Favipiravir (T-705)

For research use only.

Catalog No.S7975

9 publications

CAS No. 259793-96-9

Favipiravir (T-705) is a potent and selective RNA-dependent RNA polymerase inhibitor, used to treat influenza virus infections.

Selleck's Favipiravir (T-705) has been cited by 9 publications

Viruses, 2020, 22;12(3) pii: E351

PubMed: 32235763 ( click the link to review the publication )

Antiviral Res, 2020, 182:104902

PubMed: 32739404 ( click the link to review the publication )

Mol Ther Methods Clin Dev, 2019, 14:47-55

PubMed: 31309127 ( click the link to review the publication )

PLoS Negl Trop Dis, 2019, 13(11):e0007904

PubMed: 31751340 ( click the link to review the publication )

Antiviral Res, 2019, 165:55-64

PubMed: 30885750 ( click the link to review the publication )

Antiviral Res, 2019, 170:104570

PubMed: 31362004 ( click the link to review the publication )

Cell Physiol Biochem, 2018, 49(1):381-394

PubMed: 30138929 ( click the link to review the publication )

Antiviral Res, 2017, 143:237-245

PubMed: 28465146 ( click the link to review the publication )

Antivir Chem Chemother, 2017, 25(3):83-89

PubMed: 29096526 ( click the link to review the publication )

2 Customer Reviews

(A) BoDV-1 infection was measured by IFA. BoDV-1 P40 was detected with a primary monoclonal antibody (red), nuclei were stained with DAPI (blue), merged image (scale bars: 50 μm). Favipiravir: T-705

Cell Physiol Biochem, 2018, 49(1):381-394. Favipiravir (T-705) purchased from Selleck.

(B) IFA of Vero-rBoDV-1-Gluc cells treated with T-705. Vero-rBoDV-1-Gluc cells were treated as indicated and the cells were stained with an anti-P antibody (shown in green) and DAPI (shown in blue). Bars, 50 μm.

Antiviral Res, 2017, 143:237-245. Favipiravir (T-705) purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description

Favipiravir (T-705) is a potent and selective RNA-dependent RNA polymerase inhibitor, used to treat influenza virus infections.

Targets

RNA-dependent RNA polymerase ^[1]

In vitro

Favipiravir shows anti-influenza virus activities with IC50 ranged from 0.013 to 0.48 μg/ml for the influenza A viruses, from 0.039 to 0.089 μg/ml for the influenza B viruses, and from 0.030 to 0.057 μg/ml for the influenza C viruses. In mammalian cell lines (MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells), Favipiravir shows no cytotoxicity at concentrations up to 1,000 μg/ml. ^[1] In MDCK cells inoculated with seasonal influenza A (H1N1) viruses, Favipiravir induces lethal mutagenesis. ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
MDCK cells	MUnGeY5kfGmxbjDhd5NigQ>?		NIi1bG1KdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIH;mJIlv\my3ZX76ZUBCKH[rcoXzJEhCN0ixbnegT49v\y9{MUOvNFMpUDWQMTmpJIFv\CCrbn\seYVvgmFiQTD2bZJ2eyBqQT;Bco4hSXKkb4KvOk83OCiKMl6yLUkhcHmkcnnkJJZqenW\|IHnuJG1FS0tiY3XscJMh[nlibnX1eJJidCC{ZXSgeZB1[WunIHHzd4F6	NIXQd2YyPzF7NEizNi=>
Vero cells	NGTaV3ZHfW6ldHnvckBie3OjeR?=	NFPGTXQ4NThiZHH5dy=>	NHLmNYRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBLfW6rbjD2bZJ2eyCFYX7kbYQuOSCrbjDW[ZJwKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CC4aYP1ZYwh[3m2b4DheIhq[yCnZn\lZ5Qh[W[2ZYKgO{B1dyB6IHThfZM>	M1ewfVE4PjB4Nkmx
MDCK cells	Mlj1SpVv[3Srb36gZZN{[Xl?		NX3DUmU5UW6qaXLpeIlwdiCxZjDpcoZtfWWwenGgRUB3cXK3czCoRU9lfWOtL13pco5me2:2YT:xOVI2NzF7OEGgLGg2VjFrKTDy[ZBtcWOjdHnvckBqdiCPRFPLJINmdGy\|IHL5JI5mfXS{YXygdoVlKHWydHHr[UBie3OjeR?=	MYKxO\|E6PDh\|Mh?=

... Click to View More Cell Line Experimental Data

In vivo

In influenza virus-infected mice, Favipiravir (200 mg/kg/day, p.o.) protects the mice from death from influenza virus infection. ^[1] In mice experimentally infected with Ebola virus, Favipiravir efficiently blocks viral production, reaching an antiviral effectiveness of 95% and 99.6% at 2 and 6days after initiation of treatment, respectively. ^[3]

Protocol

Cell Research:^[1]	- Collapse Cell lines: MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells Concentrations: 1000 μg/mL Incubation Time: 3 days Method: The cytotoxicity of T-705 is evaluated by an assay with XTT. XTT is converted to aqueous formazan by an enzyme in MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells. The compounds are diluted to the appropriate concentrations (volume, 100 μl) with test medium (EMEM containing 10% FCS) in 96-well culture plates in which each well contains a concentration of 2 × 10³ cells/100 μL. The test plates are incubated for 3 days at 37°C in 100% humidity and 5% CO₂. After 3 days, 50 μl of the XTT reagent (1 mg/ml in FCS-free EMEM containing 5 mM phenazine methosulfate) is added, and the reaction product is assayed by measurement of the absorbance at 450 nm with a microplate reader. Cytotoxicity is expressed as the 50% cell-inhibitory concentration (CC50). (Only for Reference)
Animal Research:^[1]	- Collapse Animal Models: Mice infected with influenza virus A/PR/8/34 Dosages: 200 mg/kg/day Administration: p.o. (Only for Reference)

Cell Research:^[1]

- Collapse

Cell lines: MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells
Concentrations: 1000 μg/mL
Incubation Time: 3 days
Method: The cytotoxicity of T-705 is evaluated by an assay with XTT. XTT is converted to aqueous formazan by an enzyme in MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells. The compounds are diluted to the appropriate concentrations (volume, 100 μl) with test medium (EMEM containing 10% FCS) in 96-well culture plates in which each well contains a concentration of 2 × 10³ cells/100 μL. The test plates are incubated for 3 days at 37°C in 100% humidity and 5% CO₂. After 3 days, 50 μl of the XTT reagent (1 mg/ml in FCS-free EMEM containing 5 mM phenazine methosulfate) is added, and the reaction product is assayed by measurement of the absorbance at 450 nm with a microplate reader. Cytotoxicity is expressed as the 50% cell-inhibitory concentration (CC50).
(Only for Reference)

Animal Research:^[1]

- Collapse

Animal Models: Mice infected with influenza virus A/PR/8/34
Dosages: 200 mg/kg/day
Administration: p.o.
(Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	31 mg/mL (197.32 mM)
	Water	5 mg/mL warmed (31.82 mM)
	Ethanol	'22 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Favipiravir (T-705) SDF

Molecular Weight	157.1
Formula	C₅H₄FN₃O₂
CAS No.	259793-96-9
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	C1=C(N=C(C(=O)N1)C(=O)N)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-09-01)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04444986	Completed	Drug: FAVIR 200 MG FT\|Drug: AVIGAN 200 mg FT	Bioequivalence	Kocak Farma\|Novagenix Bioanalytical Drug R&D Center\|Farmagen Ar-Ge Biyot. Ltd. Sti	June 5 2020	Phase 1
NCT04358549	Recruiting	Drug: Favipiravir + Standard of Care\|Drug: Standard of Care	COVID-19	Fujifilm Pharmaceuticals U.S.A. Inc.	April 17 2020	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

DNA/RNA Synthesis Signaling Pathway Map

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Favipiravir (T-705)