Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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Cited by 30 Publications

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MnLGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXLWW2JtUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtV25WNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkezNFk2KM7:TR?= NGL6eWJUSU6JRWK=
human H460a cells M{mzS3Bzd2yrZnXyZZRqd25iYYPzZZk> NX\HUnJ1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYx[SClZXzsd{whUUN3ME2xMlA1KM7:TR?= MYixO|MxOzR{MR?=
human MDA435 cells M3\QbXBzd2yrZnXyZZRqd25iYYPzZZk> MlO4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFG0N|Uh[2WubIOsJGlEPTB;MT6xOEDPxE1? M1jjZlE4OzB|NEKx
human HCT116 cells NWi1cmdvWHKxbHnm[ZJifGmxbjDhd5NigQ>? MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczygTWM2OD1zLkG0JO69VQ>? NWji[mU4OTd|MEO0NlE>
human H4 cell M3XLRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml\CTY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUW4O|Uh|ryP MX3TRW5ITVJ?
human PA-1 cell M1\1RWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnfCTY5pcWKrdHnvckBw\iCqdX3hckBRSS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yNlY2PCEQvF2= MlL1V2FPT0WU
human T98G cell NITaSZRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGLuTXdKdmirYnn0bY9vKG:oIHj1cYFvKFR7OFegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlM3QDF{IN88US=> NGS0ZmFUSU6JRWK=
human SW1710 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlE1ODhizszN NGjDRXJUSU6JRWK=
human U251 cell NF;lTWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn;qTY5pcWKrdHnvckBw\iCqdX3hckBWOjVzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|c4OSEQvF2= M1rlbXNCVkeHUh?=
human ALL-PO cell M1fCbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUn1SoxVUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTFwOUC4NVMh|ryP M3vMO3NCVkeHUh?=
human RKO cells MlLaVJJwdGmoZYLheIlwdiCjc4PhfS=> NWDJXnRnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOsJGlEPTB;MjFOwG0> MoHoNVc{ODN2MkG=
human SK-N-DZ cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2XabWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODF6N{[g{txO MnrVV2FPT0WU
human A4-Fuk cell M2PYT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NY\afIg1UW6qaXLpeIlwdiCxZjDoeY1idiCDND3GeYsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB3NkC0JO69VQ>? NFTrU3RUSU6JRWK=
human SW480 cells MU\Qdo9tcW[ncnH0bY9vKGG|c3H5 M125SGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOsJGlEPTB;Mj6yJO69VQ>? NXG3NmxxOTd|MEO0NlE>
human 639-V cell M1fhO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYXvfYdDUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOjd3M{eg{txO M2S2[HNCVkeHUh?=
human Hs-578-T cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2nY[WlvcGmkaYTpc44hd2ZiaIXtZY4hUHNvNUe4MXQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjJ5N{m5JO69VQ>? NYPFVJA2W0GQR1XS
human UM-UC-3 cell MknVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmXQTY5pcWKrdHnvckBw\iCqdX3hckBWVS2XQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0zNjd|OUO2JO69VQ>? M2HkfnNCVkeHUh?=
human BPH-1 cell NHnHXm5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmLyTY5pcWKrdHnvckBw\iCqdX3hckBDWEhvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuPVc1PTVizszN NEjq[o1USU6JRWK=
human 5637 cell M2PNd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2nKdGlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFg2OzJizszN MoHjV2FPT0WU
human 8-MG-BA cell NHuyNY5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4DmdWlvcGmkaYTpc44hd2ZiaIXtZY4hQC2PRz3CRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oy5zNEC0PUDPxE1? M4LRS3NCVkeHUh?=
human HCT116 cells M4nmUmN6fG:2b4jpZ:Kh[XO|YYm= NF\5[3I2KGSjeYO= NXfD[5B[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDViZHH5d{BjgSCPVGOgZZN{[XoxvJygTWM2OD1|LkKg{txO M3vLbVIzOzJ4MU[4
human LB1047-RCC cell MmX4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWXJcohq[mm2aX;uJI9nKGi3bXHuJGxDOTB2Nz3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjJ|NUCxJO69VQ>? MXLTRW5ITVJ?
human KOSC-2 cell NVTlW3FET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGSzTIVKdmirYnn0bY9vKG:oIHj1cYFvKEuRU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDR4Nk[yJO69VQ>? Mlf6V2FPT0WU
human COR-L88 cell NVjiUJAyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHnZPVhKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR5NUizJO69VQ>? NXv6dmdpW0GQR1XS
human PSN1 cell MoThS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3\LRWlvcGmkaYTpc44hd2ZiaIXtZY4hWFOQMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNVA5ODVizszN MV3TRW5ITVJ?
human LXF-289 cell NGPISlBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWHXfJhZUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5{MUe2JO69VQ>? MYjTRW5ITVJ?
human MN-60 cell M1XYWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVTJcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= NHzScIxUSU6JRWK=
human NEC8 cell M1\XVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXPJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12LkS3NFc3KM7:TR?= NX;6eYxLW0GQR1XS
human SJSA1 cells Mln6VJJwdGmoZYLheIlwdiCjc4PhfS=> MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOMU1GxJINmdGy|LDDJR|UxRTRwNUWg{txO NWTMOmllOTd|MEO0NlE>
human NCI-H2405 cell M{nodGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXjJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkSwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzR2OUOg{txO NEDBRZJUSU6JRWK=
human YH-13 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoXLTY5pcWKrdHnvckBw\iCqdX3hckB[UC1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuO|U4PzZizszN MUDTRW5ITVJ?
human SK-MEL-2 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW\sUoF3UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwMEm2N|Ih|pyv MVrTRW5ITVJ?
human NB13 cell NHzOWYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIjXO|BKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> NGrXbGJUSU6JRWK=
human GAMG cell MmC5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUSzNJlRUW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{PzF3IN88US=> MorJV2FPT0WU
human NCI-H1792 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHzpfZRKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc6OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwNEe5OVQh|ryP MkPlV2FPT0WU
human KP-4 cell M2PldWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV3rc4dYUW6qaXLpeIlwdiCxZjDoeY1idiCNUD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QTh{MzFOwG0> M1yxOXNCVkeHUh?=
human HGC-27 cell NG\jNnRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVnJcohq[mm2aX;uJI9nKGi3bXHuJGhISy1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuO|MxPzdizszN NXvxb4ZIW0GQR1XS
human GCT cell M170N2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1TD[WlvcGmkaYTpc44hd2ZiaIXtZY4hT0OWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT64OVEzOSEQvF2= NFTJR41USU6JRWK=
human PFSK-1 cell NXX5W|U2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWjvO3lzUW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njh4MU[4JO69VQ>? MlXUV2FPT0WU
human A172 cell NVPESHg3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1mxdGlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVA4QSEQvF2= MVzTRW5ITVJ?
human HO-1-N-1 cell M4TCVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF;FO2JKdmirYnn0bY9vKG:oIHj1cYFvKEiRLUGtUk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57NUSyOUDPxE1? NGrTU5dUSU6JRWK=
human HN cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXfOPW9DUW6qaXLpeIlwdiCxZjDoeY1idiCKTjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVcxQTdizszN NVqwdWxIW0GQR1XS
human IST-MES1 cell M1LiUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUSyOVRLUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StUWVUOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUe1NlEh|ryP NYrN[IdzW0GQR1XS
human TGW cell Mln5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NW\HfFZkUW6qaXLpeIlwdiCxZjDoeY1idiCWR2egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2Pjh{IN88US=> NEPwS3hUSU6JRWK=
human COR-L23 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlHSTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEKzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{PzN3IN88US=> NX\lSJhKW0GQR1XS
human D-566MG cell M3HhWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HuUWlvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvPzh6N{Og{txO MoX2V2FPT0WU
human SKG-IIIa cell NHnxfoZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGKwTY5KdmirYnn0bY9vKG:oIHj1cYFvKFONRz3JTWliKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi56NUW3NkDPxE1? NVKzWnJSW0GQR1XS
human NCI-H446 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXXFT4s2UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFQ1PiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEG4NUDPxE1? MlnKV2FPT0WU
human KYSE-270 cell NXXTbY0xT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXrJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOzh5MjFOwG0> MnPGV2FPT0WU
human CAL-39 cell NHrvU3NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYPTcGdLUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytN|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjN3MkSg{txO NFvoPYlUSU6JRWK=
human IST-SL1 cell M3m2UWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFzQRo9KdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjR|M{S5JO69VQ>? MkPPV2FPT0WU
human SF539 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnyzTY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuOVUyQTdizszN MYPTRW5ITVJ?
human TE-1 cell MnTaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmG3TY5pcWKrdHnvckBw\iCqdX3hckBVTS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz64PVA6OSEQvF2= MYTTRW5ITVJ?
human NB10 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEnHUFZKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlk5PzV6IN88US=> NXjIbnd6W0GQR1XS
human 8505C cell Mn;kS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF;YNHdKdmirYnn0bY9vKG:oIHj1cYFvKDh3MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QThzNzFOwG0> MX7TRW5ITVJ?
human DB cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYfJcohq[mm2aX;uJI9nKGi3bXHuJGRDKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5yMUGzNUDPxE1? MWTTRW5ITVJ?
human NCI-H28 cell MmrTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXzJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlU6QDh2IN88US=> MnruV2FPT0WU
human LOXIMVI cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1jmeWlvcGmkaYTpc44hd2ZiaIXtZY4hVE:[SV3WTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPjN5MEGg{txO MWjTRW5ITVJ?
human ME-180 cell MnnQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mn3FTY5pcWKrdHnvckBw\iCqdX3hckBOTS1zOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlgxOzl3IN88US=> M3jNWnNCVkeHUh?=
human ES3 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnPMTY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkGyO|k1KM7:TR?= NEHaWWRUSU6JRWK=
human SK-N-FI cell NHXoV2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVTveXNRUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWZKKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS52MU[5PUDPxE1? NVPXcYk1W0GQR1XS
human NBsusSR cell NX:3WGtNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFf2UIpKdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5{M{G4JO69VQ>? M3nuc3NCVkeHUh?=
human MCF7 cell NV;4W24{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVrYZmR7UW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTh2OTFOwG0> Mn;iV2FPT0WU
human A549 cell Mon2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUfORnJkUW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB4IN88US=> M{Ly[XNCVkeHUh?=
human HuP-T4 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVzWTHVIUW6qaXLpeIlwdiCxZjDoeY1idiCKdWCtWFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC52MkexJO69VQ>? NY\RSINzW0GQR1XS
human HSC-2 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVm5ZmxvUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNDd6NEKg{txO M3;uVXNCVkeHUh?=
human A253 cell MnzkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NInNflhKdmirYnn0bY9vKG:oIHj1cYFvKEF{NUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4zQDN2IN88US=> NVn6dms1W0GQR1XS
human HCT-116 cell Mlr2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEfJ[2JKdmirYnn0bY9vKG:oIHj1cYFvKFCFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE4xODR|IN88US=> MYPTRW5ITVJ?
human HCT-116 cell NIn3fWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXzJcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPU43PjhizszN MoLzV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 for 24 h in indicated dose dependent manner, (F) 10 uM RO-3306 for 12 h up to 48 h. The expression level of the indicated protein and the phosphorylated form of H2A.x and p38 were examined via Western blot analyses.

27385216
Immunofluorescence
PARP1 / Plk1 ; 

PubMed: 28069876     


Representative images of IF staining for PARP1 and Plk1 in 22RV1 cells treated with 0.3 mM mimosine for 20 h, 4 mM hydroxyurea for 24 h, 10 µM RO-3306 for 7 h or 200 ng/ml nocodazole (Noc) for 12 h.

28069876
Growth inhibition assay
Cell viability; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 in indicated time- and dose-dependent manner. Cell viability were measured via crystal violet assay. Results are the means ± S.E. n = 3. 

27385216

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID