Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

Size Price Stock Quantity  
USD 127 In stock
USD 397 In stock
USD 997 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

3 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NGjkU4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2VTmWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzNyOUWg{txO MYLTRW5ITVJ?
human H460a cells MkLDVJJwdGmoZYLheIlwdiCjc4PhfS=> MkDyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKNE[wZUBk\WyuczygTWM2OD1zLkC0JO69VQ>? NX;WNHVtOTd|MEO0NlE>
human MDA435 cells MlTVVJJwdGmoZYLheIlwdiCjc4PhfS=> NYPPVFc6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGE1OzViY3XscJMtKEmFNUC9NU4yPCEQvF2= NFfKenMyPzNyM{SyNS=>
human HCT116 cells NVPrNI5LWHKxbHnm[ZJifGmxbjDhd5NigQ>? MUXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczygTWM2OD1zLkG0JO69VQ>? MVexO|MxOzR{MR?=
human H4 cell NHHxVYRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NY[1TohtUW6qaXLpeIlwdiCxZjDoeY1idiCKNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNVU5PzVizszN NHT5T21USU6JRWK=
human PA-1 cell MnfkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX;Jcohq[mm2aX;uJI9nKGi3bXHuJHBCNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKyOlU1KM7:TR?= NFji[oZUSU6JRWK=
human T98G cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW\wfHlGUW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjhzMjFOwG0> M1;OPHNCVkeHUh?=
human SW1710 cell M2fCUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1jNUmlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43OTRyODFOwG0> NXLlOFc1W0GQR1XS
human U251 cell NYHrSldMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWnJcohq[mm2aX;uJI9nKGi3bXHuJHUzPTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLk[zO|cyKM7:TR?= MlvXV2FPT0WU
human ALL-PO cell Ml\rS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFvv[YlKdmirYnn0bY9vKG:oIHj1cYFvKEGOTD3QU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:OS57MEixN{DPxE1? Ml;JV2FPT0WU
human RKO cells NEjacnJRem:uaX\ldoF1cW:wIHHzd4F6 MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyxiSVO1NF0zKM7:TR?= MWmxO|MxOzR{MR?=
human SK-N-DZ cell M1zvVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWHj[XFlUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWRbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5yMUi3OkDPxE1? M1yzNXNCVkeHUh?=
human A4-Fuk cell NFT1fnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlryTY5pcWKrdHnvckBw\iCqdX3hckBCPC2IdXugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlA2PjB2IN88US=> M3noUnNCVkeHUh?=
human SW480 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 M{jZN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOsJGlEPTB;Mj6yJO69VQ>? NYHxbGtqOTd|MEO0NlE>
human 639-V cell NIf6Vm1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1LKN2lvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlI4PTN5IN88US=> MUnTRW5ITVJ?
human Hs-578-T cell M1jaVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVHJcohq[mm2aX;uJI9nKGi3bXHuJGh{NTV5OD3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4zPzd7OTFOwG0> NF;kU2FUSU6JRWK=
human UM-UC-3 cell MmH0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWPJcohq[mm2aX;uJI9nKGi3bXHuJHVONVWFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVIvPzN7M{[g{txO MXLTRW5ITVJ?
human BPH-1 cell NWrvdI5uT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlXiTY5pcWKrdHnvckBw\iCqdX3hckBDWEhvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuPVc1PTVizszN NH3oNppUSU6JRWK=
human 5637 cell MofCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF7UVFRKdmirYnn0bY9vKG:oIHj1cYFvKDV4M{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlA5PTN{IN88US=> NUm0OWtDW0GQR1XS
human 8-MG-BA cell M4rISGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mm\tTY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0{NjF2MES5JO69VQ>? NUK1eppmW0GQR1XS
human HCT116 cells MmDnR5l1d3SxeHnjxsBie3OjeR?= NWDDdJIxPSCmYYnz MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOUBl[Xm|IHL5JG1VWyCjc4Phfg+9lCCLQ{WwQVMvOiEQvF2= MUOyNlMzPjF4OB?=
human LB1047-RCC cell NI\tZZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVjJcohq[mm2aX;uJI9nKGi3bXHuJGxDOTB2Nz3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjJ|NUCxJO69VQ>? NEfLVY5USU6JRWK=
human KOSC-2 cell MonnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVy5e2JJUW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR2Nk[2NkDPxE1? MlLLV2FPT0WU
human COR-L88 cell MonSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVTJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OOEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlQ4PTh|IN88US=> MWHTRW5ITVJ?
human PSN1 cell NE\6SItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;GTY5pcWKrdHnvckBw\iCqdX3hckBRW05zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xNFgxPSEQvF2= MUHTRW5ITVJ?
human LXF-289 cell MlfmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXzJcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlIyPzZizszN M3PN[nNCVkeHUh?=
human MN-60 cell NUPzdnBjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2PQdmlvcGmkaYTpc44hd2ZiaIXtZY4hVU5vNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlM4PjJ|IN88US=> NFnXZnRUSU6JRWK=
human NEC8 cell MlX1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1;WO2lvcGmkaYTpc44hd2ZiaIXtZY4hVkWFODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFcxPzZizszN M3POcnNCVkeHUh?=
human SJSA1 cells M3Ptd3Bzd2yrZnXyZZRqd25iYYPzZZk> MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOMU1GxJINmdGy|LDDJR|UxRTRwNUWg{txO MU[xO|MxOzR{MR?=
human NCI-H2405 cell NFzxO25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4fX[2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyOFA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC55NES5N{DPxE1? MoTMV2FPT0WU
human YH-13 cell M3jicmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUfBfIJXUW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzV5N{[g{txO NYTZPVFyW0GQR1XS
human SK-MEL-2 cell NVjF[nBbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVXVOXBXUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwMEm2N|Ih|pyv MVLTRW5ITVJ?
human NB13 cell NXi3dHFuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGPNd|FKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> MYLTRW5ITVJ?
human GAMG cell NW\xSYZiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4n6TWlvcGmkaYTpc44hd2ZiaIXtZY4hT0GPRzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|cyPSEQvF2= MkW5V2FPT0WU
human NCI-H1792 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnuxTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG3PVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjR5OUW0JO69VQ>? M3LLSHNCVkeHUh?=
human KP-4 cell MnXBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYXJcohq[mm2aX;uJI9nKGi3bXHuJGtRNTRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lk[5PFI{KM7:TR?= M4LlfnNCVkeHUh?=
human HGC-27 cell M1;LOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHznRVJKdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzNyN{eg{txO M3XEXXNCVkeHUh?=
human GCT cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVLNXYF3UW6qaXLpeIlwdiCxZjDoeY1idiCJQ2SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlg2OTJzIN88US=> MVrTRW5ITVJ?
human PFSK-1 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXewZ2N2UW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njh4MU[4JO69VQ>? Mn3uV2FPT0WU
human A172 cell M1PRe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYjJcohq[mm2aX;uJI9nKGi3bXHuJGEyPzJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkmwO|kh|ryP NV6yZohqW0GQR1XS
human HO-1-N-1 cell NGrxT|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MornTY5pcWKrdHnvckBw\iCqdX3hckBJVy1zLV6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTV2MkWg{txO NUTZNmx7W0GQR1XS
human HN cell M4jCXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3nvXWlvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lkm3NFk4KM7:TR?= M1q0XXNCVkeHUh?=
human IST-MES1 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NY\nVmdjUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StUWVUOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUe1NlEh|ryP Mmn6V2FPT0WU
human TGW cell MlexS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWjxbZhSUW6qaXLpeIlwdiCxZjDoeY1idiCWR2egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2Pjh{IN88US=> M{jRZnNCVkeHUh?=
human COR-L23 cell NVL0W5pIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlM4OzVizszN M4PhenNCVkeHUh?=
human D-566MG cell MknQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1PBe2lvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvPzh6N{Og{txO NHHjXFFUSU6JRWK=
human SKG-IIIa cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkHpTY5pcWKrdHnvckBw\iCqdX3hckBUU0dvSVnJZUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvQDV3N{Kg{txO Mlv6V2FPT0WU
human NCI-H446 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1T5dGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OFYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjBzOEGg{txO NGHaUI5USU6JRWK=
human KYSE-270 cell NFvHclZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NITBSIJKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtNlcxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yM{i3NkDPxE1? M3[wfHNCVkeHUh?=
human CAL-39 cell NH7FVW1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4q5Z2lvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4{PTJ2IN88US=> M3HVeXNCVkeHUh?=
human IST-SL1 cell NFPpbGlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVzJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ{OzR7IN88US=> MmGwV2FPT0WU
human SF539 cell NUXGPHV2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUPJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz61OVE6PyEQvF2= M3nlU3NCVkeHUh?=
human TE-1 cell NYK1cZRDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2ex[WlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPFkxQTFizszN M1fhV3NCVkeHUh?=
human NB10 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3jmcmlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVg4PThizszN MYfTRW5ITVJ?
human 8505C cell MkHRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGDkdWVKdmirYnn0bY9vKG:oIHj1cYFvKDh3MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QThzNzFOwG0> MlHmV2FPT0WU
human DB cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGRDKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5yMUGzNUDPxE1? M13TdHNCVkeHUh?=
human NCI-H28 cell NEG3coVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVLlc5VyUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC53OUi4OEDPxE1? MVjTRW5ITVJ?
human LOXIMVI cell NFnXO|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVTJcohq[mm2aX;uJI9nKGi3bXHuJGxQYEmPVlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY{PzBzIN88US=> Mnq5V2FPT0WU
human ME-180 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXTJcohq[mm2aX;uJI9nKGi3bXHuJG1GNTF6MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuPFA{QTVizszN M3TnRnNCVkeHUh?=
human ES3 cell M1vQSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXXJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTlwMUK3PVQh|ryP NWHQT3V5W0GQR1XS
human SK-N-FI cell MnH4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHu0OZZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjRzNkm5JO69VQ>? NHfacFFUSU6JRWK=
human NBsusSR cell NGTWfY1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUTNXmU4UW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwMkOxPEDPxE1? MmLuV2FPT0WU
human MCF7 cell NYnvd3RGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6xPFQ6KM7:TR?= MlnSV2FPT0WU
human A549 cell M4fUOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkPxTY5pcWKrdHnvckBw\iCqdX3hckBCPTR7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlA3KM7:TR?= NHvuSFZUSU6JRWK=
human HuP-T4 cell NYfm[3BFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYrJcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlQzPzFizszN MnLsV2FPT0WU
human HSC-2 cell NHf5OYNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXTJcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGsO|g1OiEQvF2= MWDTRW5ITVJ?
human A253 cell MoDQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmH4TY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNlg{PCEQvF2= M3r3S3NCVkeHUh?=
human HCT-116 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4S3b2lvcGmkaYTpc44hd2ZiaIXtZY4hWENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlAxPDNizszN NUjyUGh6W0GQR1XS
human HCT-116 cell MnflS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NILoTmJKdmirYnn0bY9vKG:oIHj1cYFvKEiFVD2xNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQS54Nkig{txO MWLTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
+ Expand

CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
+ Expand
  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

CDK Signaling Pathway Map

Related CDK Products

Tags: buy Ro-3306 | Ro-3306 supplier | purchase Ro-3306 | Ro-3306 cost | Ro-3306 manufacturer | order Ro-3306 | Ro-3306 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID