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Cyclopamine

Name: Cyclopamine
Brand: Selleck Chemicals
SKU: S1146
Rating: 5 (105 reviews)

Catalog No.S1146 Synonyms: 11-deoxojervine

For research use only.

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

CAS No. 4449-51-8

Selleck's Cyclopamine has been cited by 105 publications

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Hedgehog/Smoothened Signaling Pathway Map

Biological Activity

Description

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

Targets

Smoothened ^[1]
(TM3Hh12 cells)

46 nM

In vitro

Cyclopamine inhibits the Hedgehog signaling pathway with an IC50 of 46 nM, and blocks the activity of human Smo receptor expressed in CHO-K1 cells in [³H]Hh-Ag binding assay with an IC50 of 280 nM. ^[1] Cyclopamine significantly inhibits Hedgehog pathway activity in a dose-dependent manner in gut-derived tumor cell lines expressing Patched (PTCH) mRNA, and induces growth inhibition of those tumor cell lines by 75-95% at the concentration of 3 μM, but ineffective towards the colon tumor cells without PTCH mRNA expression, suggesting the effects of Cyclopamine treatment are Hedgehog pathway related rather than generally cytotoxic. ^[2] By blocking Hedgehog signaling through direct interaction with Smo, Cyclopamine (10 μM) inhibits the proliferation of SMO^high Cyclopamine-responsive cell lines L3.6sl and Panc 05.04 by 75-80%, and increases the apoptosis by 2.5- to 3.5-fold, without affecting the BxPC3-SMO^low cell line. ^[3] Cyclopamine treatment significantly decreases of Snail mRNA and increasea E-cadherin transcripts in the E3LZ10.7 cell line. Independent of inhibition of cell growth, Cyclopamine treatment significantly inhibits the invasive phenotype of Hedgehog-dependent L3.6pl cells, causing a >500-fold reduction in the number of transmigrating cells, but not that of the Hedgehog-independent cell line Panc-1. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

OS-RC-2	NYD3elJMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1fIfGlEPTB;NT64OlY3KM7:TR?=	NIW3Wo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
DOHH-2	MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MYLJR\|UxRTlwM{W2PFkh\|ryP	NVLLWJNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
no-10	Mn3uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M4nhNWlEPTB;OT65NFM6KM7:TR?=	M4\ZUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LS-513	MnHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MkSxTWM2OD1zMT6zOVQ4KM7:TR?=	M3nZXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ALL-PO	Mlm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MX\JR\|UxRTFzLke3N\|Qh\|ryP	MmLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
8-MG-BA	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NF3xSlFKSzVyPUGzMlEyOjNizszN	NVTybY4{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
RPMI-8402	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3PSbWlEPTB;MUWuPFU{PyEQvF2=	NFPkeGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
EoL-1-cell	MnvYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MnWzTWM2OD1zOD61PVQ5KM7:TR?=	NVrPSZY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NALM-6	NHexRoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M3;icmlEPTB;MUmuNFE3PyEQvF2=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DEL	MoG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NEP0dZpKSzVyPUKwMlE1PzFizszN	NWrQcmxzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SR	NYn1XYlET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NYXtblV2UUN3ME2yN{43PzF3IN88US=>	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
697	NXXqZ4pIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NIS2[YVKSzVyPUK2MlYyPTVizszN	M3zW[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
COLO-829	MonQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			Mm[zTWM2OD1{Nj64OFg{KM7:TR?=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
EVSA-T	M2rjeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M2LZSWlEPTB;MkeuOVU3OSEQvF2=	MmXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ATN-1	MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NX3IfGhUUUN3ME2zNU4zOzJ7IN88US=>	Ml36QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
L-363	NUW5[3I6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NWfFPJVoUUN3ME2zNU44PDZzIN88US=>	M1PQblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LAMA-84	M3u5[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NY\JfFhsUUN3ME2zNk42OjFzIN88US=>	MkfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NOS-1	NXLCboZVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1HObGlEPTB;M{SuNlk2PiEQvF2=	M2S1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BB30-HNC	NXTNRmFtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M13oSmlEPTB;M{SuN\|MxPiEQvF2=	Mm\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
BC-1	NHrHVohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			Mnm2TWM2OD1\|Nz65O\|Q3KM7:TR?=	MlS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
IST-SL2	NYjQTXo5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MWDJR\|UxRTN6LkKyOEDPxE1?	MmXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-392MG	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MUfJR\|UxRTRyLkKyNVUh\|ryP	NYqweJBrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
no-11	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MYrJR\|UxRTRyLkW1NlEh\|ryP	M1vKR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LC4-1	NY\jfXZzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1XzeGlEPTB;NECuPFcyPiEQvF2=	NXTobnVHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
A388	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NIriZ5dKSzVyPUSyMlU5PDhizszN	M{DqSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NTERA-S-cl-D1	NHrPe2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVfJR\|UxRTR{LkewO\|Qh\|ryP	M3:4R\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
CESS	NXPsXZJQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MX;JR\|UxRTR2LkKyN\|Ih\|ryP	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RS4-11	MlfJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MnOxTWM2OD12OT6wPVM5KM7:TR?=	MkP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MS-1	M2\ZN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MULJR\|UxRTVyLkmzOVEh\|ryP	NF\x[3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
CTV-1	NIfuNYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NU\zV\|I5UUN3ME21NU4xPzRizszN	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
D-502MG	NV3aZnFRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MnPETWM2OD13MT62NlcyKM7:TR?=	M1;vPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ML-2	NIPvb2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MXfJR\|UxRTV{LkmxPVUh\|ryP	M372elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SK-NEP-1	M4Dhc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MlTVTWM2OD13Mz6zPVI{KM7:TR?=	NFzRVXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LOXIMVI	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NXm0VGw2UUN3ME21N{42QDh2IN88US=>	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DJM-1	M4jEPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NV60VVlEUUN3ME21Ok4{OzlzIN88US=>	NH3xcIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
GI-1	Ml[2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NYW1cGtJUUN3ME21Ok43OTR7IN88US=>	NEmwTow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
IST-MES1	M2D4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MYnJR\|UxRTZyLkW0PVMh\|ryP	M1Xr[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MV-4-11	NEjVdolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MUXJR\|UxRTZyLk[1N\|gh\|ryP	Mm\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
OVCAR-4	M1Pyfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NIL6N\|JKSzVyPU[zMlU3PTdizszN	NFjWcnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KE-37	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3PUTGlEPTB;Nk[uNlY3QCEQvF2=	NGTuNHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
D-542MG	MnTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MVzJR\|UxRTZ6LkSxN\|Uh\|ryP	M3LzcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MHH-PREB-1	NX\scndVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M12wPWlEPTB;N{KuPFQ1OSEQvF2=	M33PPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MRK-nu-1	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NV7qSm9RUUN3ME23N{41PzB3IN88US=>	NUTQPYRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
D-247MG	NH[x[4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVnJR\|UxRTd\|LkW0OFIh\|ryP	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
OCI-AML2	NIHYT4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M2jXOmlEPTB;N{[uPVM3QSEQvF2=	NHrz[W89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
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KARPAS-45	MnKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXrJR\|UxRTh2Lk[5PVIh\|ryP	NVL6XlFVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
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MOLT-16	NIrvZVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnvOTWM2OD1zMESuPVg3KM7:TR?=	NYHLfGM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1770	M2LkZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGjqVGVKSzVyPUGwPE44QDRizszN	NYHySZdvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H82	MojWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MnTRTWM2OD1zMUCuPVc3KM7:TR?=	M2DhZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCCIT	NWOyb5hbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml7QTWM2OD1zMUKuOVI6KM7:TR?=	NUL6Z2hRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KALS-1	NYm1c5lPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MWnJR\|UxRTFzNT65OFEh\|ryP	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB2241-RCC	M4rCTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MXPJR\|UxRTFzNj62O\|kh\|ryP	NUS4PXdnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HH	NH7iXYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M4rzS2lEPTB;MUG3MlM6PSEQvF2=	NWC4fFRWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HD-MY-Z	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MmLlTWM2OD1zMUiuOFg5KM7:TR?=	NXvk[HVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
EB-3	MnTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M4TnOWlEPTB;MUKzMlA6PCEQvF2=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BL-70	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MV\JR\|UxRTF{Mz6xNlch\|ryP	M3f5c\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
K-562	NGP1S2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MWTJR\|UxRTF{Nj6yOFUh\|ryP	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HT-144	NVfvTXJsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1;yWGlEPTB;MUOzMlE3PCEQvF2=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
PF-382	MkjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MoDXTWM2OD1zM{SuN\|YyKM7:TR?=	Mm\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RPMI-8226	M{LVPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NHjYeotKSzVyPUGzOU4xPDVizszN	M{HvbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-H1355	Mmr3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MWTJR\|UxRTF\|NT61PFch\|ryP	NYLvbnB4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LXF-289	M37ke2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MYnJR\|UxRTF\|OT63PFEh\|ryP	M3\5R\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-H69	NVfF[5AyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MX7JR\|UxRTF2Mj65N\|Ih\|ryP	MmfaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SK-MEL-1	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{D4d2lEPTB;MUS3MlE{KM7:TR?=	M3\hW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KARPAS-299	NIDWSJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NGHScYJKSzVyPUG0PU4yOiEQvF2=	NEHzVnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
GB-1	NYHBUYhJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MVHJR\|UxRTF2OT6zNlIh\|ryP	Mlj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CMK	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NGiwdYdKSzVyPUG0PU42OTVizszN	NESwS4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
MPP-89	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M4rxSWlEPTB;MUW2MlA{PSEQvF2=	NHfzcXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KU812	M{PWVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MUfJR\|UxRTF4MT65NFIh\|ryP	Ml\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
REH	NFnvRZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MWnJR\|UxRTF4Mj6xNlUh\|ryP	NHTBNHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
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KP-N-YS	MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MmHvTWM2OD1zNkiuN\|k2KM7:TR?=	M1TiUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
Ramos-2G6-4C10	Ml\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NIW1SopKSzVyPUG2PU46OTVizszN	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
Becker	M4ju[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M2\FUGlEPTB;MUe0MlE5KM7:TR?=	NH:5S\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LB647-SCLC	NUXBeGl{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NED0S3ZKSzVyPUG3OU45PDVizszN	NFHnfYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LU-139	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NWrEc\|NCUUN3ME2xO\|gvODF7IN88US=>	NXfSRnM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
QIMR-WIL	Ml3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXvJR\|UxRTF5OT62OFYh\|ryP	NWXRS4p4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1395	NGnDT3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MXnJR\|UxRTF5OT65PVYh\|ryP	M1LCWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NOMO-1	M3i0Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NYXabVA1UUN3ME2xPFIvQDVizszN	M3vuOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
GI-ME-N	M3z3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MVTJR\|UxRTF6Nz65Olkh\|ryP	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KMS-12-PE	MmW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MYLJR\|UxRTF6OT6yO\|Mh\|ryP	NXfZZ49KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
Daudi	M3u5cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NWfjRYI6UUN3ME2xPVEvOTJ6IN88US=>	NI[yOYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LB996-RCC	M3qx[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M1r5ZmlEPTB;MUmxMlY6QSEQvF2=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H2107	NGTMXopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NVnyVWJMUUN3ME2xPVMvPzN7IN88US=>	NVT0eYNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SK-PN-DW	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NFvmVGhKSzVyPUG5OE44OTlizszN	MnTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MC-CAR	NUPofYhYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXnJR\|UxRTJyMj6yOVMh\|ryP	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
SNB75	NHrsN\|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVTJR\|UxRTJ{MT65OEDPxE1?	NWXvSoJZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ES4	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MXPJR\|UxRTJ{Mz63PFMh\|ryP	NVvuWWJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KARPAS-422	NIDPe3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnPXTWM2OD1{MkiuN\|UzKM7:TR?=	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1648	NWjIcI5kT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MljwTWM2OD1{MkmuOFg6KM7:TR?=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES6	MnvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MmH5TWM2OD1{M{muOFMh\|ryP	NWT3NohRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KNS-81-FD	NFvDeYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M{T0TmlEPTB;MkSxMlE6PyEQvF2=	MnO4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
JAR	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MnrFTWM2OD1{NU[uNlI2KM7:TR?=	NY[1[I41RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NB1	M3HNOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MVPJR\|UxRTJ4MD61NVYh\|ryP	MlrQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-336MG	Ml3FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NW\sdZV4UUN3ME2yOlAvPjl6IN88US=>	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BC-3	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M1LzUGlEPTB;Mk[1MlE4QCEQvF2=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HCC2218	NH30SmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MV\JR\|UxRTJ4Nj60NVUh\|ryP	MkfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
TE-9	NXTPOJdrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NXXUTJdUUUN3ME2yOlYvPjJ5IN88US=>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB1047-RCC	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NWr3bIlHUUN3ME2yOlYvPzV\|IN88US=>	NYDNfWNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
CTB-1	NH72TpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M3XhRWlEPTB;Mk[5Mlk4OyEQvF2=	Mn\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NB7	NEfxcXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NGfzWWlKSzVyPUK3NUDPxE1?	NFu1SHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
ST486	NIX1OFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MmLFTWM2OD1{N{euOFEzKM7:TR?=	M370XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HCC1187	NHrPT2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MlW3TWM2OD1{OEKuPFEyKM7:TR?=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-SNU-16	NHnTWIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NXSwV4l4UUN3ME2yPFQvOjR6IN88US=>	NYDnXVB6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
COR-L279	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			Ml30TWM2OD1{OUGuOVg1KM7:TR?=	M3uwUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES8	NUjLeIJjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml7TTWM2OD1{OUSuNVgzKM7:TR?=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
U-698-M	NIjNUpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NUDQcZFQUUN3ME2yPVgvOjR\|IN88US=>	NHjROFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
HEL	NE[y[plIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MYXJR\|UxRTNyOT6xOFkh\|ryP	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KINGS-1	NWHLRmJGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NWfqdmNEUUN3ME2zNVAvPjd2IN88US=>	NGLubm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KY821	MlG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NY\BbYh[UUN3ME2zN\|YvPTl3IN88US=>	MlPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ1-PC	NGKxOVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M1zzOWlEPTB;M{S1MlYyQCEQvF2=	MknDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LS-411N	MlfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NYKxbmJkUUN3ME2zOVQvPjZizszN	NXvYW5F6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SIG-M5	M2\hRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NUHkOIp1UUN3ME2zOVkvPzh{IN88US=>	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HT	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NHG5SW9KSzVyPUO2O{44OTFizszN	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HC-1	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NITrc\|FKSzVyPUO2O{44QDdizszN	MnTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NCI-H1694	M3zFfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3;oSGlEPTB;M{eyMlk{PCEQvF2=	NGPjc2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
BB65-RCC	MojhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NVjoSHNWUUN3ME2zO\|YvOjR3IN88US=>	NFzXS4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
HAL-01	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MonxTWM2OD1\|N{muPFM5KM7:TR?=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ARH-77	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{DkXWlEPTB;M{m0MlAxQCEQvF2=	MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ7-mel	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{PiRWlEPTB;M{m3MlI{OyEQvF2=	NYnZSHZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SIMA	MnzoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			Mn;zTWM2OD12MEOuPVM{KM7:TR?=	NIPFVYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
DG-75	Mn3qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NH\zVY5KSzVyPUSxOU43QThizszN	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HUTU-80	Mkf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			Mk\PTWM2OD12MUmuNVg2KM7:TR?=	NH3HTWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KNS-42	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MV3JR\|UxRTR{NT64NVUh\|ryP	M1H3S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SH-4	M3\ORWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NEXLbJhKSzVyPUSyO{42PjVizszN	NH7rWXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
L-540	NFjOR2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVzJR\|UxRTR\|MT6wN\|Eh\|ryP	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB10	NF;aSWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NXKxUpg{UUN3ME20OFEvOjN2IN88US=>	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES1	MkThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MVfJR\|UxRTR3Mj63OVMh\|ryP	M3X1[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KMOE-2	NHjTelVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MUDJR\|UxRTR3Nj63NVEh\|ryP	M{K2WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MC116	MnHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NEPsZZlKSzVyPUS1PE4yOTZizszN	M4K2RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
RCC10RGB	NGnzd21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MkTOTWM2OD12NkCuNFA2KM7:TR?=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RL95-2	Mm[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M3rtemlEPTB;NE[wMlI{PyEQvF2=	MnrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
Raji	NW\GUYNYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M324PGlEPTB;NE[4MlE1OyEQvF2=	Ml7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CAS-1	NVPWVHY{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M3:1UmlEPTB;NEeyMlA4OyEQvF2=	MnXvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
Calu-6	NHTifFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MXHJR\|UxRTR5NT6yOlUh\|ryP	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KG-1	MknKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M3:yO2lEPTB;NEe4MlQ1KM7:TR?=	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB771-HNC	NIrDO2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnHVTWM2OD12OEKuNlMzKM7:TR?=	M4PUTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ACN	NULZUWo2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M4PYPWlEPTB;NEmzMlU6QSEQvF2=	NE\IVGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KM12	M1q1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NVnZWop2UUN3ME20PVYvPTh7IN88US=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
U2OS	MmSzSpVv[3Srb36gZZN{[Xl?			NH7QWmNDcW6maX7nJIFn\mmwaYT5JJRwKHerbHSgeJlx\SCVbX:g[ZhxemW\|c3XkJIlvKFV{T2OgZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3SgQUAxNjBzMkSg{txONg>?	MkDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ\|ME[zOVIzRC:jPh?=
U2OS	Mlu2SpVv[3Srb36gZZN{[Xl?		MUWyJIhzew>?	NVG3WZV6TGm\|cHzhZ4Vu\W62IH;mJHs{UF2leXPsc5BidWmwZTDmdo9uKHerbHSgeJlx\SCVbX:g[ZhxemW\|c3XkJIlvKFV{T2OgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSByLkCxNlch\|ryPLh?=	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
TM3	NV7IcWh4TnWwY4Tpc44h[XO\|YYm=		MoH4OFghcHK\|	NEPa[mlKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIGTNN{Bk\WyuczDi[YFzcW6pIIDURU05gEeuaT3MeYMhemWyb4L0[ZIh[2:wc4TyeYN1KGG\|c3Xzd4VlKGG\|IITyZY5{[3KrcITpc45idCCvb3T1cIF1cW:wIH;mJGdtcSCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SCjc4PhfUwhUUN3MDC9JFAvODR4IN88UU4>	NVe2W3o5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPVE2PTlpPkG5NFkyPTV7PD;hQi=>
HCC827	NILI[ohHfW6ldHnvckBie3OjeR?=			MWPEbZNxdGGlZX3lcpQhd2ZiW{PIYU1kgWOub4DhcYlv\SCocn;tJHNOVyCYNEC0UUBufXSjboSgbY4h\2WoaYTpcoljKHKnc3nzeIFvfCCqdX3hckBJS0N6MkegZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3mgQUAxNjB3MTFOwG0v	Mo[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OEexOVYoRjJ6N{i3NVU3RC:jPh?=
HEK293	NXnYOmg1TnWwY4Tpc44h[XO\|YYm=		NXnyPYZOOiCqcoO=	NGrXRmhFcXOybHHj[Y1mdnRib3[gRm9FUVC\LXzhZoVtdGWmIHP5Z4xweGGvaX7lJIZzd21iaIXtZY4hW22xIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcImsJGlEPTBiPTCwMlA3PCEQvF2u	NIXzOXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2PFU2OSd-MkKyOlg2PTF:L3G+
Shh Light2	MojnSpVv[3Srb36gZZN{[Xl?		M1ezXFMxKGi{cx?=	MnHWTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC\|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCVaHigUIlocHR{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yhe2:waXOgbIVl\2Wqb3etbY5lfWOnZDDHUGkyNW2nZHnheIVlKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBu\WG\|dYLl[EBi\nSncjCzNEBpenNiYomg[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTBiPTCwMlA4PDFizszNMi=>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzV4N{O3NUc,Ojd3NkezO\|E9N2F-
U2OS	MoLrSpVv[3Srb36gZZN{[Xl?			MonURolv\GmwZzDh[oZqdmm2eTD0c{BUdW9iRES3N2ghdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFvkJF0hOC5zMU[g{txONg>?	MljHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ\|ME[zOVIzRC:jPh?=
DaOY	NHroXJlHfW6ldHnvckBie3OjeR?=		NXq3TW9pPDhiaILz	Ml;CTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC\|aXfuZYxqdmdiaX6gbJVu[W5iRHHPXUBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kBIdGlzIH3SUmEh\XiycnXzd4lwdiCjZoTldkA1QCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nzMEBKSzVyIE2gNE4yPiEQvF2u	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEexOkc,OjR7MEC3NVY9N2F-
U2OS	NHT5[ZNHfW6ldHnvckBie3OjeR?=		NWnZeG1GOiCqcoO=	M{O0b2Rqe3CuYXPlcYVvfCCxZjDbN2he[3mlbH;wZY1qdmViZoLvcUBUdW9iRES3N2ghdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHHmeIVzKDJiaILzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqKD1iMD6yN\|Ih\|ryPLh?=	NEH0NIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2N\|UzOid-MkOwOlM2OjJ:L3G+
CHO	MYDGeY5kfGmxbjDhd5NigQ>?			MVrBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHNudyC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KFt\|SG3IbE1C\yCkaX7kbY5oKGG\|c3H5MEBKSzVyIE2gNE4zQCEQvF2u	M37vOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmxOVU6Lz5zOUC5NVU2QTxxYU6=
Shh Light2	MXTGeY5kfGmxbjDhd5NigQ>?		MXW0NEBpenN?	NWeyS29RUW6qaXLpeIlwdiCxZjDTTGgheGG2aIfhfUBqdiCvb4Xz[UBUcGhiTHnnbJQzKGOnbHzzJIFnfGW{IESwJIhzeyCkeTDHcIku\GWyZX7k[Y51KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuN{DPxE1w	NWnafG9{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVI4QDhpPkKxOVkzPzh6PD;hQi=>
Shh-light2	NXT2dIZFTnWwY4Tpc44h[XO\|YYm=			MkSxTY5pcWKrdHnvckBw\iCVbX:tcYVlcWG2ZXSgTIghe2mpbnHsbY5oKGmwIHj1cYFvKFOqaD3sbYdpfDJiY3XscJMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBKSzVyIE2gNE4{KM7:TT6=	NEHMUIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2PFU2OSd-MkKyOlg2PTF:L3G+
M210B4	NWrsbGF7TnWwY4Tpc44h[XO\|YYm=		NE\3eHUzPCCqcoO=	M2ThdmlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJI1wfXOnIF2yNVBDPCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCSdHPoJI1TVkFiZYjwdoV{e2mxbjDh[pRmeiB{NDDodpMh[nliUmStVGNTKGGwYXz5d4l{NCCLQ{WwJF0hOC52MzFOwG0v	NWjWPJBxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4OTZpPkK0PVAxPzF4PD;hQi=>
Shh-Light 2	MkPTSpVv[3Srb36gZZN{[Xl?		NGHNWGEzKGSjeYO=	M{S1dWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiU33vJIlvKG2xdYPlJHNpcC2OaXfoeEAzKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gV4hpNWmwZIXj[YQhT2yrMT3y[ZBwenSncjDhZ5Rqfmm2eTDh[pRmeiB{IHThfZMh[nliZIXhcE1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgcYV1cG:mLDDJR\|UxKD1iMD60PFQh\|ryPLh?=	MoDmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ\|ME[zOVIzRC:jPh?=
Shh Light2	M3jZO2Z2dmO2aX;uJIF{e2G7			MlHaTY5pcWKrdHnvckBw\iCVSFigbY4hdW:3c3WgV4hpKEyrZ3j0NkBk\WyuczDifUBIVElvcnXzdI9ve2m4ZTDmbZJm\my7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvPSEQvF2u	NFrYNG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOwPVA5OCd-MUmzNFkxQDB:L3G+
C3H10T1/2	NYDMVIJJTnWwY4Tpc44h[XO\|YYm=			M2LESGlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDTTGgh[WO2aY\heIVlKHCjdHj3ZZkhcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W\|c3XkJIF{KFODRz3pcoR2[2WmIHPlcIwh\GmoZnXy[Y51cWG2aX;uJIJ6KGGua3HsbY5mKHCqb4PwbIF1[XOnIHHzd4F6NCCLQ{WwJF0hOC54IN88UU4>	NWOzbYg3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVI4QDhpPkKxOVkzPzh6PD;hQi=>
C3H10T1/2	MoTxSpVv[3Srb36gZZN{[Xl?		MX62JIhzew>?	NVjQUG0yUW6qaXLpeIlwdiCxZjDTRWcucW6mdXPl[EBlcW[oZYLlcpRq[XSrb36gc4YhdW:3c3WgcYV{\W6laIntZYwheGy3cnnwc5RmdnRiQ{PINVBVOS9{IHPlcIx{KHSxIHHsb4FtcW6nIIDoc5NxcGG2YYPlJJBwe2m2aY\lJI9me2WxYnzhd5R{KGGodHXyJFYhcHK\|LDDJR\|UxKD1iMD62NkDPxE1w	NI\JUog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2PFU2OSd-MkKyOlg2PTF:L3G+
ASZ001	M4PpTWZ2dmO2aX;uJIF{e2G7		NWjDVVlMPDhiaILz	MmnMTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC\|aXfuZYxqdmdiaX6gcY92e2ViQWPaNFAyKGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjZ4IN88UU4>	MnfyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO\|E3RC:jPh?=
M210B4	MWXGeY5kfGmxbjDhd5NigQ>?		MUWyOEBpenN?	NWf4OWV7UW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegbY4hdW:3c3WgUVIyOEJ2IHPlcIx{KGG\|c3Xzd4VlKGG\|IHTve45z\We3bHH0bY9vKG:oIFfsbVEhdVKQQTDlfJBz\XO\|aX;uJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNuIFnDOVAhRSByLkig{txONg>?	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEexOkc,OjR7MEC3NVY9N2F-
medulloblastoma cells	M{nTN2FvfGmycn;sbYZmemG2aY\lJIF{e2G7			M4PT[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUAyKM7:TT6=	MoXhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N\|EoRjF5NEG3OlMyRC:jPh?=
CHO	MUPGeY5kfGmxbjDhd5NigQ>?			NHjiTZpCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IH3veZNmKFOvbzDy[YNmeHSxcjDlfJBz\XO\|ZXSgbY4hS0iRIHPlcIx{KGK7IGuzTH1JcC2DZzDibY5lcW6pIHHzd4F6NCCLQ{WwJF0hOS5{IN88UU4>	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB7MUW1PUc,OTlyOUG1OVk9N2F-
Shh-Light2	M3LhNWZ2dmO2aX;uJIF{e2G7		MnK5OFghcHK\|	MojQTY5pcWKrdHnvckBw\iCVSFigbY4hdW:3c3WgV4hpNUyrZ3j0NkBk\WyuczDh[pRmeiB2ODDodpMh[nliR3zpNUBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJHNCTyxiSVO1NEA:KDFwM{GyJO69VS5?	NIXhN\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW0NVQ6OCd-MUm1OFE1QTB:L3G+
MEF	NEnGNFdHfW6ldHnvckBie3OjeR?=			NELDeHlKdmirYnn0bY9vKG:oIGPtc{BqdiCvb4Xz[UBRfGOqLXTl[olkcWWwdDDNSWYh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDTbIgucW6mdXPl[EBIdGlzIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSxiSVO1NEA:KDFwNTFOwG0v	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
MEF	M4X3VGZ2dmO2aX;uJIF{e2G7		M1LOZlMxKGi{cx?=	M376U2lvcGmkaYTpc44hd2ZiU33vJIlvKG2xdYPlJHB1[2hvZHXmbYNq\W62IF3FSkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGPobE1qdmS3Y3XkJGdtcS2{ZYPwc45{cX[nIHLleIFo[WyjY4Tvd4ll[XOnIHHjeIl3cXS7IHHmeIVzKDNyIHjyd{BjgSCEZYThS2xwKGG\|c3H5MEBKSzVyIE2gNU46KM7:TT6=	NFPNd489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3OFU1OSd-MkOwO\|Q2PDF:L3G+
neural precursor cells	NXHMSWFUSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=			M4T4dGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibnX1doFtKHC{ZXP1dpNweiClZXzsd{BjgSClb3zvcpkh\m:{bXH0bY9vKGG\|c3H5MEBGSzVyIE2gNVMvPDRizszNMi=>	NXPVd5A5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
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DU145	NXPSVI9rT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	NWDWV2t2PSC3TR?=	MYe5OkBpenN?	NIDISGhIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCjdDC1JJVOKGGodHXyJFk3KGi{cx?=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2OUGyOUc,OTh{NEmxNlU9N2F-
DU145	NHTHVmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NH\xXZYyOCC3TR?=	MorNPVYhcHK\|	MVfHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDEWVE1PSClZXzsd{BifCBzMDD1UUBi\nSncjC5OkBpenN?	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2OUGyOUc,OTh{NEmxNlU9N2F-
HEK293	MWLGeY5kfGmxbjDhd5NigQ>?			M1TSPWlvcGmkaYTpc44hd2ZiYnX0ZUBo[WyjY4Tvd4ll[XOnIHnuJGhGUzJ7MzDj[Yxtew>?	MkK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OUS3OlYoRjF5NEm0O\|Y3RC:jPh?=
22Rv	MmHMSpVv[3Srb36gZZN{[Xl?			M13Sd3Jm\HWldHnvckBw\iCneIDy[ZN{cW:wIH;mJHBVS0hibWLORUBqdiCqdX3hckAzOlK4IHPlcIx{	MlLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OUS3OlYoRjF5NEm0O\|Y3RC:jPh?=
PANC1	MlO0SpVv[3Srb36gZZN{[Xl?	MWqwMlIhfU1?	MXmyOEBpenN?	M17R[GlvcGmkaYTpc44hd2ZiSHivS2xKOS2vZXTpZZRm\CCSVFPIJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDQRW5EOSClZXzsd{BifCByLkKgeW0h[W[2ZYKgNlQhcHK\|IHL5JHJVNVCFUh?=	M1Hzd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWwNVcxLz5{MES1NFE4ODxxYU6=
PANC1	MW\GeY5kfGmxbjDhd5NigQ>?	MXGwMlQhfU1?	NHLzdoszPCCqcoO=	MX\Jcohq[mm2aX;uJI9nKEiqL1fMTVEudWWmaXH0[YQhWFSFSDDtVm5CKGW6cILld5Nqd25iaX6gbJVu[W5iUFHOR\|Eh[2WubIOgZZQhOC52IIXNJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1J?	MlLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUCxO\|AoRjJyNEWwNVcxRC:jPh?=
Shh Light2	NUXqVI9oTnWwY4Tpc44h[XO\|YYm=	Ml20Ok4zPSC3TR?=	NGL1cIg{OCCqcoO=	M3XLRmlvcGmkaYTpc44hd2ZiTj3wZYxucXSxeXzheIVlKFOqaDDpckBud3W\|ZTDTbIghVGmpaISyJINmdGy\|IHH0JFYvOjVidV2gZYZ1\XJiM{CgbJJ{KGK7IH\pdoVndHlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	NWP5[3psRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxOVE4OzFpPkG5NVUyPzNzPD;hQi=>
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HEK293	NWTlXmsxTnWwY4Tpc44h[XO\|YYm=	NVzFO2FWPSC3TR?=	MXixNEBpenN?	NETqS3pFcXOybHHj[Y1mdnRib3[gRm9FUVC\LXP5Z4xweGGvaX7lJIZzd21iaIXtZY4hW22xIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYSgOUB2VSCvZXHzeZJm\CCjZoTldkAyOCCqcoOgZpkhTEGSSTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgcYlkem:\|Y3;wbYMh[XO\|YYm=	MoD2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5M{[wOlMoRjJ5N{O2NFY{RC:jPh?=
HEK293	NWCzepdxTnWwY4Tpc44h[XO\|YYm=	M{LpWlUhfU1?	NVvucoFiOTBiaILz	MmnESIl{eGyjY3Xt[Y51KG:oIFLPSGlRYS2leXPsc5BidWmwZTDmdo9uKGi3bXHuJHNudyCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG2IEWgeW0hdWWjc4Xy[YQh[W[2ZYKgNVAhcHK\|IHL5JGZCS1NiYX7hcJl{cXN?	M4Gxc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{O2NFY{Lz5{N{ezOlA3OzxxYU6=
C3H10T1/2	NELrZW1HfW6ldHnvckBie3OjeR?=	MXi1OU42KHSxIES1NFAhdk1?		NFjJSWJEd22yZYTpeIl3\SCrbnjpZol1cW:wIH;mJHNudyCrbjDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBUSUdvaX7keYNm\CCJbHmxJJRz[W6\|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDheEA2PS53IITvJFQ2ODBibl2gZpkheVCFUjDhcoFtgXOrcx?=	NX6w[\|hmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO\|Q2PDFpPkKzNFc1PTRzPD;hQi=>
U2OS	NXvzZ5JrTnWwY4Tpc44h[XO\|YYm=	MU[1JJVO	NGOwcnA3KGi{cx?=	MVfBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGOqaX3ldolkKFOvbzC2N\|MhdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHPv[ZhxemW\|c3nu[{Bj\XSjIHHydoV{fGmwMj3HSnAh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBqdnS{YXPlcIx2dGG{IHLleIEh[XK{ZYP0bY4zNUeIUDDh[4dz\WejdHWg[o9zdWG2aX;uJIF1KDVidV2gZYZ1\XJiNjDodpMh[nliY3;u[o9k[WxibXnjdo9{[2:yeR?=	NWDJS5lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlM2OjJpPkKzNFY{PTJ{PD;hQi=>

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Snail / E-cadherin / Slug / Vimentin ; Gli1 / TGF-β1 / CXCR4 ; NF-κB / Cyclin D1 / MMP2 / MMP9	26859575 26137001 23599805
Immunofluorescence	PCNA / β-catenin ; Vimentin	28747625 24553082

In vivo

Administration of Cyclopamine at dose of 50 mg/kg/day for 22 days eradicates the HUCCT1 xenografts in mice with no obvious adverse effects. ^[2] Cyclopamine treatment at dose of 1.2 mg for 7 days induces significant apoptosis of tumor cells and decreases the tumor mass by 50-60% in Panc 05.04- and L3.6sl-derived tumors, respectively, but not in the BxPC3-SMO^low tumors. [3] Administration of Cyclopamine alone profoundly inhibits tumor metastases in xenografts of E3LZ10.7 and L3.6pl, and completely abrogates metastases when in combination of gemcitabine. ^[4]

Protocol (from reference)

Kinase Assay:^[1]	Hedgehog cell assay: This assay measures the end stage of the Hh signaling pathway, that is, the transcriptional modulation of Gli, using Luciferase as readout (Gli-Luc assay). Cyclopamine is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are resuspended in F12 Ham's/DMEM (1:1) containing 5% FBS and 15 mM Hepes pH 7.3, added to assay plates and incubated with Cyclopamine for approximately 30 minutes at 37 °C in 5% CO₂. 1 nM Hh-Ag 1.5 is then added to assay plates and incubated at 37 °C in the presence of 5% CO₂. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 value, defined as the inflection point of the logistic curve, is determined by non-linear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of Cyclopamine using the R statistical software pack
Cell Research:^[2]	Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, and HCT116 Concentrations: Dissolved in DMSO, final concentration 3 μM Incubation Time: 4 days Method: Cells are exposed to Cyclopamine in 96-well plates. Cell viability is measured by MTS (soluble tetrazolium salt) assay. Viable cell mass is determined by optical density measurements at 490 nm (OD₄₉₀) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)﹣OD (day 2)/OD (day 2). (Only for Reference)
Animal Research:^[2]	Animal Models: Athymic (nude) mice inoculated subcutaneously with HUCCT1 cells Dosages: 50 mg/kg/day Administration: Subcutaneous injection (Only for Reference)

References

[4] Feldmann G, et al. Cancer Res, 2007, 67(5), 2187-2196.

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Solubility (25°C)

In vitro	DMF	10 mg/mL warmed (24.29 mM)
	Ethanol	2 mg/mL warmed (4.85 mM)
	DMSO	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	411.62
Formula	C₂₇H₄₁NO₂
CAS No.	4449-51-8
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5CC4=C3C)C)O)C)NC1

Download Cyclopamine SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo use in mice?

Answer:
One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

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