Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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Cited by 22 Publications

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHfuTm5KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwN{OwPVUh|ryP Mny4V2FPT0WU
human H460a cells M1\6eXBzd2yrZnXyZZRqd25iYYPzZZk> NFzIcZVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFi0OlBiKGOnbHzzMEBKSzVyPUGuNFQh|ryP NWTFV3J1OTd|MEO0NlE>
human MDA435 cells MlPSVJJwdGmoZYLheIlwdiCjc4PhfS=> M174XmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBOFM2KGOnbHzzMEBKSzVyPUGuNVQh|ryP NUXFO|MxOTd|MEO0NlE>
human HCT116 cells MXLQdo9tcW[ncnH0bY9vKGG|c3H5 Mkn6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOsJGlEPTB;MT6xOEDPxE1? M1\xPVE4OzB|NEKx
human H4 cell M2HXemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVrJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5zNUi3OUDPxE1? NXSwO4NzW0GQR1XS
human PA-1 cell NUSzVVRST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV3se2pSUW6qaXLpeIlwdiCxZjDoeY1idiCSQT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zOjZ3NDFOwG0> MnzJV2FPT0WU
human T98G cell M3LGNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfiUpM{UW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjhzMjFOwG0> NUfDTGtJW0GQR1XS
human SW1710 cell NHGybHlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYTvWWFmUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjZzNEC4JO69VQ>? M1G5R3NCVkeHUh?=
human U251 cell NYTDXlZWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoCxTY5pcWKrdHnvckBw\iCqdX3hckBWOjVzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|c4OSEQvF2= NXfE[Zp3W0GQR1XS
human ALL-PO cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXPJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H589yNKEmFNUC9NU46ODhzMzFOwG0> M2C2NnNCVkeHUh?=
human RKO cells NGq1eJRRem:uaX\ldoF1cW:wIHHzd4F6 NXXNWpJNSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOsJGlEPTB;MjFOwG0> MXKxO|MxOzR{MR?=
human SK-N-DZ cell NF;QXXBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M33IdGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODF6N{[g{txO NEjUR3lUSU6JRWK=
human A4-Fuk cell NXG5d5gzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml[0TY5pcWKrdHnvckBw\iCqdX3hckBCPC2IdXugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlA2PjB2IN88US=> NXrHcmRMW0GQR1XS
human SW480 cells NXXKT2VWWHKxbHnm[ZJifGmxbjDhd5NigQ>? NVPtfGZ6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTW|Q5OCClZXzsd{whUUN3ME2yMlIh|ryP NYHQd|ZkOTd|MEO0NlE>
human 639-V cell Ml;5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{Ly[mlvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlI4PTN5IN88US=> NV3vXHE3W0GQR1XS
human Hs-578-T cell M1;sZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIjqT5lKdmirYnn0bY9vKG:oIHj1cYFvKEi|LUW3PE1VKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5{N{e5PUDPxE1? M{DYR3NCVkeHUh?=
human UM-UC-3 cell MnXsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2DySmlvcGmkaYTpc44hd2ZiaIXtZY4hXU1vVVOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oi55M{mzOkDPxE1? M3fYcnNCVkeHUh?=
human BPH-1 cell NY[0RnZwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXLxfWw2UW6qaXLpeIlwdiCxZjDoeY1idiCEUFitNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTd2NUWg{txO NFvxe5BUSU6JRWK=
human 5637 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1\N[GlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFg2OzJizszN Mle1V2FPT0WU
human 8-MG-BA cell NXLTfHNOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3vzNWlvcGmkaYTpc44hd2ZiaIXtZY4hQC2PRz3CRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oy5zNEC0PUDPxE1? MYXTRW5ITVJ?
human HCT116 cells MXLDfZRwfG:6aXRCpIF{e2G7 MUC1JIRigXN? NHvMO|hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5Nige,:jDDJR|UxRTNwMjFOwG0> NUi0OnRmOjJ|Mk[xOlg>
human LB1047-RCC cell M4XuO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVTJcohq[mm2aX;uJI9nKGi3bXHuJGxDOTB2Nz3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjJ|NUCxJO69VQ>? MYnTRW5ITVJ?
human KOSC-2 cell MorFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MofZTY5pcWKrdHnvckBw\iCqdX3hckBMV1OFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlQ1PjZ4MjFOwG0> MkDKV2FPT0WU
human COR-L88 cell NVHmRmR2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M13EOWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVy4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDd3OEOg{txO NX3oTYw1W0GQR1XS
human PSN1 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlznTY5pcWKrdHnvckBw\iCqdX3hckBRW05zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xNFgxPSEQvF2= M4DIdnNCVkeHUh?=
human LXF-289 cell NW[4PW9FT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlIyPzZizszN MkX6V2FPT0WU
human MN-60 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= NF7YPI1USU6JRWK=
human NEC8 cell NVG0UIJ5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYWyTVFzUW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE41PzB5NjFOwG0> MX;TRW5ITVJ?
human SJSA1 cells NHXsSI9Rem:uaX\ldoF1cW:wIHHzd4F6 M2LYfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1rTRVEh[2WubIOsJGlEPTB;ND61OUDPxE1? M3TyRVE4OzB|NEKx
human NCI-H2405 cell NYHV[3dZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnXnTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK0NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01Njd2NEmzJO69VQ>? M3vSfXNCVkeHUh?=
human YH-13 cell NX;sNI5yT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVjJcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND63OVc4PiEQvF2= MojyV2FPT0WU
human SK-MEL-2 cell NEfMXopIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYPJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4xQTZ|MjFOoI0> M2TNOnNCVkeHUh?=
human NB13 cell NEf6RWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2LITWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|I4QDZizszN MoH2V2FPT0WU
human GAMG cell Mo\BS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4LiRmlvcGmkaYTpc44hd2ZiaIXtZY4hT0GPRzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|cyPSEQvF2= M2D0[HNCVkeHUh?=
human NCI-H1792 cell MkjPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{XzbGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixO|kzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS52N{m1OEDPxE1? NFvTdHpUSU6JRWK=
human KP-4 cell Mln2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoTsTY5pcWKrdHnvckBw\iCqdX3hckBMWC12IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT62PVgzOyEQvF2= NYLrepF7W0GQR1XS
human HGC-27 cell NVHTZ2hLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEfPTY1KdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzNyN{eg{txO M1L5OHNCVkeHUh?=
human GCT cell NHy2SJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXfzVJJtUW6qaXLpeIlwdiCxZjDoeY1idiCJQ2SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlg2OTJzIN88US=> NHvtUmFUSU6JRWK=
human PFSK-1 cell NYWwfpE6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWfJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN NHzuUGhUSU6JRWK=
human A172 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4DXXGlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVA4QSEQvF2= Moi0V2FPT0WU
human HO-1-N-1 cell M4rSWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGhQNTFvTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PTR{NTFOwG0> MUXTRW5ITVJ?
human HN cell NWrIXYVJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3zXTmlvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lkm3NFk4KM7:TR?= NHnES5JUSU6JRWK=
human IST-MES1 cell M3TqXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{TV[mlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLV3FV|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl5NUKxJO69VQ>? MmGxV2FPT0WU
human TGW cell M{H3Xmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NITW[VNKdmirYnn0bY9vKG:oIHj1cYFvKFSJVzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uNFU3QDJizszN NEDEOVRUSU6JRWK=
human COR-L23 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\i[IlKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MNlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjN5M{Wg{txO M3TnWnNCVkeHUh?=
human D-566MG cell M{W0W2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHezRWNKdmirYnn0bY9vKG:oIHj1cYFvKERvNU[2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njd6OEezJO69VQ>? MVTTRW5ITVJ?
human SKG-IIIa cell NIDPS2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4XkeGlvcGmkaYTpc44hd2ZiaIXtZY4hW0uJLVnJTYEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njh3NUeyJO69VQ>? NGLGOFFUSU6JRWK=
human NCI-H446 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGnFOIFKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFQ3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yMUixJO69VQ>? NG\nSIRUSU6JRWK=
human KYSE-270 cell MkLOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Moi5TY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUK3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODN6N{Kg{txO NILmWZhUSU6JRWK=
human CAL-39 cell NVLkcIpUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHTWNWZKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2zPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOzV{NDFOwG0> MWjTRW5ITVJ?
human IST-SL1 cell MnfNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnfWTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{41OzN2OTFOwG0> MXPTRW5ITVJ?
human SF539 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17iXmlvcGmkaYTpc44hd2ZiaIXtZY4hW0Z3M{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlU2OTl5IN88US=> M1HodnNCVkeHUh?=
human TE-1 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1XIRWlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPFkxQTFizszN MlnHV2FPT0WU
human NB10 cell NVHBfW1oT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15Lkm4O|U5KM7:TR?= MkL6V2FPT0WU
human 8505C cell NXe3TI5WT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlvWTY5pcWKrdHnvckBw\iCqdX3hckA5PTB3QzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVk5OTdizszN M37ubHNCVkeHUh?=
human DB cell M4LYNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml;JTY5pcWKrdHnvckBw\iCqdX3hckBFSiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwMEGxN|Eh|ryP NXXDOoFrW0GQR1XS
human NCI-H28 cell M4S0WWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXu3cnY1UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC53OUi4OEDPxE1? MlTqV2FPT0WU
human LOXIMVI cell MmLjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYLJcohq[mm2aX;uJI9nKGi3bXHuJGxQYEmPVlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY{PzBzIN88US=> M{PtOnNCVkeHUh?=
human ME-180 cell NVfNXmYzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlzpTY5pcWKrdHnvckBw\iCqdX3hckBOTS1zOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlgxOzl3IN88US=> NEjHTYJUSU6JRWK=
human ES3 cell MoHOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUS2OWZTUW6qaXLpeIlwdiCxZjDoeY1idiCHU{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlEzPzl2IN88US=> MoDSV2FPT0WU
human SK-N-FI cell NEHmTWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVfJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU5vRlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlQyPjl7IN88US=> NHr6TXVUSU6JRWK=
human NBsusSR cell NF7Ce3NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFn3fpFKdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5{M{G4JO69VQ>? M1rUU3NCVkeHUh?=
human MCF7 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHO3WZNKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> NHz0[lBUSU6JRWK=
human A549 cell NWDSclQ3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NE[3bplKdmirYnn0bY9vKG:oIHj1cYFvKEF3NEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4zODZizszN MWXTRW5ITVJ?
human HuP-T4 cell MmiyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4PyUWlvcGmkaYTpc44hd2ZiaIXtZY4hUHWSLWS0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvPDJ5MTFOwG0> MX\TRW5ITVJ?
human HSC-2 cell NXnx[2VRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGsO|g1OiEQvF2= MkO2V2FPT0WU
human A253 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYKzS5Y6UW6qaXLpeIlwdiCxZjDoeY1idiCDMkWzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh|NDFOwG0> M{jrV3NCVkeHUh?=
human HCT-116 cell NGTyU|ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkjjTY5pcWKrdHnvckBw\iCqdX3hckBRSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUiuNFA1OyEQvF2= MY\TRW5ITVJ?
human HCT-116 cell NEHF[Y1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVvJcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPU43PjhizszN NVTWXFJvW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 for 24 h in indicated dose dependent manner, (F) 10 uM RO-3306 for 12 h up to 48 h. The expression level of the indicated protein and the phosphorylated form of H2A.x and p38 were examined via Western blot analyses.

27385216
Immunofluorescence
PARP1 / Plk1 ; 

PubMed: 28069876     


Representative images of IF staining for PARP1 and Plk1 in 22RV1 cells treated with 0.3 mM mimosine for 20 h, 4 mM hydroxyurea for 24 h, 10 µM RO-3306 for 7 h or 200 ng/ml nocodazole (Noc) for 12 h.

28069876
Growth inhibition assay
Cell viability; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 in indicated time- and dose-dependent manner. Cell viability were measured via crystal violet assay. Results are the means ± S.E. n = 3. 

27385216

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID