Home Biosimilar Antibodies Apoptosis TNF-alpha inhibitor Infliximab (anti-TNF-alpha)
For research use only. Not for use in humans.

Infliximab (anti-TNF-alpha)

Infliximab (anti-TNF-alpha) is a purified, recombinant DNA-derived chimeric human-mouse IgG monoclonal antibody that consists of mouse heavy and light chain variable regions combined with human heavy and light chain constant regions. Infliximab neutralizes the biological activity of TNF-α by binding with high affinity to the soluble and transmembrane forms of TNF-α, and inhibits or prevents the effective binding of TNF-α with its receptors. MW=144.2 kDa.

Infliximab (anti-TNF-alpha)

5 Citations

The Nobel Prize

17 Nobel Prize winners have used Selleck products

Shimon Sakaguchi

Nature Communications 2025,16, Article number:1325

David Baker

Dev Cell 2023, 58(20):2163-2180.e9.

David Julius

Cell 2017, 185-198.e16

Michael Houghton

Cell Chem Biol, 2020, 27(7):780-792.e5

Charles M. Rice

Cell 2018, 172(3):423-438.e25

Quality Control

Batch: A201901 Purity: 97.9% Protein concentration: 5.00mg/ml Endotoxin Level: <1EU/mg
97.9

Specificity

Name Citation TNF-α TNF-alpha Others
QNZ (EVP4593) 156 NF-κB
JTE-607 Dihydrochloride 0 IL-1beta,IL-8,IL-6
PF-3644022 1 MK2
UCB-9260 0 NF-κB
MD2-TLR4-IN-1 0 TLR4,IL-6
R-7050 8 IL-1β
Resatorvid (TAK-242) 63 TLR4,IL-6,NO
Apigenin-7-O-glucuronide 0
UTL-5g 0
Benpyrine racemate 1
Amarogentin 0 TAS2R1,AMPK
Stylopine 0 IL-1β,NO,COX-2
Myrislignan 1 iNOS,IL-6,NF-κB
NE 52-QQ57 1 iNOS,IL-6,IL-1β
(3R,8S)-Falcarindiol 3 IL-1β,IL-6,iNOS
Gardenoside 0 NF-κB,P2X7 Receptor,IL-6
Cucurbitacin IIb 0 JNK,STAT3,NF-κB
IQ 3 1 IL-6,JNK3,JNK1
Isuzinaxib (APX-115 free base) 0 IL-6,MCP-1/CCL2,NOX2
AUDA 0 Smad3,TGF-β,IL-1β
CPI-1189 1
Madecassic acid 1 IL-1β,COX-2,iNOS
Homoplantaginin 1 IL-6,IKKβ,NF-κB
2',5'-Dihydroxyacetophenone 1 IL-6,NF-κB,ERK1/2
Forsythoside B 0 IκB,NF-κB,IL-6
Mulberroside A 0 ERK,NF-κB,Caspase-1
Ginsenoside Rb1 4 TLR3,NF-κB,IRAK-1
Muscone 4 IL-6,IL-1β,NLRP3 inflammasome
(±)-Shikonin 41 NF-κB,Proteasome,chemokine receptor
Methylthiouracil 0 IL-6,ERK1/2,NF-κB
Expand to Check More
1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description
Infliximab (anti-TNF-alpha) is a purified, recombinant DNA-derived chimeric human-mouse IgG monoclonal antibody that consists of mouse heavy and light chain variable regions combined with human heavy and light chain constant regions. Infliximab neutralizes the biological activity of TNF-α by binding with high affinity to the soluble and transmembrane forms of TNF-α, and inhibits or prevents the effective binding of TNF-α with its receptors. MW=144.2 kDa.
In vitro

Infliximab ameliorates TNF-alpha-induced insulin resistance in 3T3L1 adipocytes in vitro by restoring the insulin signalling pathway via PTP1B inhibition. Infliximab does not exacerbate production of inflammatory cytokines, and does not affect expression of TNFR, proliferation of ARPE-19, HTLV-1 proviral load, or apoptosis of ARPE-19. Infliximab does not exacerbate HTLV-1-related inflammation in the eye and represents an acceptable treatment option under HTLV-1 infectious conditions.
Cell Research

Objective: Glucose uptake assay
Cells: murine 3T3L1 cell line
Concentrations: 10 ng/ml
Incubation Time: 2 hr
Method: For glucose uptake assay, 3T3L1 mature adipocytes are in vitro cultured for 2 hr at 37°C in humidified 5% CO2 atmosphere, using 6-well cell-culture plates at a density of 30×104 cells per well. Adipocytes are stimulated twice at zero and 60 minutes with 2 μM insulin. In the infliximab-treated group, adipocytes are stimulated with 10 ng/ml infliximab at the beginning of the 2-hr in vitro assay.
Reference: https://pubmed.ncbi.nlm.nih.gov/30260514

Objective: Cell Counts
Cells: ARPE-19 cells, MT-2 cells, Jurkat cells
Concentrations: 10 μg/ml
Incubation Time: 24 h, 48 h, 72 h
Method: ARPE-19 cells (2 × 104) are co-cultured with three times the number of MT2 or Jurkat cells with or without Infliximab. After 0, 24, 48, or 72 h of co-culture, we remove the supernatants, trypsinized ARPE-19, and count the number of ARPE-19 cells under light microscopy.
Reference: https://pubmed.ncbi.nlm.nih.gov/31620105

Infliximab can apply to humanized mice, non-humanized mice (eg: C57BL/6 mice), peripheral blood and other related assays (Only for Reference)
In vivo

A single injection of infliximab in diabetic TNF-α(+/+) mice leads to suppression of the increased serum TNF-α and amelioration of the electrophysiological and biochemical deficits for at least 4 wk. Moreover, the increased TNF-α mRNA expression in diabetic DRG is also attenuated by infliximab, suggesting infliximab's effects may involve the local suppression of TNF-α. Infliximab, an agent currently in clinical use, is effective in targeting TNF-α action and expression and amelioration of diabetic neuropathy in mice. Mice given increasing doses of infliximab produces increasing levels of ADAs. Blood samples from mice given injections of human TNF and infliximab contains infliximab-TNF complexes.
Animal Research

Objective: To evaluate the effect on diabetic neuropathy
Animal Models: 8-week-old C57BL/6J (WT, TNF-α+/+) and TNF-α-deficient (TNFα−/−) mice of strain B6.129S6-Tnftm1Gkl/J in the C57BL/6 background
Formulation: Saline
Dosages: 10 μg/g
Administration: i.p.
Reference: https://pubmed.ncbi.nlm.nih.gov/21810933

Objective: pharmacokinetic (PK) study
Animal Models: 6–8 week-old female C57BL/6 mice
Formulation: --
Dosages: 0.3075 μg/g, 0.384 μg/g, 0.769 μg/g, 1.538 μg/g, 6.15 μg/g, 30.75 μg/g, 246 μg/g
Administration: i.p.
Reference: https://pubmed.ncbi.nlm.nih.gov/31401142

Infliximab can apply to humanized mice, non-humanized mice (eg: C57BL/6 mice), peripheral blood and other related assays (Only for Reference)
References
  • [4] https://pubmed.ncbi.nlm.nih.gov/31401142/

Product Details

CAS No. 170277-31-3
Molecular Weight 150 kDa
Isotype Chimeric IgG1
Source Chimeric (mouse/human)
Purification Protein G
Sterility 0.2 μM filtered
Formulation PBS, pH 7.0 Contains no stabilizers or preservatives
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

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