Name: Tubercidin
Brand: Selleck Chemicals
SKU: S8095
Availability: InStock
Rating: 5 (1 reviews)

Jump to

Quality Control

Batch: S809501 DMSO]53 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.52%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.52

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN Topoisomerase PPAR Sirtuin Casein Kinase eIF
Other DNA/RNA Synthesis Inhibitors	CX-5461 (Pidnarulex) B02 SCR7 Favipiravir (T-705) EED226 BMH-21 RK-33 Triapine (3-AP) Carmofur YK-4-279

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
L1210	Function assay			Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro, IC50=0.04μM	1310744
H.Ep.2	Function assay			Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of H.Ep.2 cells in vitro, IC50=0.06μM	1310744
HFF cells	Cytotoxicity assay			Cytotoxicity against uninfected human foreskin fibroblast(HFF cells), IC50=0.4μM	2846837
KB cells	Cytotoxicity assay			Cytotoxicity against human neoplastic cell line(KB cells), IC50=0.6μM	2846837
L1210	Cytotoxicity assay			In vitro cytotoxicity was evaluated against the L1210 Murine leukemic cells, IC50=0.04μM	2913300
HFF cells	Cytotoxicity assay			Cytotoxicity was evaluated against the Human diploid cells (HFF), IC50=0.3μM	2913300
H.Ep.2	Function assay			Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control. , IC50=0.002μM	6708054
L1210	Function assay			Tested for in vitro cell growth inhibition of L1210 cells, IC50=0.043μM	8254613
H.Ep.2	Function assay			Tested for in vitro cell growth inhibition of H. Ep. 2 cells, IC50=0.06μM	8254613
L2	Growth inhibition assay	37 uM		Growth inhibition of mouse L2 cells at 37 uM	17977728
HeLaS3	Antiviral assay		2 days	Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days, IC50=0.03μM	20964406
HeLaS3	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay, IC50=0.4μM	20964406
HeLaS3	Cytotoxicity assay		24 hrs	Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay, IC50=0.819μM	20964406
HeLaS3	Cytotoxicity assay		7 hrs	Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay, IC50=1.44μM	20964406
A549	Cytostatic assay		5 days	Cytostatic activity against human A549 cells after 5 days by SRB assay, GI50=0.001μM	21711054
HCT116	Cytostatic assay		5 days	Cytostatic activity against human HCT116 cells after 5 days by SRB assay, GI50=0.001μM	21711054
NCI-H23	Cytostatic assay		5 days	Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay, GI50=0.011μM	21711054
HCT15	Cytostatic assay		5 days	Cytostatic activity against human HCT15 cells after 5 days by SRB assay, GI50=0.011μM	21711054
DU145	Cytostatic assay		5 days	Cytostatic activity against human DU145 cells after 5 days by SRB assay, GI50=0.018μM	21711054
MT4	Cytostatic assay		5 days	Cytostatic activity against human MT4 cells after 5 days by SRB assay, GI50=0.021μM	21711054
PC3	Cytostatic assay		5 days	Cytostatic activity against human PC3 cells after 5 days by SRB assay, GI50=0.048μM	21711054
Hs578	Cytostatic assay		5 days	Cytostatic activity against human Hs578 cells after 5 days by SRB assay, GI50=0.098μM	21711054
HEK293	Function assay			Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay, IC50=0.88μM	21870828
MRC5	Antitrypanosomal assay		7 days	Antitrypanosomal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 harboring beta-galactosidase infected in human MRC5 cells after 7 days by resazurin dye-based assay, EC50=0.34μM	30677668
MRC5	Cytotoxicity assay		3 days	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay, EC50=2.23μM	30677668
Vero	Function assay			Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay, EC50=1.3μM	31549836
Vero	Function assay			Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay, EC50=1.3μM	31549836
Vero	Function assay		5 days	Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay, EC50=1.3μM	31549836
Vero	Function assay			Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield, EC50=1.3μM	31549836
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 53 mg/mL (199.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.65mg/ml (9.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 53 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.265mg/ml (1.00mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	266.25	Formula	C₁₁H₁₄N₄O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	69-33-0	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	7-deaza-adenosine			Smiles	C1=CN(C2=NC=NC(=C21)N)C3C(C(C(O3)CO)O)O

Mechanism of Action

In vitro	Continuous exposure for 14 days to tubercidin alone is highly toxic to both human CFU-GM and BFU-E. The IC50s of tubercidin are 3.4±1.7 and 3.7±0.2 nM for CFU-GM and BFU-E, respectively. Tubercidin also has a direct dose-dependent inhibitory effect on myeloid and erythroid human bone marrow progenitor cells in vitro.
In vivo	Tubercidin at the dose regimen (5 mg/kg everyday for 4 days) is lethal when used alone. Some of the studies show that the mortality from tubercidin is primarily due to hepatic and renal injuries and, to a lesser extent, damage to the pancreas. Coadministration of NBMPR-P at 25 mg/kg per day completely protects the mice (100% survival) from this lethal dose regimen of tubercidin.
References	[1] https://pubmed.ncbi.nlm.nih.gov/2764531/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Tubercidin DNA/RNA Synthesis inhibitor

Chemical Structure

Molecular Weight: 266.25