GLI1 Antibody [A19H14]

Catalog No.: F0381

For research use only.

    Home Primary Antibodies GLI1 Antibody [A19H14]

    Application: Reactivity:

    Experiment Essentials

    WB
    Recommended SDS-PAGE separating gel concentration: 5%.

    Usage Information

    Dilution
    1:1000
    1:50
    Application
    WB, IP
    Reactivity
    Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW
    160 kDa
    Positive Control RMS-13 cell; TOV-112D cell
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity
    GLI1 Antibody [A19H14] detects endogenous levels of total GLI1 protein.
    Subcellular Location
    Cytoplasm, Nucleus
    Uniprot ID
    P08151
    Clone
    A19H14
    Synonym(s)
    Gli; hedgehog; sonic
    Background
    GLI1 (Glioma-associated oncogene homolog 1) is a zinc finger transcription factor and terminal effector of the Hedgehog (Hh) signaling pathway, originally identified as an amplified gene in malignant glioma. Structurally, GLI1 is a 1106 amino acid protein containing five tandem C2-H2 zinc finger DNA-binding domains that recognize the consensus sequence 5′-GACCACCCA-3′, a nuclear localization signal (NLS), nuclear export signal (NES), an N-terminal SUFU-interacting domain (SIN) that mediates cytoplasmic retention, and a C-terminal transactivation domain (TAD) that recruits chromatin remodelers and co-activators. GLI1 is expressed at low levels in differentiated tissues but is tightly regulated during embryonic development and tissue patterning, where it promotes cell differentiation and tissue polarity. Functionally, GLI1 acts as a strong transcriptional activator, regulating genes involved in cell cycle progression, DNA replication, and DNA damage repair, while its aberrant activation drives oncogenic processes including proliferation, survival, angiogenesis, metastasis, and chemoresistance in various cancers.
    References
    • https://pubmed.ncbi.nlm.nih.gov/34113570/
    • https://pubmed.ncbi.nlm.nih.gov/22391302/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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