AZD6482

Catalog No.S1462 Synonyms: KIN-193

AZD6482 Chemical Structure

Molecular Weight(MW): 408.45

AZD6482 is a PI3Kβ inhibitor with IC50 of 10 nM, 8-, 87- and 109-fold more selective to PI3Kβ than PI3Kδ, PI3Kα and PI3Kγ in cell-free assays. Phase 1.

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Cited by 13 Publications

4 Customer Reviews

  • Cells treated with either control or INPP4B siRNA and following a 1-hour treatment with either DMSO or 1 mmol/L inhibitor; pPRAS40T246 and total PRAS40 were run on a separate blot for which the bottom Ku-86 panel is the loading control(pan-PI3Ki: GDC-0941, PI3K-ai: BYL719,PI3K-bi: AZD6482)

    Mol Cancer Res, 2017, 15(6):765-775. AZD6482 purchased from Selleck.

  • Dose response curve of compound on melanoma cell lines.  Compound was dissolved in DMSO, added in a 5-fold dilution series, starting with 10 μM, and incubated for 72 hours.  Fluorescence was measured after 8 hours incubation in resazurin.  Data was normalized to DMSO (maximal viability) and Doxorubicin (minimum viability).

    One customer. AZD6482 purchased from Selleck.

  • After starved in serum-free medium for 24h,A549 cells incubated with the indicated concentrations of AZD6482 for 3h,followed by 20-minute stimolation of 100ng/ml EGF.

    Dr. Zhang of Tianjin Medical University. AZD6482 purchased from Selleck.

  • Collagen-embedded melanoma spheroids were treated for 72 h with SGI-1776 [5 μM] or AZD6482 [10 μM] as single agents or in combination before staining for live (green) and dead (red) cells. Experiments were conducted in triplicate and representative images are shown. Scale bar represents 150 microns.

    Oncotarget, 2016, 7(34):54897-54912. AZD6482 purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description AZD6482 is a PI3Kβ inhibitor with IC50 of 10 nM, 8-, 87- and 109-fold more selective to PI3Kβ than PI3Kδ, PI3Kα and PI3Kγ in cell-free assays. Phase 1.
Targets
PI3Kβ [1]
(Cell-free assay)
PI3Kδ [1]
(Cell-free assay)
DNA-PK [1]
(Cell-free assay)
PI3Kα [1]
(Cell-free assay)
10 nM 80 nM 420 nM 870 nM
In vitro

AZD6482 also inhibits PI3Kα, γ, and δ, with IC50 of 80 nM to 1.09 μM, which are significantly lower than its (+)-enantiomer (S-form). AZD6482 is an antiplatelet agent; it blocks platelet activation adhesion/aggregation and promotes platelet disaggregation in assay of washed platelet aggregation (WPA), with an IC50 value of 6 nM. Furthermore, by targeting PI3Kβ, AZD6482 specifically inhibits thrombosis without interfering with normal haemostasis. Therefore, AZD6482 is used as an anti-thrombotic drug for the prophylaxis of thrombotic disorders. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human RXF393 cell NFrNTFRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkSxTY5pcWKrdHnvckBw\iCqdX3hckBTYEZ|OUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlAyOTV2IN88US=> MWrTRW5ITVJ?
human SW982 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MluwTY5pcWKrdHnvckBw\iCqdX3hckBUXzl6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNFM2QDRizszN MYnTRW5ITVJ?
human MAD-MB-468 cells NF7BTYxHfW6ldHnvckBie3OjeR?= NUniU3U3UW6qaXLpeIlwdiCxZjDQTVNM[mW2YTDpckBpfW2jbjDNRWQuVUJvNE[4JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiU3XyOFc{KEGtdDDwbI9{eGixconsZZRqd25iYomgZ4VtdHWuYYKgdI91\W6leTDhd5NigSxiSVO1NF0xNjB2IN88US=> MkfnNlQ6QTJ6N{S=
human KURAMOCHI cell NWnsdGdyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3e1cGlvcGmkaYTpc44hd2ZiaIXtZY4hU1WUQV3PR2hKKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zMEWxJO69VQ>? NHX6eZVUSU6JRWK=
human A498 cell M3fBcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2r1NmlvcGmkaYTpc44hd2ZiaIXtZY4hSTR7ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVE4QTVizszN MWnTRW5ITVJ?
human YT cell NIPSUFlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVztOlFYUW6qaXLpeIlwdiCxZjDoeY1idiC\VDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNVIxPjZizszN MV;TRW5ITVJ?
human VMRC-RCZ cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJHZOWkNvUlPaJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4yPDh7ODFOwG0> MnzHV2FPT0WU
human MDA-MB-415 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mlz4TY5pcWKrdHnvckBw\iCqdX3hckBOTEFvTVKtOFE2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zNkezNkDPxE1? M3\TU3NCVkeHUh?=
human J82 cell MkDpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUPJcohq[mm2aX;uJI9nKGi3bXHuJGo5OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNEe3O|kh|ryP NWP0O4JiW0GQR1XS
human SW1710 cell MnSzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEDVb4hKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPTJ7MjFOwG0> NHS3VWRUSU6JRWK=
human T47D cell NIPmW2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3XNdGlvcGmkaYTpc44hd2ZiaIXtZY4hXDR5RDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOVMzPyEQvF2= NEL4XYtUSU6JRWK=
human SU-DHL-1 cell NYrHVodzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYTF[ZVzUW6qaXLpeIlwdiCxZjDoeY1idiCVVT3ETGwuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNU[4NVMh|ryP MknnV2FPT0WU
human BT-20 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkHVTY5pcWKrdHnvckBw\iCqdX3hckBDXC1{MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOVkzOjJizszN MYPTRW5ITVJ?
human OS-RC-2 cell NWPuc4I6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF\uOndKdmirYnn0bY9vKG:oIHj1cYFvKE:VLWLDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZ{M{i4JO69VQ>? NFKyZmlUSU6JRWK=
human RPMI-6666 cell NF\3eHZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4Lid2lvcGmkaYTpc44hd2ZiaIXtZY4hWlCPST22OlY3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC54NEO3PUDPxE1? NFHkcmNUSU6JRWK=
human OVCAR-3 cell NGPxepFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Moj6TY5pcWKrdHnvckBw\iCqdX3hckBQXkODUj2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE43Pzl2NjFOwG0> NF\0bndUSU6JRWK=
human NALM-6 cell M2DUOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVvJcohq[mm2aX;uJI9nKGi3bXHuJG5CVE1vNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|A1PjVizszN NUO4R4NvW0GQR1XS
human RS4-11 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MorkTY5pcWKrdHnvckBw\iCqdX3hckBTWzRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlc{QDB4IN88US=> NHXRSVlUSU6JRWK=
human SK-MES-1 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIO5d|RKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FV{0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55OEm2O{DPxE1? MYXTRW5ITVJ?
human ES7 cell M1\Fc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVf5dlY1UW6qaXLpeIlwdiCxZjDoeY1idiCHU{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlgyOjh4IN88US=> MVjTRW5ITVJ?
human NCI-H2342 cell NYTEbGJqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{[yTGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyN|QzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC56NEK5OEDPxE1? MkLoV2FPT0WU
human SCC-9 cell NH3lZYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2HTdmlvcGmkaYTpc44hd2ZiaIXtZY4hW0OFLUmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg2PzdizszN M2\4fHNCVkeHUh?=
human EW-7 cell NH3m[|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVPJcohq[mm2aX;uJI9nKGi3bXHuJGVYNTdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLki2N|U3KM7:TR?= MX3TRW5ITVJ?
human HH cell MkfCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUnJcohq[mm2aX;uJI9nKGi3bXHuJGhJKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC57NkKyNkDPxE1? Mm\jV2FPT0WU
human A427 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUPacm5VUW6qaXLpeIlwdiCxZjDoeY1idiCDNEK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46QTVyNTFOwG0> MoTDV2FPT0WU
human Daudi cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX\GVGZoUW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODZ6OEig{txO M{XLUXNCVkeHUh?=
human P30-OHK cell NYfyeWo5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYDyV|FsUW6qaXLpeIlwdiCxZjDoeY1idiCSM{CtU2hMKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5yN{GzPEDPxE1? NYrQfFhQW0GQR1XS
human HGC-27 cell NHzJOHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoHUTY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVEvOThzNjFOwG0> MkK2V2FPT0WU
human CAMA-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYLmbJczUW6qaXLpeIlwdiCxZjDoeY1idiCFQV3BMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjJyMEGg{txO NXXQcphNW0GQR1XS
human SK-OV-3 cell M2KxSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIKzcVVKdmirYnn0bY9vKG:oIHj1cYFvKFONLV;WMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjJyNES0JO69VQ>? MnPxV2FPT0WU
human NCI-H1048 cell NIezd4hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUHvXIx5UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFExPDhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKyOVc6KM7:TR?= NVnDS|hbW0GQR1XS
human MEL-HO cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M372b2lvcGmkaYTpc44hd2ZiaIXtZY4hVUWOLVjPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zPDJ|ODFOwG0> MlfRV2FPT0WU
human NKM-1 cell NHTLc4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoLzTY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlg3OzZizszN NGe2O4RUSU6JRWK=
human NCI-H650 cel NFfVPVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUK3e5I2UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFY2OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwM{CwO|Yh|ryP M{jZVXNCVkeHUh?=
human HAL-01 cell NUDPcmgxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4XJS2lvcGmkaYTpc44hd2ZiaIXtZY4hUEGOLUCxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODF5NTFOwG0> MneyV2FPT0WU
human 786-0 cell NFX6UmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzpTY5pcWKrdHnvckBw\iCqdX3hckA4QDZvMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|EzQDNizszN MU\TRW5ITVJ?
human GI-1 cell M{fYcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXq4XGJtUW6qaXLpeIlwdiCxZjDoeY1idiCJST2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{OThyODFOwG0> NGG3[W1USU6JRWK=
human Becker cell MoHES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NG\qT5RKdmirYnn0bY9vKG:oIHj1cYFvKEKnY3vldkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzZ|MUWg{txO MYLTRW5ITVJ?
human CCF-STTG1 cell MoDlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2\NTGlvcGmkaYTpc44hd2ZiaIXtZY4hS0OILWPUWGcyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS52MkWyOUDPxE1? MVXTRW5ITVJ?
human MC116 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4X6bGlvcGmkaYTpc44hd2ZiaIXtZY4hVUNzMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlQ3OTJ{IN88US=> NVLSOWNkW0GQR1XS
human MDA-MB-468 cell NHnQUopIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXXJcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj20Olgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjV|N{O2JO69VQ>? MX;TRW5ITVJ?
human NB12 cell MnP2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1i1SGlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVQ5PDJizszN NHjG[lRUSU6JRWK=
human LXF-289 cell NVTIZZpZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M37tSGlvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTVzMESg{txO NViyfJdCW0GQR1XS
human MFE-296 cell M4m0Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVnlOHVFUW6qaXLpeIlwdiCxZjDoeY1idiCPRlWtNlk3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS53NkizOkDPxE1? NUTaXlBtW0GQR1XS
human SNU-423 cell NGfuN|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF3neXRKdmirYnn0bY9vKG:oIHj1cYFvKFOQVT20NlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjV5MUmyJO69VQ>? MlHXV2FPT0WU
human NCI-H2291 cell NGrQblVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoTZTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKyPVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjV5NkGg{txO NGT0botUSU6JRWK=
human OCUB-M cell M{fibmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1HsO2lvcGmkaYTpc44hd2ZiaIXtZY4hV0OXQj3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU42QDNzNDFOwG0> M4LwVHNCVkeHUh?=
human KU812 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWPJcohq[mm2aX;uJI9nKGi3bXHuJGtWQDF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT61PVYzQCEQvF2= M{TUcnNCVkeHUh?=
human HuO9 cell MmjTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MULJcohq[mm2aX;uJI9nKGi3bXHuJGh2VzliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLk[xNlgh|ryP M2foNHNCVkeHUh?=
human 639-V cell NWHvSotyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4LIPGlvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY{OTR2IN88US=> M3PlWXNCVkeHUh?=
human HCC70 cell NFvwb2lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1vxUmlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFN{CgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY3OTR4IN88US=> MXTTRW5ITVJ?
human SNB75 cell MmP4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWXJcohq[mm2aX;uJI9nKGi3bXHuJHNPSjd3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62OlUxQCEQvF2= MWHTRW5ITVJ?
human D-336MG cell NWPrOI9vT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHfNNHlKdmirYnn0bY9vKG:oIHj1cYFvKERvM{O2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjZ4OEig{txO NIe0OoxUSU6JRWK=
human LoVo cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoG4TY5pcWKrdHnvckBw\iCqdX3hckBNd1[xIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63N|gzPSEQvF2= NVO0TphVW0GQR1XS
human EB2 cell NI\uU|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2DtbGlvcGmkaYTpc44hd2ZiaIXtZY4hTUJ{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63OVAyQSEQvF2= M2\KW3NCVkeHUh?=
human HSC-2 cell NGHPcWNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M132SGlvcGmkaYTpc44hd2ZiaIXtZY4hUFOFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlc2PzJ5IN88US=> M2mxe3NCVkeHUh?=
human D-542MG cell NXy0b4QxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTR{TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlg2ODd5IN88US=> MmHLV2FPT0WU
human COLO-684 cell NH3CdIRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYDqXGs3UW6qaXLpeIlwdiCxZjDoeY1idiCFT1zPMVY5PCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMkCwN|Ih|ryP NV[0TYt2W0GQR1XS
human GDM-1 cell MnK0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1fvOGlvcGmkaYTpc44hd2ZiaIXtZY4hT0SPLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlI1OjB4IN88US=> NYrXOHZJW0GQR1XS
human SW1088 cell M3;sfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJHNYOTB6ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuOFgyPDNizszN NFvufZpUSU6JRWK=
human SF295 cell NUjMXY5NT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWnJcohq[mm2aX;uJI9nKGi3bXHuJHNHOjl3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj63Olk2PSEQvF2= NHTUSmNUSU6JRWK=
human NCI-H2030 cell M{D1OWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{K0cWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFMxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi57MEK4PEDPxE1? MonWV2FPT0WU
human NCI-H1755 cell MoruS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe1OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvOjdyNkOg{txO NFvES4FUSU6JRWK=
human MDA-MB-361 cell M{XoU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXT3d5BXUW6qaXLpeIlwdiCxZjDoeY1idiCPRFGtUWIuOzZzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz61NlcxQSEQvF2= NVfIS453W0GQR1XS

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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Assay of PI3K enzyme inhibition:

The inhibition of PI3Kβ, PI3Kα, PI3Kγ, and PI3Kδ is evaluated in an AlphaScreen based enzyme activity assay using human recombinant enzymes. The assay measures PI3K-mediated conversion of PIP2 to PIP3. Biotinylated PIP3, a GST-tagged pleckstrin homology (PH) domain and the two AlphaScreen beads form a complex that elicits a signal upon laser excitation at 680 nm. The PIP3 formed in the enzyme reaction competes with the biotinylated PIP3 for binding to the PH domain thus reducing the signal with increasing enzyme product. The AZD6482 is dissolved in DMSO and added to 384 well plates. PBKβ, PBKα, PBKγ, or PBKδ is added in a Tris buffer (50 mM Tris pH 7.6, 0.05% CHAPS, 5 mM DTT, and 24 mM MgCl2) and allowed to preincubate with AZD6482 for 20 minutes prior to the addition of substrate solution containing PIP2 and ATP. The enzyme reaction is stopped after 20 minutes by addition of stop solution containing EDTA and biotin-PIP3, followed by addition of detection solution containing GST-grpl PH and AlphaScreen beads. Plates are left for a minimum of 5 hours in the dark prior to analysis. The final concentration of DMSO, ATP and PIP2 in the assay are, 0.8%, 4 μM, and 40 μM, respectively. IC50 values are calculated according to the equation, y = {a+[(b-a)/(l+(x/IC50)s)]}, where y = % inhibition; a = 0%; b = 100%; s = the slope of the concentration-response curve; x = AZD6482 concentration.
Cell Research:[1]
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  • Cell lines: Human platelet pellet
  • Concentrations: 0–60 nM, dissolved in DMSO
  • Incubation Time: 5 min
  • Method: For assay of washed platelet aggregation (WPA), the platelet pellets are isolated from human blood and re-suspended to 2 × 1015/L in Tyrodes buffer (TB) containing 1 μM hirudin and 0.02 U/mL apyrase. Then, the platelet suspension is left to rest at room temperature for 30 min. Just prior to time for assay, CaCl2 is added to a final concentration of 2 mM. AZD6482, dissolved in DMSO, is added to a 96-well plate prior to the addition of the washed platelet suspension. The platelet suspension is preincubated with AZD6482 for 5 min. Light absorption at 650 nm is recorded before and after a 5 min plate shake and referred to as recording 0 (R0) and Rl. A mouse anti-human CD9 antibody is added (at a donor specific concentration) to each well prior to next 10 min plate shake and light absorption recording; R2. For data analysis, light absorbance in wells with TB are subtracted from all readings before percent aggregation is calculated according the formula: [(R1-R2)/R1] × 100 = % aggregation. Spontaneous aggregation or pro-aggregatory effect of the inhibitor is evaluated by the same formula, [(R0-Rl)/R0] × 100 = % aggregation. IC50 values are calculated according to the equation, y = {a+[(b-a)/(l+(x/IC50)s)]}, where y = % inhibition; a = 0%; b = 100%; s = the slope of the concentration-response curve; x = AZD6482 concentration.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 82 mg/mL (200.75 mM)
Ethanol 10 mg/mL (24.48 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 408.45
Formula

C22H24N4O4

CAS No. 1173900-33-8
Storage powder
in solvent
Synonyms KIN-193

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID