Purvalanol A

For research use only.

Catalog No.S7793

7 publications

Purvalanol A Chemical Structure

Molecular Weight(MW): 388.89

Purvalanol A is a potent, and cell-permeable CDK inhibitor with IC50 of 4 nM, 70 nM, 35 nM, and 850 nM for cdc2-cyclin B, cdk2-cyclin A, cdk2-cyclin E, and cdk4-cyclin D1, respectively.

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Selleck's Purvalanol A has been cited by 7 publications

2 Customer Reviews

  • After 12 h PAB treatment, cells were treated with PAB in the absence and presence of RO-3306 or purvalanol A for 12 h and 36 h. (A) Expressions of p-histone h3 were detected by western blot.

    Cancer Lett, 2016, 383(2):295-308. Purvalanol A purchased from Selleck.

    (A)HeLa cells were treated with Nocodazole for 8 hours and then fixed. Before the cells were stained with phospho-specific antibody against S119 of Ajuba, they were pre-incubated with PBS (no peptide control), or non-phosphorylated (control) peptide, or the phosphorylated peptide used for immunizing rabbits. CDK1 inhibitors Purvalanol A (10 μM) together with MG132 (25 μM) were added 2 h before the cells were fixed (bottom two rows).(B)Experiments were done similarly as in (A) with phospho-specific antibody against Ser175 of Ajuba.

    J Biol Chem, 2016, 291(28):14761-72.. Purvalanol A purchased from Selleck.

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Biological Activity

Description Purvalanol A is a potent, and cell-permeable CDK inhibitor with IC50 of 4 nM, 70 nM, 35 nM, and 850 nM for cdc2-cyclin B, cdk2-cyclin A, cdk2-cyclin E, and cdk4-cyclin D1, respectively.
Cdc2/CyclinB [1]
CDK2/CyclinE [1] CDK2/CyclinA [1] CDK4/CyclinD1 [1]
4 nM 35 nM 70 nM 850 nM
In vitro

Purvalanol A decreases cell viability in dose-dependent manner in MCF-7 and MDA-MB-231 cell lines. Purvalanol A induces cell viability loss by 50 % in MCF-7 cells but MDA-MB-231 cells sre less sensitive to Purvalanol A (32 % decreases in cell viability). Purvalanol A induces mitochondria-mediated apoptosis in MCF-7 and MDA-MB-231 cells.[2] Purvalanol A effectively prevents c-Src-mediated transformation by inhibiting both cell cycle progression and c-Src signaling, and effectively suppresses the anchorage independent growth of some human cancer cells in which c-Src is up-regulated. Purvalanol A has a stronger inhibitory effect on the anchorage-independent growth of HT29 and SW480 human colon cancer cells. [3]


Cell Research:[2]
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  • Cell lines: MCF-7 and MDA-MB 231 breast cancer cells
  • Concentrations: 100 μM
  • Incubation Time: 24 h
  • Method: Cells are seeded at 10000 density in 96-well plates and treated with various concentrations of Purvalanol A (0-100 μM) for 24 h. Cells are exposed to 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet- razolium bromide dye (5 mg/mL) and are incubated at 37℃ for 4 h. In order to solubilize the formazan crystals 100 μL DMSO is added. Absorbance is determined at 570 nm spectrophotometrically.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 60 mg/mL warmed (154.28 mM)
Ethanol 13 mg/mL warmed (33.42 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 388.89


CAS No. 212844-53-6
Storage powder
in solvent
Synonyms N/A
Smiles CC(C)C(CO)NC1=NC(=C2N=C[N](C(C)C)C2=N1)NC3=CC=CC(=C3)Cl

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID