Fer-1 (Ferrostatin-1)

Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.

Fer-1 (Ferrostatin-1) Chemical Structure

Fer-1 (Ferrostatin-1) Chemical Structure

CAS: 347174-05-4

Selleck's Fer-1 (Ferrostatin-1) has been cited by 393 publications

Purity & Quality Control

Batch: Purity: 99.98%
99.98

Products often used together with Fer-1 (Ferrostatin-1)

RSL3


Ferrostatin-1 and RSL3 combination significantly inhibit NCX4040 cytotoxicity in colorectal cancer (CRC) cells, HT-29/HCT 116.


Sinha BK, et al. Cells. 2023 Jun 14;12(12):1626.

Z-VAD-FMK


Ferrostatin-1 and Z-VAD-FMK combination block the cell viability inhibited by CAY10566 in ovarian cancer cell lines OVCAR-5.


Zhao G, et al. Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2203480119.

Obeticholic Acid (INT-747)


Ferrostatin-1 and Obeticholic acid combination significantly reduce inflammation in non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) disease.


Li S, et al. Front Pharmacol. 2022 Dec 16;13:1081553.

Chloroquine


In the presence of nanoplatform, Ferrostatin-1 has no effect on cell growth, while Chloroquine alleviates growth inhibition in H460/A549 cells.


Liu R, et al. J Exp Clin Cancer Res. 2023 Jul 29;42(1):188.

Cisplatin


Ferrostatin-1 and Cisplatin combination are used to rescue selenium-induced eye defects.


Gao M, et al. Int J Mol Sci. 2022 Apr 26;23(9):4783.

Fer-1 (Ferrostatin-1) Related Products

Choose Selective Ferroptosis Inhibitors

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human IMR32 neuroblastoma cells Function assay 13 h Antiferroptotic activity in human IMR32 neuroblastoma cells assessed as inhibition of erastin-induced cell death preincubated for 1 hr before erastin stimulation and measured after 13 hrs by fluorescence plate reader method, IC50=0.018 μM 26696014
HT1080 cells Function assay Antiferroptotic activity in human HT1080 cells assessed as inhibition of erastin-induced cell death by Alamar blue assay, IC50=0.095 μM 26696014
HRE cells Cell viability assay 1 μM 48-72 hours Induction of cell apoptosis 30794682
U57810 and C2C12 cells Cell viability assay 10 μM 24, 48, and 72 h Administration of ferrostatin-1 almost completely prevented the ferroptotic cell death. 29971532
Leishmania major Friedlin clone V1 Antileishmanial assay 72 h LD50 = 28.75 μM 26410073
IMR32 Function assay 1 h IC50 = 0.033 μM 30354101
Click to View More Cell Line Experimental Data

Biological Activity

Description Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.
Targets
Ferroptosis [1]
(HT-1080 cells)
60 nM(EC50)
In vitro
In vitro

Ferrostatin-1 (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. Ferrostatin-1 is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. Ferrostatin-1 does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. [1]

Cell Research Cell lines HT-1080 cells
Concentrations 60 nM (EC50)
Incubation Time 24 h
Method

Erastin treated cells were incubated with ferrostatin-1 at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.

Experimental Result Images Methods Biomarkers Images PMID
Western blot Nrf2 / Gpx4 / β-Actin gp91 phox / β-actin KIM-1 / NGAL / β-actin Collagen I / β-actin cleaved caspase-3 / β-actin 31571665
Growth inhibition assay Cell viability Cell viability 31899616
Immunofluorescence γH2AX morphological features of HT-22 cells renal apoptosis ROS formation 31899616
In Vivo
In vivo

Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.[2]

Animal Research Animal Models C57BL/6 mice
Dosages 10 mg/kg
Administration i.n.

Chemical Information & Solubility

Molecular Weight 262.35 Formula

C15H22N2O2

CAS No. 347174-05-4 SDF Download Fer-1 (Ferrostatin-1) SDF
Smiles CCOC(=O)C1=CC(=C(C=C1)NC2CCCCC2)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 55 mg/mL ( (209.64 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 55 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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