Fludarabine Phosphate

For research use only.

Catalog No.S1229 Synonyms: F-ara-A (NSC 312887) Phosphate

41 publications

Fludarabine Phosphate Chemical Structure

CAS No. 75607-67-9

Fludarabine Phosphate (F-ara-A, NSC 312887) is an analogue of adenosine and deoxyadenosine, which is able to compete with dATP for incorporation into DNA and inhibit DNA synthesis.

Selleck's Fludarabine Phosphate has been cited by 41 publications

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Fludarabine Phosphate (F-ara-A, NSC 312887) is an analogue of adenosine and deoxyadenosine, which is able to compete with dATP for incorporation into DNA and inhibit DNA synthesis.
DNA polymerase α [1]
(Cell-free assay)
DNA polymerase δ [1]
(Cell-free assay)
1.1 μM(Ki ) 1.3 μM(Ki)
In vitro

Fludarabine Phosphate is converted to F-ara-ATP in cells and then incorporated into DNA in a self-limiting manner. Fludarabine Phosphate competes with dATP for incorporation into the A site of the extending DNA strand, which results in termination of DNA strand elongation. Human DNA polymerase α incorporates more Fludarabine Phosphate into DNA than polymerase δ. Fludarabine Phosphate completively inhibits DNA polymerase α and DNA polymerase δ with Ki of 1.1 μM and 1.3 μM, respectively. DNA polymerase δ is also able to excise the incorporated Fludarabine Phosphate from DNA in vitro. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HL60 cells NVfpS|NuWHKxbHnm[ZJifGmxbjDhd5NigQ>? M3z5N|Q5KGh? NX;rdW17SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHyg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkC5JO69VQ>? NYPrPGt{OjV7NkCzNlM>
MCF7 cells NWewb5lwWHKxbHnm[ZJifGmxbjDhd5NigQ>? M1npR|Q5KGh? NVi4RYllSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGOnbHyg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkGzJO69VQ>? M33rN|I2QTZyM{Kz
KG1 cells M33LZXBzd2yrZnXyZZRqd25iYYPzZZk> MnuwOFghcA>? M1KzU2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iS1exJINmdGy|IHHzd4V{e2WmIHHzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjF3IN88US=> MoTsNlU6PjB|MkO=
Raji cells M3L5OHBzd2yrZnXyZZRqd25iYYPzZZk> Mn61OFghcA>? NXP1V2VySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDSZYpqKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHyg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkSg{txO MWCyOVk3ODN{Mx?=
K562 cells MnHmVJJwdGmoZYLheIlwdiCjc4PhfS=> NY\DRlRxPDhiaB?= Mki1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNNU[yJINmdGy|IHHzd4V{e2WmIHHzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjRizszN M2rRNFI2QTZyM{Kz
ZR-75-1 cells MWjQdo9tcW[ncnH0bY9vKGG|c3H5 M4rrVVQ5KGh? M1;ibGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iWmKtO|UuOSClZXzsd{Bie3Onc4Pl[EBieyClZXzsJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD62JO69VQ>? MVqyOVk3ODN{Mx?=
MOLT3 cells M2fVS3Bzd2yrZnXyZZRqd25iYYPzZZk> M360cFQ5KGh? MnT2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPT1zUN{Bk\WyuczDhd5Nme3OnZDDhd{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC57NTFOwG0> MWiyOVk3ODN{Mx?=
M-HeLa cells MoTSVJJwdGmoZYLheIlwdiCjc4PhfS=> MVi0PEBp MoD6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPLVjlUIEh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTJizszN M4rob|I2QTZyM{Kz
SK-UT-1B cells NGCyTpdRem:uaX\ldoF1cW:wIHHzd4F6 NGfES|A1QCCq NF[5OYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMXVVNTGEIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME22JO69VQ>? NXPVOFY2OjV7NkCzNlM>

... Click to View More Cell Line Experimental Data

In vivo Fludarabine Phosphate is toxic for tumor-free mice. The maximum tolerated dose (LD10) Fludarabine Phosphate administered as a single dose is 234 mg/kg. The 50% lethal dose is 375 mg/kg. Fludarabine Phosphate administered as a single dose induces fewer number of cells surviving therapy in mice bearing P388 leukemia, accompanied by greater percentage of increase in life span (110%) and increased median survival time. [3]


Cell Research:[1]
- Collapse
  • Cell lines: Human T lymphoblastoid cells
  • Concentrations: 30 μM
  • Incubation Time: 5 hours
  • Method: Cells are incubated with Fludarabine Phosphate for 5 hr and washed twice with drug-free warm medium. 800 cells are mixed with 1.3 mL of 0.25% soft agar in Dulbecco’s medium supplemented with 20% fetal bovine serum (pre-warmed to 37 ℃) and incubated in a tissue culture dish for 10 days (humidified 5% CO2 , 37 ℃). At the end of the incubation period, colonies of more than 40 cells are scored under a microscope. The cytotoxic effect of the drugs is expressed as a percentage of survival relative to that of untreated control cells.
    (Only for Reference)
Animal Research:[3]
- Collapse
  • Animal Models: Murine Leukemia P3881
  • Dosages: 234 mg/kg
  • Administration: A single dose i.p. at day 1
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 73 mg/mL (199.88 mM)
Water 2 mg/mL warmed (5.47 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 365.21


CAS No. 75607-67-9
Storage powder
in solvent
Synonyms F-ara-A (NSC 312887) Phosphate
Smiles C1=NC2=C(N=C(N=C2N1C3C(C(C(O3)COP(=O)(O)O)O)O)F)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03878953 Not yet recruiting Drug: rhPTH(1-84) Chronic Hypoparathyroidism Shire|Takeda March 31 2022 Phase 3
NCT04763499 Not yet recruiting Other: freeze dried strawberry powder Vascular Dilation University of California Davis October 1 2021 Not Applicable
NCT04485039 Not yet recruiting Drug: TPOXX Smallpox SIGA Technologies|Biomedical Advanced Research and Development Authority|PPD September 15 2021 Phase 4
NCT04657146 Not yet recruiting Other: Biorepository Glioblastoma Duke University September 1 2021 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

DNA/RNA Synthesis Signaling Pathway Map

Related DNA/RNA Synthesis Products

Tags: buy Fludarabine Phosphate | Fludarabine Phosphate supplier | purchase Fludarabine Phosphate | Fludarabine Phosphate cost | Fludarabine Phosphate manufacturer | order Fludarabine Phosphate | Fludarabine Phosphate distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID