BMH-21

Catalog No.S7718

BMH-21 Chemical Structure

Molecular Weight(MW): 360.41

BMH-21 is a DNA intercalator, which binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription.

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Cited by 4 Publications

1 Customer Review

  • Pharmacological and siRNA perturbations in HEK293 cells suggest that mTORC1 also modulates cytoplasmic rheology through ribosome crowding in mammals.

    Cell, 2018, 174(2):338-349. BMH-21 purchased from Selleck.

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Biological Activity

Description BMH-21 is a DNA intercalator, which binds ribosomal DNA and inhibits RNA polymerase I (Pol I) transcription.
Targets
RNA polymerase I [1]
In vitro

BMH-21 causes proteasome-dependent destruction of RPA194, the large catalytic subunit protein of Pol I holocomplex. [1] In U2OS cancer cell line, BMH-21 results in degradation of RPA194 and translocation of NCL with IC50 of 0.05 μM and 0.07 μM, respectively. By causing nucleolar stress, BMH-21 also shows potent inhibition on cell viability. [2]

Assay
Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 28656213     


SKOV3 cells were treated with varying doses of BMH-21 for 24 h. Cell viability was determined by the MTT assay.

28656213
Western blot
Bax / Caspase-3 / Cleaved caspase-3 ; 

PubMed: 28656213     


SKOV3 cells were treated with varying doses of BMH-21 (1, 2 and 4 µM) for 24 h. Expression of BAX and cleaved caspase-3 were determined by western blotting.

MDM2 / p53 / p-p53 ; 

PubMed: 28656213     


SKOV3 cells were treated with varying doses of BMH-21 (1, 2 and 4 µM) for 24 h. Expression of MDM2, p53 and p-p53-Ser15 were determined by western blotting. 

28656213
Immunofluorescence
Fibrillarin; 

PubMed: 28656213     


SKOV3 cells were treated with varying doses of BMH-21 (1, 2 and 4 µM) for 24 h. The expression and cellular localization of fibrillarin was observed using confocal microscopy (scale bar, 5 µm).

Nuclephosmin; 

PubMed: 28656213     


SKOV3 cells were treated with varying doses of BMH-21 (1, 2 and 4 µM) for 24 h. The expression and cellular localization of nucleophosmin was observed using confocal microscopy (scale bar, 5 µm).

28656213
In vivo BMH-21 reduces tumor growth in mouse xenografts. [1]

Protocol

Cell Research:

[2]

- Collapse
  • Cell lines: U2OS osteosarcoma cells
  • Concentrations: ~5 μM
  • Incubation Time: 48 hours
  • Method:

    The cells are maintained at 37 °C in a humidified atmosphere containing 5% CO2. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h with the compounds. Viability is determined using WST-1 cell proliferation reagent.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 5 mg/mL warmed (13.87 mM)
Ethanol 2 mg/mL warmed (5.54 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 360.41
Formula

C21H20N4O2

CAS No. 896705-16-1
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID