Home Epigenetics HDAC inhibitor NKL 22

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NKL 22 HDAC inhibitor

Cat.No.S6548

NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.
NKL 22 HDAC inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 325.40

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Quality Control

Batch: S654801 DMSO]65 mg/mL]false]Ethanol]3 mg/mL]false]Water]Insoluble]false Purity: 98.09%
98.09

Chemical Information, Storage & Stability

Molecular Weight 325.40 Formula

C19H23N3O2

 

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 537034-15-4 -- Storage of Stock Solutions

Synonyms N/A Smiles C1=CC=C(C=C1)NC(=O)CCCCCC(=O)NC2=CC=CC=C2N

Solubility

In vitro
Batch:

DMSO : 65 mg/mL (199.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Mechanism of Action

Targets/IC50/Ki
HDAC [1]
(Cell-free assay)
78 μM
In vitro

2.5 μM of NKL 22 increases frataxin protein concentrations in the FRDA ymphoid cell line. Incubation for 2 d in culture with this compound increases the FXN mRNA level in lymphocytes from the affected individual to about 80% of that in lymphocytes from the unaffected individual. This chemical is highly active in increasing FXN mRNA levels in cells obtained from both carrier C and affected AC, bringing the FXN mRNA level in the FRDA lymphocytes to about 160% of that found in the untreated carrier lymphocytes.[1].
References

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