A-769662

Name: A-769662
Brand: Selleck Chemicals
SKU: S2697
Rating: 5 (51 reviews)

For research use only.

Catalog No.S2697

51 publications

CAS No. 844499-71-4

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

Selleck's A-769662 has been cited by 51 publications

cell, 2019, 178(5):1102-1114

PubMed: 31442403 ( click the link to review the publication )

Cell Metab, 2019, 30(3):508-524

PubMed: 31204282 ( click the link to review the publication )

Cell Metab, 2019, 29(6):1350-1362

PubMed: 30982734 ( click the link to review the publication )

Mol Cell, 2021, S1097-2765(21)00232-X

PubMed: 33909988 ( click the link to review the publication )

Theranostics, 2021, 11(3):1079-1099

PubMed: 33391522 ( click the link to review the publication )

Cell Death Differ, 2021, 10.1038/s41418-021-00777-0

PubMed: 34040167 ( click the link to review the publication )

Cell Death Dis, 2021, 12(4):385

PubMed: 33837179 ( click the link to review the publication )

Mol Cancer Res, 2021, molcanres.0808.2020

PubMed: 33980612 ( click the link to review the publication )

Mol Cell, 2020, S1097-2765(20)30900-X

PubMed: 33400924 ( click the link to review the publication )

Mol Cell, 2020, S1097-2765(20)30786-3

PubMed: 33271062 ( click the link to review the publication )

FASEB J, 2020, 34(3):4702-4717

PubMed: 32030825 ( click the link to review the publication )

J Lipid Res, 2020, 10.1194/jlr.RA119000542

PubMed: 31964763 ( click the link to review the publication )

Front Oncol, 2020, 10:956

PubMed: 32596161 ( click the link to review the publication )

Am J Physiol Cell Physiol, 2020, 1;318(5):C1018-C1029

PubMed: 32293932 ( click the link to review the publication )

Cell Biosci, 2020, 10:79

PubMed: 32549974 ( click the link to review the publication )

Oxid Med Cell Longev, 2020, 2020:8708236

PubMed: 32104542 ( click the link to review the publication )

Cell Res, 2019, 29(6):460-473

PubMed: 30948787 ( click the link to review the publication )

Mol Cell, 2019, 10.1016/j.molcel.2019.09.007

PubMed: 31586547 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):192

PubMed: 31088566 ( click the link to review the publication )

Sci Rep, 2019, 9(1):1897

PubMed: 30760778 ( click the link to review the publication )

Cell Signal, 2019, 57:45-57

PubMed: 30772465 ( click the link to review the publication )

Am J Physiol Renal Physiol, 2019, 317(6):F1513-F1525

PubMed: 31566435 ( click the link to review the publication )

BMC Complement Altern Med, 2019, 19(1):255

PubMed: 31519174 ( click the link to review the publication )

Biol Pharm Bull, 2019, 42(8):1303-1309

PubMed: 31366866 ( click the link to review the publication )
Cell Rep, 2019, 29(6):1524-1538

PubMed: 31693893 ( click the link to review the publication )

Mol Cell, 2018, 69(2):279-291

PubMed: 29351847 ( click the link to review the publication )

Cancer Sci, 2018, 109(5):1468-1479

PubMed: 29516572 ( click the link to review the publication )

Food Chem Toxicol, 2018, 122:143-150

PubMed: 30316840 ( click the link to review the publication )

Front Mol Neurosci, 2018, 11:98

PubMed: 29666569 ( click the link to review the publication )

Integr Cancer Ther, 2018, 17(2):511-523

PubMed: 28627240 ( click the link to review the publication )

Nat Cell Biol, 2017, 19(6):724-731

PubMed: 28553939 ( click the link to review the publication )

J Crohns Colitis, 2017, 11(12):1480-1490

PubMed: 28961920 ( click the link to review the publication )

J Exp Clin Cancer Res, 2017, 36(1):190

PubMed: 29273065 ( click the link to review the publication )

Sci Rep, 2017, 7(1):12891

PubMed: 29018223 ( click the link to review the publication )

Sci Rep, 2017, 7(1):16373

PubMed: 29180765 ( click the link to review the publication )

Biochem Biophys Res Commun, 2017, 492(3):520-527

PubMed: 28807827 ( click the link to review the publication )

Mol Med Rep, 2017, 16(3):3648-3656

PubMed: 28765969 ( click the link to review the publication )

Autophagy, 2016, 12(2):432-8

PubMed: 26902588 ( click the link to review the publication )

Sci Rep, 2016, 6:36721

PubMed: 27830724 ( click the link to review the publication )

Am J Physiol Endocrinol Metab, 2016, 311(4):E706-E719

PubMed: 27577855 ( click the link to review the publication )

Cell Rep, 2015, 12(4):599-609

PubMed: 26190112 ( click the link to review the publication )

Apoptosis, 2015, 20(10):1373-87

PubMed: 26276035 ( click the link to review the publication )

Cell Signal, 2015, 27(5):978-88

PubMed: 25683918 ( click the link to review the publication )

Cell Signal, 2015, 27(5):978-88

PubMed: ( click the link to review the publication )

Can J Physiol Pharmacol, 2015, 93(7):585-95

PubMed: 26120822 ( click the link to review the publication )

Cancer Res, 2014, 74(1):298-308

PubMed: 24240701 ( click the link to review the publication )

Pharmacol Res, 2014, 81:34-43

PubMed: 24508566 ( click the link to review the publication )

J Lipid Res, 2014, 55(7):1254-1266

PubMed: 24864274 ( click the link to review the publication )
Lung Cancer, 2014, 86(2):151-7

PubMed: 25240516 ( click the link to review the publication )

Am J Physiol Endocrinol Metab, 2014, 306(6):E688-96

PubMed: 24425763 ( click the link to review the publication )

Biochem Biophys Res Commun, 2013, 437(1):1-6

PubMed: 23743198 ( click the link to review the publication )

Purity & Quality Control

Choose Selective AMPK Inhibitors

Biological Activity

Description

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

Targets

AMPK ^[1] (Cell-free assay)	Fatty acid synthesis ^[1] (in primary rat hepatocytes)
0.8 μM(EC50)	3.2 μM

In vitro

A-769662 stimulates partially purified rat liver AMPK with EC50 with 0.8 μM. A-769662 activates AMPK purified from multiple tissues and species in a dose-responsive manner with modest variations in observed EC50s. EC50s determined for A-769662 using partially purified AMPK extracts from rat heart, rat muscle, or human embryonic kidney cells (HEKs) are 2.2 mM, 1.9 mM, or 1.1 mM, respectively. A 4 hours treatment of primary rat hepatocytes with A-769662 dose-dependently increases ACC phosphorylation, which correlated inhibition of fatty acid synthesis with IC50 of 3.2 μM. A-769662 also inhibits fatty acid sythesis in mouse hepatocytes with IC50 with 3.6 μM ^[1] A-769662 activates AMPK both allosterically and by inhibiting dephosphorylation of AMPK on Thr-172, similar to the effects of AMP. ^[2] A-769662 inhibits proteasomal function by an AMPK-independent mechanism. A-769662 affects the in vitro activity of purified 26S proteasomes but not the in vitro activity of purified 20S proteasomes. A-769662 has toxic effects on MEF cells. ^[3] A recent research shows A-769662 inhibited cell proliferation and DNA synthesis. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
mouse hepatocytes	M2TqN2Z2dmO2aX;uJIF{e2G7	NXfNSm1LOSCvTR?=		NETLNFVFVVOR	M3jQbIlvcGmkaYTzJIZifHS7IHHjbYQhe3mwdHjld4l{KHerdHigTWM2OCCxZjCzMlYh\|ryP	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjd3M{W3Okc,OTZ5NUO1O\|Y9N2F-
rat hepatocytes	MmjXSpVv[3Srb36gZZN{[Xl?	MVOxJI1O		NWHXWHB2TE2VTx?=	NFK0R4ZqdmirYnn0d{Bn[XS2eTDhZ4llKHO7boTo[ZNqeyC5aYToJGlEPTBib3[gN{43KM7:TR?=	MlnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5NUO1O\|YoRjF4N{WzOVc3RC:jPh?=
HEK293	MlTZT4lv[XOnIHHzd4F6	MWCyNFAh\|ryP		M3PDcWROW09?	NYrTcoJZ[WO2aY\heIV{KGWwZH;n[Y5wfXNiQV3QTy=>	NUTFZ4V6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NlgzPDFpPkG3O\|I5OjRzPD;hQi=>
CCL13	M1LzTGtqdmG\|ZTDhd5NigQ>?	NUThNY5yOjByIN88US=>		MnvUSG1UVw>?	M3\DS4FkfGm4YYTld{BmdmSxZ3Xuc5V{KEGPUFu=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd{OEK0NUc,OTd5MkiyOFE9N2F-
MEFs	MWnGeY5kfGmxbjDhd5NigQ>?	NUHpPWJ4OzByIN88US=>		M4rzeGROW09?	NF[2VXVqdmirYnn0d{Bxem:2ZXHzc41idCCodX7jeIlwdiCkeTDhckBCVVCNLXnu[IVx\W6mZX70JI1m[2ijbnnzcS=>	M4fWWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUmzOVg1Lz5zOEW5N\|U5PDxxYU6=
epididymal clear cells	MX;GeY5kfGmxbjDhd5NigQ>?	M4rPZ\|IxOCEQvF2=		MmW1SG1UVw>?	NWX5SVJRcW6qaXLpeJMhfGinIIDIMY1m\GmjdHXkJHYuSVSSYYPlJIFk[3WvdXzheIlwdiCjdDD0bIUh[XCrY3HsJI1mdWK{YX7l	Mn34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{MUG5NVgoRjF7MkGxPVE5RC:jPh?=
3T3-L1	M{LmcmZ2dmO2aX;uJIF{e2G7	M3LTRVEvOiCvTR?=		MoWySG1UVw>?	M1nTV4lvcGmkaYTzJFNVOy2OMTDB[Ilxd2enbnXzbZM>	NWrEd2VlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0PFM{ODRpPkG5OFg{OzB2PD;hQi=>
3T3-L1	MVXGeY5kfGmxbjDhd5NigQ>?	M4DYOlEvOiCvTR?=		MXTEUXNQ	NFnZVZFqdmirYnn0d{B1cGViRYjwdoV{e2mxbjDv[kBC\Gmyb3flcoV{cXOUZXzheIVlKFS{YX7zZ5JqeHSrb36gSoFkfG:{czDhcoQhVWG{a3Xydy=>	NVfrXpprRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0PFM{ODRpPkG5OFg{OzB2PD;hQi=>
3T3-L1	M{HW[mZ2dmO2aX;uJIF{e2G7	MWexMlIhdU1?		M3LSW2ROW09?	NWr4Vmt1cW6qaXLpeJMhVWm2b4TpZ{BEdG:wYXygSZhx[W6\|aX;u	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
3T3-L1	NVPFeFZp[3m2b4TvfIlkcXS7IHHzd4F6	NFu2NI0yNjJibV2=		M1LZfWROW09?	M1PEeYRm[3KnYYPld{BE\WyuIG\pZYJqdGm2eR?=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
3T3-L1	MlnMT4lv[XOnIHHzd4F6	M4r2UFEvOiCvTR?=		NYjFbG5GTE2VTx?=	MkjpZYN1cX[jdHXzJGFOWEt?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
L6 skeletal muscle cells	MWHGeY5kfGmxbjDhd5NigQ>?	NVTNflYzOjVyIN88US=>		MYrEUXNQ	MVLhZ5RqfmG2ZYOgRW1RUyC\|aXfuZYxqdmdicHH0bJdigXN?	MkiwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mki4N\|YoRjF7OEK4PFM3RC:jPh?=
L6 skeletal muscle cells	MYTGeY5kfGmxbjDhd5NigQ>?	MlPoNlUxKM7:TR?=		M{TmOWROW09?	NVTXbHJxcW6qaXLpeJMhfGinIF7hL{1MMy2DVGDhd4UhfHKjboPwc5J1KGGldHn2bZR6KGGwZDDj[YxtKHO3cn\hZ4Uh[WK3bnThcoNm	MkTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6Mki4N\|YoRjF7OEK4PFM3RC:jPh?=
MDA-MB231	M3zaU2Fxd3C2b4Ppd{Bie3OjeR?=	MWC0NFAh\|ryP		NVnXWpNiTE2VTx?=	MlHXd4Vve2m2aYrld{BpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHygcIlv\XNidH:gWHJCUUxvaX7keYNm\CCjcH;weI9{cXN?	MlvHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OU[0OlkoRjF7OEm2OFY6RC:jPh?=
BT474	Mn\PRZBweHSxc3nzJIF{e2G7	NYj6OoU4PDByIN88US=>		MYXEUXNQ	NU\Zdopze2Wwc3n0bZpmeyCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hXFKDSVytbY5lfWOnZDDhdI9xfG:\|aYO=	MnHFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OU[0OlkoRjF7OEm2OFY6RC:jPh?=
MCF7	M3zJNWFxd3C2b4Ppd{Bie3OjeR?=	NIqyNZA1ODBizszN		NVzqWpFpTE2VTx?=	NH61V5F{\W6\|aYTpfoV{KGi3bXHuJIJz\WG\|dDDjZY5k\XJiY3XscEBtcW6nczD0c{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orcx?=	NELMNpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi5OlQ3QSd-MUm4PVY1Pjl:L3G+
Mesenchymal stem cells	NV;ocFNLU2mwYYPlJIF{e2G7	MXOxNEDDvU1?		Mkn4SG1UVw>?	NYnUVIRvcW6mdXPld{BiKHKxYoXzeEBidmRic4XzeIFqdmWmIFHNVGsh[WO2aY\heIlwdg>?	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyNEi3PUc,OjRzMES4O\|k9N2F-
Mesenchymal stem cells	NIH1[XdkgXSxdH;4bYNqfHliYYPzZZk>	M2Dp[lExOCEEtV2=		MlTTSG1UVw>?	MUXk[YNz\WG\|ZYOgeIhmKE2VQzDwdo9tcW[ncnH0bY9v	NFLwR\|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwOFg4QSd-MkSxNFQ5Pzl:L3G+
MG-63	NX3YOJc3[3m2b4TvfIlkcXS7IHHzd4F6	M1LRS\|ExKML3TR?=		M36xcGROW09?	NXzWU4hucW6qaXLpeJMhUDKRMj3JcoR2[2WmIF;zeIVw[myjc4SgR4VtdCCGZXH0bC=>	NFXLRXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFM3Oid-MkS5OlA{PjJ:L3G+
MC3T3-E1	M2HnW4N6fG:2b4jpZ4l1gSCjc4PhfS=>	M4fUbVExKML3TR?=		MmeySG1UVw>?	MnT2bY5pcWKrdIOgTFJQOi2LbnT1Z4VlKE:\|dHXvZoxie3RiQ3XscEBF\WG2aB?=	NUnyOoFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA{PjJpPkK0PVYxOzZ{PD;hQi=>
MG-63	NUPBRWx3SXCxcITvd4l{KGG\|c3H5	MnXWNVAhyrWP		M2fTTWROW09?	NXy0WG9we3WycILld5NmeyCKMl:yMWlv\HWlZXSgU5N1\W:kbHHzeEBE\WyuIFHwc5B1d3Orcx?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4MEO2Nkc,OjR7NkCzOlI9N2F-
MC3T3-E1	MoH1RZBweHSxc3nzJIF{e2G7	M2LjdVExKML3TR?=		M2\MT2ROW09?	M{jGN5N2eHC{ZYPz[ZMhUDKRMj3JcoR2[2WmIF;zeIVw[myjc4SgR4VtdCCDcH;weI9{cXN?	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4MEO2Nkc,OjR7NkCzOlI9N2F-
MG-63	M1K0Z2Z2dmO2aX;uJIF{e2G7	MUmxNEDDvU1?		NID0NFFFVVOR	MoqzZYxt\X[rYYTld{BTV1NiYXPjeY12dGG2aX;uJIFv\CCDVGCg[IVxdGW2aX;uJINifXOnZDDifUBJOk9{	NH3leFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFM3Oid-MkS5OlA{PjJ:L3G+
MC3T3-E1	M{\OZWZ2dmO2aX;uJIF{e2G7	MWWxNEDDvU1?		NEf5cnFFVVOR	Mn\1ZYxt\X[rYYTld{BTV1NiYXPjeY12dGG2aX;uJIFv\CCDVGCg[IVxdGW2aX;uJINifXOnZDDifUBJOk9{	MojXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN\|YzRC:jPh?=
MG-63	Ml:5SpVv[3Srb36gZZN{[Xl?	MXmxNEDDvU1?		Mlr2SG1UVw>?	MYnmZYNqdGm2YYTld{BJOk9{LXnu[JVk\WRiYYX0c5Bp[We7IHHjeIl3[XSrb36=	NXrGPIU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA{PjJpPkK0PVYxOzZ{PD;hQi=>
MC3T3-E1	MlPkSpVv[3Srb36gZZN{[Xl?	M3OyWlExKML3TR?=		NIPsOFBFVVOR	Mlny[oFkcWyrdHH0[ZMhUDKRMj3pcoR2[2WmIHH1eI9xcGGpeTDhZ5RqfmG2aX;u	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4MEO2Nkc,OjR7NkCzOlI9N2F-
PC3	NWXzZYVCU2mwYYPlJIF{e2G7	NH;DUHUyODBiwsXN		MkDXSG1UVw>?	M3rnTJVxemWpdXzheIV{KHSqZTDs[ZZmdHNib3[gRW1RUyCjbnSgRWNEKHCqb4PwbI9zgWyjdHnvci=>	MnrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUSwOFMoRjJ3NUm0NFQ{RC:jPh?=
PC3M	NEHZeolMcW6jc3WgZZN{[Xl?	MXqxNFAhyrWP		NU\BXnR1TE2VTx?=	NWnBXpAxfXC{ZXf1cIF1\XNidHjlJIxmfmWuczDv[kBCVVCNIHHu[EBCS0NicHjvd5Bpd3K7bHH0bY9v	M3roeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUm0NFQ{Lz5{NUW5OFA1OzxxYU6=
PC3	MkjhSpVv[3Srb36gZZN{[Xl?	MnyxNVAxKML3TR?=		MUXEUXNQ	Ml7UbY5lfWOnczDQTVNMN22WT2KgdIF1cHejeYO=	M2nsNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUm0NFQ{Lz5{NUW5OFA1OzxxYU6=
PC3M	M2jHfmZ2dmO2aX;uJIF{e2G7	MmPJNVAxKML3TR?=		M3PZTmROW09?	MkixbY5lfWOnczDQTVNMN22WT2KgdIF1cHejeYO=	MnrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUSwOFMoRjJ3NUm0NFQ{RC:jPh?=
PC3	NIHlfWdIem:5dHigbY5pcWKrdH;yfUBie3OjeR?=	NInZUWgyODBiwsXN		MUXEUXNQ	NID3Z5F{fXCycnXzd4V{KHC{b3zp[oVz[XSrb36=	NWP6V2dDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVQxPDNpPkK1OVk1ODR\|PD;hQi=>
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BC-1	NYTPe5hjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWD2PYV7UW6qaXLpeIlwdiCxZjDoeY1idiCEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjB4OUezJO69VS5?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
CTV-1	MkDDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYjJcohq[mm2aX;uJI9nKGi3bXHuJGNVXi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlA4OzB\|IN88UU4>	NW\kN\|U1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
HC-1	M3z2fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGhENTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNFg{OTRizszNMi=>	M4rPRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
JiyoyeP-2003	MmjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1L6TmlvcGmkaYTpc44hd2ZiaIXtZY4hUmm7b4nlVE0zODB\|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlA5OzdzIN88UU4>	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
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EoL-1-cell	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NY\SU5V2UW6qaXLpeIlwdiCxZjDoeY1idiCHb1ytNU1k\WyuIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlA6PTV6IN88UU4>	M1zzPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
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HL-60	NG\2OmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUHJcohq[mm2aX;uJI9nKGi3bXHuJGhNNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlA6PzN7IN88UU4>	M3jV[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
MC116	M1fjVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MUnJcohq[mm2aX;uJI9nKGi3bXHuJG1EOTF4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlExODF2IN88UU4>	MnzMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
CESS	Mn36S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2rhdWlvcGmkaYTpc44hd2ZiaIXtZY4hS0WVUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyIE2gNE4yODFzIN88UU4>	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
REH	M3HpV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2XBOWlvcGmkaYTpc44hd2ZiaIXtZY4hWkWKIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlExOzh{IN88UU4>	M2PxS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
HCC2157	NX\iUnBuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MYLJcohq[mm2aX;uJI9nKGi3bXHuJGhESzJzNUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvOTB2NUWg{txONg>?	MnSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
HEL	NX:zNZlQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkLOTY5pcWKrdHnvckBw\iCqdX3hckBJTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNVA4OjhizszNMi=>	Mk\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
BL-70	M1rZXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Ml\JTY5pcWKrdHnvckBw\iCqdX3hckBDVC15MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyIE2gNE4yODhyNTFOwG0v	NV;rcoE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
NCI-H1770	NWPvco9QT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3XtRWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixO\|cxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkGwPFY2KM7:TT6=	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
SK-MM-2	NH76W4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUPRVFFOUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NUU0zKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkGwPVQ1KM7:TT6=	MkPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
MONO-MAC-6	M4Xhdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmDCTY5pcWKrdHnvckBw\iCqdX3hckBOV06RLV3BR{03KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkGxN\|czKM7:TT6=	NVr1SXZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
SR	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3HER2lvcGmkaYTpc44hd2ZiaIXtZY4hW1JiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuNVE{QDdizszNMi=>	M4HUblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
MOLT-4	NGO1dlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWLJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyIE2gNE4yOTR2NjFOwG0v	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
KE-37	M3XueWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGLIbHNKdmirYnn0bY9vKG:oIHj1cYFvKEuHLUO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjF{OUK0JO69VS5?	NWr4WopVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
ST486	NX71T5psT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2DicmlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvOTN3Mkeg{txONg>?	Mo\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
HDLM-2	NHToZZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NHywWJRKdmirYnn0bY9vKG:oIHj1cYFvKEiGTF2tNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC5zM{[xOkDPxE1w	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
ARH-77	NGXMPVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkLKTY5pcWKrdHnvckBw\iCqdX3hckBCWkhvN{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvOTRyNjFOwG0v	MkPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
EB2	NV;ZbYVZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1r3TWlvcGmkaYTpc44hd2ZiaIXtZY4hTUJ{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTBiPTCwMlE1OjN2IN88UU4>	Mn7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
CCRF-CEM	NH3uNnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnryTY5pcWKrdHnvckBw\iCqdX3hckBES1KILVPFUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC5zNEW3OkDPxE1w	M{\BflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
HD-MY-Z	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnzsTY5pcWKrdHnvckBw\iCqdX3hckBJTC2PWT3aJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjF2N{mxJO69VS5?	NF7TZ2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTJ3MESvK\|5USU6JRWK8M4E,
BT-474	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NInSUoVKdmirYnn0bY9vKG:oIHj1cYFvKEKWLUS3OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC5zNUC3OEDPxE1w	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+
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... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin ; PubMed: 26431377 Oncotarget A & B. MCF-7 cells were treated with 100–400 μM of A-769662 for 4-24 hours. Protein was harvested and Western blot analysis was done to determine levels of pAMPK at threonine 172, pCofilin at serine 3, detyrosinated alpha tubulin (glu-tub), and acetylated alpha tubulin (acetyl-tub). C & D. BT-549 cells were treated with 100–400 μM A-769662 for 4–24 hours. Protein was harvested and Western blot analysis was done to determine levels of markers listed above. p-AMPK / AMPK / p-S6K / S6K / p-ACC ; PubMed: 22456226 Neurosignals Representative immunoblots for p-AMPK, total AMPK, p-S6K, total S6K, p-ACC and actin under control conditions (C) and in response to 50 μM A-769662 at various time points.	26431377 22456226

In vivo

Short-term treatment of normal Sprague Dawley rats with A-769662 decreases liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreases hepatic expression of PEPCK, G6Pase, and FAS, lowers plasma glucose by 40%, reduced body weight gain and significantly decreases both plasma and liver triglyceride levels. ^[1]

Protocol

Kinase Assay: ^[1]	- Collapse 96-well AMPK assay: AMPK activity is measured by monitoring phosphorylation of the SAMS peptide substrate (20 mM in standard assays and 100 mM in additivity assays) following a previously described protocol (Anderson et al., 2004). To determine whether A-769662-induced AMPK activation occurs in a reversible manner, AMP or A-769662 are preincubated with rat liver AMPK for 10 minutes at 20 times standard assay concentrations prior to dilution and measurement of AMPK activity.
Cell Research: ^[3]	- Collapse Cell lines: MEF cells Concentrations: 300 μM Incubation Time: 24 hours Method: Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Sprague Dawley rats Dosages: 30 mg/kg Administration: Administered via i.p. (Only for Reference)

References

[4] Pevton KJ, et al, J Pharmacol Exp Ther, 2012, Jun 13.

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Solubility (25°C)

In vitro	DMSO	72 mg/mL (199.78 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O For best results, use promptly after mixing.	5 mg/ml

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download A-769662 SDF

Molecular Weight	360.39
Formula	C₂₀H₁₂N₂O₃S
CAS No.	844499-71-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	C1=CC=C(C(=C1)C2=CC=C(C=C2)C3=CSC4=C3C(=C(C(=O)N4)C#N)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

Serial Dilutions
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Computed Result

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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A-769662