Ro-3306

For research use only.

Catalog No.S7747

57 publications

Ro-3306 Chemical Structure

CAS No. 872573-93-8

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis.

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Selleck's Ro-3306 has been cited by 57 publications

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHe2UHRKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwN{OwPVUh|ryP MnuwV2FPT0WU
human H460a cells M4T6Z3Bzd2yrZnXyZZRqd25iYYPzZZk> Ml22RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKNE[wZUBk\WyuczygTWM2OD1zLkC0JO69VQ>? NEmzWlAyPzNyM{SyNS=>
human MDA435 cells NWewZmhRWHKxbHnm[ZJifGmxbjDhd5NigQ>? NIP5W2tCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERVQ{PSClZXzsd{whUUN3ME2xMlE1KM7:TR?= MU[xO|MxOzR{MR?=
human HCT116 cells MV3Qdo9tcW[ncnH0bY9vKGG|c3H5 NHXxPWdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3ME2xMlE1KM7:TR?= MlzqNVc{ODN2MkG=
human H4 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoXxTY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUW4O|Uh|ryP NXTsT2RQW0GQR1XS
human PA-1 cell MmSzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnfzTY5pcWKrdHnvckBw\iCqdX3hckBRSS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yNlY2PCEQvF2= NEHYSYJUSU6JRWK=
human T98G cell NVe4XVZUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFTxc5JKdmirYnn0bY9vKG:oIHj1cYFvKFR7OFegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlM3QDF{IN88US=> NYf6OopHW0GQR1XS
human SW1710 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2i1NmlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43OTRyODFOwG0> NY\Hd3I6W0GQR1XS
human U251 cell M1LMfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUXJcohq[mm2aX;uJI9nKGi3bXHuJHUzPTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLk[zO|cyKM7:TR?= MVPTRW5ITVJ?
human ALL-PO cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGHFZmJKdmirYnn0bY9vKG:oIHj1cYFvKEGOTD3QU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:OS57MEixN{DPxE1? M2LjdXNCVkeHUh?=
human RKO cells M4rJWXBzd2yrZnXyZZRqd25iYYPzZZk> M1m3cGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUlvPJINmdGy|LDDJR|UxRTJizszN M2GwRVE4OzB|NEKx
human SK-N-DZ cell MmfLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NED2dGpKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tSHoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjBzOEe2JO69VQ>? MlrVV2FPT0WU
human A4-Fuk cell NWrPVZk1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXnBUpBxUW6qaXLpeIlwdiCxZjDoeY1idiCDND3GeYsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB3NkC0JO69VQ>? MYTTRW5ITVJ?
human SW480 cells NHnNWIFRem:uaX\ldoF1cW:wIHHzd4F6 Ml[4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{S4NEBk\WyuczygTWM2OD1{LkKg{txO MnXONVc{ODN2MkG=
human 639-V cell M{nCWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{juS2lvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlI4PTN5IN88US=> M1fZbnNCVkeHUh?=
human Hs-578-T cell M2jWTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVridIN{UW6qaXLpeIlwdiCxZjDoeY1idiCKcz21O|guXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMke3PVkh|ryP MULTRW5ITVJ?
human UM-UC-3 cell NIG3O3hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2\0[mlvcGmkaYTpc44hd2ZiaIXtZY4hXU1vVVOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F697zOIFnDOVA:Oi55M{mzOkDPxE1? NVL4dllCW0GQR1XS
human BPH-1 cell MmPyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXzJcohq[mm2aX;uJI9nKGi3bXHuJGJRUC1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj65O|Q2PSEQvF2= NEDBWHJUSU6JRWK=
human 5637 cell NY\PS3J3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnPWTY5pcWKrdHnvckBw\iCqdX3hckA2PjN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6wPFU{OiEQvF2= NYGzZmx6W0GQR1XS
human 8-MG-BA cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NI\SRmNKdmirYnn0bY9vKG:oIHj1cYFvKDhvTVetRmEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTNwMUSwOFkh|ryP M2jBNXNCVkeHUh?=
human HCT116 cells MWnDfZRwfG:6aXRCpIF{e2G7 MVu1JIRigXN? NGWxZ4pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KE2WUzDhd5Nige,:jDDJR|UxRTNwMjFOwG0> NHPzUVQzOjN{NkG2PC=>
human LB1047-RCC cell M1XteWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX\QTHl4UW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6yN|UxOSEQvF2= NFjMXZdUSU6JRWK=
human KOSC-2 cell NX25OlBuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGnoVphKdmirYnn0bY9vKG:oIHj1cYFvKEuRU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvPDR4Nk[yJO69VQ>? NUHxc2xGW0GQR1XS
human COR-L88 cell NW\iW5pNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUTsb281UW6qaXLpeIlwdiCxZjDoeY1idiCFT2KtUFg5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy52N{W4N{DPxE1? M2n6d3NCVkeHUh?=
human PSN1 cell NV22N2ZYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFjSbVNKdmirYnn0bY9vKG:oIHj1cYFvKFCVTkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlExQDB3IN88US=> M1vNbHNCVkeHUh?=
human LXF-289 cell M3e3c2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkXjTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOTd4IN88US=> NIfHR5pUSU6JRWK=
human MN-60 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIT0TZJKdmirYnn0bY9vKG:oIHj1cYFvKE2QLU[wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4{PzZ{MzFOwG0> NHrwS2hUSU6JRWK=
human NEC8 cell M37iemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYjaO5FTUW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE41PzB5NjFOwG0> M3vFR3NCVkeHUh?=
human SJSA1 cells NHHVUVdRem:uaX\ldoF1cW:wIHHzd4F6 M{jjcWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1rTRVEh[2WubIOsJGlEPTB;ND61OUDPxE1? NIDCN4syPzNyM{SyNS=>
human NCI-H2405 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWHrXmFXUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI1ODViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lke0OFk{KM7:TR?= NX7RR|BqW0GQR1XS
human YH-13 cell NF25Z3NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND63OVc4PiEQvF2= M2S1XHNCVkeHUh?=
human SK-MEL-2 cell MonCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVXJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4xQTZ|MjFOoI0> NFPjUmtUSU6JRWK=
human NB13 cell NVvUWldlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGHESXFKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> NXHaeWM6W0GQR1XS
human GAMG cell NVnwTHQ3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfvTYpKdmirYnn0bY9vKG:oIHj1cYFvKEeDTVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM4OTVizszN NXvGUI8{W0GQR1XS
human NCI-H1792 cell NEDYZ2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWrvWGdFUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE4QTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkS3PVU1KM7:TR?= MVHTRW5ITVJ?
human KP-4 cell NFHMb3VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkW5TY5pcWKrdHnvckBw\iCqdX3hckBMWC12IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT62PVgzOyEQvF2= NIr1dZlUSU6JRWK=
human HGC-27 cell MnzyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2f4Z2lvcGmkaYTpc44hd2ZiaIXtZY4hUEeFLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44OzB5NzFOwG0> MUDTRW5ITVJ?
human GCT cell MmKyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVLJcohq[mm2aX;uJI9nKGi3bXHuJGdEXCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOEWxNlEh|ryP MoLtV2FPT0WU
human PFSK-1 cell NF;iWnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXnJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN NULOSWdOW0GQR1XS
human A172 cell NFHjUVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mlj5TY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT65NFc6KM7:TR?= NVzBWWt2W0GQR1XS
human HO-1-N-1 cell MkDOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWXJcohq[mm2aX;uJI9nKGi3bXHuJGhQNTFvTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PTR{NTFOwG0> MWXTRW5ITVJ?
human HN cell MmTvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3nNZ2lvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lkm3NFk4KM7:TR?= MYHTRW5ITVJ?
human IST-MES1 cell NGPBPWNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGjVWYtKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3NSXMyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{WyNUDPxE1? M2jzNnNCVkeHUh?=
human TGW cell NE\PSI9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4Dxe2lvcGmkaYTpc44hd2ZiaIXtZY4hXEeZIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6wOVY5OiEQvF2= MnfEV2FPT0WU
human COR-L23 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWDJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlM4OzVizszN Mk\aV2FPT0WU
human D-566MG cell Mmr4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmnSTY5pcWKrdHnvckBw\iCqdX3hckBFNTV4Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok44QDh5MzFOwG0> MVzTRW5ITVJ?
human SKG-IIIa cell NFXpSJBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXHUOGJFUW6qaXLpeIlwdiCxZjDoeY1idiCVS1etTWlK[SClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwOEW1O|Ih|ryP MYjTRW5ITVJ?
human NCI-H446 cell NIPJNJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3PvOGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OFYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjBzOEGg{txO M4L5c3NCVkeHUh?=
human KYSE-270 cell NHH2[4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoTjTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUK3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODN6N{Kg{txO M1PhOXNCVkeHUh?=
human CAL-39 cell NITrO5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVvJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuN|UzPCEQvF2= NWTpZYhwW0GQR1XS
human IST-SL1 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVnFVGwzUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py52M{O0PUDPxE1? MWfTRW5ITVJ?
human SF539 cell NV\LeGJbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVfJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz61OVE6PyEQvF2= MWLTRW5ITVJ?
human TE-1 cell MoGyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYrrfXVsUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{45QTB7MTFOwG0> NFiyZ3JUSU6JRWK=
human NB10 cell NVzBVYkxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3rEXGlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVg4PThizszN Mn22V2FPT0WU
human 8505C cell M3H6XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml3jTY5pcWKrdHnvckBw\iCqdX3hckA5PTB3QzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPVk5OTdizszN NID4UHNUSU6JRWK=
human DB cell NVzDRlBnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXmx[4NNUW6qaXLpeIlwdiCxZjDoeY1idiCGQjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNFEyOzFizszN NX3IUJVFW0GQR1XS
human NCI-H28 cell NWr1bWZ[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlOyTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42QTh6NDFOwG0> MWLTRW5ITVJ?
human LOXIMVI cell MlmyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4mycWlvcGmkaYTpc44hd2ZiaIXtZY4hVE:[SV3WTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPjN5MEGg{txO MnHqV2FPT0WU
human ME-180 cell M3rEXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHH0elRKdmirYnn0bY9vKG:oIHj1cYFvKE2HLUG4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvQDB|OUWg{txO NXHWcYJ4W0GQR1XS
human ES3 cell M{PpSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{HCdmlvcGmkaYTpc44hd2ZiaIXtZY4hTVN|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT6xNlc6PCEQvF2= MYjTRW5ITVJ?
human SK-N-FI cell NUDrWWNkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWjPXmRzUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWZKKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS52MU[5PUDPxE1? MUjTRW5ITVJ?
human NBsusSR cell MnzYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MljuTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOjNzODFOwG0> MnywV2FPT0WU
human MCF7 cell M3jXOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVPTRXMyUW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTh2OTFOwG0> NXm4UXZKW0GQR1XS
human A549 cell NFnHdVNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXPJcohq[mm2aX;uJI9nKGi3bXHuJGE2PDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yNFYh|ryP M2nVW3NCVkeHUh?=
human HuP-T4 cell M33qPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlQzPzFizszN M{D3VHNCVkeHUh?=
human HSC-2 cell MljwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYrvSGdIUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNDd6NEKg{txO MlrZV2FPT0WU
human A253 cell NIPUUYtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXHMVlVzUW6qaXLpeIlwdiCxZjDoeY1idiCDMkWzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh|NDFOwG0> NYq3O|ZxW0GQR1XS
human HCT-116 cell MmiwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYLJcohq[mm2aX;uJI9nKGi3bXHuJHBENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOD6wNFQ{KM7:TR?= M1;vTHNCVkeHUh?=
human HCT-116 cell MkLFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXLZRXpFUW6qaXLpeIlwdiCxZjDoeY1idiCKQ2StNVE3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTlwNk[4JO69VQ>? MkDqV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 for 24 h in indicated dose dependent manner, (F) 10 uM RO-3306 for 12 h up to 48 h. The expression level of the indicated protein and the phosphorylated form of H2A.x and p38 were examined via Western blot analyses.

27385216
Immunofluorescence
PARP1 / Plk1 ; 

PubMed: 28069876     


Representative images of IF staining for PARP1 and Plk1 in 22RV1 cells treated with 0.3 mM mimosine for 20 h, 4 mM hydroxyurea for 24 h, 10 µM RO-3306 for 7 h or 200 ng/ml nocodazole (Noc) for 12 h.

28069876
Growth inhibition assay
Cell viability; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 in indicated time- and dose-dependent manner. Cell viability were measured via crystal violet assay. Results are the means ± S.E. n = 3. 

27385216

Protocol

Kinase Assay:[1]
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CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
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  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC2=C(C=CC(=C2)C=C3C(=O)NC(=NCC4=CC=CS4)S3)N=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID