IPA-3

For research use only.

Catalog No.S7093

37 publications

IPA-3 Chemical Structure

CAS No. 42521-82-4

IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).

Selleck's IPA-3 has been cited by 37 publications

Purity & Quality Control

Choose Selective PAK Inhibitors

Biological Activity

Description IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).
Features IPA-3 binds covalently to the Pak1 regulatory domain and prevents binding to the upstream activator Cdc42.
Targets
PAK1 [1]
(Cell-free assay)
2.5 μM
In vitro

IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1). PIR3.5 is the control compound of IPA-3. IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts. [1] IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NALM-6 cell Ml;kS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYS1WYJ{UW6qaXLpeIlwdiCxZjDoeY1idiCQQVzNMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjl5Nkm0JO69VQ>? Mmm1V2FPT0WU
human SW1710 cell MmixS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoTGTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlE4QDN|IN88US=> NWLaNlFjW0GQR1XS
human YT cell M2DIRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mm[xTY5pcWKrdHnvckBw\iCqdX3hckB[XCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwM{mwN|Yh|ryP NVXmU2RRW0GQR1XS
human BL-70 cell NF2yb2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGJNNTdyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6wN|c6PiEQvF2= NVi5N2J7W0GQR1XS
human SIG-M5 cell NGjZRYNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJHNKTy2PNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNVAzOTlizszN M{PaR3NCVkeHUh?=
human MCF7 cell MoHRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1jyRWlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNVgxPyEQvF2= Mn;MV2FPT0WU
human GB-1 cell Mo\uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NULrTI5UUW6qaXLpeIlwdiCxZjDoeY1idiCJQj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk43OzR7NDFOwG0> NF\6[npUSU6JRWK=
human KU812 cell NIrtNnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{jHV2lvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlc2QTVizszN NIjGdVZUSU6JRWK=
human QIMR-WIL cell M3Hy[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUjJcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk46ODh7MTFOwG0> MVTTRW5ITVJ?
human BL-41 cell NUT5bpNVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlrOTY5pcWKrdHnvckBw\iCqdX3hckBDVC12MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNFk5PDRizszN NF3iUWRUSU6JRWK=
human CMK cell NXTmUoFST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH7DSXFKdmirYnn0bY9vKG:oIHj1cYFvKEOPSzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNVQ2QTlizszN Mmr2V2FPT0WU
human U-698-M cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYq0XZZKUW6qaXLpeIlwdiCxZjDoeY1idiCXLU[5PE1OKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{i1OkDPxE1? Ml\IV2FPT0WU
human Daoy cell NFzt[VJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWPJcohq[mm2aX;uJI9nKGi3bXHuJGRid3liY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkK0OlYzKM7:TR?= M3WwPXNCVkeHUh?=
human 786-0 cell NXrMbJNzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4TiN2lvcGmkaYTpc44hd2ZiaIXtZY4hPzh4LUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlMyQDB3IN88US=> MojnV2FPT0WU
human REH cell NX7jUIZGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHHNbo5KdmirYnn0bY9vKG:oIHj1cYFvKFKHSDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuN|Y3PTVizszN MUXTRW5ITVJ?
human MHH-PREB-1 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH;teHRKdmirYnn0bY9vKG:oIHj1cYFvKE2KSD3QVmVDNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkS2Olg4KM7:TR?= NIGyepJUSU6JRWK=
human KE-37 cell growth NFflbGVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{j4c2lvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlUxOjNzIN88US=> MkG3V2FPT0WU
human CAL-51 cell Ml3ZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYPJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOVQ2OTlizszN NWrJbVVRW0GQR1XS
human CA46 cell NY\6XHJWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXHQfpk6UW6qaXLpeIlwdiCxZjDoeY1idiCFQUS2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{43PjF6IN88US=> M2jHV3NCVkeHUh?=
human HEL cell NIjTTplIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUnJcohq[mm2aX;uJI9nKGi3bXHuJGhGVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwN{K3Olgh|ryP NX34PIM3W0GQR1XS
human NB6 cell M13MTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFW2XWVKdmirYnn0bY9vKG:oIHj1cYFvKE6ENjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuPFM4PTFizszN NHW0XpZUSU6JRWK=
human 8305C cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{nCUGlvcGmkaYTpc44hd2ZiaIXtZY4hQDNyNVOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlE3PzV6IN88US=> M{nad3NCVkeHUh?=
human EW-13 cell M2PCRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGTrdGdKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zODF2ODFOwG0> NYjhWXMyW0GQR1XS
human NB13 cell MkK1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4TGPWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuNlI2PiEQvF2= NWXDbFhvW0GQR1XS
human EB2 cell M33kbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn3rTY5pcWKrdHnvckBw\iCqdX3hckBGSjJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12LkS5NVQzKM7:TR?= MUfTRW5ITVJ?
human HOS cell NV;rXHZNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M322NGlvcGmkaYTpc44hd2ZiaIXtZY4hUE:VIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYm= MnryV2FPT0WU
human HLE cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUnJNINXUW6qaXLpeIlwdiCxZjDoeY1idiCKTFWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlUzOTR7IN88US=> NF;qVYpUSU6JRWK=
human ST486 cell NI\JN4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEOxUXdKdmirYnn0bY9vKG:oIHj1cYFvKFOWNEi2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE45PjV2IN88US=> NF;MWJhUSU6JRWK=
human NCI-H2009 cell NYjGOWR3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmWxTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjB2M{W0JO69VQ>? NVriN3dqW0GQR1XS
human NH-12 cell NIrIWodIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYHJcohq[mm2aX;uJI9nKGi3bXHuJG5JNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6xNVc3KM7:TR?= NULL[4w2W0GQR1XS
human BFTC-905 cell NXTCb|gzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mo\0TY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOTR5Nkig{txO NGGy[phUSU6JRWK=
human RKO cell NF\iSGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17yWmlvcGmkaYTpc44hd2ZiaIXtZY4hWkuRIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT6yPFQ4OSEQvF2= NV3UVIRYW0GQR1XS
human PF-382 MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVHnUVE2UW6qaXLpeIlwdiCxZjDoeY1idiCSRj2zPFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjN3MkK2JO69VQ>? MUjTRW5ITVJ?
human SR cell NWqyRnFzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV7DUJhFUW6qaXLpeIlwdiCxZjDoeY1idiCVUjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuN|czPTZizszN MnjpV2FPT0WU
human WSU-NHL cell M{nzcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MojKTY5pcWKrdHnvckBw\iCqdX3hckBYW1VvTljMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU42OjB4NzFOwG0> NHXEfmtUSU6JRWK=
human CAL-12T cell M164cmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF;5c3lKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2xNnQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjZ2MUSg{txO NXjzdFgzW0GQR1XS
human Daudi cell Mn\0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXzZRXJzUW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPjh2N{eg{txO M4HVfXNCVkeHUh?=
human MC-CAR cell M{\IWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4DHVmlvcGmkaYTpc44hd2ZiaIXtZY4hVUNvQ1HSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44ODZ2NDFOwG0> MVzTRW5ITVJ?
human RPMI-8402 cell Ml[3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXTJcohq[mm2aX;uJI9nKGi3bXHuJHJRVUlvOESwNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzZ6N{[g{txO NFX3XZpUSU6JRWK=
human CCRF-CEM cell MlHIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGNEWkZvQ1XNJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU46PzZ4NzFOwG0> MmDkV2FPT0WU
human CHP-126 cell M2THcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF:4T3ZKdmirYnn0bY9vKG:oIHj1cYFvKEOKUD2xNlYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03Njd2Mk[4JO69VQ>? NGG2cWVUSU6JRWK=
human NB69 cell MnL6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFXXfJNKdmirYnn0bY9vKG:oIHj1cYFvKE6ENkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22Mlc3OzV{IN88US=> NFrDSHBUSU6JRWK=
human KS-1 cell M4fRUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkL2TY5pcWKrdHnvckBw\iCqdX3hckBMWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj64PFU1QSEQvF2= MY\TRW5ITVJ?
human J82 cell NWD4OIN6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVfvVWY2UW6qaXLpeIlwdiCxZjDoeY1idiCMOEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlA1QTZ3IN88US=> MYDTRW5ITVJ?
human SW872 cell M2ewS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NW\5VJpyUW6qaXLpeIlwdiCxZjDoeY1idiCVV{i3NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvQDN3NUig{txO NFHRNGFUSU6JRWK=
human HC-1 cell NHfIN3VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2HGVWlvcGmkaYTpc44hd2ZiaIXtZY4hUENvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNFY5ODVizszN NV3ycnhqW0GQR1XS
human DU-145 cell Mn3aS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkDiTY5pcWKrdHnvckBw\iCqdX3hckBFXS1zNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlIyPDZ2IN88US=> MV;TRW5ITVJ?
human HL-60 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmLxTY5pcWKrdHnvckBw\iCqdX3hckBJVC14MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuPFQ3ODVizszN M{XKZXNCVkeHUh?=
human MV-4-11 cell NEHwNJJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXfJcohq[mm2aX;uJI9nKGi3bXHuJG1XNTRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlE3PDlizszN NWnHeW9KW0GQR1XS
human SK-N-DZ cell MnTpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYLwOpd{UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3OMWRbKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5{MESyN{DPxE1? NXXCZVBuW0GQR1XS
human HT-1197 cell MmHIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2f6UmlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUG5O{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjNyN{Og{txO MlzPV2FPT0WU
human NOS-1 cell M{G4b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXHJcohq[mm2aX;uJI9nKGi3bXHuJG5QWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuO|E2OiEQvF2= MlnYV2FPT0WU
human HT-1080 cell NITNNWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFPZ[YJKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUGwPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQC54MkizJO69VQ>? MmDwV2FPT0WU
human SK-LU-1 cell MnzKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFW2T|VKdmirYnn0bY9vKG:oIHj1cYFvKFONLVzVMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQC56N{OxJO69VQ>? MmDNV2FPT0WU
human SW48 cell NIjGPFlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWrJcohq[mm2aX;uJI9nKGi3bXHuJHNYPDhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{MT64PFk4KM7:TR?= NYPlT5VXW0GQR1XS
human A2780 cell M4T0cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGEzPzhyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuN|cyKM7:TR?= NVLvV|VEW0GQR1XS

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 30971268     


The ESCC cells were exposed to increasing concentrations of IPA-3 for 72 h, the cell viability was measured by MTT assay. c-d KYSE30 and KYSE150 cells were exposed to increasing concentrations of IPA-3 for 48 h. 

30971268
Western blot
p-PAK1 / PAK1 / p-PAK2 / PAK2 / p-Raf1 / Raf1 / p-MEK1 / MEK1 / p-ERK / ERK ; 

PubMed: 30971268     


KYSE30 and KYSE150 cells were treated with indicated concentration of IPA-3 for 48 h, and then the protein levels of p-PAK1 (T423), PAK1, p-PAK2 (S141), PAK2, Raf1, p-Raf1 (S338), MEK1, p-MEK1 (S298), ERK1/2, p-ERK1/2 (T202/Y204) were detected by Western blotting analysis.

30971268
Immunofluorescence
BiP; 

PubMed: 24844382     


Cells were pretreated with 50 µM IPA-3 for 30 min and then incubated with Alexa555-labeled SubAB for 1 h at 37 °Cells were imaged by confocal microscopy for colocalization of SubAB (red) with BiP (green) as described in Materials and Methods. Bars represent 20 µm. Experiments were repeated two times with similar results.

NF-κB; 

PubMed: 23894351     


Effect of IPA-3 on subcellular localization of NF-κB was evaluated by immunofluorescence staining. After overnight serum starvation, H2M (left panel) and MIHA (right panel) cells were treated with either DMSO or IPA-3 (20 µM, 15 minutes) followed by an addition of TNF-α (20 ng/ml, 15 minutes). NF-κB was detected with a specific antibody (Green) and nucleus was stained with DAPI (Blue).

Paxillin; 

PubMed: 23894351     


Paxillin protein expression was detected by immunofluorescence analysis under IPA-3 treatment. H2M and H2P (upper panel) cells were serum-starved overnight and treated with either DMSO control or IPA-3 (20 µM) for 15 minutes, followed by FBS replenishment for 10 minutes. Immunofluoresence signals of phalloidin (Red), paxillin (Green) and DAPI (Blue) represent stress fiber, focal adhesion and nucleus, respectively (magnification 40X). The number of focal adhesion (paxillin) were counted in H2M (lower, left panel) and H2P (lower, right panel), and represented in the bar chart. Error bars, mean ± SD of triplicate samples. *P<0.01 (t-test) compared with DMSO control.

24844382 23894351

Protocol

Solubility (25°C)

In vitro DMSO 70 mg/mL (199.74 mM)
Ethanol 7 mg/mL (19.97 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 350.45
Formula

C20H14O2S2
CAS No. 42521-82-4
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C2C(=C1)C=CC(=C2SSC3=C(C=CC4=CC=CC=C43)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    If this product is cell membrane permeable and would it be suitable for PAK1 inhibition on a pancreatic beta cell line?

  • Answer:

    Based on the reference, I think IPA-3 is able to penetrate the cell membrane, it's likely to inhibit PAK1 on a pancreatic beta cell line although there is no reference confirming it: Figure 3: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963893/; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353635/

selleck catalog

PAK Signaling Pathway Map
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID