Name: Lomustine
Brand: Selleck Chemicals
SKU: S1840
Availability: InStock
Rating: 5 (23 reviews)

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Quality Control

Batch: Purity: 99.89%

99.89

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN Topoisomerase PPAR Sirtuin Casein Kinase eIF
Other DNA/RNA Synthesis Inhibitors	CX-5461 (Pidnarulex) SCR7 Favipiravir (T-705) RK-33 EED226 BMH-21 Carmofur Triapine (3-AP) YK-4-279 Halofuginone

Solubility

In vitro
Batch:

DMSO : 47 mg/mL (201.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	30%propylene glycol 1 5%Tween80 2 65%D5W, pH 4 Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (42.79mM)	Taking the 1 mL working solution as an example, add 300 μL of clarified propylene glycol stock solution of 33.33 mg/ml to 50 μL of Tween 80, mix evenly to clarify it; then continue to add 650 μL of D5W, pH 4, to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	233.7	Formula	C₉H₁₆ClN₃O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	13010-47-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC79037, Gleostine, CeeNU, CCNU			SMILES	C1CCC(CC1)NC(=O)N(CCCl)N=O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Features	A more specific and potent anti-medulloblastoma agent compared to Vincristine.
In vitro	Lomustine inhibits the growth of ZR-75-1 and U373 with IC50 of 12 μM and 15 μM, respectively. This compound reduces the level of expression of the DNA repair protein O6-alkylguanine-DNA alkyltransferase. It (420 μM) triggers apoptosis through the mitochondrial pathway via decrease in the level of the anti-apoptosis proteins Bcl-2 and Bcl-xl, respectively, in both medulloblastoma and normal human epithelial and fibroblast cells. This chemical induces cell cycle delay in G2/M phase in medulloblastoma cells and up-regulates p21 protein level in a p53-independent manner in HFSN1 cells.
In vivo	Lomustine can cause delayed, cumulative dose-related, chronic hepatotoxicity that is irreversible and can be fatal. This compound could result in infrequent severe hematological toxicity in cats with spontaneously arising tumors, and the incidence of either grade III or IV neutropenia and thrombocytopenia is 4.1% and 1.0%, respectively. It trends toward a greater likelihood for progressive neutropenia and statistically significant higher response rates in cats with spontaneously arising tumors.
References	[1] https://pubmed.ncbi.nlm.nih.gov/8512814/ [2] https://pubmed.ncbi.nlm.nih.gov/18058069/ [3] https://pubmed.ncbi.nlm.nih.gov/10587263/ [4] https://pubmed.ncbi.nlm.nih.gov/14765735/ [5] https://pubmed.ncbi.nlm.nih.gov/12118689/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-04-13)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06419946	RECRUITING	Glioblastoma, IDH-wildtype; MGMT-Methylated Glioblastoma	Vastra Gotaland Region	2025-02-01	PHASE3
NCT07629089	NOT_YET_RECRUITING	Brain Cancer	Megan Mantica	2026-06-30	PHASE1
NCT05382338	RECRUITING	Childhood Medulloblastoma	Children's Oncology Group	2023-02-20	PHASE3
NCT06177964	RECRUITING	Recurrent Supratentorial Glioblastoma	Darell Bigner	2024-07-15	PHASE2
NCT06325683	SUSPENDED	Progressive Glioblastoma; Recurrent Glioblastoma	National Cancer Institute (NCI)	2024-11-08	PHASE2
NCT07632209	NOT_YET_RECRUITING	SCLC, Extensive Stage	Yayi He	2026-06	PHASE2

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Handling Instructions

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Lomustine DNA/RNA Synthesis chemical

Chemical Structure

Molecular Weight: 233.7