Hydroxyurea

Catalog No.S1896 Synonyms: nci-c04831, nsc32065

Hydroxyurea Chemical Structure

Molecular Weight(MW): 76.05

Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.

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In DMSO USD 90 In stock
USD 70 In stock
USD 210 In stock
USD 470 In stock
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Cited by 6 Publications

2 Customer Reviews

  • Lethally irradiated C57BL/6 recipient mice were injected with a 1:1 mixture of GFP+JAK2(V671F) and wild-type bone marrow cells. Five weeks later, mice were treated with vehicle (C), hydroxyurea (H; 30 mg/kg twice daily IP), ruxolitinib (R; 30 mg/kg twice daily oral gavage), BMN673 (B; 0.33 mg/kg IV), H+R, H+B, R+B, and H+R+B for 3 weeks. Percentage of GFP+JAK2(V617F) was measured in (panel B) bone marrow cells, (panel C) splenocytes, and (panel D) peripheral blood leukocytes; (panel E) number of GFP+JAK2(V617F) Lin−Sca1+c-Kit+ (LSK) cells per 106 bone marrow cells was calculated, too. *P < .05, **P < .05, and ***P < .05 when compared with control, single treatment, and double treatment, respectively, from 6 to 7 mice using the Student t test.

    Blood, 2017, 130(26):2848-2859. Hydroxyurea purchased from Selleck.

    Cultured HCC827, H1650, and A549 cells were treated with DMSO and dynasore (50 μM) for 24 h or hydroxyurea (HU, 200 μM) for 12 h before lysed. Protein samples were analyzed by immunoblotting with indicated antibodies.

    Int J Biochem Cell Biol, 2018, 105:1-12. Hydroxyurea purchased from Selleck.

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Biological Activity

Description Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase.
Targets
ribonucleoside diphosphate reductase [1]
In vitro

hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants[1][2].hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 Leukemia cells MonkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHzWZVZEd26lZX70doF1cW:wIILldZVqemWmIH\vdkA2OCViaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCBqTEGyNVAhVGW3a3XtbYEqNCCLQ{WwQVIyNjN5OU[g{txO M3fRWVI6QTF3MkC=
P388 leukemic cell NGLIRWdRem:uaX\ldoF1cW:wIHHzd4F6 M{HZfVI1NTR6IHi= MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDv[kBkd22yb4Xu[EBmgHC{ZYPz[YQh[XNiY3;uZ4VvfHKjdHnvckB1cGG2IHnubIljcXS|IEWwKUBw\iCpcn;3eIghd2ZiUEO4PEBt\XWtZX3pZ{Bk\WyuIIP1d5BmdnOrb36g[pJwdSB{NDD0c{A1QCCqcjDh[pRmeiClb33wc5Vv\CCjZHTpeIlwdixiSVO1NF0{OiEQvF2= NE[yT|YzPzB7M{ey

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Protocol

Cell Research:[3]
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  • Cell lines: Erythroid cells
  • Concentrations: 30 μM
  • Incubation Time: 96 h
  • Method: Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped hydroxyurea treatment are treated with hydroxyurea in vitro.Treatment of cells performs in primary culture with 30 μM hydroxyurea for 96 hours.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Female athymic (nu/nu) nude mice(used for xenograft model)
  • Formulation: saline
  • Dosages: 1500 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 15 mg/mL (197.23 mM)
Water 15 mg/mL (197.23 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 76.05
Formula

CH4N2O2

CAS No. 127-07-1
Storage powder
in solvent
Synonyms nci-c04831, nsc32065

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04093986 Recruiting Other: Chart Review|Other: Survey Sickle Cell Disease|Sickle Cell Anemia Children''s Hospital Medical Center Cincinnati|University of Connecticut|University of Colorado Denver|Guy''s and St. Thomas'' Hospital|Duke University|Children''s Hospital of Philadelphia July 22 2019 --
NCT03789591 Recruiting Drug: Hydroxyurea Sickle Cell Disease|Sickle Cell Anemia Children''s Hospital Medical Center Cincinnati|Doris Duke Charitable Foundation January 17 2019 Phase 3
NCT03763656 Recruiting Drug: Hydroxy Urea Sickle Cell Disease|Sickle-Cell; Hemoglobin Disease Thalassemia|Sickle Cell-beta-thalassemia|Sickle Cell Hemoglobin C Nova Laboratories Limited November 20 2018 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID