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Madrasin

Name: Madrasin
Brand: Selleck Chemicals
SKU: S0280
Rating: 5 (1 reviews)

Synonyms: DDD00107587

Catalog No.S0280 Purity: 99.97%

Madrasin (DDD00107587) is a potent and cell-penetrant pre-mRNA splicing inhibitor. Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex.

Madrasin Chemical Structure

CAS: 374913-63-0

Selleck's Madrasin has been cited by 1 publication

Purity & Quality Control

Batch: S028001 Ethanol] 3 mg/mL] false] DMSO] 1 mg/mL] false] Water] Insoluble] false Purity: 99.97%

99.97

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Signaling Pathway

DNA/RNA Synthesis Signaling Pathway

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description	Madrasin (DDD00107587) is a potent and cell-penetrant pre-mRNA splicing inhibitor. Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex.

In vitro
In vitro	Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex. Madrasin is cytotoxic at higher concentrations, although at lower concentrations it induces cell cycle arrest, promotes a specific reorganization of subnuclear protein localization, and modulates splicing of multiple pre-mRNAs in both HeLa and HEK293 cells.^[1]
Cell Research	Cell lines	HeLa cells, HEK293 cells
	Concentrations	10-30 μM
	Incubation Time	2-24 h
	Method	HeLa cells are seeded in 6-well plates, and after cells are treated with Madrasin. The cells are harvested after 4, 8, and 24 h and then washed twice with PBS, resuspended in ice-cold 70% ethanol, and fixed at room temperature for 30 min. Fixed cells are used for flow cytometry analysis.

References

[1] Andrea Pawellek, et al. J Biol Chem. 2014 Dec 12;289(50):34683-98.

Chemical Information & Solubility

Molecular Weight	311.34	Formula	C₁₆H₁₇N₅O₂
CAS No.	374913-63-0	SDF	--
Smiles	CC1=C(N=C(NC1=O)NC2=NC(=C3C=CC(=CC3=N2)OC)C)C
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

Ethanol : 3 mg/mL

DMSO : 1 mg/mL ( (3.21 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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