Cyclopamine

For research use only.

Catalog No.S1146 Synonyms: 11-deoxojervine

79 publications

Cyclopamine Chemical Structure

CAS No. 4449-51-8

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

Size Price Stock Quantity  
USD 147 In stock
USD 270 In stock
USD 370 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Cyclopamine has been cited by 79 publications

Purity & Quality Control

Choose Selective Hedgehog/Smoothened Inhibitors

Biological Activity

Description Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.
Targets
Smoothened [1]
(TM3Hh12 cells)
46 nM
In vitro

Cyclopamine inhibits the Hedgehog signaling pathway with an IC50 of 46 nM, and blocks the activity of human Smo receptor expressed in CHO-K1 cells in [3H]Hh-Ag binding assay with an IC50 of 280 nM. [1] Cyclopamine significantly inhibits Hedgehog pathway activity in a dose-dependent manner in gut-derived tumor cell lines expressing Patched (PTCH) mRNA, and induces growth inhibition of those tumor cell lines by 75-95% at the concentration of 3 μM, but ineffective towards the colon tumor cells without PTCH mRNA expression, suggesting the effects of Cyclopamine treatment are Hedgehog pathway related rather than generally cytotoxic. [2] By blocking Hedgehog signaling through direct interaction with Smo, Cyclopamine (10 μM) inhibits the proliferation of SMOhigh Cyclopamine-responsive cell lines L3.6sl and Panc 05.04 by 75-80%, and increases the apoptosis by 2.5- to 3.5-fold, without affecting the BxPC3-SMOlow cell line. [3] Cyclopamine treatment significantly decreases of Snail mRNA and increasea E-cadherin transcripts in the E3LZ10.7 cell line. Independent of inhibition of cell growth, Cyclopamine treatment significantly inhibits the invasive phenotype of Hedgehog-dependent L3.6pl cells, causing a >500-fold reduction in the number of transmigrating cells, but not that of the Hedgehog-independent cell line Panc-1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
OS-RC-2 M1fEZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLySYtKSzVyPUWuPFY3PiEQvF2= NEDacmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
DOHH-2 NX\yR4szT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIj3T4tKSzVyPUmuN|U3QDlizszN Mnn1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
no-10 NYT5bG9XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV2xfm5YUUN3ME25MlkxOzlizszN NWnOPHpWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LS-513 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljxTWM2OD1zMT6zOVQ4KM7:TR?= MmTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ALL-PO NGPKcI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M360[2lEPTB;MUGuO|c{PCEQvF2= MomxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
8-MG-BA M2TPd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfRTWM2OD1zMz6xNVI{KM7:TR?= NILsZXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
RPMI-8402 MlntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILoUpZKSzVyPUG1Mlg2OzdizszN NGe1W2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
EoL-1-cell M1rRPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDXTWM2OD1zOD61PVQ5KM7:TR?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NALM-6 NHHNSZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTF7LkCxOlch|ryP NWKwXnF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
DEL NHfo[|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTJyLkG0O|Eh|ryP NF3pbGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
SR MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTJ|Lk[3NVUh|ryP Mn[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
697 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDtPGUxUUN3ME2yOk43OTV3IN88US=> M3XFOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
COLO-829 NVTJOJhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDWUXpKSzVyPUK2Mlg1QDNizszN M1fUdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
EVSA-T NF75TIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWO4SFd4UUN3ME2yO{42PTZzIN88US=> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ATN-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvHZ2VKSzVyPUOxMlI{OjlizszN M1nvSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
L-363 NEDzNXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PsUGlEPTB;M{GuO|Q3OSEQvF2= M4D6bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LAMA-84 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoO3TWM2OD1|Mj61NlEyKM7:TR?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NOS-1 NVPKcGhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfQSG5KSzVyPUO0MlI6PTZizszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BB30-HNC MkDwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTN2LkOzNFYh|ryP NGrmTm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
BC-1 M3HSWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVf6W49HUUN3ME2zO{46PzR4IN88US=> NI\GcVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
IST-SL2 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPhRXJKSzVyPUO4MlIzPCEQvF2= NVn5T2NNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
D-392MG NEj5RnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYr1NYx3UUN3ME20NE4zOjF3IN88US=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
no-11 MmrLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33KfGlEPTB;NECuOVUzOSEQvF2= Mn3XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LC4-1 NHTjNGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTRyLki3NVYh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
A388 NYX0UZZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLyTWM2OD12Mj61PFQ5KM7:TR?= MlfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NTERA-S-cl-D1 NEDK[5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjWPHZTUUN3ME20Nk44ODd2IN88US=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CESS NIqySZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHqzZVNKSzVyPUS0MlIzOzJizszN MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RS4-11 M4XrXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVG1NpJNUUN3ME20PU4xQTN6IN88US=> MljvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MS-1 M2Wyb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn32TWM2OD13MD65N|UyKM7:TR?= MmXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CTV-1 M3rDUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\ucWlEPTB;NUGuNFc1KM7:TR?= Mki0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-502MG NWS2N|Q{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDGUXZKSzVyPUWxMlYzPzFizszN NX7BVW5ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ML-2 NWPpPHZET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUC4OlR7UUN3ME21Nk46OTl3IN88US=> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
SK-NEP-1 MoHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\OfmlEPTB;NUOuN|kzOyEQvF2= NVe2cIxtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LOXIMVI NH[1c45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnTWIJKSzVyPUWzMlU5QDRizszN MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DJM-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;TV2lEPTB;NU[uN|M6OSEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
GI-1 NHThbplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYD5bHpuUUN3ME21Ok43OTR7IN88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
IST-MES1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3LTWM2OD14MD61OFk{KM7:TR?= NFHXb5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
MV-4-11 NYLPNGNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1S1UWlEPTB;NkCuOlU{QCEQvF2= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
OVCAR-4 NXXXT4Z5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTZ|LkW2OVch|ryP M{LMdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KE-37 NUDJNHhpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;2TWM2OD14Nj6yOlY5KM7:TR?= NYLz[HRvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
D-542MG MoL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1flXGlEPTB;NkiuOFE{PSEQvF2= NYPGSo93RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MHH-PREB-1 NXLmSWNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTMTWM2OD15Mj64OFQyKM7:TR?= M3PKV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MRK-nu-1 NGXV[FVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHu5NnlKSzVyPUezMlQ4ODVizszN NUnwO4NuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
D-247MG NHHGbpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTd|LkW0OFIh|ryP M2n4VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
OCI-AML2 MojOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoC4TWM2OD15Nj65N|Y6KM7:TR?= NFXJe2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
LP-1 Mn3PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXPUJJKSzVyPUiyMlg4OzFizszN Ml23QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HCC1599 Mmf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTh2LkK4N|ch|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KARPAS-45 NVvVSVI5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfUZWFKSzVyPUi0MlY6QTJizszN M2HmWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BE-13 NHTmXZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nUb2lEPTB;OUmuNFQ4PyEQvF2= M3fs[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
GCIY M2nGOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nmSGlEPTB;OUmuNFk2PCEQvF2= M3u5U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BV-173 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHCTWM2OD1zMECuN|I2KM7:TR?= M3;BPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LB2518-MEL Mo\TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoO3TWM2OD1zMECuO|g6KM7:TR?= NFj3S449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KS-1 MmfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILkRY5KSzVyPUGwNU43OzlizszN NEP5WlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
MOLT-16 NGDFW|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPvOHFZUUN3ME2xNFQvQTh4IN88US=> NIWyNYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NCI-H1770 NYXVOllkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXnNZhMUUN3ME2xNFgvPzh2IN88US=> NH3FcnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NCI-H82 NGe0UmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTFzMD65O|Yh|ryP NULJeGRGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCCIT NFThRlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTzZlVWUUN3ME2xNVIvPTJ7IN88US=> M4fWcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KALS-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7kc2NKUUN3ME2xNVUvQTRzIN88US=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB2241-RCC MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfjTWM2OD1zMU[uOlc6KM7:TR?= NYnSWXZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HH MkLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jhNWlEPTB;MUG3MlM6PSEQvF2= NH:xNpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HD-MY-Z NGmxeY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnUdHR6UUN3ME2xNVgvPDh6IN88US=> Mk\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
EB-3 NFeyfZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIf2R2RKSzVyPUGyN{4xQTRizszN Mn;HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
BL-70 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;BR5BKSzVyPUGyN{4yOjdizszN NXTJS4hNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
K-562 M{PaOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlz1TWM2OD1zMk[uNlQ2KM7:TR?= M{HZcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HT-144 M{HTb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTF|Mz6xOlQh|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
PF-382 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTF|ND6zOlEh|ryP NHzucG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
RPMI-8226 MnnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTwTWM2OD1zM{WuNFQ2KM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1355 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIP5fYJKSzVyPUGzOU42QDdizszN M2XudVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LXF-289 MkX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33jWmlEPTB;MUO5Mlc5OSEQvF2= NWP3eWFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H69 M4KwPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTF2Mj65N|Ih|ryP MmrPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SK-MEL-1 NWLmWot5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3wXWRKSzVyPUG0O{4yOyEQvF2= NUDyfHYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KARPAS-299 Ml3JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTF2OT6xNkDPxE1? NGe5Om09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
GB-1 NXnxSmR{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHv6V2VKSzVyPUG0PU4{OjJizszN NF;ofJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
CMK MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYK2do5RUUN3ME2xOFkvPTF3IN88US=> NUjHNY1TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MPP-89 NYXnbFdxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TOO2lEPTB;MUW2MlA{PSEQvF2= Mm\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KU812 NVXUXHg3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;TOYk6UUN3ME2xOlEvQTB{IN88US=> M4LNRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
REH NWPId5plT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{j0eWlEPTB;MU[yMlEzPSEQvF2= M1f2O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NEC8 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PVS2lEPTB;MU[1MlAzPiEQvF2= NXXIWVlnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KP-N-YS Mkj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULxd|d2UUN3ME2xOlgvOzl3IN88US=> MmLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
Ramos-2G6-4C10 Ml75S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTRb5JFUUN3ME2xOlkvQTF3IN88US=> NHvzTXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
Becker MoLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPQeGdsUUN3ME2xO|QvOThizszN MkDnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LB647-SCLC NHHadVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17BZWlEPTB;MUe1Mlg1PSEQvF2= NWPPW3lHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LU-139 NEPTUZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NISwTo9KSzVyPUG3PE4xOTlizszN MlG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
QIMR-WIL NECweJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XHUWlEPTB;MUe5MlY1PiEQvF2= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1395 NUjUb257T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLJTWM2OD1zN{muPVk3KM7:TR?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NOMO-1 NW\IZm9VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknHTWM2OD1zOEKuPFUh|ryP M1fu[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
GI-ME-N NEfqZlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfRVVNOUUN3ME2xPFcvQTZ7IN88US=> M3nx[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KMS-12-PE NFn3NlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTF6OT6yO|Mh|ryP NEfZOHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
Daudi MknKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGrUU5hKSzVyPUG5NU4yOjhizszN M1XYNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LB996-RCC NWn3SFM3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTodmZGUUN3ME2xPVEvPjl7IN88US=> MlfJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NCI-H2107 NFzFSlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTF7Mz63N|kh|ryP NVTac4VrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SK-PN-DW MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPWR2lKSzVyPUG5OE44OTlizszN NXX5bXZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MC-CAR MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{iyVWlEPTB;MkCyMlI2OyEQvF2= M1n5XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SNB75 M4jhemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTJ{MT65OEDPxE1? MoHpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ES4 M1nZeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTJ{Mz63PFMh|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KARPAS-422 NHi5cldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITMVY5KSzVyPUKyPE4{PTJizszN MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1648 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTJ{OT60PFkh|ryP M{CwW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES6 M{jtSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTJ|OT60N{DPxE1? NH[yeXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KNS-81-FD NH3QdW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknSTWM2OD1{NEGuNVk4KM7:TR?= NHTtUok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
JAR Mo\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzLTWM2OD1{NU[uNlI2KM7:TR?= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVq5em5KUUN3ME2yOlAvPTF4IN88US=> MmXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-336MG Ml3OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nFbWlEPTB;Mk[wMlY6QCEQvF2= NU\yOmJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
BC-3 NX\IWZR3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvSTWM2OD1{NkWuNVc5KM7:TR?= M3PkcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HCC2218 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPHTWM2OD1{Nk[uOFE2KM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
TE-9 NUHNUmE2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmq1TWM2OD1{Nk[uOlI4KM7:TR?= NVv2PZppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LB1047-RCC NYL3d|RpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfKfIZKSzVyPUK2Ok44PTNizszN NF;1Vow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
CTB-1 M4S0bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3:yXGlEPTB;Mk[5Mlk4OyEQvF2= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB7 MlHKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTJ5MTFOwG0> NFO0PJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ST486 M1vPVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjWTHd5UUN3ME2yO|cvPDF{IN88US=> NFjUOXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HCC1187 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LS[mlEPTB;MkiyMlgyOSEQvF2= M3zSNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-SNU-16 NFH6NZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjDTWM2OD1{OESuNlQ5KM7:TR?= M1K2WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
COR-L279 NYLKW|BZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{D1XGlEPTB;MkmxMlU5PCEQvF2= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES8 NYH1eFhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTJ7ND6xPFIh|ryP Mmq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
U-698-M NYXVTpQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfMOJJKSzVyPUK5PE4zPDNizszN NY\EdmxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HEL NIjvc4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXETWM2OD1|MEmuNVQ6KM7:TR?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KINGS-1 Mk\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTNzMD62O|Qh|ryP NIPyT2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KY821 NH;0R2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTN|Nj61PVUh|ryP M3jCOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MZ1-PC M{jB[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml74TWM2OD1|NEWuOlE5KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LS-411N NHrwSoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfPTWM2OD1|NUSuOlYh|ryP Mnn4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SIG-M5 Moe5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\jXWlEPTB;M{W5Mlc5OiEQvF2= NVfIVW9SRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HT NHWyXYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjwfY5KSzVyPUO2O{44OTFizszN MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HC-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\U[G9IUUN3ME2zOlcvPzh5IN88US=> NUKzfFk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1694 NXW0TodvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTN5Mj65N|Qh|ryP NXLDN4dRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
BB65-RCC NIj1PZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;vOIZXUUN3ME2zO|YvOjR3IN88US=> M2K4ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HAL-01 M4j2cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3qxXmlEPTB;M{e5Mlg{QCEQvF2= NXnpRWpmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ARH-77 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\DNWlEPTB;M{m0MlAxQCEQvF2= MlH0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ7-mel MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljuTWM2OD1|OUeuNlM{KM7:TR?= NYPCW5NVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SIMA M3LuN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTRyMz65N|Mh|ryP NY\ZZodtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
DG-75 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXT[FBrUUN3ME20NVUvPjl6IN88US=> M3TkUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HUTU-80 MmDMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTRzOT6xPFUh|ryP NULnU41CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KNS-42 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTR{NT64NVUh|ryP M3vle|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SH-4 NWDEVZhIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHuzNpRKSzVyPUSyO{42PjVizszN NGO4V489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
L-540 NXqzO2NxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTR|MT6wN|Eh|ryP NYrnfow5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NB10 Ml3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXrU4xKSzVyPUS0NU4zOzRizszN NFr0SnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ES1 Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTR3Mj63OVMh|ryP NFi4dFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KMOE-2 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUT2V2hUUUN3ME20OVYvPzFzIN88US=> NFLsbJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
MC116 NYizdGtPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrEOmUxUUN3ME20OVgvOTF4IN88US=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RCC10RGB NUnZRXJFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml6zTWM2OD12NkCuNFA2KM7:TR?= M3\pWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
RL95-2 M3;QT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzmW5hKSzVyPUS2NE4zOzdizszN NEHz[|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
Raji Mk\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTR4OD6xOFMh|ryP NHfXb409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
CAS-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1H4[GlEPTB;NEeyMlA4OyEQvF2= NWrQUFJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
Calu-6 MnjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTR5NT6yOlUh|ryP NWG4XXdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KG-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrERo15UUN3ME20O|gvPDRizszN MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB771-HNC MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTR6Mj6yN|Ih|ryP NHnhbVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ACN NFXMXIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGr1VI5KSzVyPUS5N{42QTlizszN NV:0epFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KM12 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTR7Nj61PFkh|ryP MnH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
U2OS MlPqSpVv[3Srb36gZZN{[Xl? NFK5TW5DcW6maX7nJIFn\mmwaYT5JJRwKHerbHSgeJlx\SCVbX:g[ZhxemW|c3XkJIlvKFV{T2OgZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3SgQUAxNjBzMkSg{txONg>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
U2OS MnTQSpVv[3Srb36gZZN{[Xl? M4Owe|IhcHK| NWXzc5lKTGm|cHzhZ4Vu\W62IH;mJHs{UF2leXPsc5BidWmwZTDmdo9uKHerbHSgeJlx\SCVbX:g[ZhxemW|c3XkJIlvKFV{T2OgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSByLkCxNlch|ryPLh?= NVjPUXJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlM2OjJpPkKzNFY{PTJ{PD;hQi=>
TM3 M1TqTmZ2dmO2aX;uJIF{e2G7 M2jJcVQ5KGi{cx?= NIjUZWJKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIGTNN{Bk\WyuczDi[YFzcW6pIIDURU05gEeuaT3MeYMhemWyb4L0[ZIh[2:wc4TyeYN1KGG|c3Xzd4VlKGG|IITyZY5{[3KrcITpc45idCCvb3T1cIF1cW:wIH;mJGdtcSCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SCjc4PhfUwhUUN3MDC9JFAvODR4IN88UU4> NFvj[WU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC5NVU2QSd-MUmwPVE2PTl:L3G+
HCC827 MoHRSpVv[3Srb36gZZN{[Xl? MYDEbZNxdGGlZX3lcpQhd2ZiW{PIYU1kgWOub4DhcYlv\SCocn;tJHNOVyCYNEC0UUBufXSjboSgbY4h\2WoaYTpcoljKHKnc3nzeIFvfCCqdX3hckBJS0N6MkegZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3mgQUAxNjB3MTFOwG0v Mor3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OEexOVYoRjJ6N{i3NVU3RC:jPh?=
HEK293 NGP5UXRHfW6ldHnvckBie3OjeR?= MmHONkBpenN? MXLEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWyjYnXscIVlKGO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNkBpenNiYomg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHluIFnDOVAhRSByLkC2OEDPxE1w M2fBWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[4OVUyLz5{MkK2PFU2OTxxYU6=
Shh Light2 MnXmSpVv[3Srb36gZZN{[Xl? NFyxWpk{OCCqcoO= M3j6bmlvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViU3joJGxq\2i2MjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHOxbnnjJIhm\GenaH;nMYlv\HWlZXSgS2xKOS2vZXTpZZRm\CC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkhdWWjc4Xy[YQh[W[2ZYKgN|AhcHK|IHL5JIR2[WxibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4xPzRzIN88UU4> MmHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3NkezO|EoRjJ5NU[3N|cyRC:jPh?=
U2OS NIjyNmNHfW6ldHnvckBie3OjeR?= MYjCbY5lcW6pIHHm[olvcXS7IITvJHNudyCGNEezTEBufXSjboSg[ZhxemW|c3XkJIlvKFV{T2OgZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3SgQUAxNjFzNjFOwG0v NF7RW3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2N|UzOid-MkOwOlM2OjJ:L3G+
DaOY MW\GeY5kfGmxbjDhd5NigQ>? M{[zdFQ5KGi{cx?= NET1RllKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBpfW2jbjDEZW9[KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjF4IN88UU4> M4LqfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO|E3Lz5{NEmwNFcyPjxxYU6=
U2OS MUTGeY5kfGmxbjDhd5NigQ>? M3vsRVIhcHK| NEj1[mdFcXOybHHj[Y1mdnRib3[gX|NJZWO7Y3zvdIFucW6nIH\yc40hW22xIFS0O|NJKG23dHHueEBmgHC{ZYPz[YQhcW5iVULPV{Bk\WyuczDh[pRmeiB{IHjyd{BjgSC|Y3nueIltdGG2aX;uJINwfW62aX7nMEBMcSB;IECuNlMzKM7:TT6= MknCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ|ME[zOVIzRC:jPh?=
CHO MnPUSpVv[3Srb36gZZN{[Xl? NX35SYlySW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCVbX:gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyCkeTDbN2heUGhvQXegZolv\GmwZzDhd5NigSxiSVO1NEA:KDBwMkig{txONg>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB7MUW1PUc,OTlyOUG1OVk9N2F-
Shh Light2 NEDqTG9HfW6ldHnvckBie3OjeR?= NXTzTnF4PDBiaILz NHjPVFBKdmirYnn0bY9vKG:oIGPITEBx[XSqd3H5JIlvKG2xdYPlJHNpcCCOaXfoeFIh[2WubIOgZYZ1\XJiNECgbJJ{KGK7IFfsbU1l\XCnbnTlcpQhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxKD1iMD6zJO69VS5? NGHMfYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW5Nlc5QCd-MkG1PVI4QDh:L3G+
Shh-light2 MXTGeY5kfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKFOvbz3t[YRq[XSnZDDIbEB{cWewYXzpcochcW5iaIXtZY4hW2iqLXzp[4h1OiClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlMh|ryPLh?= M3fxPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[4OVUyLz5{MkK2PFU2OTxxYU6=
M210B4 MkDuSpVv[3Srb36gZZN{[Xl? NGTV[YwzPCCqcoO= NXvVZ2FZUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegbY4hdW:3c3WgUVIyOEJ2IHPlcIx{KGG|c3Xzd4VlKGG|IHTve45z\We3bHH0bY9vKG:oIGD0Z4ghdVKQQTDlfJBz\XO|aX;uJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNuIFnDOVAhRSByLkSzJO69VS5? MomwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO|E3RC:jPh?=
Shh-Light 2 M2PS[mZ2dmO2aX;uJIF{e2G7 NF\0NZAzKGSjeYO= NEDuRoRCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IGPtc{BqdiCvb4Xz[UBUcGhvTHnnbJQhOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHNpcC2rbnT1Z4VlKEeuaUGtdoVxd3K2ZYKgZYN1cX[rdImgZYZ1\XJiMjDkZZl{KGK7IHT1ZYwudHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJI1mfGixZDygTWM2OCB;IECuOFg1KM7:TT6= NYnwSIM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlM2OjJpPkKzNFY{PTJ{PD;hQi=>
Shh Light2 M2\Zd2Z2dmO2aX;uJIF{e2G7 NXXVbWpiUW6qaXLpeIlwdiCxZjDTTGghcW5ibX;1d4UhW2iqIFzp[4h1OiClZXzsd{BjgSCJTFmtdoV{eG:wc3n2[UBncXKnZnz5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwNTFOwG0v M3i4W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{C5NFgxLz5zOUOwPVA5ODxxYU6=
C3H10T1/2 Mo\vSpVv[3Srb36gZZN{[Xl? MmTzTY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIGPITEBi[3SrdnH0[YQheGG2aIfhfUBqdiCvb4Xz[UBEO0hzMGSxM|Ih[2WubIOgZZN{\XO|ZXSgZZMhW0GJLXnu[JVk\WRiY3XscEBlcW[oZYLlcpRq[XSrb36gZpkh[WytYXzpcoUheGixc4DoZZRie2ViYYPzZZktKEmFNUCgQUAxNjZizszNMi=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV7Mke4PEc,OjF3OUK3PFg9N2F-
C3H10T1/2 MXPGeY5kfGmxbjDhd5NigQ>? MYq2JIhzew>? MXXJcohq[mm2aX;uJI9nKFODRz3pcoR2[2WmIHTp[oZmemWwdHnheIlwdiCxZjDtc5V{\SCvZYPlcoNpgW2jbDDwcJVzcXCxdHXueEBEO0hzMGSxM|Ih[2WubIOgeI8h[WytYXzpcoUheGixc4DoZZRie2VicH;zbZRqfmVib3Xz[Y9jdGG|dIOgZYZ1\XJiNjDodpMtKEmFNUCgQUAxNjZ{IN88UU4> NFP1Sow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2PFU2OSd-MkKyOlg2PTF:L3G+
ASZ001 M4nzTGZ2dmO2aX;uJIF{e2G7 MmP4OFghcHK| NHjmWItKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDBV3oxODFiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiR3zpNUBuWk6DIHX4dJJme3Orb36gZYZ1\XJiNEigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyxiSVO1NEA:KDBwNk[g{txONg>? NX74OpkzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4OTZpPkK0PVAxPzF4PD;hQi=>
M210B4 NXm3c3NRTnWwY4Tpc44h[XO|YYm= NEjaZYczPCCqcoO= MlrxTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdiaX6gcY92e2ViTUKxNGI1KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjhizszNMi=> NFqwc5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFcyPid-MkS5NFA4OTZ:L3G+
medulloblastoma cells M2f4WGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKG2nZIXscI9jdGG|dH;tZUBk\WyuczDoZZJjd3KrbnegbIV1\XKxeonnc5V{KHC2Y3ixJIdmdmViYomgUXRVKGG|c3H5MEBGSzVyIE2gNUDPxE1w MnXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N|EoRjF5NEG3OlMyRC:jPh?=
CHO MYXGeY5kfGmxbjDhd5NigQ>? M2\PTGFvfGGpb37pd5Qh[WO2aY\peJkh[XRibX;1d4UhW22xIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[nliW{PIYWhpNUGpIHLpcoRqdmdiYYPzZZktKEmFNUCgQUAyNjJizszNMi=> NF\IZ5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC5NVU2QSd-MUmwPVE2PTl:L3G+
Shh-Light2 NFLMS5ZHfW6ldHnvckBie3OjeR?= NELke2c1QCCqcoO= M3[zZWlvcGmkaYTpc44hd2ZiU1jIJIlvKG2xdYPlJHNpcC2OaXfoeFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IFfsbVEhemWyb4L0[ZIh\2WwZTDhd5NigSCrbjDwdoV{\W6lZTDv[kBUSUduIFnDOVAhRSBzLkOxNkDPxE1w MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV2MUS5NEc,OTl3NEG0PVA9N2F-
MEF NUTvNJhITnWwY4Tpc44h[XO|YYm= MVrJcohq[mm2aX;uJI9nKFOvbzDpckBud3W|ZTDQeINpNWSnZnnjbYVvfCCPRV[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCVaHitbY5lfWOnZDDHcIkyKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUwhUUN3MDC9JFEvPSEQvF2u M4HVWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEe0OVQyLz5{M{C3OFU1OTxxYU6=
MEF MnPySpVv[3Srb36gZZN{[Xl? MnzSN|AhcHK| MoTsTY5pcWKrdHnvckBw\iCVbX:gbY4hdW:3c3WgVJRkcC2mZX\pZ4lmdnRiTVXGJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiU3joMYlv\HWlZXSgS4xqNXKnc4DvcpNqfmViYnX0ZYdidGGldH;zbYRie2ViYXP0bZZqfHliYX\0[ZIhOzBiaILzJIJ6KEKndHHHUI8h[XO|YYmsJGlEPTBiPTCxMlkh|ryPLh?= NVfidIo4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|Q2PDFpPkKzNFc1PTRzPD;hQi=>
neural precursor cells NF;Kd2VCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKG6ndYLhcEBxemWldYLzc5Ih[2WubIOgZpkh[2:ub375JIZwem2jdHnvckBie3OjeTygSWM2OCB;IEGzMlQ1KM7:TT6= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N|E9N2F-
U87 NHz2RpBEgXSxdH;4bYNqfHliYYPzZZk> NUH1XmRoS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTh5IHPlcIx{KGG|c3Xzd4VlKGG|II\pZYJqdGm2eTDpckBxemW|ZX7j[UBw\iCkZYThJIdtfWO3cn;ubYRie2VuIFnDOVAhRSBzNT61JO69VS5? NEXhcZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGxOlkxPCd-MkCxNVY6ODR:L3G+
U87MG M{LBVWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M2XHWVczKGi{cx?= NX7ZZY9vSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFdOTyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyIE2gNlIvPSEQvF2u NGfx[489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKyOlY2Pyd-MkKyNlY3PTd:L3G+
A549 M{fpNmFvfGmlYX7j[ZIh[XO|YYm= NXjPW4x3SW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[nliTWTTJIF{e2G7LDDJR|UxKD1iNEmg{txONg>? M13aUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkKxPFczLz5zOEKyNVg4OjxxYU6=
DU145 NIPxe5BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXzCRZFEPSC3TR?= MXi5OkBpenN? M3Hj[Wdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGRWOTR3IHPlcIx{KGG2IEWgeW0h[W[2ZYKgPVYhcHK| NF7wSYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0PVEzPSd-MUiyOFkyOjV:L3G+
DU145 NGjWWXBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mkf4NVAhfU1? MVi5OkBpenN? Mmf1S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[XRiMUCgeW0h[W[2ZYKgPVYhcHK| MnnHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEmxNlUoRjF6MkS5NVI2RC:jPh?=
HEK293 NVrseVlOTnWwY4Tpc44h[XO|YYm= MkPWTY5pcWKrdHnvckBw\iCkZYThJIdidGGldH;zbYRie2ViaX6gTGVMOjl|IHPlcIx{ MmnGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OUS3OlYoRjF5NEm0O|Y3RC:jPh?=
22Rv NXXlXpJSTnWwY4Tpc44h[XO|YYm= M{DoTXJm\HWldHnvckBw\iCneIDy[ZN{cW:wIH;mJHBVS0hibWLORUBqdiCqdX3hckAzOlK4IHPlcIx{ M{DIZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEm0O|Y3Lz5zN{S5OFc3PjxxYU6=
PANC1 NI\MUItHfW6ldHnvckBie3OjeR?= MYCwMlIhfU1? Mn\tNlQhcHK| NGrRboxKdmirYnn0bY9vKG:oIFjoM2dNUTFvbXXkbYF1\WRiUGTDTEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gVGFPSzFiY3XscJMh[XRiMD6yJJVOKGGodHXyJFI1KGi{czDifUBTXC2SQ2K= M2HSe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWwNVcxLz5{MES1NFE4ODxxYU6=
PANC1 M1m4XmZ2dmO2aX;uJIF{e2G7 M2rPflAvPCC3TR?= MXqyOEBpenN? M1TUdGlvcGmkaYTpc44hd2ZiSHivS2xKOS2vZXTpZZRm\CCSVFPIJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDQRW5EOSClZXzsd{BifCByLkSgeW0h[W[2ZYKgNlQhcHK|IHL5JHJVNVCFUh?= MlTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUCxO|AoRjJyNEWwNVcxRC:jPh?=
Shh Light2 NYm2dIdQTnWwY4Tpc44h[XO|YYm= NGraS3c3NjJ3IIXN NIPpUXA{OCCqcoO= MmW4TY5pcWKrdHnvckBw\iCQLYDhcI1qfG:7bHH0[YQhW2iqIHnuJI1wfXOnIGPobEBNcWeqdEKgZ4VtdHNiYYSgOk4zPSC3TTDh[pRmeiB|MDDodpMh[nliZnny[YZtgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF3MUezNUc,OTlzNUG3N|E9N2F-
U87MG Ml\PSpVv[3Srb36gZZN{[Xl? MXOxNEB2VQ>? Mn6yOEBpenN? MliwTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDoeY1idiCXOEfNS{Bk\WyuczDhd5Nme3OnZDDhd{Bld3ewIILl[5Vt[XSrb36gc4YhT2yrMTDheEAyOCC3TTDh[pRmeiB2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM> NU\kOpM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlY3PTdpPkKyNlI3PjV5PD;hQi=>
HEK293 NVLmXHNqTnWwY4Tpc44h[XO|YYm= MUW1JJVO MkTwNVAhcHK| NFK3XndFcXOybHHj[Y1mdnRib3[gRm9FUVC\LXP5Z4xweGGvaX7lJIZzd21iaIXtZY4hW22xIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYSgOUB2VSCvZXHzeZJm\CCjZoTldkAyOCCqcoOgZpkhTEGSSTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgcYlkem:|Y3;wbYMh[XO|YYm= NYmzTY5bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3N|YxPjNpPkK3O|M3ODZ|PD;hQi=>
HEK293 NVqw[m9HTnWwY4Tpc44h[XO|YYm= Mn\hOUB2VQ>? NEPPcZMyOCCqcoO= NUnzR25NTGm|cHzhZ4Vu\W62IH;mJGJQTEmSWT3jfYNtd3CjbXnu[UBnem:vIHj1cYFvKFOvbzDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHH0JFUhfU1ibXXhd5Vz\WRiYX\0[ZIhOTBiaILzJIJ6KE[DQ2OgZY5idHm|aYO= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd|NkC2N{c,Ojd5M{[wOlM9N2F-
C3H10T1/2 NHq1WlBHfW6ldHnvckBie3OjeR?= NUPIN5pnPTVwNTD0c{A1PTByIH7N Mof1R49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDTcY8hcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV2FINWmwZIXj[YQhT2yrMTD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgOVUvPSC2bzC0OVAxKG6PIHL5JJFRS1JiYX7hcJl{cXN? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
U2OS MXjGeY5kfGmxbjDhd5NigQ>? NUfwNnJEPSC3TR?= MljsOkBpenN? NH;wVINCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHPobY1memmlIGPtc{A3OzNibYX0ZY51KGW6cILld5Nm\CCrbjDVNm9UKGOnbHzzJINw\XiycnXzd4lv\yCkZYThJIFzemW|dHnuNk1ITlBiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTyZYNmdGy3bHHyJIJmfGFiYYLy[ZN1cW5{LVfGVEBi\2e{ZXfheIUh\m:{bXH0bY9vKGG2IEWgeW0h[W[2ZYKgOkBpenNiYomgZ49v\m:lYXygcYlkem:|Y3;wfS=> NIjk[5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2N|UzOid-MkOwOlM2OjJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB / Cyclin D1 / MMP2 / MMP9; 

PubMed: 23599805     


Whole cell lysates were prepared, and 50 μg proteins were resolved using SDS-PAGE, followed by immunoblotting with the indicated specific antibodies against NF-κB, cyclin D1, MMP2 and MMP9. 

Gli1 / TGF-β1 / CXCR4; 

PubMed: 26137001     


Western blotting demonstrated that Gli1, TGF-β1 and CXCR4 protein expression levels were downregulated in the AGS cells treated with cyclopamine for 24 h.

Snail / E-cadherin / Slug / Vimentin ; 

PubMed: 26859575     


SW1736 and WRO82 cells were incubated in the presence of vehicle (0.5% DMSO) or the indicated concentrations of cyclopamine or GANT61 for 72 hr. The cells were harvested and analyzed for the expression of several genes involved in EMT.

23599805 26137001 26859575
Immunofluorescence
Vimentin; 

PubMed: 24553082     


Vimentin protein expression was reduced following treatment with cyclopamine (20 μmol/L; 72 h), detected by immunocytochemistry.

PCNA / β-catenin; 

PubMed: 28747625     


Immunofluorescence images demonstrating the distribution of PCNA or β-catenin in EH cells pre-treated with cyclopamine in presence and absence of estrogen for 24 h. Experiments were repeated at least three times.

24553082 28747625
In vivo Administration of Cyclopamine at dose of 50 mg/kg/day for 22 days eradicates the HUCCT1 xenografts in mice with no obvious adverse effects. [2] Cyclopamine treatment at dose of 1.2 mg for 7 days induces significant apoptosis of tumor cells and decreases the tumor mass by 50-60% in Panc 05.04- and L3.6sl-derived tumors, respectively, but not in the BxPC3-SMOlow tumors. [3] Administration of Cyclopamine alone profoundly inhibits tumor metastases in xenografts of E3LZ10.7 and L3.6pl, and completely abrogates metastases when in combination of gemcitabine. [4]

Protocol

Kinase Assay:[1]
- Collapse

Hedgehog cell assay:

This assay measures the end stage of the Hh signaling pathway, that is, the transcriptional modulation of Gli, using Luciferase as readout (Gli-Luc assay). Cyclopamine is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are resuspended in F12 Ham's/DMEM (1:1) containing 5% FBS and 15 mM Hepes pH 7.3, added to assay plates and incubated with Cyclopamine for approximately 30 minutes at 37 °C in 5% CO2. 1 nM Hh-Ag 1.5 is then added to assay plates and incubated at 37 °C in the presence of 5% CO2. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 value, defined as the inflection point of the logistic curve, is determined by non-linear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of Cyclopamine using the R statistical software pack
Cell Research:[2]
- Collapse
  • Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, and HCT116
  • Concentrations: Dissolved in DMSO, final concentration 3 μM
  • Incubation Time: 4 days
  • Method: Cells are exposed to Cyclopamine in 96-well plates. Cell viability is measured by MTS (soluble tetrazolium salt) assay. Viable cell mass is determined by optical density measurements at 490 nm (OD490) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)﹣OD (day 2)/OD (day 2).
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: Athymic (nude) mice inoculated subcutaneously with HUCCT1 cells
  • Dosages: 50 mg/kg/day
  • Administration: Subcutaneous injection
    (Only for Reference)

Solubility (25°C)

In vitro DMF 10 mg/mL warmed (24.29 mM)
Ethanol 2 mg/mL warmed (4.85 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 411.62
Formula

C27H41NO2

CAS No. 4449-51-8
Storage powder
in solvent
Synonyms 11-deoxojervine
Smiles CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5CC4=C3C)C)O)C)NC1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How to reconstitute the compound for in vivo use in mice?

  • Answer:

    One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

Related Hedgehog/Smoothened Products

Tags: buy Cyclopamine | Cyclopamine supplier | purchase Cyclopamine | Cyclopamine cost | Cyclopamine manufacturer | order Cyclopamine | Cyclopamine distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID