Senexin A

For research use only.

Catalog No.S8520

Senexin A Chemical Structure

CAS No. 1366002-50-7

Senexin A is a potent and selective inhibitor of CDK8 and its nearest relative, CDK19 with Kd values of 0.83 μM and 0.31 μM for CDK8 and CDK19 ATP site binding, respectively.

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Description Senexin A is a potent and selective inhibitor of CDK8 and its nearest relative, CDK19 with Kd values of 0.83 μM and 0.31 μM for CDK8 and CDK19 ATP site binding, respectively.
CDK19 [1]
(Cell-free assay)
CDK8 [1]
(Cell-free assay)
0.31 μM(Kd) 0.83 μM(Kd)
In vitro

p21 is shown to activate NF-κB–dependent transcription, and Senexin A inhibits p21-stimulated activity of the consensus NF-κB–dependent promoter. Senexin A has no effect on p21 induction by IPTG, on cell growth with or without p21, or on p21-induced senescent phenotype. Senexin A does not affect the inhibition of gene expression by p21 and does not interfere with p21-mediated inhibition of large sets of genes belonging to Gene Ontology (GO) categories of mitosis and DNA replication. Senexin A inhibits only p21-induced transcription but not other biological effects of p21. Senexin A inhibits CDK8 and CDK19 ATP site binding with Kd50 of 0.83 μM and 0.31 μM, respectively and CDK8 kinase activity with IC50 of 0.28 μM. Senexin A inhibits β-catenin–dependent transcription in HCT116 colon carcinoma cells. It does not inhibit ROCK and did not share cortistatin A's strong antiendothelial cell activity[1].

Methods Test Index PMID
Western blot
GLUT1 / GLUT3 / HK1 / HIF1A / CDK8 ; 

PubMed: 29117556     

Western blots showing levels of GLUT1, GLUT3, HK1, HIF1A, and CDK8 in HCT116 and SW480 cells under normoxic conditions, and treated with vehicle (DMSO) or 10 μM Senexin A.

In vivo The CDK8/19 inhibitor Senexin A reverses chemotherapy-induced paracrine tumor-promoting activities in vivo and does not inhibit reporter cell growth and showed no detectable toxicity in a mouse study[1].


Cell Research:


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  • Cell lines: MEF
  • Concentrations: 1 μM
  • Incubation Time: 24 h
  • Method:

    For conditioned media mitogenic assays, MEF, untreated or treated for 24 h with 200 nM doxorubicin, alone or in combination with 1 μM Senexin A, were washed and cultured for 48 h without drugs to collect conditioned media. The media were added to A549 cells, plated on 12-well plates at 104 cells per well; cells were counted after 48 h using the trypan blue exclusion assay. Experiments were performed in triplicate, and cells counted in at least three optical fields per experiment.

    (Only for Reference)
Animal Research:


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  • Animal Models: C57BL/6 mice
  • Dosages: 20 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (196.85 mM)
Ethanol 54 mg/mL (196.85 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 274.32


CAS No. 1366002-50-7
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C=C1)CCNC2=NC=NC3=C2C=C(C=C3)C#N

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID