Ro-3306

For research use only. Not for use in humans.

Catalog No.S7747

31 publications

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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Selleck's Ro-3306 has been cited by 31 publications

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MoHSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnTXTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvU17VMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd|MEm1JO69VQ>? NFWzdWpUSU6JRWK=
human H460a cells MoXOVJJwdGmoZYLheIlwdiCjc4PhfS=> NXHJT3pYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYx[SClZXzsd{whUUN3ME2xMlA1KM7:TR?= NH:zVWkyPzNyM{SyNS=>
human MDA435 cells MoD3VJJwdGmoZYLheIlwdiCjc4PhfS=> MnSxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFG0N|Uh[2WubIOsJGlEPTB;MT6xOEDPxE1? MlHoNVc{ODN2MkG=
human HCT116 cells M1rFe3Bzd2yrZnXyZZRqd25iYYPzZZk> M1fSUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzMEBKSzVyPUGuNVQh|ryP NFvGV5EyPzNyM{SyNS=>
human H4 cell M1jxO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWfJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5zNUi3OUDPxE1? NFfLb3VUSU6JRWK=
human PA-1 cell NGfBTZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHPiN3NKdmirYnn0bY9vKG:oIHj1cYFvKFCDLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIzPjV2IN88US=> MXvTRW5ITVJ?
human T98G cell M1LWT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXTjRXNYUW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjhzMjFOwG0> MUnTRW5ITVJ?
human SW1710 cell NXjRPGZOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVzielJCUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjZzNEC4JO69VQ>? Mn7QV2FPT0WU
human U251 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mli0TY5pcWKrdHnvckBw\iCqdX3hckBWOjVzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|c4OSEQvF2= M{HvVHNCVkeHUh?=
human ALL-PO cell NELjTVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3;PbmlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0yNjlyOEGzJO69VQ>? M4jPXXNCVkeHUh?=
human RKO cells MkD3VJJwdGmoZYLheIlwdiCjc4PhfS=> NHnrUnhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLLU{Bk\WyuczygTWM2OD1{IN88US=> MUOxO|MxOzR{MR?=
human SK-N-DZ cell M3jvUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGDZOnBKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjBzOEe2JO69VQ>? MWHTRW5ITVJ?
human A4-Fuk cell M1fiRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGD1ZWdKdmirYnn0bY9vKG:oIHj1cYFvKEF2LV\1b{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODV4MESg{txO Ml\4V2FPT0WU
human SW480 cells NFTqNINRem:uaX\ldoF1cW:wIHHzd4F6 Ml:2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczygTWM2OD1{LkKg{txO NHTY[lUyPzNyM{SyNS=>
human 639-V cell NGXqOpVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlvMTY5pcWKrdHnvckBw\iCqdX3hckA3OzlvVjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNlc2OzdizszN MXfTRW5ITVJ?
human Hs-578-T cell MlXoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXrzUnQyUW6qaXLpeIlwdiCxZjDoeY1idiCKcz21O|guXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMke3PVkh|ryP MmDEV2FPT0WU
human UM-UC-3 cell NEH1WmZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoPWTY5pcWKrdHnvckBw\iCqdX3hckBWVS2XQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0zNjd|OUO2JO69VQ>? NVvq[nRNW0GQR1XS
human BPH-1 cell Mo\nS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml:yTY5pcWKrdHnvckBw\iCqdX3hckBDWEhvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuPVc1PTVizszN NU[2eopZW0GQR1XS
human 5637 cell M37xOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1;rZ2lvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFg2OzJizszN M1HjSXNCVkeHUh?=
human 8-MG-BA cell NELJbHlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYDJcohq[mm2aX;uJI9nKGi3bXHuJFguVUdvQlGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVMvOTRyNEmg{txO MYjTRW5ITVJ?
human HCT116 cells M4PE[mN6fG:2b4jpZ:Kh[XO|YYm= NHfzNpk2KGSjeYO= M1XDSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjZoTldkA2KGSjeYOgZpkhVVSVIHHzd4F697zOIFnDOVA:Oy5{IN88US=> NX7OSpRrOjJ|Mk[xOlg>
human LB1047-RCC cell Mn2wS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYLFT|JKUW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yN|UxOSEQvF2= MVXTRW5ITVJ?
human KOSC-2 cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV;NbpVWUW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR2Nk[2NkDPxE1? MUPTRW5ITVJ?
human COR-L88 cell M3PlTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXjm[XhRUW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFg5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy52N{W4N{DPxE1? NE\jTZRUSU6JRWK=
human PSN1 cell NVrYfZliT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn7kTY5pcWKrdHnvckBw\iCqdX3hckBRW05zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xNFgxPSEQvF2= NVfnbGMxW0GQR1XS
human LXF-289 cell MoGwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NG\ReHFKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01NjJzN{[g{txO NV3MXZRbW0GQR1XS
human MN-60 cell Ml;PS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYTJcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= MWrTRW5ITVJ?
human NEC8 cell NIDkWXZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4jrOmlvcGmkaYTpc44hd2ZiaIXtZY4hVkWFODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFcxPzZizszN MVnTRW5ITVJ?
human SJSA1 cells NHzCN|ZRem:uaX\ldoF1cW:wIHHzd4F6 NXT6cWNISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTTnNCOSClZXzsd{whUUN3ME20MlU2KM7:TR?= MlzENVc{ODN2MkG=
human NCI-H2405 cell Moj2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHPtZ3VKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlQxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwN{S0PVMh|ryP M4fSTHNCVkeHUh?=
human YH-13 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4m4XGlvcGmkaYTpc44hd2ZiaIXtZY4hYUhvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20Mlc2Pzd4IN88US=> MnOwV2FPT0WU
human SK-MEL-2 cell NVfrfpRIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFTN[XhKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS5yOU[zNkDPpG1? MV;TRW5ITVJ?
human NB13 cell M2Pj[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{LOOGlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|I4QDZizszN MUjTRW5ITVJ?
human GAMG cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlPBTY5pcWKrdHnvckBw\iCqdX3hckBISU2JIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zO|E2KM7:TR?= NFj4eXpUSU6JRWK=
human NCI-H1792 cell NXnE[|Q{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVq3XpFRUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE4QTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkS3PVU1KM7:TR?= MX3TRW5ITVJ?
human KP-4 cell NUD3XVVkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX3nUXpNUW6qaXLpeIlwdiCxZjDoeY1idiCNUD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QTh{MzFOwG0> NFy3W3VUSU6JRWK=
human HGC-27 cell NInHbWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mmj5TY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlc{ODd5IN88US=> M2rmTHNCVkeHUh?=
human GCT cell MnL3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlPxTY5pcWKrdHnvckBw\iCqdX3hckBIS1RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lki1NVIyKM7:TR?= MlXTV2FPT0WU
human PFSK-1 cell M3zTPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX\Jcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN NGezPXlUSU6JRWK=
human A172 cell M1PWTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NG\vVmdKdmirYnn0bY9vKG:oIHj1cYFvKEFzN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlkxPzlizszN NVHVXXBuW0GQR1XS
human HO-1-N-1 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIr5NGRKdmirYnn0bY9vKG:oIHj1cYFvKEiRLUGtUk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57NUSyOUDPxE1? M2TIXnNCVkeHUh?=
human HN cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGhPKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{C5O{DPxE1? NHXrXYhUSU6JRWK=
human IST-MES1 cell NYXuWJlMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEDiZoNKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3NSXMyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{WyNUDPxE1? MV3TRW5ITVJ?
human TGW cell M3XkcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGroV3pKdmirYnn0bY9vKG:oIHj1cYFvKFSJVzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uNFU3QDJizszN NGDubmNUSU6JRWK=
human COR-L23 cell MlmxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1O3[WlvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVyyN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvOzd|NTFOwG0> NIX3UJhUSU6JRWK=
human D-566MG cell MkX0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXTJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTZ4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22Mlc5QDd|IN88US=> MYXTRW5ITVJ?
human SKG-IIIa cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXvjWGk6UW6qaXLpeIlwdiCxZjDoeY1idiCVS1etTWlK[SClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwOEW1O|Ih|ryP Mn21V2FPT0WU
human NCI-H446 cell NEn3dVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXrWVZBSUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFQ1PiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEG4NUDPxE1? M{\zdXNCVkeHUh?=
human KYSE-270 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYPrZo5WUW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEO4O|Ih|ryP MY\TRW5ITVJ?
human CAL-39 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2nXeGlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4{PTJ2IN88US=> MmTSV2FPT0WU
human IST-SL1 cell NXPkeVJQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWjJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ{OzR7IN88US=> M17FZXNCVkeHUh?=
human SF539 cell M3;VV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVrJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz61OVE6PyEQvF2= NI\CR2VUSU6JRWK=
human TE-1 cell M{HuOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1zr[mlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPFkxQTFizszN MXXTRW5ITVJ?
human NB10 cell NYHzXYdqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3\1[mlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVg4PThizszN NEXkRYpUSU6JRWK=
human 8505C cell NHSydXdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2jDWWlvcGmkaYTpc44hd2ZiaIXtZY4hQDVyNVOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlk6QDF5IN88US=> NVj1b2s2W0GQR1XS
human DB cell NH;hbVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWXJcohq[mm2aX;uJI9nKGi3bXHuJGRDKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5yMUGzNUDPxE1? NHnEcW5USU6JRWK=
human NCI-H28 cell NYjmUmh6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmiyTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42QTh6NDFOwG0> NGfVfW9USU6JRWK=
human LOXIMVI cell M4nMc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MljkTY5pcWKrdHnvckBw\iCqdX3hckBNV1iLTW\JJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE43OzdyMTFOwG0> MVXTRW5ITVJ?
human ME-180 cell M3jNOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYK2V|lrUW6qaXLpeIlwdiCxZjDoeY1idiCPRT2xPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjhyM{m1JO69VQ>? NGPseHNUSU6JRWK=
human ES3 cell NGTifIJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGDYbYpKdmirYnn0bY9vKG:oIHj1cYFvKEWVMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuNVI4QTRizszN NX[yTFJqW0GQR1XS
human SK-N-FI cell Ml[0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYnJepFYUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWZKKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS52MU[5PUDPxE1? MnTyV2FPT0WU
human NBsusSR cell NYTmXmZ1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NILz[HBKdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5{M{G4JO69VQ>? M{LFXXNCVkeHUh?=
human MCF7 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHLOVYZKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> Mn:1V2FPT0WU
human A549 cell NULSO5R1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWX3boVmUW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB4IN88US=> NYLVTGhXW0GQR1XS
human HuP-T4 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlQzPzFizszN NFXQPFBUSU6JRWK=
human HSC-2 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NU\IZnl7UW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNDd6NEKg{txO NEHZTWRUSU6JRWK=
human A253 cell Mli1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWfrNJNQUW6qaXLpeIlwdiCxZjDoeY1idiCDMkWzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh|NDFOwG0> NXfWTplRW0GQR1XS
human HCT-116 cell NWCxZ5hLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1y3emlvcGmkaYTpc44hd2ZiaIXtZY4hWENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlAxPDNizszN MkKyV2FPT0WU
human HCT-116 cell NGPKXYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2TBXmlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUGxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE6NjZ4ODFOwG0> MVnTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 for 24 h in indicated dose dependent manner, (F) 10 uM RO-3306 for 12 h up to 48 h. The expression level of the indicated protein and the phosphorylated form of H2A.x and p38 were examined via Western blot analyses.

27385216
Immunofluorescence
PARP1 / Plk1 ; 

PubMed: 28069876     


Representative images of IF staining for PARP1 and Plk1 in 22RV1 cells treated with 0.3 mM mimosine for 20 h, 4 mM hydroxyurea for 24 h, 10 µM RO-3306 for 7 h or 200 ng/ml nocodazole (Noc) for 12 h.

28069876
Growth inhibition assay
Cell viability; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 in indicated time- and dose-dependent manner. Cell viability were measured via crystal violet assay. Results are the means ± S.E. n = 3. 

27385216

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A
Smiles O=C1N=C(NCC2=CC=CS2)S\C1=C/C3=CC=C4N=CC=CC4=C3

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID