Home Stem Cells & Wnt Hedgehog/Smoothened antagonist Cyclopamine

Cyclopamine

Catalog No.S1146 Synonyms: 11-deoxojervine

For research use only.

Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.

Cyclopamine Chemical Structure

CAS No. 4449-51-8

Selleck's Cyclopamine has been cited by 110 publications

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Hedgehog/Smoothened Signaling Pathways

Hedgehog/Smoothened Signaling Pathway Map

Biological Activity

Description Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signaling pathway antagonist of Smoothened (Smo) with IC50 of 46 nM in TM3Hh12 cells.
Targets
Smoothened [1]
(TM3Hh12 cells)
46 nM
In vitro

Cyclopamine inhibits the Hedgehog signaling pathway with an IC50 of 46 nM, and blocks the activity of human Smo receptor expressed in CHO-K1 cells in [3H]Hh-Ag binding assay with an IC50 of 280 nM. [1] Cyclopamine significantly inhibits Hedgehog pathway activity in a dose-dependent manner in gut-derived tumor cell lines expressing Patched (PTCH) mRNA, and induces growth inhibition of those tumor cell lines by 75-95% at the concentration of 3 μM, but ineffective towards the colon tumor cells without PTCH mRNA expression, suggesting the effects of Cyclopamine treatment are Hedgehog pathway related rather than generally cytotoxic. [2] By blocking Hedgehog signaling through direct interaction with Smo, Cyclopamine (10 μM) inhibits the proliferation of SMOhigh Cyclopamine-responsive cell lines L3.6sl and Panc 05.04 by 75-80%, and increases the apoptosis by 2.5- to 3.5-fold, without affecting the BxPC3-SMOlow cell line. [3] Cyclopamine treatment significantly decreases of Snail mRNA and increasea E-cadherin transcripts in the E3LZ10.7 cell line. Independent of inhibition of cell growth, Cyclopamine treatment significantly inhibits the invasive phenotype of Hedgehog-dependent L3.6pl cells, causing a >500-fold reduction in the number of transmigrating cells, but not that of the Hedgehog-independent cell line Panc-1. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
OS-RC-2 M{jmbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;lTWM2OD13Lki2OlYh|ryP NIThdIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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no-10 NFzSSnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTlwOUCzPUDPxE1? M4nxXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LS-513 M1;JSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPsTWM2OD1zMT6zOVQ4KM7:TR?= NV\YcpZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ALL-PO M33Mdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTFzLke3N|Qh|ryP MnTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
8-MG-BA NGnBe3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjITWZKSzVyPUGzMlEyOjNizszN M4\NWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
RPMI-8402 M4H1TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfoVVVCUUN3ME2xOU45PTN5IN88US=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
EoL-1-cell NIDpS|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fNW2lEPTB;MUiuOVk1QCEQvF2= NHjxXog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NALM-6 M{T1eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTvepJiUUN3ME2xPU4xOTZ5IN88US=> NGHaOVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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L-363 Ml3VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\zbHdYUUN3ME2zNU44PDZzIN88US=> NUHDco13RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LAMA-84 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTxUpc1UUN3ME2zNk42OjFzIN88US=> NWXRVIttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NOS-1 MlrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3W3b2lEPTB;M{SuNlk2PiEQvF2= NGWzcJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
BB30-HNC NFn4PVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTN2LkOzNFYh|ryP NYTHbnNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
BC-1 NEG3cWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTN5Lkm3OFYh|ryP MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
IST-SL2 M1zHXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLCfnFKSzVyPUO4MlIzPCEQvF2= NFzVVnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
D-392MG NYj1fVF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTRyLkKyNVUh|ryP MmDFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
no-11 MnrDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTRyLkW1NlEh|ryP M1Xxd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LC4-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjlT2dpUUN3ME20NE45PzF4IN88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
A388 NEjtSllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFOzOYdKSzVyPUSyMlU5PDhizszN NYC4TnNZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NTERA-S-cl-D1 M4nDNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTR{LkewO|Qh|ryP M2n5dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
CESS NYXnNmVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPPUpFDUUN3ME20OE4zOjN{IN88US=> M3PnTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
RS4-11 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXO4UWVnUUN3ME20PU4xQTN6IN88US=> NEjkfok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
MS-1 M3u0UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrLXJBKSzVyPUWwMlk{PTFizszN MkPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CTV-1 MoH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILOOZVKSzVyPUWxMlA4PCEQvF2= M4PXV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
D-502MG NVHSXWFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXWbVFlUUN3ME21NU43OjdzIN88US=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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SK-NEP-1 Mk\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTV|LkO5NlMh|ryP NVLoVYtXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LOXIMVI NHLDS4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37vVGlEPTB;NUOuOVg5PCEQvF2= M3;3WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DJM-1 NFK3b|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn[3TWM2OD13Nj6zN|kyKM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
GI-1 Mn;rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTDRWdIUUN3ME21Ok43OTR7IN88US=> MonOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
IST-MES1 M2TxeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HXOmlEPTB;NkCuOVQ6OyEQvF2= NXjQNYlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MV-4-11 NIH3SmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjxSldKSzVyPU[wMlY2OzhizszN M4f1XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
OVCAR-4 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3jTWM2OD14Mz61OlU4KM7:TR?= NH7VNIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KE-37 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvacItKSzVyPU[2MlI3PjhizszN MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
D-542MG MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTxbHlKSzVyPU[4MlQyOzVizszN M3THVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MHH-PREB-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjJe|FLUUN3ME23Nk45PDRzIN88US=> M4DtflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MRK-nu-1 NHq5dVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTd|LkS3NFUh|ryP MnfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-247MG M2DKN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rpZ2lEPTB;N{OuOVQ1OiEQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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LB2241-RCC NILlPI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHmxfmZKSzVyPUGxOk43PzlizszN NFjiVWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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NCI-H1395 NHnGdJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTF5OT65PVYh|ryP NHv5Spg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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NCI-H2107 MoPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTF7Mz63N|kh|ryP Mm[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
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ES4 NEPoZYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvsR3N3UUN3ME2yNlMvPzh|IN88US=> NHHC[lA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KARPAS-422 NYTvfVVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTJ{OD6zOVIh|ryP MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1648 NXHFcYQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PleWlEPTB;MkK5MlQ5QSEQvF2= M3\BbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES6 NYTwcVJyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTJ|OT60N{DPxE1? M2rsZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KNS-81-FD NX7KVJB3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLhXopKSzVyPUK0NU4yQTdizszN M1jx[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
JAR MmnzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTJ3Nj6yNlUh|ryP MmnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NB1 M2PISmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{P4[2lEPTB;Mk[wMlUyPiEQvF2= M2jmS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
D-336MG Mo\ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPuTWM2OD1{NkCuOlk5KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BC-3 M{P3Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nQe2lEPTB;Mk[1MlE4QCEQvF2= MnHTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HCC2218 NVrtdWZxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzoTWM2OD1{Nk[uOFE2KM7:TR?= M1;heFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
TE-9 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3lTWM2OD1{Nk[uOlI4KM7:TR?= NEO1b3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
LB1047-RCC NVy4c5g5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTJ4Nj63OVMh|ryP MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CTB-1 NE[1fHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknpTWM2OD1{NkmuPVc{KM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB7 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTJ5MTFOwG0> NIPZ[G49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ST486 MljPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3P5RWlEPTB;Mke3MlQyOiEQvF2= M2LY[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HCC1187 M13nWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{GxPGlEPTB;MkiyMlgyOSEQvF2= M3vuOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-SNU-16 NUTnNmFKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\nXWlEPTB;Mki0MlI1QCEQvF2= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
COR-L279 MmPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fVNGlEPTB;MkmxMlU5PCEQvF2= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES8 NVyxb41oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPrTWM2OD1{OUSuNVgzKM7:TR?= NFHCfoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
U-698-M NUC5T4QxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjoTWM2OD1{OUiuNlQ{KM7:TR?= NV\UTHg1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HEL M{PGSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFexSHpKSzVyPUOwPU4yPDlizszN NIeydIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KINGS-1 M1v4UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlK4TWM2OD1|MUCuOlc1KM7:TR?= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KY821 M2LKeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnToTWM2OD1|M{[uOVk2KM7:TR?= M3THeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MZ1-PC NEHKeI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjlS3lCUUN3ME2zOFUvPjF6IN88US=> NYG1RoJnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LS-411N NFXtXZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTN3ND62OkDPxE1? MkPWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SIG-M5 MmjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ztbmlEPTB;M{W5Mlc5OiEQvF2= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HT MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfLTWM2OD1|NkeuO|EyKM7:TR?= MkXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HC-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTN4Nz63PFch|ryP MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1694 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\sTWM2OD1|N{KuPVM1KM7:TR?= NXjFOo5{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
BB65-RCC NV;KfpFIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTN5Nj6yOFUh|ryP NXnQUoc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HAL-01 Mn2yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVz4WJF1UUN3ME2zO|kvQDN6IN88US=> Mmn4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ARH-77 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjQPIhKSzVyPUO5OE4xODhizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MZ7-mel MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTN7Nz6yN|Mh|ryP NEHycIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
SIMA NWe4S|dUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fIOmlEPTB;NECzMlk{OyEQvF2= M{DFdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DG-75 NFm0WY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHjeFU{UUN3ME20NVUvPjl6IN88US=> M4Oyb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HUTU-80 NX;xeJhrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXq[YJiUUN3ME20NVkvOTh3IN88US=> MmfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KNS-42 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDJTWM2OD12MkWuPFE2KM7:TR?= NV\yc3dtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SH-4 M16yWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Xh[2lEPTB;NEK3MlU3PSEQvF2= NFfGTIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
L-540 MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;Bd45KSzVyPUSzNU4xOzFizszN MkjMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NB10 M2PZV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTR2MT6yN|Qh|ryP NV\aXIllRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ES1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTR3Mj63OVMh|ryP M1z6TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KMOE-2 NXjXSWVxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXOW3VKSzVyPUS1Ok44OTFizszN M{nEUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MC116 NYHzbpRrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTR3OD6xNVYh|ryP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RCC10RGB M1PqXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\OSVVKSzVyPUS2NE4xODVizszN Mn3mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RL95-2 NWXZTIRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLBW3EzUUN3ME20OlAvOjN5IN88US=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
Raji NGLNeVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjlSYl7UUN3ME20OlgvOTR|IN88US=> NHfXWoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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DU145 MljQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGLnPIo2KHWP MUO5OkBpenN? NE\CWJJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCjdDC1JJVOKGGodHXyJFk3KGi{cx?= NEDDNWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0PVEzPSd-MUiyOFkyOjV:L3G+
DU145 NELOVW5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWmxNEB2VQ>? M4LCTlk3KGi{cx?= MkXNS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[XRiMUCgeW0h[W[2ZYKgPVYhcHK| NE\HVpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0PVEzPSd-MUiyOFkyOjV:L3G+
HEK293 MUPGeY5kfGmxbjDhd5NigQ>? NVT1R2FwUW6qaXLpeIlwdiCxZjDi[ZRiKGejbHHjeI9{cWSjc3WgbY4hUEWNMkmzJINmdGy| NViyWII1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0PVQ4PjZpPkG3OFk1PzZ4PD;hQi=>
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PANC1 NIjvcHlHfW6ldHnvckBie3OjeR?= NH\yZooxNjJidV2= M{fIbVI1KGi{cx?= M1PEbWlvcGmkaYTpc44hd2ZiSHivS2xKOS2vZXTpZZRm\CCSVFPIJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDQRW5EOSClZXzsd{BifCByLkKgeW0h[W[2ZYKgNlQhcHK|IHL5JHJVNVCFUh?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MEG3NEc,OjB2NUCxO|A9N2F-
PANC1 MoG3SpVv[3Srb36gZZN{[Xl? NHHINGQxNjRidV2= MlnJNlQhcHK| NETTSVZKdmirYnn0bY9vKG:oIFjoM2dNUTFvbXXkbYF1\WRiUGTDTEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gVGFPSzFiY3XscJMh[XRiMD60JJVOKGGodHXyJFI1KGi{czDifUBTXC2SQ2K= Mn;UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUCxO|AoRjJyNEWwNVcxRC:jPh?=
Shh Light2 M1zVeGZ2dmO2aX;uJIF{e2G7 NX7KTGk3Pi5{NTD1US=> MnjwN|AhcHK| MVLJcohq[mm2aX;uJI9nKE5vcHHscYl1d3muYYTl[EBUcGhiaX6gcY92e2ViU3joJGxq\2i2MjDj[YxteyCjdDC2MlI2KHWPIHHmeIVzKDNyIHjyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?= M3HLcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUWxO|MyLz5zOUG1NVc{OTxxYU6=
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HEK293 MlHoSpVv[3Srb36gZZN{[Xl? MmXsOUB2VQ>? NXHKXJltOTBiaILz MYLEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XRiNTD1UUBu\WG|dYLl[EBi\nSncjCxNEBpenNiYomgSGFRUSC|dHHpcolv\yCkYYPl[EBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yaXOgZZN{[Xl? Mn;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5M{[wOlMoRjJ5N{O2NFY{RC:jPh?=
HEK293 M1z2XWZ2dmO2aX;uJIF{e2G7 NEXmRYk2KHWP NX[yOI9zOTBiaILz M2i3U2Rqe3CuYXPlcYVvfCCxZjDCU2RKWFlvY4njcI9x[W2rbnWg[pJwdSCqdX3hckBUdW9iZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjdDC1JJVOKG2nYYP1doVlKGGodHXyJFExKGi{czDifUBHSUOVIHHuZYx6e2m| NHTTfoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ezOlA3Oyd-Mke3N|YxPjN:L3G+
C3H10T1/2 MkPlSpVv[3Srb36gZZN{[Xl? MorsOVUvPSC2bzC0OVAxKG6P MlzjR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDTcY8hcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV2FINWmwZIXj[YQhT2yrMTD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgOVUvPSC2bzC0OVAxKG6PIHL5JJFRS1JiYX7hcJl{cXN? M1:4clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEe0OVQyLz5{M{C3OFU1OTxxYU6=
U2OS MWDGeY5kfGmxbjDhd5NigQ>? NVn4WmpxPSC3TR?= MYe2JIhzew>? M2nsemFvfGGpb37pd5Qh[WO2aY\peJkh[XRiY3jpcYVzcWNiU33vJFY{OyCvdYThcpQh\XiycnXzd4VlKGmwIGWyU3Mh[2WubIOgZ49mgHC{ZYPzbY5oKGKndHGgZZJz\XO2aX6yMWdHWCCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGmwdILhZ4VtdHWuYYKgZoV1[SCjcoLld5RqdjJvR1\QJIFo\3KnZ3H0[UBnd3KvYYTpc44h[XRiNTD1UUBi\nSncjC2JIhzeyCkeTDjc45nd2OjbDDtbYNzd3Olb4D5 MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot Snail / E-cadherin / Slug / Vimentin ; Gli1 / TGF-β1 / CXCR4 ; NF-κB / Cyclin D1 / MMP2 / MMP9 26859575 26137001 23599805
Immunofluorescence PCNA / β-catenin ; Vimentin 28747625 24553082
In vivo Administration of Cyclopamine at dose of 50 mg/kg/day for 22 days eradicates the HUCCT1 xenografts in mice with no obvious adverse effects. [2] Cyclopamine treatment at dose of 1.2 mg for 7 days induces significant apoptosis of tumor cells and decreases the tumor mass by 50-60% in Panc 05.04- and L3.6sl-derived tumors, respectively, but not in the BxPC3-SMOlow tumors. [3] Administration of Cyclopamine alone profoundly inhibits tumor metastases in xenografts of E3LZ10.7 and L3.6pl, and completely abrogates metastases when in combination of gemcitabine. [4]

Protocol (from reference)

Kinase Assay:[1]
  • Hedgehog cell assay:

    This assay measures the end stage of the Hh signaling pathway, that is, the transcriptional modulation of Gli, using Luciferase as readout (Gli-Luc assay). Cyclopamine is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are resuspended in F12 Ham's/DMEM (1:1) containing 5% FBS and 15 mM Hepes pH 7.3, added to assay plates and incubated with Cyclopamine for approximately 30 minutes at 37 °C in 5% CO2. 1 nM Hh-Ag 1.5 is then added to assay plates and incubated at 37 °C in the presence of 5% CO2. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 value, defined as the inflection point of the logistic curve, is determined by non-linear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of Cyclopamine using the R statistical software pack
Cell Research:[2]
  • Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, and HCT116
  • Concentrations: Dissolved in DMSO, final concentration 3 μM
  • Incubation Time: 4 days
  • Method: Cells are exposed to Cyclopamine in 96-well plates. Cell viability is measured by MTS (soluble tetrazolium salt) assay. Viable cell mass is determined by optical density measurements at 490 nm (OD490) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)﹣OD (day 2)/OD (day 2).
Animal Research:[2]
  • Animal Models: Athymic (nude) mice inoculated subcutaneously with HUCCT1 cells
  • Dosages: 50 mg/kg/day
  • Administration: Subcutaneous injection

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 411.62
Formula

C27H41NO2
CAS No. 4449-51-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5CC4=C3C)C)O)C)NC1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

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% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to reconstitute the compound for in vivo use in mice?

Answer:
One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

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