SRT1720 HCl

Catalog No.S1129

SRT1720 HCl Chemical Structure

Molecular Weight(MW): 506.02

SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3.

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In DMSO USD 238 In stock
USD 170 In stock
USD 320 In stock
USD 970 In stock
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5 Customer Reviews

  • EMBO J 2013 32, 791-804. SRT1720 HCl purchased from Selleck.

    PAI-1 expression in HUVECs treated with drugs as indicated.(D) senescent HUVECs were treated with SRT1720, culturing for 24, 48 hours. PAI-1 mRNA and protein (E) levels were analyzed using real-time RT–PCR and Western blotting, respectively. The RNA and protein levels were normalized to the internal control β-actin. Data are presented as the mean±SEM of three independent experiments. *P < 0.05 vs. corresponding control. **P < 0.01 vs. corresponding control ***P < 0.001 vs. corresponding control.

    Aging Cell 2014 13(5), 890-9. SRT1720 HCl purchased from Selleck.

  • Sirt1 deacetylase activity is essential for IRF9-mediated ischemic injury. The effects of SRT1720 on Sirt1 deacetylase activity in IRF9-KO and IRF9-TG mice, respectively. *p < 0.05 versus DMSO controls. n = 5.

    J Neurosci 2014 34(36), 11897-912. SRT1720 HCl purchased from Selleck.

    C2C12 myoblasts were transfected with si-CON and si-NDUFV1 for 24 h and then further differentiated into myotubes for 4 days in the absence or presence of pyruvate (25 mM), SRT1720 (2 uM), or resveratrol (Resv; 25 uM). Myogenesis was monitored using MyHC immunofluorescence and DAPI.

    J Biol Chem 2014 289(29), 20012-25. SRT1720 HCl purchased from Selleck.

  • J Biol Chem 2012 287, 19304-19314. SRT1720 HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Sirtuin Inhibitors

Biological Activity

Description SRT1720 HCl is a selective SIRT1 activator with EC50 of 0.16 μM in a cell-free assay, but is >230-fold less potent for SIRT2 and SIRT3.
Targets
SIRT1 [1]
(Cell-free assay)
0.16 μM(EC50)
In vitro

The maximum activation ratio of SRT1720 versus the closest sirtuin homologues, SIRT2 (EC1.5 = 37 μM) and SIRT3 (EC1.5 > 300 μM) is up to 781%. SRT1720 binds to the SIRT1 enzyme-peptide substrate complex at an allosteric site amino-terminal to the catalytic domain and lower the Michaelis constant for acetylated substrates. SRT1720 could reduce fed glucose levels. Glucose excursion during an intraperitoneal glucose tolerance test is also significantly reduced in the SRT1720 group, and comparable to rosiglitazone, a PPARγ activator that has been used to treat type 2 diabetes. SRT1720 does not have an effect on fasting glucose in chow-fed mice, revealing that pharmacological SIRT1 activation is unlikely to induce hypoglycaemia. SRT1720 significantly reduces the hyperinsulinaemia after 4 weeks, partially normalizing increased insulin levels similar to rosiglitazone treatment. SRT1720 treatment increases mitochondrial capacity by 15% in gastrocnemius muscle as measured by citrate synthase activity. [1] Higher concentrations of SRT1720 (15 μM) induces a modest (10-20%) decrease in normal cell viability. SRT1720 also significantly inhibits VEGF-dependent MM cell migration. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CACs  M13ZR2Z2dmO2aX;uJGF{e2G7 NVXzU2xIPMLizszN MV:zNOKhdWmw MV7EUXNQ Ml;wbY5lfWOnczDhZ5V1\SCVSWLUNUBi[3SrdnH0bY9vyqB? NY\xSYhbOjZ{NUSxNFQ>
MC3T3-E1 NVjrd2x{TnWwY4Tpc44hSXO|YYm= M2\mTlExKML3TdMg NHLte5UyKGh? MmnudoVlfWOnczD0bIUhXEeILd8yMZN1cW23bHH0[YQhXkWJRjDy[Yxm[XOnIHnuJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= MUeyOlE{Pjl5OB?=
MC3T3-E1 M3PPZmZ2dmO2aX;uJGF{e2G7 NF\hfGQyOCEEtV5CpC=> Ml3HNVIhcA>? M1XGSJJm\HWlZYOgeIhmKF[HR1[gcXJPSSCneIDy[ZN{cW:wIHzleoVteyC|dHnteYxifGWmIHL5JHRITi4Qsh?= NWjwTXdqOjZzM{[5O|g>
MC3T3-E1 NVjwS5lKTnWwY4Tpc44hSXO|YYm= M2\0V|IxKM7:TR?= NX:4NGlQOSCq MWDzeZBxemW|c3XzJJRp\SCWR1[t{tIucW6mdXPl[EBxcG:|cHjvdplt[XSrb36gc4YheDR2L4C0NkBOSVBia3nuZZNmKG:{IGPBVGswUk6N NXzDUohCOjZzM{[5O|g>
WE-68 MkToRZBweHSxc3nzJGF{e2G7 Ml;UNE0zPCEQvF2= M{f2WVI1KGh? MlzQbY5lfWOnczDj[YxtKGSnYYToJIlvKGSxc3Wg[IVx\W6mZX70cJk> M{m2OVI3ODV3OEC1
SK-ES-1 NXTZVIMxSXCxcITvd4l{KEG|c3H5 MY[wMVExKM7:TR?= NYHnVZNyOjRiaB?= NFLHWYRqdmS3Y3XzJINmdGxiZHXheIghcW5iZH;z[UBl\XCnbnTlcpRtgQ>? NXrBbJBMOjZyNUW4NFU>
SK-N-MC  NVPqOnM6SXCxcITvd4l{KEG|c3H5 NXjzcIdqOC1{LkWg{txO NFr2bIszPCCq MUPpcoR2[2W|IHPlcIwh\GWjdHigbY4h\G:|ZTDk[ZBmdmSnboTsfS=> M2LkR|I3ODV3OEC1
WE-68 M4nNdGZ2dmO2aX;uJGF{e2G7 NGTvXpUzOCEQvF2= NXTYTXgzOC1{NDDo NULiRXR{[WO2aY\heIV{KGOjc4Dhd4UhOy95 MUmyOlA2PThyNR?=
SK-ES-1 NIrwSWdHfW6ldHnvckBCe3OjeR?= M1TLUVExKM7:TR?= NF\DV4wxNTJ2IHi= MYDhZ5RqfmG2ZYOgZ4F{eGG|ZTCzM|c> NU[ydZdDOjZyNUW4NFU>
SK-N-MC  NY\6SYhqTnWwY4Tpc44hSXO|YYm= M3TNTlMh|ryP NV\YSm5QOC1{NDDo MV;hZ5RqfmG2ZYOgZ4F{eGG|ZTCzM|c> MUSyOlA2PThyNR?=
NRK-49F NV33N3BFTnWwY4Tpc44hSXO|YYm= NXTaOWxoOOLCk{NCpO69VQ>? MmPTN|YhcA>? M37tOYlv[3KnYYPld{BmgHC{ZYPzbY9vKG:oIN8xMXNOSSCjbnSg[oljem:wZXP0bY4h\G:|ZTDk[ZBmdmSnboTsfS=> MoiyNlYxOjJyMEO=
NRK-49F M{HjZ2Z2dmO2aX;uJGF{e2G7 NXzIVFJoOOLCk{NCpO69VQ>? M3K5eFM3KGh? Mnmz[Y5p[W6lZYOgdIhwe3Cqb4L5cIF1cW:wIH;mJGVITlJiYX7kJHBFT0[UzsNCpC=> NUTaUYRGOjZyMkKwNFM>
NRK-49F NWTxSJc3TnWwY4Tpc44hSXO|YYm= MWCw5qCUOsLizszN MUCzOkBp NYPoelFL\W6qYX7j[ZMhW1SDVEOgdIhwe3Cqb4L5cIF1cW:w MoHtNlYxOjJyMEO=
RAW264.7 NFvaRppHfW6ldHnvckBCe3OjeR?= MnjGNUDPxE1? M3XifFYhcA>? NGHmV5B2eHKnZ4XsZZRmeyC2aHWgdoVlfWOnZDDTTXJVOSCycn;0[YlvKG:{IH3SUmEhdGW4ZXzzJIJ6KGirZ3ig[4x2[2:|ZR?= NVPZW5dJOjV5OUO5PVU>
MCF10A M2f1SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7jNE0zOCEQvF2= MWiyOEBp M4m0VJJm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NUL6XoJvOjV2MUGzOVY>
MCF-7 M3rKTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYKwMVIxKM7:TR?= MkfFNlQhcA>? NVnyUGE4emWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 Mk\1NlU1OTF|NU[=
T47D NHfTNWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4[3OFAuOjBizszN MV[yOEBp MWny[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NYHLU|k1OjV2MUGzOVY>
SKBR3 NUHxUHFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnjOmVEOC1{MDFOwG0> M3vlN|I1KGh? NYfvenpbemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 Mnm2NlU1OTF|NU[=
MDA-MB-231 M{TQe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zKclAuOjBizszN NV;6ZnplOjRiaB?= MmPOdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NEXNc3gzPTRzMUO1Oi=>
SUM149 M1i4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGP4em4xNTJyIN88US=> MYmyOEBp MXLy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGSxc3Wg[IVx\W6mZX70cJk> NVnjO2ZnOjV2MUGzOVY>
HS578T MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnlNE0zOCEQvF2= NYTxToV5OjRiaB?= M2j0VpJm\HWlZYOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? MYGyOVQyOTN3Nh?=
BT20 M{jScGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkO1NE0zOCEQvF2= NWnrVHp1OjRiaB?= NFGzcXJz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? NUPucnpbOjV2MUGzOVY>
A459 M{P1UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXZb2oxNTJyIN88US=> NFfuNXYzPCCq NX33NI1LemWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NET3NoozPTRzMUO1Oi=>
HCT116 M1jwdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITjZYMxNTJyIN88US=> NF7TblYzPCCq NVLYXWh4emWmdXPld{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NFq1WnAzPTRzMUO1Oi=>
Neu NXXNU3dHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLJSpQxNTJyIN88US=> M4jHZVI1KGh? NGTaSnBz\WS3Y3XzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M3v1TlI2PDFzM{W2
MDA-MB-231 MYjGeY5kfGmxbjDBd5NigQ>? NVTDbmJUPSEQvF2= NI\MfWE5KGh? NEHB[HRqdmO{ZXHz[ZMhfGinIH71cYJmeiCxZjDhZ4llcWNidnXzbYN2dGG{IH;y[4Fv\WyuZYO= MlTUNlU1OTF|NU[=
MDA-MB-231 M3z0XWZ2dmO2aX;uJGF{e2G7 NIHNNHM2KM7:TR?= MVuxOkBp NHzGd5JqdmS3Y3XzJIx6e2:|b33hcEBu\W2kcnHu[UBx\XKvZXHibYxqgmG2aX;u MUGyOVQyOTN3Nh?=
MC3T3-E1 NFHrTlFHfW6ldHnvckBCe3OjeR?= NGfiN3MyOCEQvF2= M2iyeFYxKG2rbtMg NHjoNJJ{fXCycnXzd4V{KHSqZTDGS2YuOi2|dHnteYxifGWmIH;zeIVweHKxdHXn[ZJqdiC{ZXzlZZNm NUjoU|BlOjV{OUCwPVU>
MC3T3-E1 Mmf2SpVv[3Srb36gRZN{[Xl? NXr6R3NlOTBizszN M{G4XFYxKG2rbtMg MYrheJRmdnWjdHXzJJRp\SCIR1[tNk1qdmS3Y3XkJI9{fGWxcILveIVo\XKrbjDtVm5CKGW6cILld5Nqd25? NFXpWIwzPTJ7MEC5OS=>
MC3T3-E1 NH3XZ3FHfW6ldHnvckBCe3OjeR?= NXzrR5RMOTBizszN MW[2NEBucW8EoB?= MlzDZZR1\W63YYTld{B1cGViRlfGMVIucW6mdXPl[EBwe3Snb4Dyc5Rm\2W{aX6gcXJPSSCneIDy[ZN{cW:w M{PZeVI2OjlyMEm1
MC3T3-E1 NF:yUm5HfW6ldHnvckBCe3OjeR?= NYf3VHlrOTBizszN M1jLe|YxKG2rbtMg NE\ScW5{fXCycnXzd4V{KHSqZTDCUXAuPC2|dHnteYxifGWmIG\FS2YhemWuZXHz[S=> NXTGUHlROjR2M{W0OFQ>
MC3T3-E1 M1XwRWZ2dmO2aX;uJGF{e2G7 M1\5bVExKM7:TR?= MXe2NEBucW8EoB?= NHy5eJN{fXCycnXzd4V{KHSqZTDQS2Yz|rFvc4TpcZVt[XSnZDDPVGchemWuZXHz[S=> MoG1NlQ{OzN|M{[=
MC3T3-E1 NWjrdppLTnWwY4Tpc44hSXO|YYm= NETyOmYyOCEQvF2= NHLCTYM3OCCvaX9CpC=> MXTy[YR2[2W|IITo[UBRT0Z{zsGtd5RqdXWuYYTl[EBxcG:|cHjvdplt[XSrb36gc4YheDR2L4C0NkBOSVBia3nuZZNm NV7Db|B2OjR|M{OzN|Y>
MC3T3-E1 MYDGeY5kfGmxbjDBd5NigQ>? NIDmN|AyOCEQvF2= NU\KVmtlPjBibXnuxsA> NFfLS3difHSnboXheIV{KHSqZTDQS2Yz|rFvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiYn;0bEBOTUtzL{KgZY5lKFKjZj2x MnHRNlQ{OzN|M{[=
RPE NX7HZnpjS2WubDDWbYFjcWyrdImgRZN{[Xl? MkjZOUDDvU1? NX\2cIF5OSCq M4f0U4F1fGWwdXH0[ZMhV0IQsj3pcoR2[2WmIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7 MVyyOFA{Pjl|OB?=
9607 NYjwO|NGS2WubDDWbYFjcWyrdImgRZN{[Xl? NFrvS|UyKM7:TR?= NWLWfoxEOzZiaB?= NULUSYNvcW6lcnXhd4V{KHSqZTDj[YxtKH[rYXLpcIl1gSClb33wZZJm\CC5aYToJI1mdGG2b37pckBidG:wZR?= M1HZOFI{PzJ4OUS5
9607 NEXIbIlHfW6ldHnvckBCe3OjeR?= MX:xJO69VQ>? M{PVZVM3KGh? NHLjSXJqdmO{ZXHz[ZMhW0mUVEGgZY5lKGSnY4LlZZNm\CCjY3X0fYxifGWmLYC1N{BmgHC{ZYPzbY9v NY\wbnFZOjN5Mk[5OFk>
RPMI.8226 NIq3U49E\WyuIG\pZYJqdGm2eTDBd5NigQ>? M4P5TlcwOTBizszN NUfCNZJDOjRiaB?= NVG1eZRH\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 MW[yNVk2ODd{OB?=
U266 M1ywOWNmdGxiVnnhZoltcXS7IFHzd4F6 NHS0c284NzFyIN88US=> MVmyOEBp NFnDR|hl\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NH7kfoEzOTl3MEeyPC=>
MM.1S NWHjO4dmS2WubDDWbYFjcWyrdImgRZN{[Xl? MnvJO{8yOCEQvF2= MVKyOEBp MWfk[YNz\WG|ZYOgeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= MlXiNlE6PTB5Mki=
KMS12 NGPYZ4ZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MWO3M|ExKM7:TR?= MWeyOEBp NGLi[4ll\WO{ZXHz[ZMhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NWfLSIE4OjF7NUC3Nlg>
LR5 MnnSR4VtdCCYaXHibYxqfHliQYPzZZk> M17tVFcwOTBizszN Mk\tNlQhcA>? NUO1R5Rt\GWlcnXhd4V{KH[rYXLpcIl1gSClb37j[Y51emG2aX;uJIRmeGWwZHXueIx6 NHnpb4IzOTl3MEeyPC=>
MM.1R MWfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> Mor1O{8yOCEQvF2= MXyyOEBp M1PSOoRm[3KnYYPld{B3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> M2HVWlIyQTVyN{K4
Ina6 MVnD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MUO3M|ExKM7:TR?= NH3jUpgzPCCq M1H1foRm[3KnYYPld{B3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> MoDaNlE6PTB5Mki=
RPMI-8226 MlfwRZBweHSxc3nzJGF{e2G7 NHHtbVk4NzFyIN88US=> M1HobFI1KGh? NXTEd5d5cW6mdXPld{BiKHOrZ37p[olk[W62IHnuZ5Jm[XOnIHnuJJRp\SCDbn7lfIlvKFZtL2DJ5qiTyqCjcH;weI9{cXN? NF;PSW8zOTl3MEeyPC=>
MM.1R  NGP0U|NCeG:ydH;zbZMhSXO|YYm= MXW3M|ExKM7:TR?= NESxSXgzPCCq Ml6ybY5lfWOnczDhJJNq\26rZnnjZY51KGmwY4LlZZNmKGmwIITo[UBCdm6neHnuJHYsN1CL4pkSxsBieG:ydH;zbZM> MkfoNlE6PTB5Mki=
H411EC3 MoO3SpVv[3Srb36gRZN{[Xl? NX;jdZNWPTBxMUCwJI5O MlHkOkBp M4PYR4lv[3KnYYPld{BUUVKWMTDhZ5Rqfmm2eTDpckB1cGVicILld4Vv[2Vib3[gWHNCNCCSRWDDT{Bi[3Srdnn0fUwhdVKQQTDs[ZZmdHNib3[gVINsOSCjbnSgVIdkOc7zLDDhcoQh\WyndnH0bY5oKGeudXPvd4UheHKxZIXjeIlwdg>? NEfSbpozOTJzMkC5Oi=>
hepatocytes NETkWVZHfW6ldHnvckBCe3OjeR?= NGrRW2wyOCCwTR?= NXHiOXJwPiCq MYPpcoNz\WG|ZYOgV2lTXDFiYXP0bZZqfHliaX6geIhmKHC{ZYPlcoNmKG:oIGTTRUwhWEWSQ1ugZYN1cX[rdImsJI1TVkFibHX2[Yx{KG:oIGDjb|Eh[W6mIGDnZ|HPuSxiYX7kJIVt\X[jdHnu[{BodHWlb4PlJJBzd2S3Y4Tpc44> NXPlXGFMOjF{MUKwPVY>
hepatocytes NEjmVopHfW6ldHnvckBCe3OjeR?= NVjWTnVpOTBibl2= NYrFRZI4PiCq NGj0eHRqdmO{ZXHz[ZMhUG2pY4NCpIFv\MLiQXPjxsBo\W6nIHX4dJJme3Orb36= NIHvZnIzOTJzMkC5Oi=>

... Click to View More Cell Line Experimental Data

In vivo In DIO mice SRT1720 mimics several of the effects observed after calorie restriction including improved insulin sensitivity, normalized glucose and insulin levels, and increased mitochondrial capacity. In addition, in diet-induced obese and genetically obese mice, SRT1720 improves insulin sensitivity, lower plasma glucose, and increase mitochondrial capacity. Thus, SRT1720 is a promising new therapeutic agent for treating diseases of ageing such as type 2 diabetes. Consistent with improved glucose tolerance, the glucose infusion rate required to maintain euglycaemia is approximately 35% higher in SRT1720-treated fa/fa rats, and the total glucose disposal rate is increased by approximately 20%. [1] SRT1720 also prevents multiple myeloma tumor growth. SRT1720 increases the cytotoxic activity of bortezomib or dexamethasone. [2]

Protocol

Kinase Assay:[1]
+ Expand

SIRT1 fluorescence polarization assay:

In the SIRT1 FP assay, SIRT1 activity is monitored using a 20 amino acid peptide (Ac-Glu-Glu-Lys(biotin)-Gly-Gln-Ser-Thr-Ser-Ser-His-Ser-Lys(Ac)-Nle-Ser-Thr-Glu-Gly–Lys(MR121 or Tamra)-Glu-Glu-NH2) derived from the sequence of p53. The peptide is N-terminally linked to biotin and C-terminally modified with a fluorescent tag. The reaction for monitoring enzyme activity is a coupled enzyme assay where the first reaction is the deacetylation reaction catalyzed by SIRT1 and the second reaction is cleavage by trypsin at the newly exposed lysine residue. The reaction is stopped and streptavidin is added in order to accentuate the mass differences between substrate and product. The sensitivity of the FP assay allows identification of SRT1720. The fluorescence polarization reaction conditions are as follows: 0.5 μM peptide substrate, 150 μM βNAD+, 0-10 nM SIRT1, 25 mM Tris-acetate pH 8, 137 mM Na-Ac, 2.7 mM K-Ac, 1 mM Mg-Ac, 0.05% Tween-20, 0.1% Pluronic F127, 10 mM CaCl 2, 5 mM DTT, 0.025% BSA, and 0.15 mM nicotinamide. The reaction is incubated at 37 °C and stopped by addition of nicotinamide, and trypsin is added to cleave the deacetylated substrate. This reaction is incubated at 37 °C in the presence of 1 μM streptavidin. Fluorescent polarization is determined at excitation (650 nm) and emission (680 nm) wavelengths.
Cell Research:[2]
+ Expand
  • Cell lines: Human vascular endothelial cells (HUVECs)
  • Concentrations: 5 μM
  • Incubation Time: 2 hours
  • Method: Transwell Insert Assays are utilized to measure migration. In vitro angiogenesis is assessed by Matrigel capillary-like tube structure formation assay. For endothelial tube formation assay, human vascular endothelial cells (HUVECs) are obtained from Clonetics and maintained in endothelial cell growth medium-2 containing 5% FBS. After three passages, HUVEC cell viability is measured with the trypan blue exclusion assay, and <5% of cell death is observed with SRT1720 treatment.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Chase-SCID mice with MM.1S cells
  • Formulation: 20% PEG400/0.5% Tween80/79.5% deionized water
  • Dosages: 200 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 38 mg/mL (75.09 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% Propylene glycol
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 506.02
Formula

C25H23N7OS.HCl

CAS No. 1001645-58-4
Storage powder
in solvent
Synonyms N/A

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How can we prepare Srt1720 for in vivo mouse studies?

  • Answer:

    SRT1720 HCl can be dissolved in 30% PEG 400+0.5% Tween 80+5% Propylene glycol at 30mg/ml as a suspension. It is fine for oral gavage. And we’ve also found that it can be dissolved in 2% DMSO+30% PEG 300+1%Tween 80+ddH2O at 3mg/ml clearly, which could be used for injection. When prepare the solution, please dissolve the compound in DMSO clearly first, then add PEG and Tween. After they mixed well, dilute with water.

Sirtuin Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID