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B02 DNA/RNA Synthesis inhibitor

Name: B02
Brand: Selleck Chemicals
SKU: S8434
Availability: InStock
Rating: 5 (20 reviews)

Cat.No.S8434

B02 is a small-molecule inhibitor of human RAD51 with an IC50 of 27.4 μM, but this compound does not inhibit its E. coli homologue RecA (IC50 > 250 μM).

Chemical Structure

Molecular Weight: 339.39

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S843401 DMSO]67 mg/mL]false]Ethanol]20 mg/mL]false]Water]Insoluble]false Purity: 99.93%

99.93

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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description
HEK cells	Function assay	25 uM		Inhibition of DNA binding to human RAD51 in irradiated HEK cells assessed as reduction in RAD51 foci formation at 25 uM by fluorescence microscopy relative to untreated control,
MEF cells	Function assay	5 uM	1 h	Inhibition of RAD51 in Tp53-/- MEF cells assessed as potentiation of 32 uM cisplatin-induced cytotoxicity at 5 uM incubated for 1 hr by clonogenic survival assay relative to untreated control
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	339.39	Formula	C₂₂H₁₇N₃O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1290541-46-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1=CC=C(C=C1)CN2C(=NC3=CC=CC=C3C2=O)C=CC4=CN=CC=C4

Solubility

In vitro
Batch:

DMSO : 67 mg/mL (197.41 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 20 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.300mg/ml (9.72mM)	Taking the 1 mL working solution as an example, add 50 μL of 66 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.600mg/ml (4.71mM)	Taking the 1 mL working solution as an example, add 50 μL of 32 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	RAD51 ^[2] (Cell-free assay) 27.4 μM
In vitro	B02 is a specific inhibitor of human RAD51 recombinase, blocks HR repair in human embryonic kidney (HEK) and breast cancer cells and increases their sensitivity to a wide range of DNA damaging agents. Also, this compound enhances DNA damage and apoptosis induced by decitabine in MM cells^[1]. It shows high specificity for RAD51 and does not significantly inhibit RAD54 in the range of concentrations from 0 to 200 μM^[2]. This chemical shows biological effect in human and mouse cells. In human embryonic kidney (HEK) cells, it disrupts RAD51 foci formation in response to DNA damage and inhibited DSB repair and DSB-dependent HR. It can also increase the sensitivity of cancer cells to chemotherapeutic DNA damaging agents^[3].
In vivo	B02 significantly increases the anti-tumor activity of cisplatin in vivo. This compound is tolerated by mice at doses up to 50 mg/kg without obvious body weight loss. No detectable morphological changes induced by this chemical in kidneys and livers, main organs for detoxification are found^[3].
References	[1] https://pubmed.ncbi.nlm.nih.gov/25401086/ [2] https://pubmed.ncbi.nlm.nih.gov/21428443/ [3] https://pubmed.ncbi.nlm.nih.gov/24971740/

Tech Support

Handling Instructions

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