A-769662

Name: A-769662
Brand: Selleck Chemicals
SKU: S2697
Rating: 5 (56 reviews)

For research use only.

Catalog No.S2697

56 publications

CAS No. 844499-71-4

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

Selleck's A-769662 has been cited by 56 publications

cell, 2019, 178(5):1102-1114

PubMed: 31442403 ( click the link to review the publication )

Cell Metab, 2019, 30(3):508-524

PubMed: 31204282 ( click the link to review the publication )

Cell Metab, 2019, 29(6):1350-1362

PubMed: 30982734 ( click the link to review the publication )

Mol Cell, 2021, S1097-2765(21)00232-X

PubMed: 33909988 ( click the link to review the publication )

Mol Cell, 2021, 81(18):3803-3819.e7

PubMed: 34547240 ( click the link to review the publication )

Nat Commun, 2021, 12(1):5058

PubMed: 34433808 ( click the link to review the publication )

Theranostics, 2021, 11(3):1079-1099

PubMed: 33391522 ( click the link to review the publication )

Cell Death Differ, 2021, 10.1038/s41418-021-00777-0

PubMed: 34040167 ( click the link to review the publication )

Cell Death Dis, 2021, 12(4):385

PubMed: 33837179 ( click the link to review the publication )

Mol Cancer Res, 2021, molcanres.0808.2020

PubMed: 33980612 ( click the link to review the publication )

STAR Protoc, 2021, 2(3):100496

PubMed: 34337440 ( click the link to review the publication )

Nat Metab, 2021, 10.1038/s42255-021-00479-4

PubMed: 34580494 ( click the link to review the publication )

Mol Cell, 2020, S1097-2765(20)30900-X

PubMed: 33400924 ( click the link to review the publication )

Mol Cell, 2020, S1097-2765(20)30786-3

PubMed: 33271062 ( click the link to review the publication )

FASEB J, 2020, 34(3):4702-4717

PubMed: 32030825 ( click the link to review the publication )

J Lipid Res, 2020, 10.1194/jlr.RA119000542

PubMed: 31964763 ( click the link to review the publication )

Front Oncol, 2020, 10:956

PubMed: 32596161 ( click the link to review the publication )

Am J Physiol Cell Physiol, 2020, 1;318(5):C1018-C1029

PubMed: 32293932 ( click the link to review the publication )

Cell Biosci, 2020, 10:79

PubMed: 32549974 ( click the link to review the publication )

Oxid Med Cell Longev, 2020, 2020:8708236

PubMed: 32104542 ( click the link to review the publication )

Cell Res, 2019, 29(6):460-473

PubMed: 30948787 ( click the link to review the publication )

Mol Cell, 2019, 10.1016/j.molcel.2019.09.007

PubMed: 31586547 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):192

PubMed: 31088566 ( click the link to review the publication )

Sci Rep, 2019, 9(1):1897

PubMed: 30760778 ( click the link to review the publication )
Cell Signal, 2019, 57:45-57

PubMed: 30772465 ( click the link to review the publication )

Am J Physiol Renal Physiol, 2019, 317(6):F1513-F1525

PubMed: 31566435 ( click the link to review the publication )

BMC Complement Altern Med, 2019, 19(1):255

PubMed: 31519174 ( click the link to review the publication )

Biol Pharm Bull, 2019, 42(8):1303-1309

PubMed: 31366866 ( click the link to review the publication )

Cell Rep, 2019, 29(6):1524-1538

PubMed: 31693893 ( click the link to review the publication )

Mol Cell, 2018, 69(2):279-291

PubMed: 29351847 ( click the link to review the publication )

Cancer Sci, 2018, 109(5):1468-1479

PubMed: 29516572 ( click the link to review the publication )

J Biol Chem, 2018, 293(29):11612-11624

PubMed: 29858246 ( click the link to review the publication )

Food Chem Toxicol, 2018, 122:143-150

PubMed: 30316840 ( click the link to review the publication )

Front Mol Neurosci, 2018, 11:98

PubMed: 29666569 ( click the link to review the publication )

Integr Cancer Ther, 2018, 17(2):511-523

PubMed: 28627240 ( click the link to review the publication )

Nat Cell Biol, 2017, 19(6):724-731

PubMed: 28553939 ( click the link to review the publication )

J Crohns Colitis, 2017, 11(12):1480-1490

PubMed: 28961920 ( click the link to review the publication )

J Exp Clin Cancer Res, 2017, 36(1):190

PubMed: 29273065 ( click the link to review the publication )

Sci Rep, 2017, 7(1):12891

PubMed: 29018223 ( click the link to review the publication )

Sci Rep, 2017, 7(1):16373

PubMed: 29180765 ( click the link to review the publication )

Biochem Biophys Res Commun, 2017, 492(3):520-527

PubMed: 28807827 ( click the link to review the publication )

Mol Med Rep, 2017, 16(3):3648-3656

PubMed: 28765969 ( click the link to review the publication )

Autophagy, 2016, 12(2):432-8

PubMed: 26902588 ( click the link to review the publication )

Sci Rep, 2016, 6:36721

PubMed: 27830724 ( click the link to review the publication )

Am J Physiol Endocrinol Metab, 2016, 311(4):E706-E719

PubMed: 27577855 ( click the link to review the publication )

Cell Rep, 2015, 12(4):599-609

PubMed: 26190112 ( click the link to review the publication )

Apoptosis, 2015, 20(10):1373-87

PubMed: 26276035 ( click the link to review the publication )

Cell Signal, 2015, 27(5):978-88

PubMed: 25683918 ( click the link to review the publication )
Cell Signal, 2015, 27(5):978-88

PubMed: ( click the link to review the publication )

Can J Physiol Pharmacol, 2015, 93(7):585-95

PubMed: 26120822 ( click the link to review the publication )

Cancer Res, 2014, 74(1):298-308

PubMed: 24240701 ( click the link to review the publication )

Pharmacol Res, 2014, 81:34-43

PubMed: 24508566 ( click the link to review the publication )

J Lipid Res, 2014, 55(7):1254-1266

PubMed: 24864274 ( click the link to review the publication )

Lung Cancer, 2014, 86(2):151-7

PubMed: 25240516 ( click the link to review the publication )

Am J Physiol Endocrinol Metab, 2014, 306(6):E688-96

PubMed: 24425763 ( click the link to review the publication )

Biochem Biophys Res Commun, 2013, 437(1):1-6

PubMed: 23743198 ( click the link to review the publication )

Purity & Quality Control

Choose Selective AMPK Inhibitors

Biological Activity

Description

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

Targets

AMPK ^[1] (Cell-free assay)	Fatty acid synthesis ^[1] (in primary rat hepatocytes)
0.8 μM(EC50)	3.2 μM

In vitro

A-769662 stimulates partially purified rat liver AMPK with EC50 with 0.8 μM. A-769662 activates AMPK purified from multiple tissues and species in a dose-responsive manner with modest variations in observed EC50s. EC50s determined for A-769662 using partially purified AMPK extracts from rat heart, rat muscle, or human embryonic kidney cells (HEKs) are 2.2 mM, 1.9 mM, or 1.1 mM, respectively. A 4 hours treatment of primary rat hepatocytes with A-769662 dose-dependently increases ACC phosphorylation, which correlated inhibition of fatty acid synthesis with IC50 of 3.2 μM. A-769662 also inhibits fatty acid sythesis in mouse hepatocytes with IC50 with 3.6 μM ^[1] A-769662 activates AMPK both allosterically and by inhibiting dephosphorylation of AMPK on Thr-172, similar to the effects of AMP. ^[2] A-769662 inhibits proteasomal function by an AMPK-independent mechanism. A-769662 affects the in vitro activity of purified 26S proteasomes but not the in vitro activity of purified 20S proteasomes. A-769662 has toxic effects on MEF cells. ^[3] A recent research shows A-769662 inhibited cell proliferation and DNA synthesis. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
mouse hepatocytes	MXLGeY5kfGmxbjDhd5NigQ>?	NUjFTpB[OSCvTR?=		NFziOJJFVVOR	M17rOolvcGmkaYTzJIZifHS7IHHjbYQhe3mwdHjld4l{KHerdHigTWM2OCCxZjCzMlYh\|ryP	M1y1W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{WzOVc3Lz5zNke1N\|U4PjxxYU6=
rat hepatocytes	NXS5e4w5TnWwY4Tpc44h[XO\|YYm=	MYixJI1O		Mn\CSG1UVw>?	MoLTbY5pcWKrdIOg[oF1fHliYXPp[EB{gW62aHXzbZMhf2m2aDDJR\|UxKG:oIEOuOkDPxE1?	MonQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5NUO1O\|YoRjF4N{WzOVc3RC:jPh?=
HEK293	MWDLbY5ie2ViYYPzZZk>	MoLTNlAxKM7:TR?=		NETlPHRFVVOR	MnvOZYN1cX[jdHXzJIVv\G:pZX7veZMhSU2SSx?=	M2jwPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K4NlQyLz5zN{eyPFI1OTxxYU6=
CCL13	M1rq[GtqdmG\|ZTDhd5NigQ>?	NGn3WoQzODBizszN		NHv4Z3hFVVOR	MX;hZ5RqfmG2ZYOg[Y5ld2enbn;1d{BCVVCN	Moq1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd5MkiyOFEoRjF5N{K4NlQyRC:jPh?=
MEFs	M4GxZ2Z2dmO2aX;uJIF{e2G7	NV;ZXZhtOzByIN88US=>		NGTiRZZFVVOR	NHPEVotqdmirYnn0d{Bxem:2ZXHzc41idCCodX7jeIlwdiCkeTDhckBCVVCNLXnu[IVx\W6mZX70JI1m[2ijbnnzcS=>	M17vZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUmzOVg1Lz5zOEW5N\|U5PDxxYU6=
epididymal clear cells	MlPYSpVv[3Srb36gZZN{[Xl?	M4jMfFIxOCEQvF2=		M2XqOmROW09?	MYrpcohq[mm2czD0bIUheEhvbXXkbYF1\WRiVj3BWHBie2ViYXPjeY12dGG2aX;uJIF1KHSqZTDhdIlk[WxibXXtZpJidmV?	M1XkSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkGxPVE5Lz5zOUKxNVkyQDxxYU6=
3T3-L1	NHXXW2dHfW6ldHnvckBie3OjeR?=	MYKxMlIhdU1?		NX3EeHplTE2VTx?=	NV7YW2xEcW6qaXLpeJMhO1R\|LVyxJGFlcXCxZ3Xu[ZNqew>?	NY\mWJl1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0PFM{ODRpPkG5OFg{OzB2PD;hQi=>
3T3-L1	NFvBdGhHfW6ldHnvckBie3OjeR?=	M33lUFEvOiCvTR?=		NXnxPYF6TE2VTx?=	M3P4eYlvcGmkaYTzJJRp\SCHeIDy[ZN{cW:wIH;mJGFlcXCxZ3Xu[ZNqe1KnbHH0[YQhXHKjboPjdolxfGmxbjDGZYN1d3K\|IHHu[EBO[XKtZYLz	MorQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2OEOzNFQoRjF7NEizN\|A1RC:jPh?=
3T3-L1	MW\GeY5kfGmxbjDhd5NigQ>?	MWWxMlIhdU1?		MkDmSG1UVw>?	NVXFNnZ1cW6qaXLpeJMhVWm2b4TpZ{BEdG:wYXygSZhx[W6\|aX;u	MnTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2OEOzNFQoRjF7NEizN\|A1RC:jPh?=
3T3-L1	NYjKOpVn[3m2b4TvfIlkcXS7IHHzd4F6	NIjuVnYyNjJibV2=		Mm\ESG1UVw>?	NI\0XI5l\WO{ZXHz[ZMhS2WubDDWbYFjcWyrdIm=	MoS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2OEOzNFQoRjF7NEizN\|A1RC:jPh?=
3T3-L1	NGDCWoJMcW6jc3WgZZN{[Xl?	MX2xMlIhdU1?		MVvEUXNQ	MUjhZ5RqfmG2ZYOgRW1RUw>?	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
L6 skeletal muscle cells	MYXGeY5kfGmxbjDhd5NigQ>?	M3HnfVI2OCEQvF2=		M1jaUGROW09?	MkjsZYN1cX[jdHXzJGFOWEtic3nncoFtcW6pIIDheIh4[Xm\|	NYHES2ZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlg5OzZpPkG5PFI5QDN4PD;hQi=>
L6 skeletal muscle cells	NX\mN41kTnWwY4Tpc44h[XO\|YYm=	M3zPXFI2OCEQvF2=		NF23W4VFVVOR	MUfpcohq[mm2czD0bIUhVmFtLVurMWFVWGG\|ZTD0doFve3CxcoSgZYN1cX[rdImgZY5lKGOnbHygd5Vz\mGlZTDhZpVv\GGwY3W=	Mn7yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mki4N\|YoRjF7OEK4PFM3RC:jPh?=
MDA-MB231	MlHBRZBweHSxc3nzJIF{e2G7	MojzOFAxKM7:TR?=		M4rOVmROW09?	MWfz[Y5{cXSrenXzJIh2dWGwIHLy[YF{fCClYX7j[ZIh[2WubDDsbY5meyC2bzDUVmFKVC2rbnT1Z4VlKGGyb4D0c5Nqew>?	MojiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OU[0OlkoRjF7OEm2OFY6RC:jPh?=
BT474	MoLRRZBweHSxc3nzJIF{e2G7	NULIRlY3PDByIN88US=>		Mn;uSG1UVw>?	MVrz[Y5{cXSrenXzJIh2dWGwIHLy[YF{fCClYX7j[ZIh[2WubDDsbY5meyC2bzDUVmFKVC2rbnT1Z4VlKGGyb4D0c5Nqew>?	M3XkWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEm2OFY6Lz5zOUi5OlQ3QTxxYU6=
MCF7	NF\1NYhCeG:ydH;zbZMh[XO\|YYm=	MUm0NFAh\|ryP		MVnEUXNQ	NGXPbld{\W6\|aYTpfoV{KGi3bXHuJIJz\WG\|dDDjZY5k\XJiY3XscEBtcW6nczD0c{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orcx?=	NIPEbZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi5OlQ3QSd-MUm4PVY1Pjl:L3G+
Mesenchymal stem cells	M1ezNWtqdmG\|ZTDhd5NigQ>?	M4DXSVExKML3TR?=		NXrlZog4TE2VTx?=	NFGw[IFqdmS3Y3XzJIEhem:kdYP0JIFv\CC\|dYP0ZYlv\WRiQV3QT{Bi[3SrdnH0bY9v	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyNEi3PUc,OjRzMES4O\|k9N2F-
Mesenchymal stem cells	NYTjO5Vl[3m2b4TvfIlkcXS7IHHzd4F6	M3fLWlExOCEEtV2=		M2rMU2ROW09?	NF\ETnRl\WO{ZXHz[ZMhfGinIF3TR{Bxem:uaX\ldoF1cW:w	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyNEi3PUc,OjRzMES4O\|k9N2F-
MG-63	MU\jfZRwfG:6aXPpeJkh[XO\|YYm=	Ml36NVAhyrWP		NVPxRZp{TE2VTx?=	NW\VT5FKcW6qaXLpeJMhUDKRMj3JcoR2[2WmIF;zeIVw[myjc4SgR4VtdCCGZXH0bC=>	MlH4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN\|YzRC:jPh?=
MC3T3-E1	MYPjfZRwfG:6aXPpeJkh[XO\|YYm=	NF3vbGsyOCEEtV2=		NHLmeYdFVVOR	NH7VNZpqdmirYnn0d{BJOk9{LVnu[JVk\WRiT4P0[Y9jdGG\|dDDD[YxtKESnYYTo	Ml7RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN\|YzRC:jPh?=
MG-63	NGnlSGNCeG:ydH;zbZMh[XO\|YYm=	M3zSbVExKML3TR?=		NHXjeXlFVVOR	Mn\zd5VxeHKnc4Pld{BJOk9{LVnu[JVk\WRiT4P0[Y9jdGG\|dDDD[YxtKEGyb4D0c5Nqew>?	NE\vR5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFM3Oid-MkS5OlA{PjJ:L3G+
MC3T3-E1	NXH6VJN5SXCxcITvd4l{KGG\|c3H5	M4XROFExKML3TR?=		MlTNSG1UVw>?	Mmnod5VxeHKnc4Pld{BJOk9{LVnu[JVk\WRiT4P0[Y9jdGG\|dDDD[YxtKEGyb4D0c5Nqew>?	NE\m[5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFM3Oid-MkS5OlA{PjJ:L3G+
MG-63	MV7GeY5kfGmxbjDhd5NigQ>?	MYGxNEDDvU1?		MWLEUXNQ	MmHyZYxt\X[rYYTld{BTV1NiYXPjeY12dGG2aX;uJIFv\CCDVGCg[IVxdGW2aX;uJINifXOnZDDifUBJOk9{	Mnr1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN\|YzRC:jPh?=
MC3T3-E1	MUTGeY5kfGmxbjDhd5NigQ>?	Ml7LNVAhyrWP		NYfMW5o6TE2VTx?=	M4rkRoFtdGW4aXH0[ZMhWk:VIHHjZ5VufWyjdHnvckBidmRiQWTQJIRmeGyndHnvckBk[XW\|ZXSgZpkhUDKRMh?=	M3S5RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wN\|YzLz5{NEm2NFM3OjxxYU6=
MG-63	NWfkZ\|BNTnWwY4Tpc44h[XO\|YYm=	MYKxNEDDvU1?		NFX5ZmlFVVOR	NX;4O3p{\mGlaXzpeIF1\XNiSELPNk1qdmS3Y3XkJIF2fG:yaHHnfUBi[3SrdnH0bY9v	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4MEO2Nkc,OjR7NkCzOlI9N2F-
MC3T3-E1	M1;pXmZ2dmO2aX;uJIF{e2G7	NVf6ZW5pOTBiwsXN		Mk\QSG1UVw>?	NFfDVIln[WOrbHn0ZZRmeyCKMl:yMYlv\HWlZXSgZZV1d3CqYXf5JIFkfGm4YYTpc44>	M4foUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wN\|YzLz5{NEm2NFM3OjxxYU6=
PC3	NIT0bZRMcW6jc3WgZZN{[Xl?	MonsNVAxKML3TR?=		M3\BVGROW09?	MlWzeZBz\We3bHH0[ZMhfGinIHzleoVteyCxZjDBUXBMKGGwZDDBR2MheGixc4Doc5J6dGG2aX;u	NGXZWIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5OFA1Oyd-MkW1PVQxPDN:L3G+
PC3M	MVfLbY5ie2ViYYPzZZk>	M2nDe\|ExOCEEtV2=		MXnEUXNQ	NXvaNXIxfXC{ZXf1cIF1\XNidHjlJIxmfmWuczDv[kBCVVCNIHHu[EBCS0NicHjvd5Bpd3K7bHH0bY9v	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV7NEC0N{c,OjV3OUSwOFM9N2F-
PC3	MWrGeY5kfGmxbjDhd5NigQ>?	MmTwNVAxKML3TR?=		Ml25SG1UVw>?	NIPnSmdqdmS3Y3XzJHBKO0txbWTPVkBx[XSqd3H5dy=>	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV7NEC0N{c,OjV3OUSwOFM9N2F-
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HDLM-2	NInZVGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWXJcohq[mm2aX;uJI9nKGi3bXHuJGhFVE1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyIE2gNE4yOzZzNjFOwG0v	NUHuWXVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
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CAL-27	NFTUZlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2HBTmlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA1QS53M{O0JO69VS5?	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
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MCF7	NFTKXIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUTJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IES5MlU5PjVizszNMi=>	NIPJW4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTJ3MESvK\|5USU6JRWK8M4E,
T47D	NV\t[\|NLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkC3TY5pcWKrdHnvckBw\iCqdX3hckBVPDeGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTC0PU44ODB5IN88UU4>	NILBem09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTJ3MESvK\|5USU6JRWK8M4E,
YKG-1	NWPMe2dOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NUnafWFJUW6qaXLpeIlwdiCxZjDoeY1idiC\S1etNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hPDlwOUSxNkDPxE1w	M3zMdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin ; PubMed: 26431377 Oncotarget A & B. MCF-7 cells were treated with 100–400 μM of A-769662 for 4-24 hours. Protein was harvested and Western blot analysis was done to determine levels of pAMPK at threonine 172, pCofilin at serine 3, detyrosinated alpha tubulin (glu-tub), and acetylated alpha tubulin (acetyl-tub). C & D. BT-549 cells were treated with 100–400 μM A-769662 for 4–24 hours. Protein was harvested and Western blot analysis was done to determine levels of markers listed above. p-AMPK / AMPK / p-S6K / S6K / p-ACC ; PubMed: 22456226 Neurosignals Representative immunoblots for p-AMPK, total AMPK, p-S6K, total S6K, p-ACC and actin under control conditions (C) and in response to 50 μM A-769662 at various time points.	26431377 22456226

In vivo

Short-term treatment of normal Sprague Dawley rats with A-769662 decreases liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreases hepatic expression of PEPCK, G6Pase, and FAS, lowers plasma glucose by 40%, reduced body weight gain and significantly decreases both plasma and liver triglyceride levels. ^[1]

Protocol

Kinase Assay: ^[1]	- Collapse 96-well AMPK assay: AMPK activity is measured by monitoring phosphorylation of the SAMS peptide substrate (20 mM in standard assays and 100 mM in additivity assays) following a previously described protocol (Anderson et al., 2004). To determine whether A-769662-induced AMPK activation occurs in a reversible manner, AMP or A-769662 are preincubated with rat liver AMPK for 10 minutes at 20 times standard assay concentrations prior to dilution and measurement of AMPK activity.
Cell Research: ^[3]	- Collapse Cell lines: MEF cells Concentrations: 300 μM Incubation Time: 24 hours Method: Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Sprague Dawley rats Dosages: 30 mg/kg Administration: Administered via i.p. (Only for Reference)

References

[4] Pevton KJ, et al, J Pharmacol Exp Ther, 2012, Jun 13.

- Collapse

Solubility (25°C)

In vitro	DMSO	72 mg/mL (199.78 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O For best results, use promptly after mixing.	5 mg/ml

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download A-769662 SDF

Molecular Weight	360.39
Formula	C₂₀H₁₂N₂O₃S
CAS No.	844499-71-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	C1=CC=C(C(=C1)C2=CC=C(C=C2)C3=CSC4=C3C(=C(C(=O)N4)C#N)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

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The Serial Dilution Calculator Equation

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Molecular Weight Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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