NKL 22

For research use only.

Catalog No.S6548

NKL 22 Chemical Structure

CAS No. 537034-15-4

NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.

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Biological Activity

Description NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.
HDAC [1]
(Cell-free assay)
78 μM
In vitro

2.5 μM of NKL 22 increases frataxin protein concentrations in the FRDA ymphoid cell line. Incubation for 2 d in culture with NKL 22 increases the FXN mRNA level in lymphocytes from the affected individual to about 80% of that in lymphocytes from the unaffected individual. NKL 22 is highly active in increasing FXN mRNA levels in cells obtained from both carrier C and affected AC, bringing the FXN mRNA level in the FRDA lymphocytes to about 160% of that found in the untreated carrier lymphocytes.[1].


Cell Research:


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  • Cell lines: human lymphocytes
  • Concentrations: 2.5 μM
  • Incubation Time: 48 h
  • Method:

    Blood is collected in heparinized Vacutainer tubes and lymphocytes are isolated by density centrifugation using Ficoll-Paque PLUS. Lymphocytes are maintained in the same culture medium and conditions as the established cell lines. HDAC inhibitors are dissolved in DMSO and added the solution to the culture medium at the concentrations, for the indicated times. The final DMSO concentration in the culture medium does not exceed 0.5% (v/v). Cells are treated with HDAC inhibitors after 16 h, and RNA is isolated after subsequent 48-h incubation.

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 65 mg/mL (199.75 mM)
Ethanol 3 mg/mL (9.21 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 325.40



CAS No. 537034-15-4
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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HDAC Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID