For research use only.

Catalog No.S8246

5 publications

RK-33 Chemical Structure

CAS No. 1070773-09-9

RK-33 is a first-in-class small molecule inhibitor of DDX3 (a RNA helicase) and causes G1 cell cycle arrest, induces apoptosis, and promotes radiation sensitization in DDX3-overexpressing cells.

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Biological Activity

Description RK-33 is a first-in-class small molecule inhibitor of DDX3 (a RNA helicase) and causes G1 cell cycle arrest, induces apoptosis, and promotes radiation sensitization in DDX3-overexpressing cells.
DDX3 [1]
In vitro

RK-33 binds specifically to DDX3, but not to the closely related proteins DDX5 and DDX17. RK-33 inhibits cancer growth and radiosensitizes lung cancer cells in a DDX3-dependent manner. RK-33 has no effect on either mitochondrial respiration or ATP generation. RK-33 curbs proliferation and induces apoptosis in a DDX3-dependent fashion. Wnt signaling is mediated by DDX3 and inhibited by RK-33. RK-33 impairs radiation-induced DNA damage repair by inhibiting NHEJ activity[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 cells NIfZN3dEgXSxdH;4bYNqfHliYYPzZZk> NEXvR|E4OiCq Mlu1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVOSCjc4PhfUwhUUN3ME2yMlUh|ryP M2DOVlIyPTd{NUSx
A549 cells NXHl[2hiS3m2b4TvfIlkcXS7IHHzd4F6 NF[1VG04OiCq M2rrRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHdUXDFiYYPzZZktKEmFNUC9Nk42PiEQvF2= M1L2b|I{OjlyMkWy
H460 cells MULDfZRwfG:6aXPpeJkh[XO|YYm= NF\kcWg4OiCq NF3ieXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBYW1RzIHHzd4F6NCCLQ{WwQVIvQCEQvF2= NFXONXAzOTV5MkW0NS=>
PC3 cells M1LlZmN6fG:2b4jpZ4l1gSCjc4PhfS=> NEPRTVc4OiCq NUL6V|d3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN|IHPlcIx{KGGodHXyJFczKGi{czDifUBYW1RzIHHzd4F6NCCLQ{WwQVQvQCEQvF2= NG\Eb4IzOTV5MkW0NS=>
MCF7 cells NWjKe3JWS3m2b4TvfIlkcXS7IHHzd4F6 NUXadVVLPzJiaB?= NH\oTodEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBYW1RzIHHzd4F6NCCLQ{WwQVcvPSEQvF2= MX:yNVU4OjV2MR?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot

PubMed: 30292066     

Immunoblots showing DDX3 expression levels in medulloblastoma cell lines after treatment with RK-33 for 48 h. 

Cyclin D1 / Cyclin E1 ; 

PubMed: 25820276     

Immunoblot of cell cycle-related proteins (Cyclin D1 and Cyclin E1) and cell death-related proteins (cleaved caspase 7, cleaved caspase 9) in H1299 cells after treatment with RK-33 (10 μM).

30292066 25820276
Growth inhibition assay
Cell viability; 

PubMed: 27634756     

Cell viability assay of RK-33 on PC3, DU145, 22Rv1, and LNCaP. 

In vivo RK-33 in combination with radiation induces tumor regression in multiple mouse models of lung cancer. RK-33, at the dose used, is non-toxic in SCID mice. RK-33-treated mice do not exhibit any discernable morphological changes[1].


Cell Research:


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  • Cell lines: MDA-MB-231 cells
  • Concentrations: 7.5 μM
  • Incubation Time: 12 h
  • Method:

    Healthy, 60-70% confluent MDA-MB-231 cells are transduced with shDDX3 lentivirus particles. Knockdown of DDX3 expression is confirmed both by qRT-PCR and immunoblotting. MDA-MB-231 cells are treated with RK-33 (7.5 μM) for 12 h and harvested for RNA. Microarray experiments are performed.

    (Only for Reference)
Animal Research:


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  • Animal Models: SCID mice
  • Dosages: 20 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 85 mg/mL (198.39 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 428.44


CAS No. 1070773-09-9
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC=C(C=C1)CN2C=NC3=C2N=CN=C4C3=NC(=O)N4CC5=CC=C(C=C5)OC

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID