SRT1720 HCl

For research use only.

Catalog No.S1129

117 publications

SRT1720 HCl Chemical Structure

CAS No. 1001645-58-4

SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3. SRT1720 induces autophagy.

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10mM (1mL in DMSO) RMB 1960.55 In stock
RMB 1400.82 In stock
RMB 2626.37 In stock
RMB 7964.73 In stock
RMB 20229.30 In stock
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Selleck's SRT1720 HCl has been cited by 117 publications

Purity & Quality Control

Choose Selective Sirtuin Inhibitors

Biological Activity

Description SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3. SRT1720 induces autophagy.
Targets
SIRT1 [1]
(Cell-free assay)
0.16 μM(EC50)
In vitro

The maximum activation ratio of SRT1720 versus the closest sirtuin homologues, SIRT2 (EC1.5 = 37 μM) and SIRT3 (EC1.5 > 300 μM) is up to 781%. SRT1720 binds to the SIRT1 enzyme-peptide substrate complex at an allosteric site amino-terminal to the catalytic domain and lower the Michaelis constant for acetylated substrates. SRT1720 could reduce fed glucose levels. Glucose excursion during an intraperitoneal glucose tolerance test is also significantly reduced in the SRT1720 group, and comparable to rosiglitazone, a PPARγ activator that has been used to treat type 2 diabetes. SRT1720 does not have an effect on fasting glucose in chow-fed mice, revealing that pharmacological SIRT1 activation is unlikely to induce hypoglycaemia. SRT1720 significantly reduces the hyperinsulinaemia after 4 weeks, partially normalizing increased insulin levels similar to rosiglitazone treatment. SRT1720 treatment increases mitochondrial capacity by 15% in gastrocnemius muscle as measured by citrate synthase activity. [1] Higher concentrations of SRT1720 (15 μM) induces a modest (10-20%) decrease in normal cell viability. SRT1720 also significantly inhibits VEGF-dependent MM cell migration. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CACs  NEK4NXhHfW6ldHnvckBCe3OjeR?= MVq0xsDPxE1? Mo\GN|DDqG2rbh?= M4r4PWROW09? Mn:5bY5lfWOnczDhZ5V1\SCVSWLUNUBi[3SrdnH0bY9vyqB? NXLPTndtOjZ{NUSxNFQ>
MC3T3-E1 MorOSpVv[3Srb36gRZN{[Xl? NXfpelJ{OTBiwsXNxsA> NGLB[GkyKGh? MV3y[YR2[2W|IITo[UBVT0ZvzsKtd5RqdXWuYYTl[EBXTUeIIILlcIVie2ViaX6g[I9{\S1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5mesLi MljqNlYyOzZ7N{i=
MC3T3-E1 MnPGSpVv[3Srb36gRZN{[Xl? NXSwR3hMOTBiwsXNxsA> M2jYWFEzKGh? Ml\odoVlfWOnczD0bIUhXkWJRjDtVm5CKGW6cILld5Nqd25ibHX2[Yx{KHO2aX31cIF1\WRiYomgWGdHNc7{ NFfCTGgzPjF|Nkm3PC=>
MC3T3-E1 MWPGeY5kfGmxbjDBd5NigQ>? NH3qSIMzOCEQvF2= NX:2UndiOSCq M{\tb5N2eHC{ZYPz[ZMhfGinIGTHSk3Pui2rbnT1Z4VlKHCqb4PwbI9zgWyjdHnvckBw\iCyNESvdFQzKE2DUDDrbY5ie2Vib4KgV2FRUy:MTlu= NYfvblFuOjZzM{[5O|g>
WE-68 NV3BPZBqSXCxcITvd4l{KEG|c3H5 MYCwMVI1KM7:TR?= NGSwWIQzPCCq M{TObIlv\HWlZYOgZ4VtdCCmZXH0bEBqdiCmb4PlJIRmeGWwZHXueIx6 MnXkNlYxPTV6MEW=
SK-ES-1 M3PKXGFxd3C2b4Ppd{BCe3OjeR?= M2D6V|AuOTBizszN NETlWYMzPCCq NGXwRoZqdmS3Y3XzJINmdGxiZHXheIghcW5iZH;z[UBl\XCnbnTlcpRtgQ>? M3zsOlI3ODV3OEC1
SK-N-MC  NY\KPXhySXCxcITvd4l{KEG|c3H5 MXiwMVIvPSEQvF2= Ml7qNlQhcA>? M1zoRYlv\HWlZYOgZ4VtdCCmZXH0bEBqdiCmb4PlJIRmeGWwZHXueIx6 M1zDVlI3ODV3OEC1
WE-68 NWjneGtETnWwY4Tpc44hSXO|YYm= M2PyN|IxKM7:TR?= MViwMVI1KGh? M2DmXYFkfGm4YYTld{Bk[XOyYYPlJFMwPw>? Mn;5NlYxPTV6MEW=
SK-ES-1 NX7nZoI6TnWwY4Tpc44hSXO|YYm= NIn2NYsyOCEQvF2= MkXjNE0zPCCq NUHTZohn[WO2aY\heIV{KGOjc4Dhd4UhOy95 M3HhSVI3ODV3OEC1
SK-N-MC  Mn;CSpVv[3Srb36gRZN{[Xl? NXrtdVV3OyEQvF2= NWjnXnhVOC1{NDDo NEGwfZJi[3SrdnH0[ZMh[2G|cHHz[UA{Nzd? NXqwbYkxOjZyNUW4NFU>
NRK-49F M1LlNGZ2dmO2aX;uJGF{e2G7 MmS0NQKBmzMEoN88US=> NYDBbmV1OzZiaB?= M2TQTIlv[3KnYYPld{BmgHC{ZYPzbY9vKG:oIN8xMXNOSSCjbnSg[oljem:wZXP0bY4h\G:|ZTDk[ZBmdmSnboTsfS=> NWLSNHVlOjZyMkKwNFM>
NRK-49F Mn3NSpVv[3Srb36gRZN{[Xl? MUew5qCUOsLizszN NV[yRW93OzZiaB?= MXXlcohidmOnczDwbI9{eGixconsZZRqd25ib3[gSWdHWiCjbnSgVGRITlMQstMg MUeyOlAzOjByMx?=
NRK-49F MojsSpVv[3Srb36gRZN{[Xl? M2f0XVDjiJN{wrFOwG0> M{XPWFM3KGh? NEnJ[m9mdmijbnPld{BUXEGWMzDwbI9{eGixconsZZRqd25? M2T2eFI3ODJ{MECz
RAW264.7 Mnf1SpVv[3Srb36gRZN{[Xl? M1vhSlEh|ryP MkHWOkBp Ml;5eZBz\We3bHH0[ZMhfGinIILl[JVk\WRiU1nSWFEheHKxdHXpckBweiCvUl7BJIxmfmWuczDifUBpcWeqIHfseYNwe2V? NYTJcIVOOjV5OUO5PVU>
MCF10A MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3XRVkxNTJyIN88US=> M3ThcVI1KGh? MnPQdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NH\zeHIzPTRzMUO1Oi=>
MCF-7 MkfUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoP4NE0zOCEQvF2= MWqyOEBp MVfy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> M2jsNlI2PDFzM{W2
T47D M{DaS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnyzNE0zOCEQvF2= MnP0NlQhcA>? MlS4doVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MmrINlU1OTF|NU[=
SKBR3 NXvpW4pbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPSNE0zOCEQvF2= NIGwcmczPCCq NHrxZopz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? MYqyOVQyOTN3Nh?=
MDA-MB-231 NHX1W3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUWwMVIxKM7:TR?= NGrXNHQzPCCq M4L5PZJm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NXXGTlNEOjV2MUGzOVY>
SUM149 NV3pWopPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUKwMVIxKM7:TR?= M4rmUlI1KGh? NWjYZ5JVemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NFnVb5QzPTRzMUO1Oi=>
HS578T M1K5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTUNE0zOCEQvF2= MlrTNlQhcA>? M1LsXZJm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? Mm\0NlU1OTF|NU[=
BT20 MnvkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonxNE0zOCEQvF2= MmPYNlQhcA>? MXjy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NVHuSXhtOjV2MUGzOVY>
A459 M3TsfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\lNE0zOCEQvF2= MkfSNlQhcA>? MWry[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NF\DeGczPTRzMUO1Oi=>
HCT116 NVjKU3JsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWGwMVIxKM7:TR?= NIrybHgzPCCq NUDHcJhxemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NWniN2ZbOjV2MUGzOVY>
Neu M{DxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\5R2ZLOC1{MDFOwG0> NIjjVoUzPCCq M1\JNJJm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? Ml\NNlU1OTF|NU[=
MDA-MB-231 NWPndGpZTnWwY4Tpc44hSXO|YYm= M1XCbVUh|ryP MUm4JIg> M{Lidolv[3KnYYPld{B1cGViboXtZoVzKG:oIHHjbYRq[yC4ZYPpZ5Vt[XJib4LnZY5mdGyncx?= M4rDWFI2PDFzM{W2
MDA-MB-231 NWmw[GJ[TnWwY4Tpc44hSXO|YYm= MVu1JO69VQ>? MkPKNVYhcA>? NFi2WVJqdmS3Y3XzJIx6e2:|b33hcEBu\W2kcnHu[UBx\XKvZXHibYxqgmG2aX;u NWnOZpN7OjV2MUGzOVY>
MC3T3-E1 NVXJXlRCTnWwY4Tpc44hSXO|YYm= NEDnNIkyOCEQvF2= NXTYbJlHPjBibXnuxsA> NXHuZpNNe3WycILld5NmeyC2aHWgSmdHNTJvc4TpcZVt[XSnZDDvd5Rmd3C{b4Tl[4VzcW5icnXs[YF{\Q>? MX:yOVI6ODB7NR?=
MC3T3-E1 MV3GeY5kfGmxbjDBd5NigQ>? NV\BR4NWOTBizszN NX7UUYdvPjBibXnuxsA> NVf5OJM{[XS2ZX71ZZRmeyC2aHWgSmdHNTJvaX7keYNm\CCxc4Tlc5Bzd3SnZ3XybY4hdVKQQTDlfJBz\XO|aX;u NUXkZYkxOjV{OUCwPVU>
MC3T3-E1 MnjMSpVv[3Srb36gRZN{[Xl? M4jYbVExKM7:TR?= M2nYO|YxKG2rbtMg MkD6ZZR1\W63YYTld{B1cGViRlfGMVIucW6mdXPl[EBwe3Snb4Dyc5Rm\2W{aX6gcXJPSSCneIDy[ZN{cW:w NVL6Xo11OjV{OUCwPVU>
MC3T3-E1 NFu4em1HfW6ldHnvckBCe3OjeR?= NFW1TVgyOCEQvF2= MofBOlAhdWmwwrC= MnfHd5VxeHKnc4Pld{B1cGViQl3QMVQue3SrbYXsZZRm\CCYRVfGJJJmdGWjc3W= MVGyOFQ{PTR2NB?=
MC3T3-E1 NFu4OGdHfW6ldHnvckBCe3OjeR?= M{nQVFExKM7:TR?= NEX2RlQ3OCCvaX9CpC=> Mmj2d5VxeHKnc4Pld{B1cGViUFfGNu6yNXO2aX31cIF1\WRiT2DHJJJmdGWjc3W= MV6yOFM{OzN|Nh?=
MC3T3-E1 NHLQ[I9HfW6ldHnvckBCe3OjeR?= M1LkWVExKM7:TR?= NFvnVpM3OCCvaX9CpC=> NXzqU2JsemWmdXPld{B1cGViUFfGNu6yNXO2aX31cIF1\WRicHjvd5Bpd3K7bHH0bY9vKG:oIIC0OE9xPDJiTVHQJItqdmG|ZR?= MUWyOFM{OzN|Nh?=
MC3T3-E1 NYfIXogzTnWwY4Tpc44hSXO|YYm= M3TLd|ExKM7:TR?= Mk\tOlAhdWmwwrC= MXXheJRmdnWjdHXzJJRp\SCSR1[y{tEucW6mdXPl[EBxcG:|cHjvdplt[XSrb36gc4Yh[m:2aDDNSWsyNzJiYX7kJHJi\i1z NHLUOmQzPDN|M{OzOi=>
RPE NH3lSIZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M3vCZ|UhyrWP M4jj[VEhcA>? MknNZZR1\W63YYTld{BQSc7{LXnu[JVk\WRiZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHl? MlyxNlQxOzZ7M{i=
9607 NI\kN3FE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NHPONIwyKM7:TR?= MXGzOkBp NXvROlhicW6lcnXhd4V{KHSqZTDj[YxtKH[rYXLpcIl1gSClb33wZZJm\CC5aYToJI1mdGG2b37pckBidG:wZR?= M37DOlI{PzJ4OUS5
9607 M3i4TmZ2dmO2aX;uJGF{e2G7 MVuxJO69VQ>? M2TSO|M3KGh? NUG3boRGcW6lcnXhd4V{KFOLUmSxJIFv\CCmZXPy[YF{\WRiYXPleJlt[XSnZD3wOVMh\XiycnXzd4lwdg>? NXWxcFk{OjN5Mk[5OFk>
RPMI.8226 MUHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MYe3M|ExKM7:TR?= MoH6NlQhcA>? NUnuU3VV\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 M3PPeVIyQTVyN{K4
U266 MlPER4VtdCCYaXHibYxqfHliQYPzZZk> M4raZ|cwOTBizszN NGm5V2kzPCCq MWfk[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= NWP5[lh1OjF7NUC3Nlg>
MM.1S MkCwR4VtdCCYaXHibYxqfHliQYPzZZk> NFnmO4Y4NzFyIN88US=> NGPlXGYzPCCq NFjJNlNl\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> MXSyNVk2ODd{OB?=
KMS12 NGH5TVJE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MYe3M|ExKM7:TR?= NF:zbmYzPCCq NUDWWpdr\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 NV:xeJVEOjF7NUC3Nlg>
LR5 M37BZ2NmdGxiVnnhZoltcXS7IFHzd4F6 M1zJVlcwOTBizszN NFG5UnEzPCCq NX7jbY5q\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 M3jQUlIyQTVyN{K4
MM.1R M3vQ[mNmdGxiVnnhZoltcXS7IFHzd4F6 M3\tblcwOTBizszN NUj1eJNrOjRiaB?= MWnk[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= MYCyNVk2ODd{OB?=
Ina6 NVmwNIRtS2WubDDWbYFjcWyrdImgRZN{[Xl? MoLJO{8yOCEQvF2= M37teFI1KGh? NET1OXdl\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NYTmV5ROOjF7NUC3Nlg>
RPMI-8226 NV\o[I5HSXCxcITvd4l{KEG|c3H5 NHHIb|U4NzFyIN88US=> NGfhdW4zPCCq M1fQPYlv\HWlZYOgZUB{cWewaX\pZ4FvfCCrbnPy[YF{\SCrbjD0bIUhSW6wZYjpckBXMy:SSfMIluKh[XCxcITvd4l{ MoKyNlE6PTB5Mki=
MM.1R  NEfpN5dCeG:ydH;zbZMhSXO|YYm= M3r1[FcwOTBizszN NWO3TpFIOjRiaB?= M{fRWolv\HWlZYOgZUB{cWewaX\pZ4FvfCCrbnPy[YF{\SCrbjD0bIUhSW6wZYjpckBXMy:SSfMIluKh[XCxcITvd4l{ MX:yNVk2ODd{OB?=
H411EC3 NYHnb|ZWTnWwY4Tpc44hSXO|YYm= M2frWVUxNzFyMDDuUS=> NUDuXIpkPiCq MnHFbY5kemWjc3XzJHNKWlRzIHHjeIl3cXS7IHnuJJRp\SCycnXz[Y5k\SCxZjDUV2EtKFCHUFPLJIFkfGm4aYT5MEBuWk6DIHzleoVteyCxZjDQZ4syKGGwZDDQ[4My|rFuIHHu[EBmdGW4YYTpcoch\2y3Y3;z[UBxem:mdXP0bY9v NYW2coRXOjF{MUKwPVY>
hepatocytes NV;RO2JGTnWwY4Tpc44hSXO|YYm= NVrUfndPOTBibl2= NHLXNW83KGh? MV3pcoNz\WG|ZYOgV2lTXDFiYXP0bZZqfHliaX6geIhmKHC{ZYPlcoNmKG:oIGTTRUwhWEWSQ1ugZYN1cX[rdImsJI1TVkFibHX2[Yx{KG:oIGDjb|Eh[W6mIGDnZ|HPuSxiYX7kJIVt\X[jdHnu[{BodHWlb4PlJJBzd2S3Y4Tpc44> NIjRb5czOTJzMkC5Oi=>
hepatocytes NH3nbFZHfW6ldHnvckBCe3OjeR?= MlfkNVAhdk1? NXLwbGRzPiCq MmHjbY5kemWjc3XzJGhu\2O{wrDhcoTDqEGlY9Mg[4Vv\SCneIDy[ZN{cW:w MXqyNVIyOjB7Nh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved-PARP-1 / Cleaved-caspase-3 / LC3-II / p62 / SIRT1; 

PubMed: 26655844     


SU86.86 cells were transfected with control siRNA, SIRT1 siRNA#1, or DBC1 siRNA. 24 hours after transfection, cells were re-plated and allowed to attach for 24 hours. Cells were then treated with vehicle (control) or 5 μM SRT1720 for 16 hours. Expression 䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙

26655844
Immunofluorescence
Cathepsin B; 

PubMed: 26655844     


SU86.86 cells were incubated with vehicle or 5 μM SRT1720 for 5 hours for LysoTracker Red labeling and 16 hours for cathepsin B immunofluorescence, followed by DAPI staining.

26655844
Growth inhibition assay
Cell viability ; 

PubMed: 25411356     


MTT viability assay of cancer cell lines treated with different doses of SRT1720 for 24 hours. 

25411356
In vivo In DIO mice SRT1720 mimics several of the effects observed after calorie restriction including improved insulin sensitivity, normalized glucose and insulin levels, and increased mitochondrial capacity. In addition, in diet-induced obese and genetically obese mice, SRT1720 improves insulin sensitivity, lower plasma glucose, and increase mitochondrial capacity. Thus, SRT1720 is a promising new therapeutic agent for treating diseases of ageing such as type 2 diabetes. Consistent with improved glucose tolerance, the glucose infusion rate required to maintain euglycaemia is approximately 35% higher in SRT1720-treated fa/fa rats, and the total glucose disposal rate is increased by approximately 20%. [1] SRT1720 also prevents multiple myeloma tumor growth. SRT1720 increases the cytotoxic activity of bortezomib or dexamethasone. [2]

Protocol

Kinase Assay:[1]
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SIRT1 fluorescence polarization assay:

In the SIRT1 FP assay, SIRT1 activity is monitored using a 20 amino acid peptide (Ac-Glu-Glu-Lys(biotin)-Gly-Gln-Ser-Thr-Ser-Ser-His-Ser-Lys(Ac)-Nle-Ser-Thr-Glu-Gly–Lys(MR121 or Tamra)-Glu-Glu-NH2) derived from the sequence of p53. The peptide is N-terminally linked to biotin and C-terminally modified with a fluorescent tag. The reaction for monitoring enzyme activity is a coupled enzyme assay where the first reaction is the deacetylation reaction catalyzed by SIRT1 and the second reaction is cleavage by trypsin at the newly exposed lysine residue. The reaction is stopped and streptavidin is added in order to accentuate the mass differences between substrate and product. The sensitivity of the FP assay allows identification of SRT1720. The fluorescence polarization reaction conditions are as follows: 0.5 μM peptide substrate, 150 μM βNAD+, 0-10 nM SIRT1, 25 mM Tris-acetate pH 8, 137 mM Na-Ac, 2.7 mM K-Ac, 1 mM Mg-Ac, 0.05% Tween-20, 0.1% Pluronic F127, 10 mM CaCl 2, 5 mM DTT, 0.025% BSA, and 0.15 mM nicotinamide. The reaction is incubated at 37 °C and stopped by addition of nicotinamide, and trypsin is added to cleave the deacetylated substrate. This reaction is incubated at 37 °C in the presence of 1 μM streptavidin. Fluorescent polarization is determined at excitation (650 nm) and emission (680 nm) wavelengths.
Cell Research:[2]
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  • Cell lines: Human vascular endothelial cells (HUVECs)
  • Concentrations: 5 μM
  • Incubation Time: 2 hours
  • Method: Transwell Insert Assays are utilized to measure migration. In vitro angiogenesis is assessed by Matrigel capillary-like tube structure formation assay. For endothelial tube formation assay, human vascular endothelial cells (HUVECs) are obtained from Clonetics and maintained in endothelial cell growth medium-2 containing 5% FBS. After three passages, HUVEC cell viability is measured with the trypan blue exclusion assay, and <5% of cell death is observed with SRT1720 treatment.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Chase-SCID mice with MM.1S cells
  • Dosages: 200 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 38 mg/mL (75.09 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 506.02
Formula

C25H23N7OS.HCl

CAS No. 1001645-58-4
Storage powder
in solvent
Synonyms N/A
Smiles C1CN(CCN1)CC2=CSC3=NC(=CN23)C4=CC=CC=C4NC(=O)C5=NC6=CC=CC=C6N=C5.Cl

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How can we prepare Srt1720 for in vivo mouse studies?

  • Answer:

    SRT1720 HCl can be dissolved in 30% PEG 400+0.5% Tween 80+5% Propylene glycol at 30mg/ml as a suspension. It is fine for oral gavage. And we’ve also found that it can be dissolved in 2% DMSO+30% PEG 300+1%Tween 80+ddH2O at 3mg/ml clearly, which could be used for injection. When prepare the solution, please dissolve the compound in DMSO clearly first, then add PEG and Tween. After they mixed well, dilute with water.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID