Name: Azaguanine-8
Brand: Selleck Chemicals
SKU: S4194
Availability: InStock
Rating: 5 (8 reviews)

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Quality Control

Batch: Purity: 99.82%

99.82

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN Topoisomerase PPAR Sirtuin Casein Kinase eIF
Other DNA/RNA Synthesis Inhibitors	CX-5461 (Pidnarulex) B02 SCR7 Favipiravir (T-705) EED226 BMH-21 RK-33 Triapine (3-AP) Carmofur YK-4-279

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
H.Ep cells	Cytotoxicity assay				Cytotoxicity against H.Ep cells, IC50=2 μM	6620307
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 4 mg/mL (26.29 mM) Warmed with 50°C water bath; Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 45%PEG300 2 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.000mg/ml (13.15mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 450 μL of PEG 300, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	152.11	Formula	C₄H₄N₆O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	134-58-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC-749, SF-337, SK 1150			Smiles	C12=NNN=C1N=C(NC2=O)N

Mechanism of Action

In vitro	8-Azaguanine is a purine analogue that shows antineoplastic activity. It functions as an antimetabolite and easily incorporates into ribonucleic acids, interfering with normal biosynthetic pathways, thus inhibiting cellular growth.
In vivo	A study of the in vivo metabolism of 8-azaguanine-2-C 14 by mouse neoplasms that are susceptible or resistant to growth inhibition by 8-azagnanine have been reported. The results show that the growth of those neoplasms which convert significant amounts of 8-azaguanine to 8-azaguanylic acid is inhibited by 8-azaguanine.
References	[1] https://pubmed.ncbi.nlm.nih.gov/17055708/ [2] https://pubmed.ncbi.nlm.nih.gov/13629483/

Applications

Methods	Biomarkers	Images	PMID
Western blot	Ac-H3		26668215
Growth inhibition assay	Cell viability		30804405

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Handling Instructions

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Azaguanine-8 DNA/RNA Synthesis chemical

Chemical Structure

Molecular Weight: 152.11