Cyclopamine

For research use only.

Catalog No.S1146 Synonyms: 11-deoxojervine

94 publications

Cyclopamine Chemical Structure

CAS No. 4449-51-8

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

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Selleck's Cyclopamine has been cited by 94 publications

Purity & Quality Control

Choose Selective Hedgehog/Smoothened Inhibitors

Biological Activity

Description Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.
Targets
Smoothened [1]
(TM3Hh12 cells)
46 nM
In vitro

Cyclopamine inhibits the Hedgehog signaling pathway with an IC50 of 46 nM, and blocks the activity of human Smo receptor expressed in CHO-K1 cells in [3H]Hh-Ag binding assay with an IC50 of 280 nM. [1] Cyclopamine significantly inhibits Hedgehog pathway activity in a dose-dependent manner in gut-derived tumor cell lines expressing Patched (PTCH) mRNA, and induces growth inhibition of those tumor cell lines by 75-95% at the concentration of 3 μM, but ineffective towards the colon tumor cells without PTCH mRNA expression, suggesting the effects of Cyclopamine treatment are Hedgehog pathway related rather than generally cytotoxic. [2] By blocking Hedgehog signaling through direct interaction with Smo, Cyclopamine (10 μM) inhibits the proliferation of SMOhigh Cyclopamine-responsive cell lines L3.6sl and Panc 05.04 by 75-80%, and increases the apoptosis by 2.5- to 3.5-fold, without affecting the BxPC3-SMOlow cell line. [3] Cyclopamine treatment significantly decreases of Snail mRNA and increasea E-cadherin transcripts in the E3LZ10.7 cell line. Independent of inhibition of cell growth, Cyclopamine treatment significantly inhibits the invasive phenotype of Hedgehog-dependent L3.6pl cells, causing a >500-fold reduction in the number of transmigrating cells, but not that of the Hedgehog-independent cell line Panc-1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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SNB75 M3;VVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfXUZZKSzVyPUKyNU46PCEQvF2= M{HNTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES4 M4DvU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfwTWM2OD1{MkOuO|g{KM7:TR?= M{TmXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KARPAS-422 NWrUSo5pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\HToxKSzVyPUKyPE4{PTJizszN MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1648 M{\Temdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFX6SmpKSzVyPUKyPU41QDlizszN NYLLNppnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ES6 MoW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1faO2lEPTB;MkO5MlQ{KM7:TR?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KNS-81-FD NGDTcWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTJ2MT6xPVch|ryP M3:wXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
JAR MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3f1XGlEPTB;MkW2MlIzPSEQvF2= NEXtO4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NB1 Mn3KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1j6V2lEPTB;Mk[wMlUyPiEQvF2= MlLzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-336MG NUn0ZldxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moj2TWM2OD1{NkCuOlk5KM7:TR?= M{jQZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BC-3 NFjCOI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTJ4NT6xO|gh|ryP MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HCC2218 M3H5bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DJfWlEPTB;Mk[2MlQyPSEQvF2= M2rIVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
TE-9 NW\VXHg1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonuTWM2OD1{Nk[uOlI4KM7:TR?= MmrqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LB1047-RCC MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\tSoNXUUN3ME2yOlYvPzV|IN88US=> M{PEPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
CTB-1 M2DYeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTJ4OT65O|Mh|ryP NV[2dmNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NB7 M3zXTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrS[YRKSzVyPUK3NUDPxE1? NVzBdINDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ST486 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\hSGlEPTB;Mke3MlQyOiEQvF2= Moi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HCC1187 M3;0SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLLUnRKSzVyPUK4Nk45OTFizszN NIL1dmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NCI-SNU-16 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHVTWM2OD1{OESuNlQ5KM7:TR?= MmGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
COR-L279 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVf4N2hRUUN3ME2yPVEvPTh2IN88US=> NF3kSG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ES8 M3jTcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3jwS2lEPTB;Mkm0MlE5OiEQvF2= M3Gz[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
U-698-M M33IV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTJ7OD6yOFMh|ryP NX\VN2diRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HEL NVX5em5lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTNyOT6xOFkh|ryP NHrqd2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KINGS-1 NWS1ToZrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDQeG9KSzVyPUOxNE43PzRizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KY821 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nwR2lEPTB;M{O2MlU6PSEQvF2= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MZ1-PC M4DRUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLPTWM2OD1|NEWuOlE5KM7:TR?= M1:4eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LS-411N MoOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\xN2lEPTB;M{W0MlY3KM7:TR?= M{TseFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SIG-M5 NHPyOYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPaNlFKSzVyPUO1PU44QDJizszN NGLPflg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HT MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTN4Nz63NVEh|ryP M3LyVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HC-1 MmTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjpVHhKSzVyPUO2O{44QDdizszN M3Xs[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-H1694 NUjte3hOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljOTWM2OD1|N{KuPVM1KM7:TR?= M3TO[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BB65-RCC M1m3[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXzbXNrUUN3ME2zO|YvOjR3IN88US=> MojMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HAL-01 MlPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYWzTXg1UUN3ME2zO|kvQDN6IN88US=> MkD0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ARH-77 Mo\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDuOZl3UUN3ME2zPVQvODB6IN88US=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MZ7-mel NY\a[3FIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfOfXVKSzVyPUO5O{4zOzNizszN MnHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SIMA MnHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE[x[XlKSzVyPUSwN{46OzNizszN MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DG-75 NX;kVYF5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjkTWM2OD12MUWuOlk5KM7:TR?= NHvYe5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HUTU-80 MlWxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXaSnVKSzVyPUSxPU4yQDVizszN M3;aTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KNS-42 M{\iPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrUUXE6UUN3ME20NlUvQDF3IN88US=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
SH-4 M4jlZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXYTWM2OD12MkeuOVY2KM7:TR?= Mo[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
L-540 MlrJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTR|MT6wN|Eh|ryP M3XZOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NB10 M3G4bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;sUGlEPTB;NESxMlI{PCEQvF2= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES1 NHOyfnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nsXmlEPTB;NEWyMlc2OyEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KMOE-2 NV2xWHlCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjJT3FoUUN3ME20OVYvPzFzIN88US=> MoH3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MC116 NWPVfodmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfWTWM2OD12NUiuNVE3KM7:TR?= NHn4[Hc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
RCC10RGB Mm\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTR4MD6wNFUh|ryP MoX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RL95-2 NHHudXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nHcGlEPTB;NE[wMlI{PyEQvF2= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
Raji MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXQTWM2OD12NkiuNVQ{KM7:TR?= NUX2b4g2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
CAS-1 M{S4S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mki3TWM2OD12N{KuNFc{KM7:TR?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
Calu-6 NXrJcXVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\I[2lEPTB;NEe1MlI3PSEQvF2= NVXzdoxJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KG-1 Mn3FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWn0Z3JyUUN3ME20O|gvPDRizszN NU[wdIt6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LB771-HNC MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1O3[GlEPTB;NEiyMlI{OiEQvF2= MofsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ACN NEXOTJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LK[GlEPTB;NEmzMlU6QSEQvF2= NUTqV5Z6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KM12 NFnCZlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXixNHRnUUN3ME20PVYvPTh7IN88US=> M1PjcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
U2OS NV\GdVFSTnWwY4Tpc44h[XO|YYm= MnfxRolv\GmwZzDh[oZqdmm2eTD0c{B4cWymIIT5dIUhW22xIHX4dJJme3OnZDDpckBWOk:VIHPlcIx{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEumIE2gNE4xOTJ2IN88UU4> M{nrTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[zOVIzLz5{M{C2N|UzOjxxYU6=
U2OS MX;GeY5kfGmxbjDhd5NigQ>? MX:yJIhzew>? NFHQZ25FcXOybHHj[Y1mdnRib3[gX|NJZWO7Y3zvdIFucW6nIH\yc40hf2muZDD0fZBmKFOvbzDlfJBz\XO|ZXSgbY4hXTKRUzDj[YxteyCjZoTldkAzKGi{czDifUB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbUA:KDBwMEGyO{DPxE1w Ml3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ|ME[zOVIzRC:jPh?=
TM3 M4LLfWZ2dmO2aX;uJIF{e2G7 M{jlT|Q5KGi{cx?= NYjSNZJ5UW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDUUVMh[2WubIOgZoViemmwZzDwWGEuQHiJbHmtUJVkKHKncH;yeIVzKGOxboP0dpVkfCCjc4Pld5Nm\CCjczD0doFve2O{aYD0bY9v[WxibX;keYxifGmxbjDv[kBIdGliYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2ViYYPzZZktKEmFNUCgQUAxNjB2NjFOwG0v NX70NI1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPVE2PTlpPkG5NFkyPTV7PD;hQi=>
HCC827 NGD0O3NHfW6ldHnvckBie3OjeR?= NGPXcmpFcXOybHHj[Y1mdnRib3[gX|NJZS2leXPsc5BidWmwZTDmdo9uKFOPTzDWOFA1VSCvdYThcpQhcW5iZ3XmbZRqdmmkIILld4l{fGGwdDDoeY1idiCKQ1O4Nlch[2WubIOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSByLkC1NUDPxE1w NGDaPGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe4O|E2Pid-Mki3PFcyPTZ:L3G+
HEK293 M1XQNmZ2dmO2aX;uJIF{e2G7 M3fnb|IhcHK| M3\i[GRqe3CuYXPlcYVvfCCxZjDCU2RKWFlvbHHi[Yxt\WRiY4njcI9x[W2rbnWg[pJwdSCqdX3hckBUdW9icnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAzKGi{czDifUBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yeTygTWM2OCB;IECuNFY1KM7:TT6= NV7mUZJrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlg2PTFpPkKyNlY5PTVzPD;hQi=>
Shh Light2 NHPl[FBHfW6ldHnvckBie3OjeR?= M4PZ[|MxKGi{cx?= MYXJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIH3veZNmKFOqaDDMbYdpfDJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB{d26rYzDo[YRo\WixZz3pcoR2[2WmIFfMTVEudWWmaXH0[YQhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JI1m[XO3cnXkJIFnfGW{IEOwJIhzeyCkeTDkeYFtKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFc1OSEQvF2u NV\5[JBjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1Olc{PzFpPkK3OVY4OzdzPD;hQi=>
U2OS M1vvO2Z2dmO2aX;uJIF{e2G7 MlXwRolv\GmwZzDh[oZqdmm2eTD0c{BUdW9iRES3N2ghdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFvkJF0hOC5zMU[g{txONg>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
DaOY NFPrUnRHfW6ldHnvckBie3OjeR?= NXzGVoVNPDhiaILz MVHJcohq[mm2aX;uJI9nKEinZHflbI9oKHOrZ37hcIlv\yCrbjDoeY1idiCGYV;ZJINmdGy|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJGdtcTFibWLORUBmgHC{ZYPzbY9vKGGodHXyJFQ5KGi{czDifUBTXC2SQ2KgZY5idHm|aYOsJGlEPTBiPTCwMlE3KM7:TT6= NEfnV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFcyPid-MkS5NFA4OTZ:L3G+
U2OS M4\tUmZ2dmO2aX;uJIF{e2G7 NVj1[IsxOiCqcoO= MV3EbZNxdGGlZX3lcpQhd2ZiW{PIYYN6[2yxcHHtbY5mKG[{b32gV41wKER2N{PIJI12fGGwdDDlfJBz\XO|ZXSgbY4hXTKRUzDj[YxteyCjZoTldkAzKGi{czDifUB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbUA:KDBwMkOyJO69VS5? NXjTSIl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlM2OjJpPkKzNFY{PTJ{PD;hQi=>
CHO MlS2SpVv[3Srb36gZZN{[Xl? NFrYNoJCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKFOvbzDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{KGK7IGuzTH1JcC2DZzDibY5lcW6pIHHzd4F6NCCLQ{WwJF0hOC5{ODFOwG0v M2\NTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmxOVU6Lz5zOUC5NVU2QTxxYU6=
Shh Light2 MXTGeY5kfGmxbjDhd5NigQ>? NF3HdIQ1OCCqcoO= M1HROmlvcGmkaYTpc44hd2ZiU1jIJJBifGi5YYmgbY4hdW:3c3WgV4hpKEyrZ3j0NkBk\WyuczDh[pRmeiB2MDDodpMh[nliR3zpMYRmeGWwZHXueEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVAhRSByLkOg{txONg>? M2\LNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUmyO|g5Lz5{MUW5Nlc5QDxxYU6=
Shh-light2 MY\GeY5kfGmxbjDhd5NigQ>? NEXFTIlKdmirYnn0bY9vKG:oIGPtc{1u\WSrYYTl[EBJcCC|aXfuZYxqdmdiaX6gbJVu[W5iU3joMYxq\2i2MjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjNizszNMi=> NUHSbotyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlg2PTFpPkKyNlY5PTVzPD;hQi=>
M210B4 MUTGeY5kfGmxbjDhd5NigQ>? M17wTlI1KGi{cx?= NGj1XXFKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDNNlExSjRiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiUITjbEBuWk6DIHX4dJJme3Orb36gZYZ1\XJiMkSgbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyxiSVO1NEA:KDBwNEOg{txONg>? NHH2W3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFcyPid-MkS5NFA4OTZ:L3G+
Shh-Light 2 MXTGeY5kfGmxbjDhd5NigQ>? Ml3tNkBl[Xm| NEDzU|RCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IGPtc{BqdiCvb4Xz[UBUcGhvTHnnbJQhOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHNpcC2rbnT1Z4VlKEeuaUGtdoVxd3K2ZYKgZYN1cX[rdImgZYZ1\XJiMjDkZZl{KGK7IHT1ZYwudHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJI1mfGixZDygTWM2OCB;IECuOFg1KM7:TT6= NUKwUmtjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlM2OjJpPkKzNFY{PTJ{PD;hQi=>
Shh Light2 M1fTcmZ2dmO2aX;uJIF{e2G7 NH;MOmRKdmirYnn0bY9vKG:oIGPITEBqdiCvb4Xz[UBUcGhiTHnnbJQzKGOnbHzzJIJ6KEeOST3y[ZNxd26|aY\lJIZqemWobImgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC53IN88UU4> MlXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|MEmwPFAoRjF7M{C5NFgxRC:jPh?=
C3H10T1/2 MV3GeY5kfGmxbjDhd5NigQ>? M4CxTmlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDTTGgh[WO2aY\heIVlKHCjdHj3ZZkhcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W|c3XkJIF{KFODRz3pcoR2[2WmIHPlcIwh\GmoZnXy[Y51cWG2aX;uJIJ6KGGua3HsbY5mKHCqb4PwbIF1[XOnIHHzd4F6NCCLQ{WwJF0hOC54IN88UU4> M3HEPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUmyO|g5Lz5{MUW5Nlc5QDxxYU6=
C3H10T1/2 NXHQTIFJTnWwY4Tpc44h[XO|YYm= NIL5cXQ3KGi{cx?= M2r6SGlvcGmkaYTpc44hd2ZiU1HHMYlv\HWlZXSg[Iln\mW{ZX70bYF1cW:wIH;mJI1wfXOnIH3ld4Vv[2i7bXHsJJBtfXKrcH;0[Y51KEN|SEGwWFEwOiClZXzsd{B1dyCjbHvhcIlv\SCyaH;zdIhifGG|ZTDwc5NqfGm4ZTDv[ZNmd2KuYYP0d{Bi\nSncjC2JIhzeyxiSVO1NEA:KDBwNkKg{txONg>? MkT6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Nki1OVEoRjJ{Mk[4OVUyRC:jPh?=
ASZ001 MXXGeY5kfGmxbjDhd5NigQ>? MlTmOFghcHK| NFjJRZpKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDBV3oxODFiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiR3zpNUBuWk6DIHX4dJJme3Orb36gZYZ1\XJiNEigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyxiSVO1NEA:KDBwNk[g{txONg>? NHTVeGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFcyPid-MkS5NFA4OTZ:L3G+
M210B4 NIr1fldHfW6ldHnvckBie3OjeR?= M4DqVlI1KGi{cx?= M4HYdWlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJI1wfXOnIF2yNVBDPCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCJbHmxJI1TVkFiZYjwdoV{e2mxbjDh[pRmeiB{NDDodpMh[nliUmStVGNTKGGwYXz5d4l{NCCLQ{WwJF0hOC56IN88UU4> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEexOkc,OjR7MEC3NVY9N2F-
medulloblastoma cells NV\J[HFbSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M{nDT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUAyKM7:TT6= NFTZeIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxO|Y{OSd-MUe0NVc3OzF:L3G+
CHO Mo[1SpVv[3Srb36gZZN{[Xl? MX3BcpRi\2:waYP0JIFkfGm4aYT5JIF1KG2xdYPlJHNudyC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KFt|SG3IbE1C\yCkaX7kbY5oKGG|c3H5MEBKSzVyIE2gNU4zKM7:TT6= NH3NNXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC5NVU2QSd-MUmwPVE2PTl:L3G+
Shh-Light2 MVjGeY5kfGmxbjDhd5NigQ>? M3[5RlQ5KGi{cx?= MlnvTY5pcWKrdHnvckBw\iCVSFigbY4hdW:3c3WgV4hpNUyrZ3j0NkBk\WyuczDh[pRmeiB2ODDodpMh[nliR3zpNUBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJHNCTyxiSVO1NEA:KDFwM{GyJO69VS5? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV2MUS5NEc,OTl3NEG0PVA9N2F-
MEF NIPvXJNHfW6ldHnvckBie3OjeR?= MVzJcohq[mm2aX;uJI9nKFOvbzDpckBud3W|ZTDQeINpNWSnZnnjbYVvfCCPRV[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCVaHitbY5lfWOnZDDHcIkyKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUwhUUN3MDC9JFEvPSEQvF2u MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
MEF MXTGeY5kfGmxbjDhd5NigQ>? NHHqfIU{OCCqcoO= M2P4bmlvcGmkaYTpc44hd2ZiU33vJIlvKG2xdYPlJHB1[2hvZHXmbYNq\W62IF3FSkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGPobE1qdmS3Y3XkJGdtcS2{ZYPwc45{cX[nIHLleIFo[WyjY4Tvd4ll[XOnIHHjeIl3cXS7IHHmeIVzKDNyIHjyd{BjgSCEZYThS2xwKGG|c3H5MEBKSzVyIE2gNU46KM7:TT6= NFLG[Jg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3OFU1OSd-MkOwO|Q2PDF:L3G+
neural precursor cells Mo\KRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGHqfHdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIH7leZJidCCycnXjeZJ{d3JiY3XscJMh[nliY3;sc456KG[xcn3heIlwdiCjc4PhfUwhTUN3MDC9JFE{NjR2IN88UU4> MoXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N|EoRjF5NEG3OlMyRC:jPh?=
U87 M2K3[GN6fG:2b4jpZ4l1gSCjc4PhfS=> NV7lcFdkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTh5IHPlcIx{KGG|c3Xzd4VlKGG|II\pZYJqdGm2eTDpckBxemW|ZX7j[UBw\iCkZYThJIdtfWO3cn;ubYRie2VuIFnDOVAhRSBzNT61JO69VS5? M1\LVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUG2PVA1Lz5{MEGxOlkxPDxxYU6=
U87MG NGD0[nlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MUG3NkBpenN? MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6N13HJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFnDOVAhRSB{Mj61JO69VS5? M2D3WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkK2OlU4Lz5{MkKyOlY2PzxxYU6=
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HEK293 MXLGeY5kfGmxbjDhd5NigQ>? NHLn[JRKdmirYnn0bY9vKG:oIHLleIEh\2GuYXP0c5Nq\GG|ZTDpckBJTUt{OUOgZ4VtdHN? M17tPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEm0O|Y3Lz5zN{S5OFc3PjxxYU6=
22Rv MVLGeY5kfGmxbjDhd5NigQ>? M3zSVHJm\HWldHnvckBw\iCneIDy[ZN{cW:wIH;mJHBVS0hibWLORUBqdiCqdX3hckAzOlK4IHPlcIx{ MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR7NEe2Okc,OTd2OUS3OlY9N2F-
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PANC1 M3K3OGZ2dmO2aX;uJIF{e2G7 NHu3[HkxNjRidV2= NGXHSJUzPCCqcoO= NFPlbXlKdmirYnn0bY9vKG:oIFjoM2dNUTFvbXXkbYF1\WRiUGTDTEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gVGFPSzFiY3XscJMh[XRiMD60JJVOKGGodHXyJFI1KGi{czDifUBTXC2SQ2K= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MEG3NEc,OjB2NUCxO|A9N2F-
Shh Light2 NWDNc49yTnWwY4Tpc44h[XO|YYm= NGHqfpc3NjJ3IIXN NUCzXFhYOzBiaILz MVjJcohq[mm2aX;uJI9nKE5vcHHscYl1d3muYYTl[EBUcGhiaX6gcY92e2ViU3joJGxq\2i2MjDj[YxteyCjdDC2MlI2KHWPIHHmeIVzKDNyIHjyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?= MnT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzNUG3N|EoRjF7MUWxO|MyRC:jPh?=
U87MG M1foUmZ2dmO2aX;uJIF{e2G7 MoTINVAhfU1? NI\QVZE1KGi{cx?= NUG0UYxrUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBpfW2jbjDVPFdOTyClZXzsd{Bie3Onc4Pl[EBieyCmb4fuJJJm\3WuYYTpc44hd2ZiR3zpNUBifCBzMDD1UUBi\nSncjC0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? NELmcGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKyOlY2Pyd-MkKyNlY3PTd:L3G+
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HEK293 MYXGeY5kfGmxbjDhd5NigQ>? NXTBZWF6PSC3TR?= NIr4ZmwyOCCqcoO= MXTEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XRiNTD1UUBu\WG|dYLl[EBi\nSncjCxNEBpenNiYomgSmFEWyCjbnHsfZNqew>? NHXzXIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ezOlA3Oyd-Mke3N|YxPjN:L3G+
C3H10T1/2 NGjzZ21HfW6ldHnvckBie3OjeR?= NHP0Row2PS53IITvJFQ2ODBibl2= NF\2fGhEd22yZYTpeIl3\SCrbnjpZol1cW:wIH;mJHNudyCrbjDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBUSUdvaX7keYNm\CCJbHmxJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDheEA2PS53IITvJFQ2ODBibl2gZpkheVCFUjDhcoFtgXOrcx?= NWDnXGNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|Q2PDFpPkKzNFc1PTRzPD;hQi=>
U2OS MnThSpVv[3Srb36gZZN{[Xl? MofJOUB2VQ>? MkC5OkBpenN? MWDBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGOqaX3ldolkKFOvbzC2N|MhdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy|IHPv[ZhxemW|c3nu[{Bj\XSjIHHydoV{fGmwMj3HSnAh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBqdnS{YXPlcIx2dGG{IHLleIEh[XK{ZYP0bY4zNUeIUDDh[4dz\WejdHWg[o9zdWG2aX;uJIF1KDVidV2gZYZ1\XJiNjDodpMh[nliY3;u[o9k[WxibXnjdo9{[2:yeR?= M2nNTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[zOVIzLz5{M{C2N|UzOjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Snail / E-cadherin / Slug / Vimentin ; 

PubMed: 26859575     


SW1736 and WRO82 cells were incubated in the presence of vehicle (0.5% DMSO) or the indicated concentrations of cyclopamine or GANT61 for 72 hr. The cells were harvested and analyzed for the expression of several genes involved in EMT.

Gli1 / TGF-β1 / CXCR4; 

PubMed: 26137001     


Western blotting demonstrated that Gli1, TGF-β1 and CXCR4 protein expression levels were downregulated in the AGS cells treated with cyclopamine for 24 h.

NF-κB / Cyclin D1 / MMP2 / MMP9; 

PubMed: 23599805     


Whole cell lysates were prepared, and 50 μg proteins were resolved using SDS-PAGE, followed by immunoblotting with the indicated specific antibodies against NF-κB, cyclin D1, MMP2 and MMP9. 

26859575 26137001 23599805
Immunofluorescence
PCNA / β-catenin; 

PubMed: 28747625     


Immunofluorescence images demonstrating the distribution of PCNA or β-catenin in EH cells pre-treated with cyclopamine in presence and absence of estrogen for 24 h. Experiments were repeated at least three times.

Vimentin; 

PubMed: 24553082     


Vimentin protein expression was reduced following treatment with cyclopamine (20 μmol/L; 72 h), detected by immunocytochemistry.

28747625 24553082
In vivo Administration of Cyclopamine at dose of 50 mg/kg/day for 22 days eradicates the HUCCT1 xenografts in mice with no obvious adverse effects. [2] Cyclopamine treatment at dose of 1.2 mg for 7 days induces significant apoptosis of tumor cells and decreases the tumor mass by 50-60% in Panc 05.04- and L3.6sl-derived tumors, respectively, but not in the BxPC3-SMOlow tumors. [3] Administration of Cyclopamine alone profoundly inhibits tumor metastases in xenografts of E3LZ10.7 and L3.6pl, and completely abrogates metastases when in combination of gemcitabine. [4]

Protocol

Kinase Assay:[1]
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Hedgehog cell assay:

This assay measures the end stage of the Hh signaling pathway, that is, the transcriptional modulation of Gli, using Luciferase as readout (Gli-Luc assay). Cyclopamine is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are resuspended in F12 Ham's/DMEM (1:1) containing 5% FBS and 15 mM Hepes pH 7.3, added to assay plates and incubated with Cyclopamine for approximately 30 minutes at 37 °C in 5% CO2. 1 nM Hh-Ag 1.5 is then added to assay plates and incubated at 37 °C in the presence of 5% CO2. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 value, defined as the inflection point of the logistic curve, is determined by non-linear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of Cyclopamine using the R statistical software pack
Cell Research:[2]
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  • Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, and HCT116
  • Concentrations: Dissolved in DMSO, final concentration 3 μM
  • Incubation Time: 4 days
  • Method: Cells are exposed to Cyclopamine in 96-well plates. Cell viability is measured by MTS (soluble tetrazolium salt) assay. Viable cell mass is determined by optical density measurements at 490 nm (OD490) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)﹣OD (day 2)/OD (day 2).
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Athymic (nude) mice inoculated subcutaneously with HUCCT1 cells
  • Dosages: 50 mg/kg/day
  • Administration: Subcutaneous injection
    (Only for Reference)

Solubility (25°C)

In vitro DMF 10 mg/mL warmed (24.29 mM)
Ethanol 2 mg/mL warmed (4.85 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 411.62
Formula

C27H41NO2

CAS No. 4449-51-8
Storage powder
in solvent
Synonyms 11-deoxojervine
Smiles CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5CC4=C3C)C)O)C)NC1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to reconstitute the compound for in vivo use in mice?

  • Answer:

    One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID