VPS34 inhibitor 1 (Compound 19)

For research use only.

Catalog No.S8456 Synonyms: PIK-III analogue

3 publications

VPS34 inhibitor 1 (Compound 19) Chemical Structure

CAS No. 1383716-46-8

VPS34 inhibitor 1 (Compound 19, PIK-III analogue) is a potent and selective inhibitor of VPS34 with an IC50 of 15 nM.

Selleck's VPS34 inhibitor 1 (Compound 19) has been cited by 3 publications

1 Customer Review

  • Confirmation of selective growth inhibition using VPS34-IN1 inhibitor. Proliferation of Tsc2+/+ and Tsc2-/- MEFs treated with vehicle control (DMSO), CQ (5 uM), VPS34-IN1 (500 nM) or the combination for 72 hours (crystal violet staining). Two sample t-test was carried out between CQ and SAR405 and each drug alone. Results are representative of three independent experiments. Bar graph represent means ±SD.

    Oncotarget, 2017, 8(24):38099-38112. VPS34 inhibitor 1 (Compound 19) purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description VPS34 inhibitor 1 (Compound 19, PIK-III analogue) is a potent and selective inhibitor of VPS34 with an IC50 of 15 nM.
VPS34 [1]
(Cell-free assay)
15 nM
In vitro

Compound 19 is extraordinarily selective over other lipid and protein kinases. The ability of compound 19 to prevent the degradation of autophagy substrates p62, NCOA4, NBR1, NDP52, and FTH1 is similar to PIK-III. In addition, treatment of cells with compound 19 leads to an increase in the lipidated and nonlipidated forms of LC3 similar to previous reports using PIK-III[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U2OS NXyyV|RITnWwY4Tpc44h[XO|YYm= NW\wUJJROiCqcoO= MULJcohq[mm2aX;uJI9nKF[SU{O0JIlvKGi3bXHuJHUzV1NiY3XscJMhcW6ldXLheIVlKG[xcjCyJIhzeyCkeTDHSnAuTlmYRTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNFI2|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjhzOU[2PUc,OjZ6MUm2Olk9N2F-
DLD1 NVjqWGNNTnWwY4Tpc44h[XO|YYm= NEX6[5UyKHSxIEGwJJVO M{XyeFI1KGi{cx?= NHHRUo1KdmirYnn0bY9vKG:oIG\QV|M1KGmwIHj1cYFvKESOREGgZ4VtdHNiYYPz[ZN{\WRiYYOgdJJmfmWwdHnvckBw\iCQQ1;BOEBxem:2ZXnuJIRm\3KjZHH0bY9vKGG2IEGgeI8hOTBidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M3zOVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEG5OlY6Lz5{NkixPVY3QTxxYU6=
DLD1 NIj3fHBHfW6ldHnvckBie3OjeR?= NWPwWpo2OSC2bzCxNEB2VQ>? NGLqXoczPCCqcoO= M1rmdGlvcGmkaYTpc44hd2ZiVmDTN|QhcW5iaIXtZY4hTEyGMTDj[YxteyCjc4Pld5Nm\CCjczDwdoV3\W62aX;uJI9nKHB4MjDk[Ydz[WSjdHnvckBifCBzIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjhzOU[2PUc,OjZ6MUm2Olk9N2F-
DLD1 MljESpVv[3Srb36gZZN{[Xl? Mn;PNUB1dyBzMDD1US=> M2joUVI1KGi{cx?= MoPDTY5pcWKrdHnvckBw\iCYUGOzOEBqdiCqdX3hckBFVERzIHPlcIx{KGG|c3Xzd4VlKGG|IIDy[ZZmdnSrb36gc4YhVkKUMTDwdo91\WmwIHTl[5Ji\GG2aX;uJIF1KDFidH:gNVAhfU1iaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M371RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEG5OlY6Lz5{NkixPVY3QTxxYU6=
DLD1 Mlj3SpVv[3Srb36gZZN{[Xl? NUnreIxuOSC2bzCxNEB2VQ>? Ml60NlQhcHK| MWfJcohq[mm2aX;uJI9nKF[SU{O0JIlvKGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNicILleoVvfGmxbjDv[kBPTFB3MjDwdo91\WmwIHTl[5Ji\GG2aX;uJIF1KDFidH:gNVAhfU1iaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NFXmOXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkixPVY3QSd-Mk[4NVk3Pjl:L3G+
DLD1 M4C5NWZ2dmO2aX;uJIF{e2G7 NHXzTpMyKHSxIEGwJJVO MVmyOEBpenN? M1fLVGlvcGmkaYTpc44hd2ZiVmDTN|QhcW5iaIXtZY4hTEyGMTDj[YxteyCjc4Pld5Nm\CCjczDwdoV3\W62aX;uJI9nKE[WSEGgdJJwfGWrbjDk[Ydz[WSjdHnvckBifCBzIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjhzOU[2PUc,OjZ6MUm2Olk9N2F-
DLD1 M{DOSmZ2dmO2aX;uJIF{e2G7 NYrQVmVuOSC2bzCxNEB2VQ>? MlLzNlQhcHK| MWHJcohq[mm2aX;uJI9nKF[SU{O0JIlvKGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gUGM{NTFicILveIVqdiCneIDy[ZN{cW:wIHH0JFEhfG9iMUCgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ Mkn4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6MUm2OlkoRjJ4OEG5OlY6RC:jPh?=
DLD1 M3nVXmZ2dmO2aX;uJIF{e2G7 NFyxTG0yKHSxIEGwJJVO Ml7ONlQhcHK| MWDJcohq[mm2aX;uJI9nKF[SU{O0JIlvKGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gUGM{NTJicILveIVqdiCneIDy[ZN{cW:wIHH0JFEhfG9iMUCgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M{XpRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEG5OlY6Lz5{NkixPVY3QTxxYU6=

... Click to View More Cell Line Experimental Data

In vivo The pharmacokinetic profile of analogue 19 is determined in C57BL/6 mice. After oral administration at 10 mg/kg, the compound is rapidly absorbed and showed moderate mean systemic clearance (30 mL/min/kg, approximately 33% of hepatic blood flow), with good oral bioavailability (F% = 47). Based on these PK parameters and the cellular activity, compound 19 constitutes a suitable candidate for in vivo studies. Upon oral administration of compound 19 at 50 mg/kg twice a day (BID) for 7 days, LC3-II accumulates consistent with reduced autophagic capacity in time-dependent manner. It inhibits autophagy in vivo[1].


Cell Research:[1]
- Collapse
  • Cell lines: U2OS cells
  • Concentrations: 0, 1, 5, 10 μM
  • Incubation Time: 24 h
  • Method: For inhibitor assay, cells are plated and the following day when cells had reached 90%, are treated with dimethyl sulfoxide (DMSO, vehicle) or the indicated concentration of PIK-III or Compound 19, both dissolved in DMSO. 24 hours later, cells are lysed in RIPA supplemented with 1% SDS and mini-EDTA protease inhibitors, homogenized by passage through a Qiashredder column and the protein is quantified by DC Lowry protein assay.
    (Only for Reference)
Animal Research:[1]
- Collapse
  • Animal Models: C57BL/6 Mice
  • Dosages: 10 mg/kg(p.o.) or 2 mg/kg(I.V.)
  • Administration: oral administration or I.V.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 78 mg/mL (199.24 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 391.47


CAS No. 1383716-46-8
Storage powder
in solvent
Synonyms PIK-III analogue
Smiles CC(C)(CNC1=NC=C(C(=N1)CC2CC2)C3=NC(=NC=C3)NC4=CC=NC=C4)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID