Fluorouracil (5-Fluoracil, 5-FU)
Catalog No.S1209 Synonyms: NSC 19893
Molecular Weight(MW): 130.08
Fluorouracil (5-Fluoracil, 5-FU) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells.
Cited by 13 Publications
2 Customer Reviews
DNA-PKcs suppression mediated ROS production and GSH content in HepG2 cells exposed to CDDP and 5-Fu. a DNA-PKcs inhibition promoted ROS production in HepG2 cells treated with indicated concentrations of CDDP and 5-Fu. DCFH-DA fluorescent analysis was performed to assess the ROS level. Data presented were mean ?SD of three independent experiments.
Mol Cell Biochem 2014 10.1007/s11010-014-2253-6. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.
EdU staining of RBE cells treated with OSI-027 (6.25 μM) and/or 5-FU (6.25 μg/mL) was performed by using Click-iT EdU Imaging Kit. The percentages of EdU-positive cells have been provided in the right panel.
Eur Rev Med Pharmacol Sci, 2016, 20(9):1699-706.. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.
Purity & Quality Control
Choose Selective DNA/RNA Synthesis Inhibitors
|Description||Fluorouracil (5-Fluoracil, 5-FU) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells.|
Adrucil is an analogue of uracil with a fluorine atom at the C-5 position in place of hydrogen. It rapidly enters the cell using the same facilitated transport mechanism as uracil. Adrucil is converted intracellularly to several active metabolites: fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate (FUTP). The Adrucil metabolite FdUMP binds to the nucleotide-binding site of TS, forming a stable ternary complex with the enzyme and CH2THF, thereby blocking binding of the normal substrate dUMP and inhibiting dTMP synthesis. Metabolite of Adrucil also can be misincorporated into DNA, leading to DNA strand breaks and cell death. The pro-apoptosis effects of Adrucil may be related to its activation of tumor suppressor p53. Loss of p53 function reduces cellular sensitivity to Adrucil.  Adrucil is able to inhibit the survival and induce apoptosis of a board range of cancer cells. Adrucil suppresses viabilities of the nasopharyngeal carcinoma cell line CNE2 and HONE1 , pancreatic cancer cell lines Capan-1 , and human colon carcinoma cell line HT-29  with IC50 of 9 μg/mL, 3 μg/mL, 0.22 μM, 2.5 μM, respectively.
|In vivo||Adrucil is widely used in the treatment of a range of cancers, including colorectal and breast cancers.  100mg/kg Adrucil significantly suppresses tumor growth of murine colon carcinomas Colon 38 with tumor-doubling time (TD), growth-delay factor (GDF), and T/C of 26.5 days, 4.4, and 14%. |
-  Longley DB, et al. Nat Rev Cancer, 2003, 3(5), 330-338.
-  Qin L, et al. Biochem Biophys Res Commun, 2008, 371(3), 531-535.
-  Shi X, et al. Oncology, 2002, 62(4), 354-362.
|In vitro||DMSO||26 mg/mL (199.87 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03196180||Not yet recruiting||High Grade Cervical Intraepithelial Neoplasia|p16 Positive Neoplastic Cells Present||National Cancer Institute (NCI)||February 28 2019||Phase 1|
|NCT03607643||Not yet recruiting||Cancer of Pancreas|Cancer of Liver|Cancer of Rectum|Cancer of Colon|Cancer Gall Bladder|Myeloma Multiple|Glioblastoma Multiforme||Leaf Vertical Inc.||January 15 2019||Phase 1|Phase 2|
|NCT03698461||Not yet recruiting||Colorectal Neoplasms|Neoplasm Metastasis|Colonic Neoplasms|Rectal Neoplasms||Asan Medical Center|Hoffmann-La Roche||December 2018||Phase 2|
|NCT03203525||Not yet recruiting||Liver Cancer||M.D. Anderson Cancer Center|NovoCure Ltd.||December 2018||Phase 1|
|NCT03727074||Not yet recruiting||Actinic Keratosis (AK)||Sol-Gel Technologies Ltd.||December 2018||Phase 3|
|NCT03721653||Not yet recruiting||Metastatic Colorectal Cancer||Gruppo Oncologico del Nord-Ovest|Roche Pharma AG||November 15 2018||Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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Frequently Asked Questions
I was wondering if the product #s1209 (5-fluorouracil) is suitable to inject into mice ?
S1209 is suitable to inject (I.P.) into mice as indicating in this paper: http://www.ncbi.nlm.nih.gov/pubmed/8995503.