Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NEHuXWhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M33Zb2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55M{C5OUDPxE1? MlTPV2FPT0WU
human H460a cells MVrQdo9tcW[ncnH0bY9vKGG|c3H5 M2ntUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSES2NIEh[2WubIOsJGlEPTB;MT6wOEDPxE1? M3v0elE4OzB|NEKx
human MDA435 cells MnTsVJJwdGmoZYLheIlwdiCjc4PhfS=> NX;F[GtPSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGE1OzViY3XscJMtKEmFNUC9NU4yPCEQvF2= NX3sO|E3OTd|MEO0NlE>
human HCT116 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 MknKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOsJGlEPTB;MT6xOEDPxE1? MnriNVc{ODN2MkG=
human H4 cell NHXIendIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXnGXpBPUW6qaXLpeIlwdiCxZjDoeY1idiCKNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNVU5PzVizszN M2TJVHNCVkeHUh?=
human PA-1 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF\kR2RKdmirYnn0bY9vKG:oIHj1cYFvKFCDLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIzPjV2IN88US=> NXnQbo9JW0GQR1XS
human T98G cell NHOzSotIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWrvNmRqUW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjhzMjFOwG0> NFL4[WdUSU6JRWK=
human SW1710 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYTJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlE1ODhizszN MWPTRW5ITVJ?
human U251 cell MkjRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVnSOndpUW6qaXLpeIlwdiCxZjDoeY1idiCXMkWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43Ozd5MTFOwG0> MV\TRW5ITVJ?
human ALL-PO cell NE\PPIlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H589yNKEmFNUC9NU46ODhzMzFOwG0> NUT5[GhXW0GQR1XS
human RKO cells MWXQdo9tcW[ncnH0bY9vKGG|c3H5 MknwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCUS1:gZ4VtdHNuIFnDOVA:OiEQvF2= MlTaNVc{ODN2MkG=
human SK-N-DZ cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4rXfGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODF6N{[g{txO MVfTRW5ITVJ?
human A4-Fuk cell NWrQZm1CT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnnITY5pcWKrdHnvckBw\iCqdX3hckBCPC2IdXugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlA2PjB2IN88US=> NEfLcG5USU6JRWK=
human SW480 cells NULJ[XdNWHKxbHnm[ZJifGmxbjDhd5NigQ>? MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZNEiwJINmdGy|LDDJR|UxRTJwMjFOwG0> Ml:wNVc{ODN2MkG=
human 639-V cell NWnTW4x{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrCbmtzUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOjd3M{eg{txO MUPTRW5ITVJ?
human Hs-578-T cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MojJTY5pcWKrdHnvckBw\iCqdX3hckBJey13N{itWEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOjd5OUmg{txO NUHrXHc{W0GQR1XS
human UM-UC-3 cell MkH1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXTYRoRFUW6qaXLpeIlwdiCxZjDoeY1idiCXTT3VR{0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1{LkezPVM3KM7:TR?= NUPDS5VoW0GQR1XS
human BPH-1 cell NWq3SlZpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYjJcohq[mm2aX;uJI9nKGi3bXHuJGJRUC1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj65O|Q2PSEQvF2= NGDSVI1USU6JRWK=
human 5637 cell NUjSeJA{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH\YeXRKdmirYnn0bY9vKG:oIHj1cYFvKDV4M{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlA5PTN{IN88US=> NVnqbHFCW0GQR1XS
human 8-MG-BA cell NVjy[Yp5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkDWTY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0{NjF2MES5JO69VQ>? MUPTRW5ITVJ?
human HCT116 cells MmnBR5l1d3SxeHnjxsBie3OjeR?= MWS1JIRigXN? NEPDO4pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5Nige,:jDDJR|UxRTNwMjFOwG0> MmLlNlI{OjZzNki=
human LB1047-RCC cell Mn3yS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXn4SWc6UW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yN|UxOSEQvF2= M3LYdHNCVkeHUh?=
human KOSC-2 cell NIjYdoZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MknZTY5pcWKrdHnvckBw\iCqdX3hckBMV1OFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlQ1PjZ4MjFOwG0> MWnTRW5ITVJ?
human COR-L88 cell M2\xRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkSyTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEi4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41PzV6MzFOwG0> MmTxV2FPT0WU
human PSN1 cell M4e0UWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFPL[3BKdmirYnn0bY9vKG:oIHj1cYFvKFCVTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlExQDB3IN88US=> NGO3N41USU6JRWK=
human LXF-289 cell Mk\VS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXHsRnNmUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5{MUe2JO69VQ>? MXXTRW5ITVJ?
human MN-60 cell MnWwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NULwN5ByUW6qaXLpeIlwdiCxZjDoeY1idiCPTj22NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvOzd4MkOg{txO M4HDOXNCVkeHUh?=
human NEC8 cell NET1eFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYrJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12LkS3NFc3KM7:TR?= M{P5WXNCVkeHUh?=
human SJSA1 cells M1;DTnBzd2yrZnXyZZRqd25iYYPzZZk> MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOMU1GxJINmdGy|LDDJR|UxRTRwNUWg{txO M{\SZVE4OzB|NEKx
human NCI-H2405 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17qSmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyOFA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC55NES5N{DPxE1? M2fRZnNCVkeHUh?=
human YH-13 cell NE\TXmpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkPWTY5pcWKrdHnvckBw\iCqdX3hckB[UC1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuO|U4PzZizszN M1nQTHNCVkeHUh?=
human SK-MEL-2 cell NG\UW3JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlLOTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvODl4M{Kg{rxu NFzkbWFUSU6JRWK=
human NB13 cell MnP3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkjmTY5pcWKrdHnvckBw\iCqdX3hckBPSjF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zNlc5PiEQvF2= MVvTRW5ITVJ?
human GAMG cell MnPhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEDmUFNKdmirYnn0bY9vKG:oIHj1cYFvKEeDTVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM4OTVizszN M3Hp[3NCVkeHUh?=
human NCI-H1792 cell M1;SPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFXZ[pdKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc6OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwNEe5OVQh|ryP MYDTRW5ITVJ?
human KP-4 cell Mo\yS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIDXflFKdmirYnn0bY9vKG:oIHj1cYFvKEuSLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlY6QDJ|IN88US=> NFz0V4JUSU6JRWK=
human HGC-27 cell M4XId2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{HJZmlvcGmkaYTpc44hd2ZiaIXtZY4hUEeFLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44OzB5NzFOwG0> M{njRnNCVkeHUh?=
human GCT cell NWm4b|hCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEPHV4dKdmirYnn0bY9vKG:oIHj1cYFvKEeFVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFUyOjFizszN NEHaUWFUSU6JRWK=
human PFSK-1 cell Mom0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NG\EeGpKdmirYnn0bY9vKG:oIHj1cYFvKFCIU1utNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQDZzNkig{txO Mom3V2FPT0WU
human A172 cell NGPGVoRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{W2OGlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVA4QSEQvF2= MUXTRW5ITVJ?
human HO-1-N-1 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUTP[HVYUW6qaXLpeIlwdiCxZjDoeY1idiCKTz2xMW4uOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUW0NlUh|ryP M2ftNnNCVkeHUh?=
human HN cell NHjBdm9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJGhPKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{C5O{DPxE1? Mn;xV2FPT0WU
human IST-MES1 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVnYTlVPUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StUWVUOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUe1NlEh|ryP NWLQTYFPW0GQR1XS
human TGW cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKGi3bXHuJHRIXyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwMEW2PFIh|ryP NVHMNItLW0GQR1XS
human COR-L23 cell M{PXNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mo\jTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEKzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{PzN3IN88US=> NWfNflF{W0GQR1XS
human D-566MG cell NFLHTW1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWP4XHM5UW6qaXLpeIlwdiCxZjDoeY1idiCGLUW2Om1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi55OEi3N{DPxE1? MnHPV2FPT0WU
human SKG-IIIa cell NFTQRXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJHNMTy2LSVnhJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok45PTV5MjFOwG0> MVPTRW5ITVJ?
human NCI-H446 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnLDTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSES0OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODF6MTFOwG0> NFvwRYlUSU6JRWK=
human KYSE-270 cell NYqyfmlkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYXWNoxDUW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEO4O|Ih|ryP MkLlV2FPT0WU
human CAL-39 cell NVS1V|hET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUHjPI9{UW6qaXLpeIlwdiCxZjDoeY1idiCFQVytN|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjN3MkSg{txO MWXTRW5ITVJ?
human IST-SL1 cell NGDaUoVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1THfmlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLWPMNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPDN|NEmg{txO Mly4V2FPT0WU
human SF539 cell Mo\FS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE\Yc45KdmirYnn0bY9vKG:oIHj1cYFvKFOINUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{42PTF7NzFOwG0> NG\UXlJUSU6JRWK=
human TE-1 cell NYDKfnhKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1;n[2lvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPFkxQTFizszN NVTncod5W0GQR1XS
human NB10 cell M{PkVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUfJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15Lkm4O|U5KM7:TR?= MnLyV2FPT0WU
human 8505C cell NF\y[mlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHJcohq[mm2aX;uJI9nKGi3bXHuJFg2ODWFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz65PVgyPyEQvF2= NF;idGJUSU6JRWK=
human DB cell MmmyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NW\hcGhuUW6qaXLpeIlwdiCxZjDoeY1idiCGQjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNFEyOzFizszN MmTsV2FPT0WU
human NCI-H28 cell NIL6PWhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3jKRWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPTl6OESg{txO NEK3PHlUSU6JRWK=
human LOXIMVI cell MkTQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MonaTY5pcWKrdHnvckBw\iCqdX3hckBNV1iLTW\JJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE43OzdyMTFOwG0> NVn6enM{W0GQR1XS
human ME-180 cell M2jRfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn:zTY5pcWKrdHnvckBw\iCqdX3hckBOTS1zOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlgxOzl3IN88US=> M3;6VHNCVkeHUh?=
human ES3 cell M2fzOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlywTY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkGyO|k1KM7:TR?= MWrTRW5ITVJ?
human SK-N-FI cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFHIPHpKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjRzNkm5JO69VQ>? NGXITVlUSU6JRWK=
human NBsusSR cell NETkXIhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mlv3TY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOjNzODFOwG0> M{LBfXNCVkeHUh?=
human MCF7 cell MlPES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVfJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6xPFQ6KM7:TR?= NHjobmtUSU6JRWK=
human A549 cell M1LMcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVHJcohq[mm2aX;uJI9nKGi3bXHuJGE2PDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yNFYh|ryP NYq4bmhRW0GQR1XS
human HuP-T4 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoPqTY5pcWKrdHnvckBw\iCqdX3hckBJfVBvVESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE41OjdzIN88US=> NYjEWJZbW0GQR1XS
human HSC-2 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWXGdmllUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNDd6NEKg{txO MoLvV2FPT0WU
human A253 cell NV33S4pxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXTJcohq[mm2aX;uJI9nKGi3bXHuJGEzPTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zNT6yPFM1KM7:TR?= MYjTRW5ITVJ?
human HCT-116 cell NEXCbZRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEXGd5hKdmirYnn0bY9vKG:oIHj1cYFvKFCFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE4xODR|IN88US=> M3\hdnNCVkeHUh?=
human HCT-116 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MojlTY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVkvPjZ6IN88US=> M4XGSnNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID