Favipiravir (T-705)

Name: Favipiravir (T-705)
Brand: Selleck Chemicals
SKU: S7975
Rating: 5 (3 reviews)

For research use only. Not for use in humans.

Catalog No.S7975

3 publications

Molecular Weight(MW): 157.1

Favipiravir (T-705) is a potent and selective RNA-dependent RNA polymerase inhibitor, used to treat influenza virus infections.

Selleck's Favipiravir (T-705) has been cited by 3 publications

Antiviral Res, 2019, 170:104570

PubMed: 31362004 ( click the link to review the publication )

Cell Physiol Biochem, 2018, 49(1):381-394

PubMed: 30138929 ( click the link to review the publication )

Antiviral Res, 2017, 143:237-245

PubMed: 28465146 ( click the link to review the publication )

2 Customer Reviews

(A) BoDV-1 infection was measured by IFA. BoDV-1 P40 was detected with a primary monoclonal antibody (red), nuclei were stained with DAPI (blue), merged image (scale bars: 50 μm). Favipiravir: T-705

Cell Physiol Biochem, 2018, 49(1):381-394. Favipiravir (T-705) purchased from Selleck.

(B) IFA of Vero-rBoDV-1-Gluc cells treated with T-705. Vero-rBoDV-1-Gluc cells were treated as indicated and the cells were stained with an anti-P antibody (shown in green) and DAPI (shown in blue). Bars, 50 μm.

Antiviral Res, 2017, 143:237-245. Favipiravir (T-705) purchased from Selleck.

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description

Favipiravir (T-705) is a potent and selective RNA-dependent RNA polymerase inhibitor, used to treat influenza virus infections.

Targets

RNA-dependent RNA polymerase ^[1]

In vitro

Favipiravir shows anti-influenza virus activities with IC50 ranged from 0.013 to 0.48 μg/ml for the influenza A viruses, from 0.039 to 0.089 μg/ml for the influenza B viruses, and from 0.030 to 0.057 μg/ml for the influenza C viruses. In mammalian cell lines (MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells), Favipiravir shows no cytotoxicity at concentrations up to 1,000 μg/ml. ^[1] In MDCK cells inoculated with seasonal influenza A (H1N1) viruses, Favipiravir induces lethal mutagenesis. ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
MDCK cells	NUHuOHhbTnWwY4Tpc44h[XO\|YYm=		NHXnO\|hKdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIH;mJIlv\my3ZX76ZUBCKH[rcoXzJEhCN0ixbnegT49v\y9{MUOvNFMpUDWQMTmpJIFv\CCrbn\seYVvgmFiQTD2bZJ2eyBqQT;Bco4hSXKkb4KvOk83OCiKMl6yLUkhcHmkcnnkJJZqenW\|IHnuJG1FS0tiY3XscJMh[nlibnX1eJJidCC{ZXSgeZB1[WunIHHzd4F6	NY\pT2hJOTdzOUS4N\|I>
Vero cells	NUGxSmV6TnWwY4Tpc44h[XO\|YYm=	NWn0U\|ZsPy16IHThfZM>	NXq3UohQSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUnWwaX6geolzfXNiQ3Hu[IllNTFiaX6gWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW\|LXnu[JVk\WRidnnzeYFtKGO7dH;wZZRpcWNiZX\m[YN1KGGodHXyJFchfG9iODDkZZl{	NFfUdW8yPzZyNk[5NS=>
MDCK cells	NYXQenE5TnWwY4Tpc44h[XO\|YYm=		NHHKOVZKdmirYnn0bY9vKG:oIHnu[ox2\W68YTDBJJZqenW\|IDjBM4R2[2txTXnucoV{d3SjL{G1NlUwOTl6MTCoTFVPOSlrIILldIxq[2G2aX;uJIlvKE2GQ1ugZ4VtdHNiYomgcoV2fHKjbDDy[YQhfXC2YXvlJIF{e2G7	M3XmfVE4OTl2OEOy

... Click to View More Cell Line Experimental Data

In vivo

In influenza virus-infected mice, Favipiravir (200 mg/kg/day, p.o.) protects the mice from death from influenza virus infection. ^[1] In mice experimentally infected with Ebola virus, Favipiravir efficiently blocks viral production, reaching an antiviral effectiveness of 95% and 99.6% at 2 and 6days after initiation of treatment, respectively. ^[3]

Protocol

Cell Research:^[1]

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Cell lines: MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells
Concentrations: 1000 μg/mL
Incubation Time: 3 days
Method: The cytotoxicity of T-705 is evaluated by an assay with XTT. XTT is converted to aqueous formazan by an enzyme in MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells. The compounds are diluted to the appropriate concentrations (volume, 100 μl) with test medium (EMEM containing 10% FCS) in 96-well culture plates in which each well contains a concentration of 2 × 10³ cells/100 μL. The test plates are incubated for 3 days at 37°C in 100% humidity and 5% CO₂. After 3 days, 50 μl of the XTT reagent (1 mg/ml in FCS-free EMEM containing 5 mM phenazine methosulfate) is added, and the reaction product is assayed by measurement of the absorbance at 450 nm with a microplate reader. Cytotoxicity is expressed as the 50% cell-inhibitory concentration (CC50).
(Only for Reference)

Animal Research:^[1]

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Animal Models: Mice infected with influenza virus A/PR/8/34
Formulation: 0.5% methylcellulose
Dosages: 200 mg/kg/day
Administration: p.o.
(Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	31 mg/mL (197.32 mM)
	Ethanol	22 mg/mL warmed (140.03 mM)
	Water	5 mg/mL warmed (31.82 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Favipiravir (T-705) SDF

Molecular Weight	157.1
Formula	C₅H₄FN₃O₂
CAS No.	259793-96-9
Storage	3 years -20°C powder
Storage	2 years -80°C in solvent
Synonyms	N/A
Smiles	NC(=O)C1=NC(=CN=C1O)F

Bio Calculators

Molarity Calculator

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Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
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C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2019-10-14)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03394209	Completed	Drug: Favipiravir\|Drug: Oseltamivir 75Mg Capsule	Influenza Human\|Critical Illness\|Influenza	Capital Medical University\|Beijing Institute of Pharmacology and Toxicology\|University of Oxford\|Centers for Disease Control and Prevention China	February 6 2018	Phase 2
NCT02739477	Terminated	Drug: Favipiravir	Ebola Virus Survivor	Institut National de la Santé Et de la Recherche Médicale France	April 2016	Phase 2
NCT02329054	Completed	Drug: Favipiravir	Ebola Virus Disease	Institut National de la Santé Et de la Recherche Médicale France	December 2014	Phase 2
NCT02026349	Completed	Drug: favipiravir\|Drug: Placebo	Influenza	MDVI LLC\|MediVector Inc.	January 2014	Phase 3
NCT02008344	Completed	Drug: favipiravir\|Drug: placebo	Influenza	MDVI LLC\|MediVector Inc.	December 2013	Phase 3
NCT01728753	Completed	Drug: Placebo\|Drug: Favipiravir	Influenza	MDVI LLC\|United States Department of Defense	November 2012	Phase 1\|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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DNA/RNA Synthesis Signaling Pathway Map

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Favipiravir (T-705)