VPS34-IN1

Catalog No.S7980 Synonyms: Vps34-IN-1

For research use only.

Vps34-IN1 is a potent and highly selective Vps34 inhibitor with IC50 of 25 nM invitro,which does not significantly inhibit the isoforms of class I as well as class II PI3Ks. Vps34-IN1 modulates autophagy.

VPS34-IN1 Chemical Structure

CAS No. 1383716-33-3

Selleck's VPS34-IN1 has been cited by 21 publications

Purity & Quality Control

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Biological Activity

Description Vps34-IN1 is a potent and highly selective Vps34 inhibitor with IC50 of 25 nM invitro,which does not significantly inhibit the isoforms of class I as well as class II PI3Ks. Vps34-IN1 modulates autophagy.
Targets
Vps34 [1]
(Cell-free assay)
25 nM
In vitro

Administration of VPS34-IN1 to cells induces a rapid dose-dependent dispersal of a specific PtdIns(3)P-binding probe from endosome membranes, within 1min, without affecting the ability of class I PI3K to regulate Akt. It can also induce a rapid ~50-60% loss of SGK3 phosphorylation within 1min. VPS34-IN1 has no inhibition to the SGK2 isoform that does not possess a PtdIns(3)P-binding PX domain[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
VERO-E6 NH7DeXJHfW6ldHnvckBie3OjeR?= M{\DZ|Q5KGi{cx?= MV\E[ZRmem2rbnH0bY9vKG:oIFnDOVAhfmGudXXzJIZweiCrbnjpZol1cW:wIH;mJHNCWlNvQ3;WMVIhcW6mdXPl[EBkgXSxdH;4bYNqfHlib3[gWmVTVy2HNjDj[YxteyCjZoTldkA1QCCqb4Xyd{Ah\Xiyb4P1doUhfG9iMD6wNUBOV0liU1HSV{BEd1ZvMjD2bZJ2eyCkeTDobYdpKGOxboTlcpQhcW2jZ3nu[{whUUN3ME2wMlTPxE1? NEDSOlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{QTF|N{S2M{c,S2iHTVLMQE9iRg>?
VERO-E6 NV3VTVFJXG:6aXPpeJkh[XO|YYm= MYC0PEBpenN? NXzEdIY4XG:6aXPpeJkhS0N3MDDh[4FqdnO2IG\FVm8uTTZiY3XscJMh\GW2ZYLtbY5m\CCjdDC0PEBpd3W{czDifUBpcWeqIHPvcpRmdnRiaX3h[4lv\yBqc3Ht[UBkd26maYTpc45{KGG|IELfUGV[KHerdHjveZQh\Xiyb4P1doUhfG9iMD6wNUBOV0liU1HSV{BEd1ZvMjD2bZJ2eyluIFPDOVA:OjZwOEROwG0> MnzhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|kyOzd2Nj:nQmNpTU2ETEyvZV4>
Assay
Methods Test Index PMID
Western blot p-SGK3 / SGK3 ; Nanog / CD133 25177796 29940988
Growth inhibition assay Cell viability 31515514

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 425.91
Formula

C21H24ClN7O

CAS No. 1383716-33-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(CNC1=NC=C(C(=N1)CC2CC2)C3=NC(=NC=C3)NC4=CC(=NC=C4)Cl)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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