Gemcitabine (LY-188011) HCl

Catalog No.S1149 Synonyms: NSC 613327

For research use only.

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Gemcitabine (LY-188011) HCl Chemical Structure

CAS No. 122111-03-9

Selleck's Gemcitabine (LY-188011) HCl has been cited by 144 publications

Purity & Quality Control

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Biological Activity

Description Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
Features Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.
Targets
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
In vitro

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM NYfGVnl5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjZXmdWUUN3ME2yMlkhyrFiMT64JI5O NEfCXZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM/dCK−/− NXqw[4pDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzKSWI1UUN3ME2yOFAvPCEEsTCyPU4xKM7:TR?= NX:yR3c5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 wt MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TBPGlEPTB;MT6zJOKyKDBwMzDuUS=> M2fB[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 10K NGPyfFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1i5TmlEPTB;MkKuNkDDuSB|Lkeg{txO MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
TC-1  MmXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPXZVRZUUN3ME2xOE44KMLzIEKuPEBvVQ>? NUf4NXp[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
TC-1-GR M1Pyfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILobnJKSzVyPUO2MlchyrFiNT6xJO69VQ>? MnjUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
MIA PaCa-2 NHrpS4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnTTWM2OD12OT63JOKyKDF5Lkegcm0> NGDT[289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
PANC-1 M2LaPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17YNGlEPTB;PjC0NFAh|ryP NF[1dZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM NVX1[nJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLLTWM2OD1{LkmgxtEhOS56IH7N MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM-AraC-8C NFfmTI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HWbWlEPTB;OUm4MlghyrFiOT60JI5O M3nz[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM  NG\a[5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\1O|IhcA>? M13BOWlEPTB;Mj6wJOKyKDBwNjDuUS=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUi0N{c,OjF6NUG4OFM9N2F-
MV-4-11 NH72XWFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHfQeHJKSzVyIE2gNE4xODB3IH7N NXmwTYhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ES4 NUHm[5R6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYfJR|UxKD1iMD6wNFA4KG6P NFL2b4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ACHN MmO3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUnJR|UxKD1iMD6wNFA6KG6P MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
KYSE-510 MlLwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4HGW2lEPTBiPTCwMlAxOSCwTR?= M3TYU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
EW-7 NWKwUmN[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4CwRWlEPTBiPTCwMlAxOjZibl2= Mkj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
BFTC-905 MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1nqdGlEPTBiPTCwMlAxPTFibl2= NY\SU3VIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KE-37 MlXOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYrJR|UxKD1iMD6wNFU3KG6P M2D6dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SBC-5 M{DrUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFvKfZJKSzVyIE2gNE4xODV5IH7N NX3GVGpKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NKM-1 NWf3VIJ{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NULJ[5JVUUN3MDC9JFAvODB5MTDuUS=> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
RH-1 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmLKTWM2OCB;IECuNFA4OiCwTR?= NV22dXhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ALL-PO M2D2eGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYjJR|UxKD1iMD6wNFg{KG6P NETU[VQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
QIMR-WIL MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVjJR|UxKD1iMD6wNFg6KG6P NH\zcJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375 M2S3SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEDqXmdKSzVyIE2gNE4xODl7IH7N M2PiOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SIG-M5 NEHrPVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVTJR|UxKD1iMD6wNVA1KG6P NWHxNXZ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KGN NUnYboV1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGfnW|RKSzVyIE2gNE4xOTB6IH7N NHPxdoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
EW-13 NE\4SlNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEXtbHZKSzVyIE2gNE4xOTF{IH7N MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
NCI-SNU-1 NXrQSHZGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXjiU|RrUUN3MDC9JFAvODF4IH7N NWq1ZnN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
PSN1 M33wfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFj4TYZKSzVyIE2gNE4xOTZ3IH7N MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HUTU-80 M2jmRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXricZdDUUN3MDC9JFAvODF4NjDuUS=> NUHiR5ZLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
786-0 NEfOUJZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3:4fmlEPTBiPTCwMlAzOyCwTR?= NYHMRWpURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-16 NVTUW25QT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHTQZotKSzVyIE2gNE4xOjNibl2= MofCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ES1 MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MY\JR|UxKD1iMD6wNlY5KG6P MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
RKO NH\CeZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYf6e5pZUUN3MDC9JFAvODJ5ODDuUS=> NWTzPGtxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ESS-1 MlfsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIC0c3pKSzVyIE2gNE4xOjh4IH7N Mnr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SK-UT-1 MoDRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4DaUmlEPTBiPTCwMlAzQTdibl2= MoGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
LB2241-RCC M1XCTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYPKcXJ{UUN3MDC9JFAvODNzODDuUS=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
CHL-1 M2rLRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYjUbIhnUUN3MDC9JFAvODN{NDDuUS=> M1TXSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SW1783 MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3frOmlEPTBiPTCwMlA{OzZibl2= NHnSUYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
MEL-JUSO MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYHlXJJMUUN3MDC9JFAvODN7MTDuUS=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HT-29 NHvB[nRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnjTWM2OCB;IECuNFQyOyCwTR?= NEKxdXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SNG-M NG\yZWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4rVVWlEPTBiPTCwMlA1OjVibl2= NVvC[GZ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
TE-15 M1XFcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWHJR|UxKD1iMD6wOFY1KG6P M{DGXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HOS M33LVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYjRTndLUUN3MDC9JFAvODR6IH7N NFv6b5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
BB65-RCC NVjXfos4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJR|UxKD1iMD6wOVEzKG6P MkPoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
HCE-4 NFPQcFdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmT5TWM2OCB;IECuNFUzQCCwTR?= MkXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
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MIAPaCa2 M3T5c2N6fG:2b4jpZ4l1gSCjc4PhfS=> M171S|czKGi{cx?= NHW4bIFKSzVyIE2gNU4xPCEQvF2= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5MUm5PEc,OjR2N{G5PVg9N2F-
SK-N-AS NXXl[W8yS3m2b4TvfIlkcXS7IHHzd4F6 MnfZN{Bl[Xm| NX30VY1VUUN3MDC9JFEvOSEQvF2= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
SW1990 M3XPT2N6fG:2b4jpZ4l1gSCjc4PhfS=> MV23NkBpenN? M1LadmlEPTBiPTCxMlIh|ryP NXPZVG93RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
A549 MW\DfZRwfG:6aXPpeJkh[XO|YYm= NInwNoQ4OiCqcoO= MWTJR|UxKD1iMT60JO69VQ>? M{GzSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
HepG2 NIL2TXhHfW6ldHnvckBie3OjeR?= Ml\oT40hRSBzLkSg{txO NHH1fJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GwNVY4PCd-MUexNFE3PzR:L3G+
BL21(DE3) NVfGcpV{TnWwY4Tpc44h[XO|YYm= NYXnfFNRU21iPTCxMlQ{KM7:TR?= MnLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
U87MG M4DYSmN6fG:2b4jpZ4l1gSCjc4PhfS=> M3\NV|Mh\GG7cx?= NX3iRoNxUUN3MDC9JFEvPDlizszN NFPBZ5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
BL21(DE3) MnvBSpVv[3Srb36gZZN{[Xl? M4\PNGtuKD1iMT61NkDPxE1? NHLRdG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNFE{OSd-MkOyN|AyOzF:L3G+
HT-29 NF\ITHBEgXSxdH;4bYNqfHliYYPzZZk> MX2zJIRigXN? MmfPTWM2OCB;IEGuOVMh|ryP M3XUV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990 NXS2V5VIS3m2b4TvfIlkcXS7IHHzd4F6 MXnJR|UxKD1iMT62JO69VQ>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF7NUS2Okc,OjRzOUW0OlY9N2F-
Capan2 MlnDR5l1d3SxeHnjbZR6KGG|c3H5 NIq2TI84OiCqcoO= NGfCbXlKSzVyIE2gNU44KM7:TR?= NHPmZZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
SW480 NVL0U5M6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUXYe29YPzJiaILz MV3JR|UxKD1iMT63JO69VQ>? M1nmWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEezO|QxLz5{MEi3N|c1ODxxYU6=
PANC1 NH[zUpZEgXSxdH;4bYNqfHliYYPzZZk> Mkm3O|IhcHK| M2rTU2lEPTBiPTCxMlch|ryP NYP0S|QyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OVYzODJpPkK5OlU3OjB{PD;hQi=>
HCT8 Mlu3R5l1d3SxeHnjbZR6KGG|c3H5 MXy3NkBpenN? M3P2O2lEPTBiPTCxMlc1KM7:TR?= NVu0ZmE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
BL21(DE3) M{HDWmZ2dmO2aX;uJIF{e2G7 Moq3T40hRSBzLke1JO69VQ>? NVT3bFlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|AyOzFpPkKzNlMxOTNzPD;hQi=>
HT-29 Mn;UR5l1d3SxeHnjbZR6KGG|c3H5 M4P0PVQ5KGi{cx?= NF\uRnNKSzVyIE2gNU46PSEQvF2= Mnv5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ|OU[4PFI1RC:jPh?=
MIAPaCa2 NUC4WnF2S3m2b4TvfIlkcXS7IHHzd4F6 MX[1JIRigXN? MVnFR|UxKD1iMjFOwG0> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh4N{W5NEc,OjR6Nke1PVA9N2F-
BL21(DE3) MoXkSpVv[3Srb36gZZN{[Xl? NUL5UGpVOSCvaX6= M4rQTWtuKD1iMj6xOUDPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ|MEGzNUc,OjN{M{CxN|E9N2F-
SW1990 MUHDfZRwfG:6aXPpeJkh[XO|YYm= MkD2TWM2OCB;IEKuNkDPxE1? Mlj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEW3NlIoRjJ3MUC1O|IzRC:jPh?=
SW1990 MmjhR5l1d3SxeHnjbZR6KGG|c3H5 NF;YUXI1QCCqcoO= M2PwcmlEPTBiPTCyMlMh|ryP M2T0WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OU[2PVUxLz5{N{m2Olk2ODxxYU6=
NCI-H146 MXjDfZRwfG:6aXPpeJkh[XO|YYm= MmrzN{Bl[Xm| MYPJR|UxKD1iMj63PEDPxE1? MkPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
BxPC3 MonRR5l1d3SxeHnjbZR6KGG|c3H5 M{OzT|czKGi{cx?= NVXZSoVrUUN3MDC9JFIvQSEQvF2= MofXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
p53 deficient COLO205 NX74[GFqSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NVLFN3RmPzJiaILz M{XxZmlEPTBiPTCzJO69VQ>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5M{e0NEc,OjB6N{O3OFA9N2F-
MiaPaCa NH\4WnlEgXSxdH;4bYNqfHliYYPzZZk> NH;ybmw3KGSjeYO= M1TicGlEPTBiPTCzJO69VQ>? NEnJNnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O2NFExPCd-MkOzOlAyODR:L3G+
COLO205 M1XkPWN6fG:2b4jpZ4l1gSCjc4PhfS=> MljCOFghcHK| M1L2S2lEPTBiPTCzMlI1KM7:TR?= NIi4dGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MCF7 MkXHR5l1d3SxeHnjbZR6KGG|c3H5 MVm3NkBpenN? NV;KdGlxUUN3MDC9JFMvOjhizszN MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl|NkK0O|Q9N2F-
MIAPaCa2 NYPUflAzS3m2b4TvfIlkcXS7IHHzd4F6 NGTaVo44OiCqcoO= MVfJR|UxKD1iMz6zJO69VQ>? M2rqWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{K4OlU3Lz5{OUOyPFY2PjxxYU6=
HeLa NXPqdJFsS3m2b4TvfIlkcXS7IHHzd4F6 NIrEb5AzPCCqcoO= MUXJR|UxKD1iMz6zJO69VQ>? MoPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEOyPVYoRjJ3N{CzNlk3RC:jPh?=
FTC-133 NEfrRm1EgXSxdH;4bYNqfHliYYPzZZk> NXjKcIFXOjRiaILz MXLJR|UxKD1iMz6zOkDPxE1? Mo\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3 NXLnfJZtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M17QelQ5KGi{cx?= MXnHTVUxKD1iMz62OEDPxE1? NWLVS4l1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NVI6ODhpPkKyOVEzQTB6PD;hQi=>
COLO320DM NVznbWpNS3m2b4TvfIlkcXS7IHHzd4F6 MmfkOFghcHK| M363SGlEPTBiPTCzMlkzKM7:TR?= MnLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7Nki4NlQoRjJ|OU[4PFI1RC:jPh?=
HeLa NGXu[4NEgXSxdH;4bYNqfHliYYPzZZk> MlzMN{Bl[Xm| MYHJR|UxKD1iND6xNkDPxE1? M3uwblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
8305C M13adGN6fG:2b4jpZ4l1gSCjc4PhfS=> NYXyeVFXOjRiaILz MlnDTWM2OCB;IESuOVMh|ryP NXjweVhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N|Y6QTRpPkK0OFM3QTl2PD;hQi=>
PANC1 MVXDfZRwfG:6aXPpeJkh[XO|YYm= Ml;KO|IhcHK| NYjvWYZ[UUN3MDC9JFUvPiEQvF2= NWnHNHBYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
PANC1 NFLBWGlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXLhb5Z1PzJiaILz NFnONHhIUTVyIE2gOU45KM7:TR?= NV[4UJQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0PVUxQDFpPkK4OFk2ODhzPD;hQi=>
SK-MEL-2 NGnSeXBEgXSxdH;4bYNqfHliYYPzZZk> NEjOZWs{KGSjeYO= NIj2NpJKSzVyIE2gO{4yOSEQvF2= Mn7sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
CEM NX65U5RkS3m2b4TvfIlkcXS7IHHzd4F6 MnnqN{Bl[Xm| NUS1WGRHUUN3MDC9JFcvPiEQvF2= MoraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEGzOVYoRjJ2M{SxN|U3RC:jPh?=
SW1573 MWjDfZRwfG:6aXPpeJkh[XO|YYm= MXy3NkBpenN? Mn:0TWM2OCB;IEiuN{DPxE1? MoT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUm2NFQoRjF5NEG5OlA1RC:jPh?=
BxPC3 NYfOW4NqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NG\XVXI1QCCqcoO= MVHMR|UxKD1iOD63NUDPxE1? NFXXPFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+
MCF7 NUD3d2NKS3m2b4TvfIlkcXS7IHHzd4F6 MknEO|IhcHK| NGW4fnJKSzVyIE2gPE46KM7:TR?= NVvlWYtbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwO|U2QTJpPkK4NFc2PTl{PD;hQi=>
BJ MXnDfZRwfG:6aXPpeJkh[XO|YYm= NYr6OWcyOyCmYYnz NX;Lbo9XUUN3MDC9JFkvQDhizszN MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
HeLa NGPnSGVEgXSxdH;4bYNqfHliYYPzZZk> Mlf1O|IhcHK| MlvPTWM2OCB;IEGwJO69VQ>? NXfhblZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|E{PTlpPkK0OlMyOzV7PD;hQi=>
B16-F10-Luc-G5 M3H6PWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M{LSSVI1KGi{cx?= NFr1bFZKSzVyIE2gNVEh|ryP NGj2RVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O0PVM{Oid-MkezOFk{OzJ:L3G+
HCT15 M3P0WmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2fCUmlEPTBiPTCxNU45KM7:TR?= NV;DNGNIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixPFY3ODRpPkG4NVg3PjB2PD;hQi=>
A549 Mn7LR5l1d3SxeHnjbZR6KGG|c3H5 M1vPOFczKGi{cx?= NXezWFdSUUN3MDC9JFE{NjFizszN MkLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUm2NFQoRjF5NEG5OlA1RC:jPh?=
HCT116 NEmw[4JEgXSxdH;4bYNqfHliYYPzZZk> NED5N|JKSzVyIE2gNVQvOyEQvF2= MnrjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzOE[2NFQoRjF6MUi2OlA1RC:jPh?=
MiaPaCa M{e0OmN6fG:2b4jpZ4l1gSCjc4PhfS=> NVjTOnYzPzJiaILz M2DJZWlEPTBiPTCxO{4yKM7:TR?= MkTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEm2NlYoRjJ|NEi5OlI3RC:jPh?=
AG6000 NHTaU5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlPaTWM2OCB;IEKwJO69VQ>? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZyMkS2OEc,OTd4MEK0OlQ9N2F-
AG6000 NV3qdpRCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnzKTWM2OCB;IEKwJO69VQ>? NWDrS|NWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
BxPC3 M{fJV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NEPQdmw4OiCqcoO= NIDDUZhKSzVyIE2gNlAvOiEQvF2= NFzFUZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
CV1 MVvDfZRwfG:6aXPpeJkh[XO|YYm= NUPIUldjPzJiaILz NH;WcHdKSzVyIE2gNlEvQSEQvF2= M2nIclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEi5OlI3Lz5{M{S4PVYzPjxxYU6=
AsPC1 NFfTUpRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NGSxbnI4OiCqcoO= MoDmTWM2OCB;IEK2Mlgh|ryP NIm1bG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
PANC1 M{LjbWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M{fhT|czKGi{cx?= NGHhUI1KSzVyIE2gN|AvPCEQvF2= NV\XcZZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O|Y3OzNpPkK4OVc3PjN|PD;hQi=>
MIAPaCa2 NHHrbY1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV[0PEBpenN? NVK0eZZ2T0l3MDC9JFM2Njh|IN88US=> Ml\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3MUK5NFgoRjJ{NUGyPVA5RC:jPh?=
Assay
Methods Test Index PMID
Western blot p-JNK / JNK / p-ATF2 / p-c-Jun ; caspase-3 / caspase-8 / caspase-9 ; pS-ERα / ERα / pERK / ERK ; LC3-I / LC3-II ; p70S6K1 / p-S6 / HIF-1α 16307741 27384485 27765914
Immunofluorescence BMI1 27177084
Growth inhibition assay Cell viability 27765914
In vivo Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. [2]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: BxPC-3, MIA PaCa-2, and PANC-1 cells
  • Concentrations: 0.2 μM
  • Incubation Time: 24 hours or 48 hours
  • Method: BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
Animal Research:[2]
  • Animal Models: Athymic nude mice with MIA PaCa-2 cells
  • Dosages: 50 mg/kg or 100 mg/kg
  • Administration: Administered via i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.

19mg/mL

Chemical Information

Molecular Weight 299.66
Formula

C9H11F2N3O4.HCI

CAS No. 122111-03-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05141149 Not yet recruiting Drug: PBP1510 (400mg/16mL)|Drug: Gemcitabine (1000 mg/m^2) Pancreatic Cancer Prestige Biopharma Limited July 2022 Phase 1|Phase 2
NCT05181462 Recruiting Drug: Carboplatin|Drug: Gemcitabine|Drug: Nadunolimab Triple Negative Breast Cancer Cantargia AB|Spanish Breast Cancer Research Group January 11 2022 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
What’s the difference between S1714 and S1149 and which one is better?

Answer:
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

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