Z-VAD-FMK

For research use only.

Catalog No.S7023 Synonyms: Z-VAD(OMe)-FMK

687 publications

Z-VAD-FMK Chemical Structure

CAS No. 187389-52-2

Z-VAD-FMK (Z-VAD(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 849 In stock
USD 117 In stock
USD 470 In stock
USD 1190 In stock

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Z-VAD-FMK has been cited by 687 publications

Purity & Quality Control

Choose Selective Caspase Inhibitors

Biological Activity

Description Z-VAD-FMK (Z-VAD(OMe)-FMK) is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Features A key compound for apoptosis studies.
Targets
Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro

Z-VAD-FMK (10 μM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 μM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U937 NYHpflJMSXCxcITvd4l{KEG|c3H5 MWK1NO69VQ>? M13ZNVQ5cA>? NIXuOJNz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KFONST3JTUB1emWjdH3lcpTDqA>? M37F[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK0NFQ{Lz5{NUiyOFA1OzxxYU6=
HL-60 M4HYS2Fxd3C2b4Ppd{BCe3OjeR?= MU[1NO69VQ>? NH7GcpU1QGh? MkjIdoVlfWOnczDj[YxtKGSnYYToJINifXOnZDDifUBUU0lvSVmgeJJm[XSvZX70xsA> Mn3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkSwOFMoRjJ3OEK0NFQ{RC:jPh?=
DTK-SME NFPrdXRCeG:ydH;zbZMhSXO|YYm= MXe1NO69VQ>? MX[ybC=> M1X3VmROW09? NEXYUnBx[XK2aXHscJkhcW6qaXLpeJMhfGinIHHwc5B1d3OrczDpcoR2[2WmIHL5JIJ2eGm4YXPhbY5m MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh2M{i5O{c,OjV6NEO4PVc9N2F-
HCT116  M3rzfmFxd3C2b4Ppd{BCe3OjeR?= NYDxUmRzPTEQvF2= NVHEd3hbOmh? MmO1bY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDESHZR M3fBTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OE[4PFE5Lz5{NUi2PFgyQDxxYU6=
SGN MV7BdI9xfG:|aYOgRZN{[Xl? MnTiNlBuVQ>? MnjlOFhp MX3oZZMhdm9iaX7mcJVmdmOnIH;uJGFKTixiY3HsdIFqdiCneIDy[ZN{cW:wIH;yJINmdGxiYYDvdJRwe2m| M37jXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEe0OlM{Lz5{NUi3OFY{OzxxYU6=
A549/V16 MkPTRZBweHSxc3nzJGF{e2G7 M4n4UlUx|ryP NYTINFJkOWh? M4TrO5JmeHKnc4Pld{BVTy:WTT3pcoR2[2WmIHHwc5B1d3Orcx?= M4\IVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUS2NFM{Lz5{NUm0OlA{OzxxYU6=
U1 MojRT4lv[XOnIFHzd4F6 M1njWFExOM7:TR?= NIfYcHYzcA>? MnjlbY5pcWKrdIOgZ4F{eGG|ZT2z NEnxVpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4NFk1Oid-MkW5PFA6PDJ:L3G+
ACH-2 NIi2bIhCeG:ydH;zbZMhSXO|YYm= Mn3tNE0zODEQvF2= M3[5VlJp M{jjRZJm\HWlZYOg[JJ2\y2rbnT1Z4VlKGGyb4D0c5NqeyCjbnSgd5Vje2WzdXXueEBJUVZvMTDy[ZBtcWOjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl6MEm0Nkc,OjV7OEC5OFI9N2F-
U1 M3fTVWFxd3C2b4Ppd{BCe3OjeR?= NEHjNHIxNTFyMN88US=> NXjm[XdOOmh? NVHOVJdOemWmdXPld{BlenWpLXnu[JVk\WRiYYDvdJRwe2m|IHHu[EB{fWK|ZYH1[Y51KEiLVj2xJJJmeGyrY3H0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M2nmdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUiwPVQzLz5{NUm4NFk1OjxxYU6=
HUVEC-2c NIixZ3lCeG:ydH;zbZMhSXO|YYm= MlW5OVDPxE1? M{DGWlZp NWjyTVg6\GWlcnXhd4VlKHSqZTDvfE1NTExvaX7keYNm\CCjdYTvdIhi\3l? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB{MUeyPUc,OjZyMkG3Nlk9N2F-
SP2/0 MlzzRZBweHSxc3nzJGF{e2G7 NULwZWN2OTByzszN M1\0dlFp Mn;SSG1UVw>? MojhZoxw[2u|IITo[UBieG:ydH;zbZMhd2ZiU2CyM|Ah[2WubIO= NIfGUVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC3OFc{Oid-Mk[wO|Q4OzJ:L3G+
CEF NHPGXIlMcW6jc3WgRZN{[Xl? MofMNEw{Oyx4NzyxNFDPxE1? M4XsZVE2dWmw MkTN[I94di2{ZXf1cIF1\XNiUGKg[Y57gW2nIHHjeIl3cXS7IITvJFQx97zHIHH0JFExOM7:TR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFyMkOzPUc,OjZzMEKzN|k9N2F-
BEL-7402 NITud3dCeG:ydH;zbZMhSXO|YYm= NE[2SWszOM7:TR?= NFPMbHIycA>? NFX5SJBifHSnboXheIVlKHSqZTDhdI9xfG:2aXOgbY5lfWO2aX;uJI9nKEmLST2xNOKh MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF4NEe5OUc,OjZzNkS3PVU9N2F-
HepG2 NEPnb3NCeG:ydH;zbZMhSXO|YYm= NET5WmszOM7:TR?= NETrVnQycA>? M3rtcYF1fGWwdXH0[YQhfGinIHHwc5B1d3SrYzDpcoR2[3Srb36gc4YhUUmLLUGwxsA> NHX5b|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG2OFc6PSd-Mk[xOlQ4QTV:L3G+
HGL5 MmjTRZBweHSxc3nzJGF{e2G7 M4\NcFUx|ryP Mo\0NlRp MnXpbY5lcWOjdHXzJIEheHKxdHXjeIl3\SCnZn\lZ5Qh[WejaX7zeEBmfG:yb4Pp[IU> MljSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkmwO|UoRjJ4MU[5NFc2RC:jPh?=
GC1a MnrXRZBweHSxc3nzJGF{e2G7 MYC1NO69VQ>? NXj1fpFoOjSq NUj5XGd3cW6maXPheIV{KGFicILveIVkfGm4ZTDl[oZm[3RiYXfhbY5{fCCndH;wc5Nq\GV? MmX5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkmwO|UoRjJ4MU[5NFc2RC:jPh?=
A549 MXrBdI9xfG:|aYOgRZN{[Xl? NVHqOHdHOjBizszN NYnBXoVxOjSq NEHBW5Rz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KEiIQ2CgeJJm[XSvZX70 NHHDXGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe5OFE1QSd-MkW3PVQyPDl:L3G+
HeLa NF\ZVmpCeG:ydH;zbZMhSXO|YYm= MWixNO69VQ>? NH7Bfo81QGh? M1LoWIRm[2yrbnXzJJRp\SC{YYTlJI9nKGGyb4D0c5NqeyCmcnHtZZRq[2GubIm= NF\W[mg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3NlU1PSd-MkW3O|I2PDV:L3G+
SGC-7901 NWHHXnI{SXCxcITvd4l{KEG|c3H5 NHjJdoQzOMLizszN NGP6XGgycA>? MoiwbY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDvfIFtcXCuYYTpci=> MnLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkewO|YoRjJ3N{[3NFc3RC:jPh?=
MCF-7 M1XZe2Fxd3C2b4Ppd{BCe3OjeR?= M4PIR|Ex|ryP M1znZVJp MV;pcohq[mm2czD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IEPC MnPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MkKxNVQoRjJ3N{KyNVE1RC:jPh?=
A549 NEW1fXBCeG:ydH;zbZMhSXO|YYm= NGLGXXAyOCEQvF2= NWLVSnM4OjSq MlnNSG1UVw>? M3rQUJBzd22xdHXzJGhDSy22cnXheIVlKEF3NEmgZ4VtdCC|dYL2bZZidCBoIHH0eIVvfWG2ZYOgeIhmKGOuZXH2[YQhWEGUUDDlfJBz\XO|aX;u NEjXTmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4N|U3QCd-MkW2PFM2Pjh:L3G+
U87 M3HJ[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW[1NO69VQ>? NWqxNnBwOWh? MmHOdoVkd3[ncoOgZ4VtdCCpcn;3eIgh\nKxbTDUUXohfHKnYYTt[Y51 NEj5e5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4NVY3QCd-MkW2PFE3Pjh:L3G+
Neocortical Neuron Mm\KUoV2em:2b4jpZ4l1gSCDc4PhfS=> MlPPNVAx|ryP NUD4N2hWOWh? M2nHVIFvfGGpb37pfoV{KEixaXHtbYRmKEFvSX7keYNm\CCQZYXyc5RwgGmlaYT5 M1Tke|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nke1NFAyLz5{NU[3OVAxOTxxYU6=
Primary human placental cytotrophoblasts NULNfpE5SXCxcITvd4l{KEG|c3H5 NVj0dG1POzEQvF2= NF21Z4EzPGh? NI\ZUGdFVVOR Ml;sdoV3\XK|ZYOgeIhmKGmwaHnibZRqd25ib3[gNVHPui2KU1SyJIJ6KHS{aXPsc5NidsLi MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ2MkW5Nkc,OjV4NEK1PVI9N2F-
MM M1;1OmFxd3C2b4Ppd{BCe3OjeR?= M3XTTlUx|ryP NUD5dnRnOjCvaX6= Mk[wdIFzfGy7IHnubIljcXS|IGPIT{1qdmS3Y3XkJEBk\WyuIHTlZZRp M3TnUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUOwNFk5Lz5{NUWzNFA6QDxxYU6=
DLD1 MV;BdI9xfG:|aYOgRZN{[Xl? NXS1XmpxOjEEoN88US=> MVOxbC=> NGOxPW5x[XK2bImgdoV3\XK|ZYOgZ4VtdCCjcH;weI9{cXNiY3H1d4VlKGK7IGfTVFE> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV{NEK0Okc,OjV3MkSyOFY9N2F-
MDA-MB-231 MXPDfZRwfG:6aXPpeJkhSXO|YYm= NXXlRXJUOTEQvF2= MXyxbC=> MoL4ZZVodWWwdIOgZ4VtdCCmZXH0bEBi\nSncjDDTHVCKHS{ZXH0cYVvfA>? NEfmNJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWyNVUxOSd-MkW1NlE2ODF:L3G+
MKN28 M3nO[GFxd3C2b4Ppd{BCe3OjeR?= MonSNVDPxE1? MX6zNI1qdg>? MXTEUXNQ MVjpcohq[mm2czDUUmYu|rFicHz1d{BEUFhvaX7keYNm\CCjcH;weI9{cXN? M37yNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUGzPVYxLz5{NUWxN|k3ODxxYU6=
HL-60 NHHCZYJEgXSxdH;4bYNqfHliQYPzZZk> MojzNVAx|ryP M4nFdFFp M2DoXWROW09? MUFCpIlueHKxdnXzJJZq[WKrbHn0fUBw\iCWQ17Bd{11emWjdHXkJINmdGy| NWLG[3dvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NFI6OzJpPkK1OVAzQTN{PD;hQi=>
AGS MXzBdI9xfG:|aYOgRZN{[Xl? MYKxNO69VQ>? M1m4OlFp M4W2VZBz\X[nboTzJIN2emO3bXnuMYlv\HWlZXSgZ4xm[X[jZ3Wgc4Yh[2G|cHHz[U0{NCBvODygZY5lKC17IIDyc5RmcW6| M2DOR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEmyNlE1Lz5{NUS5NlIyPDxxYU6=
A549  NVvyclNSSXCxcITvd4l{KEG|c3H5 NGrqOZMyOM7:TR?= MVqyOIg> M4OxPIJtd2OtczDEUWFUNWmwZIXj[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHHu[EBieG:ydH;0bYMh[2WubDDk[YF1cA>? NXzIV2Y2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QDlpPkK1OFM1QTh7PD;hQi=>
INS-1 MYPBdI9xfG:|aYOgRZN{[Xl? NVnDSI54PTEQvF2= NE\leG03cA>? MU\EUXNQ NFv2eVJl\WO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh\WG{bImgZZBweHSxdHnjJINmdGy|wrC= M{HTclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEOwPFk4Lz5{NUSzNFg6PzxxYU6=
Primary hepatocytes  NXHsVW1RSXCxcITvd4l{KEG|c3H5 MVe1NO69VQ>? Mo\yNVhp NFXCZVJqdmirYnn0d{BieG:ydH;zbZMhd2ZiaHXwZZRw[3m2ZYOgbY5lfWOnZDDifUBC[3RiRDDhcoQhXE6ILd8x NE\5SY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwO|U{QCd-MkW0NFc2Ozh:L3G+
L929-A  MnLqR5l1d3SxeHnjbZR6KEG|c3H5 NU\mVlJOOjEEoN88US=> M2G3S|I1cA>? MlzBbY5pcWKrdIOgWG5H|rFvaX7keYNm\CClZXzsJIRm[XSq M4nRVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{m4OVQxLz5{NUO5PFU1ODxxYU6=
L929-N M1O5VmN6fG:2b4jpZ4l1gSCDc4PhfS=> MlOxNlDDqM7:TR?= NVzNU44yOjSq MWHlcohidmOnczDk[YF1cCC4aXGgZZV1d2O{aX7lJHRPTs7zIIDyc4R2[3Srb36= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7OEW0NEc,OjV|OUi1OFA9N2F-
Cytotoxicity Assay NV\ueXFVS2WubDDWbYFjcWyrdImgRZN{[Xl? MU[yNO69VQ>? NVvySmFPPDiq MnjEdJJmfmWwdDDNTG1FNWmwZIXj[YQh[2WubDDk[YF1cA>? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN7MkGxOkc,OjV|OUKxNVY9N2F-
COS7 NXj2e25JU2mwYYPlJGF{e2G7 M4fzOVEx|ryP MWiyOIg> NEfvd5lqdmO{ZXHz[ZMh[2G|cHHz[UA{NzdiYXP0bZZqfGmncx?= M3nFTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{i0NFI2Lz5{NUO4OFAzPTxxYU6=
COS7 NHTVZmxCeG:ydH;zbZMhSXO|YYm= NV\peXdHOTEQvF2= MVe0PIg> M1;uPZBienSrYXzsfUBxemW4ZX70[YQhTkNzMEGtbY5lfWOnZDDj[YxtKGSnYYTo M3X2XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{i0NFI2Lz5{NUO4OFAzPTxxYU6=
A375 MmjERZBweHSxc3nzJGF{e2G7 NILvPZU{OM7:TR?= NVrLOm5jOmh? Mo[1dJJmfmWwdIOgeIhmKGS{dXetbY5lfWOnZDDQRXJRKGOuZXH2ZYdm MlW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|N{[xNVUoRjJ3M{e2NVE2RC:jPh?=
A549 MX7BdI9xfG:|aYOgRZN{[Xl? NEHmS5U2OM7:TR?= NFfYTIEzPGh? M3fvSpJmfmW{c3XzJJJq[m:|b33lJIJqd2enbnXzbZMh[W6mIHHwc5B1d3OrczDjZZV{\WRiYomgJGNpcWqxbnfkZY4> MorJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|NEm3PFEoRjJ3M{S5O|gyRC:jPh?=
A549 NWjMZ2wxSXCxcITvd4l{KEG|c3H5 NGrtNGgzNjVvMkZOwG0> NUDGb4RkOWh? NYfxOIdK\GWlcnXhd4V{KHSqZTDwc5B2dGG2aX;uJI9nKGGyb4D0c5Rq[yClZXzsd{Bl\XCnbnSgc44h[2:wY3XueJJifGmxboO= NIWyZVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO0NlQzPyd-MkWzOFI1Ojd:L3G+
 NBL-W-S  NHTrRnNCeG:ydH;zbZMhSXO|YYm= MkfmOVDPxE1? MXSxbC=> MYPmeYxtgSC{ZYPjeYV{KGOnbHygeoli[mmuaYT5JIFnfGW{IFfBUnQuPjFidILlZZRu\W62 NWDHbopVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNlMzOjJpPkK1N|I{OjJ{PD;hQi=>
Caki-1  NWToR3pxSXCxcITvd4l{KEG|c3H5 NEjlV3I1OM7:TR?= NWLleJBwPDiq MVPy[YR2[2W|IITo[UBvfW2kZYKgc4YhSW6wZYjpckBXNXCxc3n0bZZmKGOnbHzz MmH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{mxPVEoRjJ3Mke5NVkyRC:jPh?=
769-P NVrDSld1SXCxcITvd4l{KEG|c3H5 NWrVXIZUPDEQvF2= MoXrOFhp MljMdoVlfWOnczD0bIUhdnWvYnXyJI9nKEGwbnX4bY4hXi2yb4PpeIl3\SClZXzsdy=> NIrhdmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PVE6OSd-MkWyO|kyQTF:L3G+
H9c2 NFfMSGNCeG:ydH;zbZMhSXO|YYm= NG\URnQ2OM7:TR?= NFX2fnAycA>? NITiWlJqdmirYnn0d{BFV1hvaX7keYNm\CClYYPwZZNmKDNiYXP0bZZifGmxbjDieZQhdm:2IITo[UBtd3O|IH;mJINmdGy| M4XtdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkizPFE6Lz5{NUK4N|gyQTxxYU6=
K562 NUfnTHZJSXCxcITvd4l{KEG|c3H5 MXy1NO69VQ>? MVe0bC=> NEHZRXRqdmirYnn0d{BL[WNvQT3pcoR2[2WmIHPlcIwh[XCxcITvd4l{ Mk\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{NEG2NVkoRjJ3MkSxOlE6RC:jPh?=
L929 MoLCRZBweHSxc3nzJGF{e2G7 MUCxNO69VQ>? NFm1bpc3cA>? NVvZV4I{cW6mdXPld{Bv\WO{b4D0c5NqeyC5aYToJHRPTg>? NFvEV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5OVY3OCd-MkWxPVU3PjB:L3G+
A549 NInmWmdCeG:ydH;zbZMhSXO|YYm= M{nMU|Ex|ryP MnS2N4g> M1rRVpJm\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3H1d4VlKGK7IGDRVS=> NFjMOYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2NVY6QSd-MkWxOlE3QTl:L3G+
MCF-7 MULBdI9xfG:|aYOgRZN{[Xl? NHzRXXUzOM7:TR?= NHPYSIkzcA>? NYfGO3Mz\GWlcnXhd4V{KEuGUj3zbXJPSS2rbnT1Z4VlKGGyb4D0c5Nqew>? M3Ln[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUiyNlQxLz5{NUG4NlI1ODxxYU6=
OS MkDIRZBweHSxc3nzJGF{e2G7 MmrmNlAwPDEQvF2= MoLVO|Jp MVfpcohq[mm2czDPV{Bk\WyuII\pZYJqdGm2eTDy[YR2[3Srb36gZpkhSzZiY3XyZY1q\GV? Mlz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNUKzPVkoRjJ3MUWyN|k6RC:jPh?=
HUVECs M1LkSWFxd3C2b4Ppd{BCe3OjeR?= NUTJVZFSOTEQvF2= NXr1eIJ{Omh? M1HjVuKhcW6qaXLpeJMh[XCxcITvd4l{KGGwZDDmZYNqdGm2YYTld{BifXSxcHjh[5khcW5iRFXOWlIucW6oZXP0[YQhUFWYRVPz MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF|OEewN{c,OjVzM{i3NFM9N2F-
EA.hy926 M2HGV2Fxd3C2b4Ppd{BCe3OjeR?= NVHP[WJNOTEQvF2= M{DkWlJp NHHpb2XDqGmwaHnibZR{KGGyb4D0c5NqeyCjbnSg[oFkcWyrdHH0[ZMh[XW2b4DoZYd6KGmwIFTFUnYzNWmwZnXjeIVlKEWDLnj5PVI3 M2nWcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUO4O|A{Lz5{NUGzPFcxOzxxYU6=
Ebs MVLBdI9xfG:|aYOgRZN{[Xl? M2TG[VExNTFyMN88US=> NFXhWmgzPGh? MlnGxsBOVVSVIHflcoVz[XSrb36gdoF1\SCmZXPy[YF{\WRiYYOgeIhmKGOxbnPlcpRz[XSrb36gc4Yhgi2YQVSu[o1sKGmwY4LlZZNm\A>? M4G3eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUO0PFE4Lz5{NUGzOFgyPzxxYU6=
Jurkat MXTBdI9xfG:|aYOgRZN{[Xl? NXzaTlZ7OjEQvF2= M33GcVI1cA>? M3T6cWROW09? MVjwZZJ1cWGubImgbY5pcWKrdDDj[YxtKGSnYYToJI9nKEq3cnvheEBk\WyuczDpcoR2[2WmIHL5JFExODV6LV[0JINwdWKrbnXkJJdqfGhiVmDBxsA> M3P5OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUKwO|I{Lz5{NUGyNFczOzxxYU6=
K562 MoLFRZBweHSxc3nzJGF{e2G7 NFfjTW0xNjFvMd88US=> NIK0fI0ycA>? MXrpcohq[mm2czDjcIVifmGpZTDv[kBJW1B7MDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M3;xR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUG5NVg5Lz5{NUGxPVE5QDxxYU6=
IMR-32 NVHRNpl1SXCxcITvd4l{KEG|c3H5 NFTVeWg1OMLizszNxsA> M17XbVJp NI\QdWll\WO{ZXHz[UBqdiCjcH;weI91cWNiY3XscJMh[2:vcHHy[YQhfG9iVD2yJJRwgGmw NGjGV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC4OFc2PSd-MkWwPFQ4PTV:L3G+
RPMI 8226 MYLBdI9xfG:|aYOgRZN{[Xl? NVLTPGh{OTByzszN MkP1NYg> M4jVZYFtdW:|dDDjc41xdGW2ZXz5JIJtd2OtczDj[YxtKGSnYYToJINifXOnZDDifUBv\WO{b4P0ZZRqdi1z M37ZSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEO3PFUyLz5{NUCzO|g2OTxxYU6=
U266  NIrMUnNCeG:ydH;zbZMhSXO|YYm= MmjiNVAx|ryP M1T6NlFp MUnwZZJ1cWGubImgZoxw[2u|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JI5m[3Kxc4TheIlvNTF? M2jpT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEO3PFUyLz5{NUCzO|g2OTxxYU6=
H929  M3n5cGFxd3C2b4Ppd{BCe3OjeR?= MVqxNFDPxE1? NXe4Vmw3OWh? MnXHdIFzfGmjbHz5JIJtd2OtczDj[YxtKGSnYYToJINifXOnZDDifUBv\WO{b4P0ZZRqdi1z MkPsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyM{e4OVEoRjJ3MEO3PFUyRC:jPh?=
MM.1S  NEfiUJVCeG:ydH;zbZMhSXO|YYm= M3rTRVExOM7:TR?= MnTQNYg> M3qzdoVn\mmlaXXueIx6KHC{ZY\lcpR{KHOxcnHm[Y5q[i2rbnT1Z4VlKGOnbHyg[IVifGhiY3;tZolv\SC5aYToJI5m[3Kxc4TheIlvNTF? NGD3cJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCzO|g2OSd-MkWwN|c5PTF:L3G+
C6 NYnmSmpPSXCxcITvd4l{KEG|c3H5 MXm1NO69VQ>? M1\xNFQ5cA>? NH3lSYFxemW4ZX70d{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBk[XW|ZXSgZpkheHKnZ37lco9td26n NHfEXVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxN|Q4QSd-MkWwNVM1Pzl:L3G+
AGS MYjBdI9xfG:|aYOgRZN{[Xl? NX\rWW56PTEQvF2= MVeyOIg> MXThZo9tcXOqczFOtk1t[XCjY3jvcoUucW6mdXPl[EBk\WyuIHTlZZRpKGGwZDDpcohq[mm2ZXSg[5Jwf3Sq M1yxZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEC5Olk5Lz5{NUCwPVY6QDxxYU6=
HeLa  NEjMNo9CeG:ydH;zbZMhSXO|YYm= M3;nVFQx|ryP MlO4NlRp MkDkbY5pcWKrdIOgeIhmKGmwY4LlZZNm\CCjcH;weI9{cXNiaX7keYNm\CCkeTDzbXJPTjF{MR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl{OE[4OUc,OjR7Mki2PFU9N2F-
THP-1  MVXBdI9xfG:|aYOgRZN{[Xl? M3XJVFEx|ryP M2jwOlFp NEK3cYZz\WS3Y3XzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEGOQT3TSHTDqA>? NWXYdWxGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NlM3PTNpPkK0PVI{PjV|PD;hQi=>
P815  M{LIPGFxd3C2b4Ppd{BCe3OjeR?= M1T2flExOCEEtV2= NWWz[5l5OTKq NUHKWZFuTE2VTx?= MoHtbY5pcWKrdIOgeolzfXNvaX7keYNm\CCjcH;weI9{cXN? NH;6dmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmyN|I4Oyd-MkS5NlMzPzN:L3G+
HCT116 NHuwNFFCeG:ydH;zbZMhSXO|YYm= NEe5VowzOM7:TR?= M3\sdlI1cA>? MX7hZpJw\2G2ZYOgc4YhXFFvaX7keYNm\CCjcH;weI9{cQ>? M1n6[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEmwOFQ6Lz5{NEi5NFQ1QTxxYU6=
SW1116  MnPBRZBweHSxc3nzJGF{e2G7 M3yzV|ExKML3TR?= MlfsNYg> MmHESG1UVw>? NFrlTpJz\XO2cnHpcpMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBo\W[rdHnubYIheGy3czDk[YNqfGGkaX7l M4[z[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
LOVO M4\uWmFxd3C2b4Ppd{BCe3OjeR?= NWjRVVBxOTBiwsXN MWqxbC=> NX7TfYxtTE2VTx?= Mon5doV{fHKjaX7zJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomg[4VncXSrbnniJJBtfXNiZHXjbZRi[mmwZR?= NFXSe|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O|QzQDZ:L3G+
SNU449  NIHpZ2tCeG:ydH;zbZMhSXO|YYm= Mn\BNlDPxE1? MkT5OFhp MV\EUXNQ NIe3Rnpl\WO{ZXHz[ZMhdWmULUS1NU1qdmS3Y3XkJIFxd3C2b4TpZy=> NIDUXYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0NVY{QCd-MkS4OFE3Ozh:L3G+
Caco-2 Ml34RZBweHSxc3nzJGF{e2G7 NHm0bHo1OOLCid88US=> MofiOIg> MXfwdoV3\W62czDTWE04NWmwZIXj[YQhYk9vMTDjbIFv\2W|IHHu[EBVTVJiZILvdC=> NVzPOIN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NlIyQDNpPkK0PFIzOTh|PD;hQi=>
macrophage M1TWd2NmdGxiVnnhZoltcXS7IFHzd4F6 Mli1NE0zODEQvF2= NX7CcGo{OjSq NYn4dldLcW6mdXPld{BVVkZvIHHu[EBTcXB|LXTldIVv\GWwdDDu[YNzd3C2b4Ppd{BqdiCvYXPyc5Bp[Wen MkDhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5OUm1OlUoRjJ2N{m5OVY2RC:jPh?=
AGS  MVHBdI9xfG:|aYOgRZN{[Xl? M{DRW|Ix|ryP NWTiWFlkOTKq MXrEUXNQ Mnj5doVlfWOnczD0bIUhcW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBz\XOyb37z[UB1dyC2aHWgSZRQSWNiZoLhZ5Rqd25iaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NU\rcY9VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PFk4ODNpPkK0O|g6PzB|PD;hQi=>
LCC9  NITIWVRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NEe2OlkyODEQvF2= Mn3uOYQ> NX\MZllt[myxY3vzJINmdGxiZHXheIghcW6mdXPl[EBjgSClb33ibY5ifGmxbjDMNVch[W6mIHPocI9zd3G3aX7l NHrLVJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe4OVI2Pid-MkS3PFUzPTZ:L3G+
U937  M{PlTY5m[3KxcITvd4l{KEG|c3H5 NHvVWXAyOMLizszN MnPyN|DDqG2rbh?= MmqxbY5lfWOnczDu[YNzd3C2b4Ppd{Bkd22kaX7lJJdqfGhiVF7G NW[3bHMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3O|M4PTZpPkK0O|c{PzV4PD;hQi=>
T cell NEfhNG1E\WyuIIDyc4xq\mW{YYTpc44h[XO|YYm= NEfpdFUxNTFyMN88US=> MW[3Nog> MkDUTWM2OD15MDFOwG0tKGmwaHnibZR{KGGwdHmtR2Q{NWmwZIXj[YQhXCClZXzsJJBzd2yrZnXyZZRqd25iaX6gVGJOS3N? NXHQZVhLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3Olg4ODdpPkK0O|Y5PzB5PD;hQi=>
HeLa  NGDleIFCeG:ydH;zbZMhSXO|YYm= NH23dWk2OM7:TR?= NIDlZXE4Omh? MljOSG1UVw>? MkLkdJJmfmWwdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkh[2:vcH;1coR{yqBz4pETOS=> Mn24QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5NUS3PFYoRjJ2N{W0O|g3RC:jPh?=
HeLa  MV3BdI9xfG:|aYOgRZN{[Xl? MWKxNFDPxE1? Mn\xNlRp M17sb5N2eHC{ZYPz[ZMhemG2ZTDv[kBk\WyuIHTlZZRpKGmwZIXj[YQh[nlib4\ldoV5eHKnc4Ppc44hd2ZiR{W5V{BweiCJN{HSJJAyPTCpbIXl[OKh MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd{MkS2PEc,OjR5MkK0Olg9N2F-
IEC-6  M4rxVmFxd3C2b4Ppd{BCe3OjeR?= NGDqZVIyOM7:TR?= Mo\RNlRp MnfQdJJmfmWwdIOgWINlSS2rbnT1Z4VlKGOjc4Dhd4UuOyClbHXheoFo\SCjbnSg{tIu[2G2ZX7pckBl\We{YXTheIlwdg>? NX;tSHhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVE2PzFpPkK0O|EyPTdzPD;hQi=>
HCT116 M{HifmFxd3C2b4Ppd{BCe3OjeR?= M2S4[VIx|ryP NEj2VZIzPGh? NYr0bo83cW6qaXLpeJMhfGinIHPlcIwh[XCxcITveIlkKGmwZIXj[YQh[nliWVzUNlA2 MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDdzM{ixNkc,OjR5MUO4NVI9N2F-
L02 M3fXfGFxd3C2b4Ppd{BCe3OjeR?= NXTRWpg2OjEQvF2= M{SyWVFp MnjudJJmfmWwdIOgeIhmKGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhVUWKUB?= Moe0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5ME[0OlEoRjJ2N{C2OFYyRC:jPh?=
A375 NW\0NG86SXCxcITvd4l{KEG|c3H5 MofGNlDPxE1? NXHhR2liOjSq NVPmOmZoemW4ZYLz[ZMh\GmlaYTybY5wdmViQj3pcoR2[2WmIHHwc5B1d3Orcx?= M4fsVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nkm5NVEyLz5{NE[5PVEyOTxxYU6=
HDPC MX7BdI9xfG:|aYOgRZN{[Xl? MVK1NOK2VQ>? NGPSWVgzPGh? NHjtfG9qdmirYnn0d{BPVy2rbnT1Z4VlKGGyb4D0c5Nqew>? Mn;4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{S1PVMoRjJ2NkO0OVk{RC:jPh?=
A549 MV3BdI9xfG:|aYOgRZN{[Xl? Mly3OVDDvU1? M{TIOVJp NUPIUGdvTE2VTx?= MXzwdoV3\W62czD0bIUhcHmyb3TpdIxwcWRiRF7BJINwdnSnboSgdIhie2ViaX7keYNm\CCkeTDj[ZBp[WyxY3jyc41qdg>? NWHxWVFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1PFgyOzVpPkK0OVg5OTN3PD;hQi=>
 BV-2 MnPCRZBweHSxc3nzJGF{e2G7 NHTpVnkzOM7:TR?= MoeyNog> M3rrWpN2eHC{ZYPz[ZMhXVZvaX7keYNm\CClaILvcYF1cW5iZYj0dpV{cW:wIHHu[EBlcWyjdHnvckBw\iC2aHWgcpVkdGWjcjDlcpZmdG:yZR?= MkPCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NUixNVgoRjJ2NUW4NVE5RC:jPh?=
HT-29  NEDKfVVCeG:ydH;zbZMhSXO|YYm= NUnBcJA3PTEQvF2= MVK0PIg> MnznZoxw[2u|IITo[UBieG:ydH;zbZMhcW6mdXPl[EBjgSCtYXXtdIZmem:u NGWyRWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW0PVE4PSd-MkS1OFkyPzV:L3G+
A549 MYjBdI9xfG:|aYOgRZN{[Xl? NV7QeG0zPc7:TR?= MlXqNlRp M4LDXpN2eHC{ZYPz[ZMhPmYEoDjCT|ExODRyKT3pcoR2[2WmIHHwc5B1d3Orc9Mg NVHYeWw1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1Nlk5PzFpPkK0OVI6QDdzPD;hQi=>
MIA-PaCa-2 NFvrWXpCeG:ydH;zbZMhSXO|YYm= NIHzSHYzPGh? MV3icI9kc3NiY3zlZZZi\2Vib3[gZ4F{eGG|ZT2zJIlv\HWlZXSgZpkh[m:2aDDBMVQ1OzZ3NDDhcoQheGGlbHn0ZZhmdA>? M4PRcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUGwPVkzLz5{NEWxNFk6OjxxYU6=
Caki-1 MkXiRZBweHSxc3nzJGF{e2G7 Ml\PNlDPxE1? MXyxbC=> NYHvcFV2cW6qaXLpeJMhW0OSLXnu[JVk\WRiYYDvdJRwe2m| NGHWRXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwOFY5OSd-MkS1NFQ3QDF:L3G+
CLL Mn7ERZBweHSxc3nzJGF{e2G7 NFHm[YIzPc7:TR?= NEHadnUycA>? NUm2UG1beGG{dHnhcIx6KGKub3Prd{BOVE5{MkO4MYlv\HWlZXSgZ4VtdCCjcH;weI9{cXN? NEDIb|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2O|Y{PCd-MkS0Olc3OzR:L3G+
SGC-7901 MoPvRZBweHSxc3nzJGF{e2G7 MlzMNVDPxE1? M{XLclI1cA>? NU[2UHBueHKxbX;0[ZMhfGinIFPHTWkucW6qaXLpeIVlKGOnbHyg[5Jwf3SqIHnuJHNISy15OUCxJINmdGy| NX\oVlRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVQ1QDhpPkK0OFU1PDh6PD;hQi=>
k1735 MVvBdI9xfG:|aYOgRZN{[Xl? NYeyN4hVOjEkgJpOwI0> M3;KTVRp M4TpSIlvcGmkaYTzxsBU[Wyvb37lcIxiNWmwZIXj[YQh[XCxcITvd4l{ NH25TZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1NVEyPid-MkS0OVEyOTZ:L3G+
HaCaT  M{nJXWFxd3C2b4Ppd{BCe3OjeR?= NEH5N44yODEQvF2= NVX5epczOWh? MWficJVvfHNiVW\CMYlv\HWlZXSgZZBweHSxc3nzJIlvKEijQ3HUJINmdGy| MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3Nkm5O{c,OjR|NU[5PVc9N2F-
podocytes MYHBdI9xfG:|aYOgRZN{[Xl? NHi1W40zODEQvF2= NUSyWIFDPmh? MXjpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUA{NDRvRFfF NFjmW5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOzO|c4Pyd-MkSzN|c4Pzd:L3G+
 KB M17UTWFxd3C2b4Ppd{BCe3OjeR?= MmDZOVDPxE1? NUXLPXBwOjSq M3\YWolvcGmkaYTzJGxq[29vQT3pcoR2[2WmIHPhd5Bie2VvMzDhcoQhWEGUUDDhZ5RqfmG2aX;u NFL3VHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOzO|Q6Oid-MkSzN|c1QTJ:L3G+
 HepG2  MVfBdI9xfG:|aYOgRZN{[Xl? MmXsNVDPxE1? MlHGNlRp NEfK[lhz\WS3Y3XzJJZqdWWwdHnuJINt\WG4YXflJINifXOnZDDifUBNWFN? NXv5PXVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlU5OTZpPkK0N|I2QDF4PD;hQi=>
fetal rat lung fibroblasts  MWnLbY5ie2ViQYPzZZk> M4Tzflgx|ryPwrC= Ml3RNYg> MXTpcohq[mm2czDDZZNx[XOnLUOgZYN1cX[rdIpCpC=> NIi0XGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOxOFE{PSd-MkSzNVQyOzV:L3G+
PDL fibroblasts NVP1W2ZMU2mwYYPlJGF{e2G7 NUDM[phMQDEQvF5CpC=> MlyxNYg> NUn6eIY{cW6qaXLpeJMhS2G|cHHz[U0{KGGldHn2bZR6yqB? Mn3uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUSxN|UoRjJ2M{G0NVM2RC:jPh?=
MB49 MnzVRZBweHSxc3nzJGF{e2G7 M2\q[FIx|ryP NEj5UXQycA>? NE\neZBz\X[ncoPld{BEVS2rbnT1Z4VlKGOnbHyg[IVifGh? Mm\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OEK0N|MoRjJ2MkiyOFM{RC:jPh?=
5637 MmX4RZBweHSxc3nzJGF{e2G7 NVP2W|F3OjEQvF2= NVfEWlJGOWh? M4LWZZJmfmW{c3XzJGNONWmwZIXj[YQh[2WubDDk[YF1cA>? NUfUfGd4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFI1OzNpPkK0NlgzPDN|PD;hQi=>
A549  M4D0[GFxd3C2b4Ppd{BCe3OjeR?= NIixUYMyODEQvF2= MV[0PIg> MUfzeZBxemW|c3XzJJRp\SCjcH;weI9{cXNiY3H1d4VlKGK7IIDpdIVzcW6nwrC= NVL0UZpWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|IzODFpPkK0NlczOjBzPD;hQi=>
MCC-2 NFO4VoFCeG:ydH;zbZMhSXO|YYm= NGOzW2QzOCEQvF2= M3HPclZp MWLEUXNQ NELlb5JjdG:la4OgbY5lfWOnZDDifUB{fGG3cn;zdI9zcW6nIHHwc5B1d3OrczDj[YxtKGSnYYTo Mmn4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NkK2OVgoRjJ2Mk[yOlU5RC:jPh?=
ADF NHSxbo9CeG:ydH;zbZMhSXO|YYm= M1iy[|Qx|ryP M1PvOFZp NVjWcplYcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliY3jlcY91cGW{YYD5JJBtfXNiWn7DcFI> NWrjeVZyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlgzOzJpPkK0NlI5OjN{PD;hQi=>
 U373  MV7BdI9xfG:|aYOgRZN{[Xl? MUW0NO69VQ>? MX[2bC=> MXPpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBkcGWvb4To[ZJieHlicHz1d{BbdkOuMh?= Mn3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
RKO-HIPK2i NHrGbZVCeG:ydH;zbZMhSXO|YYm= MWi0NO69VQ>? NEHUR4I3cA>? NIjleWVqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClaHXtc5Rp\XKjcImgdIx2eyCcblPsNi=> M2fuO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkK4NlMzLz5{NEKyPFI{OjxxYU6=
HEC-1B MojnRZBweHSxc3nzJGF{e2G7 M{nkeFIx|ryP MWOxbC=> MYny[YR2[2W|IGTQMYlv\HWlZXSgZZBweHSxc3nzxsBk[XOyYYPlMVMh[W6mIHPhd5Bie2VvOR?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzM{O1PEc,OjR{MUOzOVg9N2F-
4T1 NILlN5RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NFmyOGQzNjVvMUFOwG0> NGHhdWw1cA>? MXzEUXNQ NEHsU4Rz\XOldXXzJJRp\SCleYTveI95cWOrdImgc4YhPFRzIHPlcIx{KGmwZIXj[YQh[nliU2DEWEBqdiCjIHPvcoNmdnS{YYTpc44h\GWyZX7k[Y51KG2jbn7ldi=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJyNkG2NUc,OjR{ME[xOlE9N2F-
OS  MULBdI9xfG:|aYOgRZN{[Xl? MmHHNlDPxE1? NVjUZnRWPGh? NWDIZ3lmcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigZ4F2e2WmIHL5JHBFXA>? NU\0dYxZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFQ6OzdpPkK0NlA1QTN5PD;hQi=>
RPE NUnrO4ZUSXCxcITvd4l{KEG|c3H5 NGfJTpEyODEQvF2= NUTBVmY1PDiq MlfCdIFzfGmjbHz5JIlvcGmkaYTzJJRp\SClYXzwZYlvNTFiYX7kJE0zKGGldHn2ZZRqd25iYYOge4VtdCCjczD0bIUh[2G|cHHz[UBi[3SrdnH0bY9v NXLlW5FiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFIxPTJpPkK0NlAzODV{PD;hQi=>
UD29a NVvvWmh[SXCxcITvd4l{KEG|c3H5 Ml62OVDPxE1? Mn3oNlRp MkexbY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiY3H1d4VlKGK7IF7VWFM> M3fTelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUmwOVc1Lz5{NEG5NFU4PDxxYU6=
SH-SY5Y  MVTBdI9xfG:|aYOgRZN{[Xl? M2jiOFEx|ryP NFqyNmEzPGh? NH\FOm1ifHSnboXheIV{KGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KEiQRT2ySGc> MnKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNEW0OlMoRjJ2MUS1OFY{RC:jPh?=
HeLa  NYjld|c{SXCxcITvd4l{KEG|c3H5 MYixNE8zOM7:TR?= NVzEd5dKOjSq Ml3HbY5pcWKrdIOgeIhmKGGldHn2bZR6KG:oIITo[UBu[Wqxcnn0fUBw\iC2aHWgcYVu[mW{czDv[kB1cGViY3HzdIF{\S2oYX3pcJk> MoHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{eyOlYoRjJ2MUO3NlY3RC:jPh?=
SCCVII  MWrBdI9xfG:|aYOgRZN{[Xl? NEDaZm8zPc7:TR?= M1HqZ|Fp NYPocIlwcW6qaXLpeJMhfGinIHPlcIwhc2mubHnu[{Bi\nSncjDkZZNifGmwaXK= Ml\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMk[0OlQoRjJ2MUK2OFY1RC:jPh?=
RAW 264.7 NV7JS3J[SXCxcITvd4l{KEG|c3H5 NVvKNGFjOjEQvF2= MVexPIg> NH7RSHNqdmO{ZXHz[ZMhVEN|LVnJM:6zNWGldHnuJJJifGmxIHPvcZBiemWmIITvJGVEXFZvTV;TJJRz\WG2bXXueEBwdmy7 M3TiUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUG2O|A4Lz5{NEGxOlcxPzxxYU6=
A549  MVfBdI9xfG:|aYOgRZN{[Xl? MXKyMlXPxE1? NIf4SIEzPGh? NEG3eVVl\WO{ZXHz[ZMhd3KrZH;ubY4ucW6mdXPl[EBifXSxcHjh[5kh[XNid3XscEBidmRiTH;zd{Bw\iEQlN-IcUBidHOxIH;jZ5VzemWmIHT1dolv\yCjdYTvdIhi\2mlIIDyc4Nme3N? MnT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEK1NlIoRjJ2MUCyOVIzRC:jPh?=
UM-SCC-10A  M4CzOGFxd3C2b4Ppd{BCe3OjeR?= M1jUV|UxyrWP MUGybC=> MnXodoVlfWOnczDhdI9xfG:|aYOgbY5lfWOnZDDifUBpcWeqLXTvd4UhcXOxYXzhcpRwdGGldH;u[S=> MlW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUi3OVMoRjJ2MEm4O|U{RC:jPh?=
BGC-823 NUnHZXRsSXCxcITvd4l{KEG|c3H5 NH;wd|IyOCEQvF2= MkD1NlRp M3PEWpJm\HWlZYOgeIV1emGwZILpcoUucW6mdXPl[EBieG:ydH;zbZPDqA>? M2qzVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm4OVEyLz5{NEC5PFUyOTxxYU6=
Nalm-6  MXvBdI9xfG:|aYOgRZN{[Xl? NHv6SYozOM7:TR?= NWKxNnl3PGh? MnrDbY5pcWKrdIOgZ4F{eGG|ZT24JIFv\CClYYPwZZNmNTNiYXP0bZZifGmxbjDhcoQhWEGUUD2xJINt\WG4YXfl MknrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyM{m5OlcoRjJ2MEO5PVY4RC:jPh?=
KHOS  NHr6ZYJCeG:ydH;zbZMhSXO|YYm= MkPtOFDPxE1? NV7UfGxiOjSq NVv5dno1[myxY3vzJJRp\SCrbnPy[YF{\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZT2zJIFv\CCSQWLQJIlv\HWlZXSgZpkhSkKPRERCpC=> Mn71QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMkWzOlEoRjJ2MEK1N|YyRC:jPh?=
G292 NEDXOpBCeG:ydH;zbZMhSXO|YYm= NHf2OnU1OM7:TR?= NIXSWmkzPGh? Mo\oZoxw[2u|IITo[UBqdmO{ZXHz[YQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgRmJOTDQEoB?= NGPQXIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyOVM3OSd-MkSwNlU{PjF:L3G+
MG-63 M3;QVmFxd3C2b4Ppd{BCe3OjeR?= MYi0NO69VQ>? MnS3NlRp NXL0XZdr[myxY3vzJJRp\SCrbnPy[YF{\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZT2zJIFv\CCSQWLQJIlv\HWlZXSgZpkhSkKPRERCpC=> M3vQ[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK1N|YyLz5{NECyOVM3OTxxYU6=
EBV-transformed B cells MlzKRZBweHSxc3nzJGF{e2G7 MVqyNO69VQ>? NX7VUlZ1Omh? M{C4NmROW09? M1myb4Jtd2OtczDhcpRqNUOGOECgZY5lKGGwdHmtR2Q5PiCjboTpZo9lgS2rbnT1Z4VlKGGyb4D0c5Nqew>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDByOE[yPEc,OjRyMEi2Nlg9N2F-
IM-9 B M{jCWGFxd3C2b4Ppd{BCe3OjeR?= NHrmb3YzOM7:TR?= NGrXe3UzcA>? NV3XOGNSTE2VTx?= MWjicI9kc3NiYX70bU1ETDhyIHHu[EBidnSrLVPEPFYh[W62aXLv[JkucW6mdXPl[EBieG:ydH;zbZM> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDByOE[yPEc,OjRyMEi2Nlg9N2F-
CNE1 MlqxRZBweHSxc3nzJGF{e2G7 NIi2NI4zOM7:TR?= M{LTZVQ5cA>? NHft[nljdG:la4OgUGsuSS2rbnT1Z4VlKGGyb4D0c5Nqew>? NEezO409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m4OVAzQSd-MkO5PFUxOjl:L3G+
CNE2 MWrBdI9xfG:|aYOgRZN{[Xl? NHTxfYEzOM7:TR?= Mn7tOFhp M1rhbYJtd2OtczDMT{1CNWmwZIXj[YQh[XCxcITvd4l{ M1m4R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUi1NFI6Lz5{M{m4OVAzQTxxYU6=
HL-60 MnfJRZBweHSxc3nzJGF{e2G7 NWDkcm5LPTEEtV2= NG\EZXIycA>? NX;jeG9{TE2VTx?= M3TGSIlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEKDMUS1 MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl2OEe1NUc,OjN7NEi3OVE9N2F-
L929 NXHRe5g3SXCxcITvd4l{KEG|c3H5 NHPneVYyNjJ34pETOeKh|ryPwrC= NUXZUXFoOjSq NX;2OmFx[XWpbXXueJMhXE6IzsGtbY5lfWOnZDDu[YNzd3C2b4Ppd{BidmRiYYX0c5Bp[We7 NHzpbJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0NVc3QSd-MkO5OFE4Pjl:L3G+
Jurkat M4XUVGNmdGxiVnnhZoltcXS7IFHzd4F6 M2\iOFLPxE1? MnLGOIg> MYHy[ZZmenOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh|rJvY3H0[Y5qdiCmZXPy[YF{\SCkeTDIV{1CW0F? MkPOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6OU[wOlEoRjJ|OEm2NFYyRC:jPh?=
RAW264.7  MYjBdI9xfG:|aYOgRZN{[Xl? MWWxNEDPxE1? MVuyOIg> M364cIRm[3KnYYPld{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgS2E> M1;xcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEKwNlA{Lz5{M{iyNFIxOzxxYU6=
A431  NXy3W2dmU2mwYYPlJGF{e2G7 NYT1bIIxPDEQvF2= NVXNOWc{Omh? M2rIcoJtd2OtczDmbZNmfGmwLXnu[JVk\WRiY3zlZZZi\2Vib3[gZ4F{eGG|ZYOgZY5lKFCDUmC= M3TibFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OECwNFU5Lz5{M{iwNFA2QDxxYU6=
Jurkat NILxbZBE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MoDFNlUuOTByzszN NX3vfHZ2Pmh? M1PHdolvcGmkaYTzJJouTkFvQ13LMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MkH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{O1N|IoRjJ|OUOzOVMzRC:jPh?=
COS-7  M2XGPGZ2dmO2aX;uJGF{e2G7 NWjENGNiPTEEtV2= MX2yOIg> MkDJZYZn\WO2czD0bIUheHKxY3Xzd4lv\yCxZjDBWG4yeyC5aYToJJBwdHmTIITyZYN1ew>? NVLXdHllRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5N|MzODhpPkKzPVM{OjB6PD;hQi=>
CAL27 M3HFPGFxd3C2b4Ppd{BCe3OjeR?= NEHmVJkyOCEQvF2= NXXQXnpvOmh? NYPOSHFVe3WycILld5NmeyCFdYKtUnB{NXKnZIXj[YQhfmmjYnnsbZR6KGK7IIXwJJRwKDlyJR?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlzN{O5Okc,OjN7MUezPVY9N2F-
THP-1  M3XCdGFxd3C2b4Ppd{BCe3OjeR?= NGHOeHQyOC13MN88US=> M33rRVJp MVHpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUB1emmydH;sbYRm M{nZUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUCwNlk6Lz5{M{mwNFI6QTxxYU6=
K562 M3vke2Fxd3C2b4Ppd{BCe3OjeR?= MlvhNlDPxE1? NF\mbmw1QGh? M{fScolvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KDRvTWW= Mlv3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{[4NlYoRjJ|OEe2PFI3RC:jPh?=
HL-60 NGX2fI1CeG:ydH;zbZMhSXO|YYm= NHzleYMyODEQvF2= MlniNlRp MnzKbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkh[WKrZYThcoUh\Gm2ZYLw[Y5me8Li NXX3TmhTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OlU4PzhpPkKzPFY2Pzd6PD;hQi=>
HeLa  NYjkTY5LSXCxcITvd4l{KEG|c3H5 Mn[2NlDPxE1? M1H1SlJp MXPpcohq[mm2czDEUW1RKGmwZIXj[YQh[XCxcITvd4l{ M{fQXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OE[zPVY3Lz5{M{i2N|k3PjxxYU6=
U251 M{TxVmFxd3C2b4Ppd{BCe3OjeR?= NUTvRVNWOjEQvF2= NUPGNYtROjSq NFjkclFqdmirYnn0d{Bk[XO2aXPpckBqdmS3Y3XkJGczN01icHjhd4Uh[XK{ZYP0JIFv\CCjcH;weI9{cXN? MlS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MU[4NVYoRjJ|OEG2PFE3RC:jPh?=
HL-60 NIfQfHdCeG:ydH;zbZMhSXO|YYm= NEnDemU2OML3TR?= MnnNOIg> M4nWWIJtd2OtczD0bIUh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|LDCtPUwh[W6mIGDBVnAhcW6mdXPl[EBjgSB4cx?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyNEewOkc,OjN6MES3NFY9N2F-
Ec-109 MlW0RZBweHSxc3nzJGF{e2G7 NGPVV|AyOCEQvF2= M1i0R|Q5cA>? NEDNV49z\XC{ZYPz[ZMhWEVvbXXkbYF1\WRiRXOtNVA6KGOnbHygZZBweHSxc3nz M1TkS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{iyOlQyLz5{M{e4NlY1OTxxYU6=
RKO MoSxR4VtdCCYaXHibYxqfHliQYPzZZk> M3O5TVExKM7:TR?= MWGyOIg> MUPpcohq[mm2czD0bIUh\GWlcnXhd4Uhd2ZiY3XscEB3cWGkaXzpeJkh[2G3c3XkJIJ6KESDQh?= MkjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NUiwOlQoRjJ|N{W4NFY1RC:jPh?=
T98G MoXZR4VtdCCYaXHibYxqfHliQYPzZZk> NEXFSlcyNTFyMN88US=> MnXpNlRp MUjpcZBzd3[nczDj[YxtKH[rYXLpcIl1gSClb4Ty[YF1dWWwdDD3bZRpKFSV MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd4OUK3OUc,OjN5NkmyO|U9N2F-
Jurkat MmToR4VtdCCYaXHibYxqfHliQYPzZZk> MVSxNFAuOjByzszN NInm[3ozPGh? NGrCfFhqdmirYnn0d{BJ[UF2IHnu[JVk\XNiYYDvdJRwe2m|IHTldIVv\GWwdDDv[kBkd26lZX70doF1cW:wwrC= MnXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5M{K0PFEoRjJ|N{OyOFgyRC:jPh?=
Molt-3  M1PHXGFxd3C2b4Ppd{BCe3OjeR?= NFn0Rlc2OM7:TR?= MX[ybC=> NEK2XFZz\WS3Y3XzJI1mdGG2b37pck1qdmS3Y3XkJIFxd3C2b4Ppdy=> M17FZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{K1NFE{Lz5{M{eyOVAyOzxxYU6=
hMSC12 NV3qRWdWSXCxcITvd4l{KEG|c3H5 Ml:3NVAx|ryP NWLJ[m5qPGR? MWjpcohq[mm2czDvd5Rmd2enbnnjJIN2dHS3cnWtbY5lfWOnZDDj[YxtKGSnYYToJIFv\CClYXzjbYZq[2G2aX;u M3HjOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkW3PFIzLz5{M{[1O|gzOjxxYU6=
HM7  MnXDRZBweHSxc3nzJGF{e2G7 MXKyNO69VcLi NVPKU3VJOWh? NWrSZ|A6[myxY3vzJIFxcWO3bHHy[Y4hSSCjY3X0ZZRmNWmwZIXj[YQh[2G|cHHz[U0{KGGldHn2ZZRqd25iYX7kJHBCWlBiY3zlZZZi\2V? M3;VdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUizOFEzLz5{M{W4N|QyOjxxYU6=
Hep-2  NUfUc3U5SXCxcITvd4l{KEG|c3H5 NUjzXpF3OjEQvF5CpC=> MlfPNlRp MkGxSG1UVw>? NHXrfIJidGyndnnheIV{KHSqZTDk[YNz\WG|ZTDv[kBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JJNqdGmkaX7pci=> NITSfGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W4NFA{Oid-MkO1PFAxOzJ:L3G+
HSCs NITteIRCeG:ydH;zbZMhSXO|YYm= M3rHZ|Ux|ryP MXKyOIg> MXrEUXNQ NHzyNFhqdmirYnn0d{BvcWyxdHnubYIucW6mdXPl[EBFVkFiZHHtZYdmKGmwZHnjZZRm\CCkeTDQRXJRKGOuZXH2ZYdm NETNbFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5PVg4PCd-MkO0PVk5PzR:L3G+
HL-60  M1q3W2N6fG:2b4jpZ4l1gSCDc4PhfS=> NFnDWlQzOM7:TdMg MYKyOIg> MkS0doVlfWOnczD0bIUh[3m2b4TvfIlkKGWoZnXjeEBw\iCHcom1 MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7NES4NEc,OjN2OUS0PFA9N2F-
HA M3joNmFxd3C2b4Ppd{BCe3OjeR?= MluxOVDPxE1? NYO2UmdjOjSq NVPrUFFUe3WycILld5NmeyC2aHWgZ4xm[X[jZ3Wgc4YhWEGUUDDhcoQh[2G|cHHz[U0{NCBvNzygZY5lKC17IHnu[JVk\WRiYomgZo9zfGW8b33pZk9USUiD NHLi[po9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S3OVk2Pid-MkO0O|U6PTZ:L3G+
C666-1 M1nZXGFxd3C2b4Ppd{BCe3OjeR?= MlzHOVDPxE1? M{TPdlI1cA>? NXXpO|Rse3WycILld5NmeyC2aHWgZ4xm[X[jZ3Wgc4YhWEGUUDDhcoQh[2G|cHHz[U0{NCBvNzygZY5lKC17IHnu[JVk\WRiYomgZo9zfGW8b33pZk9USUiD M1\TclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEe1PVU3Lz5{M{S3OVk2PjxxYU6=
Hepa1-6 NVfNZXBCSXCxcITvd4l{KEG|c3H5 NXHDb4VtPTEQvF2= NF;qfFQzcA>? NEPpWYtqdmirYnn0d{BkfXKldX3pckBidmRicnXzeoVz[XS{b3ytbY5lfWOnZDDhdI9xfG:|aYO= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2Nke1N{c,OjN2NE[3OVM9N2F-
PLC/PRF/5c M4rsb2Fxd3C2b4Ppd{BCe3OjeR?= MmX3OVDPxE1? MXmxbC=> MmPZdJJmfmWwdIOgZZBweHSxc3nzJJRzcWepZYLl[EBjgSCFSV6= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR|OEiyOEc,OjN2M{i4NlQ9N2F-
HCT116 M2q4XGFxd3C2b4Ppd{BCe3OjeR?= M2nzRVExOM7:TR?= M3fSfVEvPWh? NGTMWXZqdmirYnn0d{BQey2rbnT1Z4VlKGOnbHyg[IVifGh? M{\vbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{S0N|U{Lz5{M{e0OFM2OzxxYU6=
HCEC MYrGeY5kfGmxbjDBd5NigQ>? MXi1NO69VQ>? NFHpeFE4Omh? MmrpSG1UVw>? M17EPZJme3SxcnXzJI9nKHSqZTDuc5Ju[WxiSFPFR{BxcGWwb4T5dIU> MlfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NEKwNVEoRjJ|N{SyNFEyRC:jPh?=
Primary rat cerebral cortical neurons NHzaTYZCeG:ydH;zbZMhSXO|YYm= NF7ve20yODEQvF2= MWixbC=> M1HuXJBz\X[nboTzJGNlNWmwZIXj[YQh[XCxcITvd4l{KGGwZDDj[YxtKGSnYYTo MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd2MUOxO{c,OjN5NEGzNVc9N2F-
MDA-MB-231  NILWXGRCeG:ydH;zbZMhSXO|YYm= MmPFNlXPxE1? MmrsNlRp MWjhZpJw\2G2ZYOgZ5l1d3SxeHnjbZR6KGGwZDDjcIVifmGpZTDv[kBk[XOyYYPlMVMh[W6mIGDBVnAhcW6mdXPl[EBjgSCUZ{O= NWXM[WIyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzN|c2PDRpPkK1N|M4PTR2PD;hQi=>
NLRP3-Tet-on-MC/9  NULQNnZPSXCxcITvd4l{KEG|c3H5 M{PadlExNTRyzszN MnKxNVJp MnLyZZR1\W63YYTld{B1cGViY3XscEBl\WG2aDDjZZV{\WRiYomgR2FRWy2jc4PvZ4lifGWmIF7MVnA{KG23dHHueJMhcW5idHjlJHRmfC2xbjDzfZN1\W1? M4rjdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{CzN|g6Lz5{M{ewN|M5QTxxYU6=
KNS42  MWfBdI9xfG:|aYOgRZN{[Xl? MmfBOVDPxE1? MXWxbC=> NX\Yb2RiemWmdXPld{Bk\WyuIHTlZZRpKGGwZDDjc41xdGW2ZXz5JIFjd2yrc3jl[EBk[XOyYYPlJFMwPyCjY4Tpeol1gSCrbjDy[ZNxd26|ZTD0c{BCSlRvMk[zM|JFTy:vZYTmc5JucW5iY3;tZolv[XSrb36= NYnyPI5URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PVEyPDVpPkKzOlkyOTR3PD;hQi=>
MCF-7  MX3BdI9xfG:|aYOgRZN{[Xl? Mn;aNlDPxE4EoB?= NH\T[pM4Omh? NV65SGJlcW6qaXLpeJMh\XG3b3ytJIFv\CB2LV;IWE1u\WSrYYTl[EBieG:ydH;zbZM> M4fmbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nke1OlQ{Lz5{M{[3OVY1OzxxYU6=
hCMEC/D3  Mln5RZBweHSxc3nzJGF{e2G7 MYGyOe69VQ>? MV:3Nog> NIXrd5Nz\WS3Y3XzJGx1gEFiaX7keYNm\CCjcH;weI9{cXN? M2S2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nk[1NVk5Lz5{M{[2OVE6QDxxYU6=
Jurkat NXyxfmRGSXCxcITvd4l{KEG|c3H5 M4m2VFEzNjVvNUFOwG0> M{C4dVFp M2C4VoRwe2ViZHXw[Y5l\W62bImgd5VxeHKnc4Pld{BUWEhvaX7keYNm\CCSYYKtOEBkdGWjdnHn[UwhWEGUUDDjcIVifmGpZTygSG5CKG[{YXft[Y51[XSrb36sJIFv\CCub4PzJI9nKH[rYXLpcIl1gQ>? NWPwOo11RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFI6PzZpPkKzOFQzQTd4PD;hQi=>
CNE-1 NGTqcHRCeG:ydH;zbZMhSXO|YYm= NX\NUGc3OjEQvF5CpC=> NUSzXmFTOjSq NGT3eo5qdmirYnn0d{BTSURyMEGtbY5lfWOnZDDj[YxtKGSnYYTo MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR{Nki1NEc,OjN2Mk[4OVA9N2F-
HONE-1 MVHBdI9xfG:|aYOgRZN{[Xl? MXWyNO69VcLi MVOyOIg> NH\OSItqdmirYnn0d{BTSURyMEGtbY5lfWOnZDDj[YxtKGSnYYTo NFTXUVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SyOlg2OCd-MkO0NlY5PTB:L3G+
astrocytes cell MUXBdI9xfG:|aYOgRZN{[Xl? MXW0NO69VQ>? MWe2bC=> Mn3ldoVlfWOnczDlZZJtgSCjcH;weI9{cXNiaX7keYNm\CCkeTDzeIF2em:|cH;ybY5m NX3lXYlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVE4PzhpPkKzOFEyPzd6PD;hQi=>
U-937 Mn3lRZBweHSxc3nzJGF{e2G7 NH\wUG8yOM7:TR?= NUD2VHB3OzCvaX6= NX3WXHU3eHKndnXueJMhXE6ILXnu[JVk\WRibnXjdo9xfG:|aYO= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRzMEe0PEc,OjN2MUC3OFg9N2F-
MDA-MB-231 MkPIRZBweHSxc3nzJGF{e2G7 M1zvPVExOM7:TR?= NIjtS44ycA>? MYXpcohq[mm2czDz[Y5{cXSrenH0bY9vKHSxIGTSRWlNKHWyb36gUXRFUCCmb4fuMZJm\3WuYYTpc44> M2rIOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEC4OFI6Lz5{M{SwPFQzQTxxYU6=
HeLa  MoTuRZBweHSxc3nzJGF{e2G7 MlTCNVAx|ryP M3Hic|Jp NVz0XWpD[myxY3vzJGpTWy1zNTDJcoR2[2W|IFHwc5B1d3SrYzDD[YxtKESnYYTo MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN2NEC0OUc,OjN|NESwOFU9N2F-
Ec109 MWDBdI9xfG:|aYOgRZN{[Xl? MlvXNVDPxE1? MYG2bC=> NF\MWIljdG:la4OgZZBweHSxdHnjJINwdWKrbnH0bY9vKG:oIGTheE1UdWGlTkegZY5lKHKjZHnheIlwdg>? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN|OEW2PEc,OjN|M{i1Olg9N2F-
H460  NX;3clhESXCxcITvd4l{KEG|c3H5 MnnjNVDPxE1? NXjlXIhyPmh? NWPFZ4tM[myxY3vzJIFxd3C2b4TpZ{Bkd22kaX7heIlwdiCxZjDUZZQuW22jY163JIFv\CC{YXTpZZRqd25? M3e2WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{O4OVY5Lz5{M{OzPFU3QDxxYU6=
HeLa  NFi3RW1CeG:ydH;zbZMhSXO|YYm= MVK1NO69VQ>? MU[xMlVp MlHLZYJzd2ejdHXzxsBEcGyjbYnkbYEucW6mdXPl[EBieG:ydH;zbZM> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNyM{iwOEc,OjN|MEO4NFQ9N2F-
SK-HEP1 MnrIRZBweHSxc3nzJGF{e2G7 NH\3TXkyODEQvF2= NEXyd4YycA>? M3XaXIlvcGmkaYTzJGNzXDFiYXP0bZZifGWmIHPhd5Bie2VvMzygMVctKC16LDCtPUwh[W6mIIDvcJkpSUSSLYLpZo9{\SlicH;sfY1memG|ZR?= NYSxZXY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFI3PTBpPkKzN|AzPjVyPD;hQi=>
QGY7701 NXPjfml7SXCxcITvd4l{KEG|c3H5 MV6yOe69VQ>? MlLPNU42cA>? MYDpcohq[mm2czDhZ4N2dXWuYYTpc44hd2Zic4XiMWcyKHCqYYPlJIlv\HWlZXSgZpkhTE:[IDugdZVmemOndHnu NWn5UYFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOFAxPjFpPkKzNlQxODZzPD;hQi=>
HepG2 NY\uUWcySXCxcITvd4l{KEG|c3H5 NY[1R5dmOjEQvF2= M4TGNVMxdWmw M{npR4lvcGmkaYTzJJRp\SCnbnjhcoNm\CClZXzsJIRm[XSqIHL5JINwdWKrbnXkJJRz\WG2bXXueEBw\iCjcHnn[Y5qdiCjbnSgWHJCUUx? MmfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{MkSyN|koRjJ|MkK0NlM6RC:jPh?=
U87  M2O4dmFxd3C2b4Ppd{BCe3OjeR?= Ml;XNlXPxE1? NGHubpQzcA>? MkOy[IVkemWjc3XzJIl{d2yrcYXpdol1cWenbnnuJEhKW0xrLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHisJIJ2fCCwb4SgcoVkem:2aXOgZ4VtdCCmZXH0bC=> Ml7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{Mkm2NlYoRjJ|MkK5OlI3RC:jPh?=
HSC-2 MkLqRZBweHSxc3nzJGF{e2G7 NXLRXFRzOjVxNUFOwG0> NVPxN|h2OWh? MkDobY5pcWKrdIOgV24uOzhvaX7keYNm\CCleYTveI95cWOrdIm= MmDnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNUWyOFgoRjJ|MUW1NlQ5RC:jPh?=
HSC-4 M{LiOGFxd3C2b4Ppd{BCe3OjeR?= M{izTFI2NzVyzszN Ml\6NYg> NWnqU4gzcW6qaXLpeJMhW05vM{itbY5lfWOnZDDjfZRwfG:6aXPpeJk> NWTSbFlNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOVUzPDhpPkKzNVU2OjR6PD;hQi=>
CL-1 NHnJcI1CeG:ydH;zbZMhSXO|YYm= MX:yNO69VQ>? MYSxbC=> NGfKcmxjdG:la4OgU3A1PDliaX7keYNm\CClZXzsJIRm[XSq MnX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzM{G3PFIoRjJ|MUOxO|gzRC:jPh?=
MEL MkG4RZBweHSxc3nzJGF{e2G7 NVLp[FM6OjEQvF2= MlrrNlRp M3TZTmROW09? NX;NNo9LcW2yYXny[ZMhWDK[Nz3pcoR2[2WmIF3FUEBk\WyuIHHwc5B1d3Orcx?= NFnFTmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxOFg5Pyd-MkOwNVQ5QDd:L3G+
Bel-7402  MlTlR4F{eGG|ZTDBZ5RqfmG2aX;uJGF{e2G7 M2\DV|Ux|ryP MYWybC=> MVLpcohq[mm2czDQR2UucW6mdXPl[EBidm:ra3nz NX\zTmJ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFg4PDJpPkKzNFA5PzR{PD;hQi=>
Eca-109 NH31VlhCeG:ydH;zbZMhSXO|YYm= M33OeFI2|ryP M{f6VFMxdWmw Mlr3bY5pcWKrdIOgRmouSjFzLXnu[JVk\WRiY3XscEBl\WG2aB?= NV7MPYx6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|Y6PjdpPkKzNFc3QTZ5PD;hQi=>
MEL NGfDdpJCeG:ydH;zbZMhSXO|YYm= MWmyNO69VQ>? M13xTlFp Mn[2SG1UVw>? MU\pcZBicXKnczDQNng4NWmwZIXj[YQhVUWOIHPlcIwh[XCxcITvd4l{ NEfCNoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxOFg5Pyd-MkOwNVQ5QDd:L3G+
Bel-7402  NGTRVWpCeG:ydH;zbZMhSXO|YYm= M{XndFUx|ryP MV[ybC=> NWjNU2RucW6qaXLpeJMhWEOHLXnu[JVk\WRiYX7vbYtqew>? MkfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEi3OFIoRjJ|MEC4O|QzRC:jPh?=
L929 MVfGeY5kfGmxbjDBd5NigQ>? NUP3SodTOi53zszN MYGxbC=> MUPpcoNz\WG|ZYOgVmlROSCneIDy[ZN{cW:wIHHu[EBmgGGlZYLiZZRm\CCWTldOtU1qdmS3Y3XkJI1qfG:laH;u[JJq[WxiZInz[pVv[3Srb36gZY5lKFKRUzDwdo9lfWO2aX;u MlTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEC1NVgoRjJ|MECwOVE5RC:jPh?=
RCC  M2jPVWNmdGxiVnnhZoltcXS7IFHzd4F6 MUixNFDPxE1? NIHNV2kzPGh? M3nL[JJm[2:4ZYLld{B1cGVidnnhZoltcXS7IH;mJINmdGy|IHX4dI9{\WRidH:gNVVlNVCJSkK= NIjiPHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm5NVQ6PCd-MkK5PVE1QTR:L3G+
NB2a/d1 NXnx[JI6SXCxcITvd4l{KEG|c3H5 M1S5TlExOM7:TR?= M2HiRVczcA>? NWLpVWs1[XS2ZX71ZZRmeyC|dHH1do9{eG:{aX7lMYlv\HWlZXSgZ4F{eGG|ZTDhZ5Rqfmm2eTygVGFTWCxiYX7kJJRifSClbHXheoFo\Q>? NXnCW2dPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5PFg2PDFpPkKyPVg5PTRzPD;hQi=>
T cell NHz3c4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnvdoNjOjVvMUCw{txO NFvHRW4zPGh? MnHi[I9{\S2mZYDlcoRmdnSueTDpcohq[mm2ZXSgWEBk\WyuIIDyc4xq\mW{YYTpc44hdWWmaXH0[YQhfGi{b4XnbEB1cGViY3:td5RqdXWuYYTpc44hf2m2aDDhcpRqNUOGMzDhcoQh[W62aT3DSFI5 M{DUT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUiyOVM5Lz5{Mkm4NlU{QDxxYU6=
K562 MXvBdI9xfG:|aYOgRZN{[Xl? NEL2PYEyODEQvF2= NITiXGQycA>? NHvHV5BjdG:la4OgRYJvd2KjdnnzZ5VuyqCILXnu[JVk\WRiYYDvdJRwe2m| NYf4VJp{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5O|I{PzJpPkKyPVczOzd{PD;hQi=>
Jurkat  NHjqeFRCeG:ydH;zbZMhSXO|YYm= NETLfow1OM7:TR?= NGjicmkycA>? MXPhZo9tcXOqZYOgSoF{VC2rbnT1Z4VlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> MmDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NEK3N|goRjJ{OUSyO|M5RC:jPh?=
BGC-823 NGPUVFRCeG:ydH;zbZMhSXO|YYm= M2\JTFExOM7:TR?= MX[xbC=> MVrwZZJ1cWGubImgdoV{[3WnczDj[YxteyCjZ3HpcpN1KGSjbXHn[UBw\iCmYXnkfoVqdg>? MkHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7Mk[1OFUoRjJ{OUK2OVQ2RC:jPh?=
Hep3B MkO5RZBweHSxc3nzJGF{e2G7 MknQOVDPxE1? MWOxbC=> NXvwUmZt[myxY3vzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEiHR1Pz NVS4Z5dkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlMyPTRpPkKyPVI{OTV2PD;hQi=>
LLC-PK1 MXvBdI9xfG:|aYOgRZN{[Xl? NH3JW3UzOM7:TR?= NIXBfmMycA>? MXnwdoV3\W62czDjbZNxdGG2aX6tbY5lfWOnZDDk[Ydz[WSjdHnvckBw\iCDdHe1MEBj\WOuaX6tNUwh[W6mIFH0[|EzKHC{b4TlbY5{ NF61PGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki5OlA{Pyd-MkK4PVYxOzd:L3G+
A549 MmDGRZBweHSxc3nzJGF{e2G7 MljLOVDPxE1? Ml\1NYg> MmjiZoxw[2u|IITo[UBDSUlvaX7keYNm\CCjcH;weI9{cXQEoB?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh6N{KxOUc,OjJ6OEeyNVU9N2F-
SGC-7901  MWXBdI9xfG:|aYOgRZN{[Xl? MmT0NVDPxE1? MXuyOIg> NITyZXlqdmirYnn0d{DPui{Qsj3kbY1mfGi7bHHjdplte2ira3;ubY4ucW6mdXPl[EBieG:ydH;zbZPDqA>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh2OEW5O{c,OjJ6NEi1PVc9N2F-
DM6 NFjWZpFCeG:ydH;zbZMhSXO|YYm= NIf6cXcyODEQvF2= MkmxO|Jp NGXmdmJjdG:la4OgZo91cCCHMl[tNUBidmRiRULGeJIudWWmaXH0[YQh[3m2b4TvfIlkcXS7 MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NUOyPEc,OjJ6MkWzNlg9N2F-
MCF-7, MDA-MB-468, Caco-2 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfkNJF2PTEQvF2= MmHDOFhp NGXBWIxqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBqdmS3Y3XkJIJ6KHOjcH;ubY4> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhyMEm2PEc,OjJ6MEC5Olg9N2F-
A2750  MnO1RZBweHSxc3nzJGF{e2G7 NX;EfWZ5OjEQvF2= NX7HfGk4Omh? MlHlSG1UVw>? M1f6UYJtd2OtczDjZZNx[XOnIHPs[YF3[WenIHT1dolv\yCqZXzlcoFtcW5idILlZZRu\W62IHHu[EBz\WS3Y3XzJIF2fG:yaHHnbYMh[2WubDDk[YF1cA>? NYD0VYVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3PFQ{PjNpPkKyO|g1OzZ|PD;hQi=>
U87  MnXsRZBweHSxc3nzJGF{e2G7 NYLqbIdDOjEQvF2= M3y3NlI1cA>? NFPKVpNz\WS3Y3XzJJRp\SCjcH;weI9{cXNicnH0[UBqdmS3Y3XkJIJ6KFCOQVK= MlSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{i3PFAoRjJ{N{e4O|gxRC:jPh?=
HT1080 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXPOVDPxE1? MYC3[C=> Ml7MbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnubIljcXSrb36gZ4F2e2WmIHL5JINwdWKrbnXkJJRz\WG2bXXueEBw\iCGQ1GgZY5lKE:PUB?= NEmwbG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0NFk5PCd-MkK3OFA6QDR:L3G+
A549  NF7qXGRCeG:ydH;zbZMhSXO|YYm= M4jWXFUx|ryP NHHxb|EzcA>? M2r0WZBienSrYXzsfUBl\WO{ZXHz[ZMhe2:maYXtJJNmdGWwaYTlMYlv\HWlZXSgZZBweHSxc3nz MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd{MUiwOEc,OjJ5MkG4NFQ9N2F-
Primary OPC NX7w[odDSXCxcITvd4l{KEG|c3H5 M4OwdVHPxE1? MYO2bE8zPGh? NUPWT|RPemWmdXPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBm[XKueT2gZY5lKGyjdHWtZZBweHSxc3nzM45m[3Kxc3nz NVTueY1wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NFc{QDVpPkKyO|A4Ozh3PD;hQi=>
PMNs NF3XN3RCeG:ydH;zbZMhSXO|YYm= M3L5Z|Qx|ryP NEH4[3g3cA>? M3nqWWROW09? MWLy[ZZmenOnZDD0bIUh[W2xdX70JI9nKGOuZXH2[YQh[2G|cHHz[U0{KHSxIH7lZZIhfmWqaXPs[UBt\X[nbIO= MlfzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4OUK1O|coRjJ{NkmyOVc4RC:jPh?=
A549  MV3BdI9xfG:|aYOgRZN{[Xl? M{LSb|Ux|ryP MnjDNYg> NWL5TXFneHKndnXueJMh[XCxcITvd4l{KGmwZIXj[YQh[nliQnHvbJVwe2mmZTDJ M{K3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Nki3OlM2Lz5{Mk[4O|Y{PTxxYU6=
AGS MVfBdI9xfG:|aYOgRZN{[Xl? NUOwcJZtOjEQvF2= MlHHNVJp M{nqb4lvcGmkaYTzJJRp\SCjY4TpeoF1cW:wIH;mJJBzdy2lYYPwZZNmNTNiaX6gdoV{eG:wc3WgeI8hfGinIFX0U2FkKG[{YXP0bY9v NFnscnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[4O|M6QCd-MkK2PFc{QTh:L3G+
shC9  M{jpZWFxd3C2b4Ppd{BCe3OjeR?= MkTlNVDPxE1? NVeyNFFvOT[q M2m3TIJtfW62czDTTGgh\XiycnXzd4lwdiCrbjDzbGM6KGOnbHzzJIV5eG:|ZXSgeI8h\Wm2aHXyJHBCKG:{IFzQRy=> M{T6UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkSxNFk1Lz5{Mk[0NVA6PDxxYU6=
primary MEFs Mn3qSpVv[3Srb36gRZN{[Xl? MnvYNVJp NV:3elc1yqCrbnPy[YF{\XNiZYTvdI9{cWSnLXnu[JVk\WRibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBl\XCxbHHybZpifGmxbh?= NYTiVYtnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVM4PjdpPkKyOlE{PzZ5PD;hQi=>
3T9 MEFs MWTGeY5kfGmxbjDBd5NigQ>? MXexOog> MWfpcoNz\WG|ZYOgZ5l1d2Oqcn;t[UBkKHKnbHXhd4Uh[W[2ZYKg[ZRweG:|aXTlMZRz\WG2bXXueC=> M1zWZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkGzO|Y4Lz5{Mk[xN|c3PzxxYU6=
3T9 MEFs MojmSpVv[3Srb36gRZN{[Xl? NF;qV5QyQGh? MlTKeZBz\We3bHH0[ZMhcW6rdHnheI9zKGOjc4Dhd4UuQSCkdYSg[I94dnKnZ4XsZZRmeyCnZn\lZ5RweiClYYPwZZNmeyCjZoTldkBmfG:yb4Pp[IUhfHKnYYTt[Y51 MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzM{e2O{c,OjJ4MUO3Olc9N2F-
MDA-MB-231  M{TJSGFxd3C2b4Ppd{BCe3OjeR?= MYGyNO69VQ>? MmqwOIQ> NI\YcYtz\WS3Y3XzJJRp\SClZXzsJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KG2ndH\vdo1qdiC2cnXheI1mdnRid3HzJJNq\26rZnnjZY51dHl? MlPSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OUO0OFEoRjJ{NUmzOFQyRC:jPh?=
C6  NF;RfW9CeG:ydH;zbZMhSXO|YYm= M4P0V|Ex|ryP M1HEfVI1cA>? NWPY[JQy[myxY3vzJJRp\SC|dYDwdoV{e2m4ZTDl[oZm[3Rib3[geIhmKHCncITp[IUhd25idnnhZoltcXS7 NHnOWFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4PFk5OCd-MkK1PFg6QDB:L3G+
HL-60  MWDBdI9xfG:|aYOgRZN{[Xl? MmPlNVAx|ryP M{\FW|I1cA>? M{HuUolvcGmkaYTzJJRp\SClZXzsJIFxd3C2b4Ppd{B{\WynY4Tl[EApMylvbXXueIh6dCEQsj2oNgKHmjZrLXTp[4x2[2:yeYLhco9{cWSnwrC1 NGrM[VA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0OlY3QSd-MkK1OFY3Pjl:L3G+
HL-60 M2\rbWFxd3C2b4Ppd{BCe3OjeR?= NU\iXnlvOTBvOEFOwG0> MX20bC=> MV\pcohq[mm2czDUS2hSNWmwZIXj[YQh[2WubDDhdI9xfG:|aYRCpC=> NWTKXIV[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NlMzOjlpPkKyOVI{OjJ7PD;hQi=>
BCC NEfJU5pCeG:ydH;zbZMhSXO|YYm= NHfjdZA2OM7:TR?= NYLrW41TOWh? MkHTbY5pcWKrdIOgSGFVWy2vZXTpZZRm\CCpcn;3eIghcW6qaXLpeIlwdg>? NFrpbWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxPVQ{Pid-MkK1NVk1OzZ:L3G+
RAW 264.7 M3HCXGFxd3C2b4Ppd{BCe3OjeR?= MUe1NE8yODEQvF2= NVHybYRkOWh? NIrGVXZkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcohq[mm2czDEU24ucW6mdXPl[EBzWk6DIHPs[YF3[Wen MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR7MUSyOkc,OjJ2OUG0NlY9N2F-
K562 M1joemFxd3C2b4Ppd{BCe3OjeR?= Mn3CNlXPxE1? MVOybC=> M1roOJBz\X[nboTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGOxLYTy[YF1dWWwdDD3bZRpKGGvdYLlcpNqdiCJIHHu[EBVWkGLTB?= NHrD[o89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4N|c4Pyd-MkK0PFM4Pzd:L3G+
SGC-7901 Mlf6RZBweHSxc3nzJGF{e2G7 NFL1WoUzOCEQvF5CpC=> MmDLNog> NUjXOYcx[XS2ZX71ZZRmeyCKMl:yxsBweiCWTl[g{tEucW6mdXPl[EBk\WyuIHHwc5B1d3OrczDhd{B4\WyuIHHzJINie3Cjc3WtN{Bi[3Srdnn0feKh Mo\HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2N{G1PFkoRjJ{NEexOVg6RC:jPh?=
PC3 NG[2ZXVCeG:ydH;zbZMhSXO|YYm= NIrh[ZoyOM7:TR?= NEjZWHk1cA>? NVyzSIJm[2:3boTldpMh\myjdn;jc5hq\C2rbnT1Z4VlKGOjc4Dhd4UuemWuYYTl[EBieG:ydH;zbZM> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR5MUm3OEc,OjJ2N{G5O|Q9N2F-
SMMC-7721 MXLBdI9xfG:|aYOgRZN{[Xl? NF;6eoM2OM7:TR?= M1rteFQ5cA>? NIDPNFRifHSnboXheIV{KG:WUj3pcoR2[2WmIHHwc5B1d3Orcx?= MlL1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkW4N|MoRjJ{NE[1PFM{RC:jPh?=
HeLa  NYqwdGx1SXCxcITvd4l{KEG|c3H5 M3Xad|UxyqEQvF2= MWG0M|hp M3vNZ4lvcGmkaYTzJHNVWy2rbnT1Z4VlKGyjdHWtdIhie2ViYYDvdJRwfGmlIHX2[Y51ew>? M1rN[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[wOVA1Lz5{MkS2NFUxPDxxYU6=
HeLa  MXXBdI9xfG:|aYOgRZN{[Xl? NYXVbpY2PTEEoN88US=> NIP4XJIycA>? Mmf5d5VxeHKnc4Pld{B1cGViRmLBVE1qdmS3Y3XkJIFk[3WvdXzheIlwdiCxZjDhco5mgGmwIG[gdI9{cXSrdnWgZ4VtdHN? MlToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NEm0OFAoRjJ{NES5OFQxRC:jPh?=
T47D  Mnf1RZBweHSxc3nzJGF{e2G7 MWqxNFDPxE1? NGPnemUycA>? MWjicI9kc3NidHjlJIdmdmW{YYTpc44hd2ZiRT3jZYQwS1SIMjDifUBUXFN? NYi4V5JjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NFEyPjhpPkKyOFAyOTZ6PD;hQi=>
HeLa  M4W5fmFxd3C2b4Ppd{BCe3OjeR?= MYCzNO69VQ>? MYq0bC=> MX9CpIlv[3KnYYPld{B1cGViZ3Xu[ZJidCClZXzsJJZq[WKrbHn0fUA1QCCqIHHmeIVzKHCqb4Tv[Jlv[W2rYzD0doVifG2nboVCpC=> MkfFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|OUSyOFgoRjJ{M{m0NlQ5RC:jPh?=
HCC M3fRZ2Fxd3C2b4Ppd{BCe3OjeR?= M1OyXFIxKM7:TdMg M{HYdlJp NXvxSGlt[XS2ZX71ZZRmeyC2aHWgSGhCKGmwZIXj[YQh[WO2aY\heIlwdiCxZjDQRXJR MmjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NEK3N|IoRjJ{M{SyO|MzRC:jPh?=
mESCs MnS5RZBweHSxc3nzJGF{e2G7 NV3qcY1lOi53zszN NUXBXpBoOmh? NWHTb2ZFcW6qaXLpeJMhfGinIF7hSk1u\WSrYYTl[EBk[XOyYYPlJIFkfGm4YYTpc44> NIHhVnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK4OVI4PCd-MkKyPFUzPzR:L3G+
EMT-6  NYjXenpwS3m2b4TvfIlkcXS7IFHzd4F6 MnPiNVAx|ryP NYTnU|h[OWh? NEHvXm1x[XK2aXHscJkh[myxY3vl[EBk\WyuIHTlZZRpKGmwZIXj[YQh[nlic3nyZY1me2mwZR?= Mli5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NUG5NlEoRjJ{MkWxPVIyRC:jPh?=
MCF7 NIX5PFlCeG:ydH;zbZMhSXO|YYm= M1G2PVUxyqEQvF2= NHTCfJMycA>? MnPybY5pcWKrdIOgVGEh[WO2aY\heIVlKGOjc4Dhd4UuOyxiLUmsJIFv\CCyb3z5LGFFWC2{aXLvd4UqKHCxbInt[ZJie2V? MlHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MkOzOFUoRjJ{MkKzN|Q2RC:jPh?=
K562 MXLBdI9xfG:|aYOgRZN{[Xl? Mn\BNlAh|ryPwrC= MnfjOFhp MljrSG1UVw>? NWnlO4VP[myxY3vzJIxieGG2aX7pZk1qdmS3Y3XkJIlvcGmkaYTpc44hd2ZidnnhZoltcXS7IHHu[EBieG:ydH;zbZMhcW6mdXP0bY9v MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJzNkG1PEc,OjJ{MU[xOVg9N2F-
Molt4-hyg NV;FZW1MSXCxcITvd4l{KEG|c3H5 NF\CWZUyOM7:TR?= NXrUOHdrOC53aB?= MkjuZoxw[2u|IH\hdo5me2:uLXnu[JVk\WRiY3HzdIF{\S1|LXzpb4Uh[WO2aY\peJk> Mo\GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{W4NVEoRjJ4Mke1PFEyRC:jPh?=
HeLa M3zGbWFxd3C2b4Ppd{BCe3OjeR?= NVHFTGVGOTEQvF2= NYrRPFV7OC53aB?= NXrUc3B1cW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nlidHjlJINwdWKrbnXkJJRz\WG2bXXueEB4cXSqIHfvcYl{cW5iTjDhcoQhXFKDSVy= NVPqWHcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxO|k3PjFpPkKyNVc6PjZzPD;hQi=>
Jurkat T M4DwcGFxd3C2b4Ppd{BCe3OjeR?= MXuzNO69VQ>? MkDyNE42cA>? MVnEUXNQ MWXicI9kc3NidHjlJJpqemGvLXnu[JVk\WRiYYDvdJRwe2m| MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF3OUi5PEc,OjJzNUm4PVg9N2F-
Neutrophil MVPBdI9xfG:|aYOgRZN{[Xl? M{XQWlIx|ryP M2TsPVAvPWh? M4HtbYF1fGWwdXH0[ZMhfGinIIDyc{1ieG:ydH;0bYMh\W[oZXP0JI9nKE2jUkG= NYW4WVVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVY5PDRpPkK2NVk3QDR2PD;hQi=>
HCT116 MnvQRZBweHSxc3nzJGF{e2G7 MlPaOVDDqM7:TR?= NX7KWZpbOmh? MWDy[ZZmenOnczDzfY5memerc4TpZ{BieG:ydH;zbZMh\W[oZXP0d{Bw\iClZXzlZ495cWJiYX7kJG5RSy1zNtMg MorGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzNUm3OVIoRjJ{MUW5O|UzRC:jPh?=
MDA-MB-231  MVLBdI9xfG:|aYOgRZN{[Xl? MWSyMlUuPy53zszN NEXxbI4zcA>? NUPMXphEcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigc4YhVUSDLV3CMVI{OSClZXzsd{BqdmS3Y3XkJIJ6KFOGVDDpckBiKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70JI1idm6nch?= M1XkOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUG1OVI3Lz5{MkGxOVUzPjxxYU6=
LNCaP NFjzWWRCeG:ydH;zbZMhSXO|YYm= NUTQbWN2PDEQvF2= NXvYcpVWOmh? MW\pcohq[mm2czDieZRmcW5iaX7keYNm\CClZXzsJIFxd3C2b4Ppdy=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzNEe2OEc,OjJzMUS3OlQ9N2F-
MB231 MXLBdI9xfG:|aYOgRZN{[Xl? M3;HcFExOM7:TR?= NHrWdpUycA>? MkLtSG1UVw>? M3n1fYFjem:pYYTld{B1cGViaX7keYN1cW:wIH;mJINmdGxiZHXheIgh[nliV1XFNUBqdmirYnn0bY9vNCCWUlHJUEB1emWjdH3lcpQtKGGwZDD0bIUh[2:vYnnuZZRqd25? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzMkm0NEc,OjJzMUK5OFA9N2F-
HCC38  NYG2VnhmSXCxcITvd4l{KEG|c3H5 MkjqNVAx|ryP NG\Xe40ycA>? NXrvU4hpTE2VTx?= Mn7RZYJzd2ejdHXzJJRp\SCrbnT1Z5Rqd25ib3[gZ4VtdCCmZXH0bEBjgSCZRVWxJIlvcGmkaYTpc44tKFSUQVnMJJRz\WG2bXXueEwh[W6mIITo[UBkd22kaX7heIlwdg>? NIjYe|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGxNlk1OCd-MkKxNVI6PDB:L3G+
MDA-MB231 MoLxRZBweHSxc3nzJGF{e2G7 NVG5ZWR4PTEEoN88US=> NV6wR3hIOjSq MVrEUXNQ MnvZZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFyMUOzOUc,OjJzMEGzN|U9N2F-
LNCaP M4TKUWFxd3C2b4Ppd{BCe3OjeR?= MnLPOVDDqM7:TR?= NUHCfpRuOjSq MWrEUXNQ NWfFSZdj[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MlvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMEGzN|UoRjJ{MUCxN|M2RC:jPh?=
HCT116 M3fHU2Fxd3C2b4Ppd{BCe3OjeR?= NVnMVHF5PTEEoN88US=> MW[yOIg> NULzW4pYTE2VTx?= MU\hZpJw\2G2ZYOgZ5Vz[3WvaX6tbY5lfWOnZDDj[YxtKGSnYYTo MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFyMUOzOUc,OjJzMEGzN|U9N2F-
Ishikawa  NGfzfnpCeG:ydH;zbZMhSXO|YYm= NFTRbJozPc7:TR?= NUL6clFNOjSq NILWVFNz\WS3Y3XzJINmdGxiZHXheIgh[W6mIHHwc5B1d3OrczDpcoR2[2WmIHL5JGJi\iCDMR?= M{GweFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEi4PVE5Lz5{MkC4PFkyQDxxYU6=
 YD-8  MX\BdI9xfG:|aYOgRZN{[Xl? MkDlNVAx|ryP Mni4NYg> MojBbY5pcWKrdIOgVGxGVy2rbnT1Z4VlKGGyb4D0c5Nqew>? MnTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyOE[xPFMoRjJ{MEi2NVg{RC:jPh?=
eosinophil  Ml3IRZBweHSxc3nzJGF{e2G7 NEfmVXE6OM7:TR?= M{OwbFI1cA>? MWfpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBH[XNiYX70bYJw\HoEoB?= MmLSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{mzN|QoRjJ{MEe5N|M1RC:jPh?=
L929 M2nycmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTONnEzNjYQvF2= NH3WUYczPGh? MWDpcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjDwMZA{QCCjbnSgUmYu|rqEIITvJIF2\22nboSgWG5H|rFvaX7keYNm\CCwZXPyc5B1d3OrczDhcoQh[XW2b4DoZYd6 MmHsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkewPVcoRjJ{MEK3NFk4RC:jPh?=
YD-8  NWrEV256SXCxcITvd4l{KEG|c3H5 M1TQc|ExOM7:TR?= NVPzT2JDOWh? M3XudIJtd2OtczD0bIUhT1NvSFPsMYlv\HWlZXSgZZBweHSxc3nz M3;zWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKwNFc5Lz5{MkCyNFA4QDxxYU6=
HBx MX7BdI9xfG:|aYOgRZN{[Xl? NELOTnAzPc7:TR?= M1jZdFQ5cA>? NVTpe25xTE2VTx?= NW\qSXlCemWmdXPl[EBk\WyuIHTlZZRpKGmwZIXj[YQh[nliMz3NRS=> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{MEC3PEc,OjJyMkCwO|g9N2F-
U937  MlfWRZBweHSxc3nzJGF{e2G7 MoDKOVDDqM7:TR?= NV3COXd3OWh? NWT1e4pKcW6qaXLpeJMhUEZvaX7keYNm\CCjcH;weI9{cXQEoB?= NUezfHI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5PVg4OzFpPkKxPVk5PzNzPD;hQi=>
HL60 NYT0R41KSXCxcITvd4l{KEG|c3H5 NETUWHQ1OM7:TR?= NFXJRY01OG2rbh?= MWTEUXNQ MnjjZoxw[2u|IFLOSGMh[2:vcH;1coR{KGmwZIXj[UBmgHCxc4Xy[UBw\iCyaH;zdIhifGmmeXzz[ZJqdmViYX7kJGRPSSCocnHncYVvfGG2aX;u MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl6M{K5Okc,OjF7OEOyPVY9N2F-
M-14 NHTmTHFCeG:ydH;zbZMhSXO|YYm= NILMO3ozPc7:TR?= Mmi3NE42cA>? MYNCpIlvcGmkaYTzJIJwfGhidHjlJINzfWSnIHX4eJJi[3RvIHHu[EBkd22yb4Xu[E1qdmS3Y3XkJIFxd3C2b4Ppdy=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl3NEm1PUc,OjF7NUS5OVk9N2F-
SK-BR-3 NVzGPI1kSXCxcITvd4l{KEG|c3H5 M{nM[VUxyqEQvF2= NEX5[HkzcA>? M4[4U4Jtd2OtczDhdI9xfG:2aXOgSG5CKG[{YXft[Y51[XSrb36gbY5lfWOnZDDifUBkd22kaX7heIlwdiCxZjDTUU0yPjRiYX7kJJJi\GmjdHnvci=> M3n6NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUCxN|g3Lz5{MUmwNVM5PjxxYU6=
MDA-MB-468 M{\RbmFxd3C2b4Ppd{BCe3OjeR?= MlTmOVDDqM7:TR?= M2PHUFJp MlqzZoxw[2u|IHHwc5B1d3SrYzDEUmEh\nKjZ33lcpRifGmxbjDpcoR2[2WmIHL5JINwdWKrbnH0bY9vKG:oIGPNMVE3PCCjbnSgdoFlcWG2aX;u MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlyMUO4Okc,OjF7MEGzPFY9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3; 

PubMed: 27191889     


With the addition of Z-VAD-FMK, levels of cleaved Caspase-3 and PARP1 were decreased.

Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II; 

PubMed: 27191889     


Levels of Beclin1, Atg5, Atg7 and LC3-II were decreased after IFN-γ stimulation. However, when IFN-γ was combined with the caspase inhibitor, levels of Beclin1, Atg5, Atg7 and LC3-II were not decreased as determined by the Western Blot test. 

p23 / Hsp90 / hTRET / MMP2; 

PubMed: 19740745     


Expression of Hsp90 client proteins during cell death. HeLa cells were preincubated with z-VAD-fmk (100 μM) for 1 h and exposed to various concentrations of tunicamycin (Tu, in μM) for 24 h. Cell extracts were prepared and analyzed by Western blotting. β-Actin was used as the loading control.

cyto C; 

PubMed: 17301139     


z-VAD-fmk did not inhibit cytochrome c release in RRV-infected MA104 cells (RRV: rhesus rotavirus). Mock- and RRV-infected MA104 cells either were not pretreated or were pretreated with 100 μM z-VAD-fmk for 2 h before RRV infection, and treatment was continued during infection. Fourteen hours after RRV infection, the cytosolic and heavy-membrane fractions were assayed for cytochrome c by Western blotting. Mock-infected MA104 cells were used as negative controls. Cox IV and tubulin were used as protein loading controls for the heavy-membrane and cytosolic fractions, respectively. Positions of molecular weight markers are indicated on the left.

27191889 19740745 17301139
Growth inhibition assay
Cell viability; 

PubMed: 12522090     


Inhibition of magnolol-induced apoptotic cell death by z-VAD-fmk. CH27 cells were treated with cell permeable broad-spectrum caspase inhibitor (z-VAD-fmk) 2 h prior to 80 μm magnolol treatment. Cell viability was estimated. ***P<0.001, compared with the 80 μM magnolol-treated group. 

12522090
Immunofluorescence
cytochrome c ; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Smac/DIABLO; 

PubMed: 11726499     


Immunolocalization of Smac/DIABLO and cytochrome c in HeLa cells. (A-C) Viable (non-apoptotic) HeLa cells were stained with antibodies to cytochrome c (green) and Smac/DIABLO (red) as indicated. Note that both proteins clearly co-localize within punctate, mitochondrial structures. (D–F) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were UV-irradiated followed by further incubation for 8 h. Note the cells where cytochrome c has exited mitochondria and can be seen in a diffuse pattern throughout the cell, including the nucleus (white arrows). In the latter case, note the retention of Smac/DIABLO in punctate mitochondrial structures within the cytoplasm. (G-I) HeLa cells, pre-incubated with z-VAD-fmk (100 µM), were then treated with 10 µM actinomycin D for 8 h. Once again note the retention of Smac/DIABLO in mitochondrial structures. (J-L) HeLa cells were treated as described in (G-I). A close-up of a single cell that has undergone mitochondrial cytochrome c release, but where Smac/DIABLO is retained within mitochondria, is shown.

Endo G; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of EndoG together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

AIF; 

PubMed: 12941691     


GFP-Bax expression and immunostaining of AIF together with nuclear Hoechst staining in HeLa cells 18 h after transient transfection with a vector encoding GFP-Bax in the absence or in the presence of the caspase inhibitor z-VAD-fmk (100 μM). 

11726499 12941691
ELISA
IL-1β; 

PubMed: 26254306     


Cell-free medium supernatants were harvested at 5 h. IL-1β levels were measured by ELISA. Z-VAD-FMK was used as a positive control. Bars represent means ± sem of pooled results from 3 experiments performed in triplicate at different times.

26254306
In vivo

In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo[7].

Protocol

Cell Research:

[6]

- Collapse
  • Cell lines: human granulosa cell lines (GC1a, HGL5, COV434)
  • Concentrations: 50 μM
  • Incubation Time: 48 h
  • Method:

    To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h with etoposide (50 μg/ml) and/or Z-VAD-FMK (50 μM) under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: CD1 mice
  • Dosages: 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (198.93 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+35 %PEG 300+2%Tween 80+ddH2O
For best results, use promptly after mixing.
6mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage powder
in solvent
Synonyms Z-VAD(OMe)-FMK
Smiles CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Can you suggest an in vivo formulation of S7023 for animal studies?

  • Answer:

    For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

  • Question 2:

    What is difference between S7023 & S8102?

  • Answer:

    Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

Caspase Signaling Pathway Map

Related Caspase Products

Tags: buy Z-VAD-FMK | Z-VAD-FMK supplier | purchase Z-VAD-FMK | Z-VAD-FMK cost | Z-VAD-FMK manufacturer | order Z-VAD-FMK | Z-VAD-FMK distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID