Ifosfamide (NSC109724)

Catalog No.S1302 Synonyms: Mitoxana, Ifex,Isophosphamide,NSC109724

For research use only.

Ifosfamide (Mitoxana, Ifex,Isophosphamide,NSC109724) is a nitrogen mustard alkylating agent used in the treatment of cancer.

Ifosfamide (NSC109724) Chemical Structure

CAS No. 3778-73-2

Selleck's Ifosfamide (NSC109724) has been cited by 11 Publications

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Biological Activity

Description Ifosfamide (Mitoxana, Ifex,Isophosphamide,NSC109724) is a nitrogen mustard alkylating agent used in the treatment of cancer.
In vitro

Ifosfamide (50 mM) increases CYP3A4, CYP2C8, and CYP2C9 protein levels in hepatocytes, which thereby enhances their own rates of 4-hydroxylation in the cultured hepatocytes. Ifosfamide only induces CYP3A4 in one human hepatocyte culture that contained the polymorphically expressed CYP3A5 in addition to the more widely expressed CYP3A4. [1] Ifosfamide is a prodrug metabolised in the liver by cytochrome P450 mixed-function oxidase enzymes to isofosforamide mustard, the active alkylating compound. Ifosfamide has produced favourable response rates in small cell lung cancer, paediatric solid tumours, non-Hodgkin's and Hodgkin's lymphoma, and ovarian cancer. [2] Ifosfamide is highly cytotoxic to MCF-7 cells following stable transfection of CYP2B1 but exhibits no toxicity to parental tumor cells or to a beta-galactosidase-expressing MCF-7 transfectant, this cytotoxicity could be appreciably blocked by the CYP2B1 inhibitor metyrapone. [3] Ifosfamide combined with Zoledronic acid is more effective than each agent alone in preventing tumor recurrence, improving tissue repair, and increasing bone formation as revealed by the analysis of trabecular architecture. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SJ-GBM2 NXyxWWM4eUiWUzDhd5NigQ>? NUTyeotteUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM> Mn;1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 MlfkdWhVWyCjc4PhfS=> NHjyeWhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= MoLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
In vivo

Ifosfamide (100 mg/kg, 200 mg/kg and 400 mg/kg) injected intraperitoneally induces a dose dependent increase in bladder wet weight and Evans blue extravasation in mice. Ifosfamide reveals extensive cystitis characterized by acute inflammation with vascular congestion, edema, hemorrhage and fibrin deposition, neutrophil cell infiltration and epithelial denudation in mice. Ifosfamide shows intense reactivity to inducible nitric oxide synthase in the cytoplasm as well as intense and diffuse necrosis on hematoxylin and eosin staining. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 261.09
Formula

C7H15Cl2N2O2P

CAS No. 3778-73-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CN(P(=O)(OC1)NCCCl)CCCl

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04968106 Not yet recruiting Drug: Doxorubicin|Drug: Ifosfamide|Drug: INCMGA00012 Retroperitoneal Sarcoma|Resectable Sarcoma Institut Bergonié|Incyte Biosciences International Sàrl June 1 2022 Phase 2
NCT04925089 Withdrawn Other: Blood and Tissue collection Leiomyosarcoma Sarcoma Alliance for Research through Collaboration June 2021 --
NCT02432274 Active not recruiting Drug: Lenvatinib|Drug: Ifosfamide|Drug: Etoposide Tumors|Solid Malignant Tumors|Osteosarcoma|Differentiated Thyroid Cancer (DTC) Eisai Limited|Merck Sharp & Dohme LLC|Eisai Inc. December 29 2014 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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