Mizoribine
For research use only.
Catalog No.S1384 Synonyms: Bredinin, NSC 289637
2 publications
CAS No. 50924-49-7
Mizoribine (Bredinin, NSC 289637) is an imidazole nucleoside and an immunosuppressant that inhibits HCV RNA replication with IC50 of ~ 100 μM for anti-HCV activity. Mizoribine is a selective inhibitor of inosine-5'-monophosphate dehydrogenase (IMPDH) and guanosine monophosphate synthetase. Mizoribine also inhibits SARS-CoV.
Selleck's Mizoribine has been cited by 2 publications
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Biological Activity
| Description | Mizoribine (Bredinin, NSC 289637) is an imidazole nucleoside and an immunosuppressant that inhibits HCV RNA replication with IC50 of ~ 100 μM for anti-HCV activity. Mizoribine is a selective inhibitor of inosine-5'-monophosphate dehydrogenase (IMPDH) and guanosine monophosphate synthetase. Mizoribine also inhibits SARS-CoV. | |
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| Targets |
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| In vitro |
Mizoribine (1-50 mg/mL) is able to inhibit T cell proliferation by 10-100% in a dose-dependent fashion to all stimuli tested. Mizoribine leads to a decrease in intracellular GTP levels, and that repletion of GTP reversed its antiproliferative effects. [1] Mizoribine causes a dose-dependent inhibition of T cell proliferation, which is reversible with the addition of 50 mM guanosine to replete guanine ribonucleotide pools. Mizoribine selectively inhibits guanine ribonucleotide formation in purified T cells, whereas the effect of 6-mercaptopurine (6MP) appears to be more dependent on adenine ribonucleotide depletion. [2] Mizoribine inhibits HCV RNA replication with IC50 of 100 μM. [3] |
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| In vivo | Mizoribine (5 or 10 mg/kg) suppresses urinary albumin excretion in the rats. Mizoribine (5 or 10 mg/kg) inhibits the development of glomerulosclerosis, interstitial fibrosis and macrophage infiltration in the kidney in the rats. Mizoribine (5 or 10 mg/kg) increases the expression of MCP-1, OPN and TGF-β1 mRNA in untreated OLETF rats. [4] |
Protocol
Solubility (25°C)
| In vitro | Water | 51 mg/mL (196.74 mM) |
|---|---|---|
| DMSO | 1 mg/mL (3.85 mM) | |
| Ethanol | Insoluble |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 259.22 |
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| Formula | C9H13N3O6 |
| CAS No. | 50924-49-7 |
| Storage |
powder in solvent |
| Synonyms | Bredinin, NSC 289637 |
| Smiles | C1=NC(=C(N1C2C(C(C(O2)CO)O)O)O)C(=O)N |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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