PI-103

Catalog No.S1038

For research use only.

PI-103 is a multi-targeted PI3K inhibitor for p110α/β/δ/γ with IC50 of 2 nM/3 nM/3 nM/15 nM in cell-free assays, less potent to mTOR/DNA-PK with IC50 of 30 nM/23 nM. PI-103 induces apoptosis in murine T-cell Lymphoma.

PI-103 Chemical Structure

CAS No. 371935-74-9

Selleck's PI-103 has been cited by 83 publications

Purity & Quality Control

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Biological Activity

Description PI-103 is a multi-targeted PI3K inhibitor for p110α/β/δ/γ with IC50 of 2 nM/3 nM/3 nM/15 nM in cell-free assays, less potent to mTOR/DNA-PK with IC50 of 30 nM/23 nM. PI-103 induces apoptosis in murine T-cell Lymphoma.
Features The first potent, synthetic mTOR inhibitor.
Targets
p110α [3]
(Cell-free assay)
p110β [3]
(Cell-free assay)
p110δ [3]
(Cell-free assay)
p110γ [3]
(Cell-free assay)
DNA-PK [3]
(Cell-free assay)
Click to View More Targets
2 nM 3 nM 3 nM 15 nM 23 nM
In vitro

PI-103 potently inhibits both the rapamycin-sensitive (mTORC1) and rapamycin-insensitive (mTORC2) complexes of the protein kinase mTOR. [1] PI-103 inhibits constitutive and growth factor-induced PI3K/Akt, as well as mTORC1 activation. [2] In blast cells, PI-103 inhibits leukemic proliferation, the clonogenicity of leukemic progenitors and induces mitochondrial apoptosis, especially in the compartment containing leukemic stem cells. PI-103 inhibits p110α >200-fold more potently than p110β. PI-103 also potently blocks production of PI(3,4)P2 and PIP3 in adipocytes and PIP3 in myotubes. [2] PI-103 inhibits phosphorylation of Akt with an IC95 100-fold lower than that for LY294002. Strikingly, PI-103 completely protects animals from insulin-stimulated decline in blood glucose. PI-103 has additive proapoptotic effects with etoposide in blast cells and in immature leukemic cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
UCH-1  NVu5fJplSXCxcITvd4l{KEG|c3H5 MV6wMlEuOTBizszN M3\mVFI1KGh? M4f5fGROW09? NFiwVZNqdmS3Y3XzJIFxd3C2b4Ppdy=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV{OES0NUc,OTl3Mki0OFE9N2F-
UCH-1  MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3K5elAvODFvMUCg{txO MmK0OkBl M1XTdolvcGmkaYTzJJBzd2yrZnXyZZRqd25iZH;z[UBl\XCnbnTlcpRtgQ>? NF:3NVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWyPFQ1OSd-MUm1Nlg1PDF:L3G+
UCH-1  NVXHcohQTnWwY4Tpc44hSXO|YYm= NH63PHAxNTVizszN NYW3RodLcW6qaXLpeJMhfGinIIDoc5NxcG:{eXzheIlwdiCxZjDic5RpKEGNVDDhcoQhdVSRUjDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M36zVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUK4OFQyLz5zOUWyPFQ1OTxxYU6=
HUVEC M1TiWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzPPVRWUUN3ME2wMlA5KM7:TR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV6NEKyO{c,OTl3OESyNlc9N2F-
SKOV-3 NXHFR45MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrOTWM2OD1yLkGyJO69VQ>? MnTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3OESyNlcoRjF7NUi0NlI4RC:jPh?=
PC3  M17qOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYCxNIFNUUN3ME2wMlExKM7:TR?= MnXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3OESyNlcoRjF7NUi0NlI4RC:jPh?=
DETROIT562 NIT3d4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwMUOg{txO NV;2bpFJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1PFQzOjdpPkG5OVg1OjJ5PD;hQi=>
IGROV-1 NXT4SoN6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkS1TWM2OD1yLkC2JO69VQ>? NITzTHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW4OFIzPyd-MUm1PFQzOjd:L3G+
U87MG M{DrNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3SxTmlEPTB;MD6xOEDPxE1? NEKwWIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW4OFIzPyd-MUm1PFQzOjd:L3G+
U118MG NFXvZ|ZHfW6ldHnvckBCe3OjeR?= NYqyO44{OC5zLUGg{txO M4LoR|I16oDLaNMg MWPEUXNQ NW\MPJFNcW6qaXLpeJMhWEl|Sz3t[YRq[XSnZDDzbYdv[Wyrbne= NHPGXIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[zN|Y5Oyd-MUm2N|M3QDN:L3G+
U138MG M2H5U2Z2dmO2aX;uJGF{e2G7 NWP2UWJrOC5zLUGg{txO NUjQTZRJOjUkgJnoxsA> MVnEUXNQ MnT5bY5pcWKrdIOgVGk{Uy2vZXTpZZRm\CC|aXfuZYxqdmd? MoezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl4M{O2PFMoRjF7NkOzOlg{RC:jPh?=
U87MG M322[GZ2dmO2aX;uJGF{e2G7 NFrUb3cxNjFvMTFOwG0> M3zyflI16oDLaNMg MlPRSG1UVw>? NFG0PXZqdmirYnn0d{BRUTONLX3l[IlifGWmIIPp[45idGmwZx?= M4rTUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NkOzOlg{Lz5zOU[zN|Y5OzxxYU6=
PC3  M4XQXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fmZVI1cA>? M3n6R2dKPTEEoE2gNVAxKG6P NIPCUm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEW1NVA3OSd-MkC1OVExPjF:L3G+
518A2 NGDtSIJHfW6ldHnvckBCe3OjeR?= NGThc|ExNjByMfMAl|HjiIoQvF2= MWWyOEBp MYPzeZBxemW|c3XzJJBpd3OyaH;yfYxifGmxbjDv[kBxcG:|cHjheIllgWxiaX7vd4l1d2xiMz3rbY5ie2ViZH;3cpN1emWjbTD0ZZJo\XS| MoTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyNEi3PFUoRjJzMES4O|g2RC:jPh?=
Mel-Juso  MW\GeY5kfGmxbjDBd5NigQ>? NVXHcI8xOC5yMEJihLMy6oDLzszN NGTpbYozPCCq MWHzeZBxemW|c3XzJJBpd3OyaH;yfYxifGmxbjDv[kBxcG:|cHjheIllgWxiaX7vd4l1d2xiMz3rbY5ie2ViZH;3cpN1emWjbTD0ZZJo\XS| MknYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyNEi3PFUoRjJzMES4O|g2RC:jPh?=
518A2  NH\kR3dE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYnXXWd3OC5yMfMAl|Ex6oDLzszN NFvxOFU4OiCq MknkbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB2OEe4OUc,OjFyNEi3PFU9N2F-
Mel-Juso NUDxOXc3S2WubDDWbYFjcWyrdImgRZN{[Xl? NVPsUpJmOC5yMfMAl|Ex6oDLzszN M1i1UFczKGh? MWLpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M{jaOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMES4O|g2Lz5{MUC0PFc5PTxxYU6=
SF767 NIP3UnJHfW6ldHnvckBCe3OjeR?= NIf5VpUyKM7:TR?= NFzESoM1QCCq NXPLXmdDcW6mdXPld{BifXSxcHjh[49{d22nIH\vdo1ifGmxbh?= NV\kT|VTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwOlI6QTNpPkKxNFYzQTl|PD;hQi=>
U373 M3T1ZWZ2dmO2aX;uJGF{e2G7 Mn7uNUDPxE1? NH7vZmI1QCCq MmS5bY5lfWOnczDheZRweGijZ3;zc41mKG[xcn3heIlwdg>? M4jZe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzME[yPVk{Lz5{MUC2Nlk6OzxxYU6=
U87 NV\nR3ZMTnWwY4Tpc44hSXO|YYm= NWPpV3VOOSEQvF2= NE\F[oM1QCCq NXjiV3RWcW6mdXPld{BifXSxcHjh[49{d22nIH\vdo1ifGmxbh?= NWfibm5RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwOlI6QTNpPkKxNFYzQTl|PD;hQi=>
LN229 MVXGeY5kfGmxbjDBd5NigQ>? NV;2bYkxOSEQvF2= NYHuVlh3PDhiaB?= M{PCcYlv\HWlZYOgZZV1d3CqYXfvd49u\SCob4LtZZRqd25? MlXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyNkK5PVMoRjJzME[yPVk{RC:jPh?=
PC-9  NEHNd5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYG4SFBLOC1|IN88US=> NInaN4M4OiCq M2PXZ2lEPTB;MD64JO69VQ>? M1zFWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkKwOFc1Lz5{MUKyNFQ4PDxxYU6=
HCC827 NFXJSHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVGwMVMh|ryP MX63NkBp Mki4TWM2OD1yLkOg{txO M3\xTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkKwOFc1Lz5{MUKyNFQ4PDxxYU6=
HCC1937 M2DLfWZ2dmO2aX;uJGF{e2G7 M2H6UlEh|ryP MX:yOEBp NGK5c3Vz\WS3Y3XzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiQVvU NVr4c2RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFg2QTBpPkKyOFg5PTlyPD;hQi=>
SUM1315MO2 MoPMSpVv[3Srb36gRZN{[Xl? NETuUpMyKM7:TR?= NFvkc5IzPCCq NEXyc2tz\WS3Y3XzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiQVvU NFe2VY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4PFU6OCd-MkK0PFg2QTB:L3G+
SUM149PT MkX6SpVv[3Srb36gRZN{[Xl? MX2xJO69VQ>? M3\VcVI1KGh? Ml3CdoVlfWOnczD0bIUheGixc4Doc5J6dGG2aX;uJI9nKEGNVB?= NH\WOZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4PFU6OCd-MkK0PFg2QTB:L3G+
MDA-MB-436 MVTGeY5kfGmxbjDBd5NigQ>? MlnVNUDPxE1? M4jCdFI1KGh? MnjndoVlfWOnczD0bIUheGixc4Doc5J6dGG2aX;uJI9nKEGNVB?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6OEW5NEc,OjJ2OEi1PVA9N2F-
KMS12-BM  M3LLWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYKwMVIh|ryP MWiyOEBp Ml;4TWM2OO,:nkKg{txO NU\WdZNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlkzOzRpPkKyPFI6OjN2PD;hQi=>
NCI-H929 NIq0foVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXiwMVIh|ryP MlXNNlQhcA>? NVHtRY5DUUN3ME2wMlI2KM7:TR?= Mlf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MkmyN|QoRjJ{OEK5NlM1RC:jPh?=
MM1S NX3lNHZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHvNE0zKM7:TR?= M2TmXVI1KGh? MXTJR|UxRTBwNTFOwG0> NVvOeXNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlkzOzRpPkKyPFI6OjN2PD;hQi=>
MOLT-16 M4PBUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LjVVHjiIoQvF2= M4LLSVch\A>? NYLxbWxT\GWlcnXhd4V{KHSqZTDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 MmWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyM{iyO|MoRjJ|MEO4Nlc{RC:jPh?=
LOUCY MkT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYmx5qCK|ryP NVHJXXFxPyCm NHvISm1l\WO{ZXHz[ZMhfGinIHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? M{n4[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEO4Nlc{Lz5{M{CzPFI4OzxxYU6=
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MOLT-4 MlH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLGRY4y6oDLzszN M4K1ZVch\A>? M{fRcoRm[3KnYYPld{B1cGViY3XscEBvfW2kZYKgd4lodmmoaXPhcpRtgQ>? MljVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyM{iyO|MoRjJ|MEO4Nlc{RC:jPh?=
Jurkat MkThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUi2cVNpOeLCid88US=> NF\CSWM4KGR? Mn7p[IVkemWjc3XzJJRp\SClZXzsJI52dWKncjDzbYdvcW[rY3HueIx6 NH;tPG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CzPFI4Oyd-MkOwN|gzPzN:L3G+
RPMI-8402 Mlz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXKx5qCK|ryP M4LURVch\A>? NIjrWmxl\WO{ZXHz[ZMhfGinIHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB|OEK3N{c,OjNyM{iyO|M9N2F-
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Assay
Methods Test Index PMID
Western blot p-AKT / AKT / p-S6K / S6K / p-S6 / S6 / p-p38 / p38 ; p-GSK3 / GSK3 / p-BAD / BAD / p-MDM2 / MDM2 / p-p27 / p27 ; β-TrCP1 / p-mTOR 18922908 25721419
Immunofluorescence autophagosomes / autolysosomes 26814436
Growth inhibition assay Cell viability 25721419
In vivo When tumors reach 50-100 mm3, animals are randomized and treated with vehicle or PI-103. PI-103 exhibits significant activity, decreasing average tumor size by 4-fold after 18 days. [2] Mice treated with PI-103 have no obvious signs of toxicity premorbidly (based on body weight, food and water intake, activity, and general exam) or at necropsy. Treated tumors display decreased levels of phosphorylated Akt and S6, consistent with blockade of p110α and mTOR. PI-103 treatment is cytostatic to glioma xenografts. [2]

Protocol (from reference)

Kinase Assay:[3]
  • Enzyme Assays:

    Phosphatidylinositide 3-kinase inhibitory activity was determined using a scintillation proximity assay in the presence of 1 μmol/L ATP. Inhibition of mTOR protein kinase was determined using a TR-FRET-based LanthaScreen method from Invitrogen. Compounds were assayed at a maximum concentration of 10 μmol/L in the presence of 1 μmol/L ATP, and IC50 values were determined using GraphPad Prism software.

Cell Research:[2]
  • Cell lines: U87MG cells
  • Concentrations: 0.5 μM
  • Incubation Time: 24 hours
  • Method: U87MG cells are treated with PI-103 for 24 hours. Cell death is quantified by colorimetric determination of LDH activity using a cytotoxicity detection kit. Percentage of cell death (mean of three 12-well plates per experimental point) is calculated [(experimental value- low control)/(high control -low control) × 100], where the low-control cells are DMSO treated and high-control cells are Triton treated (1% Triton X-100, 30 min, 37 °
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: 6- to 12-week-old Balbc nu/nu mice bearing U87MG:ΔEGFR cells
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 24 mg/mL
(68.89 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
For best results, use promptly after mixing.

1 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 348.36
Formula

C19H16N4O3

CAS No. 371935-74-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1COCCN1C2=NC(=NC3=C2OC4=C3C=CC=N4)C5=CC(=CC=C5)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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