Jervine

Catalog No.S4747 Synonyms: 11-Ketocyclopamine

For research use only.

Jervine (11-Ketocyclopamine) is a Hedgehog signaling (IC50=500-700 nM) inhibitor that inhibits the sonic hedgehog (shh) pathway by interacting with smoothened.

Jervine Chemical Structure

CAS No. 469-59-0

Selleck's Jervine has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description Jervine (11-Ketocyclopamine) is a Hedgehog signaling (IC50=500-700 nM) inhibitor that inhibits the sonic hedgehog (shh) pathway by interacting with smoothened.
Targets
Hedgehog [1] Shh [1]
In vitro

Compared to controls, chondrocyte cultures exposed to 25 μg/ml jervine shows an apparent reduction in cell number but no differences in cellular morphology or relative intensity of alcian blue staining[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
s12 cells Ml\kSpVv[3Srb36gZZN{[Xl? MkfDTY5pcWKrdHnvckBw\iCuaYDp[E1ud2SrZnnl[EApd2O2eXzheIVlMSCob4LtJI9nKFOKSDDlfJBz\XO|ZXSgbY4hdW:3c3Wgd|EzKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkWg{txO NEfmZY4yQTNyOUC4NC=>
In vivo Embryos exposed to jervine develop craniofacial abnormalities related to those found in Shh mutant embryos. The lower jaw of these embryos is severely reduced in size, often leading to loss of the incisors. Treatment of pregnant mice with the hedgehog inhibitor jervine induces jaw defects in the embryos similar to those found in Prx1−/−Prx2−/− embryos. The distal part of the jaw is reduced in size, and both incisors are absent[1].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: C3H/10T 1/2 CL8 cells
  • Concentrations: 5, 10, or 25 μg/ml
  • Incubation Time: 48 h
  • Method:

    Cells are grown in Eagle's basal medium supplemented with 10% fetal bovine serum and 0.25 mg/ml gentamicin sulfate. Cells are seeded onto 35-mm tissue culture plates at a density of 1×104 celldplate. The plated cells are incubated for 24 hours prior to treatment in a humidified incubator at 37 "C in an atmosphere of 5% CO2 in air. Drugs are then added: jervine to a final concentration of 5, 10, or 25 μg/ml; retinoic acid at 0.03, 0.30, or 3.0 pg/ml. After a final 48 hours of incubation, media are removed, the cultures are rinsed three times with phosphate-buffered saline (PBS) pH 7.4, and the cells are harvested into 0.5 ml of 0.1% (w/v) trypsin which contained 0.1% EDTA (w/v) in PBS. Each sample is mixed with 9.5 ml Isoton Ⅱ, and cell numbers are determined by using a Coulter Counter.

Animal Research:

[1]

  • Animal Models: A/J mice
  • Dosages: 400 mg/kg
  • Administration: oral administration

Solubility (25°C)

In vitro

DMSO 5 mg/mL
(11.74 mM)
Water Insoluble
Ethanol '2 mg/mL

Chemical Information

Molecular Weight 425.60
Formula

C27H39NO3

CAS No. 469-59-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CC2C(C(C3(O2)CCC4C5CC=C6CC(CCC6(C5C(=O)C4=C3C)C)O)C)NC1

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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