Home Epigenetics HDAC inhibitor NKL 22 For research use only.

NKL 22

NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.

NKL 22 Chemical Structure

NKL 22 Chemical Structure

CAS: 537034-15-4

Purity & Quality Control

Batch: S654801 DMSO] 65 mg/mL] false] Ethanol] 3 mg/mL] false] Water] Insoluble] false Purity: 98.09%
98.09

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Biological Activity

Description NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.
Targets
HDAC [1]
(Cell-free assay)
78 μM
In vitro
In vitro

2.5 μM of NKL 22 increases frataxin protein concentrations in the FRDA ymphoid cell line. Incubation for 2 d in culture with NKL 22 increases the FXN mRNA level in lymphocytes from the affected individual to about 80% of that in lymphocytes from the unaffected individual. NKL 22 is highly active in increasing FXN mRNA levels in cells obtained from both carrier C and affected AC, bringing the FXN mRNA level in the FRDA lymphocytes to about 160% of that found in the untreated carrier lymphocytes.[1].
Cell Research Cell lines human lymphocytes
Concentrations 2.5 μM
Incubation Time 48 h
Method

Blood is collected in heparinized Vacutainer tubes and lymphocytes are isolated by density centrifugation using Ficoll-Paque PLUS. Lymphocytes are maintained in the same culture medium and conditions as the established cell lines. HDAC inhibitors are dissolved in DMSO and added the solution to the culture medium at the concentrations, for the indicated times. The final DMSO concentration in the culture medium does not exceed 0.5% (v/v). Cells are treated with HDAC inhibitors after 16 h, and RNA is isolated after subsequent 48-h incubation.

Chemical Information & Solubility

Molecular Weight 325.40 Formula

C19H23N3O2

 
CAS No. 537034-15-4 SDF --
Smiles C1=CC=C(C=C1)NC(=O)CCCCCC(=O)NC2=CC=CC=C2N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 65 mg/mL ( (199.75 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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