AZD6482

Catalog No.S1462 Synonyms: KIN-193

AZD6482 Chemical Structure

Molecular Weight(MW): 408.45

AZD6482 is a PI3Kβ inhibitor with IC50 of 10 nM, 8-, 87- and 109-fold more selective to PI3Kβ than PI3Kδ, PI3Kα and PI3Kγ in cell-free assays. Phase 1.

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Cited by 7 Publications

4 Customer Reviews

  • Cells treated with either control or INPP4B siRNA and following a 1-hour treatment with either DMSO or 1 mmol/L inhibitor; pPRAS40T246 and total PRAS40 were run on a separate blot for which the bottom Ku-86 panel is the loading control(pan-PI3Ki: GDC-0941, PI3K-ai: BYL719,PI3K-bi: AZD6482)

    Mol Cancer Res, 2017, 15(6):765-775. AZD6482 purchased from Selleck.

    Dose response curve of compound on melanoma cell lines.  Compound was dissolved in DMSO, added in a 5-fold dilution series, starting with 10 μM, and incubated for 72 hours.  Fluorescence was measured after 8 hours incubation in resazurin.  Data was normalized to DMSO (maximal viability) and Doxorubicin (minimum viability).

    One customer. AZD6482 purchased from Selleck.

  • After starved in serum-free medium for 24h,A549 cells incubated with the indicated concentrations of AZD6482 for 3h,followed by 20-minute stimolation of 100ng/ml EGF.

    Dr. Zhang of Tianjin Medical University. AZD6482 purchased from Selleck.

    Collagen-embedded melanoma spheroids were treated for 72 h with SGI-1776 [5 μM] or AZD6482 [10 μM] as single agents or in combination before staining for live (green) and dead (red) cells. Experiments were conducted in triplicate and representative images are shown. Scale bar represents 150 microns.

    Oncotarget, 2016, 7(34):54897-54912. AZD6482 purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description AZD6482 is a PI3Kβ inhibitor with IC50 of 10 nM, 8-, 87- and 109-fold more selective to PI3Kβ than PI3Kδ, PI3Kα and PI3Kγ in cell-free assays. Phase 1.
Targets
PI3Kβ [1]
(Cell-free assay)
PI3Kδ [1]
(Cell-free assay)
DNA-PK [1]
(Cell-free assay)
PI3Kα [1]
(Cell-free assay)
10 nM 80 nM 420 nM 870 nM
In vitro

AZD6482 also inhibits PI3Kα, γ, and δ, with IC50 of 80 nM to 1.09 μM, which are significantly lower than its (+)-enantiomer (S-form). AZD6482 is an antiplatelet agent; it blocks platelet activation adhesion/aggregation and promotes platelet disaggregation in assay of washed platelet aggregation (WPA), with an IC50 value of 6 nM. Furthermore, by targeting PI3Kβ, AZD6482 specifically inhibits thrombosis without interfering with normal haemostasis. Therefore, AZD6482 is used as an anti-thrombotic drug for the prophylaxis of thrombotic disorders. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human RXF393 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXzJcohq[mm2aX;uJI9nKGi3bXHuJHJZTjN7MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNFEyPTRizszN NVX0ZWFOW0GQR1XS
human SW982 cell M2TPOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1zkd2lvcGmkaYTpc44hd2ZiaIXtZY4hW1d7OEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlA{PTh2IN88US=> NHTCbIFUSU6JRWK=
human MAD-MB-468 cells Mo\TSpVv[3Srb36gZZN{[Xl? MmrtTY5pcWKrdHnvckBw\iCSSUPLZoV1[SCrbjDoeY1idiCPQVStUWIuPDZ6IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhW2W{NEezJGFsfCCyaH;zdIhwenmuYYTpc44h[nliY3XscJVt[XJicH;0[Y5kgSCjc4PhfUwhUUN3ME2wMlA1KM7:TR?= MV2yOFk6Ojh5NB?=
human KURAMOCHI cell M2LBU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MULJcohq[mm2aX;uJI9nKGi3bXHuJGtWWkGPT1PITUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTB3MTFOwG0> NFPSVllUSU6JRWK=
human A498 cell M2P4dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF\oXm9KdmirYnn0bY9vKG:oIHj1cYFvKEF2OUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlEyPzl3IN88US=> NGj4VnFUSU6JRWK=
human YT cell NEThTVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{nydWlvcGmkaYTpc44hd2ZiaIXtZY4hYVRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkGyNFY3KM7:TR?= MUDTRW5ITVJ?
human VMRC-RCZ cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXu5XVhZUW6qaXLpeIlwdiCxZjDoeY1idiCYTWLDMXJEYiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMUS4PVgh|ryP MWHTRW5ITVJ?
human MDA-MB-415 cell MlrLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVe3copuUW6qaXLpeIlwdiCxZjDoeY1idiCPRFGtUWIuPDF3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xOlc{OiEQvF2= MoC0V2FPT0WU
human J82 cell MmPvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUTXd5pMUW6qaXLpeIlwdiCxZjDoeY1idiCMOEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ4Pzd7IN88US=> M{LxNXNCVkeHUh?=
human SW1710 cell M1Xib2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3jNSWlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42Ojl{IN88US=> MY\TRW5ITVJ?
human T47D cell NYnGNnFvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NG\CU2RKdmirYnn0bY9vKG:oIHj1cYFvKFR2N1SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlU{OjdizszN M2XmVXNCVkeHUh?=
human SU-DHL-1 cell M1zVcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3y0ZmlvcGmkaYTpc44hd2ZiaIXtZY4hW1VvRFjMMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjV4OEGzJO69VQ>? NIjsdIpUSU6JRWK=
human BT-20 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHO3eZpKdmirYnn0bY9vKG:oIHj1cYFvKEKWLUKwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42QTJ{MjFOwG0> NX;TRpl2W0GQR1XS
human OS-RC-2 cell NVWwcplRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKGi3bXHuJG9UNVKFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlYzOzh6IN88US=> NVLBPFJYW0GQR1XS
human RPMI-6666 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnPOTY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLU[2OlYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ2M{e5JO69VQ>? NFfhOmFUSU6JRWK=
human OVCAR-3 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1r6[2lvcGmkaYTpc44hd2ZiaIXtZY4hV1[FQWKtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPjd7NE[g{txO M3fSbnNCVkeHUh?=
human NALM-6 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2\wWGlvcGmkaYTpc44hd2ZiaIXtZY4hVkGOTT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44ODR4NTFOwG0> MmPmV2FPT0WU
human RS4-11 cell M4LVbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MY\Jcohq[mm2aX;uJI9nKGi3bXHuJHJUPC1zMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|M5ODZizszN M{X3[XNCVkeHUh?=
human SK-MES-1 cell M3fzN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYPJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE44QDl4NzFOwG0> MnzVV2FPT0WU
human ES7 cell NE\mXm1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUHJcohq[mm2aX;uJI9nKGi3bXHuJGVUPyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOEGyPFYh|ryP NXPR[2pxW0GQR1XS
human NCI-H2342 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXjneXB1UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI{PDJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki0Nlk1KM7:TR?= NFfR[HRUSU6JRWK=
human SCC-9 cell MlT0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXzJcohq[mm2aX;uJI9nKGi3bXHuJHNESy17IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64OVc4KM7:TR?= NEP2NVdUSU6JRWK=
human EW-7 cell MlfpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGq4dHZKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg3OzV4IN88US=> MW\TRW5ITVJ?
human HH cell NGfWXFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHJcohq[mm2aX;uJI9nKGi3bXHuJGhJKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC57NkKyNkDPxE1? M{jxPXNCVkeHUh?=
human A427 cell NI\5SmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4n4T2lvcGmkaYTpc44hd2ZiaIXtZY4hSTR{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVk2ODVizszN M{TTW3NCVkeHUh?=
human Daudi cell NFfmbXhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVq1WZVmUW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODZ6OEig{txO MnjzV2FPT0WU
human P30-OHK cell NXnZSnNsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHzZcHFKdmirYnn0bY9vKG:oIHj1cYFvKFB|MD3PTGsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjB5MUO4JO69VQ>? M3fZfXNCVkeHUh?=
human HGC-27 cell NWPTdncyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlXMTY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVEvOThzNjFOwG0> NFq3VJBUSU6JRWK=
human CAMA-1 cell NXfmOGxqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3PXN2lvcGmkaYTpc44hd2ZiaIXtZY4hS0GPQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zODBzIN88US=> NV3Oco9RW0GQR1XS
human SK-OV-3 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHjDXlJKdmirYnn0bY9vKG:oIHj1cYFvKFONLV;WMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjJyNES0JO69VQ>? NF;OUlBUSU6JRWK=
human NCI-H1048 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3i4fGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixNFQ5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5{MkW3PUDPxE1? NEfINVBUSU6JRWK=
human MEL-HO cell M3jpZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHHXO41KdmirYnn0bY9vKG:oIHj1cYFvKE2HTD3IU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOjR{M{ig{txO MXrTRW5ITVJ?
human NKM-1 cell M{X2e2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGe0UmFKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zQDZ|NjFOwG0> NGTnZY9USU6JRWK=
human NCI-H650 cel M1HjNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWjJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNkWwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODB5NjFOwG0> MnvsV2FPT0WU
human HAL-01 cell NX3uZldnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3LOXmlvcGmkaYTpc44hd2ZiaIXtZY4hUEGOLUCxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODF5NTFOwG0> MY\TRW5ITVJ?
human 786-0 cell NX7mWFR7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWnJcohq[mm2aX;uJI9nKGi3bXHuJFc5Pi1yIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zNVI5OyEQvF2= NEDrSolUSU6JRWK=
human GI-1 cell NXG3UWNFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlrjTY5pcWKrdHnvckBw\iCqdX3hckBIUS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zNVgxQCEQvF2= NW\4cGRVW0GQR1XS
human Becker cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYjsRpF1UW6qaXLpeIlwdiCxZjDoeY1idiCEZXPr[ZIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjN4M{G1JO69VQ>? NYrx[VRxW0GQR1XS
human CCF-STTG1 cell NUKwfphXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4TxWmlvcGmkaYTpc44hd2ZiaIXtZY4hS0OILWPUWGcyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS52MkWyOUDPxE1? NIjUb5ZUSU6JRWK=
human MC116 cell M3K2bGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4W0PWlvcGmkaYTpc44hd2ZiaIXtZY4hVUNzMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlQ3OTJ{IN88US=> NIq1WnpUSU6JRWK=
human MDA-MB-468 cell NH3FeGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUDCSpJ{UW6qaXLpeIlwdiCxZjDoeY1idiCPRFGtUWIuPDZ6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT61N|c{PiEQvF2= NWHCVlN4W0GQR1XS
human NB12 cell M2TscWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlzhTY5pcWKrdHnvckBw\iCqdX3hckBPSjF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT61OFg1OiEQvF2= Ml;jV2FPT0WU
human LXF-289 cell MmPHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MknNTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU42PTFyNDFOwG0> MmTIV2FPT0WU
human MFE-296 cell NUe2OFZ7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVy3WGlKUW6qaXLpeIlwdiCxZjDoeY1idiCPRlWtNlk3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS53NkizOkDPxE1? NFP5b3dUSU6JRWK=
human SNU-423 cell MofkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{jE[mlvcGmkaYTpc44hd2ZiaIXtZY4hW06XLUSyN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTdzOUKg{txO MUjTRW5ITVJ?
human NCI-H2291 cell NIL2RmpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4XqemlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNlkyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS53N{[xJO69VQ>? M4\3fHNCVkeHUh?=
human OCUB-M cell MkS4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2D6emlvcGmkaYTpc44hd2ZiaIXtZY4hV0OXQj3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU42QDNzNDFOwG0> NWHkPY9LW0GQR1XS
human KU812 cell M4XrRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWrTNGNtUW6qaXLpeIlwdiCxZjDoeY1idiCNVUixNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTl4Mkig{txO NHfSemlUSU6JRWK=
human HuO9 cell M1rRTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoX3TY5pcWKrdHnvckBw\iCqdX3hckBJfU97IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62NVI5KM7:TR?= MVfTRW5ITVJ?
human 639-V cell NGnrSGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJFY{QS2YIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|E1PCEQvF2= M1zqdnNCVkeHUh?=
human HCC70 cell NFPTNW9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHJcohq[mm2aX;uJI9nKGi3bXHuJGhESzdyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62OlE1PiEQvF2= NXPQcHVPW0GQR1XS
human SNB75 cell NWLJcHF5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX;DbopPUW6qaXLpeIlwdiCxZjDoeY1idiCVTlK3OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjZ3MEig{txO MlrVV2FPT0WU
human D-336MG cell Ml\JS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NELFbmNKdmirYnn0bY9vKG:oIHj1cYFvKERvM{O2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjZ4OEig{txO NUnLUJVJW0GQR1XS
human LoVo cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEDldVdKdmirYnn0bY9vKG:oIHj1cYFvKEyxVn:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlc{QDJ3IN88US=> M1P5NnNCVkeHUh?=
human EB2 cell NYq1dotlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlHmTY5pcWKrdHnvckBw\iCqdX3hckBGSjJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLke1NFE6KM7:TR?= NYTDcpFbW0GQR1XS
human HSC-2 cell M{nY[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlK3TY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuO|U4OjdizszN Mk\qV2FPT0WU
human D-542MG cell NYntZopvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnLxTY5pcWKrdHnvckBw\iCqdX3hckBFNTV2Ml3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45PTB5NzFOwG0> NYDENYVqW0GQR1XS
human COLO-684 cell NFHJeJNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3fxcWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22PFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjJyMEOyJO69VQ>? M4Hld3NCVkeHUh?=
human GDM-1 cell M4nGZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVzJcohq[mm2aX;uJI9nKGi3bXHuJGdFVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6yOFIxPiEQvF2= NInId5pUSU6JRWK=
human SW1088 cell M3nPUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmLkTY5pcWKrdHnvckBw\iCqdX3hckBUXzFyOEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlQ5OTR|IN88US=> MVjTRW5ITVJ?
human SF295 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn7OTY5pcWKrdHnvckBw\iCqdX3hckBUTjJ7NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuO|Y6PTVizszN NGHkSnVUSU6JRWK=
human NCI-H2030 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkjiTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwN|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjlyMki4JO69VQ>? NGLsfWFUSU6JRWK=
human NCI-H1755 cell MlnNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{HIfGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixO|U2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{N{C2N{DPxE1? MmTIV2FPT0WU
human MDA-MB-361 cell NVjyeXlJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWPJcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj2zOlEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjV{N{C5JO69VQ>? NXH1cpNRW0GQR1XS

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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Assay of PI3K enzyme inhibition:

The inhibition of PI3Kβ, PI3Kα, PI3Kγ, and PI3Kδ is evaluated in an AlphaScreen based enzyme activity assay using human recombinant enzymes. The assay measures PI3K-mediated conversion of PIP2 to PIP3. Biotinylated PIP3, a GST-tagged pleckstrin homology (PH) domain and the two AlphaScreen beads form a complex that elicits a signal upon laser excitation at 680 nm. The PIP3 formed in the enzyme reaction competes with the biotinylated PIP3 for binding to the PH domain thus reducing the signal with increasing enzyme product. The AZD6482 is dissolved in DMSO and added to 384 well plates. PBKβ, PBKα, PBKγ, or PBKδ is added in a Tris buffer (50 mM Tris pH 7.6, 0.05% CHAPS, 5 mM DTT, and 24 mM MgCl2) and allowed to preincubate with AZD6482 for 20 minutes prior to the addition of substrate solution containing PIP2 and ATP. The enzyme reaction is stopped after 20 minutes by addition of stop solution containing EDTA and biotin-PIP3, followed by addition of detection solution containing GST-grpl PH and AlphaScreen beads. Plates are left for a minimum of 5 hours in the dark prior to analysis. The final concentration of DMSO, ATP and PIP2 in the assay are, 0.8%, 4 μM, and 40 μM, respectively. IC50 values are calculated according to the equation, y = {a+[(b-a)/(l+(x/IC50)s)]}, where y = % inhibition; a = 0%; b = 100%; s = the slope of the concentration-response curve; x = AZD6482 concentration.
Cell Research:[1]
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  • Cell lines: Human platelet pellet
  • Concentrations: 0–60 nM, dissolved in DMSO
  • Incubation Time: 5 min
  • Method: For assay of washed platelet aggregation (WPA), the platelet pellets are isolated from human blood and re-suspended to 2 × 1015/L in Tyrodes buffer (TB) containing 1 μM hirudin and 0.02 U/mL apyrase. Then, the platelet suspension is left to rest at room temperature for 30 min. Just prior to time for assay, CaCl2 is added to a final concentration of 2 mM. AZD6482, dissolved in DMSO, is added to a 96-well plate prior to the addition of the washed platelet suspension. The platelet suspension is preincubated with AZD6482 for 5 min. Light absorption at 650 nm is recorded before and after a 5 min plate shake and referred to as recording 0 (R0) and Rl. A mouse anti-human CD9 antibody is added (at a donor specific concentration) to each well prior to next 10 min plate shake and light absorption recording; R2. For data analysis, light absorbance in wells with TB are subtracted from all readings before percent aggregation is calculated according the formula: [(R1-R2)/R1] × 100 = % aggregation. Spontaneous aggregation or pro-aggregatory effect of the inhibitor is evaluated by the same formula, [(R0-Rl)/R0] × 100 = % aggregation. IC50 values are calculated according to the equation, y = {a+[(b-a)/(l+(x/IC50)s)]}, where y = % inhibition; a = 0%; b = 100%; s = the slope of the concentration-response curve; x = AZD6482 concentration.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 82 mg/mL (200.75 mM)
Ethanol 10 mg/mL (24.48 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 408.45
Formula

C22H24N4O4

CAS No. 1173900-33-8
Storage powder
in solvent
Synonyms KIN-193

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID