Gemcitabine HCl

Name: Gemcitabine HCl
Brand: Selleck Chemicals
SKU: S1149
Rating: 5 (96 reviews)

For research use only.

Catalog No.S1149 Synonyms: LY188011

96 publications

CAS No. 122111-03-9

Gemcitabine HCl (LY188011) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Selleck's Gemcitabine HCl has been cited by 96 publications

Cancer Cell, 2019, 35(3):519-533

PubMed: 30889383 ( click the link to review the publication )

Oncogene, 2021, 10.1038/s41388-020-01634-z

PubMed: 33452459 ( click the link to review the publication )

Cell Death Dis, 2021, 12(1):72

PubMed: 33436545 ( click the link to review the publication )

Invest New Drugs, 2021, 10.1007/s10637-020-01003-3

PubMed: 33475937 ( click the link to review the publication )

Cancers (Basel), 2021, 13(3)527

PubMed: 33573176 ( click the link to review the publication )

Cancer Med, 2021, 10.1002/cam4.3739

PubMed: 33626233 ( click the link to review the publication )

Nat Commun, 2020, 11(1):3018

PubMed: 32541668 ( click the link to review the publication )

Nat Commun, 2020, 24;11(1):1975

PubMed: 32332851 ( click the link to review the publication )

Nat Commun, 2020, 10;11(1):2935

PubMed: 32523045 ( click the link to review the publication )

Nat Commun, 2020, 12;11(1):2971

PubMed: 32532990 ( click the link to review the publication )

Nat Commun, 2020, 14;11(1):1825

PubMed: 32286280 ( click the link to review the publication )

Clin Cancer Res, 2020, 27 pii: clincanres

PubMed: 32341033 ( click the link to review the publication )

Mol Syst Biol, 2020, 16(2):e8664

PubMed: 32073727 ( click the link to review the publication )

Oncogene, 2020, 10.1038/s41388-020-1364-7

PubMed: 32561852 ( click the link to review the publication )

Cancers (Basel), 2020, 12(12)E3816

PubMed: 33348809 ( click the link to review the publication )

Cancers (Basel), 2020, 15;12(4) pii: E974

PubMed: 32326402 ( click the link to review the publication )

Neoplasia, 2020, 22(1):22-32

PubMed: 31765939 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(10):3302-3315

PubMed: 33163271 ( click the link to review the publication )

Int J Mol Sci, 2020, 21(23)E9034

PubMed: 33261142 ( click the link to review the publication )

Sci Rep, 2020, 15;10(1):337

PubMed: 31941932 ( click the link to review the publication )

Sci Rep, 2020, 10(1):18489

PubMed: 33116269 ( click the link to review the publication )

J Biol Chem, 2020, 1 pii: jbc

PubMed: 32358063 ( click the link to review the publication )

Food Chem Toxicol, 2020, 147:111919

PubMed: 33301843 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):984

PubMed: 33046037 ( click the link to review the publication )
Cancer Res Treat, 2020, 10.4143/crt.2020.585

PubMed: 33070556 ( click the link to review the publication )

Med Sci Monit, 2020, 26:e922703

PubMed: 32946432 ( click the link to review the publication )

Anticancer Res, 2020, 40(7):3659-3667

PubMed: 32620605 ( click the link to review the publication )

Mediators Inflamm, 2020, 2020:8567320

PubMed: 32684837 ( click the link to review the publication )

bioRxiv, 2020, 2020/9/20.4.7.30734

PubMed: 32511305 ( click the link to review the publication )

Genes Chromosomes Cancer, 2020, 10.1002/gcc.22884

PubMed: 32614991 ( click the link to review the publication )

Am J Transl Res, 2020, 12(6):2827-2842

PubMed: 32655813 ( click the link to review the publication )

J Biochem Mol Toxicol, 2020, e22621

PubMed: 32894642 ( click the link to review the publication )

J Immunother Cancer, 2019, 7(1):199

PubMed: 31362778 ( click the link to review the publication )

J Control Release, 2019, 305:89-100

PubMed: 31096017 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E1835

PubMed: 31766357 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E2042

PubMed: 31861153 ( click the link to review the publication )

Cells, 2019, 8(8)

PubMed: 31390735 ( click the link to review the publication )

Cell Prolif, 2019, 52(1):e12514

PubMed: 30341797 ( click the link to review the publication )

Cancer Sci, 2019, 110(1):334-344

PubMed: 30353606 ( click the link to review the publication )

Int J Biol Sci, 2019, 15(9):1771-1786

PubMed: 31523182 ( click the link to review the publication )

Invest New Drugs, 2019, 10.1007/s10637-019-00839-8

PubMed: 31463638 ( click the link to review the publication )

BMC Cancer, 2019, 19(1):604

PubMed: 31216997 ( click the link to review the publication )

Biosci Rep, 2019, 10.1042/BSR20192358

PubMed: 31693728 ( click the link to review the publication )

Exp Ther Med, 2019, 18(1):550-558

PubMed: 31258693 ( click the link to review the publication )

Nanomedicine (Lond), 2019, 14(15):1971-1989

PubMed: 31355712 ( click the link to review the publication )

Nat Commun, 2018, 13;9(1):2720

PubMed: 30006524 ( click the link to review the publication )

Nat Commun, 2018, 9(1):2434

PubMed: 29934552 ( click the link to review the publication )

Sci Signal, 2018, 11(540)eaat0229

PubMed: 30042127 ( click the link to review the publication )
Cancers (Basel), 2018, 10(5)

PubMed: 29783721 ( click the link to review the publication )

Mol Ther Nucleic Acids, 2018, 13:472-482

PubMed: 30388621 ( click the link to review the publication )

Cell Chem Biol, 2018, 25(6):705-717

PubMed: 29628435 ( click the link to review the publication )

J Adv Res, 2018, 15:59-68

PubMed: 30581613 ( click the link to review the publication )

Neoplasia, 2018, 20(8):826-837

PubMed: 30015158 ( click the link to review the publication )

Oxid Med Cell Longev, 2018, 2018:2360427

PubMed: 29849873 ( click the link to review the publication )

Oxid Med Cell Longev, 2018, 2018:9482018

PubMed: 29765509 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(2)

PubMed: 29462944 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(10)

PubMed: 30274301 ( click the link to review the publication )

Apoptosis, 2018, 23(5-6):343-355

PubMed: 29740790 ( click the link to review the publication )

Pharm Res, 2018, 35(8):154

PubMed: 29855807 ( click the link to review the publication )

Dig Dis Sci, 2018, 63(12):3367-3375

PubMed: 30155839 ( click the link to review the publication )

Cancer Biomark, 2018, 10.3233/CBM-181182

PubMed: 29526843 ( click the link to review the publication )

Anticancer Res, 2018, 38(4):1967-1977

PubMed: 29599312 ( click the link to review the publication )

Oncol Lett, 2018, 15(5):6107-6114

PubMed: 29731842 ( click the link to review the publication )

Cancer Lett, 2017, 411:106-116

PubMed: 28947137 ( click the link to review the publication )

Cell Death Dis, 2017, 8(10):e3129

PubMed: 29048402 ( click the link to review the publication )

Cell Death Dis, 2017, 8(5):e2770

PubMed: 28492560 ( click the link to review the publication )

Br J Cancer, 2017, 116(3):324-334

PubMed: 28056464 ( click the link to review the publication )

Mol Oncol, 2017, 11(9):1130-1142

PubMed: 28544747 ( click the link to review the publication )

FEBS J, 2017, 284(2):222-236

PubMed: 27860276 ( click the link to review the publication )

J Cell Mol Med, 2017, 21(9):2055-2067

PubMed: 28244691 ( click the link to review the publication )

PLoS One, 2017, 12(11):e0188859

PubMed: 29186204 ( click the link to review the publication )

Acta Biochim Biophys Sin (Shanghai), 2017, 49(7):573-580

PubMed: 28475672 ( click the link to review the publication )
Oncotarget, 2017, 8(35):58417-58429

PubMed: 28938567 ( click the link to review the publication )

J Pineal Res, 2016, 60(1):27-38

PubMed: 26445000 ( click the link to review the publication )

Cancer Lett, 2016, 380(2):434-41

PubMed: 27400681 ( click the link to review the publication )

Antiviral Res, 2016, 126:69-80

PubMed: 26738783 ( click the link to review the publication )

Am J Pathol, 2016, 186(6):1537-46

PubMed: 27102771 ( click the link to review the publication )

PLoS One, 2016, 11(6):e0158617

PubMed: 27359113 ( click the link to review the publication )

Tumour Biol, 2016, 37(9):12731-12742

PubMed: 27448305 ( click the link to review the publication )

Sci Transl Med, 2015, 7(284):284ra57

PubMed: 25904741 ( click the link to review the publication )

Mol Cancer Ther, 2015,

PubMed: 25344582 ( click the link to review the publication )

Cancer Sci, 2015, 106(1):9-17

PubMed: 25363339 ( click the link to review the publication )

Biomedicine & Pharmacotherapy, 2015, 73:123-8

PubMed: 26211592 ( click the link to review the publication )

Eur J Pharmacol, 2015, 10.1016/j.ejphar.2015.03.064

PubMed: 25843411 ( click the link to review the publication )

PLoS One, 2015, 10(4):e0123901

PubMed: 25875914 ( click the link to review the publication )

Anticancer Drugs, 2015, 26(6):612-9

PubMed: 25734833 ( click the link to review the publication )

Nucleic Acids Res, 2014, 42(10):6436-47

PubMed: 24728989 ( click the link to review the publication )

Clin Cancer Res, 2014, 20(12):3187-97

PubMed: 24838526 ( click the link to review the publication )

J Immunol, 2014, 192(9):4273-83

PubMed: 24670807 ( click the link to review the publication )

Mol Pharmacol, 2014, 85(3):408-19

PubMed: 24353313 ( click the link to review the publication )

Pancreas, 2014, 44(1):144-151

PubMed: 25237909 ( click the link to review the publication )

Freien Universit, 2014, Christian K?hler

PubMed: ( click the link to review the publication )

Cell Death Dis, 2013, 4:e742

PubMed: 23887633 ( click the link to review the publication )

Cancer Immunol Immun, 2013, 62(2):383-91

PubMed: 22926062 ( click the link to review the publication )

Sci Signal, 2011, 1(4):352-65

PubMed: 22145099 ( click the link to review the publication )

Pancreas, 2011, 40(5):730-9

PubMed: 21633318 ( click the link to review the publication )

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description

Gemcitabine HCl (LY188011) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Features

Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.

Targets

DNA synthesis (Capan2 cells) ^[1]	DNA synthesis (BxPC3 cells) ^[1]	DNA synthesis (MIAPaCa2 cells) ^[1]	DNA synthesis (PANC1 cells) ^[1]
12 nM	18 nM	40 nM	50 nM

In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
CCRF-CEM	M3r0U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NVLJWWNMUUN3ME2yMlkhyrFiMT64JI5O	NIfUPIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM/dCK−/−	M4nCXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NF\1OGdKSzVyPUK0NE41KMLzIEK5MlAh\|ryP	NUXnfYtFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 wt	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MYjJR\|UxRTFwMzFCtUAxNjNibl2=	M2XwNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 10K	NVnyPGI2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NYTW[HpSUUN3ME2yNk4zKMLzIEOuO{DPxE1?	M2nQWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
TC-1	M3zvNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MU\JR\|UxRTF2LkegxtEhOi56IH7N	NFPQemw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
TC-1-GR	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NUHWTpNmUUN3ME2zOk44KMLzIEWuNUDPxE1?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
MIA PaCa-2	NEexOZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NFPEPGVKSzVyPUS5MlchyrFiMUeuO{BvVQ>?	NHnufmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
PANC-1	NUnEN4ZJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M13mT2lEPTB;PjC0NFAh\|ryP	NFPteZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NHPqVm9KSzVyPUKuPUDDuSBzLkigcm0>	NETHS289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM-AraC-8C	MmDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MYrJR\|UxRTl7OD64JOKyKDlwNDDuUS=>	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM	NEnte4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3TLXFczKGh?	NFjJWpRKSzVyPUKuNEDDuSByLk[gcm0>	MkD1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NUG4OFMoRjJzOEWxPFQ{RC:jPh?=
MV-4-11	M{nxPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MmLwTWM2OCB;IECuNFAxPSCwTR?=	NELp[ow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ES4	MnHRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M2nO[mlEPTBiPTCwMlAxODdibl2=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ACHN	NXfmXYdYT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M3TWSmlEPTBiPTCwMlAxODlibl2=	NED5RXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KYSE-510	MkHDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MnLHTWM2OCB;IECuNFAyKG6P	NVrTVGV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-7	NXrvXGlnT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NWDvNlZGUUN3MDC9JFAvODB{NjDuUS=>	MlHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
BFTC-905	NWPMbm5kT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M3f4bWlEPTBiPTCwMlAxPTFibl2=	NYfiU\|NORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KE-37	M3zPPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NHjhTmRKSzVyIE2gNE4xODV4IH7N	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SBC-5	NEXQXYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NXflO4p2UUN3MDC9JFAvODB3NzDuUS=>	NVPyeWRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NKM-1	NFHaWWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MVnJR\|UxKD1iMD6wNFcyKG6P	NHHofok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
RH-1	NXHaWHZ1T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MkjQTWM2OCB;IECuNFA4OiCwTR?=	M1;1NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ALL-PO	NWHMU5pET3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MWPJR\|UxKD1iMD6wNFg{KG6P	NXjaVJpsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
QIMR-WIL	NXzyNWh3T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NH3WZYZKSzVyIE2gNE4xODh7IH7N	NGT6Nmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375	Mm\lS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MnjITWM2OCB;IECuNFA6QSCwTR?=	MoHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SIG-M5	NF;KZXlIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M4WydWlEPTBiPTCwMlAyODRibl2=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
KGN	M4S0emdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MkTUTWM2OCB;IECuNFExQCCwTR?=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-13	NFLEepZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NHfrXI5KSzVyIE2gNE4xOTF{IH7N	NX\Bb\|Y4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NCI-SNU-1	MnvJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NEXCeFlKSzVyIE2gNE4xOTZibl2=	MnPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
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BB65-RCC	MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MWHJR\|UxKD1iMD6wOVEzKG6P	NYLFXYNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
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RPMI-7951	NF30b4pIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MlznTWM2OCB;IECuNFU1OSCwTR?=	NEG4ZnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
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HSC-4	NHXjWWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MoHETWM2OCB;IECuNVAzOiCwTR?=	NWC1ZZBkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
AM-38	MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MlzhTWM2OCB;IECuNVIyPSCwTR?=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
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RT-112	MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NWjrZVZTUUN3MDC9JFAvOTJ5MzDuUS=>	NYTjZXBzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
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OCI-AML2	MmC3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NIPufFBKSzVyIE2gNE4yPDZ4IH7N	M2TCN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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BB30-HNC	NI\hPINIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		Mln3TWM2OCB;IECuNlMyOiCwTR?=	NHHteGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
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647-V	M3LBSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NV;ufWRLUUN3MDC9JFAvOjR6MTDuUS=>	M1HyZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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K562	MYrDfZRwfG:6aXPpeJkh[XO\|YYm=	NYfvNohiPzJiaILz	NYfLNJprUUN3MDC9JFAvOSEQvF2=	NEPKcoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKyNVc6OCd-MkiyNlE4QTB:L3G+
CEM-DNR-bulk	M1PhT2N6fG:2b4jpZ4l1gSCjc4PhfS=>	NFTHPZM4OiCqcoO=	M4\XUGlEPTBiPTCwMlEh\|ryP	NHzuVWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEKyNVc6OCd-MkiyNlE4QTB:L3G+
MIAPaCa2	M2LTOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MoPpOFghcHK\|	NX7xcZFkVEN3MDC9JFAvOSEQvF2=	MljaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyOUS5PVIoRjJ\|MEm0PVkzRC:jPh?=
H460	MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M4\SeGlEPTBiPTCwMlExOyEQvF2=	Mn20QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
RT112	Mn;mR5l1d3SxeHnjbZR6KGG\|c3H5		Ml[5SWM2OCB;IECuNVA1QSEQvF2=	MkLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{G5PVgoRjJ2NEexPVk5RC:jPh?=
PANC1	NITFNIVEgXSxdH;4bYNqfHliYYPzZZk>	M1n3XlQ5KGi{cx?=	NF;2Z4FKSzVyIE2gNE4yOSEQvF2=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN2MkG0Okc,OjJ\|NEKxOFY9N2F-
MIAPaCa2	M1v6cWN6fG:2b4jpZ4l1gSCjc4PhfS=>	MoDBO\|IhcHK\|	MnjmTWM2OCB;IECuNVEh\|ryP	M3TJWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{K4OlU3Lz5{OUOyPFY2PjxxYU6=
MIAPaCa2	NY\sSVgxSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	MYC3NEBpenN?	MVTJR\|UxKD1iMD6xNkDPxE1?	M1rtNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEexNVE6Lz5{OUS3NVEyQTxxYU6=
CEM	MlfZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NHHZSYJKSzVyIE2gNE4yOyEQvF2=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
MCF7	MWjDfZRwfG:6aXPpeJkh[XO\|YYm=	MlzSN{Bl[Xm\|	M4[zNmlEPTBiPTCwMlE1QSEQvF2=	NU[3SFh[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO\|EyODV2PD;hQi=>
MCF7	NGjvPHhEgXSxdH;4bYNqfHliYYPzZZk>	MWm3NkBpenN?	NEHId2xKSzVyIE2gNE4yPSEQvF2=	NETveYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCyOVg4PSd-Mk[wNlU5PzV:L3G+
CaCo2	NXe5[lhXSW62aYT1cY9zKGG\|c3H5	NHvyd4U1QCCqcoO=	MmXjS2k2OCB;IECuNVgh\|ryP	NGTobXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4PFI5OSd-MUi1PFgzQDF:L3G+
U2OS	M4\BeGFvfGmycn;sbYZmemG2aY\lJIF{e2G7	Mm\iO\|IhcHK\|	MkfKTWM2OCB;IECuNVgh\|ryP	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5M{e0NEc,OjB6N{O3OFA9N2F-
U2OS	Mmn4R5l1d3SxeHnjbZR6KGG\|c3H5	NUXvWHlKPzJiaILz	MnTXTWM2OCB;IECuNVgh\|ryP	M1W3fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkKxO\|kxLz5{OEKyNVc6ODxxYU6=
MDA-MB-231	Mn3QR5l1d3SxeHnjbZR6KGG\|c3H5	NYDx[XNZOjRiaILz	NVv0cnlEUUN3MDC9JFAvOTlizszN	NWfB[41ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NFMzQTZpPkK1O\|A{Ojl4PD;hQi=>
H292	NUO3OGhwT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M1L0O2lEPTBiPTCwMlIyKM7:TR?=	NFHk[mg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wNlQ3PCd-MUe2NFI1PjR:L3G+
A549	NEXLXm5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MX\JR\|UxKD1iMD6yNlUh\|ryP	MknYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
NCI-H460	NX;2SoxlS3m2b4TvfIlkcXS7IHHzd4F6	MVW0PEBpenN?	MUfJR\|UxKD1iMD6yN{DPxE1?	M1XZVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{SyNVQ3Lz5{MkO0NlE1PjxxYU6=
MDA-MB-231	MV7DfZRwfG:6aXPpeJkh[XO\|YYm=	MnLXN{Bl[Xm\|	MYXJR\|UxKD1iMD6yOFUh\|ryP	NFHhZoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
SW1573	MkX0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M37JemlEPTBiPTCwMlI4PSEQvF2=	NUPZeFByRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
A2780	NHj2NnhEgXSxdH;4bYNqfHliYYPzZZk>	M2DWSlczKGi{cx?=	MYrJR\|UxKD1iMD6zNUDPxE1?	NFHpeXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
K562	MoDyRY51cXS3bX;yJIF{e2G7	M2PNWFQ5KGi{cx?=	M4PqSGdKPTBiPTCwMlMzKM7:TR?=	NXHFO5hFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1PFgzQDFpPkG4OVg5OjhzPD;hQi=>
HCT116	MlPDR5l1d3SxeHnjbZR6KGG\|c3H5	Mke2OFghcHK\|	NY\uT2tLUUN3MDC9JFAvOzJizszN	M2XRWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{SyNVQ3Lz5{MkO0NlE1PjxxYU6=
CFPAC-1	NGL4Z21EgXSxdH;4bYNqfHliYYPzZZk>	M2jJTVk3KGi{cx?=	NXfSfZZKUUN3MDC9JFAvOzVizszN	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ\|MUO1PUc,OjR4M{GzOVk9N2F-
PANC1	MULDfZRwfG:6aXPpeJkh[XO\|YYm=	NITnNmw4OiCqcoO=	MU\JR\|UxKD1iMD60JO69VQ>?	NUDkPHhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OVYzODJpPkK5OlU3OjB{PD;hQi=>
HCT116	MnLwR5l1d3SxeHnjbZR6KGG\|c3H5	M1XobVczKGi{cx?=	NEf3ZXpKSzVyIE2gNE41OSEQvF2=	Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{MkG3PVAoRjJ6MkKxO\|kxRC:jPh?=
CFPAC-1	NV;5bI5GS3m2b4TvfIlkcXS7IHHzd4F6	NEHXWFM4OiCqcoO=	NFiwbnFKSzVyIE2gNE41PyEQvF2=	MlnXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{GzOVkoRjJ2NkOxN\|U6RC:jPh?=
ACHN	NF3veYhEgXSxdH;4bYNqfHliYYPzZZk>	NI\QdWk1QCCqcoO=	MUjJR\|UxKD1iMD60PEDPxE1?	M2\tcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{SyNVQ3Lz5{MkO0NlE1PjxxYU6=
HCT116-E6	NIHWcYlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	MYC3NkBpenN?	M3XSVWlEPTBiPTCwMlUh\|ryP	MlX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
C6	MonGR5l1d3SxeHnjbZR6KGG\|c3H5	M1qySFMh\GG7cx?=	NFv5T5RKSzVyIE2gNE42ODRizszN	M3jHdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
LNCAP	Moe0R5l1d3SxeHnjbZR6KGG\|c3H5	NV;mNGxqOyCmYYnz	M1\JXmlEPTBiPTCwMlUyOiEQvF2=	MmDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
Calu1	NHHtUXZEgXSxdH;4bYNqfHliYYPzZZk>	NGL4[Ig1QCCqcoO=	NWqybXQ6UUN3MDC9JFAvPTJizszN	M1LpclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{SyNVQ3Lz5{MkO0NlE1PjxxYU6=
HT-29	MX7DfZRwfG:6aXPpeJkh[XO\|YYm=	MX[3NkBpenN?	M1nuOmlEPTBiPTCwMlUzKM7:TR?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB{NUi3OUc,OjZyMkW4O\|U9N2F-
MCF7	MmroR5l1d3SxeHnjbZR6KGG\|c3H5	MUW3NkBpenN?	MW\JR\|UxKD1iMD61O{DPxE1?	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB5NUW5Nkc,OjhyN{W1PVI9N2F-
K562	MojiRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	NIG0e484OiCqcoO=	MlOyTWM2OCB;IECuOkDPxE1?	NWLUWIxIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4O\|M4PDBpPkKwPFc{PzRyPD;hQi=>
MIAPaCa2	MoXiR5l1d3SxeHnjbZR6KGG\|c3H5	NXTKd3VROjRiaILz	NXv0S5VrUUN3MDC9JFAvPiEQvF2=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyM{K5Okc,OjV5MEOyPVY9N2F-
MDA-MB-231	NEfDW\|FEgXSxdH;4bYNqfHliYYPzZZk>	M3joeVczKGi{cx?=	MYXJR\|UxKD1iMD62OUDPxE1?	NIS0SYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCyOVg4PSd-Mk[wNlU5PzV:L3G+
BxPC3	MUTDfZRwfG:6aXPpeJkh[XO\|YYm=	MYW3NkBpenN?	NGHQR5JKSzVyIE2gNE43PyEQvF2=	M17Gd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEexPVk5Lz5{NES3NVk6QDxxYU6=
K562	NGHrTIlEgXSxdH;4bYNqfHliYYPzZZk>	MVKzJIRigXN?	NWLyW\|dQUUN3MDC9JFAvPzF6IN88US=>	NULlR5VTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO\|EyODV2PD;hQi=>
K562	M4q1c2FvfGmycn;sbYZmemG2aY\lJIF{e2G7	MnPmO\|IhcHK\|	NULpb\|RJUUN3MDC9JFAvPzR3OTFOwG0>	Mk\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2Nkm4NFkoRjF6NE[5PFA6RC:jPh?=
Bel7402	MkTQR5l1d3SxeHnjbZR6KGG\|c3H5	NGrsb2Y4OiCqcoO=	MV;JR\|UxKD1iMD64OEDPxE1?	NILGN449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
HCT116/E6	Moj6RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	NX2wTZFGPzJiaILz	MVjJR\|UxKD1iMD64PVY2KM7:TR?=	NEXheVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES2PVgxQSd-MUi0Olk5ODl:L3G+
HeLa	M2nWTWN6fG:2b4jpZ4l1gSCjc4PhfS=>	M1:1VVk3KGi{cx?=	NHXrZ49KSzVyIE2gNE46KM7:TR?=	NYK1RYVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N\|E{PTlpPkK0OlMyOzV7PD;hQi=>
CEM	MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MkfwTWM2OCB;IEGg{txO	NXfKN5l4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
HB-1	NXz3V\|lkSW62aY\pdoFtKGG\|c3H5	M2C3U\|I1KGi{cx?=	NUX4d5BkTUN3MDC9JFEh\|ryP	NYLxW5oyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1PFA2PTRpPkKwOVgxPTV2PD;hQi=>
MIAPaCa2	MXLDfZRwfG:6aXPpeJkh[XO\|YYm=	M2G2VVczKGi{cx?=	NXzhOJBCUUN3MDC9JFEvODRizszN	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5MUm5PEc,OjR2N{G5PVg9N2F-
SK-N-AS	NE[3coNEgXSxdH;4bYNqfHliYYPzZZk>	Mmm2N{Bl[Xm\|	Mly4TWM2OCB;IEGuNUDPxE1?	NHnOTHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
SW1990	NFeyb4xEgXSxdH;4bYNqfHliYYPzZZk>	MofZO\|IhcHK\|	NIrESmdKSzVyIE2gNU4zKM7:TR?=	M13iVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
A549	M{n6[GN6fG:2b4jpZ4l1gSCjc4PhfS=>	MYW3NkBpenN?	MYHJR\|UxKD1iMT60JO69VQ>?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl\|NkK0O\|Q9N2F-
HepG2	M1fOSmZ2dmO2aX;uJIF{e2G7		MWnLcUA:KDFwNDFOwG0>	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzFyMU[3OEc,OTdzMEG2O\|Q9N2F-
BL21(DE3)	MnTzSpVv[3Srb36gZZN{[Xl?		MYXLcUA:KDFwNEOg{txO	NXGyeWh1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN\|AyOzFpPkKzNlMxOTNzPD;hQi=>
U87MG	M{G4UGN6fG:2b4jpZ4l1gSCjc4PhfS=>	NIW4eVI{KGSjeYO=	M{HTTGlEPTBiPTCxMlQ6KM7:TR?=	M2XyWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
BL21(DE3)	NIi1W4xHfW6ldHnvckBie3OjeR?=		MVPLcUA:KDFwNUKg{txO	NUDVXmxFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN\|AyOzFpPkKzNlMxOTNzPD;hQi=>
HT-29	NUTNN4pwS3m2b4TvfIlkcXS7IHHzd4F6	MlvjN{Bl[Xm\|	Mmr2TWM2OCB;IEGuOVMh\|ryP	MnzFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
SW1990	NWPXfVF3S3m2b4TvfIlkcXS7IHHzd4F6		MkXwTWM2OCB;IEGuOkDPxE1?	NIHkOHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG5OVQ3Pid-MkSxPVU1PjZ:L3G+
Capan2	MmrlR5l1d3SxeHnjbZR6KGG\|c3H5	MoLUO\|IhcHK\|	M4T2R2lEPTBiPTCxMlch\|ryP	M1X2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
SW480	NWLTSJlLSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	M3vHdFczKGi{cx?=	Ml70TWM2OCB;IEGuO{DPxE1?	NXf4SoI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4O\|M4PDBpPkKwPFc{PzRyPD;hQi=>
PANC1	NVTwcWJ6S3m2b4TvfIlkcXS7IHHzd4F6	NIixOFY4OiCqcoO=	M4W3NmlEPTBiPTCxMlch\|ryP	NHnlcZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[1OlIxOid-Mkm2OVYzODJ:L3G+
HCT8	MULDfZRwfG:6aXPpeJkh[XO\|YYm=	MV:3NkBpenN?	NGnod49KSzVyIE2gNU44PCEQvF2=	M4LqT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
BL21(DE3)	NGrmbVlHfW6ldHnvckBie3OjeR?=		MkHlT40hRSBzLke1JO69VQ>?	NUn5TJpLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN\|AyOzFpPkKzNlMxOTNzPD;hQi=>
HT-29	MnzaR5l1d3SxeHnjbZR6KGG\|c3H5	NYnDW4VDPDhiaILz	M3;XXWlEPTBiPTCxMlk2KM7:TR?=	NUH2RlVWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5Olg5OjRpPkKzPVY5QDJ2PD;hQi=>
MIAPaCa2	M3jQWWN6fG:2b4jpZ4l1gSCjc4PhfS=>	M3HDSlUh\GG7cx?=	NYLXZlRFTUN3MDC9JFIh\|ryP	MlHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Nke1PVAoRjJ2OE[3OVkxRC:jPh?=
BL21(DE3)	NUT5eFdQTnWwY4Tpc44h[XO\|YYm=	NGLUUZcyKG2rbh?=	MYHLcUA:KDJwMUWg{txO	NIntTIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN\|AyOzF:L3G+
SW1990	M{TOWmN6fG:2b4jpZ4l1gSCjc4PhfS=>		NGrnXmRKSzVyIE2gNk4zKM7:TR?=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyNUeyNkc,OjVzMEW3NlI9N2F-
SW1990	MmezR5l1d3SxeHnjbZR6KGG\|c3H5	M1LESlQ5KGi{cx?=	NHLqT4dKSzVyIE2gNk4{KM7:TR?=	MkLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd7Nk[5OVAoRjJ5OU[2PVUxRC:jPh?=
NCI-H146	MWHDfZRwfG:6aXPpeJkh[XO\|YYm=	M{DkNlMh\GG7cx?=	MUnJR\|UxKD1iMj63PEDPxE1?	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
BxPC3	M{HoSmN6fG:2b4jpZ4l1gSCjc4PhfS=>	MmfkO\|IhcHK\|	NW\Ic2hEUUN3MDC9JFIvQSEQvF2=	NF36bJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
p53 deficient COLO205	Mlz2RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	NIr0eVU4OiCqcoO=	NWnkOHk3UUN3MDC9JFMh\|ryP	Mlf3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6N{O3OFAoRjJyOEezO\|QxRC:jPh?=
MiaPaCa	Mn7UR5l1d3SxeHnjbZR6KGG\|c3H5	NWLPOIY6PiCmYYnz	M1O2emlEPTBiPTCzJO69VQ>?	M4ftN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{[wNVA1Lz5{M{O2NFExPDxxYU6=
COLO205	M3zsSmN6fG:2b4jpZ4l1gSCjc4PhfS=>	NVz0OYJnPDhiaILz	NELpOJdKSzVyIE2gN{4zPCEQvF2=	NVnBVlZ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5Olg5OjRpPkKzPVY5QDJ2PD;hQi=>
MCF7	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=	NGLGb3A4OiCqcoO=	Ml;MTWM2OCB;IEOuNlgh\|ryP	NWXrOXZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N\|YzPDd2PD;hQi=>
MIAPaCa2	NES0NJNEgXSxdH;4bYNqfHliYYPzZZk>	MXW3NkBpenN?	M1XudWlEPTBiPTCzMlMh\|ryP	NYrseYVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzNlg3PTZpPkK5N\|I5PjV4PD;hQi=>
HeLa	Mny0R5l1d3SxeHnjbZR6KGG\|c3H5	NHrPZVQzPCCqcoO=	MmHCTWM2OCB;IEOuN{DPxE1?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyM{K5Okc,OjV5MEOyPVY9N2F-
FTC-133	MlP5R5l1d3SxeHnjbZR6KGG\|c3H5	MnLoNlQhcHK\|	M2HvVmlEPTBiPTCzMlM3KM7:TR?=	NWTlNYNZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N\|Y6QTRpPkK0OFM3QTl2PD;hQi=>
BxPC3	M3O1OGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MnL2OFghcHK\|	NXnnWIViT0l3MDC9JFMvPjRizszN	M4P1UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
COLO320DM	NHyx[mhEgXSxdH;4bYNqfHliYYPzZZk>	MlnxOFghcHK\|	MlrXTWM2OCB;IEOuPVIh\|ryP	NV:xc5RLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5Olg5OjRpPkKzPVY5QDJ2PD;hQi=>
HeLa	MkfaR5l1d3SxeHnjbZR6KGG\|c3H5	NEPmZ4I{KGSjeYO=	NFnPRYRKSzVyIE2gOE4yOiEQvF2=	NXLFUHFSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO\|EyODV2PD;hQi=>
8305C	Ml:5R5l1d3SxeHnjbZR6KGG\|c3H5	MXiyOEBpenN?	MW\JR\|UxKD1iND61N{DPxE1?	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR\|Nkm5OEc,OjR2M{[5PVQ9N2F-
PANC1	NYDqeFgyS3m2b4TvfIlkcXS7IHHzd4F6	NUH5cZBuPzJiaILz	NHvuNFFKSzVyIE2gOU43KM7:TR?=	NIXOVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
PANC1	Mo\oS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	NWL4e41NPzJiaILz	NFnpSpVIUTVyIE2gOU45KM7:TR?=	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7NUC4NUc,Ojh2OUWwPFE9N2F-
SK-MEL-2	NWX6fpJmS3m2b4TvfIlkcXS7IHHzd4F6	MYKzJIRigXN?	MUDJR\|UxKD1iNz6xNUDPxE1?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
CEM	NVjmflU2S3m2b4TvfIlkcXS7IHHzd4F6	NVPEOIxnOyCmYYnz	NGTwUWVKSzVyIE2gO{43KM7:TR?=	NHfKNIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0NVM2Pid-MkSzOFE{PTZ:L3G+
SW1573	NIjDe\|ZEgXSxdH;4bYNqfHliYYPzZZk>	MUm3NkBpenN?	NIDJdIVKSzVyIE2gPE4{KM7:TR?=	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzOU[wOEc,OTd2MUm2NFQ9N2F-
BxPC3	MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	MYq0PEBpenN?	MXzMR\|UxKD1iOD63NUDPxE1?	NGH5WnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+
MCF7	MX;DfZRwfG:6aXPpeJkh[XO\|YYm=	MoPuO\|IhcHK\|	NYDzfmhoUUN3MDC9JFgvQSEQvF2=	NF;4XZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC3OVU6Oid-MkiwO\|U2QTJ:L3G+
BJ	NFrkOYxEgXSxdH;4bYNqfHliYYPzZZk>	Mn\xN{Bl[Xm\|	NV7IelJtUUN3MDC9JFkvQDhizszN	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
HeLa	NW\pfoFKS3m2b4TvfIlkcXS7IHHzd4F6	M2PSRlczKGi{cx?=	MnXPTWM2OCB;IEGwJO69VQ>?	NFvrbHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zNVM2QSd-MkS2N\|E{PTl:L3G+
B16-F10-Luc-G5	M3fjV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7	MoPrNlQhcHK\|	NY\VR4FHUUN3MDC9JFEyKM7:TR?=	Mn;OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd\|NEmzN\|IoRjJ5M{S5N\|MzRC:jPh?=
HCT15	NUHHSJRWS3m2b4TvfIlkcXS7IHHzd4F6		M{naXWlEPTBiPTCxNU45KM7:TR?=	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDF6Nk[wOEc,OThzOE[2NFQ9N2F-
A549	MUHDfZRwfG:6aXPpeJkh[XO\|YYm=	NIXC[I84OiCqcoO=	NVnn[HhjUUN3MDC9JFE{NjFizszN	M2W4W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
HCT116	M1LhXmN6fG:2b4jpZ4l1gSCjc4PhfS=>		M3rrUGlEPTBiPTCxOE4{KM7:TR?=	M2\sb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MUi2OlA1Lz5zOEG4OlYxPDxxYU6=
MiaPaCa	M3z6VmN6fG:2b4jpZ4l1gSCjc4PhfS=>	M3:zeFczKGi{cx?=	M3PPXGlEPTBiPTCxO{4yKM7:TR?=	M4PC[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEi5OlI3Lz5{M{S4PVYzPjxxYU6=
AG6000	M2KyUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NWDOSld[UUN3MDC9JFIxKM7:TR?=	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
AG6000	NYnKeXBWT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NVLCToF1UUN3MDC9JFIxKM7:TR?=	NIrpZ4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wNlQ3PCd-MUe2NFI1PjR:L3G+
BxPC3	MYjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?	MkizO\|IhcHK\|	M4TncWlEPTBiPTCyNE4zKM7:TR?=	NGfwOFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O\|Y3OzN:L3G+
CV1	NIX4bFBEgXSxdH;4bYNqfHliYYPzZZk>	NGfzR\|k4OiCqcoO=	NHr3cGFKSzVyIE2gNlEvQSEQvF2=	NHKzTnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S4PVYzPid-MkO0PFk3OjZ:L3G+
AsPC1	NGjzUJVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	NV60UplvPzJiaILz	NEjo[Y9KSzVyIE2gNlYvQCEQvF2=	NICwe5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O\|Y3OzN:L3G+
PANC1	M2PrXGFvfGmycn;sbYZmemG2aY\lJIF{e2G7	Ml6yO\|IhcHK\|	NH\reoZKSzVyIE2gN\|AvPCEQvF2=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV5Nk[zN{c,Ojh3N{[2N\|M9N2F-
MIAPaCa2	NHLBSpdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NXrtU4M6PDhiaILz	NULEUldZT0l3MDC9JFM2Njh\|IN88US=>	NWn1SZFSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NVI6ODhpPkKyOVEzQTB6PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p70S6K1 / p-S6 / HIF-1α; PubMed: 27765914 Oncotarget Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control. LC3-I / LC3-II; PubMed: 27384485 Oncotarget A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h). pS-ERα / ERα / pERK / ERK; PubMed: 27384485 Oncotarget MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected. caspase-3 / caspase-8 / caspase-9; PubMed: 16307741 FEBS Lett H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control. p-JNK / JNK / p-ATF2 / p-c-Jun; PubMed: 16307741 FEBS Lett Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h.	27765914 27384485 16307741
Immunofluorescence	BMI1; PubMed: 27177084 Oncotarget Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining.	27177084
Growth inhibition assay	Cell viability ; PubMed: 27765914 Oncotarget A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.	27765914

In vivo

Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. ^[2]

Protocol

Cell Research:^[2]

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Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
Concentrations: 0.2 μM
Incubation Time: 24 hours or 48 hours
Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
(Only for Reference)

Animal Research:^[2]

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Animal Models: Athymic nude mice with MIA PaCa-2 cells
Dosages: 50 mg/kg or 100 mg/kg
Administration: Administered via i.p.
(Only for Reference)

References

Solubility (25°C)

In vitro	Water	19 mg/mL (63.4 mM)
	DMSO	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): Saline For best results, use promptly after mixing.	19mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Gemcitabine HCl SDF

Molecular Weight	299.66
Formula	C₉H₁₁F₂N₃O₄.HCI
CAS No.	122111-03-9
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	LY188011
Smiles	C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04634539	Not yet recruiting	Drug: Gemcitabine\|Drug: Nab-paclitaxel\|Drug: L-glutamine	Advanced Pancreatic Adenocarcinoma\|Pancreatic Cancer\|Pancreatic Ductal Adenocarcinoma	Jun Gong MD\|Emmaus Medical Inc.\|Cedars-Sinai Medical Center	January 1 2021	Phase 1
NCT04383119	Not yet recruiting	Drug: Trabectedin\|Drug: Gemcitabine	Leiomyosarcoma of Ovary\|Soft Tissue Sarcoma	Italian Sarcoma Group\|PharmaMar	January 15 2021	Phase 2
NCT04616534	Not yet recruiting	Drug: Elimusertib\|Drug: Gemcitabine	Advanced Fallopian Tube Carcinoma\|Advanced Malignant Solid Neoplasm\|Advanced Ovarian Carcinoma\|Advanced Pancreatic Adenocarcinoma\|Advanced Primary Peritoneal Carcinoma\|High Grade Ovarian Serous Adenocarcinoma\|Platinum-Resistant Fallopian Tube Carcinoma\|Platinum-Resistant Ovarian Carcinoma\|Platinum-Resistant Primary Peritoneal Carcinoma\|Stage II Pancreatic Cancer AJCC v8\|Stage IIA Pancreatic Cancer AJCC v8\|Stage IIB Pancreatic Cancer AJCC v8\|Stage III Fallopian Tube Cancer AJCC v8\|Stage III Ovarian Cancer AJCC v8\|Stage III Pancreatic Cancer AJCC v8\|Stage III Primary Peritoneal Cancer AJCC v8\|Stage IIIA Fallopian Tube Cancer AJCC v8\|Stage IIIA Ovarian Cancer AJCC v8\|Stage IIIA Primary Peritoneal Cancer AJCC v8\|Stage IIIA1 Fallopian Tube Cancer AJCC v8\|Stage IIIA1 Ovarian Cancer AJCC v8\|Stage IIIA2 Fallopian Tube Cancer AJCC v8\|Stage IIIA2 Ovarian Cancer AJCC v8\|Stage IIIB Fallopian Tube Cancer AJCC v8\|Stage IIIB Ovarian Cancer AJCC v8\|Stage IIIB Primary Peritoneal Cancer AJCC v8\|Stage IIIC Fallopian Tube Cancer AJCC v8\|Stage IIIC Ovarian Cancer AJCC v8\|Stage IIIC Primary Peritoneal Cancer AJCC v8\|Stage IV Fallopian Tube Cancer AJCC v8\|Stage IV Ovarian Cancer AJCC v8\|Stage IV Pancreatic Cancer AJCC v8\|Stage IV Primary Peritoneal Cancer AJCC v8\|Stage IVA Fallopian Tube Cancer AJCC v8\|Stage IVA Ovarian Cancer AJCC v8\|Stage IVA Primary Peritoneal Cancer AJCC v8\|Stage IVB Fallopian Tube Cancer AJCC v8\|Stage IVB Ovarian Cancer AJCC v8\|Stage IVB Primary Peritoneal Cancer AJCC v8	National Cancer Institute (NCI)	December 31 2020	Phase 1
NCT04657068	Not yet recruiting	Drug: ART0380\|Drug: Gemcitabine	Advanced Cancer\|Metastatic Cancer\|Ovarian Cancer	Artios Pharma Ltd	December 2020	Phase 1\|Phase 2
NCT04640623	Not yet recruiting	Drug: TAR-200\|Drug: Cetrelimab	Urinary Bladder Neoplasms	Janssen Research & Development LLC	December 17 2020	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
What’s the difference between S1714 and S1149 and which one is better?
Answer:
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

DNA/RNA Synthesis Signaling Pathway Map

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Gemcitabine HCl