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Cyclopamine

Name: Cyclopamine
Brand: Selleck Chemicals
SKU: S1146
Rating: 5 (102 reviews)

Catalog No.S1146 Synonyms: 11-deoxojervine

For research use only.

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

CAS No. 4449-51-8

Selleck's Cyclopamine has been cited by 102 publications

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Hedgehog/Smoothened Signaling Pathway Map

Biological Activity

Description

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

Targets

Smoothened ^[1]
(TM3Hh12 cells)

46 nM

In vitro

Cyclopamine inhibits the Hedgehog signaling pathway with an IC50 of 46 nM, and blocks the activity of human Smo receptor expressed in CHO-K1 cells in [³H]Hh-Ag binding assay with an IC50 of 280 nM. ^[1] Cyclopamine significantly inhibits Hedgehog pathway activity in a dose-dependent manner in gut-derived tumor cell lines expressing Patched (PTCH) mRNA, and induces growth inhibition of those tumor cell lines by 75-95% at the concentration of 3 μM, but ineffective towards the colon tumor cells without PTCH mRNA expression, suggesting the effects of Cyclopamine treatment are Hedgehog pathway related rather than generally cytotoxic. ^[2] By blocking Hedgehog signaling through direct interaction with Smo, Cyclopamine (10 μM) inhibits the proliferation of SMO^high Cyclopamine-responsive cell lines L3.6sl and Panc 05.04 by 75-80%, and increases the apoptosis by 2.5- to 3.5-fold, without affecting the BxPC3-SMO^low cell line. ^[3] Cyclopamine treatment significantly decreases of Snail mRNA and increasea E-cadherin transcripts in the E3LZ10.7 cell line. Independent of inhibition of cell growth, Cyclopamine treatment significantly inhibits the invasive phenotype of Hedgehog-dependent L3.6pl cells, causing a >500-fold reduction in the number of transmigrating cells, but not that of the Hedgehog-independent cell line Panc-1. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

OS-RC-2	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NID3VGlKSzVyPUWuPFY3PiEQvF2=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DOHH-2	NETYfFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NWTKWWdNUUN3ME25MlM2Pjh7IN88US=>	NFHlZpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
no-10	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NWnMOWNSUUN3ME25MlkxOzlizszN	MmOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LS-513	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NHK0[HBKSzVyPUGxMlM2PDdizszN	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ALL-PO	Mn7PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NWDLW\|ZTUUN3ME2xNU44PzN2IN88US=>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
8-MG-BA	NWi4U2pOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NXnqVpc3UUN3ME2xN{4yOTJ\|IN88US=>	M4T6Z\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
RPMI-8402	NWjQcYRJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Mk\yTWM2OD1zNT64OVM4KM7:TR?=	M3y1XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
EoL-1-cell	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NGm5U2JKSzVyPUG4MlU6PDhizszN	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NALM-6	MkfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M2f6T2lEPTB;MUmuNFE3PyEQvF2=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DEL	NVnqXItLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NWjZWVVGUUN3ME2yNE4yPDdzIN88US=>	M3Kx[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SR	M4nmXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NG[0e5NKSzVyPUKzMlY4OTVizszN	MonuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
697	NYjRbnhTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NUPxNWVlUUN3ME2yOk43OTV3IN88US=>	M4iwTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
COLO-829	M4P1WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NFTJVVBKSzVyPUK2Mlg1QDNizszN	MmXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
EVSA-T	NX;F[nRUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M2nOdWlEPTB;MkeuOVU3OSEQvF2=	Ml3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ATN-1	NHyzVnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MWrJR\|UxRTNzLkKzNlkh\|ryP	M2jo[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
L-363	NVmyXFZQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NGH0TXBKSzVyPUOxMlc1PjFizszN	MmPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LAMA-84	NEnXOnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MUfJR\|UxRTN{LkWyNVEh\|ryP	M1z3OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NOS-1	M1;QU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MVLJR\|UxRTN2LkK5OVYh\|ryP	NHjIWVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
BB30-HNC	MmDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NF7rbJZKSzVyPUO0MlM{ODZizszN	M33SRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BC-1	MknaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M1e5fmlEPTB;M{euPVc1PiEQvF2=	NISyR5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
IST-SL2	NXHQO\|JnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Mnm2TWM2OD1\|OD6yNlQh\|ryP	NF\yPVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
D-392MG	NXHHbldbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Mmj1TWM2OD12MD6yNlE2KM7:TR?=	NHXWW4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
no-11	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NF\ie\|dKSzVyPUSwMlU2OjFizszN	NFHjbVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LC4-1	Mn7hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M{f1VWlEPTB;NECuPFcyPiEQvF2=	NFPQWIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
A388	MlHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NGXicVFKSzVyPUSyMlU5PDhizszN	MojEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NTERA-S-cl-D1	NXuwNGpxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MX;JR\|UxRTR{LkewO\|Qh\|ryP	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CESS	NFzPTG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NHTBRYhKSzVyPUS0MlIzOzJizszN	NITmbZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
RS4-11	NYHsTYJJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NUGwd2pUUUN3ME20PU4xQTN6IN88US=>	M4PL[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MS-1	NGP2T2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NFjoOGtKSzVyPUWwMlk{PTFizszN	NF3PRZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
CTV-1	M{HMXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M1\nTWlEPTB;NUGuNFc1KM7:TR?=	NHXnOpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
D-502MG	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MUjJR\|UxRTVzLk[yO\|Eh\|ryP	NXu0N2ZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ML-2	NUTMdpFsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NFLMVFRKSzVyPUWyMlkyQTVizszN	NGT2WG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
SK-NEP-1	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NFXaVopKSzVyPUWzMlM6OjNizszN	NYjKfYRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LOXIMVI	NVLjS5h4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MWXJR\|UxRTV\|LkW4PFQh\|ryP	M3PJW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DJM-1	M2jBTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NXu2WI0yUUN3ME21Ok4{OzlzIN88US=>	NUTLRphYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
GI-1	NWTOXHNWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NYPT[lA5UUN3ME21Ok43OTR7IN88US=>	MoLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
IST-MES1	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NWr6S4hXUUN3ME22NE42PDl\|IN88US=>	NW\nXJdSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MV-4-11	M2PSbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M4T1PGlEPTB;NkCuOlU{QCEQvF2=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
OVCAR-4	NUfOUpZET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NWPTTWlHUUN3ME22N{42PjV5IN88US=>	NXzmeHN{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KE-37	MonUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NWCxeJFYUUN3ME22Ok4zPjZ6IN88US=>	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
D-542MG	NUXD[ldvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXrJR\|UxRTZ6LkSxN\|Uh\|ryP	NGHFVVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
MHH-PREB-1	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M1q1TGlEPTB;N{KuPFQ1OSEQvF2=	NGDzR249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
MRK-nu-1	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M2Lwe2lEPTB;N{OuOFcxPSEQvF2=	NY\Nd2JXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
D-247MG	NX7NdHJCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NXfEc41qUUN3ME23N{42PDR{IN88US=>	MoPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
OCI-AML2	M3v2VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGDtO\|lKSzVyPUe2Mlk{PjlizszN	M4PhSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LP-1	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NHrKdohKSzVyPUiyMlg4OzFizszN	M4S2clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HCC1599	MkXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NFXvOZFKSzVyPUi0MlI5OzdizszN	MnqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KARPAS-45	MoW0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NYXTbHpRUUN3ME24OE43QTl{IN88US=>	NF7vZXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
BE-13	M1nxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGTQPW1KSzVyPUm5MlA1PzdizszN	NH;RO3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
GCIY	NY\TN\|F1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NVvycIZlUUN3ME25PU4xQTV2IN88US=>	NVf0UHdwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
BV-173	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MUHJR\|UxRTFyMD6zNlUh\|ryP	NIm5UWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LB2518-MEL	M1n4T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MYXJR\|UxRTFyMD63PFkh\|ryP	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KS-1	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWnJR\|UxRTFyMT62N\|kh\|ryP	NVz3c5I4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MOLT-16	M3jqR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M{XvbWlEPTB;MUC0Mlk5PiEQvF2=	NXfjN4M{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1770	Ml62S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MofuTWM2OD1zMEiuO\|g1KM7:TR?=	NIfKfG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NCI-H82	Mo\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M4TBTmlEPTB;MUGwMlk4PiEQvF2=	NFvSRWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NCCIT	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3XRS2lEPTB;MUGyMlUzQSEQvF2=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KALS-1	NV3ncIptT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NFvuTYtKSzVyPUGxOU46PDFizszN	NGjFUJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LB2241-RCC	MoG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NEfvbFZKSzVyPUGxOk43PzlizszN	MoqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HH	NGnV[3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MoHzTWM2OD1zMUeuN\|k2KM7:TR?=	NFXaRm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
HD-MY-Z	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{HOT2lEPTB;MUG4MlQ5QCEQvF2=	NH7nVlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
EB-3	NFL3O4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			Mli2TWM2OD1zMkOuNFk1KM7:TR?=	MkfaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
BL-70	NELHW3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NGPXWZdKSzVyPUGyN{4yOjdizszN	MmjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
K-562	NH3rdIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MYjJR\|UxRTF{Nj6yOFUh\|ryP	NIjiNVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
HT-144	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MoC2TWM2OD1zM{OuNVY1KM7:TR?=	MlXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
PF-382	NGjISI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M1q3TWlEPTB;MUO0MlM3OSEQvF2=	MmH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RPMI-8226	MmG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NVrEdWp[UUN3ME2xN\|UvODR3IN88US=>	M4jNT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-H1355	MoDrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NYrn[4U1UUN3ME2xN\|UvPTh5IN88US=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LXF-289	M3y4ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M2rRcmlEPTB;MUO5Mlc5OSEQvF2=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H69	M1;p[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M161dWlEPTB;MUSyMlk{OiEQvF2=	MoT3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SK-MEL-1	NGji[3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M3PLV2lEPTB;MUS3MlE{KM7:TR?=	Moq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KARPAS-299	MmPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MluyTWM2OD1zNEmuNVIh\|ryP	MlvlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
GB-1	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MY\JR\|UxRTF2OT6zNlIh\|ryP	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CMK	MljlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXLJR\|UxRTF2OT61NVUh\|ryP	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MPP-89	NYH2UWZST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXnJR\|UxRTF3Nj6wN\|Uh\|ryP	NFzRV\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KU812	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MVPJR\|UxRTF4MT65NFIh\|ryP	M{HCWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
REH	MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{PkSGlEPTB;MU[yMlEzPSEQvF2=	MkHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NEC8	NYLTVFVTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MoDETWM2OD1zNkWuNFI3KM7:TR?=	NVPlbol4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KP-N-YS	NYiz[2s2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml64TWM2OD1zNkiuN\|k2KM7:TR?=	NIjTVXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
Ramos-2G6-4C10	NFX0bGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnPZTWM2OD1zNkmuPVE2KM7:TR?=	MnLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
Becker	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M124R2lEPTB;MUe0MlE5KM7:TR?=	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB647-SCLC	MmXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXvJR\|UxRTF5NT64OFUh\|ryP	NYn5VW9tRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LU-139	MlHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M1:2RmlEPTB;MUe4MlAyQSEQvF2=	M2Dzb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
QIMR-WIL	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NH:zW3BKSzVyPUG3PU43PDZizszN	MnnzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NCI-H1395	NUPsXWRUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NHmweJhKSzVyPUG3PU46QTZizszN	NIK3ZpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NOMO-1	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MXPJR\|UxRTF6Mj64OUDPxE1?	NE\WZY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
GI-ME-N	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NEjhO4FKSzVyPUG4O{46PjlizszN	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KMS-12-PE	NHzJfohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVjJR\|UxRTF6OT6yO\|Mh\|ryP	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
Daudi	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{TXUWlEPTB;MUmxMlEzQCEQvF2=	Moe1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LB996-RCC	NHfpbGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVTJR\|UxRTF7MT62PVkh\|ryP	NFHyS\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NCI-H2107	NIOzNVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NITON2pKSzVyPUG5N{44OzlizszN	MnXrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SK-PN-DW	NYW3XG9VT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MV\JR\|UxRTF7ND63NVkh\|ryP	NX;IU4N{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MC-CAR	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NXSzeVVZUUN3ME2yNFIvOjV\|IN88US=>	NFzmdG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
SNB75	M13pSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NXLZWZllUUN3ME2yNlEvQTRizszN	M2L2eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES4	NX3jXItsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NVHtbpBiUUN3ME2yNlMvPzh\|IN88US=>	NUXZ[5IzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KARPAS-422	Ml61S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MUjJR\|UxRTJ{OD6zOVIh\|ryP	NYO0SHZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1648	Mn\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MlnUTWM2OD1{MkmuOFg6KM7:TR?=	MnO4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ES6	NWHzTm1iT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NXHlU3ZQUUN3ME2yN\|kvPDNizszN	NXzvZppCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KNS-81-FD	NHuy[\|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MUHJR\|UxRTJ2MT6xPVch\|ryP	NG\KfWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
JAR	M4rDbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MnPvTWM2OD1{NU[uNlI2KM7:TR?=	NFvSNHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NB1	NHzlSJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NYTzWmtjUUN3ME2yOlAvPTF4IN88US=>	MofyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-336MG	M33h[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NUfhWZc6UUN3ME2yOlAvPjl6IN88US=>	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BC-3	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M1LmemlEPTB;Mk[1MlE4QCEQvF2=	M4SzclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HCC2218	MnfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NF\LdnlKSzVyPUK2Ok41OTVizszN	M1OyelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
TE-9	NX;EU4xtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NYjQR3FWUUN3ME2yOlYvPjJ5IN88US=>	NVXRZm5oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LB1047-RCC	NVe0dI1PT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M370d2lEPTB;Mk[2Mlc2OyEQvF2=	M{jqVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
CTB-1	NXjFc2FKT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NHLVd5FKSzVyPUK2PU46PzNizszN	M1jBbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NB7	NYT0[HNzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MUjJR\|UxRTJ5MTFOwG0>	M4i2cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ST486	NWPNXHlQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NWDUe3ZxUUN3ME2yO\|cvPDF{IN88US=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HCC1187	NH\4ZnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MWLJR\|UxRTJ6Mj64NVEh\|ryP	NFvIWFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NCI-SNU-16	M3PjZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NITpcZFKSzVyPUK4OE4zPDhizszN	M{nmXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
COR-L279	NID0WHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnHvTWM2OD1{OUGuOVg1KM7:TR?=	NFHtUVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
ES8	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3XjemlEPTB;Mkm0MlE5OiEQvF2=	NECzU4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
U-698-M	M17aS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NFTBcXhKSzVyPUK5PE4zPDNizszN	MoCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HEL	MoLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NVTmSm9DUUN3ME2zNFkvOTR7IN88US=>	NYjaOFN[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KINGS-1	MmDJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NWix[m45UUN3ME2zNVAvPjd2IN88US=>	MkTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KY821	M1rGO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MU\JR\|UxRTN\|Nj61PVUh\|ryP	MmfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ1-PC	NGnPNXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnPyTWM2OD1\|NEWuOlE5KM7:TR?=	NIfDdno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LS-411N	M1TMfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NETTdGhKSzVyPUO1OE43PiEQvF2=	Ml7YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SIG-M5	Mk\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M2fFXGlEPTB;M{W5Mlc5OiEQvF2=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HT	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NYLk[pZ1UUN3ME2zOlcvPzFzIN88US=>	MmXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HC-1	M3LER2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			Ml\hTWM2OD1\|NkeuO\|g4KM7:TR?=	NY[0NlN{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1694	M{Pifmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NVTaZ4NXUUN3ME2zO\|IvQTN2IN88US=>	MkjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
BB65-RCC	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NUfXcGozUUN3ME2zO\|YvOjR3IN88US=>	MnfBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HAL-01	NHPmb29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NH\TT3NKSzVyPUO3PU45OzhizszN	NESwXHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
ARH-77	NFTxPWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NWfJdGlIUUN3ME2zPVQvODB6IN88US=>	MoXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ7-mel	NVfiV3lLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M{jBXWlEPTB;M{m3MlI{OyEQvF2=	NGDFRm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
SIMA	NUj5VYVGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MVjJR\|UxRTRyMz65N\|Mh\|ryP	MnPQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
DG-75	NETIRXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MX3JR\|UxRTRzNT62PVgh\|ryP	NX\RfmdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HUTU-80	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MmfoTWM2OD12MUmuNVg2KM7:TR?=	NHz3ZYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KNS-42	MnrDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MYnJR\|UxRTR{NT64NVUh\|ryP	NWTkSYRvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SH-4	NFW4U4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NFLWeZZKSzVyPUSyO{42PjVizszN	M1SwelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
L-540	NFjwb3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M37hcWlEPTB;NEOxMlA{OSEQvF2=	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB10	NG\OcolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M1m3XGlEPTB;NESxMlI{PCEQvF2=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES1	NX;HdHZIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml3rTWM2OD12NUKuO\|U{KM7:TR?=	NVLNRZVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KMOE-2	MlHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NHnUPGtKSzVyPUS1Ok44OTFizszN	M3PWfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MC116	MoDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MnmzTWM2OD12NUiuNVE3KM7:TR?=	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RCC10RGB	NE\Y[ohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MoT0TWM2OD12NkCuNFA2KM7:TR?=	NGHSdZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
RL95-2	MlLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NUjqdGN4UUN3ME20OlAvOjN5IN88US=>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
Raji	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NIfGcJJKSzVyPUS2PE4yPDNizszN	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CAS-1	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MkK5TWM2OD12N{KuNFc{KM7:TR?=	NVfsR2JkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
Calu-6	NYTsbZc3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NETVc5hKSzVyPUS3OU4zPjVizszN	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KG-1	M3Lpc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NW\VN3doUUN3ME20O\|gvPDRizszN	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB771-HNC	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWjJR\|UxRTR6Mj6yN\|Ih\|ryP	Mlf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ACN	NGTMem9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnjZTWM2OD12OUOuOVk6KM7:TR?=	M2n2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KM12	NGLWZZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NH7SWHNKSzVyPUS5Ok42QDlizszN	M4DqdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
U2OS	MV\GeY5kfGmxbjDhd5NigQ>?			MoHrRolv\GmwZzDh[oZqdmm2eTD0c{B4cWymIIT5dIUhW22xIHX4dJJme3OnZDDpckBWOk:VIHPlcIx{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEumIE2gNE4xOTJ2IN88UU4>	M4r0ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[zOVIzLz5{M{C2N\|UzOjxxYU6=
U2OS	NIrZ[mpHfW6ldHnvckBie3OjeR?=		MX6yJIhzew>?	NEHaWpZFcXOybHHj[Y1mdnRib3[gX\|NJZWO7Y3zvdIFucW6nIH\yc40hf2muZDD0fZBmKFOvbzDlfJBz\XO\|ZXSgbY4hXTKRUzDj[YxteyCjZoTldkAzKGi{czDifUB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbUA:KDBwMEGyO{DPxE1w	NGTyZm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2N\|UzOid-MkOwOlM2OjJ:L3G+
TM3	NHrP[IFHfW6ldHnvckBie3OjeR?=		NHiwTHQ1QCCqcoO=	M3fyeGlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViVF2zJINmdGy\|IHLlZZJqdmdicGTBMVh5T2yrLVz1Z{Bz\XCxcoTldkBkd26\|dIL1Z5Qh[XO\|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKG2xZIXsZZRqd25ib3[gS4xqKGGodHXyJFQ5KGi{czDifUBtfWOrZnXyZZNmKGG\|c3H5MEBKSzVyIE2gNE4xPDZizszNMi=>	NEPmS409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC5NVU2QSd-MUmwPVE2PTl:L3G+
HCC827	MVrGeY5kfGmxbjDhd5NigQ>?			Ml7FSIl{eGyjY3Xt[Y51KG:oIGuzTH0u[3mlbH;wZY1qdmViZoLvcUBUVU9iVkSwOG0hdXW2YX70JIlvKGenZnn0bY5q[iC{ZYPpd5RidnRiaIXtZY4hUEOFOEK3JINmdGy\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFvpJF0hOC5yNUGg{txONg>?	MkX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OEexOVYoRjJ6N{i3NVU3RC:jPh?=
HEK293	NWSyVWJmTnWwY4Tpc44h[XO\|YYm=		MYOyJIhzew>?	MYLEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWyjYnXscIVlKGO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNkBpenNiYomg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHluIFnDOVAhRSByLkC2OEDPxE1w	Mli0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Nki1OVEoRjJ{Mk[4OVUyRC:jPh?=
Shh Light2	NXHBPHBOTnWwY4Tpc44h[XO\|YYm=		NWS5XJdXOzBiaILz	NX7oT2UzUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W\|ZTDTbIghVGmpaISyJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2Zic3;ubYMhcGWmZ3Xoc4cucW6mdXPl[EBIVElzLX3l[IlifGWmIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCvZXHzeZJm\CCjZoTldkA{OCCqcoOgZpkh\HWjbDDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjB5NEGg{txONg>?	M4LVNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NU[3N\|cyLz5{N{W2O\|M4OTxxYU6=
U2OS	NVzRSI5ETnWwY4Tpc44h[XO\|YYm=			NYDZbFVsSmmwZHnu[{Bi\m[rbnn0fUB1dyCVbX:gSFQ4O0hibYX0ZY51KGW6cILld5Nm\CCrbjDVNm9UKGOnbHzzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtlKD1iMD6xNVYh\|ryPLh?=	NIL1UYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2N\|UzOid-MkOwOlM2OjJ:L3G+
DaOY	M1:5SGZ2dmO2aX;uJIF{e2G7		NXPUPFNvPDhiaILz	Mm\kTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC\|aXfuZYxqdmdiaX6gbJVu[W5iRHHPXUBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kBIdGlzIH3SUmEh\XiycnXzd4lwdiCjZoTldkA1QCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nzMEBKSzVyIE2gNE4yPiEQvF2u	M1HQc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO\|E3Lz5{NEmwNFcyPjxxYU6=
U2OS	MkXGSpVv[3Srb36gZZN{[Xl?		Mk\CNkBpenN?	M3\Cd2Rqe3CuYXPlcYVvfCCxZjDbN2he[3mlbH;wZY1qdmViZoLvcUBUdW9iRES3N2ghdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHHmeIVzKDJiaILzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqKD1iMD6yN\|Ih\|ryPLh?=	Ml;NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ\|ME[zOVIzRC:jPh?=
CHO	NGC0ToxHfW6ldHnvckBie3OjeR?=			M{C4O2FvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hW22xIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[nliW{PIYWhpNUGpIHLpcoRqdmdiYYPzZZktKEmFNUCgQUAxNjJ6IN88UU4>	M2TLTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmxOVU6Lz5zOUC5NVU2QTxxYU6=
Shh Light2	NH3ad4FHfW6ldHnvckBie3OjeR?=		MUO0NEBpenN?	MULJcohq[mm2aX;uJI9nKFOKSDDwZZRpf2G7IHnuJI1wfXOnIGPobEBNcWeqdEKgZ4VtdHNiYX\0[ZIhPDBiaILzJIJ6KEeuaT3k[ZBmdmSnboSgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5\|IN88UU4>	NFPxWFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW5Nlc5QCd-MkG1PVI4QDh:L3G+
Shh-light2	M2nJO2Z2dmO2aX;uJIF{e2G7			NHHBRmRKdmirYnn0bY9vKG:oIGPtc{1u\WSrYYTl[EBJcCC\|aXfuZYxqdmdiaX6gbJVu[W5iU3joMYxq\2i2MjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjNizszNMi=>	MnTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Nki1OVEoRjJ{Mk[4OVUyRC:jPh?=
M210B4	MnrQSpVv[3Srb36gZZN{[Xl?		MWWyOEBpenN?	MnrDTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC\|aXfuZYxqdmdiaX6gcY92e2ViTUKxNGI1KGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKFC2Y3igcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjR\|IN88UU4>	NW\ybJppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4OTZpPkK0PVAxPzF4PD;hQi=>
Shh-Light 2	MlXaSpVv[3Srb36gZZN{[Xl?		MViyJIRigXN?	NF\CT4dCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IGPtc{BqdiCvb4Xz[UBUcGhvTHnnbJQhOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHNpcC2rbnT1Z4VlKEeuaUGtdoVxd3K2ZYKgZYN1cX[rdImgZYZ1\XJiMjDkZZl{KGK7IHT1ZYwudHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJI1mfGixZDygTWM2OCB;IECuOFg1KM7:TT6=	NV[5eIxqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlM2OjJpPkKzNFY{PTJ{PD;hQi=>
Shh Light2	MknTSpVv[3Srb36gZZN{[Xl?			Mof4TY5pcWKrdHnvckBw\iCVSFigbY4hdW:3c3WgV4hpKEyrZ3j0NkBk\WyuczDifUBIVElvcnXzdI9ve2m4ZTDmbZJm\my7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvPSEQvF2u	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTNyOUC4NEc,OTl\|MEmwPFA9N2F-
C3H10T1/2	NXr3N3p3TnWwY4Tpc44h[XO\|YYm=			Ml3nTY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIGPITEBi[3SrdnH0[YQheGG2aIfhfUBqdiCvb4Xz[UBEO0hzMGSxM\|Ih[2WubIOgZZN{\XO\|ZXSgZZMhW0GJLXnu[JVk\WRiY3XscEBlcW[oZYLlcpRq[XSrb36gZpkh[WytYXzpcoUheGixc4DoZZRie2ViYYPzZZktKEmFNUCgQUAxNjZizszNMi=>	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV7Mke4PEc,OjF3OUK3PFg9N2F-
C3H10T1/2	NHHSOFhHfW6ldHnvckBie3OjeR?=		MlK3OkBpenN?	NFjNRVJKdmirYnn0bY9vKG:oIGPBS{1qdmS3Y3XkJIRq\m[ncnXueIlifGmxbjDv[kBud3W\|ZTDt[ZNmdmOqeX3hcEBxdHW{aYDveIVvfCCFM1ixNHQyNzJiY3XscJMhfG9iYXzrZYxqdmVicHjvd5Bp[XSjc3WgdI9{cXSrdnWgc4V{\W:kbHHzeJMh[W[2ZYKgOkBpenNuIFnDOVAhRSByLk[yJO69VS5?	MlLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Nki1OVEoRjJ{Mk[4OVUyRC:jPh?=
ASZ001	M1LmPWZ2dmO2aX;uJIF{e2G7		MkTnOFghcHK\|	NGLSUnJKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W\|ZTDBV3oxODFiY3XscJMh[XO\|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiR3zpNUBuWk6DIHX4dJJme3Orb36gZYZ1\XJiNEigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyxiSVO1NEA:KDBwNk[g{txONg>?	NVHCbVQ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4OTZpPkK0PVAxPzF4PD;hQi=>
M210B4	MUDGeY5kfGmxbjDhd5NigQ>?		M1jHO\|I1KGi{cx?=	MonjTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC\|aXfuZYxqdmdiaX6gcY92e2ViTUKxNGI1KGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjhizszNMi=>	MnXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO\|E3RC:jPh?=
medulloblastoma cells	Mn\rRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			NUDWRXp{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W\|ZTDt[YR2dGyxYnzhd5RwdWFiY3XscJMhcGG{Yn;ybY5oKGindHXyc5p6\2:3czDweINpOSCpZX7lJIJ6KE2WVDDhd5NigSxiRVO1NEA:KDFizszNMi=>	NYrKW2g2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
CHO	NHGyVVFHfW6ldHnvckBie3OjeR?=			NVXOTYdNSW62YXfvcol{fCCjY4Tpeol1gSCjdDDtc5V{\SCVbX:gdoVk\XC2b4Kg[ZhxemW\|c3XkJIlvKEOKTzDj[YxteyCkeTDbN2heUGhvQXegZolv\GmwZzDhd5NigSxiSVO1NEA:KDFwMjFOwG0v	M2XNRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmxOVU6Lz5zOUC5NVU2QTxxYU6=
Shh-Light2	M2LnUGZ2dmO2aX;uJIF{e2G7		NFH2[481QCCqcoO=	MoPOTY5pcWKrdHnvckBw\iCVSFigbY4hdW:3c3WgV4hpNUyrZ3j0NkBk\WyuczDh[pRmeiB2ODDodpMh[nliR3zpNUBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJHNCTyxiSVO1NEA:KDFwM{GyJO69VS5?	MnvBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3NEG0PVAoRjF7NUSxOFkxRC:jPh?=
MEF	NHPG[XVHfW6ldHnvckBie3OjeR?=			MoDkTY5pcWKrdHnvckBw\iCVbX:gbY4hdW:3c3WgVJRkcC2mZX\pZ4lmdnRiTVXGJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiU3joMYlv\HWlZXSgS4xqOSC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJktKEmFNUCgQUAyNjVizszNMi=>	NFHpfmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3OFU1OSd-MkOwO\|Q2PDF:L3G+
MEF	MW\GeY5kfGmxbjDhd5NigQ>?		MUGzNEBpenN?	MW\Jcohq[mm2aX;uJI9nKFOvbzDpckBud3W\|ZTDQeINpNWSnZnnjbYVvfCCPRV[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCVaHitbY5lfWOnZDDHcIkuemW\|cH;ud4l3\SCkZYTh[4Ft[WO2b4Pp[IF{\SCjY4Tpeol1gSCjZoTldkA{OCCqcoOgZpkhSmW2YVfMc{Bie3OjeTygTWM2OCB;IEGuPUDPxE1w	NX7EflVoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO\|Q2PDFpPkKzNFc1PTRzPD;hQi=>
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HEK293	NFWwN2JHfW6ldHnvckBie3OjeR?=			M1O2WmlvcGmkaYTpc44hd2ZiYnX0ZUBo[WyjY4Tvd4ll[XOnIHnuJGhGUzJ7MzDj[Yxtew>?	MoK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OUS3OlYoRjF5NEm0O\|Y3RC:jPh?=
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C3H10T1/2	MU\GeY5kfGmxbjDhd5NigQ>?	MYm1OU42KHSxIES1NFAhdk1?		NXzCSXQ5S2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBUdW9iaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhW0GJLXnu[JVk\WRiR3zpNUB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZZQhPTVwNTD0c{A1PTByIH7NJIJ6KHGSQ2KgZY5idHm\|aYO=	MkGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyN{S1OFEoRjJ\|MEe0OVQyRC:jPh?=
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Snail / E-cadherin / Slug / Vimentin ; Gli1 / TGF-β1 / CXCR4 ; NF-κB / Cyclin D1 / MMP2 / MMP9	26859575 26137001 23599805
Immunofluorescence	PCNA / β-catenin ; Vimentin	28747625 24553082

In vivo

Administration of Cyclopamine at dose of 50 mg/kg/day for 22 days eradicates the HUCCT1 xenografts in mice with no obvious adverse effects. ^[2] Cyclopamine treatment at dose of 1.2 mg for 7 days induces significant apoptosis of tumor cells and decreases the tumor mass by 50-60% in Panc 05.04- and L3.6sl-derived tumors, respectively, but not in the BxPC3-SMO^low tumors. [3] Administration of Cyclopamine alone profoundly inhibits tumor metastases in xenografts of E3LZ10.7 and L3.6pl, and completely abrogates metastases when in combination of gemcitabine. ^[4]

Protocol (from reference)

Kinase Assay:^[1]	Hedgehog cell assay: This assay measures the end stage of the Hh signaling pathway, that is, the transcriptional modulation of Gli, using Luciferase as readout (Gli-Luc assay). Cyclopamine is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are resuspended in F12 Ham's/DMEM (1:1) containing 5% FBS and 15 mM Hepes pH 7.3, added to assay plates and incubated with Cyclopamine for approximately 30 minutes at 37 °C in 5% CO₂. 1 nM Hh-Ag 1.5 is then added to assay plates and incubated at 37 °C in the presence of 5% CO₂. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 value, defined as the inflection point of the logistic curve, is determined by non-linear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of Cyclopamine using the R statistical software pack
Cell Research:^[2]	Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, and HCT116 Concentrations: Dissolved in DMSO, final concentration 3 μM Incubation Time: 4 days Method: Cells are exposed to Cyclopamine in 96-well plates. Cell viability is measured by MTS (soluble tetrazolium salt) assay. Viable cell mass is determined by optical density measurements at 490 nm (OD₄₉₀) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)﹣OD (day 2)/OD (day 2). (Only for Reference)
Animal Research:^[2]	Animal Models: Athymic (nude) mice inoculated subcutaneously with HUCCT1 cells Dosages: 50 mg/kg/day Administration: Subcutaneous injection (Only for Reference)

References

[4] Feldmann G, et al. Cancer Res, 2007, 67(5), 2187-2196.

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Solubility (25°C)

In vitro	DMF	10 mg/mL warmed (24.29 mM)
	Ethanol	2 mg/mL warmed (4.85 mM)
	DMSO	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	411.62
Formula	C₂₇H₄₁NO₂
CAS No.	4449-51-8
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5CC4=C3C)C)O)C)NC1

Download Cyclopamine SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo use in mice?

Answer:
One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

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