SRT1720 HCl

For research use only.

Catalog No.S1129

116 publications

SRT1720 HCl Chemical Structure

CAS No. 1001645-58-4

SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3. SRT1720 induces autophagy.

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Selleck's SRT1720 HCl has been cited by 116 publications

Purity & Quality Control

Choose Selective Sirtuin Inhibitors

Biological Activity

Description SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3. SRT1720 induces autophagy.
Targets
SIRT1 [1]
(Cell-free assay)
0.16 μM(EC50)
In vitro

The maximum activation ratio of SRT1720 versus the closest sirtuin homologues, SIRT2 (EC1.5 = 37 μM) and SIRT3 (EC1.5 > 300 μM) is up to 781%. SRT1720 binds to the SIRT1 enzyme-peptide substrate complex at an allosteric site amino-terminal to the catalytic domain and lower the Michaelis constant for acetylated substrates. SRT1720 could reduce fed glucose levels. Glucose excursion during an intraperitoneal glucose tolerance test is also significantly reduced in the SRT1720 group, and comparable to rosiglitazone, a PPARγ activator that has been used to treat type 2 diabetes. SRT1720 does not have an effect on fasting glucose in chow-fed mice, revealing that pharmacological SIRT1 activation is unlikely to induce hypoglycaemia. SRT1720 significantly reduces the hyperinsulinaemia after 4 weeks, partially normalizing increased insulin levels similar to rosiglitazone treatment. SRT1720 treatment increases mitochondrial capacity by 15% in gastrocnemius muscle as measured by citrate synthase activity. [1] Higher concentrations of SRT1720 (15 μM) induces a modest (10-20%) decrease in normal cell viability. SRT1720 also significantly inhibits VEGF-dependent MM cell migration. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CACs  NU\aZ|dWTnWwY4Tpc44hSXO|YYm= M{LSeVTDqM7:TR?= NWjmPXFOOzEEoH3pci=> NIrS[|RFVVOR M2rjXYlv\HWlZYOgZYN2fGViU1nSWFEh[WO2aY\heIlwdsLi M4rXOVI3OjV2MUC0
MC3T3-E1 MX7GeY5kfGmxbjDBd5NigQ>? NH3EUVUyOCEEtV5CpC=> MYOxJIg> M3XL[5Jm\HWlZYOgeIhmKFSJRj5Otk1{fGmvdXzheIVlKF[HR1[gdoVt\WG|ZTDpckBld3OnLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{wrC= NXfCWZBFOjZzM{[5O|g>
MC3T3-E1 M2HHVWZ2dmO2aX;uJGF{e2G7 MofQNVAhyrWPwrC= NXzaUXpDOTJiaB?= NHuzUIJz\WS3Y3XzJJRp\SCYRVfGJI1TVkFiZYjwdoV{e2mxbjDs[ZZmdHNic4TpcZVt[XSnZDDifUBVT0ZvzsK= MmOxNlYyOzZ7N{i=
MC3T3-E1 M37JPWZ2dmO2aX;uJGF{e2G7 M3\aWFIxKM7:TR?= NFjFVGUyKGh? Mn2yd5VxeHKnc4Pld{B1cGViVFfGMe6zNWmwZIXj[YQheGixc4Doc5J6dGG2aX;uJI9nKHB2ND;wOFIhVUGSIHvpcoF{\SCxcjDTRXBMN0qQSx?= Mk\6NlYyOzZ7N{i=
WE-68 NUe0XWdiSXCxcITvd4l{KEG|c3H5 M1Xn[VAuOjRizszN NIja[JczPCCq MkXBbY5lfWOnczDj[YxtKGSnYYToJIlvKGSxc3Wg[IVx\W6mZX70cJk> MnLrNlYxPTV6MEW=
SK-ES-1 NUDlU3kxSXCxcITvd4l{KEG|c3H5 MWWwMVExKM7:TR?= NGTnfZQzPCCq MXjpcoR2[2W|IHPlcIwh\GWjdHigbY4h\G:|ZTDk[ZBmdmSnboTsfS=> NH\JTZQzPjB3NUiwOS=>
SK-N-MC  NFrsR4dCeG:ydH;zbZMhSXO|YYm= M1nPT|AuOi53IN88US=> NIjNVoszPCCq NHTDPGFqdmS3Y3XzJINmdGxiZHXheIghcW5iZH;z[UBl\XCnbnTlcpRtgQ>? NHXxV2ozPjB3NUiwOS=>
WE-68 NIC2VFBHfW6ldHnvckBCe3OjeR?= NELuWoMzOCEQvF2= M{\sO|AuOjRiaB?= NET2c3Ni[3SrdnH0[ZMh[2G|cHHz[UA{Nzd? MoP2NlYxPTV6MEW=
SK-ES-1 M2jWfWZ2dmO2aX;uJGF{e2G7 M{DkWVExKM7:TR?= NWHaVVdvOC1{NDDo NEi5UFhi[3SrdnH0[ZMh[2G|cHHz[UA{Nzd? NXSyTnk3OjZyNUW4NFU>
SK-N-MC  NYO5XGJXTnWwY4Tpc44hSXO|YYm= NFKzWFY{KM7:TR?= MmnRNE0zPCCq M1foXIFkfGm4YYTld{Bk[XOyYYPlJFMwPw>? MXmyOlA2PThyNR?=
NRK-49F MYnGeY5kfGmxbjDBd5NigQ>? NF\CTm0x6oDVMtMg{txO NY\FVZVUOzZiaB?= MlTObY5kemWjc3XzJIV5eHKnc4Ppc44hd2ZizsGtV21CKGGwZDDmbYJzd26nY4TpckBld3OnIHTldIVv\GWwdHz5 Mnn3NlYxOjJyMEO=
NRK-49F MVzGeY5kfGmxbjDBd5NigQ>? Mn3LNQKBmzMEoN88US=> NXrhVmJsOzZiaB?= M{nDNoVvcGGwY3XzJJBpd3OyaH;yfYxifGmxbjDv[kBGT0[UIHHu[EBRTEeIUt8yxsA> NHrpZlMzPjB{MkCwNy=>
NRK-49F NWPrPYl{TnWwY4Tpc44hSXO|YYm= MmfjNQKBmzMEoN88US=> MV:zOkBp M3fYPIVvcGGwY3XzJHNVSVR|IIDoc5NxcG:{eXzheIlwdg>? M1[2[FI3ODJ{MECz
RAW264.7 NVXDd3dQTnWwY4Tpc44hSXO|YYm= MV2xJO69VQ>? MWq2JIg> MX;1dJJm\3WuYYTld{B1cGVicnXkeYNm\CCVSWLUNUBxem:2ZXnuJI9zKG2UTlGgcIV3\Wy|IHL5JIhq\2hiZ3z1Z49{\Q>? NEO3T4ozPTd7M{m5OS=>
MCF10A NVHDXphtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzMZYdMOC1{MDFOwG0> M{XjcFI1KGh? MYfy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> Mny1NlU1OTF|NU[=
MCF-7 NVn6VolHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPONE0zOCEQvF2= MoC1NlQhcA>? MWXy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NV7VXYwyOjV2MUGzOVY>
T47D NXnnPXNZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX:wMVIxKM7:TR?= NGGy[2czPCCq M1W1UJJm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NW\ZdoNQOjV2MUGzOVY>
SKBR3 Ml\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV6wMVIxKM7:TR?= NVfVdY1SOjRiaB?= NVfuSXI5emWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MWKyOVQyOTN3Nh?=
MDA-MB-231 NYXDU5FHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrvb2VxOC1{MDFOwG0> MkTzNlQhcA>? NHXNc41z\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? NFruU|YzPTRzMUO1Oi=>
SUM149 NVWzdVJTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVWwMVIxKM7:TR?= NFLueZEzPCCq NYjNcHFoemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MU[yOVQyOTN3Nh?=
HS578T MmLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\MOYEzOC1{MDFOwG0> M2iySVI1KGh? NGnyeVZz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MXyyOVQyOTN3Nh?=
BT20 NYDOVXUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\5eo4xNTJyIN88US=> M2Ph[|I1KGh? M1\E[5Jm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? M3rlSVI2PDFzM{W2
A459 NWL4S4FyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\FNE0zOCEQvF2= NYTnNHpkOjRiaB?= NH7UdFZz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M4fyNlI2PDFzM{W2
HCT116 M{DPXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHQNE0zOCEQvF2= M1fWZ|I1KGh? MkHqdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MnHLNlU1OTF|NU[=
Neu MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYiyXXR7OC1{MDFOwG0> MoLTNlQhcA>? MkfXdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MUeyOVQyOTN3Nh?=
MDA-MB-231 M33obmZ2dmO2aX;uJGF{e2G7 Mn\wOUDPxE1? MYi4JIg> NUPuUXBjcW6lcnXhd4V{KHSqZTDueY1j\XJib3[gZYNq\GmlII\ld4lkfWyjcjDvdodidmWubHXz MlLJNlU1OTF|NU[=
MDA-MB-231 NUHJZYRUTnWwY4Tpc44hSXO|YYm= MW[1JO69VQ>? NEjIZYkyPiCq MVrpcoR2[2W|IHz5d49{d22jbDDt[Y1jemGwZTDw[ZJu\WGkaXzpfoF1cW:w MWCyOVQyOTN3Nh?=
MC3T3-E1 M4nIfGZ2dmO2aX;uJGF{e2G7 M33pfVExKM7:TR?= NVq3TWlSPjBibXnuxsA> NXLpR5Rte3WycILld5NmeyC2aHWgSmdHNTJvc4TpcZVt[XSnZDDvd5Rmd3C{b4Tl[4VzcW5icnXs[YF{\Q>? MYeyOVI6ODB7NR?=
MC3T3-E1 M4\lTWZ2dmO2aX;uJGF{e2G7 MUKxNEDPxE1? MmT3OlAhdWmwwrC= NIPv[mFifHSnboXheIV{KHSqZTDGS2YuOi2rbnT1Z4VlKG:|dHXvdJJwfGWpZYLpckBuWk6DIHX4dJJme3Orb36= MmT4NlUzQTByOUW=
MC3T3-E1 NEPwO3lHfW6ldHnvckBCe3OjeR?= MmeyNVAh|ryP NVfqZ3hbPjBibXnuxsA> NUW1VFlu[XS2ZX71ZZRmeyC2aHWgSmdHNTJvaX7keYNm\CCxc4Tlc5Bzd3SnZ3XybY4hdVKQQTDlfJBz\XO|aX;u NIrrNYMzPTJ7MEC5OS=>
MC3T3-E1 MmTVSpVv[3Srb36gRZN{[Xl? NF\1VmEyOCEQvF2= NEHkXVk3OCCvaX9CpC=> NYDCPFRpe3WycILld5NmeyC2aHWgRm1RNTRvc4TpcZVt[XSnZDDWSWdHKHKnbHXhd4U> M4L3NlI1PDN3NES0
MC3T3-E1 M1T5VmZ2dmO2aX;uJGF{e2G7 NWr6fYtnOTBizszN M2C2WVYxKG2rbtMg NHTTUWx{fXCycnXzd4V{KHSqZTDQS2Yz|rFvc4TpcZVt[XSnZDDPVGchemWuZXHz[S=> NV7oUIZNOjR|M{OzN|Y>
MC3T3-E1 NXn1eXRyTnWwY4Tpc44hSXO|YYm= MmHMNVAh|ryP MVS2NEBucW8EoB?= NHXvS2Vz\WS3Y3XzJJRp\SCSR1[y{tEue3SrbYXsZZRm\CCyaH;zdIhwenmuYYTpc44hd2ZicES0M5A1OiCPQWCgb4lv[XOn MV6yOFM{OzN|Nh?=
MC3T3-E1 MUDGeY5kfGmxbjDBd5NigQ>? M1LBSFExKM7:TR?= MnK3OlAhdWmwwrC= NFTZToZifHSnboXheIV{KHSqZTDQS2Yz|rFvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiYn;0bEBOTUtzL{KgZY5lKFKjZj2x MmL3NlQ{OzN|M{[=
RPE MUnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mn[1OUDDvU1? MX[xJIg> NIPSW5FifHSnboXheIV{KE:DzsKtbY5lfWOnZDDk[YNz\WG|ZTDv[kBk\WyuII\pZYJqdGm2eR?= NWezNJF[OjRyM{[5N|g>
9607 NH;TZ2JE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MX[xJO69VQ>? NIDyT|I{PiCq NHjjWlVqdmO{ZXHz[ZMhfGinIHPlcIwhfmmjYnnsbZR6KGOxbYDhdoVlKHerdHigcYVt[XSxbnnuJIFtd26n MYmyN|czPjl2OR?=
9607 M4PsW2Z2dmO2aX;uJGF{e2G7 M4jyWFEh|ryP MV2zOkBp NY\FfJFZcW6lcnXhd4V{KFOLUmSxJIFv\CCmZXPy[YF{\WRiYXPleJlt[XSnZD3wOVMh\XiycnXzd4lwdg>? M4S4NFI{PzJ4OUS5
RPMI.8226 Mm[1R4VtdCCYaXHibYxqfHliQYPzZZk> MU[3M|ExKM7:TR?= NIKw[|gzPCCq MYjk[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= Mn3SNlE6PTB5Mki=
U266 M4fHfWNmdGxiVnnhZoltcXS7IFHzd4F6 MnrLO{8yOCEQvF2= NFe4e2szPCCq M2jTXoRm[3KnYYPld{B3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> MW[yNVk2ODd{OB?=
MM.1S NGL1RVRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NIfBWIg4NzFyIN88US=> MX2yOEBp NHLWd5Zl\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NFP3U5czOTl3MEeyPC=>
KMS12 NVXCWlJbS2WubDDWbYFjcWyrdImgRZN{[Xl? MnTrO{8yOCEQvF2= MXqyOEBp MYjk[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= MXuyNVk2ODd{OB?=
LR5 NE\PNWNE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MoDzO{8yOCEQvF2= NEK0OXQzPCCq MV7k[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= NWrEfG9vOjF7NUC3Nlg>
MM.1R Mkj3R4VtdCCYaXHibYxqfHliQYPzZZk> NETLOpg4NzFyIN88US=> M3PxT|I1KGh? NVv3dGNO\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 MlG3NlE6PTB5Mki=
Ina6 MlvsR4VtdCCYaXHibYxqfHliQYPzZZk> NX62VXZHPy9zMDFOwG0> MVOyOEBp M1XYcIRm[3KnYYPld{B3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> NV;jfnpzOjF7NUC3Nlg>
RPMI-8226 MlfwRZBweHSxc3nzJGF{e2G7 NV7i[5UzPy9zMDFOwG0> M3[xS|I1KGh? M3TxSYlv\HWlZYOgZUB{cWewaX\pZ4FvfCCrbnPy[YF{\SCrbjD0bIUhSW6wZYjpckBXMy:SSfMIluKh[XCxcITvd4l{ NYm5TYpWOjF7NUC3Nlg>
MM.1R  M{K5TWFxd3C2b4Ppd{BCe3OjeR?= NUey[oZPPy9zMDFOwG0> M4PvUFI1KGh? MWnpcoR2[2W|IHGgd4lodmmoaXPhcpQhcW6lcnXhd4UhcW5idHjlJGFvdmW6aX6gWkswWEokiKNCpIFxd3C2b4Ppdy=> NFvOcpczOTl3MEeyPC=>
H411EC3 NEDrcFhHfW6ldHnvckBCe3OjeR?= M3rXZlUxNzFyMDDuUS=> M3fX[FYhcA>? M33QVIlv[3KnYYPld{BUUVKWMTDhZ5Rqfmm2eTDpckB1cGVicILld4Vv[2Vib3[gWHNCNCCSRWDDT{Bi[3Srdnn0fUwhdVKQQTDs[ZZmdHNib3[gVINsOSCjbnSgVIdkOc7zLDDhcoQh\WyndnH0bY5oKGeudXPvd4UheHKxZIXjeIlwdg>? MYCyNVIyOjB7Nh?=
hepatocytes NWDrVXFuTnWwY4Tpc44hSXO|YYm= MXixNEBvVQ>? MlPHOkBp MYjpcoNz\WG|ZYOgV2lTXDFiYXP0bZZqfHliaX6geIhmKHC{ZYPlcoNmKG:oIGTTRUwhWEWSQ1ugZYN1cX[rdImsJI1TVkFibHX2[Yx{KG:oIGDjb|Eh[W6mIGDnZ|HPuSxiYX7kJIVt\X[jdHnu[{BodHWlb4PlJJBzd2S3Y4Tpc44> MWKyNVIyOjB7Nh?=
hepatocytes NF3qTmxHfW6ldHnvckBCe3OjeR?= MnfrNVAhdk1? NXX3NZJCPiCq NFPIRXpqdmO{ZXHz[ZMhUG2pY4NCpIFv\MLiQXPjxsBo\W6nIHX4dJJme3Orb36= NIPK[JAzOTJzMkC5Oi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved-PARP-1 / Cleaved-caspase-3 / LC3-II / p62 / SIRT1; 

PubMed: 26655844     


SU86.86 cells were transfected with control siRNA, SIRT1 siRNA#1, or DBC1 siRNA. 24 hours after transfection, cells were re-plated and allowed to attach for 24 hours. Cells were then treated with vehicle (control) or 5 μM SRT1720 for 16 hours. Expression 䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙

26655844
Immunofluorescence
Cathepsin B; 

PubMed: 26655844     


SU86.86 cells were incubated with vehicle or 5 μM SRT1720 for 5 hours for LysoTracker Red labeling and 16 hours for cathepsin B immunofluorescence, followed by DAPI staining.

26655844
Growth inhibition assay
Cell viability ; 

PubMed: 25411356     


MTT viability assay of cancer cell lines treated with different doses of SRT1720 for 24 hours. 

25411356
In vivo In DIO mice SRT1720 mimics several of the effects observed after calorie restriction including improved insulin sensitivity, normalized glucose and insulin levels, and increased mitochondrial capacity. In addition, in diet-induced obese and genetically obese mice, SRT1720 improves insulin sensitivity, lower plasma glucose, and increase mitochondrial capacity. Thus, SRT1720 is a promising new therapeutic agent for treating diseases of ageing such as type 2 diabetes. Consistent with improved glucose tolerance, the glucose infusion rate required to maintain euglycaemia is approximately 35% higher in SRT1720-treated fa/fa rats, and the total glucose disposal rate is increased by approximately 20%. [1] SRT1720 also prevents multiple myeloma tumor growth. SRT1720 increases the cytotoxic activity of bortezomib or dexamethasone. [2]

Protocol

Kinase Assay:[1]
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SIRT1 fluorescence polarization assay:

In the SIRT1 FP assay, SIRT1 activity is monitored using a 20 amino acid peptide (Ac-Glu-Glu-Lys(biotin)-Gly-Gln-Ser-Thr-Ser-Ser-His-Ser-Lys(Ac)-Nle-Ser-Thr-Glu-Gly–Lys(MR121 or Tamra)-Glu-Glu-NH2) derived from the sequence of p53. The peptide is N-terminally linked to biotin and C-terminally modified with a fluorescent tag. The reaction for monitoring enzyme activity is a coupled enzyme assay where the first reaction is the deacetylation reaction catalyzed by SIRT1 and the second reaction is cleavage by trypsin at the newly exposed lysine residue. The reaction is stopped and streptavidin is added in order to accentuate the mass differences between substrate and product. The sensitivity of the FP assay allows identification of SRT1720. The fluorescence polarization reaction conditions are as follows: 0.5 μM peptide substrate, 150 μM βNAD+, 0-10 nM SIRT1, 25 mM Tris-acetate pH 8, 137 mM Na-Ac, 2.7 mM K-Ac, 1 mM Mg-Ac, 0.05% Tween-20, 0.1% Pluronic F127, 10 mM CaCl 2, 5 mM DTT, 0.025% BSA, and 0.15 mM nicotinamide. The reaction is incubated at 37 °C and stopped by addition of nicotinamide, and trypsin is added to cleave the deacetylated substrate. This reaction is incubated at 37 °C in the presence of 1 μM streptavidin. Fluorescent polarization is determined at excitation (650 nm) and emission (680 nm) wavelengths.
Cell Research:[2]
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  • Cell lines: Human vascular endothelial cells (HUVECs)
  • Concentrations: 5 μM
  • Incubation Time: 2 hours
  • Method: Transwell Insert Assays are utilized to measure migration. In vitro angiogenesis is assessed by Matrigel capillary-like tube structure formation assay. For endothelial tube formation assay, human vascular endothelial cells (HUVECs) are obtained from Clonetics and maintained in endothelial cell growth medium-2 containing 5% FBS. After three passages, HUVEC cell viability is measured with the trypan blue exclusion assay, and <5% of cell death is observed with SRT1720 treatment.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Chase-SCID mice with MM.1S cells
  • Dosages: 200 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 38 mg/mL (75.09 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 506.02
Formula

C25H23N7OS.HCl

CAS No. 1001645-58-4
Storage powder
in solvent
Synonyms N/A
Smiles C1CN(CCN1)CC2=CSC3=NC(=CN23)C4=CC=CC=C4NC(=O)C5=NC6=CC=CC=C6N=C5.Cl

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How can we prepare Srt1720 for in vivo mouse studies?

  • Answer:

    SRT1720 HCl can be dissolved in 30% PEG 400+0.5% Tween 80+5% Propylene glycol at 30mg/ml as a suspension. It is fine for oral gavage. And we’ve also found that it can be dissolved in 2% DMSO+30% PEG 300+1%Tween 80+ddH2O at 3mg/ml clearly, which could be used for injection. When prepare the solution, please dissolve the compound in DMSO clearly first, then add PEG and Tween. After they mixed well, dilute with water.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID