740 Y-P (PDGFR 740Y-P)

Catalog No.S7865 Synonyms: 740YPDGFR

For research use only.

740 Y-P (PDGFR 740Y-P, 740YPDGFR) is cell-permeable phosphopeptide activator of PI3K.

740 Y-P (PDGFR 740Y-P)  Chemical Structure

CAS No. 1236188-16-1

Selleck's 740 Y-P (PDGFR 740Y-P) has been cited by 22 publications

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Biological Activity

Description 740 Y-P (PDGFR 740Y-P, 740YPDGFR) is cell-permeable phosphopeptide activator of PI3K.
PI3K [1]
In vitro

The PDGFR 740Y-P peptide stimulates a mitogenic response in muscle cells. The ability of the 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[2]. 740Y-P is as effective as a growth factor (FGF2) at promoting neuronal cell survival via the established PI 3-kinase-Akt survival cascade. Stimulation of neuronal cell survival by the PDGFR740Y-P peptide is dose dependent and does not require insulin[1].

Protocol (from reference)

Cell Research:


  • Cell lines: NIH 3T3 cells
  • Concentrations: 50 μg/ml
  • Incubation Time: 2 h
  • Method:

    NIH 3T3 cells (2×106) are incubated in suspension at 37°C in Ca2+ and Mn2+ free HBSS, 10% FCS containing either 50 μg/ml of 740Y-P peptide or an equal volume of PBS as a control. After 2h cells are centrifuged, washed and trypsinised to degrade non-internalised peptide. Cells are then resuspended in lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 10% Glycerol, 2% NP40, 0.25% deoxycholate, 1 mM EDTA, 1 mM Vanadate, Protease inhibitors "complete" cocktail from Boehringer-Mannheim) for 1 h at 4°C. Lysates are clarified by centrifugation at 1.4×104g for 5 min and the supernatants are incubated for 1h with streptavidin-agarose beads. Beads are subsequently washed three times in lysis buffer and finally boiled in SDS sample buffer and resolved by SDS-PAGE on a 12% gel, transferred to nitrocellulose and immunoblotted with the p85 monoclonal antibody.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.


Chemical Information

Molecular Weight 3270.70


CAS No. 1236188-16-1
Storage 3 years -20°C powder
2 years -80°C in solvent

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