AZD6482

For research use only.

Catalog No.S1462 Synonyms: KIN-193

19 publications

AZD6482 Chemical Structure

CAS No. 1173900-33-8

AZD6482 (KIN-193) is a PI3Kβ inhibitor with IC50 of 10 nM, 8-, 87- and 109-fold more selective to PI3Kβ than PI3Kδ, PI3Kα and PI3Kγ in cell-free assays. Phase 1.

Size Price Stock Quantity  
EUR 167 In stock
EUR 314 In stock
EUR 953 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's AZD6482 has been cited by 19 publications

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description AZD6482 (KIN-193) is a PI3Kβ inhibitor with IC50 of 10 nM, 8-, 87- and 109-fold more selective to PI3Kβ than PI3Kδ, PI3Kα and PI3Kγ in cell-free assays. Phase 1.
Targets
PI3Kβ [1]
(Cell-free assay)
PI3Kδ [1]
(Cell-free assay)
DNA-PK [1]
(Cell-free assay)
PI3Kα [1]
(Cell-free assay)
10 nM 80 nM 420 nM 870 nM
In vitro

AZD6482 also inhibits PI3Kα, γ, and δ, with IC50 of 80 nM to 1.09 μM, which are significantly lower than its (+)-enantiomer (S-form). AZD6482 is an antiplatelet agent; it blocks platelet activation adhesion/aggregation and promotes platelet disaggregation in assay of washed platelet aggregation (WPA), with an IC50 value of 6 nM. Furthermore, by targeting PI3Kβ, AZD6482 specifically inhibits thrombosis without interfering with normal haemostasis. Therefore, AZD6482 is used as an anti-thrombotic drug for the prophylaxis of thrombotic disorders. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human RXF393 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKGi3bXHuJHJZTjN7MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNFEyPTRizszN MVPTRW5ITVJ?
human SW982 cell NVjJU5RuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUfJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6wN|U5PCEQvF2= M4L5e3NCVkeHUh?=
human MAD-MB-468 cells MoriSpVv[3Srb36gZZN{[Xl? NVrVNmdUUW6qaXLpeIlwdiCxZjDQTVNM[mW2YTDpckBpfW2jbjDNRWQuVUJvNE[4JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiU3XyOFc{KEGtdDDwbI9{eGixconsZZRqd25iYomgZ4VtdHWuYYKgdI91\W6leTDhd5NigSxiSVO1NF0xNjB2IN88US=> M4W1c|I1QTl{OEe0
human KURAMOCHI cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NELIWmpKdmirYnn0bY9vKG:oIHj1cYFvKEuXUlHNU2NJUSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUC1NUDPxE1? M33NUHNCVkeHUh?=
human A498 cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHS0ZpJKdmirYnn0bY9vKG:oIHj1cYFvKEF2OUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlEyPzl3IN88US=> MX3TRW5ITVJ?
human YT cell MoX1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NY\FfI9HUW6qaXLpeIlwdiCxZjDoeY1idiC\VDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVIxPjZizszN NIe4[2hUSU6JRWK=
human VMRC-RCZ cell MlP3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF\seIVKdmirYnn0bY9vKG:oIHj1cYFvKF[PUlOtVmNbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNEi5PEDPxE1? Ml61V2FPT0WU
human MDA-MB-415 cell NF7qUlVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NG\RVpVKdmirYnn0bY9vKG:oIHj1cYFvKE2GQT3NRk01OTViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG2O|MzKM7:TR?= NEflTZJUSU6JRWK=
human J82 cell MkHES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGo5OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNEe3O|kh|ryP M3XGfXNCVkeHUh?=
human SW1710 cell M2TQNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2PGZWlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42Ojl{IN88US=> NHPCXmNUSU6JRWK=
human T47D cell M4T5eWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkWzNlch|ryP M33LWXNCVkeHUh?=
human SU-DHL-1 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV6x[VNkUW6qaXLpeIlwdiCxZjDoeY1idiCVVT3ETGwuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNU[4NVMh|ryP NF3PSlhUSU6JRWK=
human BT-20 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnX5TY5pcWKrdHnvckBw\iCqdX3hckBDXC1{MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOVkzOjJizszN MoPiV2FPT0WU
human OS-RC-2 cell NFnxfphIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF3WW4RKdmirYnn0bY9vKG:oIHj1cYFvKE:VLWLDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ{M{i4JO69VQ>? NFnvTpVUSU6JRWK=
human RPMI-6666 cell M1PZT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIO3PYFKdmirYnn0bY9vKG:oIHj1cYFvKFKSTVmtOlY3PiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNkSzO|kh|ryP M3fpcHNCVkeHUh?=
human OVCAR-3 cell MonwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI\aW2ZKdmirYnn0bY9vKG:oIHj1cYFvKE:YQ1HSMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ5OUS2JO69VQ>? NGXQRoNUSU6JRWK=
human NALM-6 cell MnPXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1vIZWlvcGmkaYTpc44hd2ZiaIXtZY4hVkGOTT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44ODR4NTFOwG0> NUDReFQ1W0GQR1XS
human RS4-11 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\qTY5pcWKrdHnvckBw\iCqdX3hckBTWzRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlc{QDB4IN88US=> MXPTRW5ITVJ?
human SK-MES-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHnPPZRKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FV{0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55OEm2O{DPxE1? Mo\sV2FPT0WU
human ES7 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEPqSplKdmirYnn0bY9vKG:oIHj1cYFvKEWVNzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFEzQDZizszN NIDyU|lUSU6JRWK=
human NCI-H2342 cell NVfI[WR[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmDLTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKzOFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh2Mkm0JO69VQ>? MXrTRW5ITVJ?
human SCC-9 cell M4rLPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1LMc2lvcGmkaYTpc44hd2ZiaIXtZY4hW0OFLUmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg2PzdizszN MUPTRW5ITVJ?
human EW-7 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml2zTY5pcWKrdHnvckBw\iCqdX3hckBGXy15IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64OlM2PiEQvF2= NF\TVW9USU6JRWK=
human HH cell MnfjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{eyXGlvcGmkaYTpc44hd2ZiaIXtZY4hUEhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkm2NlIzKM7:TR?= MY\TRW5ITVJ?
human A427 cell NIjZVHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWfadHVsUW6qaXLpeIlwdiCxZjDoeY1idiCDNEK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46QTVyNTFOwG0> MVjTRW5ITVJ?
human Daudi cell M2XWOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWjSWpNLUW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODZ6OEig{txO MUXTRW5ITVJ?
human P30-OHK cell NVv4[mNzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{jBN2lvcGmkaYTpc44hd2ZiaIXtZY4hWDNyLV;IT{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODdzM{ig{txO M332ZXNCVkeHUh?=
human HGC-27 cell M1S4bGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYq0fJd1UW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTFwMUixOkDPxE1? NGPCSXRUSU6JRWK=
human CAMA-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWXJcohq[mm2aX;uJI9nKGi3bXHuJGNCVUFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlAxOSEQvF2= NFX1XlZUSU6JRWK=
human SK-OV-3 cell MojRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlPBTY5pcWKrdHnvckBw\iCqdX3hckBUUy2RVj2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zODR2NDFOwG0> MXrTRW5ITVJ?
human NCI-H1048 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEL5UG1KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVA1QCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMkK1O|kh|ryP M3nFVHNCVkeHUh?=
human MEL-HO cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{nhZ2lvcGmkaYTpc44hd2ZiaIXtZY4hVUWOLVjPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zPDJ|ODFOwG0> M2LWbXNCVkeHUh?=
human NKM-1 cell M1n0NWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFT5cnJKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zQDZ|NjFOwG0> MkizV2FPT0WU
human NCI-H650 cel MonkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVrJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNkWwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODB5NjFOwG0> NFnjdGFUSU6JRWK=
human HAL-01 cell NG\GSlZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4iyOGlvcGmkaYTpc44hd2ZiaIXtZY4hUEGOLUCxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODF5NTFOwG0> MYPTRW5ITVJ?
human 786-0 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\MTY5pcWKrdHnvckBw\iCqdX3hckA4QDZvMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|EzQDNizszN NVe3c3VqW0GQR1XS
human GI-1 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmPUTY5pcWKrdHnvckBw\iCqdX3hckBIUS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zNVgxQCEQvF2= M3HERnNCVkeHUh?=
human Becker cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M33ENmlvcGmkaYTpc44hd2ZiaIXtZY4hSmWla3XyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjNzNTFOwG0> NGjBWIxUSU6JRWK=
human CCF-STTG1 cell MoPqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV3VV5B7UW6qaXLpeIlwdiCxZjDoeY1idiCFQ1[tV3RVTzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkSyOVI2KM7:TR?= MV7TRW5ITVJ?
human MC116 cell MkDSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX7p[2N3UW6qaXLpeIlwdiCxZjDoeY1idiCPQ{GxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDZzMkKg{txO MofuV2FPT0WU
human MDA-MB-468 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWHJcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj20Olgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjV|N{O2JO69VQ>? NF7KTFVUSU6JRWK=
human NB12 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlHxTY5pcWKrdHnvckBw\iCqdX3hckBPSjF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT61OFg1OiEQvF2= M2XqcXNCVkeHUh?=
human LXF-289 cell NXPrclBYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF35cFVKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjV3MUC0JO69VQ>? MlHDV2FPT0WU
human MFE-296 cell MoDGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4rFe2lvcGmkaYTpc44hd2ZiaIXtZY4hVU[HLUK5OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTZ6M{[g{txO Mlz2V2FPT0WU
human SNU-423 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFTORpRKdmirYnn0bY9vKG:oIHj1cYFvKFOQVT20NlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjV5MUmyJO69VQ>? MYnTRW5ITVJ?
human NCI-H2291 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXvVfWcyUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIzQTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkW3OlEh|ryP NXSw[GdDW0GQR1XS
human OCUB-M cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWPJcohq[mm2aX;uJI9nKGi3bXHuJG9EXUJvTTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVg{OTRizszN NVvmbGxvW0GQR1XS
human KU812 cell NWjZNI9jT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUfuXHFyUW6qaXLpeIlwdiCxZjDoeY1idiCNVUixNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTl4Mkig{txO NGXLfG1USU6JRWK=
human HuO9 cell NY\Y[3FGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3TNVmlvcGmkaYTpc44hd2ZiaIXtZY4hUHWROTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlEzQCEQvF2= M1[0TnNCVkeHUh?=
human 639-V cell NUn4[Vc5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUXJcohq[mm2aX;uJI9nKGi3bXHuJFY{QS2YIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|E1PCEQvF2= NGDhUXBUSU6JRWK=
human HCC70 cell M3\iZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJGhESzdyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62OlE1PiEQvF2= M3vxSXNCVkeHUh?=
human SNB75 cell M{fnOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn7wTY5pcWKrdHnvckBw\iCqdX3hckBUVkJ5NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlY2ODhizszN MYPTRW5ITVJ?
human D-336MG cell Mn7pS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVHJcohq[mm2aX;uJI9nKGi3bXHuJGQuOzN4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY3QDhizszN MW\TRW5ITVJ?
human LoVo cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknETY5pcWKrdHnvckBw\iCqdX3hckBNd1[xIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63N|gzPSEQvF2= NXvHSXV5W0GQR1XS
human EB2 cell NXHWWIRJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnTOTY5pcWKrdHnvckBw\iCqdX3hckBGSjJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLke1NFE6KM7:TR?= MnjHV2FPT0WU
human HSC-2 cell NHm4eVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWDJcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63OVczPyEQvF2= NXvm[lF7W0GQR1XS
human D-542MG cell NV;PWZRyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3K3XGlvcGmkaYTpc44hd2ZiaIXtZY4hTC13NELNS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDVyN{eg{txO Ml:5V2FPT0WU
human COLO-684 cell MlfnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIe3bG9KdmirYnn0bY9vKG:oIHj1cYFvKEORTF:tOlg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5{MECzNkDPxE1? NGr5d21USU6JRWK=
human GDM-1 cell NHHZZphIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXTJcohq[mm2aX;uJI9nKGi3bXHuJGdFVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6yOFIxPiEQvF2= NInoboxUSU6JRWK=
human SW1088 cell MlfES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYrJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTB6ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuOFgyPDNizszN MnfrV2FPT0WU
human SF295 cell NYXKeJRJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXrJcohq[mm2aX;uJI9nKGi3bXHuJHNHOjl3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj63Olk2PSEQvF2= NIDFPGNUSU6JRWK=
human NCI-H2030 cell M3;CNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3fyTGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFMxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi57MEK4PEDPxE1? NGPES4VUSU6JRWK=
human NCI-H1755 cell NF;TWHBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3m1dGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixO|U2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{N{C2N{DPxE1? M2mwTnNCVkeHUh?=
human MDA-MB-361 cell NVXqRYZ3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX3HXIM{UW6qaXLpeIlwdiCxZjDoeY1idiCPRFGtUWIuOzZzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz61NlcxQSEQvF2= NV7R[Zk{W0GQR1XS

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
- Collapse

Assay of PI3K enzyme inhibition:

The inhibition of PI3Kβ, PI3Kα, PI3Kγ, and PI3Kδ is evaluated in an AlphaScreen based enzyme activity assay using human recombinant enzymes. The assay measures PI3K-mediated conversion of PIP2 to PIP3. Biotinylated PIP3, a GST-tagged pleckstrin homology (PH) domain and the two AlphaScreen beads form a complex that elicits a signal upon laser excitation at 680 nm. The PIP3 formed in the enzyme reaction competes with the biotinylated PIP3 for binding to the PH domain thus reducing the signal with increasing enzyme product. The AZD6482 is dissolved in DMSO and added to 384 well plates. PBKβ, PBKα, PBKγ, or PBKδ is added in a Tris buffer (50 mM Tris pH 7.6, 0.05% CHAPS, 5 mM DTT, and 24 mM MgCl2) and allowed to preincubate with AZD6482 for 20 minutes prior to the addition of substrate solution containing PIP2 and ATP. The enzyme reaction is stopped after 20 minutes by addition of stop solution containing EDTA and biotin-PIP3, followed by addition of detection solution containing GST-grpl PH and AlphaScreen beads. Plates are left for a minimum of 5 hours in the dark prior to analysis. The final concentration of DMSO, ATP and PIP2 in the assay are, 0.8%, 4 μM, and 40 μM, respectively. IC50 values are calculated according to the equation, y = {a+[(b-a)/(l+(x/IC50)s)]}, where y = % inhibition; a = 0%; b = 100%; s = the slope of the concentration-response curve; x = AZD6482 concentration.
Cell Research:[1]
- Collapse
  • Cell lines: Human platelet pellet
  • Concentrations: 0–60 nM, dissolved in DMSO
  • Incubation Time: 5 min
  • Method: For assay of washed platelet aggregation (WPA), the platelet pellets are isolated from human blood and re-suspended to 2 × 1015/L in Tyrodes buffer (TB) containing 1 μM hirudin and 0.02 U/mL apyrase. Then, the platelet suspension is left to rest at room temperature for 30 min. Just prior to time for assay, CaCl2 is added to a final concentration of 2 mM. AZD6482, dissolved in DMSO, is added to a 96-well plate prior to the addition of the washed platelet suspension. The platelet suspension is preincubated with AZD6482 for 5 min. Light absorption at 650 nm is recorded before and after a 5 min plate shake and referred to as recording 0 (R0) and Rl. A mouse anti-human CD9 antibody is added (at a donor specific concentration) to each well prior to next 10 min plate shake and light absorption recording; R2. For data analysis, light absorbance in wells with TB are subtracted from all readings before percent aggregation is calculated according the formula: [(R1-R2)/R1] × 100 = % aggregation. Spontaneous aggregation or pro-aggregatory effect of the inhibitor is evaluated by the same formula, [(R0-Rl)/R0] × 100 = % aggregation. IC50 values are calculated according to the equation, y = {a+[(b-a)/(l+(x/IC50)s)]}, where y = % inhibition; a = 0%; b = 100%; s = the slope of the concentration-response curve; x = AZD6482 concentration.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 82 mg/mL (200.75 mM)
Ethanol 10 mg/mL (24.48 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 408.45
Formula

C22H24N4O4

CAS No. 1173900-33-8
Storage powder
in solvent
Synonyms KIN-193
Smiles CC1=CN2C(=O)C=C(N=C2C(=C1)C(C)NC3=CC=CC=C3C(=O)O)N4CCOCC4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

PI3K Signaling Pathway Map

PI3K Inhibitors with Unique Features

Related PI3K Products

Tags: buy AZD6482 | AZD6482 supplier | purchase AZD6482 | AZD6482 cost | AZD6482 manufacturer | order AZD6482 | AZD6482 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID