IPA-3

For research use only.

Catalog No.S7093

35 publications

IPA-3 Chemical Structure

CAS No. 42521-82-4

IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).

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Selleck's IPA-3 has been cited by 35 publications

Purity & Quality Control

Choose Selective PAK Inhibitors

Biological Activity

Description IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).
Features IPA-3 binds covalently to the Pak1 regulatory domain and prevents binding to the upstream activator Cdc42.
Targets
PAK1 [1]
(Cell-free assay)
2.5 μM
In vitro

IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1). PIR3.5 is the control compound of IPA-3. IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts. [1] IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NALM-6 cell NVXSZ5AzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF3qd2xKdmirYnn0bY9vKG:oIHj1cYFvKE6DTF2tOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQTd4OUSg{txO NUXxeGR[W0GQR1XS
human SW1710 cell M4nTWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVvLWJBUUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF5OEOzJO69VQ>? MWrTRW5ITVJ?
human YT cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\WUYNKdmirYnn0bY9vKG:oIHj1cYFvKFmWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6zPVA{PiEQvF2= NFPIeXdUSU6JRWK=
human BL-70 cell MnT1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlLZTY5pcWKrdHnvckBw\iCqdX3hckBDVC15MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNFM4QTZizszN NYjmVnZKW0GQR1XS
human SIG-M5 cell NHj1NnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4PFemlvcGmkaYTpc44hd2ZiaIXtZY4hW0mJLV21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4yODJzOTFOwG0> NF3sNWlUSU6JRWK=
human MCF7 cell Ml\uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXzJcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{LkG4NFch|ryP NUPwcYJpW0GQR1XS
human GB-1 cell M1PjWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYTkdWR{UW6qaXLpeIlwdiCxZjDoeY1idiCJQj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk43OzR7NDFOwG0> MlfnV2FPT0WU
human KU812 cell NUjQXGloT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUfJcohq[mm2aX;uJI9nKGi3bXHuJGtWQDF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj63OVk2KM7:TR?= NWnkXZY5W0GQR1XS
human QIMR-WIL cell NFTsVHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXPDSolYUW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwOUC4PVEh|ryP NVfuTVJlW0GQR1XS
human BL-41 cell MljZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGLVTndKdmirYnn0bY9vKG:oIHj1cYFvKEKOLUSxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4xQTh2NDFOwG0> M4nQb3NCVkeHUh?=
human CMK cell NG\TNphIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MonNTY5pcWKrdHnvckBw\iCqdX3hckBEVUtiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkG0OVk6KM7:TR?= MoPEV2FPT0WU
human U-698-M cell NWnVenZDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmHwTY5pcWKrdHnvckBw\iCqdX3hckBWNTZ7OD3NJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4zOzh3NjFOwG0> MXLTRW5ITVJ?
human Daoy cell NFL5VG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{TwT2lvcGmkaYTpc44hd2ZiaIXtZY4hTGGxeTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNlQ3PjJizszN MV\TRW5ITVJ?
human 786-0 cell M4LCTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MofsTY5pcWKrdHnvckBw\iCqdX3hckA4QDZvMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuN|E5ODVizszN NHW3S2NUSU6JRWK=
human REH cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmXyTY5pcWKrdHnvckBw\iCqdX3hckBTTUhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkO2OlU2KM7:TR?= MoL3V2FPT0WU
human MHH-PREB-1 cell NYi2dpF5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3vJXGlvcGmkaYTpc44hd2ZiaIXtZY4hVUiKLWDSSWIuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwNE[2PFch|ryP MYXTRW5ITVJ?
human KE-37 cell growth MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mny2TY5pcWKrdHnvckBw\iCqdX3hckBMTS1|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOVAzOzFizszN MnjLV2FPT0WU
human CAL-51 cell NIPtSXNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoTQTY5pcWKrdHnvckBw\iCqdX3hckBESUxvNUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlU1PTF7IN88US=> NU\kVppWW0GQR1XS
human CA46 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXLJcohq[mm2aX;uJI9nKGi3bXHuJGNCPDZiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lk[2NVgh|ryP M{j6NHNCVkeHUh?=
human HEL cell NXrTb5E6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWH5fpNGUW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlczPzZ6IN88US=> MXPTRW5ITVJ?
human NB6 cell NFrUfZZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXzmOIZvUW6qaXLpeIlwdiCxZjDoeY1idiCQQk[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlg{PzVzIN88US=> NY\ieG5xW0GQR1XS
human 8305C cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGGyNZNKdmirYnn0bY9vKG:oIHj1cYFvKDh|MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yPjd3ODFOwG0> M3X5eXNCVkeHUh?=
human EW-13 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIHjWItKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zODF2ODFOwG0> MY\TRW5ITVJ?
human NB13 cell Mle2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF65bWlKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlIzPTZizszN MXjTRW5ITVJ?
human EB2 cell M{LqTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYDJcohq[mm2aX;uJI9nKGi3bXHuJGVDOiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwNEmxOFIh|ryP M1nldHNCVkeHUh?=
human HOS cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV;ZOG43UW6qaXLpeIlwdiCxZjDoeY1idiCKT2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfS=> NFPKbFBUSU6JRWK=
human HLE cell MkmzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIjNS4NKdmirYnn0bY9vKG:oIHj1cYFvKEiORTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOVIyPDlizszN MXHTRW5ITVJ?
human ST486 cell M1q2PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn;STY5pcWKrdHnvckBw\iCqdX3hckBUXDR6NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuPFY2PCEQvF2= NVzWOWtyW0GQR1XS
human NCI-H2009 cell MkLJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF\uWZdKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlAxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwMESzOVQh|ryP NFTRdW9USU6JRWK=
human NH-12 cell MkTBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MojoTY5pcWKrdHnvckBw\iCqdX3hckBPUC1zMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNVE4PiEQvF2= NHvtW4ZUSU6JRWK=
human BFTC-905 cell NHuwSlRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkDVTY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOTR5Nkig{txO NVP5ZXZbW0GQR1XS
human RKO cell NFP4cVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3LmNmlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6yPFQ4OSEQvF2= NGjx[YdUSU6JRWK=
human PF-382 MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYTJcohq[mm2aX;uJI9nKGi3bXHuJHBHNTN6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|UzOjZizszN MoDRV2FPT0WU
human SR cell Mn\BS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3XpZ2lvcGmkaYTpc44hd2ZiaIXtZY4hW1JiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkO3NlU3KM7:TR?= NXPSSIoxW0GQR1XS
human WSU-NHL cell M3rmVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJHdUXS2QSFygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlUzODZ5IN88US=> MVvTRW5ITVJ?
human CAL-12T cell M1Pke2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NELONphKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2xNnQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjZ2MUSg{txO Mne3V2FPT0WU
human Daudi cell NHP2e4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFGyUopKdmirYnn0bY9vKG:oIHj1cYFvKESjdXTpJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QDR5NzFOwG0> Mnn3V2FPT0WU
human MC-CAR cell NHz6VVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17MXGlvcGmkaYTpc44hd2ZiaIXtZY4hVUNvQ1HSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44ODZ2NDFOwG0> MXHTRW5ITVJ?
human RPMI-8402 cell M2S5Rmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MknkTY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUi0NFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd4OEe2JO69VQ>? MlPNV2FPT0WU
human CCRF-CEM cell NGK1Z|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3HIcGlvcGmkaYTpc44hd2ZiaIXtZY4hS0OURj3DSW0h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njl5Nk[3JO69VQ>? MlfpV2FPT0WU
human CHP-126 cell M17O[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3vqO2lvcGmkaYTpc44hd2ZiaIXtZY4hS0iSLUGyOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvPzR{Nkig{txO NIrKPIFUSU6JRWK=
human NB69 cell NGnTToNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXHXTVc6UW6qaXLpeIlwdiCxZjDoeY1idiCQQk[5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok44PjN3MjFOwG0> M3XheHNCVkeHUh?=
human KS-1 cell NFPrR25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2XKO2lvcGmkaYTpc44hd2ZiaIXtZY4hU1NvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uPFg2PDlizszN MXXTRW5ITVJ?
human J82 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWTJcohq[mm2aX;uJI9nKGi3bXHuJGo5OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMES5OlUh|ryP MWfTRW5ITVJ?
human SW872 cell NWHuW5lYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXjJcohq[mm2aX;uJI9nKGi3bXHuJHNYQDd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz64N|U2QCEQvF2= M2r4bHNCVkeHUh?=
human HC-1 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGi3bXHuJGhENTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkC2PFA2KM7:TR?= M1W4bnNCVkeHUh?=
human DU-145 cell NY\hXFZOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkfUTY5pcWKrdHnvckBw\iCqdX3hckBFXS1zNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlIyPDZ2IN88US=> NILIWmhUSU6JRWK=
human HL-60 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXrBe2RyUW6qaXLpeIlwdiCxZjDoeY1idiCKTD22NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvQDR4MEWg{txO M4PON3NCVkeHUh?=
human MV-4-11 cell MkHyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkDHTY5pcWKrdHnvckBw\iCqdX3hckBOXi12LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU4yPjR7IN88US=> Mom4V2FPT0WU
human SK-N-DZ cell MmjIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWLpbZlNUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWRbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5{MESyN{DPxE1? M1r0WXNCVkeHUh?=
human HT-1197 cell Mn7iS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{fybGlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUG5O{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjNyN{Og{txO M{nHUnNCVkeHUh?=
human NOS-1 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXHIdWEyUW6qaXLpeIlwdiCxZjDoeY1idiCQT2OtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjdzNUKg{txO NHexNIlUSU6JRWK=
human HT-1080 cell NYLBWpZ5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUXJcohq[mm2aX;uJI9nKGi3bXHuJGhVNTFyOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE43Ojh|IN88US=> NXTlbFNMW0GQR1XS
human SK-LU-1 cell M3\tdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3SwPWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTGWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5Njh5M{Gg{txO MUDTRW5ITVJ?
human SW48 cell MnvsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnHkTY5pcWKrdHnvckBw\iCqdX3hckBUXzR6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkGuPFg6PyEQvF2= MWPTRW5ITVJ?
human A2780 cell NEXpSI1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVfJcohq[mm2aX;uJI9nKGi3bXHuJGEzPzhyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuN|cyKM7:TR?= NWLE[YtpW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 30971268     


The ESCC cells were exposed to increasing concentrations of IPA-3 for 72 h, the cell viability was measured by MTT assay. c-d KYSE30 and KYSE150 cells were exposed to increasing concentrations of IPA-3 for 48 h. 

30971268
Western blot
p-PAK1 / PAK1 / p-PAK2 / PAK2 / p-Raf1 / Raf1 / p-MEK1 / MEK1 / p-ERK / ERK ; 

PubMed: 30971268     


KYSE30 and KYSE150 cells were treated with indicated concentration of IPA-3 for 48 h, and then the protein levels of p-PAK1 (T423), PAK1, p-PAK2 (S141), PAK2, Raf1, p-Raf1 (S338), MEK1, p-MEK1 (S298), ERK1/2, p-ERK1/2 (T202/Y204) were detected by Western blotting analysis.

30971268
Immunofluorescence
BiP; 

PubMed: 24844382     


Cells were pretreated with 50 µM IPA-3 for 30 min and then incubated with Alexa555-labeled SubAB for 1 h at 37 °Cells were imaged by confocal microscopy for colocalization of SubAB (red) with BiP (green) as described in Materials and Methods. Bars represent 20 µm. Experiments were repeated two times with similar results.

NF-κB; 

PubMed: 23894351     


Effect of IPA-3 on subcellular localization of NF-κB was evaluated by immunofluorescence staining. After overnight serum starvation, H2M (left panel) and MIHA (right panel) cells were treated with either DMSO or IPA-3 (20 µM, 15 minutes) followed by an addition of TNF-α (20 ng/ml, 15 minutes). NF-κB was detected with a specific antibody (Green) and nucleus was stained with DAPI (Blue).

Paxillin; 

PubMed: 23894351     


Paxillin protein expression was detected by immunofluorescence analysis under IPA-3 treatment. H2M and H2P (upper panel) cells were serum-starved overnight and treated with either DMSO control or IPA-3 (20 µM) for 15 minutes, followed by FBS replenishment for 10 minutes. Immunofluoresence signals of phalloidin (Red), paxillin (Green) and DAPI (Blue) represent stress fiber, focal adhesion and nucleus, respectively (magnification 40X). The number of focal adhesion (paxillin) were counted in H2M (lower, left panel) and H2P (lower, right panel), and represented in the bar chart. Error bars, mean ± SD of triplicate samples. *P<0.01 (t-test) compared with DMSO control.

24844382 23894351

Protocol

Solubility (25°C)

In vitro DMSO 70 mg/mL (199.74 mM)
Ethanol 7 mg/mL (19.97 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 350.45
Formula

C20H14O2S2

CAS No. 42521-82-4
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C2C(=C1)C=CC(=C2SSC3=C(C=CC4=CC=CC=C43)O)O

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    If this product is cell membrane permeable and would it be suitable for PAK1 inhibition on a pancreatic beta cell line?

  • Answer:

    Based on the reference, I think IPA-3 is able to penetrate the cell membrane, it's likely to inhibit PAK1 on a pancreatic beta cell line although there is no reference confirming it: Figure 3: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963893/; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353635/

PAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID