Ifosfamide (NSC109724)

For research use only.

Catalog No.S1302 Synonyms: Isophosphamide

7 publications

Ifosfamide (NSC109724) Chemical Structure

CAS No. 3778-73-2

Ifosfamide (NSC109724, Isophosphamide) is a nitrogen mustard alkylating agent used in the treatment of cancer.

Selleck's Ifosfamide (NSC109724) has been cited by 7 publications

1 Customer Review

  • Chemotherapeutic response. Ifosfamide was applied at different concentrations on Lipo-DUE1, Lipo246, and PLS-1 for 72 h. Subsequently, cell viability was determined by MTS assay at a wavelength of 490 nm. Values represent the mean±S.D. of triplicates

    Tumour Biol, 2016, 37(2):2341-51. Ifosfamide (NSC109724) purchased from Selleck.

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Biological Activity

Description Ifosfamide (NSC109724, Isophosphamide) is a nitrogen mustard alkylating agent used in the treatment of cancer.
In vitro

Ifosfamide (50 mM) increases CYP3A4, CYP2C8, and CYP2C9 protein levels in hepatocytes, which thereby enhances their own rates of 4-hydroxylation in the cultured hepatocytes. Ifosfamide only induces CYP3A4 in one human hepatocyte culture that contained the polymorphically expressed CYP3A5 in addition to the more widely expressed CYP3A4. [1] Ifosfamide is a prodrug metabolised in the liver by cytochrome P450 mixed-function oxidase enzymes to isofosforamide mustard, the active alkylating compound. Ifosfamide has produced favourable response rates in small cell lung cancer, paediatric solid tumours, non-Hodgkin's and Hodgkin's lymphoma, and ovarian cancer. [2] Ifosfamide is highly cytotoxic to MCF-7 cells following stable transfection of CYP2B1 but exhibits no toxicity to parental tumor cells or to a beta-galactosidase-expressing MCF-7 transfectant, this cytotoxicity could be appreciably blocked by the CYP2B1 inhibitor metyrapone. [3] Ifosfamide combined with Zoledronic acid is more effective than each agent alone in preventing tumor recurrence, improving tissue repair, and increasing bone formation as revealed by the analysis of trabecular architecture. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SJ-GBM2 NIfrd|dyUFSVIHHzd4F6 MmD6dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= NH;YfWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 NXvrWndHeUiWUzDhd5NigQ>? NYLz[lRQeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= M4XEN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=

... Click to View More Cell Line Experimental Data

In vivo Ifosfamide (100 mg/kg, 200 mg/kg and 400 mg/kg) injected intraperitoneally induces a dose dependent increase in bladder wet weight and Evans blue extravasation in mice. Ifosfamide reveals extensive cystitis characterized by acute inflammation with vascular congestion, edema, hemorrhage and fibrin deposition, neutrophil cell infiltration and epithelial denudation in mice. Ifosfamide shows intense reactivity to inducible nitric oxide synthase in the cytoplasm as well as intense and diffuse necrosis on hematoxylin and eosin staining. [5]


Solubility (25°C)

In vitro DMSO 52 mg/mL (199.16 mM)
Water 52 mg/mL (199.16 mM)
Ethanol '52 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 261.09


CAS No. 3778-73-2
Storage powder
in solvent
Synonyms Isophosphamide
Smiles C1CN(P(=O)(OC1)NCCCl)CCCl

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04968106 Not yet recruiting Drug: Doxorubicin|Drug: Ifosfamide|Drug: INCMGA00012 Retroperitoneal Sarcoma|Resectable Sarcoma Institut Bergonié|Incyte Biosciences International Sàrl September 2021 Phase 2
NCT04925089 Recruiting Other: Blood and Tissue collection Leiomyosarcoma Sarcoma Alliance for Research through Collaboration June 2021 --
NCT02432274 Active not recruiting Drug: Lenvatinib|Drug: Ifosfamide|Drug: Etoposide Tumors|Solid Malignant Tumors|Osteosarcoma|Differentiated Thyroid Cancer (DTC) Eisai Limited|Merck Sharp & Dohme Corp.|Eisai Inc. December 29 2014 Phase 1|Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID