Romidepsin (FK228, Depsipeptide)
Catalog No.S3020 Synonyms: FR 901228, NSC 630176
Molecular Weight(MW): 540.7
Romidepsin (FK228, depsipeptide) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM in cell-free assays, respectively.
Cited by 73 Publications
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|Description||Romidepsin (FK228, depsipeptide) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM in cell-free assays, respectively.|
|Features||More effective than other classical HDAC inhibitors such as TSA, TPX, and butyrate.|
Unlike TSA, the active form redFK of Romidepsin strongly inhibits HDAC1 and HDAC2 with IC50 of 1.6 nM and 3.9 nM, respectively, but is relatively weak in inhibiting HDAC4 and HDAC6 with IC50 25 nM and 790 nM, respectively. Romidepsin is 17-23 times weaker than redFK in inhibiting these HDACs with IC50 of 36 nM, 47 nM, 510 nM, and 14 μM, respectively. Romidepsin treatment in HeLa cells induces histone acetylation and p21 expression with EC50 of 3.0 nM, more strongly than redFK with EC50 of 11 nM due to the instability of redFK.  In addition to G2/M arrest, Romidepsin treatment causes cyclin D1 downregulation and a p53-independent p21 induction, leading to inhibition of CDK and dephosphorylation of Rb resulting in growth arrest in the early G1 phase.  Romidepsin is 100 times more potent than TSA and 1,000,000 times more potent than butyrate in inhibiting the proliferation of the A549 cells.  Romidepsin inhibits the growth of U-937, K562, and CCRF-CEM cells with IC50 of 5.92 nM, 8.36 nM, and 6.95 nM, respectively.  Romidepsin promotes apoptosis in chronic lymphocytic leukemia (CLL) cells at a concentration corresponding to that at which H3 and H4 acetylation and HDAC inhibition occurs, selectively involving activation of caspase 8 and effector caspase 3, as well as down-regulation of c-FLIP protein.  In 11 of 13 (85%) renal cell carcinoma cell lines and in 16 of 37 (43%) other cancer cell lines, Romidepsin treatment up-regulates tumor death receptors, and potentiates natural killer (NK)-mediated tumor killing.  Romidepsin exhibits concentration-dependent cytotoxicity against a panel of mantle cell lymphoma (MCL) cell lines. 
|In vivo||Romidepsin treatment potently inhibits the neovascularization of chick embryo and that of adult mice in the Matrigel plug assay. Administration of Romidepsin at 0.1-1 mg/kg twice a week significantly prolongs the survival of mice bearing U-937 lymphoma, with median survival times of 30.5 (0.56 mg/kg) and 33 days (0.32 mg/kg), respectively (vs. 20 days in control mice). |
HDAC-inhibitory activity:For the enzyme assay, 10 μL of [3H]acetyl-labeled histones (25,000 cpm/10 μg) are added to 90 μL of the HDAC enzyme fraction extracted from 293T cells overexpressing HDAC1 or HDAC2 in the presence of increasing concentrations of Romidepsin, and the mixture is incubated at 37 °C for 15 minutes. The enzyme reaction is linear for at least 1 hour. The reaction is stopped by the addition of 10 μL of concentrated HCl. The released [3H]acetic acid is extracted with 1 mL of ethylacetate, and 0.9 mL of the solvent layer is taken into 5 mL of aqueous counting scintillant II solution for determination of radioactivity. The IC50 values are determined from at least three independent dose-response curves.
-  Furumai R, et al. Cancer Res, 2002, 62(17), 4916-4921.
-  Sandor V, et al. Br J Cancer, 2000, 83(6), 817-825.
-  Blagosklonny MV, et al. Mol Cancer Ther, 2002, 1(11), 937-941.
-  Kwon HJ, et al. Int J Cancer, 2002, 97(3), 290-296.
-  Sasakawa Y, et al. Biochem Pharmacol, 2002, 64(7), 1079-1090.
-  Aron JL, et al. Blood, 2003, 102(2), 652-628.
-  Lundqvist A, et al. Cancer Res, 2006, 66(14), 7317-7325.
-  Dai Y, et al. Clin Cancer Res, 2008, 14(2), 549-558.
-  Paoluzzi L, et al. Clin Cancer Res, 2010, 16(2), 554-565.
|In vitro||DMSO||10 mg/mL (18.49 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5%Tween 80+ddH2O
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||FR 901228, NSC 630176|
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03770000||Recruiting||Drug: Tenalisib|Drug: Romidepsin||T Cell Lymphoma||Rhizen Pharmaceuticals SA||March 12 2019||Phase 1|Phase 2|
|NCT02616965||Recruiting||Drug: Romidepsin|Drug: Brentuximab vedotin||Cutaneous T-cell Lymphoma (CTCL)||Fox Chase Cancer Center|Seattle Genetics Inc.|Celgene Corporation||February 22 2017||Phase 1|
|NCT02616874||Completed||Drug: MVA.HIVconsv vaccine|Drug: Romidepsin||HIV||IrsiCaixa|Germans Trias i Pujol Hospital|Fundacio Lluita Contra la SIDA|Hospital Clinic of Barcelona|Hospital de Sant Pau|HIVACAT|University of Oxford|BCN Checkpoint||February 2016||Phase 1|
|NCT03141203||Recruiting||Drug: Romidepsin|Drug: Carfilzomib||Peripheral T Cell Lymphoma||University of Birmingham|Bloodwise|Celgene|Amgen||July 13 2015||Phase 1|Phase 2|
|NCT02393794||Recruiting||Drug: Romidepsin|Drug: Cisplatin|Drug: Nivolumab||Triple-Negative Breast Cancer|Breast Cancer||Priyanka Sharma|Celgene Corporation|Bristol-Myers Squibb|University of Kansas Medical Center||July 17 2015||Phase 1|Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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