLTB?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6M{e3O{c,OjJ2OEO3O|c9N2F-
SGC-7901 NYfq[3E5SXCxcITvd4l{KEG|c3H5 M{\IN|IxKM7:TdMg M3TaelJp MWrheJRmdnWjdHXzJGgzVzMEoH;yJHRPTiEQsT3pcoR2[2WmIHPlcIwh[XCxcITvd4l{KGG|IIflcIwh[XNiY3HzdIF{\S1|IHHjeIl3cXS7wrC= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR5MUW4PUc,OjJ2N{G1PFk9N2F-
PC3 MkPzRZBweHSxc3nzJGF{e2G7 NInvNlEyOM7:TR?= MVy0bC=> NHn4d3lkd3WwdHXyd{BndGG4b3PvfIllNWmwZIXj[YQh[2G|cHHz[U1z\WyjdHXkJIFxd3C2b4Ppdy=> MkLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2N{G5O|QoRjJ{NEexPVc1RC:jPh?=
SMMC-7721 NVr2VGFxSXCxcITvd4l{KEG|c3H5 NUjGfXNYPTEQvF2= M{HiT|Q5cA>? MVLheJRmdnWjdHXzJI9VWi2rbnT1Z4VlKGGyb4D0c5Nqew>? NF7GPIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS2OVg{Oyd-MkK0OlU5OzN:L3G+
HeLa  MkHDRZBweHSxc3nzJGF{e2G7 NXHj[FhVPTEEoN88US=> NYfPOFdCPC96aB?= MYrpcohq[mm2czDTWHMucW6mdXPl[EBt[XSnLYDoZZNmKGGyb4D0c5Rq[yCndnXueJM> NGPQN4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS2NFUxPCd-MkK0OlA2ODR:L3G+
HeLa  NFfxXZBCeG:ydH;zbZMhSXO|YYm= NWXv[YxtPTEEoN88US=> NIrJNXEycA>? MUXzeZBxemW|c3XzJJRp\SCIUlHQMYlv\HWlZXSgZYNkfW23bHH0bY9vKG:oIHHucoV5cW5iVjDwc5NqfGm4ZTDj[Yxtew>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR2OUS0NEc,OjJ2NEm0OFA9N2F-
T47D  NUL4bYJtSXCxcITvd4l{KEG|c3H5 NWPzZYZxOTByzszN MXSxbC=> MXPicI9kc3NidHjlJIdmdmW{YYTpc44hd2ZiRT3jZYQwS1SIMjDifUBUXFN? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjRyMUG2PEc,OjJ2MEGxOlg9N2F-
HeLa  NFnGfYxCeG:ydH;zbZMhSXO|YYm= M4fjb|Mx|ryP Mn3SOIg> M2ixeOKhcW6lcnXhd4V{KHSqZTDn[Y5memGuIHPlcIwhfmmjYnnsbZR6KDR6IHigZYZ1\XJicHjveI9lgW6jbXnjJJRz\WG2bXXueOKh MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN7NEK0PEc,OjJ|OUSyOFg9N2F-
HCC M4W0O2Fxd3C2b4Ppd{BCe3OjeR?= NHLZfVgzOCEQvF5CpC=> MXGybC=> NGnkNZJifHSnboXheIV{KHSqZTDETGEhcW6mdXPl[EBi[3SrdnH0bY9vKG:oIGDBVnA> NXL2Vmd1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOFI4OzJpPkKyN|QzPzN{PD;hQi=>
mESCs NH\ZSoJCeG:ydH;zbZMhSXO|YYm= M2CwRVIvPc7:TR?= NInC[okzcA>? NH\neXRqdmirYnn0d{B1cGViTnHGMY1m\GmjdHXkJINie3Cjc3WgZYN1cX[jdHnvci=> M2PoZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mki1Nlc1Lz5{MkK4OVI4PDxxYU6=
EMT-6  MVzDfZRwfG:6aXPpeJkhSXO|YYm= NH64U3MyODEQvF2= M{fKRlFp NF7ZUo9x[XK2aXHscJkh[myxY3vl[EBk\WyuIHTlZZRpKGmwZIXj[YQh[nlic3nyZY1me2mwZR?= MoXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NUG5NlEoRjJ{MkWxPVIyRC:jPh?=
MCF7 MlzkRZBweHSxc3nzJGF{e2G7 MWS1NOKh|ryP Ml3nNYg> M16w[4lvcGmkaYTzJHBCKGGldHn2ZZRm\CClYYPwZZNmNTNuID25MEBidmRicH;sfUhCTFBvcnnic5NmMSCyb3z5cYVz[XOn NXfHbGtVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlM{PDVpPkKyNlI{OzR3PD;hQi=>
K562 M3mzdmFxd3C2b4Ppd{BCe3OjeR?= M{DScFIxKM7:TdMg MkjxOFhp MlfjSG1UVw>? NHrVT3hjdG:la4OgcIFx[XSrbnniMYlv\HWlZXSgbY5pcWKrdHnvckBw\iC4aXHibYxqfHliYX7kJIFxd3C2b4Ppd{BqdmS3Y4Tpc44> NI[3Tog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKxOlE2QCd-MkKyNVYyPTh:L3G+
Molt4-hyg MnraRZBweHSxc3nzJGF{e2G7 NYqyfpJlOTEQvF2= Ml[wNE42cA>? MU\icI9kc3NiZnHycoV{d2xvaX7keYNm\CClYYPwZZNmNTNvbHnr[UBi[3Srdnn0fS=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5NUixNUc,OjZ{N{W4NVE9N2F-
HeLa MnW3RZBweHSxc3nzJGF{e2G7 MVuxNO69VQ>? MnWyNE42cA>? MlfFbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhfGinIHPvcYJqdmWmIITy[YF1dWWwdDD3bZRpKGexbXnzbY4hViCjbnSgWHJCUUx? NEjieXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkG3PVY3OSd-MkKxO|k3PjF:L3G+
Jurkat T NULUZWNSSXCxcITvd4l{KEG|c3H5 M2rKNlMx|ryP NILSemIxNjWq MljuSG1UVw>? MV3icI9kc3NidHjlJJpqemGvLXnu[JVk\WRiYYDvdJRwe2m| NWGzRpRWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxOVk5QThpPkKyNVU6QDl6PD;hQi=>
Neutrophil M4jUZ2Fxd3C2b4Ppd{BCe3OjeR?= Mn;UNlDPxE1? M4Die|AvPWh? NH;rcodifHSnboXheIV{KHSqZTDwdo8u[XCxcITveIlkKGWoZnXjeEBw\iCPYWKx NX;VTXZ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVY5PDRpPkK2NVk3QDR2PD;hQi=>
HCT116 NGnBO|VCeG:ydH;zbZMhSXO|YYm= MnTJOVDDqM7:TR?= NUSxWJVPOmh? M3PpNpJmfmW{c3XzJJN6dmW{Z3nzeIlkKGGyb4D0c5NqeyCnZn\lZ5R{KG:oIHPlcIVkd3irYjDhcoQhVlCFLUG2xsA> NHHse5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkG1PVc2Oid-MkKxOVk4PTJ:L3G+
MDA-MB-231  NVTpPWZwSXCxcITvd4l{KEG|c3H5 NXHS[XhCOi53LUeuOe69VQ>? M3zZPFJp MYXpcohq[mm2czD0bIUh[2WubDDk[YF1cCCxZjDNSGEuVUJvMkOxJINmdGy|IHnu[JVk\WRiYomgV2RVKGmwIHGgZ49v[2WwdILheIlwdiCmZYDlcoRmdnRibXHucoVz M13ZVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUG1OVI3Lz5{MkGxOVUzPjxxYU6=
LNCaP M2\xd2Fxd3C2b4Ppd{BCe3OjeR?= MoHJOFDPxE1? MUmybC=> MVTpcohq[mm2czDieZRmcW5iaX7keYNm\CClZXzsJIFxd3C2b4Ppdy=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzNEe2OEc,OjJzMUS3OlQ9N2F-
MB231 M13nVGFxd3C2b4Ppd{BCe3OjeR?= NYTZOFE3OTByzszN M2nSOlFp MWLEUXNQ Ml;iZYJzd2ejdHXzJJRp\SCrbnT1Z5Rqd25ib3[gZ4VtdCCmZXH0bEBjgSCZRVWxJIlvcGmkaYTpc44tKFSUQVnMJJRz\WG2bXXueEwh[W6mIITo[UBkd22kaX7heIlwdg>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzMkm0NEc,OjJzMUK5OFA9N2F-
HCC38  M37MeGFxd3C2b4Ppd{BCe3OjeR?= MUGxNFDPxE1? MnToNYg> Ml[ySG1UVw>? MWjhZpJw\2G2ZYOgeIhmKGmwZIXjeIlwdiCxZjDj[YxtKGSnYYToJIJ6KFeHRUGgbY5pcWKrdHnvckwhXFKDSVygeJJm[XSvZX70MEBidmRidHjlJINwdWKrbnH0bY9v M3vVflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUGyPVQxLz5{MkGxNlk1ODxxYU6=
MDA-MB231 MkTpRZBweHSxc3nzJGF{e2G7 MV21NOKh|ryP NFjxblEzPGh? M1ruZmROW09? MYfhZpJw\2G2ZYOgZ5Vz[3WvaX6tbY5lfWOnZDDj[YxtKGSnYYTo MojHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMEGzN|UoRjJ{MUCxN|M2RC:jPh?=
LNCaP MUPBdI9xfG:|aYOgRZN{[Xl? NUTGbIZ1PTEEoN88US=> NVPVWJgyOjSq MlnLSG1UVw>? NX[0VWd5[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M37Hb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUCxN|M2Lz5{MkGwNVM{PTxxYU6=
HCT116 NVr6dHZOSXCxcITvd4l{KEG|c3H5 MVy1NOKh|ryP NWPEfJYzOjSq NEPIfHhFVVOR NWDrZ2FM[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M2rY[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUCxN|M2Lz5{MkGwNVM{PTxxYU6=
Ishikawa  MlrGRZBweHSxc3nzJGF{e2G7 M1v4bFI2|ryP MWiyOIg> M1fObpJm\HWlZYOgZ4VtdCCmZXH0bEBidmRiYYDvdJRwe2m|IHnu[JVk\WRiYomgRoFnKEFz NFvRcow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4PFkyQCd-MkKwPFg6OTh:L3G+
 YD-8  M3;zdmFxd3C2b4Ppd{BCe3OjeR?= NFTCSXIyODEQvF2= M2jqRlFp Mlm0bY5pcWKrdIOgVGxGVy2rbnT1Z4VlKGGyb4D0c5Nqew>? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB6NkG4N{c,OjJyOE[xPFM9N2F-
eosinophil  NFLjb|JCeG:ydH;zbZMhSXO|YYm= NXXTPG0{QTEQvF2= Mny5NlRp MXzpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBH[XNiYX70bYJw\HoEoB?= NYC4dnFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwO|k{OzRpPkKyNFc6OzN2PD;hQi=>
L929 NXTaXJFIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm[2Nk42|ryP MVGyOIg> NYmwXmV2cW6qaXLpeJMhfGinIHX4dJJme3Orb36gc4YheC2yM{igZY5lKE6ILd86RkB1dyCjdXft[Y51KFSQRt8xMYlv\HWlZXSgcoVkem:ydH;zbZMh[W6mIHH1eI9xcGGpeR?= MnjJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkewPVcoRjJ{MEK3NFk4RC:jPh?=
YD-8  NEPVbIhCeG:ydH;zbZMhSXO|YYm= MnrBNVAx|ryP NYq4N5VQOWh? MlywZoxw[2u|IITo[UBIWy2KQ3ytbY5lfWOnZDDhdI9xfG:|aYO= M{TXZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKwNFc5Lz5{MkCyNFA4QDxxYU6=
HBx MlnYRZBweHSxc3nzJGF{e2G7 M{W2clI2|ryP MliyOFhp M{HNN2ROW09? MVfy[YR2[2WmIHPlcIwh\GWjdHigbY5lfWOnZDDifUA{NU2D MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{MEC3PEc,OjJyMkCwO|g9N2F-
U937  MWHBdI9xfG:|aYOgRZN{[Xl? NYDaSlg{PTEEoN88US=> NVLzcnVUOWh? NWfxXm97cW6qaXLpeJMhUEZvaX7keYNm\CCjcH;weI9{cXQEoB?= NX\JcItuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5PVg4OzFpPkKxPVk5PzNzPD;hQi=>
HL60 MkGyRZBweHSxc3nzJGF{e2G7 NVXBNnAyPDEQvF2= MUO0NI1qdg>? Mn7kSG1UVw>? Mn;iZoxw[2u|IFLOSGMh[2:vcH;1coR{KGmwZIXj[UBmgHCxc4Xy[UBw\iCyaH;zdIhifGmmeXzz[ZJqdmViYX7kJGRPSSCocnHncYVvfGG2aX;u MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl6M{K5Okc,OjF7OEOyPVY9N2F-
M-14 NH;Fc3dCeG:ydH;zbZMhSXO|YYm= MWCyOe69VQ>? NFvTT2cxNjWq NWTlPFVKyqCrbnjpZol1eyCkb4ToJJRp\SClcoXk[UBmgHS{YXP0MUBidmRiY3;tdI92dmRvaX7keYNm\CCjcH;weI9{cXN? M3jEW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUW0PVU6Lz5{MUm1OFk2QTxxYU6=
SK-BR-3 M{fqWmFxd3C2b4Ppd{BCe3OjeR?= NGPWNJE2OMLizszN NI[3PIMzcA>? M1r6ToJtd2OtczDhdI9xfG:2aXOgSG5CKG[{YXft[Y51[XSrb36gbY5lfWOnZDDifUBkd22kaX7heIlwdiCxZjDTUU0yPjRiYX7kJJJi\GmjdHnvci=> M3vLeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUCxN|g3Lz5{MUmwNVM5PjxxYU6=
MDA-MB-468 M2mwbGFxd3C2b4Ppd{BCe3OjeR?= NEfCbHY2OMLizszN NU\QZlRCOmh? MXHicI9kc3NiYYDvdJRwfGmlIFTORUBnemGpbXXueIF1cW:wIHnu[JVk\WRiYomgZ49u[mmwYYTpc44hd2ZiU12tNVY1KGGwZDDyZYRq[XSrb36= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlyMUO4Okc,OjF7MEGzPFY9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3; 

PubMed: 27191889     


With the addition of Z-VAD-FMK, levels of cleaved Caspase-3 and PARP1 were decreased.

Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II; 

PubMed: 27191889     


Levels of Beclin1, Atg5, Atg7 and LC3-II were decreased after IFN-γ stimulation. However, when IFN-γ was combined with the caspase inhibitor, levels of Beclin1, Atg5, Atg7 and LC3-II were not decreased as determined by the Western Blot test. 

p23 / Hsp90 / hTRET / MMP2; 

PubMed: 19740745     


Expression of Hsp90 client proteins during cell death. HeLa cells were preincubated with z-VAD-fmk (100 μM) for 1 h and exposed to various concentrations of tunicamycin (Tu, in μM) for 24 h. Cell extracts were prepared and analyzed by Western blotting. β-Actin was used as the loading control.

cyto C; 

PubMed: 17301139     


z-VAD-fmk did not inhibit cytochrome c release in RRV-infected MA104 cells (RRV: rhesus rotavirus). Mock- and RRV-infected MA104 cells either were not pretreated or were pretreated with 100 μM z-VAD-fmk for 2 h before RRV infection, and treatment was continued during infection. Fourteen hours after RRV infection, the cytosolic and heavy-membrane fractions were assayed for cytochrome c by Western blotting. Mock-infected MA104 cells were used as negative controls. Cox IV and tubulin were used as protein loading controls for the heavy-membrane and cytosolic fractions, respectively. Positions of molecular weight markers are indicated on the left.

27191889 19740745 17301139
Growth inhibition assay
Cell viability; 

PubMed: 12522090     


Inhibition of magnolol-induced apoptotic cell death by z-VAD-fmk. CH27 cells were treated with cell permeable broad-spectrum caspase inhibitor (z-VAD-fmk) 2 h prior to 80 μm magnolol treatment. Cell viability was estimated. ***P<0.001, compared with the 80 μM magnolol-treated group. 

12522090
Immunofluorescence
cytochrome c ; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Smac/DIABLO; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Endo G; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of EndoG together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

AIF; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of AIF together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

11726499 12941691
ELISA
IL-1β; 

PubMed: 26254306     


Cell-free medium supernatants were harvested at 5 h. IL-1β levels were measured by ELISA. Z-VAD-FMK was used as a positive control. Bars represent means ± sem of pooled results from 3 experiments performed in triplicate at different times.

26254306
In vivo In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo[7].

Protocol

Cell Research:

[6]

- Collapse
  • Cell lines: human granulosa cell lines (GC1a, HGL5, COV434)
  • Concentrations: 50 μM
  • Incubation Time: 48 h
  • Method:

    To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h with etoposide (50 μg/ml) and/or Z-VAD-FMK (50 μM) under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: CD1 mice
  • Dosages: 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (198.93 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+35 %PEG 300+2%Tween 80+ddH2O
For best results, use promptly after mixing.
6mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage powder
in solvent
Synonyms Z-VAD(OH)-FMK
Smiles CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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% DMSO % % Tween 80 % ddH2O
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Can you suggest an in vivo formulation of S7023 for animal studies?

  • Answer:

    For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

  • Question 2:

    What is difference between S7023 & S8102?

  • Answer:

    Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

Caspase Signaling Pathway Map

Related Caspase Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID