Catalog No.S6565

For research use only.

JK184 inhibits Gli in the Hedgehog (Hh) pathway with IC50 of 30 nM in mammalian cells.

JK184 Chemical Structure

CAS No. 315703-52-7

Selleck's JK184 has been cited by 1 Publication

Purity & Quality Control

Choose Selective Hedgehog/Smoothened Inhibitors

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Biological Activity

Description JK184 inhibits Gli in the Hedgehog (Hh) pathway with IC50 of 30 nM in mammalian cells.
Gli [1]
In vitro

JK184 antagonizes Hh signaling by inhibiting Gli-dependent transcriptional activity in a dose dependent manner. JK184 has a stronger inhibiting effects in mammalian cells with the IC50 of 30 nM compared with cyclopamine (IC50 = 250 nM)[1].

In vivo

JK184 has a poor pharmacokinetic profile and bioavailability, because the half-life and Cmax of JK184 is lower compared with cyclopamine. JK184 micelles are a promising drug candidate for treating pancreatic tumors with high inhibitory effect on Hh activity[1].

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 350.44


CAS No. 315703-52-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCOC1=CC=C(C=C1)NC2=NC(=CS2)C3=C(N=C4N3C=CC=C4)C

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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