NKL 22

Catalog No.S6548

For research use only.

NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.

NKL 22 Chemical Structure

CAS No. 537034-15-4

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Biological Activity

Description NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.
Targets
HDAC [1]
(Cell-free assay)
78 μM
In vitro

2.5 μM of NKL 22 increases frataxin protein concentrations in the FRDA ymphoid cell line. Incubation for 2 d in culture with NKL 22 increases the FXN mRNA level in lymphocytes from the affected individual to about 80% of that in lymphocytes from the unaffected individual. NKL 22 is highly active in increasing FXN mRNA levels in cells obtained from both carrier C and affected AC, bringing the FXN mRNA level in the FRDA lymphocytes to about 160% of that found in the untreated carrier lymphocytes.[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: human lymphocytes
  • Concentrations: 2.5 μM
  • Incubation Time: 48 h
  • Method:

    Blood is collected in heparinized Vacutainer tubes and lymphocytes are isolated by density centrifugation using Ficoll-Paque PLUS. Lymphocytes are maintained in the same culture medium and conditions as the established cell lines. HDAC inhibitors are dissolved in DMSO and added the solution to the culture medium at the concentrations, for the indicated times. The final DMSO concentration in the culture medium does not exceed 0.5% (v/v). Cells are treated with HDAC inhibitors after 16 h, and RNA is isolated after subsequent 48-h incubation.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 325.40
Formula

C19H23N3O2

 

CAS No. 537034-15-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)NC(=O)CCCCCC(=O)NC2=CC=CC=C2N

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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