Gemcitabine (LY-188011) HCl

Catalog No.S1149 Synonyms: NSC 613327

For research use only.

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Gemcitabine (LY-188011) HCl Chemical Structure

CAS No. 122111-03-9

Selleck's Gemcitabine (LY-188011) HCl has been cited by 136 publications

Purity & Quality Control

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Biological Activity

Description Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
Features Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.
Targets
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPjWJVKSzVyPUKuPUDDuSBzLkigcm0> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM/dCK−/− NI\FdG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HhPWlEPTB;MkSwMlQhyrFiMkmuNEDPxE1? M2XwZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 wt MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTFwMzFCtUAxNjNibl2= NXTJXHg1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 10K NV3LT2loT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWL1dWZMUUN3ME2yNk4zKMLzIEOuO{DPxE1? NILXXYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
TC-1  M2HMZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nrPWlEPTB;MUSuO{DDuSB{Lkigcm0> NVrDbmlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
TC-1-GR M{X0[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITFWFVKSzVyPUO2MlchyrFiNT6xJO69VQ>? M1ezOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
MIA PaCa-2 NHnxS5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTR7LkegxtEhOTdwNzDuUS=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
PANC-1 NIi2b5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXKSGJHUUN3ME2+JFQxOCEQvF2= NW\4eodzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPZTWM2OD1{LkmgxtEhOS56IH7N NWLBc2ZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM-AraC-8C NXzXcmRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELFSWFKSzVyPUm5PE45KMLzIEmuOEBvVQ>? NVe2cYt2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM  NHn5UpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLpXm1CPzJiaB?= M4PZPGlEPTB;Mj6wJOKyKDBwNjDuUS=> NELNToQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVg1Oyd-MkG4OVE5PDN:L3G+
MV-4-11 NVHmNopKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGjCcIpKSzVyIE2gNE4xODB3IH7N MnTZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ES4 NETYfYpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\4TWM2OCB;IECuNFAxPyCwTR?= NIXoNXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ACHN MoPHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmWwTWM2OCB;IECuNFAxQSCwTR?= NEnjd2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KYSE-510 MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXXFfmJ6UUN3MDC9JFAvODBzIH7N NETISZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
EW-7 NUjJUWxrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYfJR|UxKD1iMD6wNFI3KG6P NWHOWlFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
BFTC-905 MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWnJR|UxKD1iMD6wNFUyKG6P NVj2UXJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KE-37 NXH0cHpUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGrFcY9KSzVyIE2gNE4xODV4IH7N Ml;uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SBC-5 M1TuTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MULJR|UxKD1iMD6wNFU4KG6P MkXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
NKM-1 MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWDIOWk2UUN3MDC9JFAvODB5MTDuUS=> MnXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RH-1 MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXLJR|UxKD1iMD6wNFczKG6P MkX3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ALL-PO NYOxSWxFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVnJR|UxKD1iMD6wNFg{KG6P NWHqOG1sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
QIMR-WIL MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW\hcJlxUUN3MDC9JFAvODB6OTDuUS=> M3vL[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
A375 NHfq[GJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3z4OGlEPTBiPTCwMlAxQTlibl2= NF7sZoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SIG-M5 Mm[2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEnHdoxKSzVyIE2gNE4xOTB2IH7N M2jRPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
KGN MlLaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGjpZmlKSzVyIE2gNE4xOTB6IH7N MkX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
EW-13 MmDZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIHWR3lKSzVyIE2gNE4xOTF{IH7N NF;DPIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MljrTWM2OCB;IECuNFE3KG6P MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
PSN1 NX\ZVVBjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVrJR|UxKD1iMD6wNVY2KG6P M1XuR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HUTU-80 MoXVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGPYSnNKSzVyIE2gNE4xOTZ4IH7N MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
786-0 M3LMcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlTkTWM2OCB;IECuNFI{KG6P NVLhcFNZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-16 NXXBSXR{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3zMOGlEPTBiPTCwMlAzOyCwTR?= NWLtTXlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ES1 M4Lyfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX\CRpAxUUN3MDC9JFAvODJ4ODDuUS=> NUXZd5hzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
RKO NXfIcpJET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml;YTWM2OCB;IECuNFI4QCCwTR?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ESS-1 NUnxN|hNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJR|UxKD1iMD6wNlg3KG6P NUHkSlRPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
SK-UT-1 M1L1bmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV\aSmI{UUN3MDC9JFAvODJ7NzDuUS=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
LB2241-RCC NF2ydFlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4jjdGlEPTBiPTCwMlA{OThibl2= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
CHL-1 NWThXIk4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn7vTWM2OCB;IECuNFMzPCCwTR?= NF3jXJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SW1783 Mk\BS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M17PXWlEPTBiPTCwMlA{OzZibl2= Ml3jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
MEL-JUSO M3TPPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV7JR|UxKD1iMD6wN|kyKG6P NIDnOZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HT-29 M{\XOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEHyenhKSzVyIE2gNE4xPDF|IH7N NUDtfYd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
SNG-M MmjOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFHYTpJKSzVyIE2gNE4xPDJ3IH7N NEnYXY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
TE-15 NF3ZbXRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJR|UxKD1iMD6wOFY1KG6P NFnxWHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HOS M2HpRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYPJR|UxKD1iMD6wOFghdk1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
BB65-RCC M4HlbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnjNTWM2OCB;IECuNFUyOiCwTR?= NH;jdIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HCE-4 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXTVToNqUUN3MDC9JFAvODV{ODDuUS=> MnLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
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SK-N-AS NHT5PFNEgXSxdH;4bYNqfHliYYPzZZk> M2HPbFMh\GG7cx?= M{XjPGlEPTBiPTCxMlEh|ryP Mn;4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
SW1990 M1zkSGN6fG:2b4jpZ4l1gSCjc4PhfS=> NXTkeFdIPzJiaILz MXvJR|UxKD1iMT6yJO69VQ>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl|NkK0O|Q9N2F-
A549 MVrDfZRwfG:6aXPpeJkh[XO|YYm= NFL6S|k4OiCqcoO= NWq3V|dGUUN3MDC9JFEvPCEQvF2= NX;XNZJNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
HepG2 MlLVSpVv[3Srb36gZZN{[Xl? NUniS25kU21iPTCxMlQh|ryP NH;2e|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GwNVY4PCd-MUexNFE3PzR:L3G+
BL21(DE3) NHXlS2xHfW6ldHnvckBie3OjeR?= NGmyU49MdSB;IEGuOFMh|ryP NWO0TYdVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|AyOzFpPkKzNlMxOTNzPD;hQi=>
U87MG Mo\2R5l1d3SxeHnjbZR6KGG|c3H5 MmDHN{Bl[Xm| MY\JR|UxKD1iMT60PUDPxE1? NYrUT49wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
BL21(DE3) NF;NZ5pHfW6ldHnvckBie3OjeR?= MXPLcUA:KDFwNUKg{txO NGLGRZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN|AyOzF:L3G+
HT-29 M2[0UGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mn7aN{Bl[Xm| Mo\STWM2OCB;IEGuOVMh|ryP NXPjSWxmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
SW1990 NXTqS5c1S3m2b4TvfIlkcXS7IHHzd4F6 NF2zRVVKSzVyIE2gNU43KM7:TR?= M{XVbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUm1OFY3Lz5{NEG5OVQ3PjxxYU6=
Capan2 NEHVUJlEgXSxdH;4bYNqfHliYYPzZZk> MnfNO|IhcHK| NYXnUJViUUN3MDC9JFEvPyEQvF2= NXrvSVBCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
SW480 Mny4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NHvze2w4OiCqcoO= NH7VfZNKSzVyIE2gNU44KM7:TR?= NWX4N3NmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4O|M4PDBpPkKwPFc{PzRyPD;hQi=>
PANC1 M1f0V2N6fG:2b4jpZ4l1gSCjc4PhfS=> NETRVo44OiCqcoO= NETPcXFKSzVyIE2gNU44KM7:TR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3NkKwNkc,Ojl4NU[yNFI9N2F-
HCT8 MUXDfZRwfG:6aXPpeJkh[XO|YYm= Mmq1O|IhcHK| NH23[ldKSzVyIE2gNU44PCEQvF2= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl|NkK0O|Q9N2F-
BL21(DE3) M{T6bmZ2dmO2aX;uJIF{e2G7 MmWyT40hRSBzLke1JO69VQ>? MnWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
HT-29 MnLaR5l1d3SxeHnjbZR6KGG|c3H5 M2jUR|Q5KGi{cx?= M2r3U2lEPTBiPTCxMlk2KM7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OEiyOEc,OjN7Nki4NlQ9N2F-
MIAPaCa2 NFz0eJBEgXSxdH;4bYNqfHliYYPzZZk> M3ntR|Uh\GG7cx?= NWT2dXhITUN3MDC9JFIh|ryP NEHmXJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi2O|U6OCd-MkS4Olc2QTB:L3G+
BL21(DE3) Mki4SpVv[3Srb36gZZN{[Xl? NUP5XpNMOSCvaX6= MnTTT40hRSB{LkG1JO69VQ>? MmqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
SW1990 NIr0W4dEgXSxdH;4bYNqfHliYYPzZZk> NFLxVYVKSzVyIE2gNk4zKM7:TR?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyNUeyNkc,OjVzMEW3NlI9N2F-
SW1990 M3r6e2N6fG:2b4jpZ4l1gSCjc4PhfS=> M{HkO|Q5KGi{cx?= Mo\nTWM2OCB;IEKuN{DPxE1? NYXONYNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke5OlY6PTBpPkK3PVY3QTVyPD;hQi=>
NCI-H146 M4D3dGN6fG:2b4jpZ4l1gSCjc4PhfS=> NHvxdI0{KGSjeYO= NGnzVmJKSzVyIE2gNk44QCEQvF2= NH3IcYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
BxPC3 MYrDfZRwfG:6aXPpeJkh[XO|YYm= MWG3NkBpenN? NXSwNZZLUUN3MDC9JFIvQSEQvF2= NIfHNmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
p53 deficient COLO205 M1nNbGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MV63NkBpenN? MWjJR|UxKD1iMzFOwG0> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5M{e0NEc,OjB6N{O3OFA9N2F-
MiaPaCa MYnDfZRwfG:6aXPpeJkh[XO|YYm= NFm4NVk3KGSjeYO= M3nHd2lEPTBiPTCzJO69VQ>? NEfXbZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O2NFExPCd-MkOzOlAyODR:L3G+
COLO205 MkXVR5l1d3SxeHnjbZR6KGG|c3H5 MWq0PEBpenN? NFO3UmdKSzVyIE2gN{4zPCEQvF2= MmnMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ|OU[4PFI1RC:jPh?=
MCF7 M2D2fGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mm\6O|IhcHK| M4X0NGlEPTBiPTCzMlI5KM7:TR?= NEXRbZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
MIAPaCa2 MkjwR5l1d3SxeHnjbZR6KGG|c3H5 MlvHO|IhcHK| NITNOnhKSzVyIE2gN{4{KM7:TR?= M{GzeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{K4OlU3Lz5{OUOyPFY2PjxxYU6=
HeLa MWTDfZRwfG:6aXPpeJkh[XO|YYm= Ml;KNlQhcHK| M3qzUmlEPTBiPTCzMlMh|ryP NHHjblc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUewN|I6Pid-MkW3NFMzQTZ:L3G+
FTC-133 M{O3eGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVTVd5BYOjRiaILz NInoOHdKSzVyIE2gN{4{PiEQvF2= MmHsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3 M3XDTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXi0UoNEPDhiaILz NFT0UJNIUTVyIE2gN{43PCEQvF2= M{PGWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
COLO320DM MnPkR5l1d3SxeHnjbZR6KGG|c3H5 MmHqOFghcHK| M1rSNWlEPTBiPTCzMlkzKM7:TR?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OEiyOEc,OjN7Nki4NlQ9N2F-
HeLa NHPGSW9EgXSxdH;4bYNqfHliYYPzZZk> MkX3N{Bl[Xm| NFPzWHBKSzVyIE2gOE4yOiEQvF2= NIe0TFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
8305C MoraR5l1d3SxeHnjbZR6KGG|c3H5 NH;TRVQzPCCqcoO= NVuySpVRUUN3MDC9JFQvPTNizszN M2LFeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEO2PVk1Lz5{NESzOlk6PDxxYU6=
PANC1 M{nGWWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3raXFczKGi{cx?= NGCxN2hKSzVyIE2gOU43KM7:TR?= NGrPdm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
PANC1 NIDjbXRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoPnO|IhcHK| MXrHTVUxKD1iNT64JO69VQ>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7NUC4NUc,Ojh2OUWwPFE9N2F-
SK-MEL-2 MmLuR5l1d3SxeHnjbZR6KGG|c3H5 M2SxNlMh\GG7cx?= NVXUd2tPUUN3MDC9JFcvOTFizszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
CEM MnHSR5l1d3SxeHnjbZR6KGG|c3H5 NW\2U4cyOyCmYYnz MlG0TWM2OCB;IEeuOkDPxE1? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN2MUO1Okc,OjR|NEGzOVY9N2F-
SW1573 NHX3dnJEgXSxdH;4bYNqfHliYYPzZZk> NIjXeHQ4OiCqcoO= M1iyOGlEPTBiPTC4MlMh|ryP NUnSepZiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVk3ODRpPkG3OFE6PjB2PD;hQi=>
BxPC3 M17seGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYq0PEBpenN? NWTPOnJMVEN3MDC9JFgvPzFizszN NWrte2tDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NVI6ODhpPkKyOVEzQTB6PD;hQi=>
MCF7 NXz5SZpnS3m2b4TvfIlkcXS7IHHzd4F6 NI\sXFE4OiCqcoO= NITpfYVKSzVyIE2gPE46KM7:TR?= NHXXUoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC3OVU6Oid-MkiwO|U2QTJ:L3G+
BJ Mnn0R5l1d3SxeHnjbZR6KGG|c3H5 M4jrNlMh\GG7cx?= MULJR|UxKD1iOT64PEDPxE1? NH7mW|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
HeLa Mlf4R5l1d3SxeHnjbZR6KGG|c3H5 NYPSPJNoPzJiaILz NXjLVYVqUUN3MDC9JFExKM7:TR?= MknSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{GzOVkoRjJ2NkOxN|U6RC:jPh?=
B16-F10-Luc-G5 Mo\lRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mmr2NlQhcHK| MoDDTWM2OCB;IEGxJO69VQ>? MmTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd|NEmzN|IoRjJ5M{S5N|MzRC:jPh?=
HCT15 M362d2N6fG:2b4jpZ4l1gSCjc4PhfS=> MmT0TWM2OCB;IEGxMlgh|ryP NHPEbHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG4OlYxPCd-MUixPFY3ODR:L3G+
A549 NVrYSWV[S3m2b4TvfIlkcXS7IHHzd4F6 MlXCO|IhcHK| MoLmTWM2OCB;IEGzMlEh|ryP Mnm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUm2NFQoRjF5NEG5OlA1RC:jPh?=
HCT116 NVTsOZROS3m2b4TvfIlkcXS7IHHzd4F6 NUTRVIE3UUN3MDC9JFE1NjNizszN Mmr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzOE[2NFQoRjF6MUi2OlA1RC:jPh?=
MiaPaCa NXe1[GozS3m2b4TvfIlkcXS7IHHzd4F6 NInEZWY4OiCqcoO= MlfyTWM2OCB;IEG3MlEh|ryP NYjmTFhbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFk3OjZpPkKzOFg6PjJ4PD;hQi=>
AG6000 NHS0XnlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJR|UxKD1iMkCg{txO M4X4OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
AG6000 NFLpdGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV\DdmdZUUN3MDC9JFIxKM7:TR?= M3;w[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
BxPC3 MXvBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MYC3NkBpenN? MYLJR|UxKD1iMkCuNkDPxE1? NG\YbJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
CV1 NYDWO5N4S3m2b4TvfIlkcXS7IHHzd4F6 NVqwe5RuPzJiaILz Mmi0TWM2OCB;IEKxMlkh|ryP NXrRfXJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFk3OjZpPkKzOFg6PjJ4PD;hQi=>
AsPC1 M2XYcmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NELEVXk4OiCqcoO= Mme4TWM2OCB;IEK2Mlgh|ryP NVPw[WN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O|Y3OzNpPkK4OVc3PjN|PD;hQi=>
PANC1 M1\YO2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 MlXlO|IhcHK| MorXTWM2OCB;IEOwMlQh|ryP NELtdlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
MIAPaCa2 NUfaRlFPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWfPcZg4PDhiaILz M1zzRmdKPTBiPTCzOU45OyEQvF2= M1S3V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
Assay
Methods Test Index PMID
Western blot p-JNK / JNK / p-ATF2 / p-c-Jun ; caspase-3 / caspase-8 / caspase-9 ; pS-ERα / ERα / pERK / ERK ; LC3-I / LC3-II ; p70S6K1 / p-S6 / HIF-1α 16307741 27384485 27765914
Immunofluorescence BMI1 27177084
Growth inhibition assay Cell viability 27765914
In vivo Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. [2]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
  • Concentrations: 0.2 μM
  • Incubation Time: 24 hours or 48 hours
  • Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
Animal Research:[2]
  • Animal Models: Athymic nude mice with MIA PaCa-2 cells
  • Dosages: 50 mg/kg or 100 mg/kg
  • Administration: Administered via i.p.

Solubility (25°C)

In vitro

Water 19 mg/mL
(63.4 mM)
DMSO Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.

19mg/mL

Chemical Information

Molecular Weight 299.66
Formula

C9H11F2N3O4.HCI

CAS No. 122111-03-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05181462 Not yet recruiting Drug: Carboplatin|Drug: Gemcitabine|Drug: Nadunolimab Triple Negative Breast Cancer Cantargia AB|Spanish Breast Cancer Research Group January 2022 Phase 1|Phase 2
NCT05141149 Not yet recruiting Drug: PBP1510 (400mg/16mL)|Drug: Gemcitabine (1000 mg/m^2) Pancreatic Cancer Prestige Biopharma Limited January 2022 Phase 1|Phase 2
NCT05147272 Not yet recruiting Drug: RP-6306 (oral PKMYT1 inhibitor) Adult Solid Tumor Repare Therapeutics December 2021 Phase 1
NCT05093322 Not yet recruiting Drug: Surufatinib in combination with Gemcitabine Solid Tumor|Lymphoma|Osteosarcoma|Ewing Sarcoma|Rhabdomyosarcoma|Non-rhabdomyosarcoma Soft Tissue Sarcoma Hutchison Medipharma Limited October 30 2021 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
What’s the difference between S1714 and S1149 and which one is better?

Answer:
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

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