Sodium Phenylbutyrate

For research use only.

Catalog No.S4125 Synonyms: 4-PBA, 4-Phenylbutyric acid

13 publications

Sodium Phenylbutyrate Chemical Structure

CAS No. 1716-12-7

Sodium phenylbutyrate is a salt of 4-phenylbutyrate (4-PBA) or 4-phenylbutyric acid.Sodium phenylbutyrate is a histone deacetylase inhibitor, used to treat urea cycle disorders.

Selleck's Sodium Phenylbutyrate has been cited by 13 publications

1 Customer Review

  • Representative images and quantitative analysis results of the transwell experiment. Notes: (A) Results of migration assay for DU145. (B) Results of invasion assay for DU145. (C) Results of migration assay for PC3. (D) Results of invasion assay for PC3. “*” Means significantly different from control group, P<0.05. Abbreviation: SPB, sodium phenylbutyrate.

    OncoTargets and Therapy, 2016, 9:2825-2833.. Sodium Phenylbutyrate purchased from Selleck.

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Biological Activity

Description Sodium phenylbutyrate is a salt of 4-phenylbutyrate (4-PBA) or 4-phenylbutyric acid.Sodium phenylbutyrate is a histone deacetylase inhibitor, used to treat urea cycle disorders.
HDAC [1]
In vitro

Phenylbutyrate is a well-known HDAC inhibitor, which increases gene transcription of a number of genes, and also exerts neuroprotective effects. Phenylbutyrate significantly attenuates MPTP-induced depletion of striatal dopamine and loss of tyrosine hydroxylase-positive neurons in the substantia nigra. [1] Phenylbutyrate attenuates the expression of the apoptosis antagonist Bcl-X(L), the double-strand break repair protein DNA-dependent protein kinase, the prostate progression marker caveolin-1, and the pro-angiogenic vascular endothelial growth factor in prostate cancer cells. Phenylbutyrate is found to act in synergy with ionizing radiation to induce apoptosis in prostate cancer cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hela M16yRWZ2dmO2aX;uJIF{e2G7 MXrJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVt[SClZXzsd{BvfWOuZXHyJIV5fHKjY4TzJIJ6KG[udX;ybY1mfHKrYzDhd5NigSxiS3m9Ok4{PM7:TR?= NXXWcmJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NlA2QDBpPkG5OVIxPThyPD;hQi=>
U937 M2LmWmZ2dmO2aX;uJIF{e2G7 M2L5eVQhdU1? M3fkSFghfG9iNEigbJJ{ NFG3cotKdmS3Y4Tpc44hd2ZiQ1HNVEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gWVk{PyClZXzsd{BifCB2IH3NJIFnfGW{IEigeI8hPDhiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd5MEK3N{c,OTl5N{CyO|M9N2F-
VA10 MlG3SpVv[3Srb36gZZN{[Xl? NV[xbmsxPCCvTR?= NFuye4EzPCCqcoO= NFTvbo5KdmS3Y4Tpc44hd2ZiQ1HNVEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gWmEyOCClZXzsd{BifCB2IH3NJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd5MEK3N{c,OTl5N{CyO|M9N2F-
HT-29 MVPGeY5kfGmxbjDhd5NigQ>? MYO0JI1O NYTwbm1WQCC2bzC0PEBpenN? MW\JcoR2[3Srb36gc4YhS0GPUDDtVm5CKGW6cILld5Nqd25iaX6gbJVu[W5iSGStNlkh[2WubIOgZZQhPCCvTTDh[pRmeiB6IITvJFQ5KGi{czDifUBTXC2SQ2KgZY5idHm|aYO= NG\SXZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe3NFI4Oyd-MUm3O|AzPzN:L3G+
A498 NXPPW2dbTnWwY4Tpc44h[XO|YYm= M1XGZ|QhdU1? MVG4JJRwKDR6IHjydy=> MnjyTY5lfWO2aX;uJI9nKEODTWCgcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIFG0PVgh[2WubIOgZZQhPCCvTTDh[pRmeiB6IITvJFQ5KGi{czDifUBTXC2SQ2KgZY5idHm|aYO= M1HlUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{ewNlc{Lz5zOUe3NFI4OzxxYU6=
VA10 NV\w[5NMTnWwY4Tpc44h[XO|YYm= MlrWOEBuVQ>? NY\iO2puOjRiaILz M13qV2lv\HWldHnvckBw\iCGRV\CNUBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gWmEyOCClZXzsd{BifCB2IH3NJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? MlmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5N{CyO|MoRjF7N{ewNlc{RC:jPh?=
VA10 M2\yOWZ2dmO2aX;uJIF{e2G7 M131NVQhdU1? MoDHNlQhcHK| NWfKe|JQUW6mdXP0bY9vKG:oIFzMMVM4KHC{b4TlbY4h\XiycnXzd4lwdiCrbjDoeY1idiCYQUGwJINmdGy|IHH0JFQhdU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdITpcoc> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd5MEK3N{c,OTl5N{CyO|M9N2F-
VA10 NXLZ[oVrTnWwY4Tpc44h[XO|YYm= MYiyNEBvVQ>? MVqyOEBpenN? M2HvSWlv\HWldHnvckBw\iCOTD2zO{Bxem:2ZXnuJIV5eHKnc4Ppc44hcW5iaIXtZY4hXkFzMDDj[YxteyCjdDCyNEBvVSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[y=> NGjUb|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe3NFI4Oyd-MUm3O|AzPzN:L3G+

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot

PubMed: 27274278     

(A) SPB treatment remarkably inhibited the expression of survivin in both cell types. SPB, sodium phenylbutyrate.

p38 / p-p38 / ERK / p-ERK / JNK / p-JNK ; 

PubMed: 27274278     

(B) SPB treatment increased the phosphorylation of p38 and ERK, while it had no effect on the activity of JNK. SPB, sodium phenylbutyrate.

In vivo Phenylbutyrate significantly extends survival and improved both the clinical and neuropathological phenotypes in G93A transgenic ALS mice. Phenylbutyrate administration ameliorates histone hypoacetylation observed in G93A mice and induced expression of nuclear factor-kappaB (NF-kappaB) p50, the phosphorylated inhibitory subunit of NF-kappaB (pIkappaB) and beta cell lymphoma 2 (bcl-2), but reduced cytochrome c and caspase expression. Phenylbutyrate acts to phosphorylate IkappaB, translocating NF-kappaB p50 to the nucleus, or to directly acetylate NF-kappaB p50. [3] Phenylbutyrate increases brain histone acetylation and decreased histone methylation levels as assessed by both immunocytochemistry and Western blots in a transgenic mousemodel of Huntington's disease (HD). Phenylbutyrate increases mRNA for components of the ubiquitin-proteosomal pathway and down-regulated caspases implicated in apoptotic cell death, and active caspase 3 immunoreactivity in the striatum. [4]


Solubility (25°C)

In vitro Water 30 mg/mL (161.13 mM)
DMSO 8 mg/mL (42.96 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 186.18


CAS No. 1716-12-7
Storage powder
in solvent
Synonyms 4-PBA, 4-Phenylbutyric acid
Smiles C1=CC=C(C=C1)CCCC(=O)[O-].[Na+]

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05019417 Recruiting Drug: Glycerol Phenylbutyrate 1100 MG/ML Monocarboxylate Transporter 8 Deficiency Kaplan Medical Center|Weizmann Institute of Science June 30 2021 Phase 2|Phase 3
NCT04937062 Recruiting Drug: Glycerol Phenylbutyrate 1100 MG/ML [Ravicti] STXBP1 Encephalopathy With Epilepsy SLC6A1 Neurodevelopmental Disorder Weill Medical College of Cornell University March 1 2021 Early Phase 1
NCT04421677 Active not recruiting Drug: Phenylbutyrate Oral Tablet Inclusion Body Myositis|Sporadic Inclusion Body Myositis University of Kansas Medical Center August 20 2020 Phase 1
NCT02246218 Completed Drug: RAVICTI Urea Cycle Disorder Horizon Therapeutics LLC|Horizon Pharma Ireland Ltd. Dublin Ireland December 31 2014 Phase 4
NCT01096095 Withdrawn Drug: Placebo|Drug: Sodium Phenylbutyrate Spinocerebellar Ataxia Type 3 Hospital de Clinicas de Porto Alegre|Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul Brazil June 2010 Phase 2
NCT00947544 Completed Drug: HPN-100|Drug: NaPBA Urea Cycle Disorders Horizon Pharma Ireland Ltd. Dublin Ireland March 2010 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID