Ciliobrevin D

For research use only.

Catalog No.S9743

Ciliobrevin D Chemical Structure

CAS No. 1370554-01-0

Ciliobrevin D (compound 5) is a cell-permeable, reversible and specific antagonist of AAA+ (ATPases associated with diverse cellular activities) ATPase motor cytoplasmic dynein. Ciliobrevin D perturbs primary cilia formation and blocks Hedgehog (Hh) signaling.

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Biological Activity

Description Ciliobrevin D (compound 5) is a cell-permeable, reversible and specific antagonist of AAA+ (ATPases associated with diverse cellular activities) ATPase motor cytoplasmic dynein. Ciliobrevin D perturbs primary cilia formation and blocks Hedgehog (Hh) signaling.
Targets
cytoplasmic dynein [1]
()
Hedgehog [1]
()
In vitro

Ciliobrevin D inactivate dynein in Sertoli cells. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the Sertoli cells in vitro.[2] Ciliobrevin D perturbs protein trafficking within the primary cilium, leading to their malformation and Hedgehog signaling blockade. Ciliobrevin D also prevents spindle pole focusing, kinetochore-microtubule attachment, melanosome aggregation, and peroxisome motility in cultured cells.[1]

In vivo

Ciliobrevin D inactivate dynein in the testis. The inactivation of dynein by ciliobrevin D also perturbs gross disruption of F-actin across the seminiferous epithelium in vivo, illustrating there are cross talks between the two cytoskeletons in the testis.[2]

Protocol

Cell Research:

[2]

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  • Cell lines: Sertoli cells
  • Concentrations: 15 µM, 30 µM
  • Incubation Time: 1 h
  • Method:

    Sertoli cells cultured in vitro on Matrigel-coated bicameral units at 1.0 × 106 cells/cm2 are used for quantifying the transepithelial electrical resistance in ohms (Ω) across the cell epithelium to assess the TJ-barrier function. In brief, each bicameral unit is placed inside the well of a 24-well dish with 0.5 ml F12/DMEM in the apical and the basal compartments. Dynein is inhibited in Sertoli cells on days 3 and 4 using Ciliobrevin D at 15 µM or 30 µM for 1hr, and Sertoli cell TJ-permeability barrier function is monitored daily by quantifying transepithelial electrical resistance across the cell epithelium.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: adult male Sprague-Dawley rats
  • Dosages: 15 µM per testis
  • Administration: local injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 20 mg/mL (50.93 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 392.62
Formula

C17H8Cl3N3O2

CAS No. 1370554-01-0
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID