Ro-3306

Catalog No.S7747

Ro-3306 Chemical Structure

Molecular Weight(MW): 351.45

RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

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Cited by 15 Publications

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  • HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.

    J Cell Biol, 2015, 209(2): 221-34 . Ro-3306 purchased from Selleck.

  • A, Expression of IFIT3 in HeLa cells pretreated with 5 μM RO-3306 for 12 hours and then stimulated with type I IFN (1,000 units/ml) for 8 hours. The cells were collected and total RNA was extracted for the detection of IFIT3 mRNA. Results are the relative expression levels of IFIT3 mRNA normalized to endogenous GAPDH mRNA levels. B, Expression of IFI27 in HeLa cells measured in the same samples as in A.

    Arthritis Rheumatol, 2016, 68(5):1222-32.. Ro-3306 purchased from Selleck.

  • AML cells were treated with AZ20 in the absence or presence of RO-3306 (RO) for 24 h. Cells were then subjected to annexin V-FITC/PI staining and flow cytometry analyses. Combined treatment was compared to AZ20 treatment alone using pair-wise two-sample t-test. ***Indicates p < 0.001.

    Sci Rep, 2017, 7:41950. Ro-3306 purchased from Selleck.

  • Cell cycle synchronization in M phase. Cells were treated with 6 µM RO-3306 for 20 h. After release from RO-3306 treatment, the cells were fixed at 0.5, 1, and 1.5 h after the release. α-tubulin (green), DNA (red). Scale bars, 20 µm.

    Int J Mol Sci, 2018, 19(12). Ro-3306 purchased from Selleck.

  • (A). Cell toxicity of CDK1 Inhibitor (RO‐3306) or CDK6 (Palbociclib HCl) on Huh7 cells. (B). Flow cytometry analysis of the cell cycle by CDK1 inhibitor (5 μm) or CDK6 inhibitor (2.5 μm) treatment.

    FEBS Lett, 2018, 592(11):1893-1904. Ro-3306 purchased from Selleck.

Purity & Quality Control

Choose Selective CDK Inhibitors

Biological Activity

Description RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.
Targets
CDK1 [1]
(Cell-free assay)
PKCδ [1]
(Cell-free assay)
SGK [1]
(Cell-free assay)
ERK [1]
(Cell-free assay)
20 nM(Ki) 318 nM(Ki) 497 nM(Ki) 1980 nM(Ki)
In vitro

RO-3306 inhibits CDK1/cyclin B1, CDK1/cyclin A, CDK2/cyclin E, and CDK4/cyclin D activity with Ki of 35 nM, 110 nM, 340 nM, and over 2000 nM, respectively. Treatment of HCT116, SW480, and HeLa cells with RO-3306 for 20 h leads to a complete block of the cell cycle in the G2/M phase. The proliferation of both HCT116 and SW480 is effectively blocked by RO-3306. RO-3306 appears to be more proapoptotic in cancer cells (HCT116 and SW480) than nontumorigenic cells (MCF 10A and MCF 12A).[1] RO-3306 effectively arrests oocyte maturation at a concentration of 10 μM.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NCI-SNU-1 cell NYnqR3JST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX7TO2d2UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtV25WNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkezNFk2KM7:TR?= NF3tRnlUSU6JRWK=
human H460a cells NVThVlZCWHKxbHnm[ZJifGmxbjDhd5NigQ>? MUXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEh2NkDhJINmdGy|LDDJR|UxRTFwMESg{txO MnzhNVc{ODN2MkG=
human MDA435 cells M3raVnBzd2yrZnXyZZRqd25iYYPzZZk> NHjMPHRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERVQ{PSClZXzsd{whUUN3ME2xMlE1KM7:TR?= NWDHVWdkOTd|MEO0NlE>
human HCT116 cells NVzRWohzWHKxbHnm[ZJifGmxbjDhd5NigQ>? M4fXOmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzMEBKSzVyPUGuNVQh|ryP M{T4OlE4OzB|NEKx
human H4 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWLJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5zNUi3OUDPxE1? MWPTRW5ITVJ?
human PA-1 cell NWHj[Vd3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIS0cGdKdmirYnn0bY9vKG:oIHj1cYFvKFCDLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIzPjV2IN88US=> NFTzbYtUSU6JRWK=
human T98G cell NIj6T21Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlXvTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zOlgyOiEQvF2= MnvSV2FPT0WU
human SW1710 cell NXva[5diT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlE1ODhizszN NX;5bVhwW0GQR1XS
human U251 cell M136c2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX62V|NOUW6qaXLpeIlwdiCxZjDoeY1idiCXMkWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43Ozd5MTFOwG0> M1TrWnNCVkeHUh?=
human ALL-PO cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVGy[INvUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTFwOUC4NVMh|ryP MXTTRW5ITVJ?
human RKO cells MYHQdo9tcW[ncnH0bY9vKGG|c3H5 NUfoR5U4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDST28h[2WubIOsJGlEPTB;MjFOwG0> NEjTW24yPzNyM{SyNS=>
human SK-N-DZ cell NFfBOYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkD1TY5pcWKrdHnvckBw\iCqdX3hckBUUy2QLVTaJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4xOTh5NjFOwG0> MX\TRW5ITVJ?
human A4-Fuk cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml7QTY5pcWKrdHnvckBw\iCqdX3hckBCPC2IdXugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlA2PjB2IN88US=> NXzGRmtyW0GQR1XS
human SW480 cells NW\zSGVXWHKxbHnm[ZJifGmxbjDhd5NigQ>? M4jxR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e0PFAh[2WubIOsJGlEPTB;Mj6yJO69VQ>? Mn;zNVc{ODN2MkG=
human 639-V cell MnjFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2SzbGlvcGmkaYTpc44hd2ZiaIXtZY4hPjN7LW[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlI4PTN5IN88US=> MlfGV2FPT0WU
human Hs-578-T cell M3PnRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGnsWnBKdmirYnn0bY9vKG:oIHj1cYFvKEi|LUW3PE1VKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5{N{e5PUDPxE1? NXjxTYU6W0GQR1XS
human UM-UC-3 cell M1\Zb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIHLfoxKdmirYnn0bY9vKG:oIHj1cYFvKFWPLWXDMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5Nige,:jDDJR|UxRTJwN{O5N|Yh|ryP MUnTRW5ITVJ?
human BPH-1 cell NGexSWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2[zT2lvcGmkaYTpc44hd2ZiaIXtZY4hSlCKLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlk4PDV3IN88US=> M{HUcnNCVkeHUh?=
human 5637 cell M1flSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHLTWVBKdmirYnn0bY9vKG:oIHj1cYFvKDV4M{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlA5PTN{IN88US=> NGX1TXRUSU6JRWK=
human 8-MG-BA cell NEK4UHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXTtUo5GUW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD1|LkG0NFQ6KM7:TR?= NYq0UYlqW0GQR1XS
human HCT116 cells NH;ucIVEgXSxdH;4bYPDqGG|c3H5 M3vPfFUh\GG7cx?= MkPwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGGodHXyJFUh\GG7czDifUBOXFNiYYPzZZnwxIxiSVO1NF0{NjJizszN MVyyNlMzPjF4OB?=
human LB1047-RCC cell NVTEPHVRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn;1TY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{WwNUDPxE1? MVvTRW5ITVJ?
human KOSC-2 cell NYPMR|lJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3Pnb2lvcGmkaYTpc44hd2ZiaIXtZY4hU0:VQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{41PDZ4NkKg{txO M{L6c3NCVkeHUh?=
human COR-L88 cell M4qyZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH7pV3FKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{NjR5NUizJO69VQ>? MnXyV2FPT0WU
human PSN1 cell NHPRW2hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXPocYxzUW6qaXLpeIlwdiCxZjDoeY1idiCSU16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yODhyNTFOwG0> NWL2bFMyW0GQR1XS
human LXF-289 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWLCeWE4UW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC5{MUe2JO69VQ>? NXPv[3lQW0GQR1XS
human MN-60 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1nYXWlvcGmkaYTpc44hd2ZiaIXtZY4hVU5vNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlM4PjJ|IN88US=> NIfacIpUSU6JRWK=
human NEC8 cell NV\ZVXNLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4W4[GlvcGmkaYTpc44hd2ZiaIXtZY4hVkWFODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFcxPzZizszN MVrTRW5ITVJ?
human SJSA1 cells NFzzSIJRem:uaX\ldoF1cW:wIHHzd4F6 MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOMU1GxJINmdGy|LDDJR|UxRTRwNUWg{txO MWSxO|MxOzR{MR?=
human NCI-H2405 cell M1mzSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUnJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkSwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvPzR2OUOg{txO M{Lxd3NCVkeHUh?=
human YH-13 cell NY\3eGNGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml7TTY5pcWKrdHnvckBw\iCqdX3hckB[UC1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuO|U4PzZizszN MlH4V2FPT0WU
human SK-MEL-2 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYDJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4xQTZ|MjFOoI0> M3HBUnNCVkeHUh?=
human NB13 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NITBcZVKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlMzPzh4IN88US=> Mnv6V2FPT0WU
human GAMG cell Mn2yS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEfrem9KdmirYnn0bY9vKG:oIHj1cYFvKEeDTVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM4OTVizszN NIjsbJJUSU6JRWK=
human NCI-H1792 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4rQbWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixO|kzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS52N{m1OEDPxE1? NVnKNVhlW0GQR1XS
human KP-4 cell M{nEO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{PY[GlvcGmkaYTpc44hd2ZiaIXtZY4hU1BvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuOlk5OjNizszN MnPEV2FPT0WU
human HGC-27 cell Ml\NS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYjuVnRWUW6qaXLpeIlwdiCxZjDoeY1idiCKR1OtNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd|MEe3JO69VQ>? M4frTXNCVkeHUh?=
human GCT cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlP1TY5pcWKrdHnvckBw\iCqdX3hckBIS1RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lki1NVIyKM7:TR?= MUDTRW5ITVJ?
human PFSK-1 cell NVzVXpBjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFYyPjhizszN M4n1e3NCVkeHUh?=
human A172 cell NUflO3pXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MorTTY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT65NFc6KM7:TR?= NYrIXYNMW0GQR1XS
human HO-1-N-1 cell M3SxRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXfTWo43UW6qaXLpeIlwdiCxZjDoeY1idiCKTz2xMW4uOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUW0NlUh|ryP NYDSXlIxW0GQR1XS
human HN cell M{HnV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXr2U4VnUW6qaXLpeIlwdiCxZjDoeY1idiCKTjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPVcxQTdizszN NY\YOJVVW0GQR1XS
human IST-MES1 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\wU|dKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3NSXMyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS57N{WyNUDPxE1? NVv1cZhHW0GQR1XS
human TGW cell NX\Gd4ZLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3fQSWlvcGmkaYTpc44hd2ZiaIXtZY4hXEeZIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6wOVY5OiEQvF2= NW\jTHRJW0GQR1XS
human COR-L23 cell NYnZe5J6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoL4TY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEKzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{PzN3IN88US=> M{PiU3NCVkeHUh?=
human D-566MG cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlPRTY5pcWKrdHnvckBw\iCqdX3hckBFNTV4Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok44QDh5MzFOwG0> MUfTRW5ITVJ?
human SKG-IIIa cell NWXwdXRCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHHrUZNKdmirYnn0bY9vKG:oIHj1cYFvKFONRz3JTWliKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi56NUW3NkDPxE1? MXnTRW5ITVJ?
human NCI-H446 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVjnRYt4UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFQ1PiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEG4NUDPxE1? NUj1NWxDW0GQR1XS
human KYSE-270 cell M{nRZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWrJcohq[mm2aX;uJI9nKGi3bXHuJGt[W0VvMkewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{4xOzh5MjFOwG0> NFixTpVUSU6JRWK=
human CAL-39 cell NWTaOoJST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHXTXINKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2zPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOzV{NDFOwG0> MmOyV2FPT0WU
human IST-SL1 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVrJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlQ{OzR7IN88US=> NGXs[Y1USU6JRWK=
human SF539 cell MnzaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX;Jcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz61OVE6PyEQvF2= MWPTRW5ITVJ?
human TE-1 cell NXLQVWRZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWfEfXVJUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{45QTB7MTFOwG0> MoC2V2FPT0WU
human NB10 cell MmP3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIrXTYtKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlk5PzV6IN88US=> MWXTRW5ITVJ?
human 8505C cell MmjzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUHVclh[UW6qaXLpeIlwdiCxZjDoeY1idiB6NUC1R{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvQTl6MUeg{txO M{fr[3NCVkeHUh?=
human DB cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVrUNm96UW6qaXLpeIlwdiCxZjDoeY1idiCGQjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNFEyOzFizszN M3z6W3NCVkeHUh?=
human NCI-H28 cell NIrUbYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoPwTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42QTh6NDFOwG0> NVK1[284W0GQR1XS
human LOXIMVI cell M1TXOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NELJOJlKdmirYnn0bY9vKG:oIHj1cYFvKEyRWFnNWmkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjZ|N{CxJO69VQ>? NGW3cmxUSU6JRWK=
human ME-180 cell M1vzc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWDJcohq[mm2aX;uJI9nKGi3bXHuJG1GNTF6MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuPFA{QTVizszN NGKzcpdUSU6JRWK=
human ES3 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGTwdnRKdmirYnn0bY9vKG:oIHj1cYFvKEWVMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuNVI4QTRizszN M3HqUnNCVkeHUh?=
human SK-N-FI cell NIqwVY5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2nObWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3GTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPDF4OUmg{txO M{DIUnNCVkeHUh?=
human NBsusSR cell MmTDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWjYRZhSUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwMkOxPEDPxE1? M3;zWXNCVkeHUh?=
human MCF7 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF;DPFBKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yQDR7IN88US=> NVPDV3NIW0GQR1XS
human A549 cell M4\WVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{nrfGlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlIxPiEQvF2= NGSycZdUSU6JRWK=
human HuP-T4 cell NVzFc3lGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHTEW4xKdmirYnn0bY9vKG:oIHj1cYFvKEi3UD3UOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjR{N{Gg{txO NHP6cIdUSU6JRWK=
human HSC-2 cell M2HQc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIrLS2pKdmirYnn0bY9vKG:oIHj1cYFvKEiVQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEtPzh2MjFOwG0> NXf2PVhuW0GQR1XS
human A253 cell NWHle|lpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXW5OZJiUW6qaXLpeIlwdiCxZjDoeY1idiCDMkWzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh|NDFOwG0> NEDlSlNUSU6JRWK=
human HCT-116 cell M2HSTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKGi3bXHuJHBENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOD6wNFQ{KM7:TR?= M4DrSXNCVkeHUh?=
human HCT-116 cell NVn5UIdzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGhEXC1zMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPU43PjhizszN M2PFdnNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Cdk1 / Cdk1 / Cleaved PARP / Cleaved caspase-3 / p-p38 / Bad / Apaf1-1 / p-H2Ax / Bim / Bad; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 for 24 h in indicated dose dependent manner, (F) 10 uM RO-3306 for 12 h up to 48 h. The expression level of the indicated protein and the phosphorylated form of H2A.x and p38 were examined via Western blot analyses.

27385216
Immunofluorescence
PARP1 / Plk1 ; 

PubMed: 28069876     


Representative images of IF staining for PARP1 and Plk1 in 22RV1 cells treated with 0.3 mM mimosine for 20 h, 4 mM hydroxyurea for 24 h, 10 µM RO-3306 for 7 h or 200 ng/ml nocodazole (Noc) for 12 h.

28069876
Growth inhibition assay
Cell viability; 

PubMed: 27385216     


OVCA-429 and OVCAR-3 were exposed to RO-3306 in indicated time- and dose-dependent manner. Cell viability were measured via crystal violet assay. Results are the means ± S.E. n = 3. 

27385216

Protocol

Kinase Assay:[1]
+ Expand

CDK assay:

The activity of CDK1 cyclin B1, CDK1 cyclin A, CDK2 cyclin E, and CDK4 cyclin D is measured by a homogeneous time-resolved fluorescence assay in a 96-well format. The assay buffer contained 25 mM Hepes, 6.25 mM MgCl2, 0.003% Tween 20, 0.3 mg/mL BSA, 1.5 mM DTT, and ATP as follows: 162 μM (CDK1), 90 mM (CDK2), or 135 μM (CDK4). CDK1 and CDK2 buffer contained 10 mM MgCl2. Test compounds are diluted in assay buffer to 3-fold their final concentration in 20 μL, and the reaction is started by the addition of a 40 μL assay buffer containing the pRB substrate (0.185μM). The plates are incubated at 37°C for 30 min with constant agitation, and the reaction is terminated by the addition of 15 μL of 1.6μM anti-phospho pRB antibody (Ser-780) in 25 mM Hepes, 24 mM EDTA, and 0.2 mg/mL BSA. After an additional 30 min of incubation with shaking, 15μL of 3nM Lance-Eu-W1024-labeledanti-rabbitIgG and 60 nM Alophycocyanin-conjugated anti-His-6 antibody in 25 mM Hepes, and 0.5 mg/mL BSA is added and incubated for 1 h. The plates are read in the Victor-V multi- label reader at excitation 340 nm and emission 615 nm and 665 nm. The IC50 values are calculated from the readings at 665 nm and normalized for Europium readings at 615 nm. Ki values are calculated according to the equation: Ki= IC50/(1 + S/Km ), where S is the ATP concentration in the assay and Km is the Michaelis-Menten constant for ATP. The inhibitory activity against the panel of kinases is determined by the IMAP assay technology.
Cell Research:[3]
+ Expand
  • Cell lines: MDA-MB-231 cell line
  • Concentrations: 20 μM
  • Incubation Time: 72 h
  • Method: Log phases cells (25,000) are seeed in 96-well plates and incubated in a 37℃ incubator with CO2, After 24 h, different concentrations of RO-3306 are administered to determine the drug concentrations required to achieve a 50% growth inhibition (IC50). MTT (20 μL, 5mg/mL stock solution in saline) is added to each well and the cells are incubated for 4 h. Supernatants are removed and formazan crystals from viable cells are solubilized with 200 μL anhydrous DMSO. The absorbance is detected with a 550 model microplate reader at the 565 nm wavelength.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 13 mg/mL warmed (36.98 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 351.45
Formula

C18H13N3OS2

CAS No. 872573-93-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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CDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID