SRT1720 HCl

Catalog No.S1129

SRT1720 HCl Chemical Structure

Molecular Weight(MW): 506.02

SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3.

Size Price Stock Quantity  
In DMSO USD 238 In stock
USD 170 In stock
USD 320 In stock
USD 970 In stock
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5 Customer Reviews

  • EMBO J 2013 32, 791-804. SRT1720 HCl purchased from Selleck.

    PAI-1 expression in HUVECs treated with drugs as indicated.(D) senescent HUVECs were treated with SRT1720, culturing for 24, 48 hours. PAI-1 mRNA and protein (E) levels were analyzed using real-time RT–PCR and Western blotting, respectively. The RNA and protein levels were normalized to the internal control β-actin. Data are presented as the mean±SEM of three independent experiments. *P < 0.05 vs. corresponding control. **P < 0.01 vs. corresponding control ***P < 0.001 vs. corresponding control.

    Aging Cell 2014 13(5), 890-9. SRT1720 HCl purchased from Selleck.

  • Sirt1 deacetylase activity is essential for IRF9-mediated ischemic injury. The effects of SRT1720 on Sirt1 deacetylase activity in IRF9-KO and IRF9-TG mice, respectively. *p < 0.05 versus DMSO controls. n = 5.

    J Neurosci 2014 34(36), 11897-912. SRT1720 HCl purchased from Selleck.

    C2C12 myoblasts were transfected with si-CON and si-NDUFV1 for 24 h and then further differentiated into myotubes for 4 days in the absence or presence of pyruvate (25 mM), SRT1720 (2 uM), or resveratrol (Resv; 25 uM). Myogenesis was monitored using MyHC immunofluorescence and DAPI.

    J Biol Chem 2014 289(29), 20012-25. SRT1720 HCl purchased from Selleck.

  • J Biol Chem 2012 287, 19304-19314. SRT1720 HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Sirtuin Inhibitors

Biological Activity

Description SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3.
Targets
SIRT1 [1]
(Cell-free assay)
0.16 μM(EC50)
In vitro

The maximum activation ratio of SRT1720 versus the closest sirtuin homologues, SIRT2 (EC1.5 = 37 μM) and SIRT3 (EC1.5 > 300 μM) is up to 781%. SRT1720 binds to the SIRT1 enzyme-peptide substrate complex at an allosteric site amino-terminal to the catalytic domain and lower the Michaelis constant for acetylated substrates. SRT1720 could reduce fed glucose levels. Glucose excursion during an intraperitoneal glucose tolerance test is also significantly reduced in the SRT1720 group, and comparable to rosiglitazone, a PPARγ activator that has been used to treat type 2 diabetes. SRT1720 does not have an effect on fasting glucose in chow-fed mice, revealing that pharmacological SIRT1 activation is unlikely to induce hypoglycaemia. SRT1720 significantly reduces the hyperinsulinaemia after 4 weeks, partially normalizing increased insulin levels similar to rosiglitazone treatment. SRT1720 treatment increases mitochondrial capacity by 15% in gastrocnemius muscle as measured by citrate synthase activity. [1] Higher concentrations of SRT1720 (15 μM) induces a modest (10-20%) decrease in normal cell viability. SRT1720 also significantly inhibits VEGF-dependent MM cell migration. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CACs  NYrS[Ho5TnWwY4Tpc44hSXO|YYm= NWC3RZRoPMLizszN NHe1UZc{OMLibXnu MmT5SG1UVw>? M4fEWolv\HWlZYOgZYN2fGViU1nSWFEh[WO2aY\heIlwdsLi M{e3UlI3OjV2MUC0
MC3T3-E1 MnPZSpVv[3Srb36gRZN{[Xl? MWCxNEDDvU4EoB?= NYizOXU4OSCq NInlTWFz\WS3Y3XzJJRp\SCWR1[t{tIue3SrbYXsZZRm\CCYRVfGJJJmdGWjc3WgbY4h\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nctMg NUnGPJNoOjZzM{[5O|g>
MC3T3-E1 M{GybGZ2dmO2aX;uJGF{e2G7 NHfFRo8yOCEEtV5CpC=> NHi3N4oyOiCq M{\tVJJm\HWlZYOgeIhmKF[HR1[gcXJPSSCneIDy[ZN{cW:wIHzleoVteyC|dHnteYxifGWmIHL5JHRITi4Qsh?= M2LL[|I3OTN4OUe4
MC3T3-E1 M1nqbmZ2dmO2aX;uJGF{e2G7 NUP0UZp1OjBizszN NIjuPWoyKGh? MYDzeZBxemW|c3XzJJRp\SCWR1[t{tIucW6mdXPl[EBxcG:|cHjvdplt[XSrb36gc4YheDR2L4C0NkBOSVBia3nuZZNmKG:{IGPBVGswUk6N M3PXclI3OTN4OUe4
WE-68 MUnBdI9xfG:|aYOgRZN{[Xl? M4Pz[|AuOjRizszN MVKyOEBp Mn3xbY5lfWOnczDj[YxtKGSnYYToJIlvKGSxc3Wg[IVx\W6mZX70cJk> NUTsO2dwOjZyNUW4NFU>
SK-ES-1 MW\BdI9xfG:|aYOgRZN{[Xl? NWDUblJIOC1zMDFOwG0> M2rKc|I1KGh? NXPFVI9McW6mdXPld{Bk\WyuIHTlZZRpKGmwIHTvd4Uh\GWyZX7k[Y51dHl? MYGyOlA2PThyNR?=
SK-N-MC  NFfkZ5NCeG:ydH;zbZMhSXO|YYm= MoXUNE0zNjVizszN MWiyOEBp MmXqbY5lfWOnczDj[YxtKGSnYYToJIlvKGSxc3Wg[IVx\W6mZX70cJk> Mly2NlYxPTV6MEW=
WE-68 MoL4SpVv[3Srb36gRZN{[Xl? M3zpb|IxKM7:TR?= NVrWVWpuOC1{NDDo NXPXZW9p[WO2aY\heIV{KGOjc4Dhd4UhOy95 NWnKUG1nOjZyNUW4NFU>
SK-ES-1 NUHTU45jTnWwY4Tpc44hSXO|YYm= NX31dGlDOTBizszN NHfRfpQxNTJ2IHi= M2HTeYFkfGm4YYTld{Bk[XOyYYPlJFMwPw>? MmXGNlYxPTV6MEW=
SK-N-MC  MV\GeY5kfGmxbjDBd5NigQ>? MVmzJO69VQ>? MlfENE0zPCCq NFHhO2Ji[3SrdnH0[ZMh[2G|cHHz[UA{Nzd? MXOyOlA2PThyNR?=
NRK-49F MWfGeY5kfGmxbjDBd5NigQ>? NGfrRVYx6oDVMtMg{txO MkHBN|YhcA>? NGnReppqdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZjFOtU1UVUFiYX7kJIZq[nKxbnXjeIlvKGSxc3Wg[IVx\W6mZX70cJk> M{S3fFI3ODJ{MECz
NRK-49F NFHlXJhHfW6ldHnvckBCe3OjeR?= M1X0SlDjiJN{wrFOwG0> NInSTWc{PiCq Mn7D[Y5p[W6lZYOgdIhwe3Cqb4L5cIF1cW:wIH;mJGVITlJiYX7kJHBFT0[UzsNCpC=> MU[yOlAzOjByMx?=
NRK-49F MXXGeY5kfGmxbjDBd5NigQ>? MUKw5qCUOsLizszN MUezOkBp NWLCNnlx\W6qYX7j[ZMhW1SDVEOgdIhwe3Cqb4L5cIF1cW:w Mkj6NlYxOjJyMEO=
RAW264.7 M3nBSWZ2dmO2aX;uJGF{e2G7 MlXHNUDPxE1? Mn7KOkBp MoDQeZBz\We3bHH0[ZMhfGinIILl[JVk\WRiU1nSWFEheHKxdHXpckBweiCvUl7BJIxmfmWuczDifUBpcWeqIHfseYNwe2V? NIL0e3kzPTd7M{m5OS=>
MCF10A MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYmwMVIxKM7:TR?= M3zyZlI1KGh? MXfy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NF:1[YIzPTRzMUO1Oi=>
MCF-7 NI\3UI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLvNE0zOCEQvF2= NFHXWpUzPCCq MmXIdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M2jaTFI2PDFzM{W2
T47D M4G0d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPsNE0zOCEQvF2= Ml;xNlQhcA>? NWjj[3dlemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 M1W1VVI2PDFzM{W2
SKBR3 NUT1NGJMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mor1NE0zOCEQvF2= MVuyOEBp NUPWU49uemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MmLDNlU1OTF|NU[=
MDA-MB-231 M3;YWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV6wMVIxKM7:TR?= MUmyOEBp MnzBdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NFfzXo0zPTRzMUO1Oi=>
SUM149 NX7TNph5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWKwMVIxKM7:TR?= NVPZWVI4OjRiaB?= NGnkV4pz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M1Hq[|I2PDFzM{W2
HS578T MmLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnT3NE0zOCEQvF2= MUSyOEBp M13LS5Jm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? M2PVUFI2PDFzM{W2
BT20 NEjwbXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfUTFdOOC1{MDFOwG0> M{jMNVI1KGh? MWry[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> M{e2NFI2PDFzM{W2
A459 NHrlPZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLSOWwxNTJyIN88US=> NXu2NGNZOjRiaB?= M3TwZ5Jm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? M4T0e|I2PDFzM{W2
HCT116 NVnpSXk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTrNE0zOCEQvF2= M3zBUFI1KGh? NIT1Snpz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M2LwSlI2PDFzM{W2
Neu M3fLPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWL6WFJQOC1{MDFOwG0> NXfuRoNnOjRiaB?= MWry[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NI[4TIIzPTRzMUO1Oi=>
MDA-MB-231 MWHGeY5kfGmxbjDBd5NigQ>? MV[1JO69VQ>? MlzyPEBp NUnOZ2RpcW6lcnXhd4V{KHSqZTDueY1j\XJib3[gZYNq\GmlII\ld4lkfWyjcjDvdodidmWubHXz M2XDUFI2PDFzM{W2
MDA-MB-231 NHizd|lHfW6ldHnvckBCe3OjeR?= MWS1JO69VQ>? MnPsNVYhcA>? NYX3VW1lcW6mdXPld{BtgXOxc3;tZYwhdWWvYoLhcoUheGW{bXXhZoltcXqjdHnvci=> NEfNd28zPTRzMUO1Oi=>
MC3T3-E1 MXjGeY5kfGmxbjDBd5NigQ>? MoOxNVAh|ryP M1WxS|YxKG2rbtMg NUHLR5M2e3WycILld5NmeyC2aHWgSmdHNTJvc4TpcZVt[XSnZDDvd5Rmd3C{b4Tl[4VzcW5icnXs[YF{\Q>? MXiyOVI6ODB7NR?=
MC3T3-E1 MnPHSpVv[3Srb36gRZN{[Xl? M3PyW|ExKM7:TR?= MXq2NEBucW8EoB?= MVnheJRmdnWjdHXzJJRp\SCIR1[tNk1qdmS3Y3XkJI9{fGWxcILveIVo\XKrbjDtVm5CKGW6cILld5Nqd25? M3[wT|I2OjlyMEm1
MC3T3-E1 M{ftSGZ2dmO2aX;uJGF{e2G7 NF\VNIMyOCEQvF2= M4HleVYxKG2rbtMg MorsZZR1\W63YYTld{B1cGViRlfGMVIucW6mdXPl[EBwe3Snb4Dyc5Rm\2W{aX6gcXJPSSCneIDy[ZN{cW:w M3r1dlI2OjlyMEm1
MC3T3-E1 MkTVSpVv[3Srb36gRZN{[Xl? MkjHNVAh|ryP MkWwOlAhdWmwwrC= NI\Od3B{fXCycnXzd4V{KHSqZTDCUXAuPC2|dHnteYxifGWmIG\FS2YhemWuZXHz[S=> NXzv[VZWOjR2M{W0OFQ>
MC3T3-E1 NXfQfmIxTnWwY4Tpc44hSXO|YYm= M1[5ZVExKM7:TR?= M2W0SVYxKG2rbtMg MljGd5VxeHKnc4Pld{B1cGViUFfGNu6yNXO2aX31cIF1\WRiT2DHJJJmdGWjc3W= NWOyN|U2OjR|M{OzN|Y>
MC3T3-E1 MXvGeY5kfGmxbjDBd5NigQ>? MWCxNEDPxE1? NWroOVBSPjBibXnuxsA> NHraW3Nz\WS3Y3XzJJRp\SCSR1[y{tEue3SrbYXsZZRm\CCyaH;zdIhwenmuYYTpc44hd2ZicES0M5A1OiCPQWCgb4lv[XOn MnzQNlQ{OzN|M{[=
MC3T3-E1 NXrYNGdFTnWwY4Tpc44hSXO|YYm= Ml73NVAh|ryP MVu2NEBucW8EoB?= NFO0ZohifHSnboXheIV{KHSqZTDQS2Yz|rFvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiYn;0bEBOTUtzL{KgZY5lKFKjZj2x M4fBXFI1OzN|M{O2
RPE NFjMcmZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M4rmcFUhyrWP NW[yc|A4OSCq NFTVdFZifHSnboXheIV{KE:DzsKtbY5lfWOnZDDk[YNz\WG|ZTDv[kBk\WyuII\pZYJqdGm2eR?= MXiyOFA{Pjl|OB?=
9607 MV3D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHywTGUyKM7:TR?= MYezOkBp M{HNbYlv[3KnYYPld{B1cGViY3XscEB3cWGkaXzpeJkh[2:vcHHy[YQhf2m2aDDt[YxifG:waX6gZYxwdmV? NHz2UHEzOzd{Nkm0PS=>
9607 NX;kcoNKTnWwY4Tpc44hSXO|YYm= NYr6[GR2OSEQvF2= M{XSVFM3KGh? NXO5fYlCcW6lcnXhd4V{KFOLUmSxJIFv\CCmZXPy[YF{\WRiYXPleJlt[XSnZD3wOVMh\XiycnXzd4lwdg>? MliwNlM4OjZ7NEm=
RPMI.8226 NX7LbItiS2WubDDWbYFjcWyrdImgRZN{[Xl? NYfleIFLPy9zMDFOwG0> NFe2PWczPCCq MkL0[IVkemWjc3XzJJZq[WKrbHn0fUBkd26lZX70doF1cW:wIHTldIVv\GWwdHz5 Mlf0NlE6PTB5Mki=
U266 NWe1TolIS2WubDDWbYFjcWyrdImgRZN{[Xl? NFXZTJM4NzFyIN88US=> MY[yOEBp M1;RT4Rm[3KnYYPld{B3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> NFXsbpEzOTl3MEeyPC=>
MM.1S NGDublZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MoXIO{8yOCEQvF2= NG\6VpEzPCCq MYnk[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= NYjaTmZWOjF7NUC3Nlg>
KMS12 MnrUR4VtdCCYaXHibYxqfHliQYPzZZk> M4\kVVcwOTBizszN NWDHS2hwOjRiaB?= NEe5R2Zl\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NUDYR2VVOjF7NUC3Nlg>
LR5 NVq0b5hXS2WubDDWbYFjcWyrdImgRZN{[Xl? MV23M|ExKM7:TR?= MXGyOEBp NYDsXpV4\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 NYTGO4VCOjF7NUC3Nlg>
MM.1R MVfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHTUPI44NzFyIN88US=> NFnuboUzPCCq NIPKU3Fl\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> MnHMNlE6PTB5Mki=
Ina6 MofKR4VtdCCYaXHibYxqfHliQYPzZZk> MY[3M|ExKM7:TR?= NFnvTpozPCCq NXKzb5Ru\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 NE\SNHIzOTl3MEeyPC=>
RPMI-8226 NHu0UphCeG:ydH;zbZMhSXO|YYm= MVu3M|ExKM7:TR?= NGDLSowzPCCq MlPMbY5lfWOnczDhJJNq\26rZnnjZY51KGmwY4LlZZNmKGmwIITo[UBCdm6neHnuJHYsN1CL4pkSxsBieG:ydH;zbZM> NFXSd5kzOTl3MEeyPC=>
MM.1R  NVjnOZB5SXCxcITvd4l{KEG|c3H5 NIfF[VA4NzFyIN88US=> MYiyOEBp NX7RVGdTcW6mdXPld{BiKHOrZ37p[olk[W62IHnuZ5Jm[XOnIHnuJJRp\SCDbn7lfIlvKFZtL2DJ5qiTyqCjcH;weI9{cXN? MnrPNlE6PTB5Mki=
H411EC3 NULl[mY2TnWwY4Tpc44hSXO|YYm= NEXi[402OC9zMECgcm0> NIPXSGI3KGh? NVnVOWRKcW6lcnXhd4V{KFOLUmSxJIFkfGm4aYT5JIlvKHSqZTDwdoV{\W6lZTDv[kBVW0FuIGDFVGNMKGGldHn2bZR6NCCvUl7BJIxmfmWuczDv[kBR[2tzIHHu[EBR\2NzzsGsJIFv\CCnbHX2ZZRqdmdiZ3z1Z49{\SCycn;keYN1cW:w NXjLVnVwOjF{MUKwPVY>
hepatocytes MnXlSpVv[3Srb36gRZN{[Xl? Mlu5NVAhdk1? NHvTWIc3KGh? NWD6Nlg6cW6lcnXhd4V{KFOLUmSxJIFkfGm4aYT5JIlvKHSqZTDwdoV{\W6lZTDv[kBVW0FuIGDFVGNMKGGldHn2bZR6NCCvUl7BJIxmfmWuczDv[kBR[2tzIHHu[EBR\2NzzsGsJIFv\CCnbHX2ZZRqdmdiZ3z1Z49{\SCycn;keYN1cW:w NHfZOYgzOTJzMkC5Oi=>
hepatocytes NGnKcmtHfW6ldHnvckBCe3OjeR?= MmfKNVAhdk1? MWK2JIg> NFO1UGRqdmO{ZXHz[ZMhUG2pY4NCpIFv\MLiQXPjxsBo\W6nIHX4dJJme3Orb36= M2\vOlIyOjF{MEm2

... Click to View More Cell Line Experimental Data

In vivo In DIO mice SRT1720 mimics several of the effects observed after calorie restriction including improved insulin sensitivity, normalized glucose and insulin levels, and increased mitochondrial capacity. In addition, in diet-induced obese and genetically obese mice, SRT1720 improves insulin sensitivity, lower plasma glucose, and increase mitochondrial capacity. Thus, SRT1720 is a promising new therapeutic agent for treating diseases of ageing such as type 2 diabetes. Consistent with improved glucose tolerance, the glucose infusion rate required to maintain euglycaemia is approximately 35% higher in SRT1720-treated fa/fa rats, and the total glucose disposal rate is increased by approximately 20%. [1] SRT1720 also prevents multiple myeloma tumor growth. SRT1720 increases the cytotoxic activity of bortezomib or dexamethasone. [2]

Protocol

Kinase Assay:[1]
+ Expand

SIRT1 fluorescence polarization assay:

In the SIRT1 FP assay, SIRT1 activity is monitored using a 20 amino acid peptide (Ac-Glu-Glu-Lys(biotin)-Gly-Gln-Ser-Thr-Ser-Ser-His-Ser-Lys(Ac)-Nle-Ser-Thr-Glu-Gly–Lys(MR121 or Tamra)-Glu-Glu-NH2) derived from the sequence of p53. The peptide is N-terminally linked to biotin and C-terminally modified with a fluorescent tag. The reaction for monitoring enzyme activity is a coupled enzyme assay where the first reaction is the deacetylation reaction catalyzed by SIRT1 and the second reaction is cleavage by trypsin at the newly exposed lysine residue. The reaction is stopped and streptavidin is added in order to accentuate the mass differences between substrate and product. The sensitivity of the FP assay allows identification of SRT1720. The fluorescence polarization reaction conditions are as follows: 0.5 μM peptide substrate, 150 μM βNAD+, 0-10 nM SIRT1, 25 mM Tris-acetate pH 8, 137 mM Na-Ac, 2.7 mM K-Ac, 1 mM Mg-Ac, 0.05% Tween-20, 0.1% Pluronic F127, 10 mM CaCl 2, 5 mM DTT, 0.025% BSA, and 0.15 mM nicotinamide. The reaction is incubated at 37 °C and stopped by addition of nicotinamide, and trypsin is added to cleave the deacetylated substrate. This reaction is incubated at 37 °C in the presence of 1 μM streptavidin. Fluorescent polarization is determined at excitation (650 nm) and emission (680 nm) wavelengths.
Cell Research:[2]
+ Expand
  • Cell lines: Human vascular endothelial cells (HUVECs)
  • Concentrations: 5 μM
  • Incubation Time: 2 hours
  • Method: Transwell Insert Assays are utilized to measure migration. In vitro angiogenesis is assessed by Matrigel capillary-like tube structure formation assay. For endothelial tube formation assay, human vascular endothelial cells (HUVECs) are obtained from Clonetics and maintained in endothelial cell growth medium-2 containing 5% FBS. After three passages, HUVEC cell viability is measured with the trypan blue exclusion assay, and <5% of cell death is observed with SRT1720 treatment.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Chase-SCID mice with MM.1S cells
  • Formulation: 20% PEG400/0.5% Tween80/79.5% deionized water
  • Dosages: 200 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 38 mg/mL (75.09 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% Propylene glycol
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 506.02
Formula

C25H23N7OS.HCl

CAS No. 1001645-58-4
Storage powder
in solvent
Synonyms N/A

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  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How can we prepare Srt1720 for in vivo mouse studies?

  • Answer:

    SRT1720 HCl can be dissolved in 30% PEG 400+0.5% Tween 80+5% Propylene glycol at 30mg/ml as a suspension. It is fine for oral gavage. And we’ve also found that it can be dissolved in 2% DMSO+30% PEG 300+1%Tween 80+ddH2O at 3mg/ml clearly, which could be used for injection. When prepare the solution, please dissolve the compound in DMSO clearly first, then add PEG and Tween. After they mixed well, dilute with water.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID