IPA-3

For research use only.

Catalog No.S7093

29 publications

IPA-3 Chemical Structure

CAS No. 42521-82-4

IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).

Size Price Stock Quantity  
USD 70 In stock
USD 370 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's IPA-3 has been cited by 29 publications

Purity & Quality Control

Choose Selective PAK Inhibitors

Biological Activity

Description IPA-3 is a selective non-ATP competitive Pak1 inhibitor with IC50 of 2.5 μM in a cell-free assay, no inhibition to group II PAKs (PAKs 4-6).
Features IPA-3 binds covalently to the Pak1 regulatory domain and prevents binding to the upstream activator Cdc42.
Targets
PAK1 [1]
(Cell-free assay)
2.5 μM
In vitro

IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1). PIR3.5 is the control compound of IPA-3. IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts. [1] IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human NALM-6 cell NIH0ToFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFfJTZFKdmirYnn0bY9vKG:oIHj1cYFvKE6DTF2tOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQTd4OUSg{txO M2m4S3NCVkeHUh?=
human SW1710 cell NXv3VI86T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWT6SVZ2UW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF5OEOzJO69VQ>? M2PUcXNCVkeHUh?=
human YT cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;QRYRzUW6qaXLpeIlwdiCxZjDoeY1idiC\VDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|kxOzZizszN NFnJXZNUSU6JRWK=
human BL-70 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGi3bXHuJGJNNTdyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj6wN|c6PiEQvF2= NVzINmp5W0GQR1XS
human SIG-M5 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3r5VWlvcGmkaYTpc44hd2ZiaIXtZY4hW0mJLV21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4yODJzOTFOwG0> NYfxUo4zW0GQR1XS
human MCF7 cell M1HTWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX[wc3B4UW6qaXLpeIlwdiCxZjDoeY1idiCPQ1[3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4yQDB5IN88US=> MVXTRW5ITVJ?
human GB-1 cell M4DmT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfSXmVxUW6qaXLpeIlwdiCxZjDoeY1idiCJQj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk43OzR7NDFOwG0> NIP5Zm1USU6JRWK=
human KU812 cell M3jX[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJGtWQDF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj63OVk2KM7:TR?= MV;TRW5ITVJ?
human QIMR-WIL cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWGxPXR5UW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwOUC4PVEh|ryP MnjlV2FPT0WU
human BL-41 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVm3ZVM{UW6qaXLpeIlwdiCxZjDoeY1idiCETD20NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvODl6NESg{txO NEPYNHJUSU6JRWK=
human CMK cell M2X0eGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVzJcohq[mm2aX;uJI9nKGi3bXHuJGNOUyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwMUS1PVkh|ryP NWK2O|NUW0GQR1XS
human U-698-M cell MnzwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV76TGNIUW6qaXLpeIlwdiCxZjDoeY1idiCXLU[5PE1OKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oy5{M{i1OkDPxE1? MorPV2FPT0WU
human Daoy cell M2jxOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{jNcmlvcGmkaYTpc44hd2ZiaIXtZY4hTGGxeTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuNlQ3PjJizszN NEjTemxUSU6JRWK=
human 786-0 cell M4jxemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mnz6TY5pcWKrdHnvckBw\iCqdX3hckA4QDZvMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuN|E5ODVizszN NF75VndUSU6JRWK=
human REH cell M2XJNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVTJcohq[mm2aX;uJI9nKGi3bXHuJHJGUCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwM{[2OVUh|ryP NH;EblBUSU6JRWK=
human MHH-PREB-1 cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYHJSll5UW6qaXLpeIlwdiCxZjDoeY1idiCPSFitVHJGSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz60OlY5PyEQvF2= M2rrSHNCVkeHUh?=
human KE-37 cell growth MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGtGNTN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz61NFI{OSEQvF2= MonFV2FPT0WU
human CAL-51 cell MlfGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYDJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuOVQ2OTlizszN M3LCb3NCVkeHUh?=
human CA46 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWTJcohq[mm2aX;uJI9nKGi3bXHuJGNCPDZiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lk[2NVgh|ryP Ml31V2FPT0WU
human HEL cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MliwTY5pcWKrdHnvckBw\iCqdX3hckBJTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkeyO|Y5KM7:TR?= M{XR[XNCVkeHUh?=
human NB6 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJG5DPiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwOEO3OVEh|ryP MVfTRW5ITVJ?
human 8305C cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVHPVXZ2UW6qaXLpeIlwdiCxZjDoeY1idiB6M{C1R{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvOTZ5NUig{txO MlPIV2FPT0WU
human EW-13 cell NVXo[pN6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVzJcohq[mm2aX;uJI9nKGi3bXHuJGVYNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND6yNFE1QCEQvF2= M4fWNXNCVkeHUh?=
human NB13 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYL5R5FUUW6qaXLpeIlwdiCxZjDoeY1idiCQQkGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4zOjV4IN88US=> M3\UPHNCVkeHUh?=
human EB2 cell M2\NOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGLPeXdKdmirYnn0bY9vKG:oIHj1cYFvKEWEMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOFkyPDJizszN MYXTRW5ITVJ?
human HOS cell Mk\CS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUnnV2N7UW6qaXLpeIlwdiCxZjDoeY1idiCKT2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfS=> MmHsV2FPT0WU
human HLE cell MoHDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIfIcG5KdmirYnn0bY9vKG:oIHj1cYFvKEiORTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSuOVIyPDlizszN M2PaNnNCVkeHUh?=
human ST486 cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUHJcohq[mm2aX;uJI9nKGi3bXHuJHNVPDh4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND64OlU1KM7:TR?= NH\tS2tUSU6JRWK=
human NCI-H2009 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4PSVmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PS5yNEO1OEDPxE1? NXrmcJJtW0GQR1XS
human NH-12 cell MofYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXzUeYtUUW6qaXLpeIlwdiCxZjDoeY1idiCQSD2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOTF5NjFOwG0> M3z3dnNCVkeHUh?=
human BFTC-905 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1PQb2lvcGmkaYTpc44hd2ZiaIXtZY4hSk[WQz25NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjF2N{[4JO69VQ>? NYmxPXM1W0GQR1XS
human RKO cell M1X1XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEnTVGpKdmirYnn0bY9vKG:oIHj1cYFvKFKNTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNlg1PzFizszN MUTTRW5ITVJ?
human PF-382 Mmf1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1;ROWlvcGmkaYTpc44hd2ZiaIXtZY4hWEZvM{iyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4{PTJ{NjFOwG0> MVPTRW5ITVJ?
human SR cell M2TVeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{TiVGlvcGmkaYTpc44hd2ZiaIXtZY4hW1JiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkO3NlU3KM7:TR?= NGG0TpVUSU6JRWK=
human WSU-NHL cell NYPmZWZkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXLJcohq[mm2aX;uJI9nKGi3bXHuJHdUXS2QSFygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlUzODZ5IN88US=> M1\3cnNCVkeHUh?=
human CAL-12T cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFj1TVRKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2xNnQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjZ2MUSg{txO NImzeZNUSU6JRWK=
human Daudi cell NH\HNGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFTV[pJKdmirYnn0bY9vKG:oIHj1cYFvKESjdXTpJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU43QDR5NzFOwG0> M2G1[3NCVkeHUh?=
human MC-CAR cell M4PuSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWn5RnRmUW6qaXLpeIlwdiCxZjDoeY1idiCPQz3DRXIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjdyNkS0JO69VQ>? M{D0V3NCVkeHUh?=
human RPMI-8402 cell NFG1cJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\rTY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUi0NFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd4OEe2JO69VQ>? NYPvZZBCW0GQR1XS
human CCRF-CEM cell NYDMWVdIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWi1bXoyUW6qaXLpeIlwdiCxZjDoeY1idiCFQ2LGMWNGVSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwOUe2Olch|ryP MXrTRW5ITVJ?
human CHP-126 cell NGTMO2xIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWnFVY92UW6qaXLpeIlwdiCxZjDoeY1idiCFSGCtNVI3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi55NEK2PEDPxE1? NFHwXW5USU6JRWK=
human NB69 cell NWi2NFJlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2juOWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uO|Y{PTJizszN M4O0bXNCVkeHUh?=
human KS-1 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mnu1TY5pcWKrdHnvckBw\iCqdX3hckBMWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj64PFU1QSEQvF2= MX7TRW5ITVJ?
human J82 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYLJcohq[mm2aX;uJI9nKGi3bXHuJGo5OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMES5OlUh|ryP NWixemQzW0GQR1XS
human SW872 cell NYqxdmVHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1LIVmlvcGmkaYTpc44hd2ZiaIXtZY4hW1d6N{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23Mlg{PTV6IN88US=> NHPGO4hUSU6JRWK=
human HC-1 cell NFu2VI1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NU\RXlM1UW6qaXLpeIlwdiCxZjDoeY1idiCKQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE4xPjhyNTFOwG0> Mn;vV2FPT0WU
human DU-145 cell NVzBSHJuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlE1PjRizszN MV7TRW5ITVJ?
human HL-60 cell NF;ISYVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2PPUmlvcGmkaYTpc44hd2ZiaIXtZY4hUExvNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24Mlg1PjB3IN88US=> NWK1bXNqW0GQR1XS
human MV-4-11 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{T6V2lvcGmkaYTpc44hd2ZiaIXtZY4hVVZvND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOTZ2OTFOwG0> NYnuVXpXW0GQR1XS
human SK-N-DZ cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU5vRGqgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlIxPDJ|IN88US=> M1\Dd3NCVkeHUh?=
human HT-1197 cell NHLiUFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1;KO2lvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUG5O{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjNyN{Og{txO NVPhfXpVW0GQR1XS
human NOS-1 cell M1fRTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkjGTY5pcWKrdHnvckBw\iCqdX3hckBPV1NvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlcyPTJizszN NFnmTVlUSU6JRWK=
human HT-1080 cell Mnf2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGT0b|NKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUGwPFAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQC54MkizJO69VQ>? MVjTRW5ITVJ?
human SK-LU-1 cell M2qxcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1XWUmlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTGWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5Njh5M{Gg{txO NWPveoluW0GQR1XS
human SW48 cell NWXaXldvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIfSPI1KdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNU45QDl5IN88US=> NF7jSlRUSU6JRWK=
human A2780 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17QOWlvcGmkaYTpc44hd2ZiaIXtZY4hSTJ5OECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE4{PzFizszN NHHEVWtUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-PAK1 / PAK1 / p-PAK2 / PAK2 / p-Raf1 / Raf1 / p-MEK1 / MEK1 / p-ERK / ERK ; 

PubMed: 30971268     


KYSE30 and KYSE150 cells were treated with indicated concentration of IPA-3 for 48 h, and then the protein levels of p-PAK1 (T423), PAK1, p-PAK2 (S141), PAK2, Raf1, p-Raf1 (S338), MEK1, p-MEK1 (S298), ERK1/2, p-ERK1/2 (T202/Y204) were detected by Western blotting analysis.

30971268
Growth inhibition assay
Cell viability; 

PubMed: 30971268     


The ESCC cells were exposed to increasing concentrations of IPA-3 for 72 h, the cell viability was measured by MTT assay. c-d KYSE30 and KYSE150 cells were exposed to increasing concentrations of IPA-3 for 48 h. 

30971268
Immunofluorescence
Paxillin; 

PubMed: 23894351     


Paxillin protein expression was detected by immunofluorescence analysis under IPA-3 treatment. H2M and H2P (upper panel) cells were serum-starved overnight and treated with either DMSO control or IPA-3 (20 µM) for 15 minutes, followed by FBS replenishment for 10 minutes. Immunofluoresence signals of phalloidin (Red), paxillin (Green) and DAPI (Blue) represent stress fiber, focal adhesion and nucleus, respectively (magnification 40X). The number of focal adhesion (paxillin) were counted in H2M (lower, left panel) and H2P (lower, right panel), and represented in the bar chart. Error bars, mean ± SD of triplicate samples. *P<0.01 (t-test) compared with DMSO control.

NF-κB; 

PubMed: 23894351     


Effect of IPA-3 on subcellular localization of NF-κB was evaluated by immunofluorescence staining. After overnight serum starvation, H2M (left panel) and MIHA (right panel) cells were treated with either DMSO or IPA-3 (20 µM, 15 minutes) followed by an addition of TNF-α (20 ng/ml, 15 minutes). NF-κB was detected with a specific antibody (Green) and nucleus was stained with DAPI (Blue).

BiP; 

PubMed: 24844382     


Cells were pretreated with 50 µM IPA-3 for 30 min and then incubated with Alexa555-labeled SubAB for 1 h at 37 °Cells were imaged by confocal microscopy for colocalization of SubAB (red) with BiP (green) as described in Materials and Methods. Bars represent 20 µm. Experiments were repeated two times with similar results.

23894351 24844382

Protocol

Solubility (25°C)

In vitro DMSO 70 mg/mL (199.74 mM)
Ethanol 7 mg/mL (19.97 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 350.45
Formula

C20H14O2S2

CAS No. 42521-82-4
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C2C(=C1)C=CC(=C2SSC3=C(C=CC4=CC=CC=C43)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    If this product is cell membrane permeable and would it be suitable for PAK1 inhibition on a pancreatic beta cell line?

  • Answer:

    Based on the reference, I think IPA-3 is able to penetrate the cell membrane, it's likely to inhibit PAK1 on a pancreatic beta cell line although there is no reference confirming it: Figure 3: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963893/; http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353635/

PAK Signaling Pathway Map

Tags: buy IPA-3 | IPA-3 supplier | purchase IPA-3 | IPA-3 cost | IPA-3 manufacturer | order IPA-3 | IPA-3 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID