Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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Cited by 9 Publications

4 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

    (A). Cell toxicity of CDK1 Inhibitor (RO‐3306) or CDK6 (Palbociclib HCl) on Huh7 cells. (B). Flow cytometry analysis of the cell cycle by CDK1 inhibitor (5 μm) or CDK6 inhibitor (2.5 μm) treatment.

    FEBS Lett, 2018, 592(11):1893-1904. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NHr4eXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmfiTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvU17VMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd|MEm1JO69VQ>? MX\TRW5ITVJ?
human H460a cells M3myR3Bzd2yrZnXyZZRqd25iYYPzZZk> M2f5OWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSES2NIEh[2WubIOsJGlEPTB;MT6wOEDPxE1? NF7v[YIyPzNyM{SyNS=>
human MDA435 cells MkHuVJJwdGmoZYLheIlwdiCjc4PhfS=> MlXWRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFG0N|Uh[2WubIOsJGlEPTB;MT6xOEDPxE1? MX[xO|MxOzR{MR?=
human HCT116 cells NHTrSVJRem:uaX\ldoF1cW:wIHHzd4F6 MmjoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOsJGlEPTB;MT6xOEDPxE1? M4DsXVE4OzB|NEKx
human H4 cell M3PzcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5zNUi3OUDPxE1? M4e5cnNCVkeHUh?=
human PA-1 cell MnnYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFLuNGVKdmirYnn0bY9vKG:oIHj1cYFvKFCDLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIzPjV2IN88US=> MWTTRW5ITVJ?
human T98G cell NITvdZRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYTJcohq[mm2aX;uJI9nKGi3bXHuJHQ6QEdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkO2PFEzKM7:TR?= NG\uRlZUSU6JRWK=
human SW1710 cell NFLWPJlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWXaToFKUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjZzNEC4JO69VQ>? M2rI[3NCVkeHUh?=
human U251 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJHUzPTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLk[zO|cyKM7:TR?= M4PNZnNCVkeHUh?=
human ALL-PO cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2fIOmlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0yNjlyOEGzJO69VQ>? NEXO[IdUSU6JRWK=
human RKO cells NGfJR3pRem:uaX\ldoF1cW:wIHHzd4F6 NWjZTWhSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOsJGlEPTB;MjFOwG0> Mn;NNVc{ODN2MkG=
human SK-N-DZ cell NFvufYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF7jPY9KdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjBzOEe2JO69VQ>? NFuwN3ZUSU6JRWK=
human A4-Fuk cell NYH5fZlbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVnDW|Y{UW6qaXLpeIlwdiCxZjDoeY1idiCDND3GeYsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB3NkC0JO69VQ>? MnL0V2FPT0WU
human SW480 cells NH7KXGJRem:uaX\ldoF1cW:wIHHzd4F6 NHf3NmpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPXOFgxKGOnbHzzMEBKSzVyPUKuNkDPxE1? NETLc|cyPzNyM{SyNS=>
human 639-V cell NXr1XY9KT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mk\3TY5pcWKrdHnvckBw\iCqdX3hckA3OzlvVjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNlc2OzdizszN NF2yOZZUSU6JRWK=
human Hs-578-T cell Mn;6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGh{NTV5OD3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4zPzd7OTFOwG0> M2PJUHNCVkeHUh?=
human UM-UC-3 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4q4XmlvcGmkaYTpc44hd2ZiaIXtZY4hXU1vVVOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oi55M{mzOkDPxE1? MojDV2FPT0WU
human BPH-1 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUnufllUUW6qaXLpeIlwdiCxZjDoeY1idiCEUFitNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTd2NUWg{txO MljrV2FPT0WU
human 5637 cell NE\Xd21Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2jYSGlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFg2OzJizszN NV63XIVqW0GQR1XS
human 8-MG-BA cell NF7mOFZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFSzbIlKdmirYnn0bY9vKG:oIHj1cYFvKDhvTVetRmEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTNwMUSwOFkh|ryP M2f3cnNCVkeHUh?=
human HCT116 cells Ml7rR5l1d3SxeHnjxsBie3OjeR?= NGO3SIs2KGSjeYO= M1zmeWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjZoTldkA2KGSjeYOgZpkhVVSVIHHzd4F697zOIFnDOVA:Oy5{IN88US=> NW[2OWI{OjJ|Mk[xOlg>
human LB1047-RCC cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVfJcohq[mm2aX;uJI9nKGi3bXHuJGxDOTB2Nz3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjJ|NUCxJO69VQ>? NVjUUVkzW0GQR1XS
human KOSC-2 cell Ml7ES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF3RSoxKdmirYnn0bY9vKG:oIHj1cYFvKEuRU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDR4Nk[yJO69VQ>? M1\Hd3NCVkeHUh?=
human COR-L88 cell MkjES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3i1TmlvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVy4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDd3OEOg{txO MnzYV2FPT0WU
human PSN1 cell NUjqPFhkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV;HTYw2UW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yODhyNTFOwG0> MVTTRW5ITVJ?
human LXF-289 cell NGnsNItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoDzTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOTd4IN88US=> MlKyV2FPT0WU
human MN-60 cell Mmn6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2nDXWlvcGmkaYTpc44hd2ZiaIXtZY4hVU5vNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlM4PjJ|IN88US=> MY\TRW5ITVJ?
human NEC8 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NELSOWZKdmirYnn0bY9vKG:oIHj1cYFvKE6HQ{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlQ4ODd4IN88US=> MWPTRW5ITVJ?
human SJSA1 cells MlzEVJJwdGmoZYLheIlwdiCjc4PhfS=> M4TUcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1rTRVEh[2WubIOsJGlEPTB;ND61OUDPxE1? NXPjXoUxOTd|MEO0NlE>
human NCI-H2405 cell NIjLcIlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MojjTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK0NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01Njd2NEmzJO69VQ>? MVTTRW5ITVJ?
human YH-13 cell NXzybGRjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFHYUZhKdmirYnn0bY9vKG:oIHj1cYFvKFmKLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE44PTd5NjFOwG0> NGDFUW9USU6JRWK=
human SK-MEL-2 cell NV7XfIZpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn\VTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvODl4M{Kg{rxu MXjTRW5ITVJ?
human NB13 cell NELsRmNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2K0eWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|I4QDZizszN MYXTRW5ITVJ?
human GAMG cell MnnIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4\SbmlvcGmkaYTpc44hd2ZiaIXtZY4hT0GPRzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|cyPSEQvF2= MWPTRW5ITVJ?
human NCI-H1792 cell NULxeVlyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEjXR3FKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc6OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwNEe5OVQh|ryP Mle5V2FPT0WU
human KP-4 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVHnPIZ5UW6qaXLpeIlwdiCxZjDoeY1idiCNUD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QTh{MzFOwG0> NYD4fmZ6W0GQR1XS
human HGC-27 cell NYnNVoV4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFm5WWdKdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzNyN{eg{txO MVLTRW5ITVJ?
human GCT cell Ml;rS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGdEXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOEWxNlEh|ryP NWnycGd7W0GQR1XS
human PFSK-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYnJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN NX30UJQ1W0GQR1XS
human A172 cell MoTMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX3SOYFqUW6qaXLpeIlwdiCxZjDoeY1idiCDMUeyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46ODd7IN88US=> NIG2XVNUSU6JRWK=
human HO-1-N-1 cell M4jufmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml7pTY5pcWKrdHnvckBw\iCqdX3hckBJVy1zLV6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTV2MkWg{txO MkfPV2FPT0WU
human HN cell M1LtR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml7KTY5pcWKrdHnvckBw\iCqdX3hckBJViClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUewPVch|ryP MXXTRW5ITVJ?
human IST-MES1 cell M{e5Umdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3TMemlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLV3FV|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl5NUKxJO69VQ>? MWLTRW5ITVJ?
human TGW cell MnvOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXfJcohq[mm2aX;uJI9nKGi3bXHuJHRIXyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwMEW2PFIh|ryP NGKybnJUSU6JRWK=
human COR-L23 cell MkDOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV76dplOUW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFI{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi5|N{O1JO69VQ>? Mn\SV2FPT0WU
human D-566MG cell NV\5eXBKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGQuPTZ4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22Mlc5QDd|IN88US=> MVvTRW5ITVJ?
human SKG-IIIa cell NFrNb5JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;WTY5pcWKrdHnvckBw\iCqdX3hckBUU0dvSVnJZUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvQDV3N{Kg{txO MYLTRW5ITVJ?
human NCI-H446 cell MnXQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{jNZmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OFYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjBzOEGg{txO M{XCT3NCVkeHUh?=
human KYSE-270 cell MofLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVvJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOzh5MjFOwG0> M2fHWnNCVkeHUh?=
human CAL-39 cell M2G4dWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NG\HSohKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2zPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOzV{NDFOwG0> M2PwXnNCVkeHUh?=
human IST-SL1 cell MnzMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWjJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ{OzR7IN88US=> Moq0V2FPT0WU
human SF539 cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2nsfGlvcGmkaYTpc44hd2ZiaIXtZY4hW0Z3M{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlU2OTl5IN88US=> M3zyfXNCVkeHUh?=
human TE-1 cell M{jJXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVKwXW1QUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{45QTB7MTFOwG0> Mk\SV2FPT0WU
human NB10 cell NVL1cHdHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoLMTY5pcWKrdHnvckBw\iCqdX3hckBPSjFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz65PFc2QCEQvF2= MnTIV2FPT0WU
human 8505C cell NHrjXVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NU\KUnN7UW6qaXLpeIlwdiCxZjDoeY1idiB6NUC1R{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvQTl6MUeg{txO NYrGNZpDW0GQR1XS
human DB cell MoHTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVzJcohq[mm2aX;uJI9nKGi3bXHuJGRDKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5yMUGzNUDPxE1? MWjTRW5ITVJ?
human NCI-H28 cell M4Owbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIPweFZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjV7OEi0JO69VQ>? Ml[4V2FPT0WU
human LOXIMVI cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mo\1TY5pcWKrdHnvckBw\iCqdX3hckBNV1iLTW\JJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE43OzdyMTFOwG0> M{DzUHNCVkeHUh?=
human ME-180 cell Mlv4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnTOTY5pcWKrdHnvckBw\iCqdX3hckBOTS1zOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlgxOzl3IN88US=> NWGxOVFFW0GQR1XS
human ES3 cell M32zeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NULje3dpUW6qaXLpeIlwdiCxZjDoeY1idiCHU{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlEzPzl2IN88US=> MmH1V2FPT0WU
human SK-N-FI cell M2L4SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGDYSYNKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjRzNkm5JO69VQ>? NUfobGF3W0GQR1XS
human NBsusSR cell NG\kVGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF7lem5KdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5{M{G4JO69VQ>? MV3TRW5ITVJ?
human MCF7 cell MmnSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHS2R4lKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> M1fhbXNCVkeHUh?=
human A549 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX\SVZNNUW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB4IN88US=> NX21NYFpW0GQR1XS
human HuP-T4 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUf6bZJXUW6qaXLpeIlwdiCxZjDoeY1idiCKdWCtWFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC52MkexJO69VQ>? MXHTRW5ITVJ?
human HSC-2 cell MmDDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmTxTY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMFc5PDJizszN NFvjVGlUSU6JRWK=
human A253 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmS3TY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNlg{PCEQvF2= MkPRV2FPT0WU
human HCT-116 cell NGO1blVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFLHPJZKdmirYnn0bY9vKG:oIHj1cYFvKFCFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE4xODR|IN88US=> M33xeXNCVkeHUh?=
human HCT-116 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWnYT4RSUW6qaXLpeIlwdiCxZjDoeY1idiCKQ2StNVE3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTlwNk[4JO69VQ>? M1nvTXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID