Gemcitabine HCl

Name: Gemcitabine HCl
Brand: Selleck Chemicals
SKU: S1149
Rating: 5 (90 reviews)

For research use only.

Catalog No.S1149 Synonyms: LY188011

90 publications

CAS No. 122111-03-9

Gemcitabine HCl (LY188011) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Selleck's Gemcitabine HCl has been cited by 90 publications

Cancer Cell, 2019, 35(3):519-533

PubMed: 30889383 ( click the link to review the publication )

Nat Commun, 2020, 11(1):3018

PubMed: 32541668 ( click the link to review the publication )

Nat Commun, 2020, 24;11(1):1975

PubMed: 32332851 ( click the link to review the publication )

Nat Commun, 2020, 10;11(1):2935

PubMed: 32523045 ( click the link to review the publication )

Nat Commun, 2020, 12;11(1):2971

PubMed: 32532990 ( click the link to review the publication )

Nat Commun, 2020, 14;11(1):1825

PubMed: 32286280 ( click the link to review the publication )

Clin Cancer Res, 2020, 27 pii: clincanres

PubMed: 32341033 ( click the link to review the publication )

Mol Syst Biol, 2020, 16(2):e8664

PubMed: 32073727 ( click the link to review the publication )

Oncogene, 2020, 10.1038/s41388-020-1364-7

PubMed: 32561852 ( click the link to review the publication )

Cancers (Basel), 2020, 12(12)E3816

PubMed: 33348809 ( click the link to review the publication )

Cancers (Basel), 2020, 15;12(4) pii: E974

PubMed: 32326402 ( click the link to review the publication )

Neoplasia, 2020, 22(1):22-32

PubMed: 31765939 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(10):3302-3315

PubMed: 33163271 ( click the link to review the publication )

Int J Mol Sci, 2020, 21(23)E9034

PubMed: 33261142 ( click the link to review the publication )

Sci Rep, 2020, 15;10(1):337

PubMed: 31941932 ( click the link to review the publication )

Sci Rep, 2020, 10(1):18489

PubMed: 33116269 ( click the link to review the publication )

J Biol Chem, 2020, 1 pii: jbc

PubMed: 32358063 ( click the link to review the publication )

Food Chem Toxicol, 2020, 147:111919

PubMed: 33301843 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):984

PubMed: 33046037 ( click the link to review the publication )

Cancer Res Treat, 2020, 10.4143/crt.2020.585

PubMed: 33070556 ( click the link to review the publication )

Med Sci Monit, 2020, 26:e922703

PubMed: 32946432 ( click the link to review the publication )

Anticancer Res, 2020, 40(7):3659-3667

PubMed: 32620605 ( click the link to review the publication )

Mediators Inflamm, 2020, 2020:8567320

PubMed: 32684837 ( click the link to review the publication )

bioRxiv, 2020, 2020/9/20.4.7.30734

PubMed: 32511305 ( click the link to review the publication )
Genes Chromosomes Cancer, 2020, 10.1002/gcc.22884

PubMed: 32614991 ( click the link to review the publication )

Am J Transl Res, 2020, 12(6):2827-2842

PubMed: 32655813 ( click the link to review the publication )

J Biochem Mol Toxicol, 2020, e22621

PubMed: 32894642 ( click the link to review the publication )

J Immunother Cancer, 2019, 7(1):199

PubMed: 31362778 ( click the link to review the publication )

J Control Release, 2019, 305:89-100

PubMed: 31096017 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E1835

PubMed: 31766357 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E2042

PubMed: 31861153 ( click the link to review the publication )

Cells, 2019, 8(8)

PubMed: 31390735 ( click the link to review the publication )

Cell Prolif, 2019, 52(1):e12514

PubMed: 30341797 ( click the link to review the publication )

Cancer Sci, 2019, 110(1):334-344

PubMed: 30353606 ( click the link to review the publication )

Int J Biol Sci, 2019, 15(9):1771-1786

PubMed: 31523182 ( click the link to review the publication )

Invest New Drugs, 2019, 10.1007/s10637-019-00839-8

PubMed: 31463638 ( click the link to review the publication )

BMC Cancer, 2019, 19(1):604

PubMed: 31216997 ( click the link to review the publication )

Biosci Rep, 2019, 10.1042/BSR20192358

PubMed: 31693728 ( click the link to review the publication )

Exp Ther Med, 2019, 18(1):550-558

PubMed: 31258693 ( click the link to review the publication )

Nanomedicine (Lond), 2019, 14(15):1971-1989

PubMed: 31355712 ( click the link to review the publication )

Nat Commun, 2018, 13;9(1):2720

PubMed: 30006524 ( click the link to review the publication )

Nat Commun, 2018, 9(1):2434

PubMed: 29934552 ( click the link to review the publication )

Sci Signal, 2018, 11(540)eaat0229

PubMed: 30042127 ( click the link to review the publication )

Cancers (Basel), 2018, 10(5)

PubMed: 29783721 ( click the link to review the publication )

Mol Ther Nucleic Acids, 2018, 13:472-482

PubMed: 30388621 ( click the link to review the publication )

Cell Chem Biol, 2018, 25(6):705-717

PubMed: 29628435 ( click the link to review the publication )

J Adv Res, 2018, 15:59-68

PubMed: 30581613 ( click the link to review the publication )

Neoplasia, 2018, 20(8):826-837

PubMed: 30015158 ( click the link to review the publication )
Oxid Med Cell Longev, 2018, 2018:2360427

PubMed: 29849873 ( click the link to review the publication )

Oxid Med Cell Longev, 2018, 2018:9482018

PubMed: 29765509 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(2)

PubMed: 29462944 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(10)

PubMed: 30274301 ( click the link to review the publication )

Apoptosis, 2018, 23(5-6):343-355

PubMed: 29740790 ( click the link to review the publication )

Pharm Res, 2018, 35(8):154

PubMed: 29855807 ( click the link to review the publication )

Dig Dis Sci, 2018, 63(12):3367-3375

PubMed: 30155839 ( click the link to review the publication )

Cancer Biomark, 2018, 10.3233/CBM-181182

PubMed: 29526843 ( click the link to review the publication )

Anticancer Res, 2018, 38(4):1967-1977

PubMed: 29599312 ( click the link to review the publication )

Oncol Lett, 2018, 15(5):6107-6114

PubMed: 29731842 ( click the link to review the publication )

Cancer Lett, 2017, 411:106-116

PubMed: 28947137 ( click the link to review the publication )

Cell Death Dis, 2017, 8(5):e2770

PubMed: 28492560 ( click the link to review the publication )

Br J Cancer, 2017, 116(3):324-334

PubMed: 28056464 ( click the link to review the publication )

Mol Oncol, 2017, 11(9):1130-1142

PubMed: 28544747 ( click the link to review the publication )

FEBS J, 2017, 284(2):222-236

PubMed: 27860276 ( click the link to review the publication )

J Cell Mol Med, 2017, 21(9):2055-2067

PubMed: 28244691 ( click the link to review the publication )

PLoS One, 2017, 12(11):e0188859

PubMed: 29186204 ( click the link to review the publication )

Acta Biochim Biophys Sin (Shanghai), 2017, 49(7):573-580

PubMed: 28475672 ( click the link to review the publication )

Oncotarget, 2017, 8(35):58417-58429

PubMed: 28938567 ( click the link to review the publication )

J Pineal Res, 2016, 60(1):27-38

PubMed: 26445000 ( click the link to review the publication )

Cancer Lett, 2016, 380(2):434-41

PubMed: 27400681 ( click the link to review the publication )

Antiviral Res, 2016, 126:69-80

PubMed: 26738783 ( click the link to review the publication )

Am J Pathol, 2016, 186(6):1537-46

PubMed: 27102771 ( click the link to review the publication )

PLoS One, 2016, 11(6):e0158617

PubMed: 27359113 ( click the link to review the publication )
Tumour Biol, 2016, 37(9):12731-12742

PubMed: 27448305 ( click the link to review the publication )

Sci Transl Med, 2015, 7(284):284ra57

PubMed: 25904741 ( click the link to review the publication )

Mol Cancer Ther, 2015,

PubMed: 25344582 ( click the link to review the publication )

Cancer Sci, 2015, 106(1):9-17

PubMed: 25363339 ( click the link to review the publication )

Biomedicine & Pharmacotherapy, 2015, 73:123-8

PubMed: 26211592 ( click the link to review the publication )

Eur J Pharmacol, 2015, 10.1016/j.ejphar.2015.03.064

PubMed: 25843411 ( click the link to review the publication )

PLoS One, 2015, 10(4):e0123901

PubMed: 25875914 ( click the link to review the publication )

Anticancer Drugs, 2015, 26(6):612-9

PubMed: 25734833 ( click the link to review the publication )

Nucleic Acids Res, 2014, 42(10):6436-47

PubMed: 24728989 ( click the link to review the publication )

Clin Cancer Res, 2014, 20(12):3187-97

PubMed: 24838526 ( click the link to review the publication )

J Immunol, 2014, 192(9):4273-83

PubMed: 24670807 ( click the link to review the publication )

Mol Pharmacol, 2014, 85(3):408-19

PubMed: 24353313 ( click the link to review the publication )

Pancreas, 2014, 44(1):144-151

PubMed: 25237909 ( click the link to review the publication )

Freien Universit, 2014, Christian K?hler

PubMed: ( click the link to review the publication )

Cell Death Dis, 2013, 4:e742

PubMed: 23887633 ( click the link to review the publication )

Cancer Immunol Immun, 2013, 62(2):383-91

PubMed: 22926062 ( click the link to review the publication )

Sci Signal, 2011, 1(4):352-65

PubMed: 22145099 ( click the link to review the publication )

Pancreas, 2011, 40(5):730-9

PubMed: 21633318 ( click the link to review the publication )

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description

Gemcitabine HCl (LY188011) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Features

Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.

Targets

DNA synthesis (Capan2 cells) ^[1]	DNA synthesis (BxPC3 cells) ^[1]	DNA synthesis (MIAPaCa2 cells) ^[1]	DNA synthesis (PANC1 cells) ^[1]
12 nM	18 nM	40 nM	50 nM

In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
CCRF-CEM	M{\RbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M3X1bmlEPTB;Mj65JOKyKDFwODDuUS=>	NUX1c\|NQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM/dCK−/−	M33BTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NFHiXGlKSzVyPUK0NE41KMLzIEK5MlAh\|ryP	M2m2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 wt	NGrEcmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NFXzV5BKSzVyPUGuN{DDuSByLkOgcm0>	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
L1210 10K	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NIfBUnNKSzVyPUKyMlIhyrFiMz63JO69VQ>?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
TC-1	NVTJWI1KT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MXfJR\|UxRTF2LkegxtEhOi56IH7N	MnziQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
TC-1-GR	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NGrZWlRKSzVyPUO2MlchyrFiNT6xJO69VQ>?	M1jW[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
MIA PaCa-2	MkK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NEfr[3BKSzVyPUS5MlchyrFiMUeuO{BvVQ>?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
PANC-1	NYX3WndlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		Mn;wTWM2OD1-IESwNEDPxE1?	MmPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
CCRF-CEM	M2\3e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MlyyTWM2OD1{LkmgxtEhOS56IH7N	NXW0O4pIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM-AraC-8C	M3jMbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NYTDflhEUUN3ME25PVgvQCEEsTC5MlQhdk1?	M1\IWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM	NHPmRXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHf1Uow4OiCq	M{PjbmlEPTB;Mj6wJOKyKDBwNjDuUS=>	Ml61QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NUG4OFMoRjJzOEWxPFQ{RC:jPh?=
MV-4-11	M4TMTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NVXXb4I4UUN3MDC9JFAvODByNTDuUS=>	MkW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ES4	NEexfINIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NVzDdnpwUUN3MDC9JFAvODByNzDuUS=>	NIfPbVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ACHN	M3;RT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MlL2TWM2OCB;IECuNFAxQSCwTR?=	NELqbmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KYSE-510	MoLuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MmfTTWM2OCB;IECuNFAyKG6P	MmjDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
EW-7	MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		Ml\WTWM2OCB;IECuNFAzPiCwTR?=	NUjteotqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
BFTC-905	NF[yeGNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NEDyRVlKSzVyIE2gNE4xODVzIH7N	NIP5RpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KE-37	MlSxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MY\JR\|UxKD1iMD6wNFU3KG6P	M{jJZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SBC-5	MnXHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MYjJR\|UxKD1iMD6wNFU4KG6P	NETOOZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NKM-1	MmT3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M1XiVmlEPTBiPTCwMlAxPzFibl2=	NV;xU\|c5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
RH-1	NHnNdJNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NX3pR25yUUN3MDC9JFAvODB5MjDuUS=>	M1ixd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ALL-PO	MmjUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NXv5[nFlUUN3MDC9JFAvODB6MzDuUS=>	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
QIMR-WIL	MnS2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NV\Ie4liUUN3MDC9JFAvODB6OTDuUS=>	NF;BR\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375	NHW4W\|RIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MYXJR\|UxKD1iMD6wNFk6KG6P	NEj0NHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SIG-M5	NULTZVlFT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MkDsTWM2OCB;IECuNFExPCCwTR?=	NXW2Wo9RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KGN	M{fGNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MVjJR\|UxKD1iMD6wNVA5KG6P	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-13	MmiwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M1zvVmlEPTBiPTCwMlAyOTJibl2=	M1WyNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
NCI-SNU-1	NH\yVZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M3z6[mlEPTBiPTCwMlAyPiCwTR?=	M{nvVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
PSN1	MnznS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NFnlVmlKSzVyIE2gNE4xOTZ3IH7N	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HUTU-80	NVviNHdET3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MVXJR\|UxKD1iMD6wNVY3KG6P	NEnDd5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
786-0	NGLBdlhIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MkCwTWM2OCB;IECuNFI{KG6P	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-16	MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NUC2W3pQUUN3MDC9JFAvODJ\|IH7N	NEO3ZlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ES1	NGrnRZdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MorkTWM2OCB;IECuNFI3QCCwTR?=	MnXhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RKO	NFryd\|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MYXJR\|UxKD1iMD6wNlc5KG6P	NIjwNIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ESS-1	NF;hS5FIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		Mnz1TWM2OCB;IECuNFI5PiCwTR?=	NHHwTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SK-UT-1	M3zwRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NEjEdmVKSzVyIE2gNE4xOjl5IH7N	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
LB2241-RCC	M2fvTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M3fxfGlEPTBiPTCwMlA{OThibl2=	M3PNXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
CHL-1	MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NG\CRYdKSzVyIE2gNE4xOzJ2IH7N	M{Pxd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SW1783	NFLiWopIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MX\JR\|UxKD1iMD6wN\|M3KG6P	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
MEL-JUSO	NX;TUphuT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M4j1emlEPTBiPTCwMlA{QTFibl2=	M4nsZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HT-29	NYDUVW5ET3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MnTzTWM2OCB;IECuNFQyOyCwTR?=	Mm\FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SNG-M	M1zsUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MmTYTWM2OCB;IECuNFQzPSCwTR?=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
TE-15	NV7HbHAzT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MmK1TWM2OCB;IECuNFQ3PCCwTR?=	NGr2T\|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HOS	MlX3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MYHJR\|UxKD1iMD6wOFghdk1?	NIPnSVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
BB65-RCC	M{LsdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NIfxNZJKSzVyIE2gNE4xPTF{IH7N	NGfzO5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HCE-4	MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NW\XV49HUUN3MDC9JFAvODV{ODDuUS=>	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
MHH-ES-1	NVXINWtpT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NH7IPZNKSzVyIE2gNE4xPTNzIH7N	NF3sbFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
RPMI-7951	M{fJb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NUL5ZYY1UUN3MDC9JFAvODV2MTDuUS=>	Ml7OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
IST-SL2	MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MoTlTWM2OCB;IECuNFU5PCCwTR?=	NFH1THg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
CMK	MmTZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MWTJR\|UxKD1iMD6wOVg3KG6P	MluxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
GR-ST	MnTJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M3vj[WlEPTBiPTCwMlA2QTVibl2=	Mlj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
NALM-6	NV\icIpjT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MUnJR\|UxKD1iMD6wOlIzKG6P	Mn;aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RPMI-6666	MmDmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MV;JR\|UxKD1iMD6wOlUzKG6P	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
LC-2-ad	M4DwWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MkLXTWM2OCB;IECuNFY2OyCwTR?=	NITveWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ARH-77	NYXNbVhXT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M{HaOWlEPTBiPTCwMlA4OTFibl2=	NHLVWG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
IST-MEL1	NFPrZ49Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NGW2RYVKSzVyIE2gNE4xPzJ4IH7N	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SW1710	MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M1W1[2lEPTBiPTCwMlA4PTFibl2=	Mn\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
DEL	NHuycm5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M3q1bWlEPTBiPTCwMlA5QDdibl2=	NIXTbm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
AGS	NFHY[3pIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NX;wdXE5UUN3MDC9JFAvODlyMjDuUS=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
NCI-H2122	M{DyPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MkCxTWM2OCB;IECuNFk1PSCwTR?=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HSC-4	Mn;RS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NFv5SZlKSzVyIE2gNE4yODJ{IH7N	NVfqfo9TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
AM-38	NVzlZnhsT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MmfBTWM2OCB;IECuNVIyPSCwTR?=	M1T1U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
769-P	NEXhPXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NYrBOGFDUUN3MDC9JFAvOTJ\|MTDuUS=>	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
RT-112	MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MVvJR\|UxKD1iMD6xNlc{KG6P	NIXXWnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
MCF7	NE\uTZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		Mk\YTWM2OCB;IECuNVM2QSCwTR?=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
IGROV-1	MkjxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MXHJR\|UxKD1iMD6xOFUhdk1?	NGXWXmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
OCI-AML2	M2q3fWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MUDJR\|UxKD1iMD6xOFY3KG6P	Mnz6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
NCI-H1299	M125dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MmTPTWM2OCB;IECuNVU3PiCwTR?=	Mn\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
A431	MmnZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NGS0TllKSzVyIE2gNE4yQDNzIH7N	Mn;aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SW982	M2X4bmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NF7aVIVKSzVyIE2gNE4zOTN\|IH7N	M4fzfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
BB30-HNC	NVXTfoJyT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NHqxOG9KSzVyIE2gNE4zOzF{IH7N	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ACN	NYj0PIlIT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MmTMTWM2OCB;IECuNlQ{PiCwTR?=	NFfOcYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
647-V	M3vNNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MnHwTWM2OCB;IECuNlQ5OSCwTR?=	M1zGU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SK-PN-DW	NWLreHNkT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MljZTWM2OCB;IECuNlY2PiCwTR?=	M16wcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
LCLC-97TM1	M1[wWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NFK3dJVKSzVyIE2gNE4zPjd\|IH7N	M2PqWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
LB1047-RCC	NIHMV3BIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NWXaN4NWUUN3MDC9JFAvOjZ6ODDuUS=>	MojDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
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CaR-1	NF73T3ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M3jzbGlEPTBiPTCwMlU2OTJ{IN88US=>	NEnyS3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
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Daudi	NI\TVHBIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NX7Zc205UUN3MDC9JFAvPTZ7NEmg{txO	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
CAL-120	MmrnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MYHJR\|UxKD1iMD61O\|U5QCEQvF2=	M2jwSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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OMC-1	M{HGVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MVrJR\|UxKD1iMD61PFM{OyEQvF2=	NVL5[mFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
U-266	NFPiTmZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MlLBTWM2OCB;IECuOlA4QTRizszN	NFPXdoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
OVCAR-4	MnTiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NUHxUpF5UUN3MDC9JFAvPjJ3MkOg{txO	Moe5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RCC10RGB	NI\0O\|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M{nwfGlEPTBiPTCwMlY{Ozd2IN88US=>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
NCI-H2141	Ml7vS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NWm2c3JFUUN3MDC9JFAvPjR2MESg{txO	M1SyNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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ChaGo-K-1	NXj5Z21CT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M13wNWlEPTBiPTCwMlY6PTZizszN	NF3GWok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
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UACC-62	M1\DXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M{jYTWlEPTBiPTCwMlgyOjZizszN	NIfkTms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
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NCI-H719	MmrLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NXnsTlVMUUN3MDC9JFAvQDlzNUeg{txO	M1;3SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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CAS-1	NUjIWIJUT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MXfJR\|UxKD1iMD65OVUxQCEQvF2=	M1\1blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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MHH-CALL-2	NHPHPZRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MmDFTWM2OCB;IEGuNFI{OTlizszN	MkHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
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TGBC24TKB	MnvCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NWLIZW1jUUN3MDC9JFEvODRzOEOg{txO	M130bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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A549	M1PV[2FvfGmycn;sbYZmemG2aY\lJIF{e2G7	Mmi5O\|IhcHK\|	MkezTWM2OCB;IECuNFIh\|ryP	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh4MUS5PUc,OjJ6NkG0PVk9N2F-
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Jurkat	M4XoV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7	Mn7FO\|IhcHK\|	NUfTWlhRUUN3MDC9JFAvODNizszN	NE\2cYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N\|c1OCd-MkC4O\|M4PDB:L3G+
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DU-145	NY\LTo8{S3m2b4TvfIlkcXS7IHHzd4F6		MkHJTWM2OCB;IECuNFM2PiEQvF2=	NX60V296RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3NlgyQTFpPkGxO\|I5OTlzPD;hQi=>
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MDA-MB-231	MVrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	MX[0PEBpenN?	MmXYTWM2OCB;IECuNFM3PCEQvF2=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd7NUe2O{c,Ojl5OUW3Olc9N2F-
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Jurkat	MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	NELHVoc4OiCqcoO=	NGP3eWdKSzVyIE2gNE4xPDV\|IN88US=>	NE\1Wow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES2PVgxQSd-MUi0Olk5ODl:L3G+
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K562	M3TJNGN6fG:2b4jpZ4l1gSCjc4PhfS=>	MV20PEBpenN?	MWXJR\|UxKD1iMD6wOUDPxE1?	NVn3VVFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N\|E{PTlpPkK0OlMyOzV7PD;hQi=>
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MIAPaCa2	NIPYepdEgXSxdH;4bYNqfHliYYPzZZk>	MoHUO\|IhcHK\|	MnXETWM2OCB;IECuNFYh\|ryP	M2qydFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUS0NVE6Lz5{Mkm0OFEyQTxxYU6=
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CEM	MoLYR5l1d3SxeHnjbZR6KGG\|c3H5	Mn7PN{Bl[Xm\|	NYHNPXdvUUN3MDC9JFAvODZ7IN88US=>	NIHKPXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0NVM2Pid-MkSzOFE{PTZ:L3G+
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K562	NEjYe5dEgXSxdH;4bYNqfHliYYPzZZk>	MWK3NkBpenN?	NYfaRnF5UUN3MDC9JFAvOSEQvF2=	M1\oTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkKxO\|kxLz5{OEKyNVc6ODxxYU6=
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RT112	MX\DfZRwfG:6aXPpeJkh[XO\|YYm=		NUPBZW1oTUN3MDC9JFAvOTB2OTFOwG0>	M{HqZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEexPVk5Lz5{NES3NVk6QDxxYU6=
PANC1	M4DvW2N6fG:2b4jpZ4l1gSCjc4PhfS=>	M2DkeFQ5KGi{cx?=	MVLJR\|UxKD1iMD6xNUDPxE1?	NF\lU3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO0NlE1Pid-MkKzOFIyPDZ:L3G+
MIAPaCa2	MlLTR5l1d3SxeHnjbZR6KGG\|c3H5	MkS2O\|IhcHK\|	M4DlcGlEPTBiPTCwMlEyKM7:TR?=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN{OE[1Okc,Ojl\|Mki2OVY9N2F-
MIAPaCa2	MoqxRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	NUPHZZRZPzBiaILz	NF7o[HZKSzVyIE2gNE4yOiEQvF2=	NWXVSIRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0O\|EyOTlpPkK5OFcyOTF7PD;hQi=>
CEM	MoO1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MonJTWM2OCB;IECuNVMh\|ryP	NVLSdJVoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
MCF7	NXXjWYlDS3m2b4TvfIlkcXS7IHHzd4F6	NIT4OVU{KGSjeYO=	MXTJR\|UxKD1iMD6xOFkh\|ryP	NFjW[289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
MCF7	Ml3GR5l1d3SxeHnjbZR6KGG\|c3H5	NVK5fJVqPzJiaILz	NIThc5hKSzVyIE2gNE4yPSEQvF2=	NFXRZVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCyOVg4PSd-Mk[wNlU5PzV:L3G+
CaCo2	MmXvRY51cXS3bX;yJIF{e2G7	MlPrOFghcHK\|	NVXV[JNZT0l3MDC9JFAvOThizszN	NInpdVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4PFI5OSd-MUi1PFgzQDF:L3G+
U2OS	NImyXVFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	Mm[yO\|IhcHK\|	MnrMTWM2OCB;IECuNVgh\|ryP	MnzCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
U2OS	M{TmZWN6fG:2b4jpZ4l1gSCjc4PhfS=>	NYXwXocxPzJiaILz	NIW3WFRKSzVyIE2gNE4yQCEQvF2=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ{MUe5NEc,Ojh{MkG3PVA9N2F-
MDA-MB-231	NIPOWYtEgXSxdH;4bYNqfHliYYPzZZk>	M13uTlI1KGi{cx?=	MYrJR\|UxKD1iMD6xPUDPxE1?	M1iyeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{CzNlk3Lz5{NUewN\|I6PjxxYU6=
H292	NFnwWY9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NWHpR\|hpUUN3MDC9JFAvOjFizszN	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
A549	MoLTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MkXuTWM2OCB;IECuNlI2KM7:TR?=	MlHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
NCI-H460	MXrDfZRwfG:6aXPpeJkh[XO\|YYm=	M4jYWVQ5KGi{cx?=	MV;JR\|UxKD1iMD6yN{DPxE1?	NVPGN2lyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOFIyPDZpPkKyN\|QzOTR4PD;hQi=>
MDA-MB-231	M{LWPWN6fG:2b4jpZ4l1gSCjc4PhfS=>	NVTDS2dFOyCmYYnz	NEHWcHVKSzVyIE2gNE4zPDVizszN	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
SW1573	MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MXnJR\|UxKD1iMD6yO\|Uh\|ryP	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
A2780	Mo\tR5l1d3SxeHnjbZR6KGG\|c3H5	MXi3NkBpenN?	MUXJR\|UxKD1iMD6zNUDPxE1?	NXiz[WdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N\|YzPDd2PD;hQi=>
K562	M1GyfWFvfGm2dX3vdkBie3OjeR?=	NGjEVoc1QCCqcoO=	NGPWbGpIUTVyIE2gNE4{OiEQvF2=	MmDxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3OEiyPFEoRjF6NUi4NlgyRC:jPh?=
HCT116	Ml33R5l1d3SxeHnjbZR6KGG\|c3H5	MYG0PEBpenN?	MYLJR\|UxKD1iMD6zNkDPxE1?	Ml\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|NEKxOFYoRjJ{M{SyNVQ3RC:jPh?=
CFPAC-1	NGnVR5FEgXSxdH;4bYNqfHliYYPzZZk>	MYq5OkBpenN?	MkPFTWM2OCB;IECuN\|Uh\|ryP	NHnKW\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zNVM2QSd-MkS2N\|E{PTl:L3G+
PANC1	NYrVS4FIS3m2b4TvfIlkcXS7IHHzd4F6	NF3XbGM4OiCqcoO=	NWL3NlJHUUN3MDC9JFAvPCEQvF2=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3NkKwNkc,Ojl4NU[yNFI9N2F-
HCT116	MVPDfZRwfG:6aXPpeJkh[XO\|YYm=	MoXWO\|IhcHK\|	MkP1TWM2OCB;IECuOFEh\|ryP	MknlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{MkG3PVAoRjJ6MkKxO\|kxRC:jPh?=
CFPAC-1	MUnDfZRwfG:6aXPpeJkh[XO\|YYm=	NVnLVGNjPzJiaILz	M3Lx[WlEPTBiPTCwMlQ4KM7:TR?=	NEO2[5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zNVM2QSd-MkS2N\|E{PTl:L3G+
ACHN	NYT2VXJOS3m2b4TvfIlkcXS7IHHzd4F6	MWW0PEBpenN?	NVfFVWw1UUN3MDC9JFAvPDhizszN	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2MkG0Okc,OjJ\|NEKxOFY9N2F-
HCT116-E6	MYDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	NF7kcpM4OiCqcoO=	M4TlNGlEPTBiPTCwMlUh\|ryP	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5M{e0NEc,OjB6N{O3OFA9N2F-
C6	NVPVOmpDS3m2b4TvfIlkcXS7IHHzd4F6	MlzsN{Bl[Xm\|	NI\WelZKSzVyIE2gNE42ODRizszN	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
LNCAP	NFzYOGJEgXSxdH;4bYNqfHliYYPzZZk>	MmfqN{Bl[Xm\|	NF\4eINKSzVyIE2gNE42OTJizszN	M1W0cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
Calu1	MlHWR5l1d3SxeHnjbZR6KGG\|c3H5	NYmxOWpPPDhiaILz	MoTMTWM2OCB;IECuOVIh\|ryP	NFLOOmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO0NlE1Pid-MkKzOFIyPDZ:L3G+
HT-29	NH7qTYVEgXSxdH;4bYNqfHliYYPzZZk>	MXG3NkBpenN?	NGH1ToRKSzVyIE2gNE42OiEQvF2=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB{NUi3OUc,OjZyMkW4O\|U9N2F-
MCF7	MXLDfZRwfG:6aXPpeJkh[XO\|YYm=	NHvLVpA4OiCqcoO=	M37lZmlEPTBiPTCwMlU4KM7:TR?=	NUPO[4V2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwO\|U2QTJpPkK4NFc2PTl{PD;hQi=>
K562	NXrY[2k3SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	NF;6SmI4OiCqcoO=	NYqyWm5MUUN3MDC9JFAvPiEQvF2=	Mk\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
MIAPaCa2	MU\DfZRwfG:6aXPpeJkh[XO\|YYm=	NYDw[HY1OjRiaILz	M2C0TmlEPTBiPTCwMlYh\|ryP	MlTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEOyPVYoRjJ3N{CzNlk3RC:jPh?=
MDA-MB-231	NYK0Wm46S3m2b4TvfIlkcXS7IHHzd4F6	NFTRUog4OiCqcoO=	NF3HNZhKSzVyIE2gNE43PSEQvF2=	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB{NUi3OUc,OjZyMkW4O\|U9N2F-
BxPC3	MmHMR5l1d3SxeHnjbZR6KGG\|c3H5	MlPCO\|IhcHK\|	NYnCemFNUUN3MDC9JFAvPjdizszN	NWDhdmVlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|E6QThpPkK0OFcyQTl6PD;hQi=>
K562	Mn[yR5l1d3SxeHnjbZR6KGG\|c3H5	M2O3PFMh\GG7cx?=	M3P2S2lEPTBiPTCwMlcyQCEQvF2=	Mm\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
K562	NGLkbHpCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	NIfnVpg4OiCqcoO=	MULJR\|UxKD1iMD63OFU6KM7:TR?=	M3yzR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NE[5PFA6Lz5zOES2PVgxQTxxYU6=
Bel7402	NYPzfWxlS3m2b4TvfIlkcXS7IHHzd4F6	M4\ZO\|czKGi{cx?=	NVLEN3RpUUN3MDC9JFAvQDRizszN	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl\|NkK0O\|Q9N2F-
HCT116/E6	NHvvSnVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	MXK3NkBpenN?	Ml2yTWM2OCB;IECuPFk3PSEQvF2=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR4OUiwPUc,OTh2Nkm4NFk9N2F-
HeLa	NWPUcXNNS3m2b4TvfIlkcXS7IHHzd4F6	M{TPdlk3KGi{cx?=	NIfzRo1KSzVyIE2gNE46KM7:TR?=	M1XFTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkOxN\|U6Lz5{NE[zNVM2QTxxYU6=
CEM	NGq4dI9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MnHPTWM2OCB;IEGg{txO	M2rwfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
HB-1	M1z4VmFvfGm4aYLhcEBie3OjeR?=	MmrhNlQhcHK\|	MUTFR\|UxKD1iMTFOwG0>	NGXrdpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEW4NFU2PCd-MkC1PFA2PTR:L3G+
MIAPaCa2	NIjh[XZEgXSxdH;4bYNqfHliYYPzZZk>	MWC3NkBpenN?	NY\GfIRlUUN3MDC9JFEvODRizszN	MmXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{G5PVgoRjJ2NEexPVk5RC:jPh?=
SK-N-AS	MnP3R5l1d3SxeHnjbZR6KGG\|c3H5	MkGzN{Bl[Xm\|	MnPXTWM2OCB;IEGuNUDPxE1?	M{C3TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990	NYDpZ\|B7S3m2b4TvfIlkcXS7IHHzd4F6	MYq3NkBpenN?	M1;tOGlEPTBiPTCxMlIh\|ryP	M3TTR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
A549	MlHwR5l1d3SxeHnjbZR6KGG\|c3H5	Ml\LO\|IhcHK\|	MXzJR\|UxKD1iMT60JO69VQ>?	Mn\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|NkK0O\|QoRjF7M{[yOFc1RC:jPh?=
HepG2	NXK5PJFyTnWwY4Tpc44h[XO\|YYm=		MnTtT40hRSBzLkSg{txO	Mnz6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzMEG2O\|QoRjF5MUCxOlc1RC:jPh?=
BL21(DE3)	MXvGeY5kfGmxbjDhd5NigQ>?		M4HsOWtuKD1iMT60N{DPxE1?	NIXJdWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN\|AyOzF:L3G+
U87MG	M2TXXmN6fG:2b4jpZ4l1gSCjc4PhfS=>	NFrZcVE{KGSjeYO=	NYO3RZhwUUN3MDC9JFEvPDlizszN	M3XhWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
BL21(DE3)	MoOxSpVv[3Srb36gZZN{[Xl?		MVTLcUA:KDFwNUKg{txO	M4iwOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkOwNVMyLz5{M{KzNFE{OTxxYU6=
HT-29	NIK5V5BEgXSxdH;4bYNqfHliYYPzZZk>	NXTIZWFTOyCmYYnz	MV\JR\|UxKD1iMT61N{DPxE1?	NWPUfGI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO\|EyODV2PD;hQi=>
SW1990	MlWxR5l1d3SxeHnjbZR6KGG\|c3H5		NGjKSo9KSzVyIE2gNU43KM7:TR?=	M2LNcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUm1OFY3Lz5{NEG5OVQ3PjxxYU6=
Capan2	NXTLbmRqS3m2b4TvfIlkcXS7IHHzd4F6	MYO3NkBpenN?	NFnhbJNKSzVyIE2gNU44KM7:TR?=	MlvFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|NkK0O\|QoRjF7M{[yOFc1RC:jPh?=
SW480	NF;IVndCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	Mk\SO\|IhcHK\|	MX7JR\|UxKD1iMT63JO69VQ>?	M37YOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEezO\|QxLz5{MEi3N\|c1ODxxYU6=
PANC1	NWnJNnVDS3m2b4TvfIlkcXS7IHHzd4F6	NED5THg4OiCqcoO=	NVv6WIRPUUN3MDC9JFEvPyEQvF2=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3NkKwNkc,Ojl4NU[yNFI9N2F-
HCT8	NYnrWZh6S3m2b4TvfIlkcXS7IHHzd4F6	M1PQdVczKGi{cx?=	MmPpTWM2OCB;IEGuO\|Qh\|ryP	Mo\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|NkK0O\|QoRjF7M{[yOFc1RC:jPh?=
BL21(DE3)	MnLYSpVv[3Srb36gZZN{[Xl?		M3nQeGtuKD1iMT63OUDPxE1?	NGnIS\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN\|AyOzF:L3G+
HT-29	M2PCZWN6fG:2b4jpZ4l1gSCjc4PhfS=>	MoHFOFghcHK\|	NYLBVHNsUUN3MDC9JFEvQTVizszN	MlHZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ\|OU[4PFI1RC:jPh?=
MIAPaCa2	M4PhbmN6fG:2b4jpZ4l1gSCjc4PhfS=>	NIHoeoI2KGSjeYO=	NIW2NlRGSzVyIE2gNkDPxE1?	M{XJW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OE[3OVkxLz5{NEi2O\|U6ODxxYU6=
BL21(DE3)	MVXGeY5kfGmxbjDhd5NigQ>?	NV[4RlY1OSCvaX6=	NFG0N2hMdSB;IEKuNVUh\|ryP	Mm\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN\|EoRjJ\|MkOwNVMyRC:jPh?=
SW1990	MoK3R5l1d3SxeHnjbZR6KGG\|c3H5		M1G5XmlEPTBiPTCyMlIh\|ryP	M3XtU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUC1O\|IzLz5{NUGwOVczOjxxYU6=
SW1990	MXHDfZRwfG:6aXPpeJkh[XO\|YYm=	NVfuPINbPDhiaILz	NYnJPWxOUUN3MDC9JFIvOyEQvF2=	NUnRR\|ZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke5OlY6PTBpPkK3PVY3QTVyPD;hQi=>
NCI-H146	M2jSS2N6fG:2b4jpZ4l1gSCjc4PhfS=>	MVGzJIRigXN?	MV;JR\|UxKD1iMj63PEDPxE1?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
BxPC3	M2\pT2N6fG:2b4jpZ4l1gSCjc4PhfS=>	MUK3NkBpenN?	M4frTmlEPTBiPTCyMlkh\|ryP	M3y1RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
p53 deficient COLO205	M3H2emFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NFLJTXo4OiCqcoO=	M3POTmlEPTBiPTCzJO69VQ>?	NGryNHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N\|c1OCd-MkC4O\|M4PDB:L3G+
MiaPaCa	NVLEc\|ByS3m2b4TvfIlkcXS7IHHzd4F6	Ml25OkBl[Xm\|	MWfJR\|UxKD1iMzFOwG0>	NIHrVW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O2NFExPCd-MkOzOlAyODR:L3G+
COLO205	Mlz2R5l1d3SxeHnjbZR6KGG\|c3H5	NIfqOGQ1QCCqcoO=	NWG1XoxJUUN3MDC9JFMvOjRizszN	M17VVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OU[4PFI1Lz5{M{m2PFgzPDxxYU6=
MCF7	MX7DfZRwfG:6aXPpeJkh[XO\|YYm=	MnzjO\|IhcHK\|	NF7IWmtKSzVyIE2gN{4zQCEQvF2=	M372RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
MIAPaCa2	MmTER5l1d3SxeHnjbZR6KGG\|c3H5	NET4TWE4OiCqcoO=	MYTJR\|UxKD1iMz6zJO69VQ>?	M17sfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{K4OlU3Lz5{OUOyPFY2PjxxYU6=
HeLa	NH62c4ZEgXSxdH;4bYNqfHliYYPzZZk>	M{fVdFI1KGi{cx?=	NXHGXng6UUN3MDC9JFMvOyEQvF2=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyM{K5Okc,OjV5MEOyPVY9N2F-
FTC-133	NInXb5JEgXSxdH;4bYNqfHliYYPzZZk>	MUmyOEBpenN?	M4jQZ2lEPTBiPTCzMlM3KM7:TR?=	MnPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3	NXLOPVZiT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	NU\vSGV3PDhiaILz	NHr3dGVIUTVyIE2gN{43PCEQvF2=	NGK0Omg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+
COLO320DM	Mmm1R5l1d3SxeHnjbZR6KGG\|c3H5	NIDLbFY1QCCqcoO=	M4qwNGlEPTBiPTCzMlkzKM7:TR?=	MnnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ\|OU[4PFI1RC:jPh?=
HeLa	NXr0Z3lLS3m2b4TvfIlkcXS7IHHzd4F6	MXSzJIRigXN?	NHXEeYdKSzVyIE2gOE4yOiEQvF2=	M1\nVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
8305C	NVe5RmwxS3m2b4TvfIlkcXS7IHHzd4F6	M4HpRVI1KGi{cx?=	MUTJR\|UxKD1iND61N{DPxE1?	NUizOo1LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N\|Y6QTRpPkK0OFM3QTl2PD;hQi=>
PANC1	NEK3em9EgXSxdH;4bYNqfHliYYPzZZk>	NHLqTIs4OiCqcoO=	NEPISoNKSzVyIE2gOU43KM7:TR?=	NUPCPWhQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N\|YzPDd2PD;hQi=>
PANC1	MnLoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	NGPJNGE4OiCqcoO=	NYW2PFRTT0l3MDC9JFUvQCEQvF2=	NX\rNIF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0PVUxQDFpPkK4OFk2ODhzPD;hQi=>
SK-MEL-2	MXrDfZRwfG:6aXPpeJkh[XO\|YYm=	NHPxZm8{KGSjeYO=	MWfJR\|UxKD1iNz6xNUDPxE1?	M2LQV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
CEM	MmDWR5l1d3SxeHnjbZR6KGG\|c3H5	NV3QVZdIOyCmYYnz	M1rp[mlEPTBiPTC3MlYh\|ryP	MoDNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NEGzOVYoRjJ2M{SxN\|U3RC:jPh?=
SW1573	NFTjWZBEgXSxdH;4bYNqfHliYYPzZZk>	MnLqO\|IhcHK\|	M{T5SWlEPTBiPTC4MlMh\|ryP	NXrUeIlKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVk3ODRpPkG3OFE6PjB2PD;hQi=>
BxPC3	M1fzNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NUTyemVHPDhiaILz	MWTMR\|UxKD1iOD63NUDPxE1?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVzMkmwPEc,OjJ3MUK5NFg9N2F-
MCF7	Ml;iR5l1d3SxeHnjbZR6KGG\|c3H5	MXW3NkBpenN?	MXLJR\|UxKD1iOD65JO69VQ>?	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB5NUW5Nkc,OjhyN{W1PVI9N2F-
BJ	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=	M{HqN\|Mh\GG7cx?=	NIn2cYpKSzVyIE2gPU45QCEQvF2=	NFe3TIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
HeLa	NHiybWJEgXSxdH;4bYNqfHliYYPzZZk>	NYP5dos6PzJiaILz	M1T1WmlEPTBiPTCxNEDPxE1?	MmrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{GzOVkoRjJ2NkOxN\|U6RC:jPh?=
B16-F10-Luc-G5	M4XMN2FvfGmycn;sbYZmemG2aY\lJIF{e2G7	MnOwNlQhcHK\|	NUTTW4NYUUN3MDC9JFEyKM7:TR?=	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN2OUOzNkc,Ojd\|NEmzN\|I9N2F-
HCT15	NVzyeWtES3m2b4TvfIlkcXS7IHHzd4F6		NGC3[2VKSzVyIE2gNVEvQCEQvF2=	NUXv[5hHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixPFY3ODRpPkG4NVg3PjB2PD;hQi=>
A549	MmXPR5l1d3SxeHnjbZR6KGG\|c3H5	MXq3NkBpenN?	MXXJR\|UxKD1iMUOuNUDPxE1?	M2fFdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
HCT116	NXLQVlczS3m2b4TvfIlkcXS7IHHzd4F6		M1LvU2lEPTBiPTCxOE4{KM7:TR?=	NETBc5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG4OlYxPCd-MUixPFY3ODR:L3G+
MiaPaCa	MVjDfZRwfG:6aXPpeJkh[XO\|YYm=	NVL1NHRmPzJiaILz	M1i0bmlEPTBiPTCxO{4yKM7:TR?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR6OU[yOkc,OjN2OEm2NlY9N2F-
AG6000	NHKyU\|BIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MXPJR\|UxKD1iMkCg{txO	MlTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
AG6000	NGfHdlJIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NH;JU25KSzVyIE2gNlAh\|ryP	MnruQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
BxPC3	Mm[zRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	NXvGU2RVPzJiaILz	MlnCTWM2OCB;IEKwMlIh\|ryP	NVrlRnZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O\|Y3OzNpPkK4OVc3PjN\|PD;hQi=>
CV1	NUDRZ\|B[S3m2b4TvfIlkcXS7IHHzd4F6	MmfiO\|IhcHK\|	NH70fJBKSzVyIE2gNlEvQSEQvF2=	MkXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEm2NlYoRjJ\|NEi5OlI3RC:jPh?=
AsPC1	M2j5O2FvfGmycn;sbYZmemG2aY\lJIF{e2G7	MUW3NkBpenN?	NXnvenZIUUN3MDC9JFI3NjhizszN	NI\Ccnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O\|Y3OzN:L3G+
PANC1	M{fZZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7	MUS3NkBpenN?	MYLJR\|UxKD1iM{CuOEDPxE1?	NH;qO489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O\|Y3OzN:L3G+
MIAPaCa2	MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NEDzfGU1QCCqcoO=	NIfiRWlIUTVyIE2gN\|UvQDNizszN	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVzMkmwPEc,OjJ3MUK5NFg9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p70S6K1 / p-S6 / HIF-1α; PubMed: 27765914 Oncotarget Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control. LC3-I / LC3-II; PubMed: 27384485 Oncotarget A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h). pS-ERα / ERα / pERK / ERK; PubMed: 27384485 Oncotarget MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected. caspase-3 / caspase-8 / caspase-9; PubMed: 16307741 FEBS Lett H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control. p-JNK / JNK / p-ATF2 / p-c-Jun; PubMed: 16307741 FEBS Lett Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h.	27765914 27384485 16307741
Immunofluorescence	BMI1; PubMed: 27177084 Oncotarget Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining.	27177084
Growth inhibition assay	Cell viability ; PubMed: 27765914 Oncotarget A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.	27765914

In vivo

Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. ^[2]

Protocol

Cell Research:^[2]

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Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
Concentrations: 0.2 μM
Incubation Time: 24 hours or 48 hours
Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
(Only for Reference)

Animal Research:^[2]

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Animal Models: Athymic nude mice with MIA PaCa-2 cells
Dosages: 50 mg/kg or 100 mg/kg
Administration: Administered via i.p.
(Only for Reference)

References

Solubility (25°C)

In vitro	Water	19 mg/mL (63.4 mM)
	DMSO	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): Saline For best results, use promptly after mixing.	19mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Gemcitabine HCl SDF

Molecular Weight	299.66
Formula	C₉H₁₁F₂N₃O₄.HCI
CAS No.	122111-03-9
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	LY188011
Smiles	C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04634539	Not yet recruiting	Drug: Gemcitabine\|Drug: Nab-paclitaxel\|Drug: L-glutamine	Advanced Pancreatic Adenocarcinoma\|Pancreatic Cancer\|Pancreatic Ductal Adenocarcinoma	Jun Gong MD\|Emmaus Medical Inc.\|Cedars-Sinai Medical Center	January 1 2021	Phase 1
NCT04383119	Not yet recruiting	Drug: Trabectedin\|Drug: Gemcitabine	Leiomyosarcoma of Ovary\|Soft Tissue Sarcoma	Italian Sarcoma Group\|PharmaMar	January 15 2021	Phase 2
NCT04616534	Not yet recruiting	Drug: Elimusertib\|Drug: Gemcitabine	Advanced Fallopian Tube Carcinoma\|Advanced Malignant Solid Neoplasm\|Advanced Ovarian Carcinoma\|Advanced Pancreatic Adenocarcinoma\|Advanced Primary Peritoneal Carcinoma\|High Grade Ovarian Serous Adenocarcinoma\|Platinum-Resistant Fallopian Tube Carcinoma\|Platinum-Resistant Ovarian Carcinoma\|Platinum-Resistant Primary Peritoneal Carcinoma\|Stage II Pancreatic Cancer AJCC v8\|Stage IIA Pancreatic Cancer AJCC v8\|Stage IIB Pancreatic Cancer AJCC v8\|Stage III Fallopian Tube Cancer AJCC v8\|Stage III Ovarian Cancer AJCC v8\|Stage III Pancreatic Cancer AJCC v8\|Stage III Primary Peritoneal Cancer AJCC v8\|Stage IIIA Fallopian Tube Cancer AJCC v8\|Stage IIIA Ovarian Cancer AJCC v8\|Stage IIIA Primary Peritoneal Cancer AJCC v8\|Stage IIIA1 Fallopian Tube Cancer AJCC v8\|Stage IIIA1 Ovarian Cancer AJCC v8\|Stage IIIA2 Fallopian Tube Cancer AJCC v8\|Stage IIIA2 Ovarian Cancer AJCC v8\|Stage IIIB Fallopian Tube Cancer AJCC v8\|Stage IIIB Ovarian Cancer AJCC v8\|Stage IIIB Primary Peritoneal Cancer AJCC v8\|Stage IIIC Fallopian Tube Cancer AJCC v8\|Stage IIIC Ovarian Cancer AJCC v8\|Stage IIIC Primary Peritoneal Cancer AJCC v8\|Stage IV Fallopian Tube Cancer AJCC v8\|Stage IV Ovarian Cancer AJCC v8\|Stage IV Pancreatic Cancer AJCC v8\|Stage IV Primary Peritoneal Cancer AJCC v8\|Stage IVA Fallopian Tube Cancer AJCC v8\|Stage IVA Ovarian Cancer AJCC v8\|Stage IVA Primary Peritoneal Cancer AJCC v8\|Stage IVB Fallopian Tube Cancer AJCC v8\|Stage IVB Ovarian Cancer AJCC v8\|Stage IVB Primary Peritoneal Cancer AJCC v8	National Cancer Institute (NCI)	December 31 2020	Phase 1
NCT04657068	Not yet recruiting	Drug: ART0380\|Drug: Gemcitabine	Advanced Cancer\|Metastatic Cancer\|Ovarian Cancer	Artios Pharma Ltd	December 2020	Phase 1\|Phase 2
NCT04640623	Not yet recruiting	Drug: TAR-200\|Drug: Cetrelimab	Urinary Bladder Neoplasms	Janssen Research & Development LLC	December 17 2020	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
What’s the difference between S1714 and S1149 and which one is better?
Answer:
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

DNA/RNA Synthesis Signaling Pathway Map

Related DNA/RNA Synthesis Products

SCR7 ^New

SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ). It increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos.
Blasticidin S HCl ^New

Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes, and acts as a DNA and protein synthesis inhibitor, used to select transfected cells carrying bsr or BSD resistance genes.
YK-4-279 ^New

YK-4-279 is a potent inhibitor of EWS-FLI1 binding to RNA helicase A (RHA).
Cisplatin

Cisplatin (cisplatinum, cis-diamminedichloroplatinum II, CDDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy. Solutions are best fresh-prepared.

Features:One of the most widely used and most potent chemotherapeutic agents.
Oxaliplatin

Oxaliplatin (L-OHP) is a DNA alkylating agent that activates autophagy. Oxaliplatin inhibits DNA synthesis by conforming DNA adducts in RT4, TCCSUP, A2780, HT-29, U-373MG, U-87MG, SK-MEL-2, and HT-144 cells. DMF is recommended for dissolution. Solutions are best fresh-prepared.
Bleomycin Sulfate

Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.
Gemcitabine

Gemcitabine (LY-188011, NSC 613327), a nucleic acid synthesis inhibitor, is a very potent and specific deoxycytidine analogue, used as chemotherapy. Gemcitabine induces a potent p53-dependent apoptosis.
Fluorouracil (5-Fluorouracil, 5-FU)

Fluorouracil (5-Fluorouracil, 5-FU, NSC 19893) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells. Fluorouracil induces apoptosis and can be used in the treatment of HIV.
Carboplatin

Carboplatin (JM-8, CBDCA, NSC 241240) is a DNA synthesis inhibitor by binding to DNA and interfering with the cell's repair mechanism in A2780, SKOV-3, IGROV-1, and HX62 cells. Solutions are best fresh-prepared.

Features:A DNA synthesis inhibitor.
Cytarabine

Cytarabine (Cytarabin, Ara-C, Arabinofuranosyl Cytidine) is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells. Cytarabine induces autophagy and apoptosis.

Features:The 1st of a series of cancer drugs that alters the sugar component of nucleosides.

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Gemcitabine HCl