Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor - Fungal inhibitor Tubercidin For research use only.

Tubercidin

Synonyms: 7-deaza-adenosine

Tubercidin (7-deaza-adenosine), an adenosine analogue, is a nucleoside antibiotic. It is incorporated into DNA and inhibits polymerases, thereby inhibiting DNA replication and RNA and protein synthesis. This agent also exhibits antifungal and antiviral activities.

Tubercidin Chemical Structure

Tubercidin Chemical Structure

CAS: 69-33-0

Selleck's Tubercidin has been cited by 1 publication

Purity & Quality Control

Batch: S809501 DMSO] 53 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.52%
99.52

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Signaling Pathway

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 Function assay Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro, IC50=0.04μM 1310744
H.Ep.2 Function assay Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of H.Ep.2 cells in vitro, IC50=0.06μM 1310744
HFF cells Cytotoxicity assay Cytotoxicity against uninfected human foreskin fibroblast(HFF cells), IC50=0.4μM 2846837
KB cells Cytotoxicity assay Cytotoxicity against human neoplastic cell line(KB cells), IC50=0.6μM 2846837
L1210 Cytotoxicity assay In vitro cytotoxicity was evaluated against the L1210 Murine leukemic cells, IC50=0.04μM 2913300
HFF cells Cytotoxicity assay Cytotoxicity was evaluated against the Human diploid cells (HFF), IC50=0.3μM 2913300
H.Ep.2 Function assay Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control. , IC50=0.002μM 6708054
L1210 Function assay Tested for in vitro cell growth inhibition of L1210 cells, IC50=0.043μM 8254613
H.Ep.2 Function assay Tested for in vitro cell growth inhibition of H. Ep. 2 cells, IC50=0.06μM 8254613
L2 Growth inhibition assay 37 uM Growth inhibition of mouse L2 cells at 37 uM 17977728
HeLaS3 Antiviral assay 2 days Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days, IC50=0.03μM 20964406
HeLaS3 Cytotoxicity assay 48 hrs Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay, IC50=0.4μM 20964406
HeLaS3 Cytotoxicity assay 24 hrs Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay, IC50=0.819μM 20964406
HeLaS3 Cytotoxicity assay 7 hrs Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay, IC50=1.44μM 20964406
A549 Cytostatic assay 5 days Cytostatic activity against human A549 cells after 5 days by SRB assay, GI50=0.001μM 21711054
HCT116 Cytostatic assay 5 days Cytostatic activity against human HCT116 cells after 5 days by SRB assay, GI50=0.001μM 21711054
NCI-H23 Cytostatic assay 5 days Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay, GI50=0.011μM 21711054
HCT15 Cytostatic assay 5 days Cytostatic activity against human HCT15 cells after 5 days by SRB assay, GI50=0.011μM 21711054
DU145 Cytostatic assay 5 days Cytostatic activity against human DU145 cells after 5 days by SRB assay, GI50=0.018μM 21711054
MT4 Cytostatic assay 5 days Cytostatic activity against human MT4 cells after 5 days by SRB assay, GI50=0.021μM 21711054
PC3 Cytostatic assay 5 days Cytostatic activity against human PC3 cells after 5 days by SRB assay, GI50=0.048μM 21711054
Hs578 Cytostatic assay 5 days Cytostatic activity against human Hs578 cells after 5 days by SRB assay, GI50=0.098μM 21711054
HEK293 Function assay Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay, IC50=0.88μM 21870828
MRC5 Antitrypanosomal assay 7 days Antitrypanosomal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 harboring beta-galactosidase infected in human MRC5 cells after 7 days by resazurin dye-based assay, EC50=0.34μM 30677668
MRC5 Cytotoxicity assay 3 days Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay, EC50=2.23μM 30677668
Vero Function assay Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay, EC50=1.3μM 31549836
Vero Function assay Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay, EC50=1.3μM 31549836
Vero Function assay 5 days Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay, EC50=1.3μM 31549836
Vero Function assay Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield, EC50=1.3μM 31549836
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Biological Activity

Description Tubercidin (7-deaza-adenosine), an adenosine analogue, is a nucleoside antibiotic. It is incorporated into DNA and inhibits polymerases, thereby inhibiting DNA replication and RNA and protein synthesis. This agent also exhibits antifungal and antiviral activities.
In vitro
In vitro Continuous exposure for 14 days to tubercidin alone is highly toxic to both human CFU-GM and BFU-E. The IC50s of tubercidin are 3.4±1.7 and 3.7±0.2 nM for CFU-GM and BFU-E, respectively. Tubercidin also has a direct dose-dependent inhibitory effect on myeloid and erythroid human bone marrow progenitor cells in vitro[1].
Cell Research Cell lines Human bone marrow cells
Concentrations 10 nM
Incubation Time 14 days
Method

Human bone marrow cells were obtained from healthy volunteers. In the protection studies, cells are exposed to 10 nM of tubercidin in the presence or absence of various doses of NBMPR-P. Cells are continuously exposed to drugs for 14 days in a humidified atmosphere of 5% C02-95% air at 37°C. Colonies of CFU-GM (250 cells) and BFU-E (>30 hemoglobinized cells) are counted with an inverted microscope.
In Vivo
In vivo Tubercidin at the dose regimen (5 mg/kg everyday for 4 days) is lethal when used alone. Some of the studies show that the mortality from tubercidin is primarily due to hepatic and renal injuries and, to a lesser extent, damage to the pancreas. Coadministration of NBMPR-P at 25 mg/kg per day completely protects the mice (100% survival) from this lethal dose regimen of tubercidin[1].
Animal Research Animal Models CD1 mice
Dosages 5 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 266.25 Formula

C11H14N4O4
CAS No. 69-33-0 SDF Download Tubercidin SDF
Smiles C1=CN(C2=NC=NC(=C21)N)C3C(C(C(O3)CO)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 53 mg/mL ( (199.06 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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