JK184

Catalog No.S6565

For research use only.

JK184 inhibits Gli in the Hedgehog (Hh) pathway with IC50 of 30 nM in mammalian cells.

JK184 Chemical Structure

CAS No. 315703-52-7

Selleck's JK184 has been cited by 1 Publication

Purity & Quality Control

Choose Selective Hedgehog/Smoothened Inhibitors

Other Hedgehog/Smoothened Products

Biological Activity

Description JK184 inhibits Gli in the Hedgehog (Hh) pathway with IC50 of 30 nM in mammalian cells.
Targets
Gli [1]
In vitro

JK184 antagonizes Hh signaling by inhibiting Gli-dependent transcriptional activity in a dose dependent manner. JK184 has a stronger inhibiting effects in mammalian cells with the IC50 of 30 nM compared with cyclopamine (IC50 = 250 nM)[1].

In vivo

JK184 has a poor pharmacokinetic profile and bioavailability, because the half-life and Cmax of JK184 is lower compared with cyclopamine. JK184 micelles are a promising drug candidate for treating pancreatic tumors with high inhibitory effect on Hh activity[1].

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 350.44
Formula

C19H18N4OS

CAS No. 315703-52-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCOC1=CC=C(C=C1)NC2=NC(=CS2)C3=C(N=C4N3C=CC=C4)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy JK184 | JK184 supplier | purchase JK184 | JK184 cost | JK184 manufacturer | order JK184 | JK184 distributor