IPA-3

For research use only.

Catalog No.S7093

32 publications

IPA-3 Chemical Structure

CAS No. 42521-82-4

IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).

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Selleck's IPA-3 has been cited by 32 publications

Purity & Quality Control

Choose Selective PAK Inhibitors

Biological Activity

Description IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).
Features IPA-3 binds covalently to the Pak1 regulatory domain and prevents binding to the upstream activator Cdc42.
Targets
PAK1 [1]
(Cell-free assay)
2.5 μM
In vitro

IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1). PIR3.5 is the control compound of IPA-3. IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts. [1] IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NALM-6 cell NYfrbWw3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUnQcI5CUW6qaXLpeIlwdiCxZjDoeY1idiCQQVzNMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjl5Nkm0JO69VQ>? NGH3XJBUSU6JRWK=
human SW1710 cell M1jaOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUXJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNVc5OzNizszN NXXCe4JHW0GQR1XS
human YT cell Ml\TS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2HHOGlvcGmkaYTpc44hd2ZiaIXtZY4hYVRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkO5NFM3KM7:TR?= M13sV3NCVkeHUh?=
human BL-70 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4rGOmlvcGmkaYTpc44hd2ZiaIXtZY4hSkxvN{CgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlA{Pzl4IN88US=> NVSxZlFzW0GQR1XS
human SIG-M5 cell NHrnZ5VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWnPO4JxUW6qaXLpeIlwdiCxZjDoeY1idiCVSVetUVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjFyMkG5JO69VQ>? Mmj0V2FPT0WU
human MCF7 cell M4HEc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIrWV2dKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlE5ODdizszN NGS3NHFUSU6JRWK=
human GB-1 cell NGniO5hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmmxTY5pcWKrdHnvckBw\iCqdX3hckBISi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj62N|Q6PCEQvF2= M1nPV3NCVkeHUh?=
human KU812 cell NFfLbnBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlK5TY5pcWKrdHnvckBw\iCqdX3hckBMXThzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuO|U6PSEQvF2= MXHTRW5ITVJ?
human QIMR-WIL cell MmfNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4D6VWlvcGmkaYTpc44hd2ZiaIXtZY4hWUmPUj3XTWwh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjlyOEmxJO69VQ>? M33lNnNCVkeHUh?=
human BL-41 cell NF\rbIxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Moq1TY5pcWKrdHnvckBw\iCqdX3hckBDVC12MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFk5PDRizszN M1zxfXNCVkeHUh?=
human CMK cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUjRbWxGUW6qaXLpeIlwdiCxZjDoeY1idiCFTVugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlE1PTl7IN88US=> NEL0U5ZUSU6JRWK=
human U-698-M cell NXvrcFlLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVHDc45{UW6qaXLpeIlwdiCxZjDoeY1idiCXLU[5PE1OKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{i1OkDPxE1? NUDzdJFZW0GQR1XS
human Daoy cell NX[yUIV{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3nOUGlvcGmkaYTpc44hd2ZiaIXtZY4hTGGxeTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNlQ3PjJizszN MoKwV2FPT0WU
human 786-0 cell NVzKPWpZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2PnTWlvcGmkaYTpc44hd2ZiaIXtZY4hPzh4LUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlMyQDB3IN88US=> MYXTRW5ITVJ?
human REH cell NHPGOHhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLsNWpnUW6qaXLpeIlwdiCxZjDoeY1idiCURVigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlM3PjV3IN88US=> M1TW[HNCVkeHUh?=
human MHH-PREB-1 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;6bm9CUW6qaXLpeIlwdiCxZjDoeY1idiCPSFitVHJGSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz60OlY5PyEQvF2= M4jDVXNCVkeHUh?=
human KE-37 cell growth NIm5U2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGtGNTN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz61NFI{OSEQvF2= NXfKO|ZLW0GQR1XS
human CAL-51 cell MnHuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3jRNGlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{42PDVzOTFOwG0> MmKyV2FPT0WU
human CA46 cell M2nnOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWTJcohq[mm2aX;uJI9nKGi3bXHuJGNCPDZiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lk[2NVgh|ryP NX3RfnB3W0GQR1XS
human HEL cell NUOySW1ET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NW\3eHp6UW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlczPzZ6IN88US=> Mkn1V2FPT0WU
human NB6 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJG5DPiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwOEO3OVEh|ryP M2X2eHNCVkeHUh?=
human 8305C cell NH;Jd2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFuwXYxKdmirYnn0bY9vKG:oIHj1cYFvKDh|MEXDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4yPjd3ODFOwG0> MXPTRW5ITVJ?
human EW-13 cell NI[4WW9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYOwW|RrUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvOjBzNEig{txO M{\FSHNCVkeHUh?=
human NB13 cell NHz3bI1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVXmZmpyUW6qaXLpeIlwdiCxZjDoeY1idiCQQkGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOjV4IN88US=> NIfaR5dUSU6JRWK=
human EB2 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NICwUWlKdmirYnn0bY9vKG:oIHj1cYFvKEWEMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFkyPDJizszN NHzUcmNUSU6JRWK=
human HOS cell M3v5W2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIHS[|BKdmirYnn0bY9vKG:oIHj1cYFvKEiRUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5 MU\TRW5ITVJ?
human HLE cell MkPhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1;jN2lvcGmkaYTpc44hd2ZiaIXtZY4hUEyHIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND61NlE1QSEQvF2= M4\RTHNCVkeHUh?=
human ST486 cell NIDKTW5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJHNVPDh4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND64OlU1KM7:TR?= MW\TRW5ITVJ?
human NCI-H2009 cell M1m3cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3vuOWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS5yNEO1OEDPxE1? Mnz1V2FPT0WU
human NH-12 cell NYG4NGg4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml7STY5pcWKrdHnvckBw\iCqdX3hckBPUC1zMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNVE4PiEQvF2= Mn:wV2FPT0WU
human BFTC-905 cell NEXuWFVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYLkOXc2UW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwMUS3Olgh|ryP MXXTRW5ITVJ?
human RKO cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M37yeGlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6yPFQ4OSEQvF2= NEnsWlVUSU6JRWK=
human PF-382 M4XxNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnLCTY5pcWKrdHnvckBw\iCqdX3hckBRTi1|OEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlM2OjJ4IN88US=> MYDTRW5ITVJ?
human SR cell M3HCN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIW1eXdKdmirYnn0bY9vKG:oIHj1cYFvKFOUIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6zO|I2PiEQvF2= NWPBdJd6W0GQR1XS
human WSU-NHL cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFj1RnVKdmirYnn0bY9vKG:oIHj1cYFvKFeVVT3OTGwh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjV{ME[3JO69VQ>? NIXa[HRUSU6JRWK=
human CAL-12T cell M2D4[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUW1UWc6UW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNVJVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS54NEG0JO69VQ>? NWm3dY96W0GQR1XS
human Daudi cell M3zUT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX;IW3R{UW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPjh2N{eg{txO MlqxV2FPT0WU
human MC-CAR cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\hTY5pcWKrdHnvckBw\iCqdX3hckBOSy2FQWKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlcxPjR2IN88US=> NXvVdllZW0GQR1XS
human RPMI-8402 cell NG\nUlBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVvvfZhzUW6qaXLpeIlwdiCxZjDoeY1idiCUUF3JMVg1ODJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lke2PFc3KM7:TR?= MonCV2FPT0WU
human CCRF-CEM cell M4\FSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUHJcohq[mm2aX;uJI9nKGi3bXHuJGNEWkZvQ1XNJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PzZ4NzFOwG0> MVvTRW5ITVJ?
human CHP-126 cell MoLzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mlm2TY5pcWKrdHnvckBw\iCqdX3hckBEUFBvMUK2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok44PDJ4ODFOwG0> NW\DS4IxW0GQR1XS
human NB69 cell Mnf6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlrsTY5pcWKrdHnvckBw\iCqdX3hckBPSjZ7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj63OlM2OiEQvF2= NV;JWFFxW0GQR1XS
human KS-1 cell NYTkbIxVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoKyTY5pcWKrdHnvckBw\iCqdX3hckBMWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj64PFU1QSEQvF2= M{\xU3NCVkeHUh?=
human J82 cell Mn:3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4KxTmlvcGmkaYTpc44hd2ZiaIXtZY4hUjh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nz6wOFk3PSEQvF2= NEW2TWhUSU6JRWK=
human SW872 cell NG\hclZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn74TY5pcWKrdHnvckBw\iCqdX3hckBUXzh5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuPFM2PThizszN MoftV2FPT0WU
human HC-1 cell NVjYcopIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWTQeWRIUW6qaXLpeIlwdiCxZjDoeY1idiCKQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE4xPjhyNTFOwG0> M1PIPHNCVkeHUh?=
human DU-145 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlE1PjRizszN MnrQV2FPT0WU
human HL-60 cell NFH0TnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH\3UZJKdmirYnn0bY9vKG:oIHj1cYFvKEiOLU[wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE45PDZyNTFOwG0> MWjTRW5ITVJ?
human MV-4-11 cell NHm1eZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGTWXVhKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjF4NEmg{txO M{\RdXNCVkeHUh?=
human SK-N-DZ cell NGnrTHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4rpS2lvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTj3EXkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOjB2MkOg{txO MXrTRW5ITVJ?
human HT-1197 cell MoHiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXLGOIp5UW6qaXLpeIlwdiCxZjDoeY1idiCKVD2xNVk4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwM{C3N{DPxE1? NHPDO49USU6JRWK=
human NOS-1 cell NFnMUG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MY\Jcohq[mm2aX;uJI9nKGi3bXHuJG5QWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuO|E2OiEQvF2= M3\KR3NCVkeHUh?=
human HT-1080 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4nOeWlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUC4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5NjZ{OEOg{txO MYfTRW5ITVJ?
human SK-LU-1 cell MkjxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUX2dml5UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3MWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OThwOEezNUDPxE1? NF7iU3dUSU6JRWK=
human SW48 cell MoTCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHWyT3lKdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNU45QDl5IN88US=> MWXTRW5ITVJ?
human A2780 cell NVLGUXRKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFzmOWlKdmirYnn0bY9vKG:oIHj1cYFvKEF{N{iwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlgvOzdzIN88US=> M1rsN3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-PAK1 / PAK1 / p-PAK2 / PAK2 / p-Raf1 / Raf1 / p-MEK1 / MEK1 / p-ERK / ERK ; 

PubMed: 30971268     


KYSE30 and KYSE150 cells were treated with indicated concentration of IPA-3 for 48 h, and then the protein levels of p-PAK1 (T423), PAK1, p-PAK2 (S141), PAK2, Raf1, p-Raf1 (S338), MEK1, p-MEK1 (S298), ERK1/2, p-ERK1/2 (T202/Y204) were detected by Western blotting analysis.

30971268
Growth inhibition assay
Cell viability; 

PubMed: 30971268     


The ESCC cells were exposed to increasing concentrations of IPA-3 for 72 h, the cell viability was measured by MTT assay. c-d KYSE30 and KYSE150 cells were exposed to increasing concentrations of IPA-3 for 48 h. 

30971268
Immunofluorescence
Paxillin; 

PubMed: 23894351     


Paxillin protein expression was detected by immunofluorescence analysis under IPA-3 treatment. H2M and H2P (upper panel) cells were serum-starved overnight and treated with either DMSO control or IPA-3 (20 µM) for 15 minutes, followed by FBS replenishment for 10 minutes. Immunofluoresence signals of phalloidin (Red), paxillin (Green) and DAPI (Blue) represent stress fiber, focal adhesion and nucleus, respectively (magnification 40X). The number of focal adhesion (paxillin) were counted in H2M (lower, left panel) and H2P (lower, right panel), and represented in the bar chart. Error bars, mean ± SD of triplicate samples. *P<0.01 (t-test) compared with DMSO control.

NF-κB; 

PubMed: 23894351     


Effect of IPA-3 on subcellular localization of NF-κB was evaluated by immunofluorescence staining. After overnight serum starvation, H2M (left panel) and MIHA (right panel) cells were treated with either DMSO or IPA-3 (20 µM, 15 minutes) followed by an addition of TNF-α (20 ng/ml, 15 minutes). NF-κB was detected with a specific antibody (Green) and nucleus was stained with DAPI (Blue).

BiP; 

PubMed: 24844382     


Cells were pretreated with 50 µM IPA-3 for 30 min and then incubated with Alexa555-labeled SubAB for 1 h at 37 °Cells were imaged by confocal microscopy for colocalization of SubAB (red) with BiP (green) as described in Materials and Methods. Bars represent 20 µm. Experiments were repeated two times with similar results.

23894351 24844382

Protocol

Solubility (25°C)

In vitro DMSO 70 mg/mL (199.74 mM)
Ethanol 7 mg/mL (19.97 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 350.45
Formula

C20H14O2S2

CAS No. 42521-82-4
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C2C(=C1)C=CC(=C2SSC3=C(C=CC4=CC=CC=C43)O)O

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    If this product is cell membrane permeable and would it be suitable for PAK1 inhibition on a pancreatic beta cell line?

  • Answer:

    Based on the reference, I think IPA-3 is able to penetrate the cell membrane, it's likely to inhibit PAK1 on a pancreatic beta cell line although there is no reference confirming it: Figure 3: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963893/; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353635/

PAK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID