Ro-3306

For research use only.

Catalog No.S7747

44 publications

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis.

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Selleck's Ro-3306 has been cited by 44 publications

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NFfGW|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2e5T2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55M{C5OUDPxE1? MV;TRW5ITVJ?
human H460a cells M32xd3Bzd2yrZnXyZZRqd25iYYPzZZk> NYH4XVNFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYx[SClZXzsd{whUUN3ME2xMlA1KM7:TR?= NUW4NG16OTd|MEO0NlE>
human MDA435 cells NFu5XJRRem:uaX\ldoF1cW:wIHHzd4F6 MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQUSzOUBk\WyuczygTWM2OD1zLkG0JO69VQ>? MU[xO|MxOzR{MR?=
human HCT116 cells NITjeoVRem:uaX\ldoF1cW:wIHHzd4F6 NGLFdnhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3ME2xMlE1KM7:TR?= NHHJXZMyPzNyM{SyNS=>
human H4 cell NYHG[4tnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUnhdFd2UW6qaXLpeIlwdiCxZjDoeY1idiCKNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNVU5PzVizszN NUPISmxuW0GQR1XS
human PA-1 cell NF7iXphIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4GxXWlvcGmkaYTpc44hd2ZiaIXtZY4hWEFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlI3PTRizszN NX\PVFVlW0GQR1XS
human T98G cell NUTj[mNmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoDWTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zOlgyOiEQvF2= MlnWV2FPT0WU
human SW1710 cell MofhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXXJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlE1ODhizszN MU\TRW5ITVJ?
human U251 cell M4rXOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV7tSWoxUW6qaXLpeIlwdiCxZjDoeY1idiCXMkWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43Ozd5MTFOwG0> MlXsV2FPT0WU
human ALL-PO cell NFLjWmhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2nDS2lvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZnwxIxiSVO1NF0yNjlyOEGzJO69VQ>? MorzV2FPT0WU
human RKO cells M3XZW3Bzd2yrZnXyZZRqd25iYYPzZZk> MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyxiSVO1NF0zKM7:TR?= NGnYcYcyPzNyM{SyNS=>
human SK-N-DZ cell Ml\oS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFfJW2RKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjBzOEe2JO69VQ>? MVfTRW5ITVJ?
human A4-Fuk cell NEmzPHFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGE1NU[3azDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNFU3ODRizszN NYnBdXF7W0GQR1XS
human SW480 cells MW\Qdo9tcW[ncnH0bY9vKGG|c3H5 MlryRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczygTWM2OD1{LkKg{txO NWfmRmJqOTd|MEO0NlE>
human 639-V cell NFfCOZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3HIRWlvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlI4PTN5IN88US=> NUTuVWhTW0GQR1XS
human Hs-578-T cell NFX3ZnlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYXkOopKUW6qaXLpeIlwdiCxZjDoeY1idiCKcz21O|guXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMke3PVkh|ryP NVfUfXo1W0GQR1XS
human UM-UC-3 cell Mne0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJHVONVWFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4Phfg+9lCCLQ{WwQVIvPzN7M{[g{txO MUDTRW5ITVJ?
human BPH-1 cell NF7BfFVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXHJcohq[mm2aX;uJI9nKGi3bXHuJGJRUC1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj65O|Q2PSEQvF2= NYr5eXNLW0GQR1XS
human 5637 cell NGHqWpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX2zOIw5UW6qaXLpeIlwdiCxZjDoeY1idiB3NkO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4xQDV|MjFOwG0> NHfSd5BUSU6JRWK=
human 8-MG-BA cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW[xXFV{UW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1|LkG0NFQ6KM7:TR?= NXzOfWxkW0GQR1XS
human HCT116 cells MWLDfZRwfG:6aXRCpIF{e2G7 MlWzOUBl[Xm| NIDlU29EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5Nige,:jDDJR|UxRTNwMjFOwG0> MVGyNlMzPjF4OB?=
human LB1047-RCC cell M4jFO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXPOOI5PUW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yN|UxOSEQvF2= MWLTRW5ITVJ?
human KOSC-2 cell M2frOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoXJTY5pcWKrdHnvckBw\iCqdX3hckBMV1OFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlQ1PjZ4MjFOwG0> MkT5V2FPT0WU
human COR-L88 cell Ml3VS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVLPS|R{UW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFg5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy52N{W4N{DPxE1? NGTQbZVUSU6JRWK=
human PSN1 cell MoPzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH7sbmlKdmirYnn0bY9vKG:oIHj1cYFvKFCVTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlExQDB3IN88US=> MWHTRW5ITVJ?
human LXF-289 cell NHnFRWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M33lV2lvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvOjF5NjFOwG0> NX7CdIJMW0GQR1XS
human MN-60 cell Ml7uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWnJcohq[mm2aX;uJI9nKGi3bXHuJG1PNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6zO|YzOyEQvF2= NGL3bIhUSU6JRWK=
human NEC8 cell M2H6Smdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFy4dppKdmirYnn0bY9vKG:oIHj1cYFvKE6HQ{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlQ4ODd4IN88US=> NEn0WFZUSU6JRWK=
human SJSA1 cells NE\aNWpRem:uaX\ldoF1cW:wIHHzd4F6 M{TjemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1rTRVEh[2WubIOsJGlEPTB;ND61OUDPxE1? NFn5cW8yPzNyM{SyNS=>
human NCI-H2405 cell NUSxUFAxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{PBO2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyOFA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC55NES5N{DPxE1? NGPve5NUSU6JRWK=
human YH-13 cell NYDCXXpGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXHEfmJ3UW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzV5N{[g{txO NVSwUXM{W0GQR1XS
human SK-MEL-2 cell NW\POlNJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoD4TY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvODl4M{Kg{rxu MXXTRW5ITVJ?
human NB13 cell NITWfY1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHvVTpFKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> NXfQfHh{W0GQR1XS
human GAMG cell NIDJd|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGdCVUdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkO3NVUh|ryP MVLTRW5ITVJ?
human NCI-H1792 cell M{fHcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXTJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPDd7NUSg{txO MWPTRW5ITVJ?
human KP-4 cell NF7hbYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4HpeWlvcGmkaYTpc44hd2ZiaIXtZY4hU1BvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuOlk5OjNizszN MlHPV2FPT0WU
human HGC-27 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml\6TY5pcWKrdHnvckBw\iCqdX3hckBJT0NvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlc{ODd5IN88US=> NFrDUnFUSU6JRWK=
human GCT cell MmfNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWmydmlwUW6qaXLpeIlwdiCxZjDoeY1idiCJQ2SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21Mlg2OTJzIN88US=> MXnTRW5ITVJ?
human PFSK-1 cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXTNdXh6UW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njh4MU[4JO69VQ>? NWH1fmNsW0GQR1XS
human A172 cell M1jub2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HmNGlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVA4QSEQvF2= NY\BdW14W0GQR1XS
human HO-1-N-1 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1y5ZmlvcGmkaYTpc44hd2ZiaIXtZY4hUE9vMT3OMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl3NEK1JO69VQ>? MXXTRW5ITVJ?
human HN cell MkXHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MliwTY5pcWKrdHnvckBw\iCqdX3hckBJViClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUewPVch|ryP M2fkcHNCVkeHUh?=
human IST-MES1 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1rRXmlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLV3FV|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl5NUKxJO69VQ>? NUC0eFRzW0GQR1XS
human TGW cell NEXiVWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXrkdJFwUW6qaXLpeIlwdiCxZjDoeY1idiCWR2egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2Pjh{IN88US=> M4\xe3NCVkeHUh?=
human COR-L23 cell NFK5Z2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLIW4xyUW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFI{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi5|N{O1JO69VQ>? NGHuRWhUSU6JRWK=
human D-566MG cell NEjFVWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUXZbopIUW6qaXLpeIlwdiCxZjDoeY1idiCGLUW2Om1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi55OEi3N{DPxE1? NX3JflRtW0GQR1XS
human SKG-IIIa cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\6V4NKdmirYnn0bY9vKG:oIHj1cYFvKFONRz3JTWliKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi56NUW3NkDPxE1? MnHPV2FPT0WU
human NCI-H446 cell NYnuS2FKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnjxTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSES0OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODF6MTFOwG0> MWnTRW5ITVJ?
human KYSE-270 cell NGTlNIZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVHlRYNWUW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEO4O|Ih|ryP NH23fFlUSU6JRWK=
human CAL-39 cell NY\oU5hIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3f3XGlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4{PTJ2IN88US=> Mn35V2FPT0WU
human IST-SL1 cell NIDuZWpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH\xfm9KdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjR|M{S5JO69VQ>? MkTjV2FPT0WU
human SF539 cell NYLT[4ZMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn\RTY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuOVUyQTdizszN NY\uOIVmW0GQR1XS
human TE-1 cell MlriS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlXyTY5pcWKrdHnvckBw\iCqdX3hckBVTS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz64PVA6OSEQvF2= NYLRVZE6W0GQR1XS
human NB10 cell M3W4bWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWfJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15Lkm4O|U5KM7:TR?= NI\lSXpUSU6JRWK=
human 8505C cell M2TSWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEOxT4lKdmirYnn0bY9vKG:oIHj1cYFvKDh3MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QThzNzFOwG0> MYXTRW5ITVJ?
human DB cell NFvIfYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGnLNIhKdmirYnn0bY9vKG:oIHj1cYFvKESEIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6wNVE{OSEQvF2= MUXTRW5ITVJ?
human NCI-H28 cell NVHh[ldzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHPlNIRKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjV7OEi0JO69VQ>? NG\yflJUSU6JRWK=
human LOXIMVI cell NVKyPFJKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3;6VmlvcGmkaYTpc44hd2ZiaIXtZY4hVE:[SV3WTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPjN5MEGg{txO MmrrV2FPT0WU
human ME-180 cell M1juOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkK4TY5pcWKrdHnvckBw\iCqdX3hckBOTS1zOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlgxOzl3IN88US=> M2nKSnNCVkeHUh?=
human ES3 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEfTeYlKdmirYnn0bY9vKG:oIHj1cYFvKEWVMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuNVI4QTRizszN M4WxT3NCVkeHUh?=
human SK-N-FI cell NGPrVI9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNU5vRlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlQyPjl7IN88US=> MlrxV2FPT0WU
human NBsusSR cell NXvRZld6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUXQbVEzUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwMkOxPEDPxE1? M4PNOnNCVkeHUh?=
human MCF7 cell M1XQVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{HZUGlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlE5PDlizszN NUL6XnZIW0GQR1XS
human A549 cell MmPvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfSOGdKdmirYnn0bY9vKG:oIHj1cYFvKEF3NEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4zODZizszN M2LBS3NCVkeHUh?=
human HuP-T4 cell M3LV[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYfJcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlQzPzFizszN M{TUO3NCVkeHUh?=
human HSC-2 cell M1PGWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGhUSy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGsO|g1OiEQvF2= NHnXS5hUSU6JRWK=
human A253 cell M1H4bGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnvITY5pcWKrdHnvckBw\iCqdX3hckBCOjV|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNlg{PCEQvF2= MnfRV2FPT0WU
human HCT-116 cell Mmn1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1vWTWlvcGmkaYTpc44hd2ZiaIXtZY4hWENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlAxPDNizszN MVTTRW5ITVJ?
human HCT-116 cell Mmr2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV6wfJZoUW6qaXLpeIlwdiCxZjDoeY1idiCKQ2StNVE3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTlwNk[4JO69VQ>? Ml2xV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 for 24 h in indicated dose dependent manner, (F) 10 uM RO-3306 for 12 h up to 48 h. The expression level of the indicated protein and the phosphorylated form of H2A.x and p38 were examined via Western blot analyses.

27385216
Immunofluorescence
PARP1 / Plk1 ; 

PubMed: 28069876     


Representative images of IF staining for PARP1 and Plk1 in 22RV1 cells treated with 0.3 mM mimosine for 20 h, 4 mM hydroxyurea for 24 h, 10 µM RO-3306 for 7 h or 200 ng/ml nocodazole (Noc) for 12 h.

28069876
Growth inhibition assay
Cell viability; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 in indicated time- and dose-dependent manner. Cell viability were measured via crystal violet assay. Results are the means ± S.E. n = 3. 

27385216

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A
Smiles O=C1N=C(NCC2=CC=CS2)S\C1=C/C3=CC=C4N=CC=CC4=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID