Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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3 Customer Reviews

  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

    A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2VTmWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPzNyOUWg{txO NXLXOppiW0GQR1XS
human H460a cells NWj1RpVYWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{j1O2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSES2NIEh[2WubIOsJGlEPTB;MT6wOEDPxE1? MW[xO|MxOzR{MR?=
human MDA435 cells NHK5WY1Rem:uaX\ldoF1cW:wIHHzd4F6 MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQUSzOUBk\WyuczygTWM2OD1zLkG0JO69VQ>? NHXKOnYyPzNyM{SyNS=>
human HCT116 cells M1vYSHBzd2yrZnXyZZRqd25iYYPzZZk> MlHsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOsJGlEPTB;MT6xOEDPxE1? MmWwNVc{ODN2MkG=
human H4 cell NWG5OGdNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5zNUi3OUDPxE1? M33tXHNCVkeHUh?=
human PA-1 cell MkjaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGTL[llKdmirYnn0bY9vKG:oIHj1cYFvKFCDLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIzPjV2IN88US=> NYTFWFZiW0GQR1XS
human T98G cell M33oWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlfKTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zOlgyOiEQvF2= M1;tWHNCVkeHUh?=
human SW1710 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUPJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlE1ODhizszN MX3TRW5ITVJ?
human U251 cell MknES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF3mRWtKdmirYnn0bY9vKG:oIHj1cYFvKFV{NUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY{PzdzIN88US=> NUTwPFQxW0GQR1XS
human ALL-PO cell NIG4XFVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWrJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H589yNKEmFNUC9NU46ODhzMzFOwG0> M3X2dXNCVkeHUh?=
human RKO cells MV3Qdo9tcW[ncnH0bY9vKGG|c3H5 MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyxiSVO1NF0zKM7:TR?= M2\S[lE4OzB|NEKx
human SK-N-DZ cell NVn3c45RT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYqxNXBxUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWRbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5yMUi3OkDPxE1? NGPqeI1USU6JRWK=
human A4-Fuk cell NInt[GtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYnO[ndJUW6qaXLpeIlwdiCxZjDoeY1idiCDND3GeYsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB3NkC0JO69VQ>? M3HoRXNCVkeHUh?=
human SW480 cells NX24eFBHWHKxbHnm[ZJifGmxbjDhd5NigQ>? Mor0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczygTWM2OD1{LkKg{txO NWnRU5RGOTd|MEO0NlE>
human 639-V cell MnPkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk\JTY5pcWKrdHnvckBw\iCqdX3hckA3OzlvVjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNlc2OzdizszN M2P6[3NCVkeHUh?=
human Hs-578-T cell NYXRWpo4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX3CUnhjUW6qaXLpeIlwdiCxZjDoeY1idiCKcz21O|guXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMke3PVkh|ryP NUCz[lN4W0GQR1XS
human UM-UC-3 cell NFTqNYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIX2dXZKdmirYnn0bY9vKG:oIHj1cYFvKFWPLWXDMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTJwN{O5N|Yh|ryP MmPyV2FPT0WU
human BPH-1 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4[1V2lvcGmkaYTpc44hd2ZiaIXtZY4hSlCKLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlk4PDV3IN88US=> MomwV2FPT0WU
human 5637 cell NWGyfm1YT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHXCN5VKdmirYnn0bY9vKG:oIHj1cYFvKDV4M{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlA5PTN{IN88US=> MWXTRW5ITVJ?
human 8-MG-BA cell NUfTXmFPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3rzOGlvcGmkaYTpc44hd2ZiaIXtZY4hQC2PRz3CRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oy5zNEC0PUDPxE1? M2fQ[HNCVkeHUh?=
human HCT116 cells Mk\ZR5l1d3SxeHnjxsBie3OjeR?= MVu1JIRigXN? NU\GdZlPS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDViZHH5d{BjgSCPVGOgZZN{[XoxvJygTWM2OD1|LkKg{txO M{DaSlIzOzJ4MU[4
human LB1047-RCC cell NGrmNohIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJGxDOTB2Nz3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjJ|NUCxJO69VQ>? M3;WUnNCVkeHUh?=
human KOSC-2 cell NHm4WZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3niUWlvcGmkaYTpc44hd2ZiaIXtZY4hU0:VQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41PDZ4NkKg{txO NVLMTGJTW0GQR1XS
human COR-L88 cell NX3D[IxqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml3FTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEi4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41PzV6MzFOwG0> MkDnV2FPT0WU
human PSN1 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M370XWlvcGmkaYTpc44hd2ZiaIXtZY4hWFOQMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNVA5ODVizszN NX3ZO5pvW0GQR1XS
human LXF-289 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlIyPzZizszN MVPTRW5ITVJ?
human MN-60 cell NEDUe5lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkXuTY5pcWKrdHnvckBw\iCqdX3hckBOVi14MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuN|c3OjNizszN MnT5V2FPT0WU
human NEC8 cell M3nLXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkL4TY5pcWKrdHnvckBw\iCqdX3hckBPTUN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND60O|A4PiEQvF2= NIfKNXVUSU6JRWK=
human SJSA1 cells Mnj1VJJwdGmoZYLheIlwdiCjc4PhfS=> NInXd4ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPKV2EyKGOnbHzzMEBKSzVyPUSuOVUh|ryP NFO2bJIyPzNyM{SyNS=>
human NCI-H2405 cell M1yyemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXTZSGJIUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI1ODViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lke0OFk{KM7:TR?= M{TzPHNCVkeHUh?=
human YH-13 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWHiXWV1UW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzV5N{[g{txO MVjTRW5ITVJ?
human SK-MEL-2 cell M{PwNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnqyTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvODl4M{Kg{rxu MWDTRW5ITVJ?
human NB13 cell M4jMNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFfUPZRKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> NEnDU3pUSU6JRWK=
human GAMG cell NUjyWVJVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEjhbHJKdmirYnn0bY9vKG:oIHj1cYFvKEeDTVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM4OTVizszN NYPaVnd3W0GQR1XS
human NCI-H1792 cell NUHDbVBWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWjJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPDd7NUSg{txO MoHqV2FPT0WU
human KP-4 cell M1\Mcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEHKfplKdmirYnn0bY9vKG:oIHj1cYFvKEuSLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlY6QDJ|IN88US=> NXfjd|hOW0GQR1XS
human HGC-27 cell Ml;ZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUHJcohq[mm2aX;uJI9nKGi3bXHuJGhISy1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuO|MxPzdizszN NHLmO2xUSU6JRWK=
human GCT cell M3\0VGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2Lre2lvcGmkaYTpc44hd2ZiaIXtZY4hT0OWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT64OVEzOSEQvF2= NY\VbZZMW0GQR1XS
human PFSK-1 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF62elBKdmirYnn0bY9vKG:oIHj1cYFvKFCIU1utNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQDZzNkig{txO NETTSo5USU6JRWK=
human A172 cell NEjOR4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXu2UJlkUW6qaXLpeIlwdiCxZjDoeY1idiCDMUeyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46ODd7IN88US=> MlnSV2FPT0WU
human HO-1-N-1 cell NUjIXXRnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKGi3bXHuJGhQNTFvTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PTR{NTFOwG0> Mm\4V2FPT0WU
human HN cell NXrkOFVKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYHJcohq[mm2aX;uJI9nKGi3bXHuJGhPKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{C5O{DPxE1? NV;ISY96W0GQR1XS
human IST-MES1 cell MXnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUHldo5NUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StUWVUOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUe1NlEh|ryP NV\Q[nhmW0GQR1XS
human TGW cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWXJcohq[mm2aX;uJI9nKGi3bXHuJHRIXyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwMEW2PFIh|ryP NV;MZox3W0GQR1XS
human COR-L23 cell NVrWflljT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MofYTY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEKzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{PzN3IN88US=> NGTId4lUSU6JRWK=
human D-566MG cell NIHUUY5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF\CVJBKdmirYnn0bY9vKG:oIHj1cYFvKERvNU[2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njd6OEezJO69VQ>? NIfScmRUSU6JRWK=
human SKG-IIIa cell MmDlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJHNMTy2LSVnhJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok45PTV5MjFOwG0> MYLTRW5ITVJ?
human NCI-H446 cell NUXJXXFmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfLU4tKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOFQ3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yMUixJO69VQ>? NHThVWxUSU6JRWK=
human KYSE-270 cell NEOz[ZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF\HeFNKdmirYnn0bY9vKG:oIHj1cYFvKEu\U1WtNlcxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yM{i3NkDPxE1? M1jvcnNCVkeHUh?=
human CAL-39 cell MkHsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfr[4VKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2zPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOzV{NDFOwG0> MnrrV2FPT0WU
human IST-SL1 cell M3XxSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NU\3SIVwUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py52M{O0PUDPxE1? NWP4PJp{W0GQR1XS
human SF539 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYjJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz61OVE6PyEQvF2= NUHsTYlVW0GQR1XS
human TE-1 cell NVuxcJRrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml;JTY5pcWKrdHnvckBw\iCqdX3hckBVTS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz64PVA6OSEQvF2= M1HlW3NCVkeHUh?=
human NB10 cell Mnz0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmPITY5pcWKrdHnvckBw\iCqdX3hckBPSjFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz65PFc2QCEQvF2= M3rZenNCVkeHUh?=
human 8505C cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHTRb3JKdmirYnn0bY9vKG:oIHj1cYFvKDh3MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{46QThzNzFOwG0> MUfTRW5ITVJ?
human DB cell NEnMV4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2XocmlvcGmkaYTpc44hd2ZiaIXtZY4hTEJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkCxNVMyKM7:TR?= MXPTRW5ITVJ?
human NCI-H28 cell M2PIW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYnJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlU6QDh2IN88US=> NGXSe5lUSU6JRWK=
human LOXIMVI cell NY\EeGt[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHrk[XdKdmirYnn0bY9vKG:oIHj1cYFvKEyRWFnNWmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjZ|N{CxJO69VQ>? NWHmNlUxW0GQR1XS
human ME-180 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX\CUIxNUW6qaXLpeIlwdiCxZjDoeY1idiCPRT2xPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjhyM{m1JO69VQ>? NYDTSXpTW0GQR1XS
human ES3 cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmnjTY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkGyO|k1KM7:TR?= M4Xuc3NCVkeHUh?=
human SK-N-FI cell NVfaPHJvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNU5vRlmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlQyPjl7IN88US=> M13PZnNCVkeHUh?=
human NBsusSR cell NGfRW4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4DIfmlvcGmkaYTpc44hd2ZiaIXtZY4hVkK|dYPTVkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjJ|MUig{txO MoflV2FPT0WU
human MCF7 cell NVe1b29oT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF7aZXFKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> MXjTRW5ITVJ?
human A549 cell M1TDU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUfJcohq[mm2aX;uJI9nKGi3bXHuJGE2PDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yNFYh|ryP Ml\KV2FPT0WU
human HuP-T4 cell NWjveFhYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVf2TmlMUW6qaXLpeIlwdiCxZjDoeY1idiCKdWCtWFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC52MkexJO69VQ>? M{nlOnNCVkeHUh?=
human HSC-2 cell NEi2WYtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF;6cJRKdmirYnn0bY9vKG:oIHj1cYFvKEiVQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEtPzh2MjFOwG0> Mn;1V2FPT0WU
human A253 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{TU[WlvcGmkaYTpc44hd2ZiaIXtZY4hSTJ3MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG1MlI5OzRizszN Mo\HV2FPT0WU
human HCT-116 cell M2T6bWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkLmTY5pcWKrdHnvckBw\iCqdX3hckBRSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUiuNFA1OyEQvF2= MnvJV2FPT0WU
human HCT-116 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIrEPGFKdmirYnn0bY9vKG:oIHj1cYFvKEiFVD2xNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQS54Nkig{txO MWLTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID