Gemcitabine (LY-188011) HCl

For research use only.

Catalog No.S1149 Synonyms: NSC 613327

107 publications

Gemcitabine (LY-188011) HCl Chemical Structure

CAS No. 122111-03-9

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

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Selleck's Gemcitabine (LY-188011) HCl has been cited by 107 publications

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
Features Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.
Targets
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM M1HmRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4j6cmlEPTB;Mj65JOKyKDFwODDuUS=> M1WyOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM/dCK−/− MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjmNlNsUUN3ME2yOFAvPCEEsTCyPU4xKM7:TR?= NYTpfHA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 wt NX;udYE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7FdYN4UUN3ME2xMlMhyrFiMD6zJI5O NGjaOFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
L1210 10K NGfTTpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrqPYJvUUN3ME2yNk4zKMLzIEOuO{DPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
TC-1  NYX1ZWV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnSTWM2OD1zND63JOKyKDJwODDuUS=> M1LEdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
TC-1-GR M2nVWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXm3bm1iUUN3ME2zOk44KMLzIEWuNUDPxE1? NEjaSnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
MIA PaCa-2 NGjyfWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\Q[mlEPTB;NEmuO{DDuSBzNz63JI5O M2LqXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
PANC-1 M2XhVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFj3VnJKSzVyPU6gOFAxKM7:TR?= M2H0U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM NEfHUJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXu3ZpFIUUN3ME2yMlkhyrFiMT64JI5O NFXyc5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM-AraC-8C NV[yZWF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;1U3FKSzVyPUm5PE45KMLzIEmuOEBvVQ>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM  M{mw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXRb4UzPzJiaB?= NF;4[lNKSzVyPUKuNEDDuSByLk[gcm0> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUi0N{c,OjF6NUG4OFM9N2F-
MV-4-11 NIfq[2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEL0SJpKSzVyIE2gNE4xODB3IH7N NWT3RWp[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ES4 M2K1UWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUfGeWtwUUN3MDC9JFAvODByNzDuUS=> M2jXbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ACHN NHPMbGdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlToTWM2OCB;IECuNFAxQSCwTR?= M4LNPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
KYSE-510 M3vhOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXfJOoh2UUN3MDC9JFAvODBzIH7N Mom5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
EW-7 NUnUN|JqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkjWTWM2OCB;IECuNFAzPiCwTR?= NGjUVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
BFTC-905 NH\XRpFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1\KPWlEPTBiPTCwMlAxPTFibl2= MojGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
KE-37 M3jvOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX\mPZdqUUN3MDC9JFAvODB3NjDuUS=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
SBC-5 Mn3zS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmX4TWM2OCB;IECuNFA2PyCwTR?= NFj6dI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NKM-1 M1Lyd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{nSSGlEPTBiPTCwMlAxPzFibl2= M{m3V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
RH-1 M1;hN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3GwZmlEPTBiPTCwMlAxPzJibl2= MnTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ALL-PO MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoTITWM2OCB;IECuNFA5OyCwTR?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
QIMR-WIL NHvQdGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHzNNYtKSzVyIE2gNE4xODh7IH7N NHzWdmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375 NWSzXWdyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkHqTWM2OCB;IECuNFA6QSCwTR?= MliyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SIG-M5 NFHtV4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmrUTWM2OCB;IECuNFExPCCwTR?= NHLCe|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KGN NVTlR2p7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlvqTWM2OCB;IECuNFExQCCwTR?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-13 MljoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnvxTWM2OCB;IECuNFEyOiCwTR?= NHj6Zmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 NEHJNFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUDheI44UUN3MDC9JFAvODF4IH7N NXvoTHdPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
PSN1 MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2X5XmlEPTBiPTCwMlAyPjVibl2= NXLJcIpIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
HUTU-80 MnvkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEHpfVlKSzVyIE2gNE4xOTZ4IH7N NVPXZYlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
786-0 MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1XJbmlEPTBiPTCwMlAzOyCwTR?= M1HOTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
EW-16 MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWTJR|UxKD1iMD6wNlMhdk1? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ES1 NFrpd4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYjJR|UxKD1iMD6wNlY5KG6P M122[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
RKO NGCxSlhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJR|UxKD1iMD6wNlc5KG6P MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
ESS-1 MkLuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4L5fmlEPTBiPTCwMlAzQDZibl2= NW\rOYdtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
SK-UT-1 MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYfJR|UxKD1iMD6wNlk4KG6P NYLpeI9xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
LB2241-RCC M2TmRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{nnPWlEPTBiPTCwMlA{OThibl2= NH72WnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
CHL-1 MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoO3TWM2OCB;IECuNFMzPCCwTR?= MoPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SW1783 NHPhN4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mlu1TWM2OCB;IECuNFM{PiCwTR?= MkjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
MEL-JUSO NFrpU2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXfJR|UxKD1iMD6wN|kyKG6P MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HT-29 M2P2V2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHz3NllKSzVyIE2gNE4xPDF|IH7N MnHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SNG-M MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3zGUGlEPTBiPTCwMlA1OjVibl2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
TE-15 MlLpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4XEW2lEPTBiPTCwMlA1PjRibl2= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HOS MmH2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1z0PWlEPTBiPTCwMlA1QCCwTR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
BB65-RCC NUnGUnlRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkjVTWM2OCB;IECuNFUyOiCwTR?= M3nZdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HCE-4 MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnviTWM2OCB;IECuNFUzQCCwTR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
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HepG2 NWTuVldHTnWwY4Tpc44h[XO|YYm= MmfET40hRSBzLkSg{txO MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzFyMU[3OEc,OTdzMEG2O|Q9N2F-
BL21(DE3) M1jONGZ2dmO2aX;uJIF{e2G7 NFf3[otMdSB;IEGuOFMh|ryP Ml3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
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HT-29 MYnDfZRwfG:6aXPpeJkh[XO|YYm= Mlj1N{Bl[Xm| MX\JR|UxKD1iMT61N{DPxE1? M2H0cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990 NWmxfWtUS3m2b4TvfIlkcXS7IHHzd4F6 NFLCXVhKSzVyIE2gNU43KM7:TR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF7NUS2Okc,OjRzOUW0OlY9N2F-
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HCT8 NGXEV4pEgXSxdH;4bYNqfHliYYPzZZk> NFrNO5c4OiCqcoO= M3H2VWlEPTBiPTCxMlc1KM7:TR?= NH33cWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
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HT-29 MUDDfZRwfG:6aXPpeJkh[XO|YYm= MlvQOFghcHK| MWnJR|UxKD1iMT65OUDPxE1? NEfnXpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MIAPaCa2 NIHSUFhEgXSxdH;4bYNqfHliYYPzZZk> M3nJNlUh\GG7cx?= MUfFR|UxKD1iMjFOwG0> NY\FT5h4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4Olc2QTBpPkK0PFY4PTlyPD;hQi=>
BL21(DE3) NEXBO3FHfW6ldHnvckBie3OjeR?= MlXKNUBucW5? NGPOT|lMdSB;IEKuNVUh|ryP MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ|MEGzNUc,OjN{M{CxN|E9N2F-
SW1990 NEDUNZpEgXSxdH;4bYNqfHliYYPzZZk> Ml7LTWM2OCB;IEKuNkDPxE1? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyNUeyNkc,OjVzMEW3NlI9N2F-
SW1990 NEPu[mdEgXSxdH;4bYNqfHliYYPzZZk> NF\zWIM1QCCqcoO= MUjJR|UxKD1iMj6zJO69VQ>? MlfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd7Nk[5OVAoRjJ5OU[2PVUxRC:jPh?=
NCI-H146 MkjDR5l1d3SxeHnjbZR6KGG|c3H5 MXqzJIRigXN? M1rPT2lEPTBiPTCyMlc5KM7:TR?= M3jsXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
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p53 deficient COLO205 M3r3ZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGLrZYY4OiCqcoO= NXzGO2RNUUN3MDC9JFMh|ryP NFXzeVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N|c1OCd-MkC4O|M4PDB:L3G+
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COLO205 MnXYR5l1d3SxeHnjbZR6KGG|c3H5 MlGyOFghcHK| M33POmlEPTBiPTCzMlI1KM7:TR?= M{PmeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[4PFI1Lz5{M{m2PFgzPDxxYU6=
MCF7 MnvxR5l1d3SxeHnjbZR6KGG|c3H5 M4jNR|czKGi{cx?= MmTSTWM2OCB;IEOuNlgh|ryP NYjRcZZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
MIAPaCa2 MXXDfZRwfG:6aXPpeJkh[XO|YYm= M3X0b|czKGi{cx?= Mn[yTWM2OCB;IEOuN{DPxE1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN{OE[1Okc,Ojl|Mki2OVY9N2F-
HeLa NIXDOW1EgXSxdH;4bYNqfHliYYPzZZk> MmnGNlQhcHK| MW\JR|UxKD1iMz6zJO69VQ>? Mo\HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEOyPVYoRjJ3N{CzNlk3RC:jPh?=
FTC-133 NVPKb2w5S3m2b4TvfIlkcXS7IHHzd4F6 M1v2fVI1KGi{cx?= NUHzdGVMUUN3MDC9JFMvOzZizszN MmrQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3 NICwdmpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4TEXFQ5KGi{cx?= M3\vO2dKPTBiPTCzMlY1KM7:TR?= NXLUcIhKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NVI6ODhpPkKyOVEzQTB6PD;hQi=>
COLO320DM MUjDfZRwfG:6aXPpeJkh[XO|YYm= M33nbFQ5KGi{cx?= MknVTWM2OCB;IEOuPVIh|ryP NHjHXHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
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8305C NF7uZ2VEgXSxdH;4bYNqfHliYYPzZZk> MmPXNlQhcHK| NETOTJpKSzVyIE2gOE42OyEQvF2= NUfUSId{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N|Y6QTRpPkK0OFM3QTl2PD;hQi=>
PANC1 MlXGR5l1d3SxeHnjbZR6KGG|c3H5 M4PNRVczKGi{cx?= NUj6VY94UUN3MDC9JFUvPiEQvF2= M1PVVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
PANC1 NY\udYo3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmizO|IhcHK| MnSwS2k2OCB;IEWuPEDPxE1? MoPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2OUWwPFEoRjJ6NEm1NFgyRC:jPh?=
SK-MEL-2 MUPDfZRwfG:6aXPpeJkh[XO|YYm= NHzFbms{KGSjeYO= NXK1emJYUUN3MDC9JFcvOTFizszN M2jXc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
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SW1573 MmH4R5l1d3SxeHnjbZR6KGG|c3H5 NIfLU3o4OiCqcoO= NFGzXHRKSzVyIE2gPE4{KM7:TR?= M{XRZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
BxPC3 NEjQWVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF7QdmM1QCCqcoO= NGjidYZNSzVyIE2gPE44OSEQvF2= M4jxflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
MCF7 NYf0VJRlS3m2b4TvfIlkcXS7IHHzd4F6 Mmr1O|IhcHK| MojJTWM2OCB;IEiuPUDPxE1? NY\WVmlFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwO|U2QTJpPkK4NFc2PTl{PD;hQi=>
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B16-F10-Luc-G5 M{W5OWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NE\hS3UzPCCqcoO= MWXJR|UxKD1iMUGg{txO NV7yb4c2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezOFk{OzJpPkK3N|Q6OzN{PD;hQi=>
HCT15 NVvVPJp7S3m2b4TvfIlkcXS7IHHzd4F6 MkLmTWM2OCB;IEGxMlgh|ryP M1zxSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MUi2OlA1Lz5zOEG4OlYxPDxxYU6=
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AG6000 MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWT6cpVmUUN3MDC9JFIxKM7:TR?= M2ThW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
AG6000 MoTIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXTkeXJmUUN3MDC9JFIxKM7:TR?= NITYd3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[wNlQ3PCd-MUe2NFI1PjR:L3G+
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CV1 MnLGR5l1d3SxeHnjbZR6KGG|c3H5 MVW3NkBpenN? NHXNfZlKSzVyIE2gNlEvQSEQvF2= NVzj[JlkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFk3OjZpPkKzOFg6PjJ4PD;hQi=>
AsPC1 M1;NfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NIrxcIE4OiCqcoO= NY\Ge5hyUUN3MDC9JFI3NjhizszN NXfLUpFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O|Y3OzNpPkK4OVc3PjN|PD;hQi=>
PANC1 NXHLZm1DSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NIOxcFY4OiCqcoO= MYTJR|UxKD1iM{CuOEDPxE1? M1X1UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUe2OlM{Lz5{OEW3OlY{OzxxYU6=
MIAPaCa2 M{TLTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWHnbVB[PDhiaILz Mn7mS2k2OCB;IEO1Mlg{KM7:TR?= NIS0eIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-JNK / JNK / p-ATF2 / p-c-Jun; 

PubMed: 16307741     


Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h.

caspase-3 / caspase-8 / caspase-9; 

PubMed: 16307741     


H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control.

pS-ERα / ERα / pERK / ERK; 

PubMed: 27384485     


MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected.

LC3-I / LC3-II; 

PubMed: 27384485     


A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h).

p70S6K1 / p-S6 / HIF-1α; 

PubMed: 27765914     


Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control. 

16307741 27384485 27765914
Immunofluorescence
BMI1; 

PubMed: 27177084     


Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining. 

27177084
Growth inhibition assay
Cell viability ; 

PubMed: 27765914     


A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.

27765914
In vivo Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. [2]

Protocol

Cell Research:[2]
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  • Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
  • Concentrations: 0.2 μM
  • Incubation Time: 24 hours or 48 hours
  • Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Athymic nude mice with MIA PaCa-2 cells
  • Dosages: 50 mg/kg or 100 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 19 mg/mL (63.4 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
19mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 299.66
Formula

C9H11F2N3O4.HCI

CAS No. 122111-03-9
Storage powder
in solvent
Synonyms NSC 613327
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04871321 Recruiting Drug: nab-paclitaxel plus gemcitabine-cisplatin Biliary Tract Cancer|Cholangiocarcinoma|Gallbladder Cancer CHA University|Dong-A Pharmaceutical May 3 2021 --
NCT04634539 Recruiting Drug: Gemcitabine|Drug: Nab-paclitaxel|Drug: L-glutamine Advanced Pancreatic Adenocarcinoma|Pancreatic Cancer|Pancreatic Ductal Adenocarcinoma Jun Gong MD|Emmaus Medical Inc.|Cedars-Sinai Medical Center April 2021 Phase 1
NCT04616534 Recruiting Drug: Elimusertib|Drug: Gemcitabine Advanced Fallopian Tube Carcinoma|Advanced Malignant Solid Neoplasm|Advanced Ovarian Carcinoma|Advanced Pancreatic Adenocarcinoma|Advanced Primary Peritoneal Carcinoma|High Grade Ovarian Serous Adenocarcinoma|Platinum-Resistant Fallopian Tube Carcinoma|Platinum-Resistant Ovarian Carcinoma|Platinum-Resistant Primary Peritoneal Carcinoma|Stage II Pancreatic Cancer AJCC v8|Stage IIA Pancreatic Cancer AJCC v8|Stage IIB Pancreatic Cancer AJCC v8|Stage III Fallopian Tube Cancer AJCC v8|Stage III Ovarian Cancer AJCC v8|Stage III Pancreatic Cancer AJCC v8|Stage III Primary Peritoneal Cancer AJCC v8|Stage IIIA Fallopian Tube Cancer AJCC v8|Stage IIIA Ovarian Cancer AJCC v8|Stage IIIA Primary Peritoneal Cancer AJCC v8|Stage IIIA1 Fallopian Tube Cancer AJCC v8|Stage IIIA1 Ovarian Cancer AJCC v8|Stage IIIA2 Fallopian Tube Cancer AJCC v8|Stage IIIA2 Ovarian Cancer AJCC v8|Stage IIIB Fallopian Tube Cancer AJCC v8|Stage IIIB Ovarian Cancer AJCC v8|Stage IIIB Primary Peritoneal Cancer AJCC v8|Stage IIIC Fallopian Tube Cancer AJCC v8|Stage IIIC Ovarian Cancer AJCC v8|Stage IIIC Primary Peritoneal Cancer AJCC v8|Stage IV Fallopian Tube Cancer AJCC v8|Stage IV Ovarian Cancer AJCC v8|Stage IV Pancreatic Cancer AJCC v8|Stage IV Primary Peritoneal Cancer AJCC v8|Stage IVA Fallopian Tube Cancer AJCC v8|Stage IVA Ovarian Cancer AJCC v8|Stage IVA Primary Peritoneal Cancer AJCC v8|Stage IVB Fallopian Tube Cancer AJCC v8|Stage IVB Ovarian Cancer AJCC v8|Stage IVB Primary Peritoneal Cancer AJCC v8 National Cancer Institute (NCI) February 12 2021 Phase 1
NCT04657068 Recruiting Drug: ART0380|Drug: Gemcitabine Advanced Cancer|Metastatic Cancer|Ovarian Cancer Artios Pharma Ltd January 13 2021 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    What’s the difference between S1714 and S1149 and which one is better?

  • Answer:

    They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

DNA/RNA Synthesis Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID