Gemcitabine (LY-188011) HCl

Name: Gemcitabine (LY-188011) HCl
Brand: Selleck Chemicals
SKU: S1149
Rating: 5 (107 reviews)

For research use only.

Catalog No.S1149 Synonyms: NSC 613327

107 publications

Gemcitabine (LY-188011) HCl Chemical Structure

CAS No. 122111-03-9

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Selleck's Gemcitabine (LY-188011) HCl has been cited by 107 publications

Cancer Cell, 2019, 35(3):519-533

PubMed: 30889383 ( click the link to review the publication )

Nat Commun, 2021, 12(1):1602

PubMed: 33707442 ( click the link to review the publication )

Oncogene, 2021, 10.1038/s41388-020-01634-z

PubMed: 33452459 ( click the link to review the publication )

Cell Death Dis, 2021, 12(1):72

PubMed: 33436545 ( click the link to review the publication )

Neoplasia, 2021, 23(7):643-652

PubMed: 34126361 ( click the link to review the publication )

Oxid Med Cell Longev, 2021, 2021:6673828

PubMed: 34055197 ( click the link to review the publication )

Sci Rep, 2021, 11(1):10181

PubMed: 33986399 ( click the link to review the publication )

Invest New Drugs, 2021, 10.1007/s10637-020-01003-3

PubMed: 33475937 ( click the link to review the publication )

J Pharm Biomed Anal, 2021, 198:114025

PubMed: 33744463 ( click the link to review the publication )

BMC Urol, 2021, 21(1):96

PubMed: 34210300 ( click the link to review the publication )

Cancer Med, 2021, 10.1002/cam4.3739

PubMed: 33626233 ( click the link to review the publication )

Cancers (Basel), 2021, 13(3)527

PubMed: 33573176 ( click the link to review the publication )

Nat Commun, 2020, 11(1):3018

PubMed: 32541668 ( click the link to review the publication )

Nat Commun, 2020, 12;11(1):2971

PubMed: 32532990 ( click the link to review the publication )

Nat Commun, 2020, 10;11(1):2935

PubMed: 32523045 ( click the link to review the publication )

Nat Commun, 2020, 14;11(1):1825

PubMed: 32286280 ( click the link to review the publication )

Nat Commun, 2020, 24;11(1):1975

PubMed: 32332851 ( click the link to review the publication )

Clin Cancer Res, 2020, 27 pii: clincanres

PubMed: 32341033 ( click the link to review the publication )

Mol Syst Biol, 2020, 16(2):e8664

PubMed: 32073727 ( click the link to review the publication )

Oncogene, 2020, 10.1038/s41388-020-1364-7

PubMed: 32561852 ( click the link to review the publication )

Cancers (Basel), 2020, 12(12)E3816

PubMed: 33348809 ( click the link to review the publication )

Cancers (Basel), 2020, 15;12(4) pii: E974

PubMed: 32326402 ( click the link to review the publication )

Neoplasia, 2020, 22(1):22-32

PubMed: 31765939 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(10):3302-3315

PubMed: 33163271 ( click the link to review the publication )
Int J Mol Sci, 2020, 21(23)E9034

PubMed: 33261142 ( click the link to review the publication )

Sci Rep, 2020, 10(1):18489

PubMed: 33116269 ( click the link to review the publication )

Sci Rep, 2020, 15;10(1):337

PubMed: 31941932 ( click the link to review the publication )

J Biol Chem, 2020, 1 pii: jbc

PubMed: 32358063 ( click the link to review the publication )

Food Chem Toxicol, 2020, 147:111919

PubMed: 33301843 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):984

PubMed: 33046037 ( click the link to review the publication )

Cancer Res Treat, 2020, 10.4143/crt.2020.585

PubMed: 33070556 ( click the link to review the publication )

Med Sci Monit, 2020, 26:e922703

PubMed: 32946432 ( click the link to review the publication )

Anticancer Res, 2020, 40(7):3659-3667

PubMed: 32620605 ( click the link to review the publication )

J Biochem Mol Toxicol, 2020, e22621

PubMed: 32894642 ( click the link to review the publication )

Mediators Inflamm, 2020, 2020:8567320

PubMed: 32684837 ( click the link to review the publication )

Genes Chromosomes Cancer, 2020, 10.1002/gcc.22884

PubMed: 32614991 ( click the link to review the publication )

Am J Transl Res, 2020, 12(6):2827-2842

PubMed: 32655813 ( click the link to review the publication )

bioRxiv, 2020, 2020/9/20.4.7.30734

PubMed: 32511305 ( click the link to review the publication )

J Immunother Cancer, 2019, 7(1):199

PubMed: 31362778 ( click the link to review the publication )

J Control Release, 2019, 305:89-100

PubMed: 31096017 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E2042

PubMed: 31861153 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E1835

PubMed: 31766357 ( click the link to review the publication )

Cells, 2019, 8(8)

PubMed: 31390735 ( click the link to review the publication )

Cell Prolif, 2019, 52(1):e12514

PubMed: 30341797 ( click the link to review the publication )

Cancer Sci, 2019, 110(1):334-344

PubMed: 30353606 ( click the link to review the publication )

Int J Biol Sci, 2019, 15(9):1771-1786

PubMed: 31523182 ( click the link to review the publication )

Invest New Drugs, 2019, 10.1007/s10637-019-00839-8

PubMed: 31463638 ( click the link to review the publication )

BMC Cancer, 2019, 19(1):604

PubMed: 31216997 ( click the link to review the publication )
Biosci Rep, 2019, 10.1042/BSR20192358

PubMed: 31693728 ( click the link to review the publication )

Exp Ther Med, 2019, 18(1):550-558

PubMed: 31258693 ( click the link to review the publication )

Nanomedicine (Lond), 2019, 14(15):1971-1989

PubMed: 31355712 ( click the link to review the publication )

Nat Commun, 2018, 13;9(1):2720

PubMed: 30006524 ( click the link to review the publication )

Nat Commun, 2018, 9(1):2434

PubMed: 29934552 ( click the link to review the publication )

Cancer Res, 2018, 78(19):5656-5667

PubMed: 30217928 ( click the link to review the publication )

Oncogene, 2018, 37(44):5843-5857

PubMed: 29980787 ( click the link to review the publication )

Sci Signal, 2018, 11(540)eaat0229

PubMed: 30042127 ( click the link to review the publication )

Cancers (Basel), 2018, 10(5)

PubMed: 29783721 ( click the link to review the publication )

Mol Ther Nucleic Acids, 2018, 13:472-482

PubMed: 30388621 ( click the link to review the publication )

Cell Chem Biol, 2018, 25(6):705-717

PubMed: 29628435 ( click the link to review the publication )

J Adv Res, 2018, 15:59-68

PubMed: 30581613 ( click the link to review the publication )

Neoplasia, 2018, 20(8):826-837

PubMed: 30015158 ( click the link to review the publication )

Oxid Med Cell Longev, 2018, 2018:9482018

PubMed: 29765509 ( click the link to review the publication )

Oxid Med Cell Longev, 2018, 2018:2360427

PubMed: 29849873 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(10)

PubMed: 30274301 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(2)

PubMed: 29462944 ( click the link to review the publication )

J Biol Chem, 2018, 293(3):984-994

PubMed: 29191829 ( click the link to review the publication )

Apoptosis, 2018, 23(5-6):343-355

PubMed: 29740790 ( click the link to review the publication )

Pharm Res, 2018, 35(8):154

PubMed: 29855807 ( click the link to review the publication )

Dig Dis Sci, 2018, 63(12):3367-3375

PubMed: 30155839 ( click the link to review the publication )

Cancer Biomark, 2018, 10.3233/CBM-181182

PubMed: 29526843 ( click the link to review the publication )

Anticancer Res, 2018, 38(4):1967-1977

PubMed: 29599312 ( click the link to review the publication )

Oncol Lett, 2018, 15(5):6107-6114

PubMed: 29731842 ( click the link to review the publication )
Cancer Lett, 2017, 411:106-116

PubMed: 28947137 ( click the link to review the publication )

Cell Death Dis, 2017, 8(10):e3129

PubMed: 29048402 ( click the link to review the publication )

Cell Death Dis, 2017, 8(5):e2770

PubMed: 28492560 ( click the link to review the publication )

Br J Cancer, 2017, 116(3):324-334

PubMed: 28056464 ( click the link to review the publication )

Mol Oncol, 2017, 11(9):1130-1142

PubMed: 28544747 ( click the link to review the publication )

FEBS J, 2017, 284(2):222-236

PubMed: 27860276 ( click the link to review the publication )

J Cell Mol Med, 2017, 21(9):2055-2067

PubMed: 28244691 ( click the link to review the publication )

PLoS One, 2017, 12(11):e0188859

PubMed: 29186204 ( click the link to review the publication )

Acta Biochim Biophys Sin (Shanghai), 2017, 49(7):573-580

PubMed: 28475672 ( click the link to review the publication )

Oncotarget, 2017, 8(35):58417-58429

PubMed: 28938567 ( click the link to review the publication )

J Pineal Res, 2016, 60(1):27-38

PubMed: 26445000 ( click the link to review the publication )

Cancer Res, 2016, 76(13):3978-88

PubMed: 27216178 ( click the link to review the publication )

Cancer Lett, 2016, 380(2):434-41

PubMed: 27400681 ( click the link to review the publication )

Antiviral Res, 2016, 126:69-80

PubMed: 26738783 ( click the link to review the publication )

Am J Pathol, 2016, 186(6):1537-46

PubMed: 27102771 ( click the link to review the publication )

PLoS One, 2016, 11(6):e0158617

PubMed: 27359113 ( click the link to review the publication )

Tumour Biol, 2016, 37(9):12731-12742

PubMed: 27448305 ( click the link to review the publication )

Sci Transl Med, 2015, 7(284):284ra57

PubMed: 25904741 ( click the link to review the publication )

Mol Cancer Ther, 2015,

PubMed: 25344582 ( click the link to review the publication )

Cancer Sci, 2015, 106(1):9-17

PubMed: 25363339 ( click the link to review the publication )

Biomedicine & Pharmacotherapy, 2015, 73:123-8

PubMed: 26211592 ( click the link to review the publication )

Eur J Pharmacol, 2015, 10.1016/j.ejphar.2015.03.064

PubMed: 25843411 ( click the link to review the publication )

PLoS One, 2015, 10(4):e0123901

PubMed: 25875914 ( click the link to review the publication )

Anticancer Drugs, 2015, 26(6):612-9

PubMed: 25734833 ( click the link to review the publication )
Nucleic Acids Res, 2014, 42(10):6436-47

PubMed: 24728989 ( click the link to review the publication )

Clin Cancer Res, 2014, 20(12):3187-97

PubMed: 24838526 ( click the link to review the publication )

J Immunol, 2014, 192(9):4273-83

PubMed: 24670807 ( click the link to review the publication )

Mol Pharmacol, 2014, 85(3):408-19

PubMed: 24353313 ( click the link to review the publication )

Pancreas, 2014, 44(1):144-151

PubMed: 25237909 ( click the link to review the publication )

Freien Universit, 2014, Christian K?hler

PubMed: ( click the link to review the publication )

Cell Death Dis, 2013, 4:e742

PubMed: 23887633 ( click the link to review the publication )

Cancer Immunol Immun, 2013, 62(2):383-91

PubMed: 22926062 ( click the link to review the publication )

Biochemistry, 2013, 52(40):7050-9

PubMed: 24024562 ( click the link to review the publication )

Sci Signal, 2011, 1(4):352-65

PubMed: 22145099 ( click the link to review the publication )

Pancreas, 2011, 40(5):730-9

PubMed: 21633318 ( click the link to review the publication )

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Features

Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.

Targets

DNA synthesis (Capan2 cells) ^[1]	DNA synthesis (BxPC3 cells) ^[1]	DNA synthesis (MIAPaCa2 cells) ^[1]	DNA synthesis (PANC1 cells) ^[1]
12 nM	18 nM	40 nM	50 nM

In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
CCRF-CEM	M1HmRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M4j6cmlEPTB;Mj65JOKyKDFwODDuUS=>	M1WyOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM/dCK−/−	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NUjmNlNsUUN3ME2yOFAvPCEEsTCyPU4xKM7:TR?=	NYTpfHA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 wt	NX;udYE4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NX7FdYN4UUN3ME2xMlMhyrFiMD6zJI5O	NGjaOFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
L1210 10K	NGfTTpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NWrqPYJvUUN3ME2yNk4zKMLzIEOuO{DPxE1?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
TC-1	NYX1ZWV5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MlnSTWM2OD1zND63JOKyKDJwODDuUS=>	M1LEdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
TC-1-GR	M2nVWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NXm3bm1iUUN3ME2zOk44KMLzIEWuNUDPxE1?	NEjaSnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
MIA PaCa-2	NGjyfWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		M2\Q[mlEPTB;NEmuO{DDuSBzNz63JI5O	M2LqXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
PANC-1	M2XhVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NFj3VnJKSzVyPU6gOFAxKM7:TR?=	M2H0U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM	NEfHUJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NXu3ZpFIUUN3ME2yMlkhyrFiMT64JI5O	NFXyc5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM-AraC-8C	NV[yZWF7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NH;1U3FKSzVyPUm5PE45KMLzIEmuOEBvVQ>?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM	M{mw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXXRb4UzPzJiaB?=	NF;4[lNKSzVyPUKuNEDDuSByLk[gcm0>	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUi0N{c,OjF6NUG4OFM9N2F-
MV-4-11	NIfq[2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NEL0SJpKSzVyIE2gNE4xODB3IH7N	NWT3RWp[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ES4	M2K1UWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NUfGeWtwUUN3MDC9JFAvODByNzDuUS=>	M2jXbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ACHN	NHPMbGdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MlToTWM2OCB;IECuNFAxQSCwTR?=	M4LNPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
KYSE-510	M3vhOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NXfJOoh2UUN3MDC9JFAvODBzIH7N	Mom5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
EW-7	NUnUN\|JqT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MkjWTWM2OCB;IECuNFAzPiCwTR?=	NGjUVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
BFTC-905	NH\XRpFIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M1\KPWlEPTBiPTCwMlAxPTFibl2=	MojGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
KE-37	M3jvOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NX\mPZdqUUN3MDC9JFAvODB3NjDuUS=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SBC-5	Mn3zS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MmX4TWM2OCB;IECuNFA2PyCwTR?=	NFj6dI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NKM-1	M1Lyd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M{nSSGlEPTBiPTCwMlAxPzFibl2=	M{m3V\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
RH-1	M1;hN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M3GwZmlEPTBiPTCwMlAxPzJibl2=	MnTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ALL-PO	MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MoTITWM2OCB;IECuNFA5OyCwTR?=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
QIMR-WIL	NHvQdGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NHzNNYtKSzVyIE2gNE4xODh7IH7N	NHzWdmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375	NWSzXWdyT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MkHqTWM2OCB;IECuNFA6QSCwTR?=	MliyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SIG-M5	NFHtV4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MmrUTWM2OCB;IECuNFExPCCwTR?=	NHLCe\|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KGN	NVTlR2p7T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MlvqTWM2OCB;IECuNFExQCCwTR?=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-13	MljoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MnvxTWM2OCB;IECuNFEyOiCwTR?=	NHj6Zmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NCI-SNU-1	NEHJNFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NUDheI44UUN3MDC9JFAvODF4IH7N	NXvoTHdPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
PSN1	MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M2X5XmlEPTBiPTCwMlAyPjVibl2=	NXLJcIpIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
HUTU-80	MnvkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NEHpfVlKSzVyIE2gNE4xOTZ4IH7N	NVPXZYlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
786-0	MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M1XJbmlEPTBiPTCwMlAzOyCwTR?=	M1HOTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
EW-16	MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MWTJR\|UxKD1iMD6wNlMhdk1?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ES1	NFrpd4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MYjJR\|UxKD1iMD6wNlY5KG6P	M122[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
RKO	NGCxSlhIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MUXJR\|UxKD1iMD6wNlc5KG6P	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ESS-1	MkLuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M4L5fmlEPTBiPTCwMlAzQDZibl2=	NW\rOYdtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
SK-UT-1	MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MYfJR\|UxKD1iMD6wNlk4KG6P	NYLpeI9xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
LB2241-RCC	M2TmRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M{nnPWlEPTBiPTCwMlA{OThibl2=	NH72WnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
CHL-1	MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MoO3TWM2OCB;IECuNFMzPCCwTR?=	MoPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SW1783	NHPhN4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		Mlu1TWM2OCB;IECuNFM{PiCwTR?=	MkjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
MEL-JUSO	NFrpU2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MXfJR\|UxKD1iMD6wN\|kyKG6P	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HT-29	M2P2V2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NHz3NllKSzVyIE2gNE4xPDF\|IH7N	MnHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SNG-M	MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M3zGUGlEPTBiPTCwMlA1OjVibl2=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
TE-15	MlLpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M4XEW2lEPTBiPTCwMlA1PjRibl2=	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HOS	MmH2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M1z0PWlEPTBiPTCwMlA1QCCwTR?=	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
BB65-RCC	NUnGUnlRT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MkjVTWM2OCB;IECuNFUyOiCwTR?=	M3nZdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HCE-4	MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MnviTWM2OCB;IECuNFUzQCCwTR?=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
MHH-ES-1	M1izPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NX3yZXg3UUN3MDC9JFAvODV\|MTDuUS=>	NGq3dIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
RPMI-7951	NVjDTWQ{T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MXjJR\|UxKD1iMD6wOVQyKG6P	M3PnTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
IST-SL2	NULW[21DT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NFrkeFFKSzVyIE2gNE4xPTh2IH7N	NGO2bHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
CMK	MkTxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M135T2lEPTBiPTCwMlA2QDZibl2=	NYrQW\|VxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
GR-ST	NVTvV4tYT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M{LQO2lEPTBiPTCwMlA2QTVibl2=	NX\zdo9XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NALM-6	NGLjflZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NWPOcYJjUUN3MDC9JFAvODZ{MjDuUS=>	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
RPMI-6666	Mn:0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MYrJR\|UxKD1iMD6wOlUzKG6P	NFXTO3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
LC-2-ad	MoqyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MorHTWM2OCB;IECuNFY2OyCwTR?=	Ml3UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ARH-77	MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MUDJR\|UxKD1iMD6wO\|EyKG6P	NHf6cXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
IST-MEL1	MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NFPYSY1KSzVyIE2gNE4xPzJ4IH7N	NYC0TG5oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
SW1710	NVzkTmROT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NH25N5JKSzVyIE2gNE4xPzVzIH7N	NWe1cYp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
DEL	NX;jR3BWT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NX;qUItFUUN3MDC9JFAvODh6NzDuUS=>	M{TCOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
AGS	MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NV;MVIhGUUN3MDC9JFAvODlyMjDuUS=>	NXvzV\|M3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NCI-H2122	Mo\1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MVPJR\|UxKD1iMD6wPVQ2KG6P	MnP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
HSC-4	M{LGVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MmD6TWM2OCB;IECuNVAzOiCwTR?=	MnqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
AM-38	NV7sVJJST3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NXm5PVBSUUN3MDC9JFAvOTJzNTDuUS=>	NXezTVE2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
769-P	MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NUDkWFdOUUN3MDC9JFAvOTJ\|MTDuUS=>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
RT-112	MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MlKzTWM2OCB;IECuNVI4OyCwTR?=	NXL1bYxiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
MCF7	NGfMfFFIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NVP4TGRUUUN3MDC9JFAvOTN3OTDuUS=>	NFiwNm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
IGROV-1	MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NYPwSohPUUN3MDC9JFAvOTR3IH7N	MmriQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
OCI-AML2	NYXOTXN6T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M3[0OmlEPTBiPTCwMlE1PjZibl2=	NFu4V449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NCI-H1299	M{\ucWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NU\vNW5NUUN3MDC9JFAvOTV4NjDuUS=>	MmHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
A431	MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NU\nZVJ1UUN3MDC9JFAvOTh\|MTDuUS=>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SW982	MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NIq0TXlKSzVyIE2gNE4zOTN\|IH7N	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
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L1210	Mo\6R5l1d3SxeHnjbZR6KGG\|c3H5	NUH0T2tjOiCmYYnz	NGrVUIZKSzVyIE2gNE4xOTNizszN	Mn7xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NEGzOVYoRjJ2M{SxN\|U3RC:jPh?=
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SW480	Mn;MRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	MUS3NkBpenN?	NHrORVRKSzVyIE2gNE4xOTN4IN88US=>	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR4OUiwPUc,OTh2Nkm4NFk9N2F-
A549	MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MWnJR\|UxKD1iMD6wNVQh\|ryP	MljRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
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SW1573	Mn;tS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		Mn;ITWM2OCB;IECuNFE3KM7:TR?=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
A2780	NULjbmZOSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	NYjufZlbPzJiaILz	MVPJR\|UxKD1iMD6wNVY3KM7:TR?=	NGnXeGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES2PVgxQSd-MUi0Olk5ODl:L3G+
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A549	NHjDemFEgXSxdH;4bYNqfHliYYPzZZk>	MVmyOEBpenN?	MYrJR\|UxKD1iMD6wNlkh\|ryP	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh5NEOzNEc,OjV6N{SzN\|A9N2F-
HT29	M2TheWFvfGm2dX3vdkBie3OjeR?=	MUK0PEBpenN?	MUnHTVUxKD1iMD6wN{DPxE1?	NV20fJZ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1PFgzQDFpPkG4OVg5OjhzPD;hQi=>
Jurkat	MkPmRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	M2mwSFczKGi{cx?=	NEnBbVBKSzVyIE2gNE4xOyEQvF2=	NHizV5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N\|c1OCd-MkC4O\|M4PDB:L3G+
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MDA-MB-231	MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NGPxdos4OiCqcoO=	NFHIOXNKSzVyIE2gNE4xOyEQvF2=	M4D5WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkWzN\|QxLz5{OUK1N\|M1ODxxYU6=
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DU-145	MU\DfZRwfG:6aXPpeJkh[XO\|YYm=		MU\JR\|UxKD1iMD6wN\|U3KM7:TR?=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd{OEG5NUc,OTF5MkixPVE9N2F-
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K562	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=	M{\HTVQ5KGi{cx?=	M33sSWlEPTBiPTCwMlA2KM7:TR?=	M4jxblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkOxN\|U6Lz5{NE[zNVM2QTxxYU6=
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K562	MXnDfZRwfG:6aXPpeJkh[XO\|YYm=	M4LuTVczKGi{cx?=	MX\JR\|UxKD1iMD6xJO69VQ>?	MmjMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{MkG3PVAoRjJ6MkKxO\|kxRC:jPh?=
CEM-DNR-bulk	MV7DfZRwfG:6aXPpeJkh[XO\|YYm=	MmT2O\|IhcHK\|	MnPwTWM2OCB;IECuNUDPxE1?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ{MUe5NEc,Ojh{MkG3PVA9N2F-
MIAPaCa2	MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	M4jBfFQ5KGi{cx?=	MUXMR\|UxKD1iMD6xJO69VQ>?	M2m1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEm0PVkzLz5{M{C5OFk6OjxxYU6=
H460	M2XmUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		Mn7CTWM2OCB;IECuNVA{KM7:TR?=	NYfhb4U3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
RT112	M4PPPWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NUPiSnk5TUN3MDC9JFAvOTB2OTFOwG0>	NWnTXXcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|E6QThpPkK0OFcyQTl6PD;hQi=>
PANC1	MV;DfZRwfG:6aXPpeJkh[XO\|YYm=	MmPBOFghcHK\|	MWjJR\|UxKD1iMD6xNUDPxE1?	NV\5cFRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOFIyPDZpPkKyN\|QzOTR4PD;hQi=>
MIAPaCa2	M3jVXGN6fG:2b4jpZ4l1gSCjc4PhfS=>	M{PNcVczKGi{cx?=	MYPJR\|UxKD1iMD6xNUDPxE1?	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN{OE[1Okc,Ojl\|Mki2OVY9N2F-
MIAPaCa2	MYDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	M4TjS\|cxKGi{cx?=	MW\JR\|UxKD1iMD6xNkDPxE1?	M{njS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEexNVE6Lz5{OUS3NVEyQTxxYU6=
CEM	NXjOXYVXT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MkPUTWM2OCB;IECuNVMh\|ryP	NYPucZZSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
MCF7	NFPzTWNEgXSxdH;4bYNqfHliYYPzZZk>	M33UZlMh\GG7cx?=	M2DEUGlEPTBiPTCwMlE1QSEQvF2=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
MCF7	MV7DfZRwfG:6aXPpeJkh[XO\|YYm=	Mn;rO\|IhcHK\|	NWnoXnJMUUN3MDC9JFAvOTVizszN	MmnYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMkW4O\|UoRjJ4MEK1PFc2RC:jPh?=
CaCo2	NIHSRXFCdnSrdIXtc5Ih[XO\|YYm=	NG\JU2E1QCCqcoO=	M3nZOGdKPTBiPTCwMlE5KM7:TR?=	NIjKbYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4PFI5OSd-MUi1PFgzQDF:L3G+
U2OS	M{DWeWFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NHvwNHg4OiCqcoO=	MUTJR\|UxKD1iMD6xPEDPxE1?	MmnhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
U2OS	NE\sTFBEgXSxdH;4bYNqfHliYYPzZZk>	NFfIOJY4OiCqcoO=	M4LXeWlEPTBiPTCwMlE5KM7:TR?=	NXPVUoZURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyNlE4QTBpPkK4NlIyPzlyPD;hQi=>
MDA-MB-231	MlPpR5l1d3SxeHnjbZR6KGG\|c3H5	MmjmNlQhcHK\|	NF73d21KSzVyIE2gNE4yQSEQvF2=	MoTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEOyPVYoRjJ3N{CzNlk3RC:jPh?=
H292	NFT2[JJIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NFWzfmlKSzVyIE2gNE4zOSEQvF2=	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
A549	M1TsOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NHXnVlJKSzVyIE2gNE4zOjVizszN	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
NCI-H460	MljuR5l1d3SxeHnjbZR6KGG\|c3H5	MYW0PEBpenN?	M{P4UWlEPTBiPTCwMlI{KM7:TR?=	M3exNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{SyNVQ3Lz5{MkO0NlE1PjxxYU6=
MDA-MB-231	MV7DfZRwfG:6aXPpeJkh[XO\|YYm=	NWjtNZFsOyCmYYnz	M4TxSGlEPTBiPTCwMlI1PSEQvF2=	MlLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
SW1573	NXP1Nld4T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M33Y[GlEPTBiPTCwMlI4PSEQvF2=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
A2780	MYnDfZRwfG:6aXPpeJkh[XO\|YYm=	MmKwO\|IhcHK\|	MYjJR\|UxKD1iMD6zNUDPxE1?	NHzo[lg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
K562	NVK0c\|B5SW62aYT1cY9zKGG\|c3H5	NVT3TWl2PDhiaILz	MVTHTVUxKD1iMD6zNkDPxE1?	NFXt[pQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4PFI5OSd-MUi1PFgzQDF:L3G+
HCT116	NHfm[2hEgXSxdH;4bYNqfHliYYPzZZk>	NGLyUXY1QCCqcoO=	NHfKdlRKSzVyIE2gNE4{OiEQvF2=	MojUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|NEKxOFYoRjJ{M{SyNVQ3RC:jPh?=
CFPAC-1	MU\DfZRwfG:6aXPpeJkh[XO\|YYm=	MlXFPVYhcHK\|	MnrFTWM2OCB;IECuN\|Uh\|ryP	MljIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{GzOVkoRjJ2NkOxN\|U6RC:jPh?=
PANC1	MnfCR5l1d3SxeHnjbZR6KGG\|c3H5	NH;nV244OiCqcoO=	MWrJR\|UxKD1iMD60JO69VQ>?	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3NkKwNkc,Ojl4NU[yNFI9N2F-
HCT116	NW\wdGZ[S3m2b4TvfIlkcXS7IHHzd4F6	MWK3NkBpenN?	M3;RVGlEPTBiPTCwMlQyKM7:TR?=	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ{MUe5NEc,Ojh{MkG3PVA9N2F-
CFPAC-1	NIDI[o9EgXSxdH;4bYNqfHliYYPzZZk>	NYHrSHRVPzJiaILz	NF7HSFdKSzVyIE2gNE41PyEQvF2=	NYnBb4dsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N\|E{PTlpPkK0OlMyOzV7PD;hQi=>
ACHN	NGXOVmVEgXSxdH;4bYNqfHliYYPzZZk>	Mk\3OFghcHK\|	MYHJR\|UxKD1iMD60PEDPxE1?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2MkG0Okc,OjJ\|NEKxOFY9N2F-
HCT116-E6	NHnNUIFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	MWW3NkBpenN?	NUnkeWYxUUN3MDC9JFAvPSEQvF2=	MkLMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
C6	NXzmTpdzS3m2b4TvfIlkcXS7IHHzd4F6	MnG3N{Bl[Xm\|	NV\yU3l3UUN3MDC9JFAvPTB2IN88US=>	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
LNCAP	NVrwV2hOS3m2b4TvfIlkcXS7IHHzd4F6	MYKzJIRigXN?	NUXNTmhbUUN3MDC9JFAvPTF{IN88US=>	M33He\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
Calu1	NUPISXBkS3m2b4TvfIlkcXS7IHHzd4F6	M{G1Z\|Q5KGi{cx?=	MlfNTWM2OCB;IECuOVIh\|ryP	Ml;jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|NEKxOFYoRjJ{M{SyNVQ3RC:jPh?=
HT-29	NXmyPY5yS3m2b4TvfIlkcXS7IHHzd4F6	Ml7kO\|IhcHK\|	M3rGfGlEPTBiPTCwMlUzKM7:TR?=	MlS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMkW4O\|UoRjJ4MEK1PFc2RC:jPh?=
MCF7	M2HYV2N6fG:2b4jpZ4l1gSCjc4PhfS=>	NHPKW3Q4OiCqcoO=	M4rM[mlEPTBiPTCwMlU4KM7:TR?=	NWDMNGQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwO\|U2QTJpPkK4NFc2PTl{PD;hQi=>
K562	NWr2NnNESW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	NXjoV21rPzJiaILz	M4\STmlEPTBiPTCwMlYh\|ryP	MlTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
MIAPaCa2	M{nTe2N6fG:2b4jpZ4l1gSCjc4PhfS=>	MorSNlQhcHK\|	MULJR\|UxKD1iMD62JO69VQ>?	NESzc\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUewN\|I6Pid-MkW3NFMzQTZ:L3G+
MDA-MB-231	MYDDfZRwfG:6aXPpeJkh[XO\|YYm=	MnTDO\|IhcHK\|	M3\VeGlEPTBiPTCwMlY2KM7:TR?=	MlzyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMkW4O\|UoRjJ4MEK1PFc2RC:jPh?=
BxPC3	NIn5bG9EgXSxdH;4bYNqfHliYYPzZZk>	NIT3Roc4OiCqcoO=	NVHxO\|dXUUN3MDC9JFAvPjdizszN	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5MUm5PEc,OjR2N{G5PVg9N2F-
K562	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=	NYqxT41lOyCmYYnz	NFPGOHpKSzVyIE2gNE44OThizszN	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
K562	MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	MXO3NkBpenN?	MWLJR\|UxKD1iMD63OFU6KM7:TR?=	NF70fVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES2PVgxQSd-MUi0Olk5ODl:L3G+
Bel7402	MV7DfZRwfG:6aXPpeJkh[XO\|YYm=	NWHrc2JbPzJiaILz	MmDzTWM2OCB;IECuPFQh\|ryP	NI\J[Ys9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
HCT116/E6	NFToUY9CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	M2DVSlczKGi{cx?=	MWjJR\|UxKD1iMD64PVY2KM7:TR?=	M3jtfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NE[5PFA6Lz5zOES2PVgxQTxxYU6=
HeLa	NXS0[GlzS3m2b4TvfIlkcXS7IHHzd4F6	MYO5OkBpenN?	M4rNV2lEPTBiPTCwMlkh\|ryP	MnTZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{GzOVkoRjJ2NkOxN\|U6RC:jPh?=
CEM	NGDzfJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MXLJR\|UxKD1iMTFOwG0>	NVrjV5pXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
HB-1	MlnORY51cX[rcnHsJIF{e2G7	MWCyOEBpenN?	MVHFR\|UxKD1iMTFOwG0>	NWDBRXVQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1PFA2PTRpPkKwOVgxPTV2PD;hQi=>
MIAPaCa2	NWfsSpJQS3m2b4TvfIlkcXS7IHHzd4F6	NHy2R5I4OiCqcoO=	MmHQTWM2OCB;IEGuNFQh\|ryP	NW\IbnB6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|E6QThpPkK0OFcyQTl6PD;hQi=>
SK-N-AS	MYnDfZRwfG:6aXPpeJkh[XO\|YYm=	MWGzJIRigXN?	NWDBdYNGUUN3MDC9JFEvOSEQvF2=	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
SW1990	M2nMSWN6fG:2b4jpZ4l1gSCjc4PhfS=>	MUS3NkBpenN?	MXrJR\|UxKD1iMT6yJO69VQ>?	M{GzcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
A549	MYPDfZRwfG:6aXPpeJkh[XO\|YYm=	NULRPGhlPzJiaILz	NH3QTY5KSzVyIE2gNU41KM7:TR?=	MmP2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|NkK0O\|QoRjF7M{[yOFc1RC:jPh?=
HepG2	NWTuVldHTnWwY4Tpc44h[XO\|YYm=		MmfET40hRSBzLkSg{txO	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzFyMU[3OEc,OTdzMEG2O\|Q9N2F-
BL21(DE3)	M1jONGZ2dmO2aX;uJIF{e2G7		NFf3[otMdSB;IEGuOFMh\|ryP	Ml3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN\|EoRjJ\|MkOwNVMyRC:jPh?=
U87MG	NVPWVVAyS3m2b4TvfIlkcXS7IHHzd4F6	NWXQbVRwOyCmYYnz	MmPRTWM2OCB;IEGuOFkh\|ryP	M3zjNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
BL21(DE3)	M33jdmZ2dmO2aX;uJIF{e2G7		M3nIZWtuKD1iMT61NkDPxE1?	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ\|MEGzNUc,OjN{M{CxN\|E9N2F-
HT-29	MYnDfZRwfG:6aXPpeJkh[XO\|YYm=	Mlj1N{Bl[Xm\|	MX\JR\|UxKD1iMT61N{DPxE1?	M2H0cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990	NWmxfWtUS3m2b4TvfIlkcXS7IHHzd4F6		NFLCXVhKSzVyIE2gNU43KM7:TR?=	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF7NUS2Okc,OjRzOUW0OlY9N2F-
Capan2	MUPDfZRwfG:6aXPpeJkh[XO\|YYm=	Ml;sO\|IhcHK\|	MkS2TWM2OCB;IEGuO{DPxE1?	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl\|NkK0O\|Q9N2F-
SW480	Mnv2RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	MmHLO\|IhcHK\|	MYPJR\|UxKD1iMT63JO69VQ>?	MkPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
PANC1	M4PDPGN6fG:2b4jpZ4l1gSCjc4PhfS=>	M2POZ\|czKGi{cx?=	M372O2lEPTBiPTCxMlch\|ryP	MkPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4NU[yNFIoRjJ7NkW2NlAzRC:jPh?=
HCT8	NGXEV4pEgXSxdH;4bYNqfHliYYPzZZk>	NFrNO5c4OiCqcoO=	M3H2VWlEPTBiPTCxMlc1KM7:TR?=	NH33cWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
BL21(DE3)	NF:5[nFHfW6ldHnvckBie3OjeR?=		M1vPRWtuKD1iMT63OUDPxE1?	M3Xye\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkOwNVMyLz5{M{KzNFE{OTxxYU6=
HT-29	MUDDfZRwfG:6aXPpeJkh[XO\|YYm=	MlvQOFghcHK\|	MWnJR\|UxKD1iMT65OUDPxE1?	NEfnXpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MIAPaCa2	NIHSUFhEgXSxdH;4bYNqfHliYYPzZZk>	M3nJNlUh\GG7cx?=	MUfFR\|UxKD1iMjFOwG0>	NY\FT5h4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4Olc2QTBpPkK0PFY4PTlyPD;hQi=>
BL21(DE3)	NEXBO3FHfW6ldHnvckBie3OjeR?=	MlXKNUBucW5?	NGPOT\|lMdSB;IEKuNVUh\|ryP	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ\|MEGzNUc,OjN{M{CxN\|E9N2F-
SW1990	NEDUNZpEgXSxdH;4bYNqfHliYYPzZZk>		Ml7LTWM2OCB;IEKuNkDPxE1?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyNUeyNkc,OjVzMEW3NlI9N2F-
SW1990	NEPu[mdEgXSxdH;4bYNqfHliYYPzZZk>	NF\zWIM1QCCqcoO=	MUjJR\|UxKD1iMj6zJO69VQ>?	MlfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd7Nk[5OVAoRjJ5OU[2PVUxRC:jPh?=
NCI-H146	MkjDR5l1d3SxeHnjbZR6KGG\|c3H5	MXqzJIRigXN?	M1rPT2lEPTBiPTCyMlc5KM7:TR?=	M3jsXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
BxPC3	NYDrbZRGS3m2b4TvfIlkcXS7IHHzd4F6	M{G5WlczKGi{cx?=	NEjyTFBKSzVyIE2gNk46KM7:TR?=	M3fvV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
p53 deficient COLO205	M3r3ZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NGLrZYY4OiCqcoO=	NXzGO2RNUUN3MDC9JFMh\|ryP	NFXzeVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N\|c1OCd-MkC4O\|M4PDB:L3G+
MiaPaCa	NEnLSo9EgXSxdH;4bYNqfHliYYPzZZk>	MVe2JIRigXN?	NXroRXFbUUN3MDC9JFMh\|ryP	Ml:xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|NkCxNFQoRjJ\|M{[wNVA1RC:jPh?=
COLO205	MnXYR5l1d3SxeHnjbZR6KGG\|c3H5	MlGyOFghcHK\|	M33POmlEPTBiPTCzMlI1KM7:TR?=	M{PmeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OU[4PFI1Lz5{M{m2PFgzPDxxYU6=
MCF7	MnvxR5l1d3SxeHnjbZR6KGG\|c3H5	M4jNR\|czKGi{cx?=	MmTSTWM2OCB;IEOuNlgh\|ryP	NYjRcZZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N\|YzPDd2PD;hQi=>
MIAPaCa2	MXXDfZRwfG:6aXPpeJkh[XO\|YYm=	M3X0b\|czKGi{cx?=	Mn[yTWM2OCB;IEOuN{DPxE1?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN{OE[1Okc,Ojl\|Mki2OVY9N2F-
HeLa	NIXDOW1EgXSxdH;4bYNqfHliYYPzZZk>	MmnGNlQhcHK\|	MW\JR\|UxKD1iMz6zJO69VQ>?	Mo\HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEOyPVYoRjJ3N{CzNlk3RC:jPh?=
FTC-133	NVPKb2w5S3m2b4TvfIlkcXS7IHHzd4F6	M1v2fVI1KGi{cx?=	NUHzdGVMUUN3MDC9JFMvOzZizszN	MmrQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3	NICwdmpIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	M4TEXFQ5KGi{cx?=	M3\vO2dKPTBiPTCzMlY1KM7:TR?=	NXLUcIhKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NVI6ODhpPkKyOVEzQTB6PD;hQi=>
COLO320DM	MUjDfZRwfG:6aXPpeJkh[XO\|YYm=	M33nbFQ5KGi{cx?=	MknVTWM2OCB;IEOuPVIh\|ryP	NHjHXHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
HeLa	NFPhOXREgXSxdH;4bYNqfHliYYPzZZk>	NU\nVZo{OyCmYYnz	NXfHb5U1UUN3MDC9JFQvOTJizszN	NY[zflU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO\|EyODV2PD;hQi=>
8305C	NF7uZ2VEgXSxdH;4bYNqfHliYYPzZZk>	MmPXNlQhcHK\|	NETOTJpKSzVyIE2gOE42OyEQvF2=	NUfUSId{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N\|Y6QTRpPkK0OFM3QTl2PD;hQi=>
PANC1	MlXGR5l1d3SxeHnjbZR6KGG\|c3H5	M4PNRVczKGi{cx?=	NUj6VY94UUN3MDC9JFUvPiEQvF2=	M1PVVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
PANC1	NY\udYo3T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	MmizO\|IhcHK\|	MnSwS2k2OCB;IEWuPEDPxE1?	MoPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2OUWwPFEoRjJ6NEm1NFgyRC:jPh?=
SK-MEL-2	MUPDfZRwfG:6aXPpeJkh[XO\|YYm=	NHzFbms{KGSjeYO=	NXK1emJYUUN3MDC9JFcvOTFizszN	M2jXc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
CEM	MkLnR5l1d3SxeHnjbZR6KGG\|c3H5	NWLGWIsyOyCmYYnz	NHP6eWhKSzVyIE2gO{43KM7:TR?=	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN2MUO1Okc,OjR\|NEGzOVY9N2F-
SW1573	MmH4R5l1d3SxeHnjbZR6KGG\|c3H5	NIfLU3o4OiCqcoO=	NFGzXHRKSzVyIE2gPE4{KM7:TR?=	M{XRZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
BxPC3	NEjQWVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NF7QdmM1QCCqcoO=	NGjidYZNSzVyIE2gPE44OSEQvF2=	M4jxflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
MCF7	NYf0VJRlS3m2b4TvfIlkcXS7IHHzd4F6	Mmr1O\|IhcHK\|	MojJTWM2OCB;IEiuPUDPxE1?	NY\WVmlFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwO\|U2QTJpPkK4NFc2PTl{PD;hQi=>
BJ	NHi0VmVEgXSxdH;4bYNqfHliYYPzZZk>	MUCzJIRigXN?	M3H6T2lEPTBiPTC5Mlg5KM7:TR?=	M1rBWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
HeLa	NGnncWREgXSxdH;4bYNqfHliYYPzZZk>	NFHDbmg4OiCqcoO=	M1LGW2lEPTBiPTCxNEDPxE1?	NYPUe5F4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N\|E{PTlpPkK0OlMyOzV7PD;hQi=>
B16-F10-Luc-G5	M{W5OWFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NE\hS3UzPCCqcoO=	MWXJR\|UxKD1iMUGg{txO	NV7yb4c2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezOFk{OzJpPkK3N\|Q6OzN{PD;hQi=>
HCT15	NVvVPJp7S3m2b4TvfIlkcXS7IHHzd4F6		MkLmTWM2OCB;IEGxMlgh\|ryP	M1zxSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MUi2OlA1Lz5zOEG4OlYxPDxxYU6=
A549	MXfDfZRwfG:6aXPpeJkh[XO\|YYm=	MYO3NkBpenN?	MVjJR\|UxKD1iMUOuNUDPxE1?	M1P5W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
HCT116	MXrDfZRwfG:6aXPpeJkh[XO\|YYm=		Mkf5TWM2OCB;IEG0MlMh\|ryP	NFfZRnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG4OlYxPCd-MUixPFY3ODR:L3G+
MiaPaCa	Ml;CR5l1d3SxeHnjbZR6KGG\|c3H5	NW\ic3BQPzJiaILz	MYTJR\|UxKD1iMUeuNUDPxE1?	MkPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEm2NlYoRjJ\|NEi5OlI3RC:jPh?=
AG6000	MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NWT6cpVmUUN3MDC9JFIxKM7:TR?=	M2ThW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
AG6000	MoTIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NXTkeXJmUUN3MDC9JFIxKM7:TR?=	NITYd3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wNlQ3PCd-MUe2NFI1PjR:L3G+
BxPC3	MnzKRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	MYi3NkBpenN?	NXnzO4pGUUN3MDC9JFIxNjJizszN	NV7N[otuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O\|Y3OzNpPkK4OVc3PjN\|PD;hQi=>
CV1	MnLGR5l1d3SxeHnjbZR6KGG\|c3H5	MVW3NkBpenN?	NHXNfZlKSzVyIE2gNlEvQSEQvF2=	NVzj[JlkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFk3OjZpPkKzOFg6PjJ4PD;hQi=>
AsPC1	M1;NfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NIrxcIE4OiCqcoO=	NY\Ge5hyUUN3MDC9JFI3NjhizszN	NXfLUpFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O\|Y3OzNpPkK4OVc3PjN\|PD;hQi=>
PANC1	NXHLZm1DSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	NIOxcFY4OiCqcoO=	MYTJR\|UxKD1iM{CuOEDPxE1?	M1X1UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUe2OlM{Lz5{OEW3OlY{OzxxYU6=
MIAPaCa2	M{TLTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NWHnbVB[PDhiaILz	Mn7mS2k2OCB;IEO1Mlg{KM7:TR?=	NIS0eIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-JNK / JNK / p-ATF2 / p-c-Jun; PubMed: 16307741 FEBS Lett Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h. caspase-3 / caspase-8 / caspase-9; PubMed: 16307741 FEBS Lett H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control. pS-ERα / ERα / pERK / ERK; PubMed: 27384485 Oncotarget MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected. LC3-I / LC3-II; PubMed: 27384485 Oncotarget A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h). p70S6K1 / p-S6 / HIF-1α; PubMed: 27765914 Oncotarget Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control.	16307741 27384485 27765914
Immunofluorescence	BMI1; PubMed: 27177084 Oncotarget Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining.	27177084
Growth inhibition assay	Cell viability ; PubMed: 27765914 Oncotarget A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.	27765914

In vivo

Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. ^[2]

Protocol

Cell Research:^[2]

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Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
Concentrations: 0.2 μM
Incubation Time: 24 hours or 48 hours
Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
(Only for Reference)

Animal Research:^[2]

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Animal Models: Athymic nude mice with MIA PaCa-2 cells
Dosages: 50 mg/kg or 100 mg/kg
Administration: Administered via i.p.
(Only for Reference)

References

Solubility (25°C)

In vitro	Water	19 mg/mL (63.4 mM)
	DMSO	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): Saline For best results, use promptly after mixing.	19mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Gemcitabine (LY-188011) HCl SDF

Molecular Weight	299.66
Formula	C₉H₁₁F₂N₃O₄.HCI
CAS No.	122111-03-9
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NSC 613327
Smiles	C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-05-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04871321	Recruiting	Drug: nab-paclitaxel plus gemcitabine-cisplatin	Biliary Tract Cancer\|Cholangiocarcinoma\|Gallbladder Cancer	CHA University\|Dong-A Pharmaceutical	May 3 2021	--
NCT04634539	Recruiting	Drug: Gemcitabine\|Drug: Nab-paclitaxel\|Drug: L-glutamine	Advanced Pancreatic Adenocarcinoma\|Pancreatic Cancer\|Pancreatic Ductal Adenocarcinoma	Jun Gong MD\|Emmaus Medical Inc.\|Cedars-Sinai Medical Center	April 2021	Phase 1
NCT04616534	Recruiting	Drug: Elimusertib\|Drug: Gemcitabine	Advanced Fallopian Tube Carcinoma\|Advanced Malignant Solid Neoplasm\|Advanced Ovarian Carcinoma\|Advanced Pancreatic Adenocarcinoma\|Advanced Primary Peritoneal Carcinoma\|High Grade Ovarian Serous Adenocarcinoma\|Platinum-Resistant Fallopian Tube Carcinoma\|Platinum-Resistant Ovarian Carcinoma\|Platinum-Resistant Primary Peritoneal Carcinoma\|Stage II Pancreatic Cancer AJCC v8\|Stage IIA Pancreatic Cancer AJCC v8\|Stage IIB Pancreatic Cancer AJCC v8\|Stage III Fallopian Tube Cancer AJCC v8\|Stage III Ovarian Cancer AJCC v8\|Stage III Pancreatic Cancer AJCC v8\|Stage III Primary Peritoneal Cancer AJCC v8\|Stage IIIA Fallopian Tube Cancer AJCC v8\|Stage IIIA Ovarian Cancer AJCC v8\|Stage IIIA Primary Peritoneal Cancer AJCC v8\|Stage IIIA1 Fallopian Tube Cancer AJCC v8\|Stage IIIA1 Ovarian Cancer AJCC v8\|Stage IIIA2 Fallopian Tube Cancer AJCC v8\|Stage IIIA2 Ovarian Cancer AJCC v8\|Stage IIIB Fallopian Tube Cancer AJCC v8\|Stage IIIB Ovarian Cancer AJCC v8\|Stage IIIB Primary Peritoneal Cancer AJCC v8\|Stage IIIC Fallopian Tube Cancer AJCC v8\|Stage IIIC Ovarian Cancer AJCC v8\|Stage IIIC Primary Peritoneal Cancer AJCC v8\|Stage IV Fallopian Tube Cancer AJCC v8\|Stage IV Ovarian Cancer AJCC v8\|Stage IV Pancreatic Cancer AJCC v8\|Stage IV Primary Peritoneal Cancer AJCC v8\|Stage IVA Fallopian Tube Cancer AJCC v8\|Stage IVA Ovarian Cancer AJCC v8\|Stage IVA Primary Peritoneal Cancer AJCC v8\|Stage IVB Fallopian Tube Cancer AJCC v8\|Stage IVB Ovarian Cancer AJCC v8\|Stage IVB Primary Peritoneal Cancer AJCC v8	National Cancer Institute (NCI)	February 12 2021	Phase 1
NCT04657068	Recruiting	Drug: ART0380\|Drug: Gemcitabine	Advanced Cancer\|Metastatic Cancer\|Ovarian Cancer	Artios Pharma Ltd	January 13 2021	Phase 1\|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
What’s the difference between S1714 and S1149 and which one is better?
Answer:
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

DNA/RNA Synthesis Signaling Pathway Map

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Gemcitabine (LY-188011) HCl