Wortmannin

Catalog No.S2758 Synonyms: KY 12420

Wortmannin Chemical Structure

Molecular Weight(MW): 428.43

Wortmannin is the first described PI3K inhibitor with IC50 of 3 nM in a cell-free assay, with little selectivity within the PI3K family. Also blocks autophagosome formation and potently inhibits DNA-PK/ATM with IC50 of 16 nM and 150 nM in cell-free assays.

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  • L3.6pl cells at 6,000 cells per well were incubated in MEM with 5% FBS in triplicate in a 96-well culture plate and then treated alone with 5 umol/L BMS-777607, 10 umol/L wortmannin, or with BMS-777607 in combination with individual inhibitors. Polyploidy was examined under BK71 Olympus microscope and photographed 72 hours after treatment.

    Mol Cancer Ther 2014 13(1), 37-48. Wortmannin purchased from Selleck.

    The effects of KITL on the differentiation of stem Leydig cells. Seminiferous tubules were cultured in the presence of various concentrations of KITL (K) without (C) or with PI3K inhibitor wortmannin (W) for 21 days. Panel A, KITL (0-100 ng/ml); Panel B, KITL (K) alone or with W (100 nM), K1 = KITL 1 ng/ml, K100 = 100 ng/ml. Medium testosterone (T) levels were measured. Mean ± SEM, n = 4-8. Identical letters indicate no significant difference between two groups at P < 0.05. W:Wortmannin

    Mol Cell Endocrinol, 2017, 444:1-8. Wortmannin purchased from Selleck.

  • Int J Mol Sci 2013 14(9), 17304-18. Wortmannin purchased from Selleck.

    Int J Mol Sci 2013 14(9), 17304-18. Wortmannin purchased from Selleck.

  • Cell growth inhibition of non-small cell lung carcinoma (NSCLC) by inhibitor of phosphoinositide 3-kinases (PI3Ks) Wortmannin. NCI-H460 and its multi-drug resistant (MDR) counterpart NCI-H460/R were subjected to Wortmannin. According to the results obtained, the effect of Wortmannin is dependent on the existence of MDR in the range of tested concentrations.

    2014 Dr.Milica Pesic from Institute for Biological Research. Wortmannin purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description Wortmannin is the first described PI3K inhibitor with IC50 of 3 nM in a cell-free assay, with little selectivity within the PI3K family. Also blocks autophagosome formation and potently inhibits DNA-PK/ATM with IC50 of 16 nM and 150 nM in cell-free assays.
Targets
PI3K [3]
(Cell-free assay)
DNA-PK [12]
(Cell-free assay)
ATM [12]
(Cell-free assay)
MLCK [1]
(Cell-free assay)
3 nM 16 nM 150 nM 170 nM
In vitro

The inhibition of MLCK by Wortmannin is not affected by calmodulin or peptide substrat, while reduced by high concentration of ATP. Wortmannin directly interacts with the catalytic domain of MLCK and leads to an irreversible loss of the enzyme activity. Wortmannin has no inhibitory to cAMP-dependent protein kinase, cGMP-dependent protein kinase, and calmodulin-dependent protein kinase II, and has little effect on protein kinase C activity. [1] Wortmannin inhibits N-formylmethionyl-leucylphenylalanine (fMLP)-stimulated PtdInsP3 (phosphatidylinositol 3,4,5-trisphosphate) formation with IC50 of 5 nM and this inhibition is completely abolished when pretreated with 100 nM Wortmannin in human neutrophils, with increased PtdInsP2 levels and no effects on cellular PtdInsP and PtdIns contents. Wortmannin could develop oscillatory changes in F-actin content and does not inhibit fMLP-stimulated actin polymerization in neutrophils. [2] Wortmannin irreversibly inhibits phosphatidylinositol 3-kinase (PI3-kinase) activity with binding to the 110-kDa protein (IC50 of 3 nM) and has no effect PI4-kinase in RBL-2H3 cells. Wortmannin also inhibits both Fc epsilon RI-mediated histamine secretion and leukotriene release, with no effect on the activation of the tyrosine kinase Lyn. [3] Wortmannin completely abolishes the insulin-induced hexose uptake in isolated rat adipocytes at 0.1 μM, without impairing isoproterenol-stimulated lipolytic activity. [4] Wortmannin suppresses insulin-induced production of nitric oxide by 50% at 500 nM in human umbilical vein endothelial cells, which is in response to IGF-1. [5] Wortmannin suppresses DNA double strand break (DSB) repair and has no effect on DSB levels or the kinetics of single strand break (SSB) repair in Chinese hamster ovary cells at 50 μM. Wortmannin could potentiate ionizing radiation (IR)-induced cytotoxicity with no toxicity by itself. [6] Wortmannin inhibits polo-like kinase (PLK1) activity IC50 of 24 nM in intact G2/M-arrested cells. [7] Wortmannin increases Toll-like receptor (TLR)-mediated accumulation of IL-6 in human macrophages with EC50 of 50 nM. Meanwhile Wortmannin significantly enhances TLR-mediated inducible nitric-oxide synthase (iNOS) expression and nitrite accumulation in mouse macrphages. Wortmannin activates the nuclear factor-κB and up-regulates the cytokine mRNA production. [8] Wortmannin also inhibits Polo-like kinase (PlK) 1 and PlK3, which play important roles in mitosis. Wortmannin treatment could lead to a reduction in phosphorylation of p53 on serine 20 induced by DNA damage. [9] Wortmannin suppresses hyaluronan-induced Akt phosphorylation and cell motility/migration in SW1990 cells. [10]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A459 NIPxOZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLGO3YzNjVizszN MnTvNU01KGR? NUHxVnNITE2VTx?= MYnlcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKHS{ZXH0cYVvfCC5aYToJJRidW:6aX\lci=> MVeyOVQ6ODN6Mx?=
H1703 M4\jfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV:yMlUh|ryP NIW4W3cyNTRiZB?= M2rh[mROW09? MnXU[Y5p[W6lZYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiC2cnXheI1mdnRid3n0bEB1[W2xeHnm[Y4> MXmyOVQ6ODN6Mx?=
HUVECs MnztR5l1d3SxeHnjbZR6KEG|c3H5 MVWxNFAhdk1? NG\Jb3czPCCq MlPIZZR1\W63YYTld{B1cGViYXLyc4difGm4ZTDl[oZm[3S|IH;mJINidHmlb4PpckBwdiCYUlmtbY5lfWOnZDDjfZRwfG:6aXPpeJk> NF;E[I0zPTR3MEG4Oi=>
APRE-19 M1;lSGFxd3C2b4Ppd{BCe3OjeR?= Mn7UOUDPxE1? MW[yOEBp NEjuT5hi[m:uaYPo[ZMhTkycLX3l[IlifGWmIIDyc{1{fXK4aY\hcE9idnSrLXHwc5B1d3OrczDhZ5Rqfmm2eR?= NIfWeVQzPTN{OU[xOy=>
MDA-MB-231 MUDBdI9xfG:|aYOgRZN{[Xl? MmTJNeKh|ryPwrC= NYX0c3VsPDhiaB?= MlvySG1UVw>? MUfk[YNz\WG|ZYOgeIhmKGOnbHygd5Vzfmm4YXygeJJm[XSnZDD3bZRpKDJ3IN88UUBw\iCIMTDvdkBHOiEEoB?= M{G0TVI2OzByOUOy
MCF7 NGnp[4pHfW6ldHnvckBCe3OjeR?= M4roVFExOCCwTR?= Mm\LNlQhcA>? MlnC[YxqdWmwYYTld{BGOi2rbnT1Z4VlKEGURT3MeYMh[WO2aY\peJk> NYO2PVI1OjVzN{K1OVc>
HT-29  M4fxSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT1bXNrOS53wrFCuW0> MkHjPVYhcA>? M4HlVIRm[3KnYYPld{Bk\WyuIHfyc5d1cCC5aHnjbEBk[W5iYnWgbY5pcWKrdHXkJIJ6KEu\TlG= MXuyOVAyOjF{Mx?=
MO59K  MoTRR5l1d3SxeHnjbZR6KEG|c3H5 NXzrWWhuPcLizszN MmG0O{Bl Mo[5SG1UVw>? NEXNZnZmdmijbnPld{B1cGViY4n0c5RwgGmlaYT5JI9nKGW2b4Dvd4ll\SCxcjDjbZNxdGG2aX6= MVmyOFk2OzV4MR?=
MO59J MYfDfZRwfG:6aXPpeJkhSXO|YYm= NEDaOFI2yqEQvF2= MlrFO{Bl M{P1fWROW09? NXn4c25D\W6qYX7j[ZMhfGinIHP5eI91d3irY3n0fUBw\iCndH;wc5Nq\GVib4KgZ4l{eGyjdHnu NVezblFVOjR7NUO1OlE>
MO59K  NG\VbFBCeG:ydH;zbZMhSXO|YYm= M1TiUlExKM7:TR?= M3nId|I1KGh? NGPKSnFFVVOR NYjKWJJmcW6lcnXhd4V{KHSqZTDEV2IhdGW4ZXygbY5lfWOnZDDifUBmfG:yb4Pp[IUhd3JiY3nzdIxifGmw NVv6PHBROjR7NUO1OlE>
MO59J NW\3XmJNSXCxcITvd4l{KEG|c3H5 MnTyNVAh|ryP MlXaNlQhcA>? NETJT4hFVVOR MUnpcoNz\WG|ZYOgeIhmKESVQjDs[ZZmdCCrbnT1Z4VlKGK7IHX0c5Bwe2mmZTDvdkBkcXOybHH0bY4> MWCyOFk2OzV4MR?=
HepG2 MXHGeY5kfGmxbjDBd5NigQ>? NUnDXGljOTByIH7N M4mzR|AvPSCq Ml;kSG1UVw>? NGKy[21jdG:la4OgUWEucW6mdXPl[EBCc3RicHjvd5Bpd3K7bHH0bY9v MVyyOFg3OzN3MB?=
A549  MkTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\ON{DDvU4EoB?= Mln5NkBp NFnvNHB{fXCycnXzd4V{KHCnbXX0doV5\WRvaX7keYNm\CCDa4SgZY5lKEeVS{ROtkBi[3SrdnH0bY9vNCCVLYDoZZNmKGG{cnXzeEwh[2WubDDhdI9xfG:|aYOgZY5lKGOjc4Dhd4UuOyCjY4TpeoF1cW:w NHPZNWozPDh2N{i2Ny=>
A549  MnzaSpVv[3Srb36gRZN{[Xl? M1\tbFExyqEQvH5CpC=> NVfVemd4OTZiaB?= NHTlT3RFVVOR M2HIbY1w\HWuYYTld{B1cGViSVHWJJJmeGyrY3H0bY9vKGGwZDDjZZV{\XNicnX0[Y51cW:wIH;mJG5RKGmwIITo[UBvfWOuZYXzMi=> MYKyOFgxOjFzMR?=
SK-N-LO MXfGeY5kfGmxbjDBd5NigQ>? MVqxNFAhdk1? M1;rdFAvPSCq M1i1OYRm[3KnYYPld{B1cGVic4TpcZVt[W62IHXm[oVkfHNib3[gcY9zeGirbnWgc44hSWu2IIDoc5NxcG:{eXzheIlwdg>? NYnjZ2N2OjR4NUS2NFY>
HL-60 NVKzfVZ3TnWwY4Tpc44hSXO|YYm= MUKwMlHDqM7:TR?= MWW3NkBp NG\YUJBjdG:la4Og[IF{[XSrbnniMYlv\HWlZXSgZ4VtdCCmaX\m[ZJmdnSrYYTpc44> M3n0NVI1PjB5Mkez
HepG2  MYnGeY5kfGmxbjDBd5NigQ>? MmXaNlAxKG6P NWXVWIdsOC53IHi= MljIZZR1\W63YYTld{BHd3iRIIDoc5NxcG:{eXzheIlwdg>? MWqyOFU{PTF7Mh?=
H520 M{H2XGZ2dmO2aX;uJGF{e2G7 MWCxNOKh|ryP M{\4NlEhcA>? MVnEUXNQ MXnk[YNz\WG|ZYOgZ4VtdHWuYYKgdIhwe3Cqbz3BT3QheHKxdHXpckBt\X[nbIO= NYH0XJpzOjR2NEe5N|U>
H1975 MV\GeY5kfGmxbjDBd5NigQ>? NHPMdpoyOMLizszN MnzENUBp NFTINGtFVVOR NXrUOoQ4\GWlcnXhd4V{KGOnbHz1cIFzKHCqb4PwbI8uSUuWIIDyc5RmcW5ibHX2[Yx{ NV;5WZh5OjR2NEe5N|U>
MG-63 M{nvOWFxd3C2b4Ppd{BCe3OjeR?= NVn3SW9sOTBiwsXN NWPkbXlyOTJiaB?= MlHY[Y5p[W6lZYOgSHAucW6mdXPl[EBieG:ydH;zbZM> MofQNlQ{PTh|MEG=
5637 NYjJOJZvSXCxcITvd4l{KEG|c3H5 MkDzNVDDqM7:TR?= NYXaeWdmPDBibXnu NYnYcHBYemW4ZYLz[ZMheDJzV1HGNUBmgHC{ZYPzbY9vNCCFRFug[ZhxemW|c3nvckwh[W6mIHPlcIwhcW6qaXLpeIlwdiCrbnT1Z4VlKGK7IH\1Z49q\GGw NYHXd|Q2OjR|M{O4Olg>
HEK-293 MnPkSpVv[3Srb36gRZN{[Xl? NHzXR3cyPTCwTR?= MYOxOkBp M3XQO2ROW09? NX\NWHNs\GWlcnXhd4V{KEOUVDDhZ5Rqfmm2eR?= NGq3eIszPDN{NEO2Oi=>
SW480  MnSwSpVv[3Srb36gRZN{[Xl? MmnXNVUxdk1? M4C5R|IxKGh? NH2wZ3ZFVVOR M{PufpJm\HWlZYOgZ4VtdHWuYYKgZYNkfW23bHH0bY9vKG:oIN8yMYNifGWwaX6= NFPtU3EzPDN{NEO2Oi=>
HepG2 NHPFZnpHfW6ldHnvckBCe3OjeR?= NYjJUmZ7OTByIH7N NUTHR4pOOjRiaB?= M1rDUoF1fGWwdXH0[ZMhfGinIHPvcI9vcWW|IH;mJJRp\SC2dX3vdkBk\WyuczD3bZRpKHWycnXneYxifGmxbjDv[kBCc3Rz MlPYNlQzQTd3MUC=
HCT 116  MUnGeY5kfGmxbjDBd5NigQ>? MV[xNFAhdk1? M33KVFI1KGh? NGnMVlBifHSnboXheIV{KHSqZTDjc4xwdmmnczDv[kB1cGVidIXtc5Ih[2WubIOge4l1cCC3cILl[5Vt[XSrb36gc4YhSWu2MR?= MX[yOFI6PzVzMB?=
BEL/FU MkjtSpVv[3Srb36gRZN{[Xl? M1fudVEhdU1? NFX2NWkzPCCq M4\VcoRm[3KnYYPld{Bxem:2ZXnuJIxmfmWuczDv[kB1cGViUFmzT{9Cc3RicHH0bJdigQ>? NI[0TXYzPDJ|MkC5PS=>
Huh7  NXeyR4R[TnWwY4Tpc44hSXO|YYm= MkHoN:Kh|ryP NWrEOpFXOSCq M3LUbpJm\HWlZYOgeIhmKH[rcoXzJIVvfHK7IHnueI8hfGinIHPlcIx{ NFW4Sm0zPDF6NEG5Oi=>
A-375 MkW0RZBweHSxc3nzJGF{e2G7 Mn\mOE85KM7:TR?= NGn3UIkzPCCq NWfRPGUy\W6qYX7j[ZMhXFKDSVytbY5lfWOnZDDhdI9xfG:|aYRCpC=> NEXJXGQzPDFzM{G3Ny=>
A-375-TS  NYHKd|U5SXCxcITvd4l{KEG|c3H5 NF\lZZc1NzhizszN M{Ow[|I1KGh? MoPN[Y5p[W6lZYOgWHJCUUxvaX7keYNm\CCjcH;weI9{cXQEoB?= M2jkPFI1OTF|MUez
Mel-HO NVzrXVVuSXCxcITvd4l{KEG|c3H5 M3ntZlQwQCEQvF2= M4LYNVI1KGh? NHjRPItmdmijbnPld{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orc9Mg NFO5NZMzPDFzM{G3Ny=>
Mel-HO-TS NYH3XHRXSXCxcITvd4l{KEG|c3H5 MkPDOE85KM7:TR?= M{DUVVI1KGh? NHriXW5mdmijbnPld{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orc9Mg MWeyOFEyOzF5Mx?=
MeWo NHf6UlFCeG:ydH;zbZMhSXO|YYm= Mn;nOE85KM7:TR?= Mn20NlQhcA>? NUe3WY86\W6qYX7j[ZMhXFKDSVytbY5lfWOnZDDhdI9xfG:|aYRCpC=> NYLwWWVpOjRzMUOxO|M>
Mel-2a M1PnSGFxd3C2b4Ppd{BCe3OjeR?= NF3BVXo1NzhizszN NHHUS2gzPCCq NE\4PYVmdmijbnPld{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orc9Mg MYiyOFEyOzF5Mx?=
MDA-MB-231 NFLLRnJHfW6ldHnvckBCe3OjeR?= MofyNQKBmzRyMDDuUS=> MVG0JIg> MX3zeZBxemW|c3XzJGFsfCCyaH;zdIhwenmuYYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M4O5TFIzQTB4MkW5
MDA-MB-231 NUHpNWRNTnWwY4Tpc44hSXO|YYm= NELpUXQ1ODBibl2= M1m4[FQhcA>? MkS1[IVkemWjc3XzJG1OWC17IHHu[EBKVC16IIDyc5RmcW5iaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3nXXVIzQTB4MkW5
Jurkat NIDQTnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV:wMlI2NTFwMkWg{txO MVqyOE81QCCq MWDEUXNQ MWjpcohq[mm2czDj[YxtKHC{b3zp[oVz[XSrb36gbY4h[m:2aDD0bY1mNSCjbnSg[I9{\S1iZHXw[Y5l\W62IH3hco5meg>? MUixPVc2PzF6NR?=
Namalwa M3\YcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfxd|M4OC5{NT2xMlI2KM7:TR?= MmTPNlQwPDhiaB?= Mni1SG1UVw>? Mn3rbY5pcWKrdIOgZ4VtdCCycn;sbYZmemG2aX;uJIlvKGKxdHigeIlu\S1iYX7kJIRwe2VvIHTldIVv\GWwdDDtZY5v\XJ? M3HCS|E6PzV5MUi1
Jurkat M3P5NGFxd3C2b4Ppd{BCe3OjeR?= NVrXb2pTOC5{NT2xMlI2KM7:TR?= MWiyOE81QCCq MVnEUXNQ M2rj[4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZo91cCC2aX3lMUBidmRiZH;z[U0h\GWyZX7k[Y51KG2jbn7ldi=> M{jtflE6PzV5MUi1
Namalwa NFuwZW9CeG:ydH;zbZMhSXO|YYm= NFSxWYwxNjJ3LUGuNlUh|ryP M4HLZlI1NzR6IHi= MVPEUXNQ M{DWXYlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZo91cCC2aX3lMUBidmRiZH;z[U0h\GWyZX7k[Y51KG2jbn7ldi=> NH2xUGgyQTd3N{G4OS=>
K562 NXrnSlhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\3R3pmOjRiaB?= M3zWO2lEPTB;MkZCtVAvOTRibl2= NEjB[WUyQTZ4MkO2NS=>
SW1990 M2TCNGZ2dmO2aX;uJGF{e2G7 NYrpV3VpOC5yMT2xJO69VQ>? M1;tSlEhcA>? MUTpcohq[mm2czDIRU1qdmS3Y3XkJGFsfCCyaH;zdIhwenmuYYTpc44> NWrwT5pEOTl2NkmwNlA>
RT112  MknpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXWxNOKh|ryP NH3WUoEzPCCq MV\EUXNQ MXTk[YNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[gS|IwVSClZXzsdy=> Mm\SNVg4QDd6M{K=
MHG-U1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVKxNOKh|ryP MYWyOEBp Mlr6SG1UVw>? NGTKd3ll\WO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4YhTzJxTTDj[Yxtew>? MX[xPFc5Pzh|Mh?=
SMMC-7721 NWjaSG5RSXCxcITvd4l{KEG|c3H5 Ml\ZNlAxyqCwTR?= M3LpcVI1KGh? NFT6e|BqdmO{ZXHz[ZMhS0i[LXnu[JVk\WRiYYDvdJRwe2m| MkPHNVc2PTdzOUG=
SMMC-7721 M3XXd2Z2dmO2aX;uJGF{e2G7 M2XkV|IxOMLibl2= M2rsOFI1KGh? NH;ab3B2eC2{ZXf1cIF1\XNizsKxMFRIXDFiZYjwdoV{e2mxbh?= M3KwNFE4PTV5MUmx
HeLa MUfGeY5kfGmxbjDBd5NigQ>? MVqxNFDDqG6PwrC= NUniXVdsOSCq Mk\lZYx1\XK|IITo[UBud3KyaH;sc4d6KG:oIITo[UB1emGwc3\ldpJqdiC{ZXP5Z4xqdmdiY3;tdIFzfG2nboS= MUexOlg6ODlzNR?=
MRC5VI Mn7QSpVv[3Srb36gRZN{[Xl? MmD4NVIvPSCvTR?= MoO3NE42KGh? Mon2SG1UVw>? M1PwWIFjd2yrc3jld{B1cGViU3XyOFc{N1SqckOwPOKheGixc4Doc5J6dGG2aX;uJI9nKEGtdGDLRuKh NYDFXphZOTZ{MkezPVQ>
AT5BIVA NVXhN5JuTnWwY4Tpc44hSXO|YYm= NIH3TWYyOi53IH3N NWnpVG1YOC53IHi= MWnEUXNQ MWHhZo9tcXOqZYOgeIhmKFOnckS3N{9VcHJ|MElCpJBpd3OyaH;yfYxifGmxbjDv[kBCc3SSS1NCpC=> NH3I[YMyPjJ{N{O5OC=>
M059J NH63TVBHfW6ldHnvckBCe3OjeR?= NEDKUGEyOi53IH3N MVmwMlUhcA>? MVLEUXNQ NXHtTHF3[WKxbHnzbIV{KHSqZTDT[ZI1PzNxVHjyN|A5yqCyaH;zdIhwenmuYYTpc44hd2ZiQXv0VGtDyqB? NHnubYYyPjJ{N{O5OC=>
HeLa MVPGeY5kfGmxbjDBd5NigQ>? NHLRXpYyOi53IH3N M2\m[lAvPSCq NVfGTWtnTE2VTx?= MXfhZo9tcXOqZYOgeIhmKFOnckS3N{9VcHJ|MElCpJBpd3OyaH;yfYxifGmxbjDv[kBCc3SSS1NCpC=> MlnsNVYzOjd|OUS=
N2a MYHBdI9xfG:|aYOgRZN{[Xl? NXixRpA4OC5zLUGwJO69VQ>? MXiyJIg> NHKyXoNqdmS3Y3XzJIRm[3KnYYPl[EBk\WyuII\pZYJqdGm2eTDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nch?= NE\KNm0yPTh2Mke2Oy=>
Jurkat  NWXjNGI1U2mwYYPlJGF{e2G7 M1;NOmlEPTBib3[gNlQhdk1? MUSxOVY3PDVzOR?=

... Click to View More Cell Line Experimental Data

In vivo Wortmannin inhibits peritoneal metastasis of SW1990 in mice at 1 mg/kg, without any weight loss. [10] Wortmannin inhibits phosphatidylinositide 3-kinase-protein kinase B (PKB)/Akt phosphorylation in both normal tissues (lung, heart and brain homogenates) and tumor tissue in mice, without mortality or acute toxicity at 0.7 mg/kg. Combination with LY188011, Wortmannin significantly increases apoptosis and inhibit tumor growth in orthotopic tumor, while both monotherapy could not. [11]

Protocol

Animal Research:[11]
+ Expand
  • Animal Models: Human pancreatic adenocarcinoma cells PK1 are injected both s.c. and orthotopically into SCID mice.
  • Formulation: Dissolved at 0.4 mg/mL in DMSO, and diluted with 0.9% NaCl before use
  • Dosages: 0.175, 0.35, and 0.7 mg/kg
  • Administration: Injected by i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 85 mg/mL (198.39 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 428.43
Formula

C23H24O8

CAS No. 19545-26-7
Storage powder
in solvent
Synonyms KY 12420

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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