Trans-ferulic acid

Trans-ferulic acid is a potent activator of AMPKunder high glucose condition.

Trans-ferulic acid Chemical Structure

Trans-ferulic acid Chemical Structure

CAS: 537-98-4

Purity & Quality Control

Batch: E065301 Purity: 99.88%
99.88

Trans-ferulic acid Related Products

Signaling Pathway

Choose Selective AMPK Inhibitors

Biological Activity

Description Trans-ferulic acid is a potent activator of AMPKunder high glucose condition.
Targets
AMPK [1]
In vitro
In vitro

Trans-ferulic acid can promote the activation of AMPK and the phosphorylation of acetyl-CoA carboxylase in hyperglycemia-induced human liver cells (HepG2) and rat skeletal muscle cells (L6).Trans-ferulic acid reduces the level of intracellular reactive oxygen species (ROS) and nitric oxide (NO) induced by hyperglycemia and subsequently increases the level of glutathione.[2]

Cell Research Cell lines Human liver cells (HepG2), rat skeletal muscle cells (L6),
Concentrations 10, 20, 30 μM
Incubation Time 24 h
Method

Fully-differentiated L6 myotubes and HepG2 cells are sub-cultured in six-well plates at a cell density of 2.5 x 105 cells/well and incubated in fresh DMEM containing 5 mM glucose for the control well and 25 mM glucose for the remaining wells for 24 hr. After incubation, the cells are treated separately in the serum-free media with 10, 20, and 30 μM concentrations of trans-ferulic acid and incubated further for 24 hr. Then, the cell extraction is used for western blot.

In Vivo
In vivo

TFA exhibits a potential protective effect againstf cisplatin induced testicular injury by inhibiting oxidative stress as well as TLR4/IRF3/INF-γ, P38-MAPK/NF-κB-p65/TNF-α, and JAK1/STAT-3/ERK1/2 inflammatory signaling pathways.[2]

Animal Research Animal Models Sprague–Dawley rats
Dosages 50 mg/kg
Administration p.o.

Chemical Information & Solubility

Molecular Weight 194.18 Formula

C10H10O4

CAS No. 537-98-4 SDF --
Smiles COC1=C(O)C=CC(=C1)\C=C\C(O)=O
Storage (From the date of receipt) 2 years -20°C liquid

In vitro
Batch:


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Trans-ferulic acid | Trans-ferulic acid supplier | purchase Trans-ferulic acid | Trans-ferulic acid cost | Trans-ferulic acid manufacturer | order Trans-ferulic acid | Trans-ferulic acid distributor