Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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Cited by 10 Publications

5 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

    Cell cycle synchronization in M phase. Cells were treated with 6 µM RO-3306 for 20 h. After release from RO-3306 treatment, the cells were fixed at 0.5, 1, and 1.5 h after the release. α-tubulin (green), DNA (red). Scale bars, 20 µm.

    Int J Mol Sci, 2018, 19(12). Ro-3306 purchased from Selleck.

  • (A). Cell toxicity of CDK1 Inhibitor (RO‐3306) or CDK6 (Palbociclib HCl) on Huh7 cells. (B). Flow cytometry analysis of the cell cycle by CDK1 inhibitor (5 μm) or CDK6 inhibitor (2.5 μm) treatment.

    FEBS Lett, 2018, 592(11):1893-1904. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4PIT2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55M{C5OUDPxE1? NH3YZYdUSU6JRWK=
human H460a cells M2jPSXBzd2yrZnXyZZRqd25iYYPzZZk> MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEh2NkDhJINmdGy|LDDJR|UxRTFwMESg{txO NUi1O|I{OTd|MEO0NlE>
human MDA435 cells NUC2d|l6WHKxbHnm[ZJifGmxbjDhd5NigQ>? MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQUSzOUBk\WyuczygTWM2OD1zLkG0JO69VQ>? NX7wc5d[OTd|MEO0NlE>
human HCT116 cells M2XMenBzd2yrZnXyZZRqd25iYYPzZZk> NH7EW4VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3ME2xMlE1KM7:TR?= NYHPSXkyOTd|MEO0NlE>
human H4 cell MmTjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mlz0TY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUW4O|Uh|ryP MWXTRW5ITVJ?
human PA-1 cell NF;xV3RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{HTTmlvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlI3PTRizszN MoLHV2FPT0WU
human T98G cell MnX5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUDOdmg{UW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjhzMjFOwG0> MWDTRW5ITVJ?
human SW1710 cell NHzUSIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1zpUWlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43OTRyODFOwG0> M{K5bXNCVkeHUh?=
human U251 cell MoqxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkjETY5pcWKrdHnvckBw\iCqdX3hckBWOjVzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|c4OSEQvF2= MXjTRW5ITVJ?
human ALL-PO cell M1i1bmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmPRTY5pcWKrdHnvckBw\iCqdX3hckBCVExvUF:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVEvQTB6MUOg{txO NWfTZXJqW0GQR1XS
human RKO cells NH\q[Y5Rem:uaX\ldoF1cW:wIHHzd4F6 M3zPXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUlvPJINmdGy|LDDJR|UxRTJizszN MmTYNVc{ODN2MkG=
human SK-N-DZ cell NWmzXGU3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNU5vRGqgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlAyQDd4IN88US=> MVLTRW5ITVJ?
human A4-Fuk cell M2CwSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1;Gc2lvcGmkaYTpc44hd2ZiaIXtZY4hSTRvRoXrJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4xPTZyNDFOwG0> MlPrV2FPT0WU
human SW480 cells MnvuVJJwdGmoZYLheIlwdiCjc4PhfS=> MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOZNEiwJINmdGy|LDDJR|UxRTJwMjFOwG0> NXvOdllnOTd|MEO0NlE>
human 639-V cell Mom1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVfyPWVRUW6qaXLpeIlwdiCxZjDoeY1idiB4M{mtWkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOjd3M{eg{txO NULabGJ{W0GQR1XS
human Hs-578-T cell Ml3IS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHvGcIhKdmirYnn0bY9vKG:oIHj1cYFvKEi|LUW3PE1VKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5{N{e5PUDPxE1? MoPnV2FPT0WU
human UM-UC-3 cell NY\2TZlxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MorETY5pcWKrdHnvckBw\iCqdX3hckBWVS2XQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0zNjd|OUO2JO69VQ>? NF3jOYRUSU6JRWK=
human BPH-1 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXnRZ|lNUW6qaXLpeIlwdiCxZjDoeY1idiCEUFitNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvQTd2NUWg{txO MYnTRW5ITVJ?
human 5637 cell NVnlbJo4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUPZWVBEUW6qaXLpeIlwdiCxZjDoeY1idiB3NkO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4xQDV|MjFOwG0> NYjneHJnW0GQR1XS
human 8-MG-BA cell NFrlWWlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3\2[GlvcGmkaYTpc44hd2ZiaIXtZY4hQC2PRz3CRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oy5zNEC0PUDPxE1? NID5RnZUSU6JRWK=
human HCT116 cells MmLzR5l1d3SxeHnjxsBie3OjeR?= M4fNS|Uh\GG7cx?= MoDBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGGodHXyJFUh\GG7czDifUBOXFNiYYPzZZnwxIxiSVO1NF0{NjJizszN M{f6T|IzOzJ4MU[4
human LB1047-RCC cell NV7i[mxST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmL3TY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{WwNUDPxE1? NH;2W2dUSU6JRWK=
human KOSC-2 cell M1qxWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUfwXGs{UW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR2Nk[2NkDPxE1? MVTTRW5ITVJ?
human COR-L88 cell NH6xWJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{KyPWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVy4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDd3OEOg{txO MV;TRW5ITVJ?
human PSN1 cell M1HhZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHPpR5BKdmirYnn0bY9vKG:oIHj1cYFvKFCVTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlExQDB3IN88US=> MXHTRW5ITVJ?
human LXF-289 cell NX3j[2xlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmKxTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOTd4IN88US=> MoHXV2FPT0WU
human MN-60 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= MVXTRW5ITVJ?
human NEC8 cell NIjTOmlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHrJO|BKdmirYnn0bY9vKG:oIHj1cYFvKE6HQ{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlQ4ODd4IN88US=> MVTTRW5ITVJ?
human SJSA1 cells M{\PeHBzd2yrZnXyZZRqd25iYYPzZZk> MkDTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSmPBNUBk\WyuczygTWM2OD12LkW1JO69VQ>? M{LZTFE4OzB|NEKx
human NCI-H2405 cell MnHTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3LITWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyOFA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC55NES5N{DPxE1? NVexelJRW0GQR1XS
human YH-13 cell M2jzNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUfoTZAyUW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzV5N{[g{txO NGD2Z3lUSU6JRWK=
human SK-MEL-2 cell NVnpPJdsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIS4O|NKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS5yOU[zNkDPpG1? MULTRW5ITVJ?
human NB13 cell M{n6RWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIPDbpNKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> NV7LOnJuW0GQR1XS
human GAMG cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;jT4wxUW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{PzF3IN88US=> NGK1TXBUSU6JRWK=
human NCI-H1792 cell NWPDVXdYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYOwWG55UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE4QTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkS3PVU1KM7:TR?= MnnlV2FPT0WU
human KP-4 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV;mfXI6UW6qaXLpeIlwdiCxZjDoeY1idiCNUD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QTh{MzFOwG0> NH;YeGNUSU6JRWK=
human HGC-27 cell NF;Z[|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUTJcohq[mm2aX;uJI9nKGi3bXHuJGhISy1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuO|MxPzdizszN MkXqV2FPT0WU
human GCT cell NG\TeZVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1nKVWlvcGmkaYTpc44hd2ZiaIXtZY4hT0OWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT64OVEzOSEQvF2= MWDTRW5ITVJ?
human PFSK-1 cell NUDCU5J7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN MYXTRW5ITVJ?
human A172 cell M13zXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1HTZWlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVA4QSEQvF2= MXTTRW5ITVJ?
human HO-1-N-1 cell NYixT4h5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX\4[ppLUW6qaXLpeIlwdiCxZjDoeY1idiCKTz2xMW4uOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUW0NlUh|ryP NGPGdmhUSU6JRWK=
human HN cell NUDPcpBpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{DBc2lvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lkm3NFk4KM7:TR?= MmLTV2FPT0WU
human IST-MES1 cell M2[5PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWnJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2PRWOxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PzV{MTFOwG0> MknXV2FPT0WU
human TGW cell M3PPW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1XlUmlvcGmkaYTpc44hd2ZiaIXtZY4hXEeZIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6wOVY5OiEQvF2= M1jhSXNCVkeHUh?=
human COR-L23 cell NFf6WYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXfmPHBvUW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFI{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi5|N{O1JO69VQ>? NHvrOldUSU6JRWK=
human D-566MG cell NGL2fW1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnPHTY5pcWKrdHnvckBw\iCqdX3hckBFNTV4Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok44QDh5MzFOwG0> MUfTRW5ITVJ?
human SKG-IIIa cell M4DU[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NU[3Z2hPUW6qaXLpeIlwdiCxZjDoeY1idiCVS1etTWlK[SClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwOEW1O|Ih|ryP NVi4fWhNW0GQR1XS
human NCI-H446 cell M4PUO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNES2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOThzIN88US=> M{f2d3NCVkeHUh?=
human KYSE-270 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVnqSIx2UW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEO4O|Ih|ryP NHqxT5lUSU6JRWK=
human CAL-39 cell NIfIeZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmrwTY5pcWKrdHnvckBw\iCqdX3hckBESUxvM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlM2OjRizszN MmDEV2FPT0WU
human IST-SL1 cell M2rxe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ{OzR7IN88US=> NIfE[IlUSU6JRWK=
human SF539 cell NFHrZVNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEL2OWJKdmirYnn0bY9vKG:oIHj1cYFvKFOINUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{42PTF7NzFOwG0> MkPoV2FPT0WU
human TE-1 cell M{LXdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGHSRnFKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlg6ODlzIN88US=> NIK0fGVUSU6JRWK=
human NB10 cell Ml;VS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHnTe4FKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlk5PzV6IN88US=> M{nnW3NCVkeHUh?=
human 8505C cell NVPGPJNwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWXQUmYyUW6qaXLpeIlwdiCxZjDoeY1idiB6NUC1R{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvQTl6MUeg{txO M{PVWXNCVkeHUh?=
human DB cell NWXTUoR7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHLFe2tKdmirYnn0bY9vKG:oIHj1cYFvKESEIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6wNVE{OSEQvF2= NH3rTJVUSU6JRWK=
human NCI-H28 cell NIHCXpNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnSTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42QTh6NDFOwG0> MmLvV2FPT0WU
human LOXIMVI cell NGLoUlhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXnJcohq[mm2aX;uJI9nKGi3bXHuJGxQYEmPVlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY{PzBzIN88US=> M1;oXXNCVkeHUh?=
human ME-180 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1O2cWlvcGmkaYTpc44hd2ZiaIXtZY4hVUVvMUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE45ODN7NTFOwG0> M1HKVnNCVkeHUh?=
human ES3 cell NGPwOGFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTlwMUK3PVQh|ryP NF;COo5USU6JRWK=
human SK-N-FI cell M4HYNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4rQbWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3GTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPDF4OUmg{txO NHm5SIZUSU6JRWK=
human NBsusSR cell NFHYeYFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MljHTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOjNzODFOwG0> MVHTRW5ITVJ?
human MCF7 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVLJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6xPFQ6KM7:TR?= MmLSV2FPT0WU
human A549 cell NVLEXIM5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWHSNXE5UW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB4IN88US=> MmD5V2FPT0WU
human HuP-T4 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlvZTY5pcWKrdHnvckBw\iCqdX3hckBJfVBvVESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE41OjdzIN88US=> NX\j[ohNW0GQR1XS
human HSC-2 cell NHzMSnBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MojUTY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMFc5PDJizszN Ml7DV2FPT0WU
human A253 cell NULIT3FPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mo\vTY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNlg{PCEQvF2= NFKzNHZUSU6JRWK=
human HCT-116 cell NGH0RZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1TQcWlvcGmkaYTpc44hd2ZiaIXtZY4hWENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlAxPDNizszN M4XlO3NCVkeHUh?=
human HCT-116 cell NWDUR2tvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPU43PjhizszN MmfvV2FPT0WU

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID