Molecular Weight(MW): 339.39
B02 is a small-molecule inhibitor of human RAD51 with an IC50 of 27.4 μM, but does not inhibit its E. coli homologue RecA (IC50 > 250 μM).
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HEK 293T cells were transfected with a total of 6 μg of plasmid DNA (2 μg pSG-I-SceI-HA, 2 μg pcDNA-HBZ-Myc-His or empty vector, and 2 μg the pDR-GFP reporter) and treated with 20 μM B02 for 48 h where indicated. Data shown are averages of results from three independent experiments, and error bars represent SEM (*, P ≤ 0.05 by Student's t test).
J Virol, 2018, 92(15). B02 purchased from Selleck.
The number of RAD51 foci per nucleus were counted from at least 50 nuclei for each treatment. Following DMSO, RI-1 or B02 pre-treatment, cells were incubated in normal media or with 10 nM doxorubicin (Dox), 1 μM cisplatin (Cis), 0.1 nM vincristine (Vin) or 10 μM GDC-0152 (GDC) for 24 hours then harvested.
Sci Rep, 2018, 8(1):14421. B02 purchased from Selleck.
Purity & Quality Control
Choose Selective DNA/RNA Synthesis Inhibitors
|Description||B02 is a small-molecule inhibitor of human RAD51 with an IC50 of 27.4 μM, but does not inhibit its E. coli homologue RecA (IC50 > 250 μM).|
B02 is a specific inhibitor of human RAD51 recombinase, blocks HR repair in human embryonic kidney (HEK) and breast cancer cells and increases their sensitivity to a wide range of DNA damaging agents. Also, B02 enhances DNA damage and apoptosis induced by decitabine in MM cells. B02 shows high specificity for RAD51 and does not significantly inhibit RAD54 in the range of concentrations from 0 to 200 μM. B02 shows biological effect in human and mouse cells. In human embryonic kidney (HEK) cells, B02 disrupts RAD51 foci formation in response to DNA damage and inhibited DSB repair and DSB-dependent HR. B02 can also increase the sensitivity of cancer cells to chemotherapeutic DNA damaging agents.
|In vivo||B02 significantly increases the anti-tumor activity of cisplatin in vivo. B02 is tolerated by mice at doses up to 50 mg/kg without obvious body weight loss. No detectable morphological changes induced by B02 in kidneys and livers, main organs for detoxification are found.|
|In vitro||DMSO||67 mg/mL warmed (197.41 mM)|
|Ethanol||20 mg/mL warmed (58.92 mM)|
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