Home PI3K/Akt/mTOR AMPK activator ex229 (compound 991) For research use only.

ex229 (compound 991)

EX229 (compound 991) is a potent AMPK activator that is 5-10-fold more potent than A769662 in activating AMPK.

ex229 (compound 991) Chemical Structure

ex229 (compound 991) Chemical Structure

CAS: 1219739-36-2

Selleck's ex229 (compound 991) has been cited by 8 publications

Purity & Quality Control

Batch: S865401 DMSO] 12 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 98.83%
98.83

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf21 Function assay 30 mins Agonist activity at human recombinant phosphorylated AMPK complex 7 alpha2/beta1/gamma1 expressed in baculovirus infected Sf21 cells assessed as phosphorylation of 5-FAM-labeled SAMS substrate preincubated for 30 mins in presence of AMPK activator followe, EC50=0.002μM 29035567
Sf21 Function assay 30 mins Activation of full length human recombinant AMPK alpha1/beta1/gamma1 expressed in baculovirus infected sf21 cells using SAMS peptide substrate after 30 mins in presence of [33P]ATP by TopCount analysis, EC50=0.003μM 27727125
Click to View More Cell Line Experimental Data

Biological Activity

Description EX229 (compound 991) is a potent AMPK activator that is 5-10-fold more potent than A769662 in activating AMPK.
Targets
AMPK [1]
In vitro
In vitro

EX229 activates AMPK in incubated rat epitrochlearis skeletal muscle and incubation of rat skeletal muscle with ex229 increases glucose uptake. The stimulation of skeletal muscle glucose uptake by ex229 is PI3K/PKB-independent, while AMPK-dependent. ex229 increases fatty acid oxidation and glucose uptake in L6 myotubes[1].
Cell Research Cell lines HEK293T cells
Concentrations 50 μM
Incubation Time 1 h
Method

Cells transfected with Myc-Rabep1 WT or Ser407Ala were treated for 60 min with vehicle or 50 μM 991.

Chemical Information & Solubility

Molecular Weight 431.87 Formula

C24H18ClN3O3
CAS No. 1219739-36-2 SDF --
Smiles CC1=C(C=C(C=C1)OC2=NC3=C(N2)C=C(C(=C3)C4=CC5=C(C=C4)N(C=C5)C)Cl)C(=O)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 12 mg/mL ( (27.78 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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