IPA-3

Catalog No.S7093

IPA-3 Chemical Structure

Molecular Weight(MW): 350.45

IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).

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Cited by 15 Publications

4 Customer Reviews

  • (e) Immunoblot analysis of p-ERM, p-Pak1 and Pak1 in Ishikawa cells treated with various doses of Pak1 inhibitor IPA-3 for 24 h. Actin serves as a loading control

    Cell Death Differ, 2016, 23(1):169-81. IPA-3 purchased from Selleck.

  • Effect of the PAK1-specific inhibitor IPA-3 on the regulatory role of PTPN12-S19E on EGF-induced cell migration. ***P < 0.001 vs. control; ##P < 0.01 vs. PRPN12-WT.

    FASEB J, 2018, 32(1):73-82. IPA-3 purchased from Selleck.

  • Effect of endocytic pathway inhibitors on the uptake of 0.5 μm and 1 μm beads by IgM+ and IgM− cells in peripheral blood leukocytes (PBL). A. Endocytic rate of IgM+ cells and IgM− cells for 0.5 μm beads in the presence of inhibitors (CPZ, dynasore, M-β-CD, nystatin, IPA-3, and NSC23766). B. Endocytic rate of IgM+ cells and IgM− cells for 1 μm beads in the presence of inhibitors. Data are obtained from three independent experiments. *, P < 0.05; **, P < 0.01.

    Fish Shellfish Immunol, 2019, 84:138-147. IPA-3 purchased from Selleck.

  • The cells were treated with 5 μM of the PAK1-specific inhibitor IPA-3 or 1 μM of the ROCK1/2-specific inhibitor RKI-1447 for 24 h. Total cell lysates were immunoblotted with antibodies against p110β, p110δ, p-PAK1, PAK1, ROCK1, ROCK2, p-LIMK1, LIMK1 or β-actin, which served as an internal control. The results are representative of three independent experiments.

    Anticancer Res, 2017, 37(4):1805-1818. IPA-3 purchased from Selleck.

Purity & Quality Control

Choose Selective PAK Inhibitors

Biological Activity

Description IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).
Features IPA-3 binds covalently to the Pak1 regulatory domain and prevents binding to the upstream activator Cdc42.
Targets
PAK1 [1]
(Cell-free assay)
2.5 μM
In vitro

IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1). PIR3.5 is the control compound of IPA-3. IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts. [1] IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NALM-6 cell M4XwTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXTkOVJUUW6qaXLpeIlwdiCxZjDoeY1idiCQQVzNMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjl5Nkm0JO69VQ>? M1:5NXNCVkeHUh?=
human SW1710 cell NEDWTFFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHy5eWxKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOTd6M{Og{txO NWOwdI1sW0GQR1XS
human YT cell NHLFUlRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUHublRrUW6qaXLpeIlwdiCxZjDoeY1idiC\VDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|kxOzZizszN M{DORnNCVkeHUh?=
human BL-70 cell NHXhSldIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NY\RdIJDUW6qaXLpeIlwdiCxZjDoeY1idiCETD23NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODN5OU[g{txO NWPLfZV6W0GQR1XS
human SIG-M5 cell M2POO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoHrTY5pcWKrdHnvckBw\iCqdX3hckBUUUdvTUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlExOjF7IN88US=> M1jiXHNCVkeHUh?=
human MCF7 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MofSTY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6xPFA4KM7:TR?= MWTTRW5ITVJ?
human GB-1 cell M4rCTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF7GbJdKdmirYnn0bY9vKG:oIHj1cYFvKEeELUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlY{PDl2IN88US=> MkC1V2FPT0WU
human KU812 cell M2LXPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;HVndKdmirYnn0bY9vKG:oIHj1cYFvKEuXOEGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk44PTl3IN88US=> M4Kzc3NCVkeHUh?=
human QIMR-WIL cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUfKOJlNUW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwOUC4PVEh|ryP MXzTRW5ITVJ?
human BL-41 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFrJT5RKdmirYnn0bY9vKG:oIHj1cYFvKEKOLUSxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4xQTh2NDFOwG0> NYfINJJFW0GQR1XS
human CMK cell M4LRZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1;UZ2lvcGmkaYTpc44hd2ZiaIXtZY4hS02NIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6xOFU6QSEQvF2= MYPTRW5ITVJ?
human U-698-M cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV7Jcohq[mm2aX;uJI9nKGi3bXHuJHUuPjl6LV2gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlI{QDV4IN88US=> MmLGV2FPT0WU
human Daoy cell NFrVRlNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGjofY1KdmirYnn0bY9vKG:oIHj1cYFvKESjb4mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlI1PjZ{IN88US=> MnvQV2FPT0WU
human 786-0 cell NHrvcG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIHtOYZKdmirYnn0bY9vKG:oIHj1cYFvKDd6Nj2wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4{OThyNTFOwG0> M2DqTHNCVkeHUh?=
human REH cell M4LiXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2G3bWlvcGmkaYTpc44hd2ZiaIXtZY4hWkWKIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz6zOlY2PSEQvF2= MXvTRW5ITVJ?
human MHH-PREB-1 cell NFXB[WJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoTrTY5pcWKrdHnvckBw\iCqdX3hckBOUEhvUGLFRk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy52Nk[4O{DPxE1? NIfpcIlUSU6JRWK=
human KE-37 cell growth MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHrZeGdKdmirYnn0bY9vKG:oIHj1cYFvKEuHLUO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{42ODJ|MTFOwG0> M{jBW3NCVkeHUh?=
human CAL-51 cell NYjtdVNWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOVQ2OTlizszN M1eyU3NCVkeHUh?=
human CA46 cell M3f0bWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFLkToJKdmirYnn0bY9vKG:oIHj1cYFvKEODNE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlY3OThizszN MnjQV2FPT0WU
human HEL cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4DXcGlvcGmkaYTpc44hd2ZiaIXtZY4hUEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz63Nlc3QCEQvF2= NIDZZ5VUSU6JRWK=
human NB6 cell M1jwbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkP0TY5pcWKrdHnvckBw\iCqdX3hckBPSjZiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkizO|UyKM7:TR?= M120[XNCVkeHUh?=
human 8305C cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWHJcohq[mm2aX;uJI9nKGi3bXHuJFg{ODWFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6xOlc2QCEQvF2= MnX2V2FPT0WU
human EW-13 cell NGHKV3NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUnJcohq[mm2aX;uJI9nKGi3bXHuJGVYNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6yNFE1QCEQvF2= MYPTRW5ITVJ?
human NB13 cell MmrES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4DHNmlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNlI2PiEQvF2= Mn7sV2FPT0WU
human EB2 cell NUDBWlVbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWj5VFJKUW6qaXLpeIlwdiCxZjDoeY1idiCHQkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlQ6OTR{IN88US=> NHXKS2JUSU6JRWK=
human HOS cell NFW1TmpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJGhQWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7 NVnRcG9FW0GQR1XS
human HLE cell NITjRVlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWTpVFNHUW6qaXLpeIlwdiCxZjDoeY1idiCKTFWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlUzOTR7IN88US=> NYLYVpZTW0GQR1XS
human ST486 cell M1XycWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX\o[nhNUW6qaXLpeIlwdiCxZjDoeY1idiCVVES4OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvQDZ3NDFOwG0> NEP5O2ZUSU6JRWK=
human NCI-H2009 cell NX\Xb3FnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV;JfINPUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIxODliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkC0N|U1KM7:TR?= NIHxOnlUSU6JRWK=
human NH-12 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M33TZWlvcGmkaYTpc44hd2ZiaIXtZY4hVkhvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlEyPzZizszN NGn2PWZUSU6JRWK=
human BFTC-905 cell NEflRmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4PDO2lvcGmkaYTpc44hd2ZiaIXtZY4hSk[WQz25NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjF2N{[4JO69VQ>? M3rqSnNCVkeHUh?=
human RKO cell M3TVTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUXue|FqUW6qaXLpeIlwdiCxZjDoeY1idiCUS1:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlI5PDdzIN88US=> NHz3ZnhUSU6JRWK=
human PF-382 NGjmS5BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml\oTY5pcWKrdHnvckBw\iCqdX3hckBRTi1|OEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM2OjJ4IN88US=> NVm5OGVUW0GQR1XS
human SR cell MoPZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXvJcohq[mm2aX;uJI9nKGi3bXHuJHNTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS5|N{K1OkDPxE1? NF3VXIdUSU6JRWK=
human WSU-NHL cell NHuwb5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkfpTY5pcWKrdHnvckBw\iCqdX3hckBYW1VvTljMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU42OjB4NzFOwG0> NGfuUHlUSU6JRWK=
human CAL-12T cell MmXrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlLQTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43PDF2IN88US=> MXvTRW5ITVJ?
human Daudi cell M3G0dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH\4NpNKdmirYnn0bY9vKG:oIHj1cYFvKESjdXTpJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QDR5NzFOwG0> MV;TRW5ITVJ?
human MC-CAR cell M4jnb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWXtXVRuUW6qaXLpeIlwdiCxZjDoeY1idiCPQz3DRXIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjdyNkS0JO69VQ>? Mnf6V2FPT0WU
human RPMI-8402 cell MnXES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mo[xTY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUi0NFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd4OEe2JO69VQ>? MWDTRW5ITVJ?
human CCRF-CEM cell NGj3VldIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlHGTY5pcWKrdHnvckBw\iCqdX3hckBES1KILVPFUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQTd4Nkeg{txO MUXTRW5ITVJ?
human CHP-126 cell M{HIe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWHJcohq[mm2aX;uJI9nKGi3bXHuJGNJWC1zMk[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22Mlc1OjZ6IN88US=> NUDOcFVNW0GQR1XS
human NB69 cell NV7xW411T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKGi3bXHuJG5DPjliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lke2N|UzKM7:TR?= M4DoeHNCVkeHUh?=
human KS-1 cell M2G5cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWDJcohq[mm2aX;uJI9nKGi3bXHuJGtUNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lki4OVQ6KM7:TR?= MV\TRW5ITVJ?
human J82 cell NILjdFhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2f3d2lvcGmkaYTpc44hd2ZiaIXtZY4hUjh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz6wOFk3PSEQvF2= M1TLb3NCVkeHUh?=
human SW872 cell NX\2SGVCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2\UZ2lvcGmkaYTpc44hd2ZiaIXtZY4hW1d6N{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlg{PTV6IN88US=> MlfNV2FPT0WU
human HC-1 cell Ml3ES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVTJcohq[mm2aX;uJI9nKGi3bXHuJGhENTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkC2PFA2KM7:TR?= MofzV2FPT0WU
human DU-145 cell NUHEU21wT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEmxbWhKdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjF2NkSg{txO M2jiT3NCVkeHUh?=
human HL-60 cell M1fk[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIf2OppKdmirYnn0bY9vKG:oIHj1cYFvKEiOLU[wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE45PDZyNTFOwG0> NYfjNFE3W0GQR1XS
human MV-4-11 cell NWHQcJJGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYH4NYx7UW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5zNkS5JO69VQ>? NUTBZ25rW0GQR1XS
human SK-N-DZ cell M1m0[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4XlUGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOjB2MkOg{txO NX[xTmpiW0GQR1XS
human HT-1197 cell NV\DO4MyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWjJcohq[mm2aX;uJI9nKGi3bXHuJGhVNTFzOUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4{ODd|IN88US=> MXHTRW5ITVJ?
human NOS-1 cell NF6wOVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUPJcohq[mm2aX;uJI9nKGi3bXHuJG5QWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuO|E2OiEQvF2= MUXTRW5ITVJ?
human HT-1080 cell MnjUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{O3SGlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUC4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5NjZ{OEOg{txO NHHk[nJUSU6JRWK=
human SK-LU-1 cell M2fOXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYO2SZhrUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OThwOEezNUDPxE1? MUjTRW5ITVJ?
human SW48 cell Mn3tS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF3NVI1KdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNU45QDl5IN88US=> NITYWHRUSU6JRWK=
human A2780 cell NV\EbVY{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGEzPzhyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuN|cyKM7:TR?= NXm4cFRiW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-PAK1 / PAK1 / p-PAK2 / PAK2 / p-Raf1 / Raf1 / p-MEK1 / MEK1 / p-ERK / ERK ; 

PubMed: 30971268     


KYSE30 and KYSE150 cells were treated with indicated concentration of IPA-3 for 48 h, and then the protein levels of p-PAK1 (T423), PAK1, p-PAK2 (S141), PAK2, Raf1, p-Raf1 (S338), MEK1, p-MEK1 (S298), ERK1/2, p-ERK1/2 (T202/Y204) were detected by Western blotting analysis.

30971268
Growth inhibition assay
Cell viability; 

PubMed: 30971268     


The ESCC cells were exposed to increasing concentrations of IPA-3 for 72 h, the cell viability was measured by MTT assay. c-d KYSE30 and KYSE150 cells were exposed to increasing concentrations of IPA-3 for 48 h. 

30971268
Immunofluorescence
Paxillin; 

PubMed: 23894351     


Paxillin protein expression was detected by immunofluorescence analysis under IPA-3 treatment. H2M and H2P (upper panel) cells were serum-starved overnight and treated with either DMSO control or IPA-3 (20 µM) for 15 minutes, followed by FBS replenishment for 10 minutes. Immunofluoresence signals of phalloidin (Red), paxillin (Green) and DAPI (Blue) represent stress fiber, focal adhesion and nucleus, respectively (magnification 40X). The number of focal adhesion (paxillin) were counted in H2M (lower, left panel) and H2P (lower, right panel), and represented in the bar chart. Error bars, mean ± SD of triplicate samples. *P<0.01 (t-test) compared with DMSO control.

NF-κB; 

PubMed: 23894351     


Effect of IPA-3 on subcellular localization of NF-κB was evaluated by immunofluorescence staining. After overnight serum starvation, H2M (left panel) and MIHA (right panel) cells were treated with either DMSO or IPA-3 (20 µM, 15 minutes) followed by an addition of TNF-α (20 ng/ml, 15 minutes). NF-κB was detected with a specific antibody (Green) and nucleus was stained with DAPI (Blue).

BiP; 

PubMed: 24844382     


Cells were pretreated with 50 µM IPA-3 for 30 min and then incubated with Alexa555-labeled SubAB for 1 h at 37 °Cells were imaged by confocal microscopy for colocalization of SubAB (red) with BiP (green) as described in Materials and Methods. Bars represent 20 µm. Experiments were repeated two times with similar results.

23894351 24844382

Protocol

Solubility (25°C)

In vitro DMSO 70 mg/mL (199.74 mM)
Ethanol 7 mg/mL (19.97 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 350.45
Formula

C20H14O2S2

CAS No. 42521-82-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    If this product is cell membrane permeable and would it be suitable for PAK1 inhibition on a pancreatic beta cell line?

  • Answer:

    Based on the reference, I think IPA-3 is able to penetrate the cell membrane, it's likely to inhibit PAK1 on a pancreatic beta cell line although there is no reference confirming it: Figure 3: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963893/; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353635/

PAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID