For research use only.
Catalog No.S1896 Synonyms: NCI-C04831, Hydroxycarbamide
CAS No. 127-07-1
Hydroxyurea (NSC-32065, NCI-C04831, Hydroxycarbamide) is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.
Selleck's Hydroxyurea (NSC-32065) has been cited by 21 publications
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|Description||Hydroxyurea (NSC-32065, NCI-C04831, Hydroxycarbamide) is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.|
hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants.hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase.
|In vitro||DMSO||15 mg/mL (197.23 mM)|
|Water||15 mg/mL (197.23 mM)|
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In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
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|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04808778||Recruiting||Drug: Hydroxyurea therapy per standard care||Sickle Cell Disease|Sickle Cell Anemia|Stroke Ischemic|Silent Stroke|Silent Cerebral Infarct|Stroke||Vanderbilt University Medical Center|Aminu Kano Teaching Hospital||May 17 2021||--|
|NCT04750707||Recruiting||Drug: Hydroxyurea||Sickle Cell Anemia in Children||Global Health Uganda LTD|Columbia University|University of Pittsburgh|Emory University|Mulago Hospital Uganda|Makerere University|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)||March 9 2021||Phase 3|
|NCT04691323||Recruiting||Other: HU-Go app||Sickle Cell Disease|Sickle B+ Thalassemia|Sickle Beta Zero Thalassemia|Sickle Cell Hemoglobin C||Ann & Robert H Lurie Children''s Hospital of Chicago|National Heart Lung and Blood Institute (NHLBI)||March 2 2021||Not Applicable|
|NCT04688411||Recruiting||Behavioral: MED-Go App||Sickle Cell Disease|Sickle Beta Zero Thalassemia|Sickle B+ Thalassemia|Sickle Cell Hemoglobin C||Ann & Robert H Lurie Children''s Hospital of Chicago|National Heart Lung and Blood Institute (NHLBI)||September 28 2020||Not Applicable|
|NCT04093986||Recruiting||Other: Chart Review|Other: Survey||Sickle Cell Disease|Sickle Cell Anemia||Children''s Hospital Medical Center Cincinnati|University of Connecticut|University of Colorado Denver|Guy''s and St Thomas'' NHS Foundation Trust|Duke University|Children''s Hospital of Philadelphia||December 22 2019||--|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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