Gemcitabine HCl

For research use only.

Catalog No.S1149 Synonyms: LY188011

90 publications

Gemcitabine HCl Chemical Structure

CAS No. 122111-03-9

Gemcitabine HCl (LY188011) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

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Selleck's Gemcitabine HCl has been cited by 90 publications

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Gemcitabine HCl (LY188011) is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
Features Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.
Targets
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM M{\RbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3X1bmlEPTB;Mj65JOKyKDFwODDuUS=> NUX1c|NQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM/dCK−/− M33BTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHiXGlKSzVyPUK0NE41KMLzIEK5MlAh|ryP M2m2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 wt NGrEcmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXzV5BKSzVyPUGuN{DDuSByLkOgcm0> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
L1210 10K MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfBUnNKSzVyPUKyMlIhyrFiMz63JO69VQ>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
TC-1  NVTJWI1KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTF2LkegxtEhOi56IH7N MnziQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
TC-1-GR MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrZWlRKSzVyPUO2MlchyrFiNT6xJO69VQ>? M1jW[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
MIA PaCa-2 MkK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfr[3BKSzVyPUS5MlchyrFiMUeuO{BvVQ>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
PANC-1 NYX3WndlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;wTWM2OD1-IESwNEDPxE1? MmPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
CCRF-CEM M2\3e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlyyTWM2OD1{LkmgxtEhOS56IH7N NXW0O4pIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
CCRF-CEM-AraC-8C M3jMbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTDflhEUUN3ME25PVgvQCEEsTC5MlQhdk1? M1\IWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM  NHPmRXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHf1Uow4OiCq M{PjbmlEPTB;Mj6wJOKyKDBwNjDuUS=> Ml61QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NUG4OFMoRjJzOEWxPFQ{RC:jPh?=
MV-4-11 M4TMTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVXXb4I4UUN3MDC9JFAvODByNTDuUS=> MkW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ES4 NEexfINIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVzDdnpwUUN3MDC9JFAvODByNzDuUS=> NIfPbVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ACHN M3;RT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlL2TWM2OCB;IECuNFAxQSCwTR?= NELqbmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KYSE-510 MoLuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmfTTWM2OCB;IECuNFAyKG6P MmjDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
EW-7 MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml\WTWM2OCB;IECuNFAzPiCwTR?= NUjteotqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
BFTC-905 NF[yeGNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEDyRVlKSzVyIE2gNE4xODVzIH7N NIP5RpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
KE-37 MlSxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MY\JR|UxKD1iMD6wNFU3KG6P M{jJZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SBC-5 MnXHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYjJR|UxKD1iMD6wNFU4KG6P NETOOZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
NKM-1 MmT3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1XiVmlEPTBiPTCwMlAxPzFibl2= NV;xU|c5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
RH-1 NHnNdJNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX3pR25yUUN3MDC9JFAvODB5MjDuUS=> M1ixd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ALL-PO MmjUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXv5[nFlUUN3MDC9JFAvODB6MzDuUS=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
QIMR-WIL MnS2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV\Ie4liUUN3MDC9JFAvODB6OTDuUS=> NF;BR|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375 NHW4W|RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJR|UxKD1iMD6wNFk6KG6P NEj0NHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SIG-M5 NULTZVlFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkDsTWM2OCB;IECuNFExPCCwTR?= NXW2Wo9RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KGN M{fGNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjJR|UxKD1iMD6wNVA5KG6P MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-13 MmiwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1zvVmlEPTBiPTCwMlAyOTJibl2= M1WyNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
NCI-SNU-1 NH\yVZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3z6[mlEPTBiPTCwMlAyPiCwTR?= M{nvVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
PSN1 MnznS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFnlVmlKSzVyIE2gNE4xOTZ3IH7N MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HUTU-80 NVviNHdET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVXJR|UxKD1iMD6wNVY3KG6P NEnDd5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
786-0 NGLBdlhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkCwTWM2OCB;IECuNFI{KG6P MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
EW-16 MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUC2W3pQUUN3MDC9JFAvODJ|IH7N NEO3ZlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ES1 NGrnRZdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MorkTWM2OCB;IECuNFI3QCCwTR?= MnXhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RKO NFryd|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJR|UxKD1iMD6wNlc5KG6P NIjwNIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ESS-1 NF;hS5FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mnz1TWM2OCB;IECuNFI5PiCwTR?= NHHwTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SK-UT-1 M3zwRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEjEdmVKSzVyIE2gNE4xOjl5IH7N MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
LB2241-RCC M2fvTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3fxfGlEPTBiPTCwMlA{OThibl2= M3PNXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
CHL-1 MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\CRYdKSzVyIE2gNE4xOzJ2IH7N M{Pxd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SW1783 NFLiWopIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX\JR|UxKD1iMD6wN|M3KG6P MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
MEL-JUSO NX;TUphuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4j1emlEPTBiPTCwMlA{QTFibl2= M4nsZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HT-29 NYDUVW5ET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnTzTWM2OCB;IECuNFQyOyCwTR?= Mm\FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SNG-M M1zsUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmTYTWM2OCB;IECuNFQzPSCwTR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
TE-15 NV7HbHAzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmK1TWM2OCB;IECuNFQ3PCCwTR?= NGr2T|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HOS MlX3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYHJR|UxKD1iMD6wOFghdk1? NIPnSVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
BB65-RCC M{LsdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIfxNZJKSzVyIE2gNE4xPTF{IH7N NGfzO5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HCE-4 MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW\XV49HUUN3MDC9JFAvODV{ODDuUS=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
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BL21(DE3) MXvGeY5kfGmxbjDhd5NigQ>? M4HsOWtuKD1iMT60N{DPxE1? NIXJdWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN|AyOzF:L3G+
U87MG M2TXXmN6fG:2b4jpZ4l1gSCjc4PhfS=> NFrZcVE{KGSjeYO= NYO3RZhwUUN3MDC9JFEvPDlizszN M3XhWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
BL21(DE3) MoOxSpVv[3Srb36gZZN{[Xl? MVTLcUA:KDFwNUKg{txO M4iwOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkOwNVMyLz5{M{KzNFE{OTxxYU6=
HT-29 NIK5V5BEgXSxdH;4bYNqfHliYYPzZZk> NXTIZWFTOyCmYYnz MV\JR|UxKD1iMT61N{DPxE1? NWPUfGI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
SW1990 MlWxR5l1d3SxeHnjbZR6KGG|c3H5 NGjKSo9KSzVyIE2gNU43KM7:TR?= M2LNcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUm1OFY3Lz5{NEG5OVQ3PjxxYU6=
Capan2 NXTLbmRqS3m2b4TvfIlkcXS7IHHzd4F6 MYO3NkBpenN? NFnhbJNKSzVyIE2gNU44KM7:TR?= MlvFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
SW480 NF;IVndCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mk\SO|IhcHK| MX7JR|UxKD1iMT63JO69VQ>? M37YOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEezO|QxLz5{MEi3N|c1ODxxYU6=
PANC1 NWnJNnVDS3m2b4TvfIlkcXS7IHHzd4F6 NED5THg4OiCqcoO= NVv6WIRPUUN3MDC9JFEvPyEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ3NkKwNkc,Ojl4NU[yNFI9N2F-
HCT8 NYnrWZh6S3m2b4TvfIlkcXS7IHHzd4F6 M1PQdVczKGi{cx?= MmPpTWM2OCB;IEGuO|Qh|ryP Mo\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
BL21(DE3) MnLYSpVv[3Srb36gZZN{[Xl? M3nQeGtuKD1iMT63OUDPxE1? NGnIS|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN|AyOzF:L3G+
HT-29 M2PCZWN6fG:2b4jpZ4l1gSCjc4PhfS=> MoHFOFghcHK| NYLBVHNsUUN3MDC9JFEvQTVizszN MlHZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ|OU[4PFI1RC:jPh?=
MIAPaCa2 M4PhbmN6fG:2b4jpZ4l1gSCjc4PhfS=> NIHoeoI2KGSjeYO= NIW2NlRGSzVyIE2gNkDPxE1? M{XJW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OE[3OVkxLz5{NEi2O|U6ODxxYU6=
BL21(DE3) MVXGeY5kfGmxbjDhd5NigQ>? NV[4RlY1OSCvaX6= NFG0N2hMdSB;IEKuNVUh|ryP Mm\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
SW1990 MoK3R5l1d3SxeHnjbZR6KGG|c3H5 M1G5XmlEPTBiPTCyMlIh|ryP M3XtU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUC1O|IzLz5{NUGwOVczOjxxYU6=
SW1990 MXHDfZRwfG:6aXPpeJkh[XO|YYm= NVfuPINbPDhiaILz NYnJPWxOUUN3MDC9JFIvOyEQvF2= NUnRR|ZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke5OlY6PTBpPkK3PVY3QTVyPD;hQi=>
NCI-H146 M2jSS2N6fG:2b4jpZ4l1gSCjc4PhfS=> MVGzJIRigXN? MV;JR|UxKD1iMj63PEDPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
BxPC3 M2\pT2N6fG:2b4jpZ4l1gSCjc4PhfS=> MUK3NkBpenN? M4frTmlEPTBiPTCyMlkh|ryP M3y1RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
p53 deficient COLO205 M3H2emFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NFLJTXo4OiCqcoO= M3POTmlEPTBiPTCzJO69VQ>? NGryNHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N|c1OCd-MkC4O|M4PDB:L3G+
MiaPaCa NVLEc|ByS3m2b4TvfIlkcXS7IHHzd4F6 Ml25OkBl[Xm| MWfJR|UxKD1iMzFOwG0> NIHrVW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O2NFExPCd-MkOzOlAyODR:L3G+
COLO205 Mlz2R5l1d3SxeHnjbZR6KGG|c3H5 NIfqOGQ1QCCqcoO= NWG1XoxJUUN3MDC9JFMvOjRizszN M17VVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OU[4PFI1Lz5{M{m2PFgzPDxxYU6=
MCF7 MX7DfZRwfG:6aXPpeJkh[XO|YYm= MnzjO|IhcHK| NF7IWmtKSzVyIE2gN{4zQCEQvF2= M372RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
MIAPaCa2 MmTER5l1d3SxeHnjbZR6KGG|c3H5 NET4TWE4OiCqcoO= MYTJR|UxKD1iMz6zJO69VQ>? M17sfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{K4OlU3Lz5{OUOyPFY2PjxxYU6=
HeLa NH62c4ZEgXSxdH;4bYNqfHliYYPzZZk> M{fVdFI1KGi{cx?= NXHGXng6UUN3MDC9JFMvOyEQvF2= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyM{K5Okc,OjV5MEOyPVY9N2F-
FTC-133 NInXb5JEgXSxdH;4bYNqfHliYYPzZZk> MUmyOEBpenN? M4jQZ2lEPTBiPTCzMlM3KM7:TR?= MnPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3 NXLOPVZiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\vSGV3PDhiaILz NHr3dGVIUTVyIE2gN{43PCEQvF2= NGK0Omg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+
COLO320DM Mmm1R5l1d3SxeHnjbZR6KGG|c3H5 NIDLbFY1QCCqcoO= M4qwNGlEPTBiPTCzMlkzKM7:TR?= MnnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ|OU[4PFI1RC:jPh?=
HeLa NXr0Z3lLS3m2b4TvfIlkcXS7IHHzd4F6 MXSzJIRigXN? NHXEeYdKSzVyIE2gOE4yOiEQvF2= M1\nVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
8305C NVe5RmwxS3m2b4TvfIlkcXS7IHHzd4F6 M4HpRVI1KGi{cx?= MUTJR|UxKD1iND61N{DPxE1? NUizOo1LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N|Y6QTRpPkK0OFM3QTl2PD;hQi=>
PANC1 NEK3em9EgXSxdH;4bYNqfHliYYPzZZk> NHLqTIs4OiCqcoO= NEPISoNKSzVyIE2gOU43KM7:TR?= NUPCPWhQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
PANC1 MnLoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGPJNGE4OiCqcoO= NYW2PFRTT0l3MDC9JFUvQCEQvF2= NX\rNIF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0PVUxQDFpPkK4OFk2ODhzPD;hQi=>
SK-MEL-2 MXrDfZRwfG:6aXPpeJkh[XO|YYm= NHPxZm8{KGSjeYO= MWfJR|UxKD1iNz6xNUDPxE1? M2LQV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
CEM MmDWR5l1d3SxeHnjbZR6KGG|c3H5 NV3QVZdIOyCmYYnz M1rp[mlEPTBiPTC3MlYh|ryP MoDNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEGzOVYoRjJ2M{SxN|U3RC:jPh?=
SW1573 NFTjWZBEgXSxdH;4bYNqfHliYYPzZZk> MnLqO|IhcHK| M{T5SWlEPTBiPTC4MlMh|ryP NXrUeIlKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVk3ODRpPkG3OFE6PjB2PD;hQi=>
BxPC3 M1fzNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUTyemVHPDhiaILz MWTMR|UxKD1iOD63NUDPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVzMkmwPEc,OjJ3MUK5NFg9N2F-
MCF7 Ml;iR5l1d3SxeHnjbZR6KGG|c3H5 MXW3NkBpenN? MXLJR|UxKD1iOD65JO69VQ>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB5NUW5Nkc,OjhyN{W1PVI9N2F-
BJ MUXDfZRwfG:6aXPpeJkh[XO|YYm= M{HqN|Mh\GG7cx?= NIn2cYpKSzVyIE2gPU45QCEQvF2= NFe3TIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
HeLa NHiybWJEgXSxdH;4bYNqfHliYYPzZZk> NYP5dos6PzJiaILz M1T1WmlEPTBiPTCxNEDPxE1? MmrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{GzOVkoRjJ2NkOxN|U6RC:jPh?=
B16-F10-Luc-G5 M4XMN2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnOwNlQhcHK| NUTTW4NYUUN3MDC9JFEyKM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN2OUOzNkc,Ojd|NEmzN|I9N2F-
HCT15 NVzyeWtES3m2b4TvfIlkcXS7IHHzd4F6 NGC3[2VKSzVyIE2gNVEvQCEQvF2= NUXv[5hHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixPFY3ODRpPkG4NVg3PjB2PD;hQi=>
A549 MmXPR5l1d3SxeHnjbZR6KGG|c3H5 MXq3NkBpenN? MXXJR|UxKD1iMUOuNUDPxE1? M2fFdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
HCT116 NXLQVlczS3m2b4TvfIlkcXS7IHHzd4F6 M1LvU2lEPTBiPTCxOE4{KM7:TR?= NETBc5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG4OlYxPCd-MUixPFY3ODR:L3G+
MiaPaCa MVjDfZRwfG:6aXPpeJkh[XO|YYm= NVL1NHRmPzJiaILz M1i0bmlEPTBiPTCxO{4yKM7:TR?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR6OU[yOkc,OjN2OEm2NlY9N2F-
AG6000 NHKyU|BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXPJR|UxKD1iMkCg{txO MlTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
AG6000 NGfHdlJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH;JU25KSzVyIE2gNlAh|ryP MnruQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
BxPC3 Mm[zRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXvGU2RVPzJiaILz MlnCTWM2OCB;IEKwMlIh|ryP NVrlRnZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O|Y3OzNpPkK4OVc3PjN|PD;hQi=>
CV1 NUDRZ|B[S3m2b4TvfIlkcXS7IHHzd4F6 MmfiO|IhcHK| NH70fJBKSzVyIE2gNlEvQSEQvF2= MkXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEm2NlYoRjJ|NEi5OlI3RC:jPh?=
AsPC1 M2j5O2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUW3NkBpenN? NXnvenZIUUN3MDC9JFI3NjhizszN NI\Ccnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
PANC1 M{fZZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUS3NkBpenN? MYLJR|UxKD1iM{CuOEDPxE1? NH;qO489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
MIAPaCa2 MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEDzfGU1QCCqcoO= NIfiRWlIUTVyIE2gN|UvQDNizszN MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVzMkmwPEc,OjJ3MUK5NFg9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p70S6K1 / p-S6 / HIF-1α; 

PubMed: 27765914     


Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control. 

LC3-I / LC3-II; 

PubMed: 27384485     


A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h).

pS-ERα / ERα / pERK / ERK; 

PubMed: 27384485     


MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected.

caspase-3 / caspase-8 / caspase-9; 

PubMed: 16307741     


H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control.

p-JNK / JNK / p-ATF2 / p-c-Jun; 

PubMed: 16307741     


Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h.

27765914 27384485 16307741
Immunofluorescence
BMI1; 

PubMed: 27177084     


Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining. 

27177084
Growth inhibition assay
Cell viability ; 

PubMed: 27765914     


A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.

27765914
In vivo Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. [2]

Protocol

Cell Research:[2]
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  • Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
  • Concentrations: 0.2 μM
  • Incubation Time: 24 hours or 48 hours
  • Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Athymic nude mice with MIA PaCa-2 cells
  • Dosages: 50 mg/kg or 100 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 19 mg/mL (63.4 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
19mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 299.66
Formula

C9H11F2N3O4.HCI

CAS No. 122111-03-9
Storage powder
in solvent
Synonyms LY188011
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04634539 Not yet recruiting Drug: Gemcitabine|Drug: Nab-paclitaxel|Drug: L-glutamine Advanced Pancreatic Adenocarcinoma|Pancreatic Cancer|Pancreatic Ductal Adenocarcinoma Jun Gong MD|Emmaus Medical Inc.|Cedars-Sinai Medical Center January 1 2021 Phase 1
NCT04383119 Not yet recruiting Drug: Trabectedin|Drug: Gemcitabine Leiomyosarcoma of Ovary|Soft Tissue Sarcoma Italian Sarcoma Group|PharmaMar January 15 2021 Phase 2
NCT04616534 Not yet recruiting Drug: Elimusertib|Drug: Gemcitabine Advanced Fallopian Tube Carcinoma|Advanced Malignant Solid Neoplasm|Advanced Ovarian Carcinoma|Advanced Pancreatic Adenocarcinoma|Advanced Primary Peritoneal Carcinoma|High Grade Ovarian Serous Adenocarcinoma|Platinum-Resistant Fallopian Tube Carcinoma|Platinum-Resistant Ovarian Carcinoma|Platinum-Resistant Primary Peritoneal Carcinoma|Stage II Pancreatic Cancer AJCC v8|Stage IIA Pancreatic Cancer AJCC v8|Stage IIB Pancreatic Cancer AJCC v8|Stage III Fallopian Tube Cancer AJCC v8|Stage III Ovarian Cancer AJCC v8|Stage III Pancreatic Cancer AJCC v8|Stage III Primary Peritoneal Cancer AJCC v8|Stage IIIA Fallopian Tube Cancer AJCC v8|Stage IIIA Ovarian Cancer AJCC v8|Stage IIIA Primary Peritoneal Cancer AJCC v8|Stage IIIA1 Fallopian Tube Cancer AJCC v8|Stage IIIA1 Ovarian Cancer AJCC v8|Stage IIIA2 Fallopian Tube Cancer AJCC v8|Stage IIIA2 Ovarian Cancer AJCC v8|Stage IIIB Fallopian Tube Cancer AJCC v8|Stage IIIB Ovarian Cancer AJCC v8|Stage IIIB Primary Peritoneal Cancer AJCC v8|Stage IIIC Fallopian Tube Cancer AJCC v8|Stage IIIC Ovarian Cancer AJCC v8|Stage IIIC Primary Peritoneal Cancer AJCC v8|Stage IV Fallopian Tube Cancer AJCC v8|Stage IV Ovarian Cancer AJCC v8|Stage IV Pancreatic Cancer AJCC v8|Stage IV Primary Peritoneal Cancer AJCC v8|Stage IVA Fallopian Tube Cancer AJCC v8|Stage IVA Ovarian Cancer AJCC v8|Stage IVA Primary Peritoneal Cancer AJCC v8|Stage IVB Fallopian Tube Cancer AJCC v8|Stage IVB Ovarian Cancer AJCC v8|Stage IVB Primary Peritoneal Cancer AJCC v8 National Cancer Institute (NCI) December 31 2020 Phase 1
NCT04657068 Not yet recruiting Drug: ART0380|Drug: Gemcitabine Advanced Cancer|Metastatic Cancer|Ovarian Cancer Artios Pharma Ltd December 2020 Phase 1|Phase 2
NCT04640623 Not yet recruiting Drug: TAR-200|Drug: Cetrelimab Urinary Bladder Neoplasms Janssen Research & Development LLC December 17 2020 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    What’s the difference between S1714 and S1149 and which one is better?

  • Answer:

    They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

DNA/RNA Synthesis Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID