Ligustrazine hydrochloride

Catalog No.S3775 Batch:S377501

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Technical Data

Formula

C8H12N2.HCl
Molecular Weight 172.66 CAS No. 76494-51-4
Solubility (25°C)* In vitro DMSO 34 mg/mL (196.91 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Ligustrazine hydrochloride (Tetramethylpyrazine, Tetrapyrazine) is a chemical compound found in natto and in fermented cocoa beans with anti-inflammation, antioxidant, antiplatelet, and antiapoptosis activities.
In vitro Tetramethylpyrazine (TMP) is described as "calcium antagonist" and produces a vasodilation effect via inhibiting Ca2+ influx and the release of intracellular Ca2+ at first. TMP can affect the calcium influx, at least partly, by mediating the opening of potassium channel. TMP is also reported to have a direct effect on L-type calcium current, since it can reduce calcium transient in a dose-dependent manner when applied to rabbit ventricular myocyte. TMP also exerts an endothelium protective property via downregulating the expression of ICAM-1 and HSP60. TMP can restrain LPS-induced IL-8 overexpression in HUVECs at both the protein and mRNA levels, which is possibly due to blocking the activation of the NF-kB-dependent pathway; the involvement of ERK and p38 MAPK signaling pathway has also been observed[1].
In vivo Tetramethylpyrazine (TMP) can stimulate NO production in pulmonary arteries of rat. Akt and the endothelial isoform of nitric oxide synthase (eNOS) phosphorylation were significantly upregulated after TMP pretreatment in vivo. TMP can suppress the proliferation of VSMC in rabbit aortic vascular. TMP can decrease the ANP mRNA expression in cardiomyocyte hypertrophy rat model and suppress the level of pJAK2, pJAK1, or pSTAT3, demonstrating that TMP can inhibit JAK-STAT signal transduction. It has a short in-vivo half-life (T1/2=2.89 h)[1].

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    HL-60 cells

  • Concentrations

    300 µg/mL

  • Incubation Time

    24, 48, 60, 72 h

  • Method

    Cell viability is determined by the MTT assay. HL-60 cells are treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively.
Animal Study:

[2]
  • Animal Models

    A rat model of Parkinson

  • Dosages

    20 mg/kg/d

  • Administration

    i.p.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.