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A-769662

Name: A-769662
Brand: Selleck Chemicals
SKU: S2697
Rating: 5 (74 reviews)

Catalog No.S2697 Purity:99.67%

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

A-769662 Chemical Structure

CAS No. 844499-71-4

Purity & Quality Control

Batch: Purity: 99.67%

99.67

A-769662 Related Products

Related Targets	AMPK-related protein kinase	Click to Expand
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Related Compound Libraries	Kinase Inhibitor Library PI3K/Akt Inhibitor Library Apoptosis Compound Library Cell Cycle compound library NF-κB Signaling Compound Library	Click to Expand

Signaling Pathway

AMPK Signaling Pathway

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
mouse hepatocytes	Function assay	1 mM		DMSO	inhibits fatty acid synthesis with IC50 of 3.6 μM	16753576
rat hepatocytes	Function assay	1 mM		DMSO	inhibits fatty acid synthesis with IC50 of 3.6 μM	16753576
HEK293	Kinase assay	200 μM		DMSO	activates endogenous AMPK	17728241
CCL13	Kinase assay	200 μM		DMSO	activates endogenous AMPK	17728241
MEFs	Function assay	300 μM		DMSO	inhibits proteasomal function by an AMPK-independent mechanism	18593584
epididymal clear cells	Function assay	200 μM		DMSO	inhibits the pH-mediated V-ATPase accumulation at the apical membrane	19211918
3T3-L1	Function assay	1.2 mM		DMSO	inhibits 3T3-L1 Adipogenesis	19483304
3T3-L1	Function assay	1.2 mM		DMSO	inhibits the Expression of AdipogenesisRelated Transcription Factors and Markers	19483304
3T3-L1	Function assay	1.2 mM		DMSO	inhibits Mitotic Clonal Expansion	19483304
3T3-L1	cytotoxicity assay	1.2 mM		DMSO	decreases Cell Viability	19483304
3T3-L1	Kinase assay	1.2 mM		DMSO	activates AMPK	19483304
L6 skeletal muscle cells	Function assay	250 μM		DMSO	activates AMPK signaling pathways	19828836
L6 skeletal muscle cells	Function assay	250 μM		DMSO	inhibits the Na+-K+-ATPase transport activity and cell surface abundance	19828836
MDA-MB231	Apoptosis assay	400 μM		DMSO	sensitizes human breast cancer cell lines to TRAIL-induced apoptosis	19896469
BT474	Apoptosis assay	400 μM		DMSO	sensitizes human breast cancer cell lines to TRAIL-induced apoptosis	19896469
MCF7	Apoptosis assay	400 μM		DMSO	sensitizes human breast cancer cell lines to TRAIL-induced apoptosis	19896469
Mesenchymal stem cells	Kinase assay	10 µM		DMSO	induces a robust and sustained AMPK activation	24104879
Mesenchymal stem cells	cytotoxicity assay	100 µM		DMSO	decreases the MSC proliferation	24104879
MG-63	cytotoxicity assay	10 µM		DMSO	inhibits H2O2-Induced Osteoblast Cell Death	24960362
MC3T3-E1	cytotoxicity assay	10 µM		DMSO	inhibits H2O2-Induced Osteoblast Cell Death	24960362
MG-63	Apoptosis assay	10 µM		DMSO	suppresses H2O2-Induced Osteoblast Cell Apoptosis	24960362
MC3T3-E1	Apoptosis assay	10 µM		DMSO	suppresses H2O2-Induced Osteoblast Cell Apoptosis	24960362
MG-63	Function assay	10 µM		DMSO	alleviates ROS accumulation and ATP depletion caused by H2O2	24960362
MC3T3-E1	Function assay	10 µM		DMSO	alleviates ROS accumulation and ATP depletion caused by H2O2	24960362
MG-63	Function assay	10 µM		DMSO	facilitates H2O2-induced autophagy activation	24960362
MC3T3-E1	Function assay	10 µM		DMSO	facilitates H2O2-induced autophagy activation	24960362
PC3	Kinase assay	100 µM		DMSO	upregulates the levels of AMPK and ACC phosphorylation	25594043
PC3M	Kinase assay	100 µM		DMSO	upregulates the levels of AMPK and ACC phosphorylation	25594043
PC3	Function assay	100 µM		DMSO	induces PI3K/mTOR pathways	25594043
PC3M	Function assay	100 µM		DMSO	induces PI3K/mTOR pathways	25594043
PC3	Growth inhibitory assay	100 µM		DMSO	suppresses proliferation	25594043
PC3M	Growth inhibitory assay	100 µM		DMSO	suppresses proliferation	25594043
MC3T3-E1	Kinase assay	10 μM		DMSO	induces significant AMPK activation	26891866
MC3T3-E1	Growth inhibitory assay	10 μM		DMSO	inhibits Dex-induced osteoblast cell death	26891866
MC3T3-E1	Function assay	10 μM		DMSO	inhibits Dex-induced oxidative stress	26891866
BL21	Function assay		1 to 10 mins		Binding affinity to human recombinant N-terminal AVI-tagged phosphorylated AMPK alpha1beta1gamma1 expressed in Escherichia coli BL21 cells after 1 to 10 mins by biolayer interferometry, Kd = 0.51 μM.	26510622
BL21	Function assay		1 to 10 mins		Binding affinity to human recombinant N-terminal AVI-tagged phosphorylated AMPK alpha1beta2gamma1 expressed in Escherichia coli BL21 cells after 1 to 10 mins by biolayer interferometry, Kd = 14.5 μM.	26510622
HeLa	Function assay	100 uM	1 hr		Effect on AMPK activation in HeLa cells at 100 uM after 1 hr by immunoprecipitate kinase assay	17855357
HeLa	Function assay	100 uM	1 hr		Effect on AMPK phosphorylation in HeLa cells at 100 uM after 1 hr by immunoprecipitate kinase assay	17855357
HeLa	Function assay	100 uM	5 mins		Effect on AMPK phosphorylation in HeLa cells at 100 uM after 5 mins by immunoprecipitate kinase assay	17855357
HeLa	Function assay	100 uM	1 hr		Effect on acetyl-CoA carboxylase phosphorylation in HeLa cells at 100 uM after 1 hr by densitometry	17855357
HeLa	Function assay	100 uM	1 hr		Reduction of AMPK phosphorylation in HeLa cells at 100 uM after 1 hr in presence of STO-609 by immunoprecipitate kinase assay	17855357
HeLa	Function assay	100 uM	1 hr		Reduction of AMPK activation in HeLa cells at 100 uM after 1 hr in presence of STO-609 by immunoprecipitate kinase assay	17855357
HeLa	Function assay	100 uM	1 hr		Reduction of acetyl-CoA carboxylase phosphorylation in HeLa cells at 100 uM after 1 hr in presence of STO-609 by densitometry	17855357
CMK	Growth Inhibition Assay				Inhibition of human CMK cell growth in a cell viability assay, IC50 = 0.002202 μM.	SANGER
SIG-M5	Growth Inhibition Assay				Inhibition of human SIG-M5 cell growth in a cell viability assay, IC50 = 0.007856 μM.	SANGER
MV-4-11	Growth Inhibition Assay				Inhibition of human MV-4-11 cell growth in a cell viability assay, IC50 = 0.02274 μM.	SANGER
GDM-1	Growth Inhibition Assay				Inhibition of human GDM-1 cell growth in a cell viability assay, IC50 = 0.02553 μM.	SANGER
A431	Growth Inhibition Assay				Inhibition of human A431 cell growth in a cell viability assay, IC50 = 0.02919 μM.	SANGER
NALM-6	Growth Inhibition Assay				Inhibition of human NALM-6 cell growth in a cell viability assay, IC50 = 0.03166 μM.	SANGER
KM12	Growth Inhibition Assay				Inhibition of human KM12 cell growth in a cell viability assay, IC50 = 0.03213 μM.	SANGER
QIMR-WIL	Growth Inhibition Assay				Inhibition of human QIMR-WIL cell growth in a cell viability assay, IC50 = 0.03642 μM.	SANGER
NH-12	Growth Inhibition Assay				Inhibition of human NH-12 cell growth in a cell viability assay, IC50 = 0.03689 μM.	SANGER
KASUMI-1	Growth Inhibition Assay				Inhibition of human KASUMI-1 cell growth in a cell viability assay, IC50 = 0.0418 μM.	SANGER
ML-2	Growth Inhibition Assay				Inhibition of human ML-2 cell growth in a cell viability assay, IC50 = 0.04344 μM.	SANGER
YT	Growth Inhibition Assay				Inhibition of human YT cell growth in a cell viability assay, IC50 = 0.0467 μM.	SANGER
Daudi	Growth Inhibition Assay				Inhibition of human Daudi cell growth in a cell viability assay, IC50 = 0.04979 μM.	SANGER
OCI-AML2	Growth Inhibition Assay				Inhibition of human OCI-AML2 cell growth in a cell viability assay, IC50 = 0.0572 μM.	SANGER
ALL-PO	Growth Inhibition Assay				Inhibition of human ALL-PO cell growth in a cell viability assay, IC50 = 0.05793 μM.	SANGER
MOLT-16	Growth Inhibition Assay				Inhibition of human MOLT-16 cell growth in a cell viability assay, IC50 = 0.06191 μM.	SANGER
EHEB	Growth Inhibition Assay				Inhibition of human EHEB cell growth in a cell viability assay, IC50 = 0.06325 μM.	SANGER
AM-38	Growth Inhibition Assay				Inhibition of human AM-38 cell growth in a cell viability assay, IC50 = 0.0644 μM.	SANGER
C8166	Growth Inhibition Assay				Inhibition of human C8166 cell growth in a cell viability assay, IC50 = 0.06619 μM.	SANGER
RPMI-6666	Growth Inhibition Assay				Inhibition of human RPMI-6666 cell growth in a cell viability assay, IC50 = 0.06798 μM.	SANGER
BC-1	Growth Inhibition Assay				Inhibition of human BC-1 cell growth in a cell viability assay, IC50 = 0.06973 μM.	SANGER
CTV-1	Growth Inhibition Assay				Inhibition of human CTV-1 cell growth in a cell viability assay, IC50 = 0.07303 μM.	SANGER
HC-1	Growth Inhibition Assay				Inhibition of human HC-1 cell growth in a cell viability assay, IC50 = 0.08314 μM.	SANGER
JiyoyeP-2003	Growth Inhibition Assay				Inhibition of human JiyoyeP-2003 cell growth in a cell viability assay, IC50 = 0.08371 μM.	SANGER
RS4-11	Growth Inhibition Assay				Inhibition of human RS4-11 cell growth in a cell viability assay, IC50 = 0.08572 μM.	SANGER
PF-382	Growth Inhibition Assay				Inhibition of human PF-382 cell growth in a cell viability assay, IC50 = 0.0859 μM.	SANGER
G-361	Growth Inhibition Assay				Inhibition of human G-361 cell growth in a cell viability assay, IC50 = 0.08815 μM.	SANGER
LOXIMVI	Growth Inhibition Assay				Inhibition of human LOXIMVI cell growth in a cell viability assay, IC50 = 0.08833 μM.	SANGER
A3-KAW	Growth Inhibition Assay				Inhibition of human A3-KAW cell growth in a cell viability assay, IC50 = 0.09169 μM.	SANGER
EoL-1-cell	Growth Inhibition Assay				Inhibition of human EoL-1-cell cell growth in a cell viability assay, IC50 = 0.09558 μM.	SANGER
LOUCY	Growth Inhibition Assay				Inhibition of human LOUCY cell growth in a cell viability assay, IC50 = 0.09641 μM.	SANGER
HL-60	Growth Inhibition Assay				Inhibition of human HL-60 cell growth in a cell viability assay, IC50 = 0.09739 μM.	SANGER
MC116	Growth Inhibition Assay				Inhibition of human MC116 cell growth in a cell viability assay, IC50 = 0.10014 μM.	SANGER
CESS	Growth Inhibition Assay				Inhibition of human CESS cell growth in a cell viability assay, IC50 = 0.1011 μM.	SANGER
REH	Growth Inhibition Assay				Inhibition of human REH cell growth in a cell viability assay, IC50 = 0.10382 μM.	SANGER
HCC2157	Growth Inhibition Assay				Inhibition of human HCC2157 cell growth in a cell viability assay, IC50 = 0.10455 μM.	SANGER
HEL	Growth Inhibition Assay				Inhibition of human HEL cell growth in a cell viability assay, IC50 = 0.10728 μM.	SANGER
BL-70	Growth Inhibition Assay				Inhibition of human BL-70 cell growth in a cell viability assay, IC50 = 0.10805 μM.	SANGER
NCI-H1770	Growth Inhibition Assay				Inhibition of human NCI-H1770 cell growth in a cell viability assay, IC50 = 0.10865 μM.	SANGER
SK-MM-2	Growth Inhibition Assay				Inhibition of human SK-MM-2 cell growth in a cell viability assay, IC50 = 0.10944 μM.	SANGER
MONO-MAC-6	Growth Inhibition Assay				Inhibition of human MONO-MAC-6 cell growth in a cell viability assay, IC50 = 0.11372 μM.	SANGER
SR	Growth Inhibition Assay				Inhibition of human SR cell growth in a cell viability assay, IC50 = 0.11387 μM.	SANGER
MOLT-4	Growth Inhibition Assay				Inhibition of human MOLT-4 cell growth in a cell viability assay, IC50 = 0.11446 μM.	SANGER
KE-37	Growth Inhibition Assay				Inhibition of human KE-37 cell growth in a cell viability assay, IC50 = 0.12924 μM.	SANGER
ST486	Growth Inhibition Assay				Inhibition of human ST486 cell growth in a cell viability assay, IC50 = 0.13527 μM.	SANGER
HDLM-2	Growth Inhibition Assay				Inhibition of human HDLM-2 cell growth in a cell viability assay, IC50 = 0.13616 μM.	SANGER
ARH-77	Growth Inhibition Assay				Inhibition of human ARH-77 cell growth in a cell viability assay, IC50 = 0.1406 μM.	SANGER
EB2	Growth Inhibition Assay				Inhibition of human EB2 cell growth in a cell viability assay, IC50 = 0.14234 μM.	SANGER
CCRF-CEM	Growth Inhibition Assay				Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50 = 0.14576 μM.	SANGER
HD-MY-Z	Growth Inhibition Assay				Inhibition of human HD-MY-Z cell growth in a cell viability assay, IC50 = 0.14791 μM.	SANGER
BT-474	Growth Inhibition Assay				Inhibition of human BT-474 cell growth in a cell viability assay, IC50 = 0.15074 μM.	SANGER
KARPAS-45	Growth Inhibition Assay				Inhibition of human KARPAS-45 cell growth in a cell viability assay, IC50 = 0.16984 μM.	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay				Inhibition of human Ramos-2G6-4C10 cell growth in a cell viability assay, IC50 = 0.1715 μM.	SANGER
MC-CAR	Growth Inhibition Assay				Inhibition of human MC-CAR cell growth in a cell viability assay, IC50 = 0.17153 μM.	SANGER
MRK-nu-1	Growth Inhibition Assay				Inhibition of human MRK-nu-1 cell growth in a cell viability assay, IC50 = 0.17751 μM.	SANGER
MHH-PREB-1	Growth Inhibition Assay				Inhibition of human MHH-PREB-1 cell growth in a cell viability assay, IC50 = 0.18242 μM.	SANGER
SK-NEP-1	Growth Inhibition Assay				Inhibition of human SK-NEP-1 cell growth in a cell viability assay, IC50 = 0.18514 μM.	SANGER
BL-41	Growth Inhibition Assay				Inhibition of human BL-41 cell growth in a cell viability assay, IC50 = 0.18943 μM.	SANGER
CAS-1	Growth Inhibition Assay				Inhibition of human CAS-1 cell growth in a cell viability assay, IC50 = 0.19307 μM.	SANGER
NCI-H1522	Growth Inhibition Assay				Inhibition of human NCI-H1522 cell growth in a cell viability assay, IC50 = 0.19355 μM.	SANGER
CA46	Growth Inhibition Assay				Inhibition of human CA46 cell growth in a cell viability assay, IC50 = 0.19556 μM.	SANGER
L-363	Growth Inhibition Assay				Inhibition of human L-363 cell growth in a cell viability assay, IC50 = 0.21121 μM.	SANGER
NCI-H2087	Growth Inhibition Assay				Inhibition of human NCI-H2087 cell growth in a cell viability assay, IC50 = 0.21446 μM.	SANGER
GR-ST	Growth Inhibition Assay				Inhibition of human GR-ST cell growth in a cell viability assay, IC50 = 0.21764 μM.	SANGER
OPM-2	Growth Inhibition Assay				Inhibition of human OPM-2 cell growth in a cell viability assay, IC50 = 0.21791 μM.	SANGER
U-698-M	Growth Inhibition Assay				Inhibition of human U-698-M cell growth in a cell viability assay, IC50 = 0.21915 μM.	SANGER
CAL-148	Growth Inhibition Assay				Inhibition of human CAL-148 cell growth in a cell viability assay, IC50 = 0.22342 μM.	SANGER
L-428	Growth Inhibition Assay				Inhibition of human L-428 cell growth in a cell viability assay, IC50 = 0.22499 μM.	SANGER
DEL	Growth Inhibition Assay				Inhibition of human DEL cell growth in a cell viability assay, IC50 = 0.22825 μM.	SANGER
LU-134-A	Growth Inhibition Assay				Inhibition of human LU-134-A cell growth in a cell viability assay, IC50 = 0.23116 μM.	SANGER
MLMA	Growth Inhibition Assay				Inhibition of human MLMA cell growth in a cell viability assay, IC50 = 0.23399 μM.	SANGER
DOHH-2	Growth Inhibition Assay				Inhibition of human DOHH-2 cell growth in a cell viability assay, IC50 = 0.24513 μM.	SANGER
RPMI-8226	Growth Inhibition Assay				Inhibition of human RPMI-8226 cell growth in a cell viability assay, IC50 = 0.24596 μM.	SANGER
NCI-H2196	Growth Inhibition Assay				Inhibition of human NCI-H2196 cell growth in a cell viability assay, IC50 = 0.24776 μM.	SANGER
COR-L279	Growth Inhibition Assay				Inhibition of human COR-L279 cell growth in a cell viability assay, IC50 = 0.25293 μM.	SANGER
CAPAN-1	Growth Inhibition Assay				Inhibition of human CAPAN-1 cell growth in a cell viability assay, IC50 = 0.25589 μM.	SANGER
NOMO-1	Growth Inhibition Assay				Inhibition of human NOMO-1 cell growth in a cell viability assay, IC50 = 0.26322 μM.	SANGER
SUP-T1	Growth Inhibition Assay				Inhibition of human SUP-T1 cell growth in a cell viability assay, IC50 = 0.26516 μM.	SANGER
MEG-01	Growth Inhibition Assay				Inhibition of human MEG-01 cell growth in a cell viability assay, IC50 = 0.2687 μM.	SANGER
CHP-126	Growth Inhibition Assay				Inhibition of human CHP-126 cell growth in a cell viability assay, IC50 = 0.26909 μM.	SANGER
HCC1599	Growth Inhibition Assay				Inhibition of human HCC1599 cell growth in a cell viability assay, IC50 = 0.27841 μM.	SANGER
EB-3	Growth Inhibition Assay				Inhibition of human EB-3 cell growth in a cell viability assay, IC50 = 0.29293 μM.	SANGER
WSU-NHL	Growth Inhibition Assay				Inhibition of human WSU-NHL cell growth in a cell viability assay, IC50 = 0.29672 μM.	SANGER
COLO-684	Growth Inhibition Assay				Inhibition of human COLO-684 cell growth in a cell viability assay, IC50 = 0.3025 μM.	SANGER
LAMA-84	Growth Inhibition Assay				Inhibition of human LAMA-84 cell growth in a cell viability assay, IC50 = 0.30816 μM.	SANGER
TGW	Growth Inhibition Assay				Inhibition of human TGW cell growth in a cell viability assay, IC50 = 0.31095 μM.	SANGER
NCI-SNU-1	Growth Inhibition Assay				Inhibition of human NCI-SNU-1 cell growth in a cell viability assay, IC50 = 0.31106 μM.	SANGER
MHH-NB-11	Growth Inhibition Assay				Inhibition of human MHH-NB-11 cell growth in a cell viability assay, IC50 = 0.32291 μM.	SANGER
COLO-824	Growth Inhibition Assay				Inhibition of human COLO-824 cell growth in a cell viability assay, IC50 = 0.32838 μM.	SANGER
U-87-MG	Growth Inhibition Assay				Inhibition of human U-87-MG cell growth in a cell viability assay, IC50 = 0.33314 μM.	SANGER
HT	Growth Inhibition Assay				Inhibition of human HT cell growth in a cell viability assay, IC50 = 0.33482 μM.	SANGER
THP-1	Growth Inhibition Assay				Inhibition of human THP-1 cell growth in a cell viability assay, IC50 = 0.34164 μM.	SANGER
HCC2218	Growth Inhibition Assay				Inhibition of human HCC2218 cell growth in a cell viability assay, IC50 = 0.3418 μM.	SANGER
KARPAS-422	Growth Inhibition Assay				Inhibition of human KARPAS-422 cell growth in a cell viability assay, IC50 = 0.34433 μM.	SANGER
EM-2	Growth Inhibition Assay				Inhibition of human EM-2 cell growth in a cell viability assay, IC50 = 0.34443 μM.	SANGER
TUR	Growth Inhibition Assay				Inhibition of human TUR cell growth in a cell viability assay, IC50 = 0.34453 μM.	SANGER
MN-60	Growth Inhibition Assay				Inhibition of human MN-60 cell growth in a cell viability assay, IC50 = 0.34596 μM.	SANGER
DMS-153	Growth Inhibition Assay				Inhibition of human DMS-153 cell growth in a cell viability assay, IC50 = 0.37276 μM.	SANGER
J-RT3-T3-5	Growth Inhibition Assay				Inhibition of human J-RT3-T3-5 cell growth in a cell viability assay, IC50 = 0.37831 μM.	SANGER
EVSA-T	Growth Inhibition Assay				Inhibition of human EVSA-T cell growth in a cell viability assay, IC50 = 0.38095 μM.	SANGER
SHP-77	Growth Inhibition Assay				Inhibition of human SHP-77 cell growth in a cell viability assay, IC50 = 0.38298 μM.	SANGER
D-283MED	Growth Inhibition Assay				Inhibition of human D-283MED cell growth in a cell viability assay, IC50 = 0.38569 μM.	SANGER
GOTO	Growth Inhibition Assay				Inhibition of human GOTO cell growth in a cell viability assay, IC50 = 0.39536 μM.	SANGER
KMOE-2	Growth Inhibition Assay				Inhibition of human KMOE-2 cell growth in a cell viability assay, IC50 = 0.39664 μM.	SANGER
U-266	Growth Inhibition Assay				Inhibition of human U-266 cell growth in a cell viability assay, IC50 = 0.40452 μM.	SANGER
NCI-H226	Growth Inhibition Assay				Inhibition of human NCI-H226 cell growth in a cell viability assay, IC50 = 0.40703 μM.	SANGER
KG-1	Growth Inhibition Assay				Inhibition of human KG-1 cell growth in a cell viability assay, IC50 = 0.40876 μM.	SANGER
NCI-H1882	Growth Inhibition Assay				Inhibition of human NCI-H1882 cell growth in a cell viability assay, IC50 = 0.40912 μM.	SANGER
KMS-12-PE	Growth Inhibition Assay				Inhibition of human KMS-12-PE cell growth in a cell viability assay, IC50 = 0.41877 μM.	SANGER
KM-H2	Growth Inhibition Assay				Inhibition of human KM-H2 cell growth in a cell viability assay, IC50 = 0.42374 μM.	SANGER
NCI-H1417	Growth Inhibition Assay				Inhibition of human NCI-H1417 cell growth in a cell viability assay, IC50 = 0.43916 μM.	SANGER
RPMI-8402	Growth Inhibition Assay				Inhibition of human RPMI-8402 cell growth in a cell viability assay, IC50 = 0.44161 μM.	SANGER
EW-18	Growth Inhibition Assay				Inhibition of human EW-18 cell growth in a cell viability assay, IC50 = 0.45104 μM.	SANGER
HH	Growth Inhibition Assay				Inhibition of human HH cell growth in a cell viability assay, IC50 = 0.45751 μM.	SANGER
NCI-H526	Growth Inhibition Assay				Inhibition of human NCI-H526 cell growth in a cell viability assay, IC50 = 0.46847 μM.	SANGER
NEC8	Growth Inhibition Assay				Inhibition of human NEC8 cell growth in a cell viability assay, IC50 = 0.48029 μM.	SANGER
P12-ICHIKAWA	Growth Inhibition Assay				Inhibition of human P12-ICHIKAWA cell growth in a cell viability assay, IC50 = 0.48862 μM.	SANGER
DB	Growth Inhibition Assay				Inhibition of human DB cell growth in a cell viability assay, IC50 = 0.48893 μM.	SANGER
SJRH30	Growth Inhibition Assay				Inhibition of human SJRH30 cell growth in a cell viability assay, IC50 = 0.49478 μM.	SANGER
ATN-1	Growth Inhibition Assay				Inhibition of human ATN-1 cell growth in a cell viability assay, IC50 = 0.49906 μM.	SANGER
ECC4	Growth Inhibition Assay				Inhibition of human ECC4 cell growth in a cell viability assay, IC50 = 0.50901 μM.	SANGER
LU-65	Growth Inhibition Assay				Inhibition of human LU-65 cell growth in a cell viability assay, IC50 = 0.52007 μM.	SANGER
NCI-H1092	Growth Inhibition Assay				Inhibition of human NCI-H1092 cell growth in a cell viability assay, IC50 = 0.54078 μM.	SANGER
NCI-H187	Growth Inhibition Assay				Inhibition of human NCI-H187 cell growth in a cell viability assay, IC50 = 0.54464 μM.	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay				Inhibition of human LNCaP-Clone-FGC cell growth in a cell viability assay, IC50 = 0.5472 μM.	SANGER
LC4-1	Growth Inhibition Assay				Inhibition of human LC4-1 cell growth in a cell viability assay, IC50 = 0.56308 μM.	SANGER
NCI-H1436	Growth Inhibition Assay				Inhibition of human NCI-H1436 cell growth in a cell viability assay, IC50 = 0.59043 μM.	SANGER
NCI-H2171	Growth Inhibition Assay				Inhibition of human NCI-H2171 cell growth in a cell viability assay, IC50 = 0.62179 μM.	SANGER
LB647-SCLC	Growth Inhibition Assay				Inhibition of human LB647-SCLC cell growth in a cell viability assay, IC50 = 0.62241 μM.	SANGER
NCI-H2141	Growth Inhibition Assay				Inhibition of human NCI-H2141 cell growth in a cell viability assay, IC50 = 0.63266 μM.	SANGER
NCI-H1304	Growth Inhibition Assay				Inhibition of human NCI-H1304 cell growth in a cell viability assay, IC50 = 0.63388 μM.	SANGER
Raji	Growth Inhibition Assay				Inhibition of human Raji cell growth in a cell viability assay, IC50 = 0.66976 μM.	SANGER
NB69	Growth Inhibition Assay				Inhibition of human NB69 cell growth in a cell viability assay, IC50 = 0.68575 μM.	SANGER
NCI-H510A	Growth Inhibition Assay				Inhibition of human NCI-H510A cell growth in a cell viability assay, IC50 = 0.69172 μM.	SANGER
COR-L88	Growth Inhibition Assay				Inhibition of human COR-L88 cell growth in a cell viability assay, IC50 = 0.72456 μM.	SANGER
K052	Growth Inhibition Assay				Inhibition of human K052 cell growth in a cell viability assay, IC50 = 0.79127 μM.	SANGER
SK-N-DZ	Growth Inhibition Assay				Inhibition of human SK-N-DZ cell growth in a cell viability assay, IC50 = 0.80231 μM.	SANGER
KY821	Growth Inhibition Assay				Inhibition of human KY821 cell growth in a cell viability assay, IC50 = 0.81743 μM.	SANGER
MDA-MB-157	Growth Inhibition Assay				Inhibition of human MDA-MB-157 cell growth in a cell viability assay, IC50 = 0.82406 μM.	SANGER
MS-1	Growth Inhibition Assay				Inhibition of human MS-1 cell growth in a cell viability assay, IC50 = 0.82745 μM.	SANGER
L-540	Growth Inhibition Assay				Inhibition of human L-540 cell growth in a cell viability assay, IC50 = 0.84843 μM.	SANGER
MDA-MB-361	Growth Inhibition Assay				Inhibition of human MDA-MB-361 cell growth in a cell viability assay, IC50 = 0.86048 μM.	SANGER
NCI-SNU-5	Growth Inhibition Assay				Inhibition of human NCI-SNU-5 cell growth in a cell viability assay, IC50 = 0.94375 μM.	SANGER
KARPAS-299	Growth Inhibition Assay				Inhibition of human KARPAS-299 cell growth in a cell viability assay, IC50 = 0.94828 μM.	SANGER
NCI-H1694	Growth Inhibition Assay				Inhibition of human NCI-H1694 cell growth in a cell viability assay, IC50 = 0.9773 μM.	SANGER
P30-OHK	Growth Inhibition Assay				Inhibition of human P30-OHK cell growth in a cell viability assay, IC50 = 0.99292 μM.	SANGER
LU-165	Growth Inhibition Assay				Inhibition of human LU-165 cell growth in a cell viability assay, IC50 = 1.05606 μM.	SANGER
NB1	Growth Inhibition Assay				Inhibition of human NB1 cell growth in a cell viability assay, IC50 = 1.11241 μM.	SANGER
UACC-812	Growth Inhibition Assay				Inhibition of human UACC-812 cell growth in a cell viability assay, IC50 = 1.1488 μM.	SANGER
RL	Growth Inhibition Assay				Inhibition of human RL cell growth in a cell viability assay, IC50 = 1.16282 μM.	SANGER
NCI-H69	Growth Inhibition Assay				Inhibition of human NCI-H69 cell growth in a cell viability assay, IC50 = 1.19391 μM.	SANGER
SW1417	Growth Inhibition Assay				Inhibition of human SW1417 cell growth in a cell viability assay, IC50 = 1.23671 μM.	SANGER
NCI-H1155	Growth Inhibition Assay				Inhibition of human NCI-H1155 cell growth in a cell viability assay, IC50 = 1.27588 μM.	SANGER
NCI-H1581	Growth Inhibition Assay				Inhibition of human NCI-H1581 cell growth in a cell viability assay, IC50 = 1.44028 μM.	SANGER
MHH-CALL-2	Growth Inhibition Assay				Inhibition of human MHH-CALL-2 cell growth in a cell viability assay, IC50 = 1.48514 μM.	SANGER
SCLC-21H	Growth Inhibition Assay				Inhibition of human SCLC-21H cell growth in a cell viability assay, IC50 = 1.67837 μM.	SANGER
LK-2	Growth Inhibition Assay				Inhibition of human LK-2 cell growth in a cell viability assay, IC50 = 1.71089 μM.	SANGER
CPC-N	Growth Inhibition Assay				Inhibition of human CPC-N cell growth in a cell viability assay, IC50 = 1.76764 μM.	SANGER
SCC-3	Growth Inhibition Assay				Inhibition of human SCC-3 cell growth in a cell viability assay, IC50 = 2.04311 μM.	SANGER
NCI-H446	Growth Inhibition Assay				Inhibition of human NCI-H446 cell growth in a cell viability assay, IC50 = 2.05293 μM.	SANGER
RH-1	Growth Inhibition Assay				Inhibition of human RH-1 cell growth in a cell viability assay, IC50 = 2.13933 μM.	SANGER
NCI-H345	Growth Inhibition Assay				Inhibition of human NCI-H345 cell growth in a cell viability assay, IC50 = 2.14996 μM.	SANGER
A253	Growth Inhibition Assay				Inhibition of human A253 cell growth in a cell viability assay, IC50 = 2.25377 μM.	SANGER
DMS-79	Growth Inhibition Assay				Inhibition of human DMS-79 cell growth in a cell viability assay, IC50 = 2.36386 μM.	SANGER
SK-N-FI	Growth Inhibition Assay				Inhibition of human SK-N-FI cell growth in a cell viability assay, IC50 = 2.50034 μM.	SANGER
SK-MEL-1	Growth Inhibition Assay				Inhibition of human SK-MEL-1 cell growth in a cell viability assay, IC50 = 2.61066 μM.	SANGER
HuO-3N1	Growth Inhibition Assay				Inhibition of human HuO-3N1 cell growth in a cell viability assay, IC50 = 2.69128 μM.	SANGER
SIMA	Growth Inhibition Assay				Inhibition of human SIMA cell growth in a cell viability assay, IC50 = 2.72143 μM.	SANGER
SU-DHL-1	Growth Inhibition Assay				Inhibition of human SU-DHL-1 cell growth in a cell viability assay, IC50 = 2.90246 μM.	SANGER
P31-FUJ	Growth Inhibition Assay				Inhibition of human P31-FUJ cell growth in a cell viability assay, IC50 = 3.01209 μM.	SANGER
A101D	Growth Inhibition Assay				Inhibition of human A101D cell growth in a cell viability assay, IC50 = 3.23245 μM.	SANGER
RPMI-8866	Growth Inhibition Assay				Inhibition of human RPMI-8866 cell growth in a cell viability assay, IC50 = 3.58797 μM.	SANGER
NCI-H209	Growth Inhibition Assay				Inhibition of human NCI-H209 cell growth in a cell viability assay, IC50 = 3.59247 μM.	SANGER
JVM-2	Growth Inhibition Assay				Inhibition of human JVM-2 cell growth in a cell viability assay, IC50 = 3.62244 μM.	SANGER
LP-1	Growth Inhibition Assay				Inhibition of human LP-1 cell growth in a cell viability assay, IC50 = 3.79042 μM.	SANGER
IST-SL1	Growth Inhibition Assay				Inhibition of human IST-SL1 cell growth in a cell viability assay, IC50 = 3.91611 μM.	SANGER
TE-9	Growth Inhibition Assay				Inhibition of human TE-9 cell growth in a cell viability assay, IC50 = 3.99619 μM.	SANGER
NCI-H1618	Growth Inhibition Assay				Inhibition of human NCI-H1618 cell growth in a cell viability assay, IC50 = 4.17788 μM.	SANGER
COR-L105	Growth Inhibition Assay				Inhibition of human COR-L105 cell growth in a cell viability assay, IC50 = 4.51462 μM.	SANGER
KU-19-19	Growth Inhibition Assay				Inhibition of human KU-19-19 cell growth in a cell viability assay, IC50 = 4.56729 μM.	SANGER
HCC70	Growth Inhibition Assay				Inhibition of human HCC70 cell growth in a cell viability assay, IC50 = 5.75167 μM.	SANGER
DG-75	Growth Inhibition Assay				Inhibition of human DG-75 cell growth in a cell viability assay, IC50 = 6.18885 μM.	SANGER
CCF-STTG1	Growth Inhibition Assay				Inhibition of human CCF-STTG1 cell growth in a cell viability assay, IC50 = 6.74902 μM.	SANGER
SBC-1	Growth Inhibition Assay				Inhibition of human SBC-1 cell growth in a cell viability assay, IC50 = 6.88808 μM.	SANGER
CAL-85-1	Growth Inhibition Assay				Inhibition of human CAL-85-1 cell growth in a cell viability assay, IC50 = 7.45587 μM.	SANGER
BxPC-3	Growth Inhibition Assay				Inhibition of human BxPC-3 cell growth in a cell viability assay, IC50 = 7.56085 μM.	SANGER
YAPC	Growth Inhibition Assay				Inhibition of human YAPC cell growth in a cell viability assay, IC50 = 7.74286 μM.	SANGER
HCC1806	Growth Inhibition Assay				Inhibition of human HCC1806 cell growth in a cell viability assay, IC50 = 8.04507 μM.	SANGER
SK-MEL-28	Growth Inhibition Assay				Inhibition of human SK-MEL-28 cell growth in a cell viability assay, IC50 = 8.42441 μM.	SANGER
Ca9-22	Growth Inhibition Assay				Inhibition of human Ca9-22 cell growth in a cell viability assay, IC50 = 8.62748 μM.	SANGER
NCI-H889	Growth Inhibition Assay				Inhibition of human NCI-H889 cell growth in a cell viability assay, IC50 = 9.42457 μM.	SANGER
HT-3	Growth Inhibition Assay				Inhibition of human HT-3 cell growth in a cell viability assay, IC50 = 9.4754 μM.	SANGER
H4	Growth Inhibition Assay				Inhibition of human H4 cell growth in a cell viability assay, IC50 = 9.59864 μM.	SANGER
NCI-H2228	Growth Inhibition Assay				Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50 = 9.87581 μM.	SANGER
M14	Growth Inhibition Assay				Inhibition of human M14 cell growth in a cell viability assay, IC50 = 10.5224 μM.	SANGER
OVCAR-3	Growth Inhibition Assay				Inhibition of human OVCAR-3 cell growth in a cell viability assay, IC50 = 11.4748 μM.	SANGER
HCC1187	Growth Inhibition Assay				Inhibition of human HCC1187 cell growth in a cell viability assay, IC50 = 11.4776 μM.	SANGER
RT4	Growth Inhibition Assay				Inhibition of human RT4 cell growth in a cell viability assay, IC50 = 11.6532 μM.	SANGER
SW962	Growth Inhibition Assay				Inhibition of human SW962 cell growth in a cell viability assay, IC50 = 12.004 μM.	SANGER
BB49-HNC	Growth Inhibition Assay				Inhibition of human BB49-HNC cell growth in a cell viability assay, IC50 = 12.0436 μM.	SANGER
HCE-4	Growth Inhibition Assay				Inhibition of human HCE-4 cell growth in a cell viability assay, IC50 = 12.7585 μM.	SANGER
KU812	Growth Inhibition Assay				Inhibition of human KU812 cell growth in a cell viability assay, IC50 = 13.9562 μM.	SANGER
JAR	Growth Inhibition Assay				Inhibition of human JAR cell growth in a cell viability assay, IC50 = 14.113 μM.	SANGER
NCI-H2009	Growth Inhibition Assay				Inhibition of human NCI-H2009 cell growth in a cell viability assay, IC50 = 14.287 μM.	SANGER
HuO9	Growth Inhibition Assay				Inhibition of human HuO9 cell growth in a cell viability assay, IC50 = 14.4446 μM.	SANGER
U-118-MG	Growth Inhibition Assay				Inhibition of human U-118-MG cell growth in a cell viability assay, IC50 = 14.5013 μM.	SANGER
SH-4	Growth Inhibition Assay				Inhibition of human SH-4 cell growth in a cell viability assay, IC50 = 14.942 μM.	SANGER
ES5	Growth Inhibition Assay				Inhibition of human ES5 cell growth in a cell viability assay, IC50 = 15.3528 μM.	SANGER
NB6	Growth Inhibition Assay				Inhibition of human NB6 cell growth in a cell viability assay, IC50 = 15.4452 μM.	SANGER
COLO-829	Growth Inhibition Assay				Inhibition of human COLO-829 cell growth in a cell viability assay, IC50 = 15.6978 μM.	SANGER
NCI-H524	Growth Inhibition Assay				Inhibition of human NCI-H524 cell growth in a cell viability assay, IC50 = 15.751 μM.	SANGER
HPAF-II	Growth Inhibition Assay				Inhibition of human HPAF-II cell growth in a cell viability assay, IC50 = 16.1017 μM.	SANGER
GB-1	Growth Inhibition Assay				Inhibition of human GB-1 cell growth in a cell viability assay, IC50 = 16.5577 μM.	SANGER
LB771-HNC	Growth Inhibition Assay				Inhibition of human LB771-HNC cell growth in a cell viability assay, IC50 = 16.8228 μM.	SANGER
HuCCT1	Growth Inhibition Assay				Inhibition of human HuCCT1 cell growth in a cell viability assay, IC50 = 17.176 μM.	SANGER
SW837	Growth Inhibition Assay				Inhibition of human SW837 cell growth in a cell viability assay, IC50 = 17.3011 μM.	SANGER
NCI-N87	Growth Inhibition Assay				Inhibition of human NCI-N87 cell growth in a cell viability assay, IC50 = 17.5302 μM.	SANGER
BEN	Growth Inhibition Assay				Inhibition of human BEN cell growth in a cell viability assay, IC50 = 17.7386 μM.	SANGER
Mewo	Growth Inhibition Assay				Inhibition of human Mewo cell growth in a cell viability assay, IC50 = 17.8382 μM.	SANGER
MPP-89	Growth Inhibition Assay				Inhibition of human MPP-89 cell growth in a cell viability assay, IC50 = 18.2784 μM.	SANGER
NOS-1	Growth Inhibition Assay				Inhibition of human NOS-1 cell growth in a cell viability assay, IC50 = 18.3793 μM.	SANGER
Ca-Ski	Growth Inhibition Assay				Inhibition of human Ca-Ski cell growth in a cell viability assay, IC50 = 19.7889 μM.	SANGER
SW1088	Growth Inhibition Assay				Inhibition of human SW1088 cell growth in a cell viability assay, IC50 = 19.9447 μM.	SANGER
Capan-2	Growth Inhibition Assay				Inhibition of human Capan-2 cell growth in a cell viability assay, IC50 = 20.3327 μM.	SANGER
A498	Growth Inhibition Assay				Inhibition of human A498 cell growth in a cell viability assay, IC50 = 20.4092 μM.	SANGER
COLO-800	Growth Inhibition Assay				Inhibition of human COLO-800 cell growth in a cell viability assay, IC50 = 20.9232 μM.	SANGER
OVCAR-4	Growth Inhibition Assay				Inhibition of human OVCAR-4 cell growth in a cell viability assay, IC50 = 21.2508 μM.	SANGER
K-562	Growth Inhibition Assay				Inhibition of human K-562 cell growth in a cell viability assay, IC50 = 21.9049 μM.	SANGER
IA-LM	Growth Inhibition Assay				Inhibition of human IA-LM cell growth in a cell viability assay, IC50 = 22.3771 μM.	SANGER
SK-LMS-1	Growth Inhibition Assay				Inhibition of human SK-LMS-1 cell growth in a cell viability assay, IC50 = 22.4057 μM.	SANGER
CAL-51	Growth Inhibition Assay				Inhibition of human CAL-51 cell growth in a cell viability assay, IC50 = 23.0872 μM.	SANGER
NCI-H1651	Growth Inhibition Assay				Inhibition of human NCI-H1651 cell growth in a cell viability assay, IC50 = 24.1303 μM.	SANGER
GMS-10	Growth Inhibition Assay				Inhibition of human GMS-10 cell growth in a cell viability assay, IC50 = 24.4376 μM.	SANGER
NCI-H2347	Growth Inhibition Assay				Inhibition of human NCI-H2347 cell growth in a cell viability assay, IC50 = 24.6005 μM.	SANGER
MEL-JUSO	Growth Inhibition Assay				Inhibition of human MEL-JUSO cell growth in a cell viability assay, IC50 = 24.6906 μM.	SANGER
SW948	Growth Inhibition Assay				Inhibition of human SW948 cell growth in a cell viability assay, IC50 = 24.9296 μM.	SANGER
MKN45	Growth Inhibition Assay				Inhibition of human MKN45 cell growth in a cell viability assay, IC50 = 26.4465 μM.	SANGER
HCC1954	Growth Inhibition Assay				Inhibition of human HCC1954 cell growth in a cell viability assay, IC50 = 26.4502 μM.	SANGER
SAS	Growth Inhibition Assay				Inhibition of human SAS cell growth in a cell viability assay, IC50 = 26.4732 μM.	SANGER
SCC-25	Growth Inhibition Assay				Inhibition of human SCC-25 cell growth in a cell viability assay, IC50 = 26.5693 μM.	SANGER
MKN28	Growth Inhibition Assay				Inhibition of human MKN28 cell growth in a cell viability assay, IC50 = 26.7093 μM.	SANGER
T84	Growth Inhibition Assay				Inhibition of human T84 cell growth in a cell viability assay, IC50 = 26.7743 μM.	SANGER
HCT-15	Growth Inhibition Assay				Inhibition of human HCT-15 cell growth in a cell viability assay, IC50 = 26.9389 μM.	SANGER
DOK	Growth Inhibition Assay				Inhibition of human DOK cell growth in a cell viability assay, IC50 = 27.0723 μM.	SANGER
SK-MEL-24	Growth Inhibition Assay				Inhibition of human SK-MEL-24 cell growth in a cell viability assay, IC50 = 28.0766 μM.	SANGER
HCC1143	Growth Inhibition Assay				Inhibition of human HCC1143 cell growth in a cell viability assay, IC50 = 28.2552 μM.	SANGER
JEG-3	Growth Inhibition Assay				Inhibition of human JEG-3 cell growth in a cell viability assay, IC50 = 28.4578 μM.	SANGER
SF539	Growth Inhibition Assay				Inhibition of human SF539 cell growth in a cell viability assay, IC50 = 28.6049 μM.	SANGER
NB12	Growth Inhibition Assay				Inhibition of human NB12 cell growth in a cell viability assay, IC50 = 28.8033 μM.	SANGER
Hs-578-T	Growth Inhibition Assay				Inhibition of human Hs-578-T cell growth in a cell viability assay, IC50 = 29.1197 μM.	SANGER
HCT-116	Growth Inhibition Assay				Inhibition of human HCT-116 cell growth in a cell viability assay, IC50 = 29.4423 μM.	SANGER
EW-3	Growth Inhibition Assay				Inhibition of human EW-3 cell growth in a cell viability assay, IC50 = 29.5269 μM.	SANGER
639-V	Growth Inhibition Assay				Inhibition of human 639-V cell growth in a cell viability assay, IC50 = 29.8546 μM.	SANGER
IGR-1	Growth Inhibition Assay				Inhibition of human IGR-1 cell growth in a cell viability assay, IC50 = 30.0191 μM.	SANGER
LS-411N	Growth Inhibition Assay				Inhibition of human LS-411N cell growth in a cell viability assay, IC50 = 30.351 μM.	SANGER
D-502MG	Growth Inhibition Assay				Inhibition of human D-502MG cell growth in a cell viability assay, IC50 = 30.3725 μM.	SANGER
AN3-CA	Growth Inhibition Assay				Inhibition of human AN3-CA cell growth in a cell viability assay, IC50 = 31.4329 μM.	SANGER
RCC10RGB	Growth Inhibition Assay				Inhibition of human RCC10RGB cell growth in a cell viability assay, IC50 = 31.4464 μM.	SANGER
GT3TKB	Growth Inhibition Assay				Inhibition of human GT3TKB cell growth in a cell viability assay, IC50 = 31.7096 μM.	SANGER
BB30-HNC	Growth Inhibition Assay				Inhibition of human BB30-HNC cell growth in a cell viability assay, IC50 = 32.5217 μM.	SANGER
HT-144	Growth Inhibition Assay				Inhibition of human HT-144 cell growth in a cell viability assay, IC50 = 32.7065 μM.	SANGER
NCI-H1355	Growth Inhibition Assay				Inhibition of human NCI-H1355 cell growth in a cell viability assay, IC50 = 33.3495 μM.	SANGER
D-542MG	Growth Inhibition Assay				Inhibition of human D-542MG cell growth in a cell viability assay, IC50 = 33.3722 μM.	SANGER
NCI-H727	Growth Inhibition Assay				Inhibition of human NCI-H727 cell growth in a cell viability assay, IC50 = 33.4114 μM.	SANGER
NCI-H1755	Growth Inhibition Assay				Inhibition of human NCI-H1755 cell growth in a cell viability assay, IC50 = 33.5236 μM.	SANGER
KS-1	Growth Inhibition Assay				Inhibition of human KS-1 cell growth in a cell viability assay, IC50 = 33.6298 μM.	SANGER
SW626	Growth Inhibition Assay				Inhibition of human SW626 cell growth in a cell viability assay, IC50 = 33.7643 μM.	SANGER
MZ1-PC	Growth Inhibition Assay				Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50 = 33.8103 μM.	SANGER
NCI-H1650	Growth Inhibition Assay				Inhibition of human NCI-H1650 cell growth in a cell viability assay, IC50 = 34.326 μM.	SANGER
LCLC-97TM1	Growth Inhibition Assay				Inhibition of human LCLC-97TM1 cell growth in a cell viability assay, IC50 = 34.8526 μM.	SANGER
HTC-C3	Growth Inhibition Assay				Inhibition of human HTC-C3 cell growth in a cell viability assay, IC50 = 35.022 μM.	SANGER
SNU-387	Growth Inhibition Assay				Inhibition of human SNU-387 cell growth in a cell viability assay, IC50 = 35.2684 μM.	SANGER
IM-9	Growth Inhibition Assay				Inhibition of human IM-9 cell growth in a cell viability assay, IC50 = 35.5165 μM.	SANGER
KYSE-180	Growth Inhibition Assay				Inhibition of human KYSE-180 cell growth in a cell viability assay, IC50 = 35.6521 μM.	SANGER
HuH-7	Growth Inhibition Assay				Inhibition of human HuH-7 cell growth in a cell viability assay, IC50 = 35.6756 μM.	SANGER
CP66-MEL	Growth Inhibition Assay				Inhibition of human CP66-MEL cell growth in a cell viability assay, IC50 = 35.7968 μM.	SANGER
22RV1	Growth Inhibition Assay				Inhibition of human 22RV1 cell growth in a cell viability assay, IC50 = 35.8423 μM.	SANGER
SW48	Growth Inhibition Assay				Inhibition of human SW48 cell growth in a cell viability assay, IC50 = 36.2464 μM.	SANGER
COLO-205	Growth Inhibition Assay				Inhibition of human COLO-205 cell growth in a cell viability assay, IC50 = 37.2589 μM.	SANGER
MFH-ino	Growth Inhibition Assay				Inhibition of human MFH-ino cell growth in a cell viability assay, IC50 = 37.3918 μM.	SANGER
ECC12	Growth Inhibition Assay				Inhibition of human ECC12 cell growth in a cell viability assay, IC50 = 37.7142 μM.	SANGER
CAL-72	Growth Inhibition Assay				Inhibition of human CAL-72 cell growth in a cell viability assay, IC50 = 37.7221 μM.	SANGER
KNS-42	Growth Inhibition Assay				Inhibition of human KNS-42 cell growth in a cell viability assay, IC50 = 37.8215 μM.	SANGER
U031	Growth Inhibition Assay				Inhibition of human U031 cell growth in a cell viability assay, IC50 = 37.827 μM.	SANGER
EFE-184	Growth Inhibition Assay				Inhibition of human EFE-184 cell growth in a cell viability assay, IC50 = 37.8863 μM.	SANGER
TCCSUP	Growth Inhibition Assay				Inhibition of human TCCSUP cell growth in a cell viability assay, IC50 = 38.3341 μM.	SANGER
TE-11	Growth Inhibition Assay				Inhibition of human TE-11 cell growth in a cell viability assay, IC50 = 39.2614 μM.	SANGER
SK-MEL-3	Growth Inhibition Assay				Inhibition of human SK-MEL-3 cell growth in a cell viability assay, IC50 = 39.5577 μM.	SANGER
NCI-H1703	Growth Inhibition Assay				Inhibition of human NCI-H1703 cell growth in a cell viability assay, IC50 = 39.5618 μM.	SANGER
NCI-H748	Growth Inhibition Assay				Inhibition of human NCI-H748 cell growth in a cell viability assay, IC50 = 39.6102 μM.	SANGER
HuP-T3	Growth Inhibition Assay				Inhibition of human HuP-T3 cell growth in a cell viability assay, IC50 = 39.8492 μM.	SANGER
GCT	Growth Inhibition Assay				Inhibition of human GCT cell growth in a cell viability assay, IC50 = 40.2935 μM.	SANGER
LU-99A	Growth Inhibition Assay				Inhibition of human LU-99A cell growth in a cell viability assay, IC50 = 40.6473 μM.	SANGER
TGBC24TKB	Growth Inhibition Assay				Inhibition of human TGBC24TKB cell growth in a cell viability assay, IC50 = 40.7998 μM.	SANGER
NCI-H1993	Growth Inhibition Assay				Inhibition of human NCI-H1993 cell growth in a cell viability assay, IC50 = 41.1086 μM.	SANGER
769-P	Growth Inhibition Assay				Inhibition of human 769-P cell growth in a cell viability assay, IC50 = 41.226 μM.	SANGER
NCI-H2126	Growth Inhibition Assay				Inhibition of human NCI-H2126 cell growth in a cell viability assay, IC50 = 41.3099 μM.	SANGER
NCI-H1563	Growth Inhibition Assay				Inhibition of human NCI-H1563 cell growth in a cell viability assay, IC50 = 41.353 μM.	SANGER
NCI-H82	Growth Inhibition Assay				Inhibition of human NCI-H82 cell growth in a cell viability assay, IC50 = 41.4039 μM.	SANGER
SW1783	Growth Inhibition Assay				Inhibition of human SW1783 cell growth in a cell viability assay, IC50 = 41.6769 μM.	SANGER
NCI-H1648	Growth Inhibition Assay				Inhibition of human NCI-H1648 cell growth in a cell viability assay, IC50 = 41.9392 μM.	SANGER
A388	Growth Inhibition Assay				Inhibition of human A388 cell growth in a cell viability assay, IC50 = 42.1236 μM.	SANGER
SK-MEL-30	Growth Inhibition Assay				Inhibition of human SK-MEL-30 cell growth in a cell viability assay, IC50 = 42.3151 μM.	SANGER
EKVX	Growth Inhibition Assay				Inhibition of human EKVX cell growth in a cell viability assay, IC50 = 42.3726 μM.	SANGER
A172	Growth Inhibition Assay				Inhibition of human A172 cell growth in a cell viability assay, IC50 = 42.8624 μM.	SANGER
OMC-1	Growth Inhibition Assay				Inhibition of human OMC-1 cell growth in a cell viability assay, IC50 = 42.9399 μM.	SANGER
PANC-03-27	Growth Inhibition Assay				Inhibition of human PANC-03-27 cell growth in a cell viability assay, IC50 = 43.157 μM.	SANGER
NCI-H747	Growth Inhibition Assay				Inhibition of human NCI-H747 cell growth in a cell viability assay, IC50 = 43.3223 μM.	SANGER
HT55	Growth Inhibition Assay				Inhibition of human HT55 cell growth in a cell viability assay, IC50 = 43.7863 μM.	SANGER
BV-173	Growth Inhibition Assay				Inhibition of human BV-173 cell growth in a cell viability assay, IC50 = 43.7946 μM.	SANGER
LS-1034	Growth Inhibition Assay				Inhibition of human LS-1034 cell growth in a cell viability assay, IC50 = 43.9899 μM.	SANGER
NUGC-3	Growth Inhibition Assay				Inhibition of human NUGC-3 cell growth in a cell viability assay, IC50 = 44.0773 μM.	SANGER
IST-MEL1	Growth Inhibition Assay				Inhibition of human IST-MEL1 cell growth in a cell viability assay, IC50 = 44.2633 μM.	SANGER
8305C	Growth Inhibition Assay				Inhibition of human 8305C cell growth in a cell viability assay, IC50 = 44.31 μM.	SANGER
IMR-5	Growth Inhibition Assay				Inhibition of human IMR-5 cell growth in a cell viability assay, IC50 = 44.5743 μM.	SANGER
TE-6	Growth Inhibition Assay				Inhibition of human TE-6 cell growth in a cell viability assay, IC50 = 44.8539 μM.	SANGER
MZ2-MEL	Growth Inhibition Assay				Inhibition of human MZ2-MEL cell growth in a cell viability assay, IC50 = 45.0112 μM.	SANGER
RT-112	Growth Inhibition Assay				Inhibition of human RT-112 cell growth in a cell viability assay, IC50 = 45.4472 μM.	SANGER
SCC-9	Growth Inhibition Assay				Inhibition of human SCC-9 cell growth in a cell viability assay, IC50 = 45.5616 μM.	SANGER
SCC-4	Growth Inhibition Assay				Inhibition of human SCC-4 cell growth in a cell viability assay, IC50 = 45.5626 μM.	SANGER
CAL-54	Growth Inhibition Assay				Inhibition of human CAL-54 cell growth in a cell viability assay, IC50 = 45.6635 μM.	SANGER
LoVo	Growth Inhibition Assay				Inhibition of human LoVo cell growth in a cell viability assay, IC50 = 45.6839 μM.	SANGER
NCI-H358	Growth Inhibition Assay				Inhibition of human NCI-H358 cell growth in a cell viability assay, IC50 = 45.7286 μM.	SANGER
K5	Growth Inhibition Assay				Inhibition of human K5 cell growth in a cell viability assay, IC50 = 46.029 μM.	SANGER
LB1047-RCC	Growth Inhibition Assay				Inhibition of human LB1047-RCC cell growth in a cell viability assay, IC50 = 46.4256 μM.	SANGER
MES-SA	Growth Inhibition Assay				Inhibition of human MES-SA cell growth in a cell viability assay, IC50 = 46.5121 μM.	SANGER
UACC-257	Growth Inhibition Assay				Inhibition of human UACC-257 cell growth in a cell viability assay, IC50 = 46.5175 μM.	SANGER
NCI-H2342	Growth Inhibition Assay				Inhibition of human NCI-H2342 cell growth in a cell viability assay, IC50 = 46.8809 μM.	SANGER
TE-15	Growth Inhibition Assay				Inhibition of human TE-15 cell growth in a cell viability assay, IC50 = 46.8909 μM.	SANGER
SK-MES-1	Growth Inhibition Assay				Inhibition of human SK-MES-1 cell growth in a cell viability assay, IC50 = 47.3821 μM.	SANGER
NCI-H322M	Growth Inhibition Assay				Inhibition of human NCI-H322M cell growth in a cell viability assay, IC50 = 47.6561 μM.	SANGER
SK-OV-3	Growth Inhibition Assay				Inhibition of human SK-OV-3 cell growth in a cell viability assay, IC50 = 47.7452 μM.	SANGER
PANC-08-13	Growth Inhibition Assay				Inhibition of human PANC-08-13 cell growth in a cell viability assay, IC50 = 47.9641 μM.	SANGER
786-0	Growth Inhibition Assay				Inhibition of human 786-0 cell growth in a cell viability assay, IC50 = 48.073 μM.	SANGER
NCI-H460	Growth Inhibition Assay				Inhibition of human NCI-H460 cell growth in a cell viability assay, IC50 = 48.1255 μM.	SANGER
D-336MG	Growth Inhibition Assay				Inhibition of human D-336MG cell growth in a cell viability assay, IC50 = 48.4845 μM.	SANGER
SNG-M	Growth Inhibition Assay				Inhibition of human SNG-M cell growth in a cell viability assay, IC50 = 48.6701 μM.	SANGER
SK-CO-1	Growth Inhibition Assay				Inhibition of human SK-CO-1 cell growth in a cell viability assay, IC50 = 49.0025 μM.	SANGER
CAL-27	Growth Inhibition Assay				Inhibition of human CAL-27 cell growth in a cell viability assay, IC50 = 49.5334 μM.	SANGER
8505C	Growth Inhibition Assay				Inhibition of human 8505C cell growth in a cell viability assay, IC50 = 49.5364 μM.	SANGER
MCF7	Growth Inhibition Assay				Inhibition of human MCF7 cell growth in a cell viability assay, IC50 = 49.5865 μM.	SANGER
T47D	Growth Inhibition Assay				Inhibition of human T47D cell growth in a cell viability assay, IC50 = 49.7007 μM.	SANGER
YKG-1	Growth Inhibition Assay				Inhibition of human YKG-1 cell growth in a cell viability assay, IC50 = 49.9412 μM.	SANGER
Click to View More Cell Line Experimental Data

Biological Activity

Description

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

Targets

AMPK ^[1] (Cell-free assay)	Fatty acid synthesis ^[1] (in primary rat hepatocytes)
0.8 μM(EC50)	3.2 μM

In vitro
In vitro	A-769662 stimulates partially purified rat liver AMPK with EC50 with 0.8 μM. A-769662 activates AMPK purified from multiple tissues and species in a dose-responsive manner with modest variations in observed EC50s. EC50s determined for A-769662 using partially purified AMPK extracts from rat heart, rat muscle, or human embryonic kidney cells (HEKs) are 2.2 mM, 1.9 mM, or 1.1 mM, respectively. A 4 hours treatment of primary rat hepatocytes with A-769662 dose-dependently increases ACC phosphorylation, which correlated inhibition of fatty acid synthesis with IC50 of 3.2 μM. A-769662 also inhibits fatty acid sythesis in mouse hepatocytes with IC50 with 3.6 μM ^[1] A-769662 activates AMPK both allosterically and by inhibiting dephosphorylation of AMPK on Thr-172, similar to the effects of AMP. ^[2] A-769662 inhibits proteasomal function by an AMPK-independent mechanism. A-769662 affects the in vitro activity of purified 26S proteasomes but not the in vitro activity of purified 20S proteasomes. A-769662 has toxic effects on MEF cells. ^[3] A recent research shows A-769662 inhibited cell proliferation and DNA synthesis. ^[4]
Kinase Assay	96-well AMPK assay
Kinase Assay	AMPK activity is measured by monitoring phosphorylation of the SAMS peptide substrate (20 μM in standard assays and 100 μM in additivity assays) following a previously described protocol (Anderson et al., 2004). To determine whether A-769662-induced AMPK activation occurs in a reversible manner, AMP or A-769662 are preincubated with rat liver AMPK for 10 minutes at 20 times standard assay concentrations prior to dilution and measurement of AMPK activity. ^[1]
Cell Research	Cell lines	MEF cells
	Concentrations	300 μM
	Incubation Time	24 hours
	Method	Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective.
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Western blot	pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin p-AMPK / AMPK / p-S6K / S6K / p-ACC		26431377

In Vivo
In vivo	Short-term treatment of normal Sprague Dawley rats with A-769662 decreases liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreases hepatic expression of PEPCK, G6Pase, and FAS, lowers plasma glucose by 40%, reduced body weight gain and significantly decreases both plasma and liver triglyceride levels. ^[1]
Animal Research	Animal Models	Sprague Dawley rats
	Dosages	30 mg/kg
	Administration	Administered via i.p.

References

[4] Pevton KJ, et al, J Pharmacol Exp Ther, 2012, Jun 13.

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Chemical Information & Solubility

Molecular Weight	360.39	Formula	C₂₀H₁₂N₂O₃S
CAS No.	844499-71-4	SDF	Download A-769662 SDF
Smiles	C1=CC=C(C(=C1)C2=CC=C(C=C2)C3=CSC4=C3C(=C(C(=O)N4)C#N)O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 72 mg/mL ( (199.78 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	6.000mg/ml (16.65mM)	Taking the 1 mL working solution as an example, add 50 μL of 120 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (13.87mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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