Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

    (A). Cell toxicity of CDK1 Inhibitor (RO‐3306) or CDK6 (Palbociclib HCl) on Huh7 cells. (B). Flow cytometry analysis of the cell cycle by CDK1 inhibitor (5 μm) or CDK6 inhibitor (2.5 μm) treatment.

    FEBS Lett, 2018, 592(11):1893-1904. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NWSyXGI1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4XUfGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55M{C5OUDPxE1? MUPTRW5ITVJ?
human H460a cells NGraZ3VRem:uaX\ldoF1cW:wIHHzd4F6 Ml;mRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKNE[wZUBk\WyuczygTWM2OD1zLkC0JO69VQ>? MUSxO|MxOzR{MR?=
human MDA435 cells MnTDVJJwdGmoZYLheIlwdiCjc4PhfS=> NYXFV5dmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGE1OzViY3XscJMtKEmFNUC9NU4yPCEQvF2= MkfTNVc{ODN2MkG=
human HCT116 cells NYLMSlZ6WHKxbHnm[ZJifGmxbjDhd5NigQ>? NEmxU3BCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3ME2xMlE1KM7:TR?= MlTQNVc{ODN2MkG=
human H4 cell MkXiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlvjTY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUW4O|Uh|ryP NWO2cldJW0GQR1XS
human PA-1 cell NXr4dZJNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3PrfmlvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlI3PTRizszN NHv1NnBUSU6JRWK=
human T98G cell NGexWJFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4H4UmlvcGmkaYTpc44hd2ZiaIXtZY4hXDl6RzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|Y5OTJizszN MX7TRW5ITVJ?
human SW1710 cell NXHmZnJUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NG\QPFBKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjF2MEig{txO NH3GfZRUSU6JRWK=
human U251 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV7Efnd6UW6qaXLpeIlwdiCxZjDoeY1idiCXMkWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43Ozd5MTFOwG0> MW\TRW5ITVJ?
human ALL-PO cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn;kTY5pcWKrdHnvckBw\iCqdX3hckBCVExvUF:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVEvQTB6MUOg{txO NIXl[5lUSU6JRWK=
human RKO cells NYDDRpFnWHKxbHnm[ZJifGmxbjDhd5NigQ>? MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyxiSVO1NF0zKM7:TR?= MkPwNVc{ODN2MkG=
human SK-N-DZ cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NITFZmNKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjBzOEe2JO69VQ>? MWDTRW5ITVJ?
human A4-Fuk cell NGLt[m5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3\w[2lvcGmkaYTpc44hd2ZiaIXtZY4hSTRvRoXrJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4xPTZyNDFOwG0> NET0eZJUSU6JRWK=
human SW480 cells NYT0SpVZWHKxbHnm[ZJifGmxbjDhd5NigQ>? M3PEb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOsJGlEPTB;Mj6yJO69VQ>? NUnoR5JROTd|MEO0NlE>
human 639-V cell NHixVldIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV3o[Zl{UW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOjd3M{eg{txO NUHqS3dWW0GQR1XS
human Hs-578-T cell M3i2fWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2\K[2lvcGmkaYTpc44hd2ZiaIXtZY4hUHNvNUe4MXQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjJ5N{m5JO69VQ>? NH;QSXpUSU6JRWK=
human UM-UC-3 cell NVu2bVQ{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVX2[m9kUW6qaXLpeIlwdiCxZjDoeY1idiCXTT3VR{0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1{LkezPVM3KM7:TR?= NUPmb3dFW0GQR1XS
human BPH-1 cell MmTQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVHPS|VEUW6qaXLpeIlwdiCxZjDoeY1idiCEUFitNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTd2NUWg{txO M13HNnNCVkeHUh?=
human 5637 cell M3;T[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4rVe2lvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFg2OzJizszN MnnSV2FPT0WU
human 8-MG-BA cell MmD3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJFguVUdvQlGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVMvOTRyNEmg{txO NV\sW4xjW0GQR1XS
human HCT116 cells M33o[mN6fG:2b4jpZ:Kh[XO|YYm= M3rZdFUh\GG7cx?= MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOUBl[Xm|IHL5JG1VWyCjc4Phfg+9lCCLQ{WwQVMvOiEQvF2= NXXpUnZCOjJ|Mk[xOlg>
human LB1047-RCC cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFyyfHlKdmirYnn0bY9vKG:oIHj1cYFvKEyEMUC0O{1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkKzOVAyKM7:TR?= NV7pO5A3W0GQR1XS
human KOSC-2 cell M1fLVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1\KdGlvcGmkaYTpc44hd2ZiaIXtZY4hU0:VQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41PDZ4NkKg{txO MnTDV2FPT0WU
human COR-L88 cell NXHqUI4xT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFj2OHlKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR5NUizJO69VQ>? MnO2V2FPT0WU
human PSN1 cell NUHSUJhMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoSzTY5pcWKrdHnvckBw\iCqdX3hckBRW05zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xNFgxPSEQvF2= NEPmNo9USU6JRWK=
human LXF-289 cell M2HBSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYfJcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlIyPzZizszN NYrZUHlSW0GQR1XS
human MN-60 cell NWfjdZJrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXjJcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= NU\sTFJFW0GQR1XS
human NEC8 cell NVv2ZVZCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkSzTY5pcWKrdHnvckBw\iCqdX3hckBPTUN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND60O|A4PiEQvF2= MlzTV2FPT0WU
human SJSA1 cells MY\Qdo9tcW[ncnH0bY9vKGG|c3H5 NFq3dHlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPKV2EyKGOnbHzzMEBKSzVyPUSuOVUh|ryP NHvy[ZoyPzNyM{SyNS=>
human NCI-H2405 cell MmnhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWG2bHp5UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI1ODViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lke0OFk{KM7:TR?= M2XmWnNCVkeHUh?=
human YH-13 cell NWL0PYtHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlfJTY5pcWKrdHnvckBw\iCqdX3hckB[UC1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuO|U4PzZizszN NYq0cpl{W0GQR1XS
human SK-MEL-2 cell NYXqZYdnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2XiRWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjB7NkOyJO6ddQ>? NXj1OVJqW0GQR1XS
human NB13 cell NVzBcpFNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoroTY5pcWKrdHnvckBw\iCqdX3hckBPSjF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zNlc5PiEQvF2= MlXiV2FPT0WU
human GAMG cell NFnLcmVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVPGbHBmUW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{PzF3IN88US=> NX[1WG01W0GQR1XS
human NCI-H1792 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPDd7NUSg{txO NVi3b2ZkW0GQR1XS
human KP-4 cell NYPSdFhtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFzTNJhKdmirYnn0bY9vKG:oIHj1cYFvKEuSLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlY6QDJ|IN88US=> M1P4bnNCVkeHUh?=
human HGC-27 cell M1;xcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIfDfG1KdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzNyN{eg{txO NF7NcVJUSU6JRWK=
human GCT cell NGroTYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmruTY5pcWKrdHnvckBw\iCqdX3hckBIS1RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lki1NVIyKM7:TR?= MVLTRW5ITVJ?
human PFSK-1 cell NH3SWINIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN MUDTRW5ITVJ?
human A172 cell NH3Td25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzaTY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT65NFc6KM7:TR?= M3XOXXNCVkeHUh?=
human HO-1-N-1 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MojhTY5pcWKrdHnvckBw\iCqdX3hckBJVy1zLV6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTV2MkWg{txO NFvVWnRUSU6JRWK=
human HN cell MkOxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWnQcGZLUW6qaXLpeIlwdiCxZjDoeY1idiCKTjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVcxQTdizszN M3X6OXNCVkeHUh?=
human IST-MES1 cell NEDk[INIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHjGSolKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3NSXMyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{WyNUDPxE1? MXfTRW5ITVJ?
human TGW cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NE\3cFBKdmirYnn0bY9vKG:oIHj1cYFvKFSJVzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uNFU3QDJizszN MnO3V2FPT0WU
human COR-L23 cell MnX2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUP3Z3hbUW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFI{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi5|N{O1JO69VQ>? MX3TRW5ITVJ?
human D-566MG cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1j3WmlvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvPzh6N{Og{txO Mm\IV2FPT0WU
human SKG-IIIa cell MlvOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX;PSZRwUW6qaXLpeIlwdiCxZjDoeY1idiCVS1etTWlK[SClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwOEW1O|Ih|ryP M134fHNCVkeHUh?=
human NCI-H446 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4fOWmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OFYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjBzOEGg{txO NXLwbpc4W0GQR1XS
human KYSE-270 cell M{i5Smdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mnu4TY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUK3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODN6N{Kg{txO M4nrUXNCVkeHUh?=
human CAL-39 cell M{XROmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnTFTY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlM2OjRizszN MoX0V2FPT0WU
human IST-SL1 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYn3ZWR6UW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py52M{O0PUDPxE1? MoP2V2FPT0WU
human SF539 cell M1z2SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Moj2TY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuOVUyQTdizszN MV;TRW5ITVJ?
human TE-1 cell NGXSOXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mor4TY5pcWKrdHnvckBw\iCqdX3hckBVTS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz64PVA6OSEQvF2= NYT6OGFwW0GQR1XS
human NB10 cell Mn3FS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{PYbmlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVg4PThizszN M2DBSHNCVkeHUh?=
human 8505C cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M13GOWlvcGmkaYTpc44hd2ZiaIXtZY4hQDVyNVOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlk6QDF5IN88US=> NV7EephVW0GQR1XS
human DB cell M4jVfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnjyTY5pcWKrdHnvckBw\iCqdX3hckBFSiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwMEGxN|Eh|ryP MkjSV2FPT0WU
human NCI-H28 cell NI\kfWhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmnnTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42QTh6NDFOwG0> NWLMTpBVW0GQR1XS
human LOXIMVI cell M1LuZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFLEbVNKdmirYnn0bY9vKG:oIHj1cYFvKEyRWFnNWmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjZ|N{CxJO69VQ>? MmKzV2FPT0WU
human ME-180 cell M124fGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWXVTGJlUW6qaXLpeIlwdiCxZjDoeY1idiCPRT2xPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjhyM{m1JO69VQ>? M1OzdXNCVkeHUh?=
human ES3 cell MmnuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWPJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTlwMUK3PVQh|ryP NV;GUGV5W0GQR1XS
human SK-N-FI cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknsTY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLV\JJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU41OTZ7OTFOwG0> M3XRTXNCVkeHUh?=
human NBsusSR cell NX7yWZpET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWjRemYyUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwMkOxPEDPxE1? MVfTRW5ITVJ?
human MCF7 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWXWZlUxUW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTh2OTFOwG0> NFjTTVlUSU6JRWK=
human A549 cell M363WWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGE2PDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yNFYh|ryP NGnWVGJUSU6JRWK=
human HuP-T4 cell NF7WW2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWDJcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlQzPzFizszN MV;TRW5ITVJ?
human HSC-2 cell NVrGS4t2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkDITY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMFc5PDJizszN M4e2dnNCVkeHUh?=
human A253 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1rreWlvcGmkaYTpc44hd2ZiaIXtZY4hSTJ3MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG1MlI5OzRizszN M2CxT3NCVkeHUh?=
human HCT-116 cell M3TQe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1XCZWlvcGmkaYTpc44hd2ZiaIXtZY4hWENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlAxPDNizszN MX;TRW5ITVJ?
human HCT-116 cell MnTGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{nZbmlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUGxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE6NjZ4ODFOwG0> NIG3eIdUSU6JRWK=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID