Gemcitabine (LY-188011) HCl

For research use only.

Catalog No.S1149 Synonyms: NSC 613327

116 publications

Gemcitabine (LY-188011) HCl Chemical Structure

CAS No. 122111-03-9

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Selleck's Gemcitabine (LY-188011) HCl has been cited by 116 publications

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
Features Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.
Targets
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTJwOTFCtUAyNjhibl2= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM/dCK−/− NHfiXYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTJ2MD60JOKyKDJ7LkCg{txO NX3lZ25iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 wt MomyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLCTWM2OD1zLkOgxtEhOC5|IH7N M13ZPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 10K NX:zO5ZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTJ{LkKgxtEhOy55IN88US=> NGTXRoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
TC-1  NYKwPY5TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTRSWZKSzVyPUG0MlchyrFiMj64JI5O NYfSR2pmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
TC-1-GR NGXxeYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojlTWM2OD1|Nj63JOKyKDVwMTFOwG0> M1fBbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
MIA PaCa-2 NGPibXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fub2lEPTB;NEmuO{DDuSBzNz63JI5O MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
PANC-1 M373Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHCTWM2OD1-IESwNEDPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM MljHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LOZWlEPTB;Mj65JOKyKDFwODDuUS=> NFzpZm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM-AraC-8C NWX1OolOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHQRZlKSzVyPUm5PE45KMLzIEmuOEBvVQ>? M4XUd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM  MoLRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorsO|IhcA>? NY[yVWxWUUN3ME2yMlAhyrFiMD62JI5O NEXyNZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVg1Oyd-MkG4OVE5PDN:L3G+
MV-4-11 NUHkPVBDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF7B[|VKSzVyIE2gNE4xODB3IH7N MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ES4 MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{nJ[2lEPTBiPTCwMlAxODdibl2= NIXBV2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ACHN MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NI\mVHFKSzVyIE2gNE4xODB7IH7N MkHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
KYSE-510 Mor5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUHUUoI{UUN3MDC9JFAvODBzIH7N MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-7 MnS3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV3KTnlxUUN3MDC9JFAvODB{NjDuUS=> NUT2enIyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
BFTC-905 NEHIbHhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYnEeGFNUUN3MDC9JFAvODB3MTDuUS=> M2WxfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
KE-37 NYDmN451T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWLMdVNqUUN3MDC9JFAvODB3NjDuUS=> NXz1ZpczRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
SBC-5 M3XxdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4nMc2lEPTBiPTCwMlAxPTdibl2= NUPOPHRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NKM-1 MkewS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV7JR|UxKD1iMD6wNFcyKG6P M1LKbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
RH-1 M1jhb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXzJR|UxKD1iMD6wNFczKG6P MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ALL-PO M371cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYrRfm1pUUN3MDC9JFAvODB6MzDuUS=> NILYcm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
QIMR-WIL MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJR|UxKD1iMD6wNFg6KG6P MnnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
A375 NYSzcnNwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfad|lKSzVyIE2gNE4xODl7IH7N MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SIG-M5 MmrKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU\JR|UxKD1iMD6wNVA1KG6P NUnPVpR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KGN NYfNWFExT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkDSTWM2OCB;IECuNFExQCCwTR?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-13 NXfuUpN[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3nVfGlEPTBiPTCwMlAyOTJibl2= M3PDWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
NCI-SNU-1 M{fvT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnrWTWM2OCB;IECuNFE3KG6P Mk[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
PSN1 M1ezSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkToTWM2OCB;IECuNFE3PSCwTR?= NIXkXno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HUTU-80 NH\tTHJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVnJR|UxKD1iMD6wNVY3KG6P NYHZR4hlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
786-0 MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYnJR|UxKD1iMD6wNlMhdk1? Mm[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
EW-16 M{fWSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUXlfW1CUUN3MDC9JFAvODJ|IH7N NV3oRoNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ES1 Mm\HS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4DTT2lEPTBiPTCwMlAzPjhibl2= MnO1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RKO NVX3SpR1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NILGNW1KSzVyIE2gNE4xOjd6IH7N MlHuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ESS-1 M1\pdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1XGcGlEPTBiPTCwMlAzQDZibl2= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SK-UT-1 NVzjRXhZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXzJR|UxKD1iMD6wNlk4KG6P NYq3NVRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
LB2241-RCC NHLpU|hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1XYNWlEPTBiPTCwMlA{OThibl2= NVmz[pI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
CHL-1 NECyWo1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWPZeoE3UUN3MDC9JFAvODN{NDDuUS=> M4XkXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SW1783 NI\GdG9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzHTWM2OCB;IECuNFM{PiCwTR?= NYjpeW5uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
MEL-JUSO MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2rY[2lEPTBiPTCwMlA{QTFibl2= NVjXN3djRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
HT-29 MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXf4emx4UUN3MDC9JFAvODRzMzDuUS=> NHewToM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SNG-M M2W2fmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFnaSVhKSzVyIE2gNE4xPDJ3IH7N NVPRXIJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
TE-15 MlPDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2[0ZWlEPTBiPTCwMlA1PjRibl2= MlLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
HOS MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUjJR|UxKD1iMD6wOFghdk1? M3L3UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
BB65-RCC NVvnUJhHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF7pUoJKSzVyIE2gNE4xPTF{IH7N MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HCE-4 M17wR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVPnOHRCUUN3MDC9JFAvODV{ODDuUS=> NE\icVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
MHH-ES-1 MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYfJR|UxKD1iMD6wOVMyKG6P NVPWU45RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
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BL21(DE3) M1jkfGZ2dmO2aX;uJIF{e2G7 MlnLT40hRSBzLkSzJO69VQ>? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ|MEGzNUc,OjN{M{CxN|E9N2F-
U87MG MYHDfZRwfG:6aXPpeJkh[XO|YYm= M1[4WlMh\GG7cx?= NUnLcVFiUUN3MDC9JFEvPDlizszN MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
BL21(DE3) MX3GeY5kfGmxbjDhd5NigQ>? MVLLcUA:KDFwNUKg{txO MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ|MEGzNUc,OjN{M{CxN|E9N2F-
HT-29 MlTYR5l1d3SxeHnjbZR6KGG|c3H5 M4HSdlMh\GG7cx?= NH7qSmpKSzVyIE2gNU42OyEQvF2= M33IcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990 NV\UTI5oS3m2b4TvfIlkcXS7IHHzd4F6 MXLJR|UxKD1iMT62JO69VQ>? NIXi[oY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG5OVQ3Pid-MkSxPVU1PjZ:L3G+
Capan2 MWnDfZRwfG:6aXPpeJkh[XO|YYm= M3THWFczKGi{cx?= M4XEUGlEPTBiPTCxMlch|ryP M1HpS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
SW480 M3ToPGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnLaO|IhcHK| NH;TTmJKSzVyIE2gNU44KM7:TR?= NXLOcmFMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4O|M4PDBpPkKwPFc{PzRyPD;hQi=>
PANC1 NEHEe4REgXSxdH;4bYNqfHliYYPzZZk> NHrxb2Y4OiCqcoO= Mn64TWM2OCB;IEGuO{DPxE1? M{K0O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkW2NlAzLz5{OU[1OlIxOjxxYU6=
HCT8 MX3DfZRwfG:6aXPpeJkh[XO|YYm= NHXRbJY4OiCqcoO= M1GwdGlEPTBiPTCxMlc1KM7:TR?= MmHZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
BL21(DE3) MXvGeY5kfGmxbjDhd5NigQ>? MYHLcUA:KDFwN{Wg{txO NYTRb41CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|AyOzFpPkKzNlMxOTNzPD;hQi=>
HT-29 NXLUcYFNS3m2b4TvfIlkcXS7IHHzd4F6 M3;DVlQ5KGi{cx?= MVzJR|UxKD1iMT65OUDPxE1? NHTxPIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MIAPaCa2 MWTDfZRwfG:6aXPpeJkh[XO|YYm= NVq0eZBKPSCmYYnz M2XwN2VEPTBiPTCyJO69VQ>? M3naUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OE[3OVkxLz5{NEi2O|U6ODxxYU6=
BL21(DE3) NY\0N3BsTnWwY4Tpc44h[XO|YYm= MWqxJI1qdg>? NHLLN4FMdSB;IEKuNVUh|ryP MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ|MEGzNUc,OjN{M{CxN|E9N2F-
SW1990 NY\ocpI2S3m2b4TvfIlkcXS7IHHzd4F6 M2fMUGlEPTBiPTCyMlIh|ryP NV7WU5pIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNFU4OjJpPkK1NVA2PzJ{PD;hQi=>
SW1990 MUnDfZRwfG:6aXPpeJkh[XO|YYm= MVS0PEBpenN? NIHCN3RKSzVyIE2gNk4{KM7:TR?= NEm1ZVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{m2Olk2OCd-Mke5OlY6PTB:L3G+
NCI-H146 MlG0R5l1d3SxeHnjbZR6KGG|c3H5 NYL1cohJOyCmYYnz NYPweYhiUUN3MDC9JFIvPzhizszN M4X3ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
BxPC3 M336UmN6fG:2b4jpZ4l1gSCjc4PhfS=> NVzIc5V2PzJiaILz MVnJR|UxKD1iMj65JO69VQ>? MlrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
p53 deficient COLO205 Mn;aRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MlrKO|IhcHK| NHfofFlKSzVyIE2gN{DPxE1? NVy4O2Y2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4O|M4PDBpPkKwPFc{PzRyPD;hQi=>
MiaPaCa MlLqR5l1d3SxeHnjbZR6KGG|c3H5 MVu2JIRigXN? MlLtTWM2OCB;IEOg{txO M1PpZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{[wNVA1Lz5{M{O2NFExPDxxYU6=
COLO205 NEHw[W1EgXSxdH;4bYNqfHliYYPzZZk> NH\SepU1QCCqcoO= MnW2TWM2OCB;IEOuNlQh|ryP NEnUNZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MCF7 M2T4bGN6fG:2b4jpZ4l1gSCjc4PhfS=> NIfibWE4OiCqcoO= NWHBbYtNUUN3MDC9JFMvOjhizszN M17rN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
MIAPaCa2 Mn;2R5l1d3SxeHnjbZR6KGG|c3H5 NHXhPVA4OiCqcoO= NUn6NZZiUUN3MDC9JFMvOyEQvF2= M1XaXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{K4OlU3Lz5{OUOyPFY2PjxxYU6=
HeLa NXTMdYlxS3m2b4TvfIlkcXS7IHHzd4F6 Ml:0NlQhcHK| MWXJR|UxKD1iMz6zJO69VQ>? M{TRSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{CzNlk3Lz5{NUewN|I6PjxxYU6=
FTC-133 MljuR5l1d3SxeHnjbZR6KGG|c3H5 MUOyOEBpenN? MU\JR|UxKD1iMz6zOkDPxE1? M4THWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEO2PVk1Lz5{NESzOlk6PDxxYU6=
BxPC3 NWCxSXJKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4H2VlQ5KGi{cx?= NXmxS3BwT0l3MDC9JFMvPjRizszN M3GzNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
COLO320DM NFTBc3hEgXSxdH;4bYNqfHliYYPzZZk> M3jMVFQ5KGi{cx?= M3P5ZmlEPTBiPTCzMlkzKM7:TR?= NHTSXI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
HeLa NEHMeIFEgXSxdH;4bYNqfHliYYPzZZk> NFnCbng{KGSjeYO= MUfJR|UxKD1iND6xNkDPxE1? M1zYW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
8305C MnvLR5l1d3SxeHnjbZR6KGG|c3H5 M4XkNlI1KGi{cx?= NWL3blZwUUN3MDC9JFQvPTNizszN M3L0TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEO2PVk1Lz5{NESzOlk6PDxxYU6=
PANC1 M{LIUGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVe2PGFYPzJiaILz MXnJR|UxKD1iNT62JO69VQ>? M3:wWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
PANC1 MojJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnXlO|IhcHK| MknrS2k2OCB;IEWuPEDPxE1? MmHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2OUWwPFEoRjJ6NEm1NFgyRC:jPh?=
SK-MEL-2 NVnQW5B4S3m2b4TvfIlkcXS7IHHzd4F6 NGXMdnY{KGSjeYO= MlLPTWM2OCB;IEeuNVEh|ryP MofLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
CEM NEjaSWlEgXSxdH;4bYNqfHliYYPzZZk> M4LofVMh\GG7cx?= NWrXWlFSUUN3MDC9JFcvPiEQvF2= NHTNdFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0NVM2Pid-MkSzOFE{PTZ:L3G+
SW1573 MYPDfZRwfG:6aXPpeJkh[XO|YYm= MYe3NkBpenN? MljTTWM2OCB;IEiuN{DPxE1? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzOU[wOEc,OTd2MUm2NFQ9N2F-
BxPC3 NHfscIVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHj5SY41QCCqcoO= MmTHUGM2OCB;IEiuO|Eh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVzMkmwPEc,OjJ3MUK5NFg9N2F-
MCF7 MVjDfZRwfG:6aXPpeJkh[XO|YYm= MX:3NkBpenN? Mn;aTWM2OCB;IEiuPUDPxE1? NGrPPI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC3OVU6Oid-MkiwO|U2QTJ:L3G+
BJ NHTXb21EgXSxdH;4bYNqfHliYYPzZZk> M3fDXlMh\GG7cx?= M{DaZ2lEPTBiPTC5Mlg5KM7:TR?= M4HUWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
HeLa MY\DfZRwfG:6aXPpeJkh[XO|YYm= NF3TV3E4OiCqcoO= NWm1WWVVUUN3MDC9JFExKM7:TR?= NInzPWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zNVM2QSd-MkS2N|E{PTl:L3G+
B16-F10-Luc-G5 MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{\1[VI1KGi{cx?= NXHDTm1zUUN3MDC9JFEyKM7:TR?= NXj6[49ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezOFk{OzJpPkK3N|Q6OzN{PD;hQi=>
HCT15 MYnDfZRwfG:6aXPpeJkh[XO|YYm= NEPjb5hKSzVyIE2gNVEvQCEQvF2= MlrSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzOE[2NFQoRjF6MUi2OlA1RC:jPh?=
A549 MV\DfZRwfG:6aXPpeJkh[XO|YYm= M1vUXVczKGi{cx?= MY\JR|UxKD1iMUOuNUDPxE1? NVPuOHB5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVk3ODRpPkG3OFE6PjB2PD;hQi=>
HCT116 NU\F[4xHS3m2b4TvfIlkcXS7IHHzd4F6 NXTTZ|ZPUUN3MDC9JFE1NjNizszN M1WxU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MUi2OlA1Lz5zOEG4OlYxPDxxYU6=
MiaPaCa NXzB[ZNIS3m2b4TvfIlkcXS7IHHzd4F6 NV3z[4N2PzJiaILz NF7aN2pKSzVyIE2gNVcvOSEQvF2= NU\pOldyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFk3OjZpPkKzOFg6PjJ4PD;hQi=>
AG6000 MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEXjboNKSzVyIE2gNlAh|ryP NF7OZmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wNlQ3PCd-MUe2NFI1PjR:L3G+
AG6000 MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHzqTJJKSzVyIE2gNlAh|ryP MonSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
BxPC3 M4\6bGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MYi3NkBpenN? MoD0TWM2OCB;IEKwMlIh|ryP NFXqWYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
CV1 NH3rPWVEgXSxdH;4bYNqfHliYYPzZZk> NWfiSINQPzJiaILz NXTkdYNFUUN3MDC9JFIyNjlizszN NGPwUJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S4PVYzPid-MkO0PFk3OjZ:L3G+
AsPC1 MXHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2jjblczKGi{cx?= M2PET2lEPTBiPTCyOk45KM7:TR?= NFnzVG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
PANC1 NIjT[4dCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWK3NkBpenN? NVTOWlZuUUN3MDC9JFMxNjRizszN MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV5Nk[zN{c,Ojh3N{[2N|M9N2F-
MIAPaCa2 M4jYOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHn5[JM1QCCqcoO= MV\HTVUxKD1iM{WuPFMh|ryP NIT5cI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-JNK / JNK / p-ATF2 / p-c-Jun; 

PubMed: 16307741     


Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h.

caspase-3 / caspase-8 / caspase-9; 

PubMed: 16307741     


H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control.

pS-ERα / ERα / pERK / ERK; 

PubMed: 27384485     


MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected.

LC3-I / LC3-II; 

PubMed: 27384485     


A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h).

p70S6K1 / p-S6 / HIF-1α; 

PubMed: 27765914     


Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control. 

16307741 27384485 27765914
Immunofluorescence
BMI1; 

PubMed: 27177084     


Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining. 

27177084
Growth inhibition assay
Cell viability ; 

PubMed: 27765914     


A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.

27765914
In vivo Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. [2]

Protocol

Cell Research:[2]
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  • Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
  • Concentrations: 0.2 μM
  • Incubation Time: 24 hours or 48 hours
  • Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Athymic nude mice with MIA PaCa-2 cells
  • Dosages: 50 mg/kg or 100 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 19 mg/mL (63.4 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
19mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 299.66
Formula

C9H11F2N3O4.HCI

CAS No. 122111-03-9
Storage powder
in solvent
Synonyms NSC 613327
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04871321 Recruiting Drug: nab-paclitaxel plus gemcitabine-cisplatin Biliary Tract Cancer|Cholangiocarcinoma|Gallbladder Cancer CHA University|Dong-A Pharmaceutical July 1 2021 --
NCT04983407 Recruiting Drug: AVB-S6-500|Drug: Nab paclitaxel|Drug: Gemcitabine Pancreatic Adenocarcinoma Aravive Inc. July 28 2021 Phase 1|Phase 2
NCT04896320 Not yet recruiting Drug: Tucatinib Breast Cancer Stage IV Providence Health & Services|Seagen Inc. July 2021 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    What’s the difference between S1714 and S1149 and which one is better?

  • Answer:

    They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

DNA/RNA Synthesis Signaling Pathway Map

Related DNA/RNA Synthesis Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID